Standard name | |
---|---|
Human Ortholog | |
Description | Essential RNA-binding G protein effector of mating response pathway; ligand-activated RNA-binding protein that delivers RNAs involved in polarization and perpetualizing mating signal to shmoo tip during pheromone signaling; Scp160p-mediated RNA trafficking essential for chemotropism and successful mating; mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 15 | 12 | 11 | 11 | 20 | 17 | 24 | 24 | 20 | 0 | 0 | 0 | 1 | 1 | 0 | 5 | 3 | 9 | 1 | 4 | 3 |
Bud | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 7 | 11 | 10 | 17 | 11 | 15 | 18 | 20 | 17 | 16 | 17 | 4 | 4 | 11 | 16 | 8 | 18 | 2 | 0 | 1 | 2 | 2 | 2 |
Cytoplasm | 310 | 196 | 77 | 27 | 34 | 55 | 65 | 70 | 62 | 64 | 67 | 41 | 80 | 80 | 136 | 125 | 141 | 111 | 135 | 90 | 16 | 12 | 21 | 24 |
Endoplasmic Reticulum | 234 | 117 | 244 | 157 | 204 | 204 | 204 | 389 | 176 | 205 | 159 | 149 | 35 | 40 | 21 | 1 | 5 | 3 | 382 | 212 | 287 | 122 | 176 | 178 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 |
Golgi | 9 | 0 | 32 | 75 | 101 | 30 | 29 | 43 | 15 | 22 | 19 | 37 | 1 | 0 | 0 | 3 | 4 | 0 | 5 | 1 | 17 | 28 | 20 | 19 |
Mitochondria | 0 | 0 | 1 | 5 | 0 | 4 | 2 | 27 | 12 | 20 | 31 | 35 | 0 | 0 | 0 | 2 | 5 | 0 | 0 | 0 | 5 | 0 | 1 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 0 | 0 | 2 | 8 | 6 | 3 | 3 | 3 | 3 | 2 | 3 | 3 | 3 | 0 | 3 | 2 | 0 | 4 | 1 | 0 | 3 | 2 | 2 |
Unique Cell Count | 499 | 300 | 331 | 235 | 314 | 283 | 297 | 509 | 258 | 301 | 261 | 253 | 110 | 113 | 151 | 144 | 154 | 123 | 554 | 320 | 345 | 180 | 235 | 241 |
Labelled Cell Count | 560 | 320 | 366 | 291 | 376 | 323 | 332 | 578 | 305 | 358 | 321 | 310 | 123 | 127 | 168 | 155 | 166 | 134 | 554 | 320 | 345 | 180 | 235 | 241 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 73.3 | 83.1 | 67.0 | 58.7 | 56.9 | 51.6 | 48.6 | 45.6 | 44.2 | 41.8 | 39.4 | 39.8 | 51.3 | 51.3 | 57.4 | 45.9 | 56.1 | 68.2 | 59.7 | 68.2 | 73.5 |
Std Deviation (1e-4) | 13.7 | 15.1 | 12.6 | 12.7 | 11.1 | 10.1 | 10.6 | 9.8 | 9.1 | 9.2 | 8.2 | 9.2 | 13.6 | 13.6 | 14.4 | 17.7 | 21.2 | 21.4 | 15.5 | 16.4 | 17.6 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.24 | -0.38 | -0.46 | -0.56 | -0.6 | -0.68 | -0.77 | -0.75 | -0.39 | -0.39 | -0.22 | -0.54 | -0.25 | 0.03 | -0.17 | 0.03 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 4.3 | 3.2 | 3.2 | 3.1 | 3.3 | 4.4 | 4.9 | 5.3 | 4.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.6 | 1.3 | 0.4 | 1.1 | 0.2 | 2.4 | 1.4 | 1.6 | 1.9 | 0 | 0 | 1.9 | 3.4 | 1.0 | 4.4 |
Cytoplasm | -3.6 | -4.2 | -1.2 | -0.4 | -3.5 | 0.2 | -0.6 | 0.7 | -2.1 | 9.4 | 9.1 | 13.7 | 12.9 | 14.1 | 12.9 |
Endoplasmic Reticulum | -1.8 | -2.4 | -0.5 | -1.4 | 0.9 | -1.5 | -1.6 | -3.3 | -3.8 | -7.9 | -7.3 | -12.2 | -14.6 | -14.5 | -13.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 6.7 | 7.1 | 0.4 | 0 | -0.6 | -1.7 | -1.1 | -1.0 | 1.8 | -3.0 | -3.4 | -4.0 | -2.9 | -2.8 | -3.6 |
Mitochondria | 0 | 0 | 0 | 0 | 3.9 | 3.6 | 4.4 | 6.2 | 6.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0269 | 0.0152 | 0.0242 | 0.0657 | 0.0799 | 0.0212 | 0.0392 | 0.0169 | 0.0262 | 0.003 | 0.0068 | 0.026 | 0.0417 | 0.0037 | 0.0001 | 0.0859 | 0.0763 | 0.0007 |
Bud | 0.0005 | 0.0007 | 0.0004 | 0.009 | 0.0015 | 0.002 | 0.0015 | 0.0031 | 0.0006 | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0 | 0 | 0.0057 | 0.0066 | 0 |
Bud Neck | 0.0007 | 0.0017 | 0.0029 | 0.0047 | 0.0069 | 0.0071 | 0.001 | 0.0013 | 0.0012 | 0.0012 | 0.0019 | 0.003 | 0.0002 | 0.0003 | 0 | 0.0177 | 0.0091 | 0.0003 |
Bud Periphery | 0.0011 | 0.0012 | 0.0007 | 0.0082 | 0.0012 | 0.0024 | 0.0023 | 0.0029 | 0.0013 | 0.0001 | 0.0018 | 0.0002 | 0.0005 | 0 | 0 | 0.0041 | 0.0043 | 0 |
Bud Site | 0.0011 | 0.0026 | 0.0004 | 0.0434 | 0.0071 | 0.0003 | 0.0019 | 0.018 | 0.0021 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0.0005 | 0.0007 | 0 |
Cell Periphery | 0.0059 | 0.041 | 0.0159 | 0.011 | 0.0269 | 0.0076 | 0.0116 | 0.0142 | 0.01 | 0.001 | 0.0024 | 0.0018 | 0.0002 | 0.0002 | 0.0003 | 0.0038 | 0.0153 | 0.0005 |
Cytoplasm | 0.1193 | 0.2668 | 0.1834 | 0.1795 | 0.1888 | 0.1398 | 0.079 | 0.1923 | 0.1526 | 0.1288 | 0.2069 | 0.0677 | 0.0429 | 0.5273 | 0.0007 | 0.6427 | 0.2059 | 0.0032 |
Cytoplasmic Foci | 0.0243 | 0.0062 | 0.0097 | 0.0091 | 0.0249 | 0.0032 | 0.0143 | 0.0084 | 0.0089 | 0.001 | 0.0053 | 0.0035 | 0.0374 | 0.0029 | 0 | 0.0104 | 0.0049 | 0.0001 |
Eisosomes | 0.0006 | 0.0028 | 0.0009 | 0.002 | 0.0045 | 0.0018 | 0.0024 | 0.0007 | 0.001 | 0.0001 | 0.0003 | 0.0006 | 0.0022 | 0 | 0 | 0.0001 | 0.0085 | 0.0001 |
Endoplasmic Reticulum | 0.3522 | 0.4243 | 0.5165 | 0.3151 | 0.231 | 0.4218 | 0.3952 | 0.5549 | 0.5998 | 0.7918 | 0.4782 | 0.5797 | 0.0073 | 0.3342 | 0.9357 | 0.0682 | 0.2825 | 0.5036 |
Endosome | 0.0244 | 0.0116 | 0.0139 | 0.0354 | 0.0249 | 0.0227 | 0.0365 | 0.0242 | 0.0205 | 0.0066 | 0.072 | 0.0253 | 0.0606 | 0.0047 | 0.0006 | 0.0651 | 0.0321 | 0.0025 |
Golgi | 0.0123 | 0.0248 | 0.0314 | 0.0361 | 0.0257 | 0.0185 | 0.0264 | 0.0359 | 0.0312 | 0.0086 | 0.044 | 0.0438 | 0.0109 | 0.0013 | 0.0004 | 0.0292 | 0.0859 | 0.0042 |
Lipid Particles | 0.0182 | 0.0214 | 0.0195 | 0.0129 | 0.0315 | 0.0081 | 0.0167 | 0.0146 | 0.0122 | 0.0003 | 0.0025 | 0.0107 | 0.0479 | 0.0009 | 0 | 0.0014 | 0.0041 | 0.0002 |
Mitochondria | 0.01 | 0.0047 | 0.0054 | 0.0167 | 0.0097 | 0.0027 | 0.0251 | 0.0021 | 0.0093 | 0.0006 | 0.0404 | 0.0018 | 0.0085 | 0.0001 | 0.0001 | 0.003 | 0.0248 | 0.0003 |
None | 0.0073 | 0.0017 | 0.0059 | 0.0014 | 0.0113 | 0.001 | 0.0138 | 0.0005 | 0.0017 | 0.0002 | 0.0011 | 0.0004 | 0.6041 | 0.0005 | 0 | 0.0012 | 0.002 | 0.0001 |
Nuclear Periphery | 0.3319 | 0.1327 | 0.1328 | 0.176 | 0.2411 | 0.3079 | 0.2497 | 0.0898 | 0.0984 | 0.0457 | 0.0916 | 0.2143 | 0.058 | 0.1158 | 0.0583 | 0.0286 | 0.1987 | 0.467 |
Nucleolus | 0.0011 | 0.0053 | 0.0006 | 0.0009 | 0.007 | 0.0003 | 0.0019 | 0.0006 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0041 | 0 | 0 | 0.0003 | 0.0009 | 0 |
Nucleus | 0.0197 | 0.0102 | 0.007 | 0.0095 | 0.0136 | 0.0121 | 0.0187 | 0.0039 | 0.0035 | 0.0016 | 0.0035 | 0.0035 | 0.0074 | 0.0038 | 0.0004 | 0.0074 | 0.0074 | 0.0072 |
Peroxisomes | 0.0056 | 0.0002 | 0.0015 | 0.001 | 0.0051 | 0.0002 | 0.017 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0012 | 0.0173 | 0 | 0 | 0.0002 | 0.0004 | 0 |
Punctate Nuclear | 0.0104 | 0.0013 | 0.0024 | 0.0013 | 0.0141 | 0.0014 | 0.011 | 0.0006 | 0.0006 | 0.0001 | 0.0003 | 0.0007 | 0.0401 | 0.0011 | 0 | 0.001 | 0.0009 | 0.0003 |
Vacuole | 0.0123 | 0.0168 | 0.0167 | 0.0219 | 0.0199 | 0.0076 | 0.0156 | 0.0095 | 0.0079 | 0.0051 | 0.0129 | 0.0036 | 0.0029 | 0.002 | 0.0009 | 0.0142 | 0.0054 | 0.0012 |
Vacuole Periphery | 0.0141 | 0.0069 | 0.0078 | 0.0392 | 0.0234 | 0.0103 | 0.0191 | 0.0053 | 0.0101 | 0.0038 | 0.0272 | 0.0119 | 0.0051 | 0.0011 | 0.0025 | 0.0095 | 0.0232 | 0.0086 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 239.5371 | 243.8384 | 228.0186 | 227.8882 | 228.4924 | 196.8738 | 218.9546 | 193.2785 | 170.5598 | 215.0478 |
Translational Efficiency | 1.9577 | 1.9686 | 1.6803 | 1.8449 | 1.9029 | 2.1327 | 1.6143 | 1.5543 | 1.6365 | 1.642 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2035 | 1018 | 165 | 1079 | 1185 | 1425 | 2079 | 1753 | 3220 | 2443 | 2244 | 2832 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 4519.20 | 3880.95 | 5619.01 | 4558.04 | 2705.15 | 3827.29 | 5535.67 | 4868.74 | 3851.61 | 3849.65 | 5541.80 | 4750.36 |
Standard Deviation | 1014.68 | 925.94 | 840.83 | 1231.05 | 683.63 | 799.13 | 872.12 | 1001.85 | 1260.17 | 854.67 | 870.13 | 1105.20 |
Intensity Change Log 2 | — | -0.219658 | 0.314249 | 0.012346 | — | 0.500615 | 1.033049 | 0.847840 | — | 0.093534 | 0.626709 | 0.383897 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001795 | 0.001742 | 0.000580 | 0.002679 | 0.002606 | 0.000686 | 0.000595 | 0.002034 | 0.002093 | 0.001126 | 0.000594 | 0.002280 |
Bud Neck | 0.001988 | 0.002074 | 0.021248 | 0.005016 | 0.001043 | 0.001226 | 0.001108 | 0.001711 | 0.001640 | 0.001579 | 0.002589 | 0.002970 |
Bud Site | 0.009203 | 0.008488 | 0.004374 | 0.036910 | 0.005823 | 0.004039 | 0.001599 | 0.014608 | 0.007959 | 0.005893 | 0.001803 | 0.023105 |
Cell Periphery | 0.028606 | 0.015213 | 0.038833 | 0.096187 | 0.012998 | 0.014225 | 0.035666 | 0.093228 | 0.022862 | 0.014637 | 0.035899 | 0.094355 |
Cytoplasm | 0.035702 | 0.017650 | 0.010788 | 0.114033* | 0.005549 | 0.009814 | 0.010841 | 0.063746 | 0.024605 | 0.013079 | 0.010837 | 0.082905 |
Cytoplasmic Foci | 0.010150 | 0.014002 | 0.002891 | 0.017713 | 0.010139 | 0.010776 | 0.002909 | 0.022838 | 0.010146 | 0.012120 | 0.002908 | 0.020885 |
Eisosomes | 0.000146 | 0.000297 | 0.000462 | 0.000155 | 0.000343 | 0.000133 | 0.000195 | 0.000220 | 0.000219 | 0.000201 | 0.000214 | 0.000195 |
Endoplasmic Reticulum | 0.475749* | 0.528715* | 0.199369* | 0.070909 | 0.545345* | 0.647077* | 0.732798* | 0.322867* | 0.501361* | 0.597755* | 0.693575* | 0.226870* |
Endosome | 0.020096 | 0.024363 | 0.015965 | 0.010639 | 0.017067 | 0.021940 | 0.007683 | 0.012566 | 0.018982 | 0.022950 | 0.008292 | 0.011832 |
Golgi | 0.062861 | 0.127731* | 0.047374 | 0.065576 | 0.214320* | 0.098605 | 0.029263 | 0.078018 | 0.118599* | 0.110742* | 0.030594 | 0.073277 |
Lipid Particles | 0.134683* | 0.091657 | 0.134046* | 0.316701* | 0.048408 | 0.063171 | 0.089783 | 0.246766* | 0.102933* | 0.075041 | 0.093038 | 0.273412* |
Mitochondria | 0.001892 | 0.001245 | 0.000291 | 0.001585 | 0.006307 | 0.001006 | 0.000355 | 0.002087 | 0.003517 | 0.001106 | 0.000351 | 0.001896 |
Mitotic Spindle | 0.002209 | 0.001786 | 0.003801 | 0.005194 | 0.000530 | 0.000416 | 0.000290 | 0.001383 | 0.001592 | 0.000987 | 0.000548 | 0.002835 |
None | 0.003501 | 0.003148 | 0.000173 | 0.001248 | 0.002705 | 0.003105 | 0.001039 | 0.001526 | 0.003208 | 0.003123 | 0.000975 | 0.001420 |
Nuclear Periphery | 0.005792 | 0.005840 | 0.056011 | 0.002133 | 0.003273 | 0.004909 | 0.006439 | 0.003513 | 0.004865 | 0.005297 | 0.010084 | 0.002987 |
Nuclear Periphery Foci | 0.037800 | 0.041561 | 0.138464 | 0.019026 | 0.011823 | 0.029368 | 0.012849 | 0.015171 | 0.028240 | 0.034449 | 0.022085 | 0.016639 |
Nucleolus | 0.000069 | 0.000100 | 0.000057 | 0.000148 | 0.000420 | 0.000047 | 0.000019 | 0.000119 | 0.000198 | 0.000069 | 0.000022 | 0.000130 |
Nucleus | 0.000785 | 0.002237 | 0.000844 | 0.003080 | 0.008252 | 0.001557 | 0.000505 | 0.001250 | 0.003533 | 0.001840 | 0.000530 | 0.001947 |
Peroxisomes | 0.000235 | 0.000089 | 0.000014 | 0.000671 | 0.000151 | 0.000099 | 0.000665 | 0.002063 | 0.000204 | 0.000095 | 0.000617 | 0.001533 |
Vacuole | 0.143516 | 0.077051 | 0.292062* | 0.201227* | 0.035460 | 0.058991 | 0.056091 | 0.089460 | 0.103750 | 0.066516 | 0.073442 | 0.132043 |
Vacuole Periphery | 0.023222 | 0.035012 | 0.032354 | 0.029172 | 0.067437 | 0.028811 | 0.009309 | 0.024827 | 0.039494 | 0.031395 | 0.011003 | 0.026483 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.37 | 2.80 | -1.73 | -1.28 | -4.69 | 4.11 | 3.64 | 1.05 | -3.81 | -3.14 | 2.21 | 3.54 | -0.62 | -3.18 | -4.60 |
Bud Neck | 0.28 | -6.19 | -7.79 | -6.93 | 5.08 | -0.96 | -1.05 | -4.15 | -2.76 | -1.62 | 1.00 | -3.03 | -6.86 | -7.02 | -1.01 |
Bud Site | 0.71 | 3.70 | -10.10 | -10.09 | -11.87 | 1.46 | 3.97 | -5.45 | -7.06 | -9.65 | 2.42 | 8.19 | -10.17 | -11.95 | -16.30 |
Cell Periphery | 7.99 | -1.90 | -14.40 | -17.69 | -8.73 | -0.73 | -11.32 | -24.86 | -25.36* | -17.31 | 6.55 | -7.79 | -26.91 | -30.83* | -19.98 |
Cytoplasm | 5.36 | 5.78 | -11.57 | -14.38 | -14.27 | -2.36 | -3.46 | -14.30 | -13.22 | -13.30 | 5.74 | 7.65 | -15.12 | -19.19 | -20.41 |
Cytoplasmic Foci | -2.20 | 5.61 | -4.61 | -2.07 | -8.12 | -0.44 | 7.85 | -6.53 | -5.90 | -11.44 | -1.79 | 10.95 | -8.03 | -5.96 | -14.46 |
Eisosomes | -1.60 | -1.22 | -0.17 | 1.54 | 1.20 | 3.84 | 2.60 | 2.29 | -3.33 | -0.80 | 0.38 | 0.11 | 0.92 | 0.17 | 0.67 |
Endoplasmic Reticulum | -4.50 | 12.40 | 42.34* | 37.87* | 5.89 | -7.70 | -15.53 | 18.54 | 28.28 | 40.87* | -10.89 | -20.78 | 32.41 | 41.77* | 52.33* |
Endosome | -3.66 | 2.84 | 11.17 | 12.98 | 4.12 | -5.04 | 13.18 | 5.90 | 11.73 | -10.76 | -5.25 | 20.03 | 12.71 | 17.39 | -8.24 |
Golgi | -11.17 | 2.88 | -0.09 | 10.43 | -2.77 | 14.76 | 27.23 | 19.13 | 4.33 | -17.23 | 1.69 | 26.09 | 12.52 | 10.06 | -17.90 |
Lipid Particles | 6.90 | -0.29 | -17.78 | -22.08 | -10.14 | -4.08 | -10.21 | -32.06* | -28.27* | -23.07* | 7.07 | 1.85 | -29.26 | -35.26* | -29.33* |
Mitochondria | 1.63 | 5.77 | 0.60 | -0.54 | -2.60 | 3.44 | 3.93 | 2.76 | -2.42 | -5.42 | 3.89 | 5.42 | 2.58 | -2.20 | -5.58 |
Mitotic Spindle | 1.02 | -0.90 | -2.71 | -3.42 | -0.51 | 1.61 | 2.25 | -3.85 | -4.79 | -5.08 | 2.14 | 3.29 | -2.20 | -4.41 | -5.46 |
None | 0.30 | 4.81 | 2.98 | 2.21 | -5.15 | -0.32 | 2.41 | 1.69 | 1.80 | -2.34 | 0.12 | 4.29 | 3.37 | 2.72 | -2.58 |
Nuclear Periphery | 0.07 | -7.38 | 10.17 | 8.63 | 7.98 | -4.05 | -7.09 | -0.59 | 4.40 | 7.66 | -1.07 | -7.49 | 7.45 | 8.92 | 10.93 |
Nuclear Periphery Foci | -0.60 | -7.12 | 9.11 | 7.51 | 8.65 | -8.11 | -1.26 | -2.45 | 7.11 | -1.30 | -2.38 | 3.30 | 8.77 | 10.41 | 3.93 |
Nucleolus | -0.58 | 1.03 | -2.68 | -1.37 | -4.15 | 2.65 | 2.86 | 2.00 | -1.29 | -1.82 | 2.43 | 3.44 | 1.11 | -1.68 | -3.21 |
Nucleus | -1.45 | -0.16 | -6.90 | -2.15 | -6.59 | 2.97 | 3.73 | 3.35 | 0.34 | -3.53 | 1.69 | 3.88 | 1.22 | -1.05 | -7.37 |
Peroxisomes | 2.86 | 4.62 | -2.63 | -3.68 | -4.21 | 0.73 | -3.07 | -5.35 | -5.63 | -3.73 | 2.32 | -2.94 | -5.82 | -6.37 | -3.48 |
Vacuole | 12.69 | -7.62 | -7.53 | -17.66 | 4.47 | -6.77 | -6.82 | -14.93 | -7.95 | -8.53 | 11.08 | 7.89 | -6.20 | -17.51 | -13.83 |
Vacuole Periphery | -4.94 | -1.88 | -2.26 | 2.56 | 0.68 | 10.23 | 17.17 | 12.25 | 2.07 | -13.92 | 4.09 | 18.79 | 8.08 | 3.36 | -13.97 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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