Standard name
Human Ortholog
Description Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0.08 0.07 0.08 – 0 0.06 0.07 0 0.1 0 0.05 0.05
Bud 0 0 0 0 0 0 0 0 – 0 0.08 0.06 0.05 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0.07 0.08 0.1 0.17 0.29 0.24 – 0.17 0.17 0.07 0.13 0.18 0.15 0.13 0.15
Cytoplasm 0.96 0.96 0.96 0.97 0.95 0.92 0.9 0.87 – 0.79 0.72 0.7 0.86 0.81 0.84 – 0.89 0.85 0.66 0.63 0.38 0.62 0.6 0.58
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.07 0.05 0.07 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.13 0.16 – 0.27 0.35 0.35 0 0 0 – 0 0 0.09 0.06 0.13 0.08 0.08 0.07
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 4 2 2 0 1 0 – 9 6 29 30 41 52 – 11 16 4 10 68 12 39 47
Bud 0 8 1 6 10 18 14 25 – 40 55 62 19 15 8 – 7 10 0 3 19 2 23 32
Bud Neck 0 3 11 7 15 12 17 5 – 3 5 3 7 9 21 – 6 6 0 8 30 9 23 30
Bud Site 0 0 0 0 2 2 1 0 – 2 4 3 0 0 1 – 0 1 – – – – – –
Cell Periphery 0 10 29 23 23 21 23 24 – 61 58 99 67 162 162 – 78 49 4 35 122 42 103 140
Cytoplasm 53 260 661 550 846 640 907 910 – 710 531 713 344 447 571 – 420 243 47 171 250 178 477 548
Endoplasmic Reticulum 0 2 6 5 0 0 2 5 – 3 3 6 5 12 7 – 35 13 4 3 33 4 14 11
Endosome 0 0 1 0 7 1 3 3 – 2 0 1 0 1 0 – 0 0 0 1 9 1 4 5
Golgi 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 4 4 0 1 1
Mitochondria 2 8 10 10 29 73 129 172 – 246 254 359 0 0 0 – 6 3 6 16 86 23 65 64
Nucleus 0 0 0 2 3 2 5 7 – 3 5 2 1 2 2 – 3 1 0 0 0 0 0 2
Nuclear Periphery 0 0 0 0 1 0 2 0 – 5 1 12 0 0 0 – 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 1 2 0 2 6 – 1 0 2 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 2 1 2 1 – 1 0 1 0 0 0 – 1 0 0 0 1 0 0 0
SpindlePole 0 0 7 3 11 6 17 19 – 23 13 21 9 3 5 – 8 7 0 2 5 0 6 10
Vac/Vac Membrane 0 0 2 0 4 3 5 4 – 4 8 20 1 2 5 – 2 1 1 6 11 5 9 14
Unique Cell Count 55 271 690 568 888 696 1013 1050 897 733 1020 398 553 679 470 287 72 270 667 287 794 942
Labelled Cell Count 55 294 733 610 957 779 1130 1181 1113 943 1333 483 694 834 577 350 72 270 667 287 794 942


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 3.8 4.2 4.4 4.0 3.4 3.4 3.7 – 3.5 3.7 3.7 6.6 6.8 7.2 4.6 5.1 5.9 5.4 5.3 5.6
Std Deviation (1e-4) 0.3 0.7 1.4 2.2 1.7 1.4 1.3 1.2 – 1.2 1.2 1.1 1.1 1.5 1.6 0.9 1.1 1.7 1.3 1.3 1.5
Intensity Change (Log2) – – – 0.07 -0.04 -0.31 -0.28 -0.18 – -0.24 -0.18 -0.17 0.67 0.7 0.79 0.15 0.29 0.51 0.37 0.35 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4735 2.9807 2.8714 2.3449 3.5207 3.2413 0.5554 0.1773 0.462 0.7773 1.0178 0.4618 2.8302 -0.2804 -0.377 -0.0656 -0.4317 -0.2221
Actin 0.0109 0.0051 0.0062 0.0061 0.074 0.0173 0.0197 0.0106 0.0505 0.0258 0.1014 0.0029 0.0016 0.0045 0.0029 0.0144 0.0001 0.0002
Bud 0.0387 0.0247 0.0295 0.04 0.0571 0.0153 0.0019 0.0001 0.0005 0.006 0.0018 0.0003 0.001 0 0.0001 0.0005 0 0.0001
Bud Neck 0.0044 0.003 0.0093 0.0107 0.0393 0.0344 0.0052 0.0031 0.005 0.0166 0.0057 0.006 0.0008 0.0006 0.0012 0.0008 0 0.0462
Bud Periphery 0.0309 0.0225 0.0271 0.0573 0.0495 0.034 0.0056 0.0003 0.0027 0.0178 0.0189 0.0041 0.0011 0.0001 0.0003 0.0032 0 0.0011
Bud Site 0.0544 0.2092 0.0749 0.0723 0.0393 0.0096 0.0068 0.0367 0.0153 0.0191 0.0068 0.0013 0.0114 0.0011 0.0027 0.0016 0 0.0001
Cell Periphery 0.1662 0.0633 0.1051 0.1211 0.1336 0.1207 0.1727 0.0399 0.0961 0.0442 0.219 0.1083 0.0007 0.0031 0.0253 0.042 0.0001 0.0336
Cytoplasm 0.3573 0.2734 0.3314 0.3139 0.255 0.3425 0.298 0.2816 0.4158 0.3508 0.2534 0.4681 0.0207 0.2371 0.4334 0.29 0.3377 0.3629
Cytoplasmic Foci 0.0168 0.0076 0.0063 0.0204 0.0037 0.0075 0.0347 0.0041 0.0073 0.0259 0.0131 0.0077 0.0305 0.0076 0.0083 0.0149 0.0017 0.0016
Eisosomes 0.0038 0.0014 0.0029 0.0028 0.0032 0.0012 0.0015 0.0016 0.003 0.0017 0.0011 0.0005 0.0001 0.0016 0.0002 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0146 0.0047 0.0064 0.0071 0.0047 0.0098 0.0122 0.0032 0.0032 0.0058 0.0022 0.0018 0.0091 0.0079 0.0085 0.0055 0.0011 0.0016
Endosome 0.001 0.0004 0.0006 0.0015 0.0039 0.0022 0.0302 0.0005 0.0054 0.0421 0.0085 0.0004 0.2991 0.0005 0.0055 0.0155 0.0002 0.0004
Golgi 0.001 0.0003 0.0005 0.0008 0.0033 0.0008 0.0061 0.0004 0.0137 0.0157 0.0176 0.0004 0.0377 0.001 0.001 0.0257 0 0
Lipid Particles 0.0023 0.0008 0.0009 0.0019 0.0018 0.0018 0.0167 0.0008 0.007 0.0087 0.0346 0.0019 0.0221 0.002 0.0011 0.0008 0.0001 0.0002
Mitochondria 0.0036 0.0013 0.0012 0.0026 0.0039 0.0013 0.0021 0.0001 0.0026 0.039 0.0324 0 0.01 0.0001 0.0001 0.0141 0 0
None 0.2577 0.3516 0.3406 0.274 0.2513 0.3515 0.31 0.6141 0.3577 0.3311 0.2388 0.3937 0.0099 0.7313 0.5054 0.5661 0.6583 0.5506
Nuclear Periphery 0.0015 0.0013 0.0021 0.0042 0.0131 0.0041 0.0184 0.0003 0.0002 0.0033 0.0004 0.0001 0.0293 0.0002 0.0011 0.0006 0.0001 0.0001
Nucleolus 0.0041 0.0027 0.0041 0.0096 0.004 0.0033 0.0014 0.0002 0.0001 0.0007 0.0004 0.0001 0.0061 0.0001 0.0001 0.0001 0 0
Nucleus 0.0136 0.0127 0.0204 0.0224 0.0421 0.0189 0.0179 0.0004 0.0003 0.0015 0.0004 0.0002 0.4596 0.0003 0.0006 0.0005 0.0001 0.0001
Peroxisomes 0.0006 0.0004 0.0005 0.0007 0.001 0.0008 0.0049 0.0004 0.012 0.0232 0.0381 0.0002 0.0017 0.0004 0.0003 0.001 0 0
Punctate Nuclear 0.0039 0.0098 0.0259 0.0206 0.0105 0.0149 0.0206 0.0005 0.0003 0.0007 0.0014 0.0003 0.0077 0.0003 0.0006 0.0003 0.0001 0
Vacuole 0.012 0.0037 0.0039 0.009 0.0048 0.0075 0.0111 0.0009 0.0011 0.0152 0.0036 0.0016 0.0329 0.0004 0.0013 0.0018 0.0002 0.0009
Vacuole Periphery 0.0006 0.0002 0.0003 0.0012 0.0009 0.0005 0.0022 0 0.0001 0.0051 0.0003 0 0.0071 0 0.0002 0.0003 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.1734 7.3065 6.3254 10.0661 7.0648 8.1688 11.697 13.5041 12.2544 8.5459
Translational Efficiency 0.3285 0.7118 0.8762 0.7408 0.507 0.6155 0.4606 0.489 0.7256 0.4488

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
93 1041 1533 105 78 875 179 280 171 1916 1712 385

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 968.86 620.62 803.39 922.42 572.63 679.11 798.29 798.66 788.12 647.33 802.86 832.41
Standard Deviation 148.11 134.82 92.47 125.84 105.75 80.20 115.99 93.49 236.60 116.88 95.21 117.11
Intensity Change Log 2 — -0.642578 -0.270188 -0.070864 — 0.246042 0.479310 0.479978 — -0.246114 0.055260 0.158989

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003870 0.000319 0.001879 0.000952 0.000402 0.000553 0.001236 0.000360 0.002288 0.000426 0.001811 0.000521
Bud Neck 0.015942 0.031075 0.008048 0.002023 0.015949 0.056105 0.014081 0.005921 0.015945 0.042505 0.008679 0.004858
Bud Site 0.039115 0.007857 0.016467 0.008046 0.001409 0.014224 0.047538 0.034517 0.021915 0.010765 0.019715 0.027298
Cell Periphery 0.007543 0.001460 0.005439 0.000219 0.000344 0.000567 0.001428 0.000801 0.004259 0.001052 0.005019 0.000642
Cytoplasm 0.241693* 0.469062* 0.479441* 0.460734* 0.444300* 0.376015* 0.325320* 0.397652* 0.334110* 0.426569* 0.463327* 0.414856*
Cytoplasmic Foci 0.084767 0.230156* 0.041757 0.029171 0.272294* 0.321440* 0.044134 0.050504 0.170306 0.271844* 0.042006 0.044686
Eisosomes 0.003151 0.004628 0.001234 0.000099 0.000636 0.000808 0.000401 0.000253 0.002004 0.002883 0.001147 0.000211
Endoplasmic Reticulum 0.005798 0.000770 0.008603 0.003087 0.000382 0.000654 0.010126 0.002773 0.003327 0.000717 0.008762 0.002859
Endosome 0.043123 0.011552 0.002539 0.004805 0.011227 0.031037 0.003318 0.003897 0.028574 0.020451 0.002620 0.004145
Golgi 0.003975 0.003713 0.000364 0.001337 0.005799 0.007489 0.000396 0.000434 0.004807 0.005437 0.000367 0.000681
Lipid Particles 0.003055 0.010494 0.003225 0.002689 0.012524 0.013061 0.003234 0.002562 0.007374 0.011666 0.003226 0.002596
Mitochondria 0.000122 0.005540 0.000229 0.000893 0.003141 0.012154 0.006651 0.000400 0.001499 0.008561 0.000900 0.000534
Mitotic Spindle 0.000343 0.002044 0.001078 0.034589 0.000153 0.003562 0.001498 0.003800 0.000256 0.002737 0.001122 0.012197
None 0.000222 0.031653 0.001966 0.004909 0.001455 0.002307 0.001788 0.002557 0.000785 0.018251 0.001947 0.003199
Nuclear Periphery 0.000279 0.000328 0.000680 0.000947 0.000538 0.000170 0.001001 0.001132 0.000397 0.000256 0.000714 0.001082
Nuclear Periphery Foci 0.002366 0.000640 0.002258 0.003705 0.000200 0.000191 0.002372 0.001315 0.001378 0.000435 0.002269 0.001967
Nucleolus 0.000151 0.001606 0.000189 0.000327 0.001847 0.000963 0.000266 0.000254 0.000925 0.001312 0.000197 0.000274
Nucleus 0.054600 0.118440 0.345521* 0.188827* 0.176246* 0.074110 0.388566* 0.289918* 0.110088 0.098195 0.350021* 0.262348*
Peroxisomes 0.003762 0.020454 0.000424 0.002046 0.015814 0.023971 0.001274 0.000588 0.009259 0.022060 0.000513 0.000985
Vacuole 0.475765* 0.047513 0.078556 0.250454* 0.034784 0.059095 0.144060 0.199198 0.274616* 0.052803 0.085405 0.213177*
Vacuole Periphery 0.010358 0.000696 0.000104 0.000143 0.000554 0.001523 0.001314 0.001165 0.005886 0.001074 0.000230 0.000886

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.60 0.79 1.45 -1.20 2.82 -0.46 -1.96 0.09 1.20 3.57 1.50 0.24 1.50 -0.02 3.66
Bud Neck -3.21 0.96 2.13 10.97 8.03 -6.94 0.32 2.45 12.87 2.02 -6.96 1.64 2.91 15.91 3.99
Bud Site 2.91 1.92 3.05 0.77 3.96 -6.17 -3.58 -2.90 -0.81 1.24 1.56 0.12 0.36 -1.11 0.35
Cell Periphery 1.29 0.27 1.66 5.00 5.29 -0.91 -3.08 -0.84 0.23 2.05 1.23 -0.58 1.57 2.91 5.06
Cytoplasm -8.59 -8.60 -2.32 5.17 5.11 2.08 4.50 2.98 1.99 -2.34 -4.32 -5.15 -0.14 5.98 7.15
Cytoplasmic Foci -13.79 3.98 5.97 28.64 5.76 -1.83 9.41 9.13 31.32 -1.10 -6.88 8.69 8.80 37.91 1.14
Eisosomes -1.26 0.99 1.75 4.71 5.13 -0.63 1.61 2.30 2.93 1.62 -1.33 0.78 1.97 5.10 4.81
Endoplasmic Reticulum 2.18 -1.70 0.45 -7.46 4.54 -0.90 -5.69 -9.41 -6.98 3.62 2.02 -4.36 -0.88 -9.92 5.61
Endosome 2.64 3.70 3.57 7.86 -1.96 -4.93 2.42 2.37 12.11 -0.17 0.65 4.25 4.11 13.98 -1.37
Golgi -0.38 1.63 1.56 5.71 -0.23 -0.38 1.21 1.22 8.71 0.10 -0.50 1.76 1.77 10.71 0.23
Lipid Particles -6.73 -1.14 0.45 7.12 2.02 -0.09 3.46 3.65 8.78 1.04 -2.80 3.06 3.47 11.03 1.98
Mitochondria -4.01 -2.70 -1.44 3.78 -0.74 -4.07 -0.24 1.85 7.22 1.16 -5.62 1.12 1.63 7.80 0.80
Mitotic Spindle -1.48 -0.89 -1.60 -1.40 -1.52 -2.50 -1.78 -1.91 0.30 0.55 -2.87 -1.87 -2.24 -1.17 -1.71
None -7.25 -6.33 -3.76 6.52 -1.40 -1.11 -0.62 -1.36 0.41 -0.88 -7.27 -3.92 -4.26 6.57 -1.18
Nuclear Periphery 0.53 -6.36 -7.56 -8.17 -5.02 2.25 -4.53 -5.06 -11.29 0.45 2.26 -4.83 -8.72 -12.96 -6.57
Nuclear Periphery Foci 4.32 -0.48 -3.62 -7.31 -3.76 -0.49 -6.22 -7.30 -7.02 1.73 3.88 -4.02 -3.90 -9.16 -0.17
Nucleolus -5.65 -1.17 -0.98 5.38 -0.32 0.90 1.65 1.68 4.82 0.33 -0.89 1.67 1.55 6.84 -1.72
Nucleus -4.66 -21.83 -6.35 -4.20 8.30 4.76 -6.84 -4.42 -14.18 3.46 1.26 -17.28 -8.75 -13.42 6.43
Peroxisomes -5.75 2.71 1.97 6.78 -1.02 -1.24 2.45 2.50 10.57 0.92 -3.80 2.94 2.87 11.61 -1.17
Vacuole 14.92 13.50 2.10 -12.25 -10.81 -3.91 -10.06 -14.24 -13.24 -2.50 9.62 7.50 -0.93 -17.74 -14.03
Vacuole Periphery 1.15 1.24 1.24 7.33 -0.04 -3.67 -0.38 -0.03 1.86 0.30 1.01 1.28 1.19 1.77 -0.78
Standard name
Human Ortholog
Description Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Pns1

Pns1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pns1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available