Standard name | |
---|---|
Human Ortholog | |
Description | Broad-specificity lysophospholipid acyltransferase; part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 28 | 16 | 26 | 16 | 26 | 19 | – | 19 | 7 | 16 | 8 | 17 | 12 | 7 | 10 | 37 | 22 | 19 | 8 | 10 | 12 | 6 | 7 | 7 |
Bud | 6 | 3 | 4 | 1 | 8 | 11 | – | 14 | 16 | 11 | 29 | 19 | 2 | 3 | 1 | 7 | 7 | 1 | 1 | 1 | 0 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 4 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 37 | 25 | 42 | 51 | 51 | 45 | – | 21 | 33 | 33 | 29 | 48 | 66 | 86 | 81 | 115 | 91 | 90 | 8 | 4 | 19 | 7 | 8 | 11 |
Cytoplasm | 41 | 44 | 53 | 72 | 84 | 99 | – | 170 | 146 | 117 | 131 | 137 | 51 | 57 | 94 | 90 | 80 | 46 | 6 | 14 | 7 | 2 | 3 | 3 |
Endoplasmic Reticulum | 361 | 176 | 262 | 168 | 167 | 218 | – | 187 | 73 | 144 | 109 | 128 | 243 | 250 | 266 | 3 | 3 | 3 | 427 | 201 | 324 | 128 | 164 | 128 |
Endosome | 0 | 0 | 2 | 1 | 15 | 3 | – | 11 | 7 | 3 | 2 | 4 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 3 | 1 | 1 | 0 |
Golgi | 2 | 0 | 2 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 15 | 2 | 2 | 1 | 0 | 2 | 1 |
Mitochondria | 4 | 7 | 24 | 51 | 69 | 156 | – | 169 | 132 | 178 | 205 | 277 | 0 | 0 | 0 | 25 | 28 | 13 | 6 | 3 | 7 | 5 | 7 | 5 |
Nucleus | 0 | 0 | 3 | 0 | 0 | 1 | – | 2 | 3 | 1 | 4 | 2 | 3 | 0 | 2 | 3 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 4 | 0 | 3 | 1 | – | 6 | 1 | 10 | 5 | 9 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 13 | 3 | 19 | 12 | 73 | 33 | – | 157 | 67 | 56 | 60 | 62 | 2 | 10 | 8 | 51 | 27 | 22 | 5 | 5 | 4 | 2 | 5 | 5 |
Unique Cell Count | 428 | 224 | 350 | 278 | 380 | 428 | 613 | 369 | 431 | 440 | 531 | 331 | 345 | 394 | 278 | 235 | 192 | 476 | 253 | 387 | 160 | 205 | 167 | |
Labelled Cell Count | 492 | 274 | 442 | 372 | 498 | 587 | 758 | 487 | 570 | 583 | 703 | 380 | 413 | 463 | 340 | 267 | 218 | 476 | 253 | 387 | 160 | 205 | 167 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.2 | 9.2 | 7.2 | 7.3 | 7.7 | 6.7 | – | 6.9 | 6.5 | 6.1 | 6.0 | 5.8 | 9.4 | 9.6 | 9.6 | 8.8 | 10.4 | 12.1 | 9.7 | 10.2 | 10.8 |
Std Deviation (1e-4) | 1.1 | 1.4 | 1.0 | 1.3 | 1.2 | 1.3 | – | 1.4 | 1.5 | 1.1 | 1.2 | 1.1 | 1.1 | 1.0 | 1.1 | 1.5 | 1.8 | 2.0 | 1.3 | 1.6 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.1 | -0.09 | – | -0.06 | -0.15 | -0.23 | -0.27 | -0.31 | 0.39 | 0.43 | 0.42 | 0.3 | 0.54 | 0.76 | 0.45 | 0.51 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.8 | -0.3 | -1.8 | – | -3.1 | -3.5 | -2.3 | -3.9 | -2.8 | -2.2 | -3.3 | -3.1 | 2.4 | 0.8 | 1.0 |
Bud | 0 | 1.0 | 1.4 | – | 1.3 | 2.6 | 1.4 | 3.8 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.2 | 0.6 | -0.7 | – | -5.2 | -1.3 | -2.0 | -2.6 | -1.4 | 2.8 | 4.4 | 3.1 | 8.4 | 7.6 | 9.0 |
Cytoplasm | 3.4 | 2.4 | 2.8 | – | 4.5 | 7.3 | 4.0 | 4.8 | 3.8 | 0.1 | 0.5 | 3.0 | 5.1 | 5.3 | 2.5 |
Endoplasmic Reticulum | -3.9 | -8.5 | -6.8 | – | -13.3 | -14.8 | -11.5 | -14.0 | -14.8 | -0.4 | -0.7 | -2.2 | -18.6 | -17.5 | -16.3 |
Endosome | 0 | 3.0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.6 |
Mitochondria | 4.4 | 4.6 | 9.7 | – | 7.7 | 9.4 | 10.9 | 12.2 | 13.9 | -4.9 | -5.0 | -5.3 | 1.0 | 2.1 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 1.2 | 0 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.6 | 5.6 | 1.3 | – | 7.8 | 5.3 | 3.6 | 3.8 | 3.1 | -3.6 | -1.7 | -2.5 | 5.1 | 2.7 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.0549 | 7.3828 | 7.3157 | 6.7022 | 7.383 | 7.2494 | 8.3831 | 8.3804 | 8.7721 | 8.2556 | 8.1133 | 8.6907 | 8.2346 | 7.8759 | 8.2422 | 7.7959 | 8.8305 | 8.01 |
Actin | 0.0037 | 0.009 | 0.0185 | 0.004 | 0.0739 | 0.0013 | 0.0218 | 0.0012 | 0.0105 | 0.0014 | 0.003 | 0.0029 | 0.0088 | 0.0049 | 0.005 | 0.0059 | 0.0112 | 0.0042 |
Bud | 0.0002 | 0.0019 | 0.0047 | 0.0036 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0015 | 0.0001 | 0.0004 | 0.0003 | 0.0011 | 0.0077 | 0.001 | 0.0067 | 0.0013 | 0.0009 |
Bud Neck | 0.0036 | 0.0033 | 0.0015 | 0.0022 | 0.0077 | 0.005 | 0.0006 | 0.0009 | 0.002 | 0.0008 | 0.0004 | 0.006 | 0.0007 | 0.0014 | 0.0074 | 0.0005 | 0.0015 | 0.0084 |
Bud Periphery | 0.0007 | 0.0016 | 0.0047 | 0.0031 | 0.0019 | 0.0006 | 0.0008 | 0.0018 | 0.0077 | 0.0032 | 0.0019 | 0.0019 | 0.0061 | 0.0226 | 0.0072 | 0.0333 | 0.0103 | 0.0069 |
Bud Site | 0.0065 | 0.032 | 0.0082 | 0.0053 | 0.0007 | 0.0006 | 0.0005 | 0.0073 | 0.0042 | 0.0001 | 0.0004 | 0.0001 | 0.0007 | 0.013 | 0.0006 | 0.0006 | 0.0008 | 0.0011 |
Cell Periphery | 0.0104 | 0.0091 | 0.0178 | 0.0064 | 0.0018 | 0.0043 | 0.01 | 0.0156 | 0.0127 | 0.0115 | 0.0017 | 0.0037 | 0.0035 | 0.0172 | 0.0083 | 0.0042 | 0.0031 | 0.0075 |
Cytoplasm | 0.1017 | 0.2694 | 0.1186 | 0.1307 | 0.0554 | 0.0888 | 0.0432 | 0.1388 | 0.1166 | 0.0389 | 0.0803 | 0.0831 | 0.0661 | 0.174 | 0.1123 | 0.0753 | 0.0923 | 0.1412 |
Cytoplasmic Foci | 0.0038 | 0.007 | 0.0083 | 0.0022 | 0.0044 | 0.0032 | 0.0054 | 0.0032 | 0.008 | 0.0005 | 0.0062 | 0.0029 | 0.0039 | 0.0103 | 0.0053 | 0.0198 | 0.0053 | 0.007 |
Eisosomes | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0009 | 0.0001 | 0.0036 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0024 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.7894 | 0.573 | 0.7317 | 0.7664 | 0.7037 | 0.8488 | 0.8208 | 0.7558 | 0.7922 | 0.927 | 0.7139 | 0.8653 | 0.7479 | 0.6104 | 0.7308 | 0.6592 | 0.7677 | 0.6756 |
Endosome | 0.0094 | 0.0162 | 0.0236 | 0.0041 | 0.0833 | 0.0097 | 0.0171 | 0.0073 | 0.0049 | 0.0018 | 0.0465 | 0.0041 | 0.018 | 0.0046 | 0.0118 | 0.0297 | 0.0179 | 0.0108 |
Golgi | 0.0019 | 0.0047 | 0.0066 | 0.0012 | 0.0174 | 0.002 | 0.0117 | 0.0021 | 0.006 | 0.0009 | 0.0125 | 0.0029 | 0.005 | 0.003 | 0.0031 | 0.0132 | 0.0089 | 0.0036 |
Lipid Particles | 0.0087 | 0.0064 | 0.0151 | 0.0014 | 0.0075 | 0.0082 | 0.0197 | 0.0193 | 0.0118 | 0.0062 | 0.0163 | 0.0027 | 0.0059 | 0.0077 | 0.0061 | 0.0152 | 0.0041 | 0.0033 |
Mitochondria | 0.0029 | 0.002 | 0.0011 | 0.0026 | 0.0042 | 0.0011 | 0.0031 | 0.0009 | 0.0096 | 0.0003 | 0.0185 | 0.0006 | 0.0031 | 0.0013 | 0.0011 | 0.0234 | 0.0165 | 0.0013 |
None | 0.0238 | 0.0145 | 0.0039 | 0.0076 | 0.0017 | 0.0119 | 0.0244 | 0.0284 | 0.0029 | 0.0003 | 0.0565 | 0.0158 | 0.1066 | 0.1031 | 0.0801 | 0.0823 | 0.0448 | 0.1123 |
Nuclear Periphery | 0.013 | 0.0161 | 0.0148 | 0.0126 | 0.0212 | 0.0051 | 0.0073 | 0.0069 | 0.0036 | 0.0036 | 0.0176 | 0.0048 | 0.01 | 0.0059 | 0.006 | 0.0045 | 0.0068 | 0.0034 |
Nucleolus | 0.0003 | 0.001 | 0.0003 | 0.0013 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0005 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0003 |
Nucleus | 0.0055 | 0.0089 | 0.0032 | 0.0193 | 0.0019 | 0.0015 | 0.0007 | 0.0015 | 0.0007 | 0.0003 | 0.0106 | 0.0005 | 0.0015 | 0.002 | 0.0013 | 0.0013 | 0.0012 | 0.0014 |
Peroxisomes | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0019 | 0.0001 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0009 | 0.0003 | 0.0003 | 0.0134 | 0.0005 | 0.0029 |
Punctate Nuclear | 0.0032 | 0.0118 | 0.002 | 0.017 | 0.0013 | 0.0002 | 0.0017 | 0.0006 | 0.0003 | 0 | 0.0049 | 0.0001 | 0.0005 | 0.0004 | 0.0038 | 0.0007 | 0.0003 | 0.0016 |
Vacuole | 0.0096 | 0.0109 | 0.0139 | 0.008 | 0.0073 | 0.0068 | 0.0048 | 0.0072 | 0.0037 | 0.0026 | 0.0051 | 0.0017 | 0.0074 | 0.0086 | 0.0068 | 0.0062 | 0.0043 | 0.0056 |
Vacuole Periphery | 0.0009 | 0.0009 | 0.0011 | 0.0009 | 0.0025 | 0.0005 | 0.0007 | 0.0003 | 0.0002 | 0.0002 | 0.002 | 0.0003 | 0.0018 | 0.0011 | 0.0011 | 0.0019 | 0.0011 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 57.8397 | 32.3489 | 19.2821 | 30.1391 | 35.3807 | 36.7598 | 37.5337 | 39.4467 | 32.8698 | 42.1361 |
Translational Efficiency | 0.6315 | 0.841 | 0.9949 | 0.6079 | 0.68 | 0.9214 | 0.7658 | 0.5008 | 0.5983 | 0.6386 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
109 | 1340 | 583 | 1447 | 2516 | 1574 | 950 | 1456 | 2625 | 2914 | 1533 | 2903 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1017.18 | 963.66 | 1198.60 | 1186.10 | 900.17 | 993.22 | 1187.66 | 1212.69 | 905.03 | 979.63 | 1191.82 | 1199.44 |
Standard Deviation | 131.93 | 150.08 | 151.90 | 171.83 | 116.10 | 147.49 | 161.98 | 179.95 | 119.11 | 149.42 | 158.31 | 176.46 |
Intensity Change Log 2 | — | -0.077979 | 0.236775 | 0.221651 | — | 0.141916 | 0.399852 | 0.429941 | — | 0.029442 | 0.315638 | 0.323193 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.010374 | 0.002456 | 0.005956 | 0.014399 | 0.001162 | 0.002758 | 0.007537 | 0.009628 | 0.001545 | 0.002619 | 0.006935 | 0.012006 |
Bud Neck | 0.019691 | 0.002964 | 0.006338 | 0.009199 | 0.001403 | 0.003483 | 0.007248 | 0.006442 | 0.002163 | 0.003244 | 0.006902 | 0.007816 |
Bud Site | 0.041653 | 0.020222 | 0.027897 | 0.062782 | 0.006910 | 0.014901 | 0.030795 | 0.039213 | 0.008353 | 0.017348 | 0.029693 | 0.050961 |
Cell Periphery | 0.003394 | 0.011288 | 0.014447 | 0.044895 | 0.010488 | 0.012645 | 0.008088 | 0.051588 | 0.010194 | 0.012021 | 0.010506 | 0.048252 |
Cytoplasm | 0.142529* | 0.099523 | 0.056151 | 0.102989* | 0.153972* | 0.105636* | 0.092179 | 0.068730 | 0.153497* | 0.102825* | 0.078478 | 0.085806 |
Cytoplasmic Foci | 0.098260 | 0.035017 | 0.009448 | 0.019987 | 0.015883 | 0.018667 | 0.007491 | 0.010781 | 0.019303 | 0.026185 | 0.008235 | 0.015370 |
Eisosomes | 0.001379 | 0.001039 | 0.000984 | 0.002876 | 0.001576 | 0.001533 | 0.001139 | 0.003235 | 0.001568 | 0.001306 | 0.001080 | 0.003056 |
Endoplasmic Reticulum | 0.171843* | 0.746057* | 0.789701* | 0.580919* | 0.729151* | 0.764475* | 0.764490* | 0.704765* | 0.706009* | 0.756006* | 0.774078* | 0.643034* |
Endosome | 0.027665 | 0.009445 | 0.008679 | 0.010777 | 0.003153 | 0.006040 | 0.007900 | 0.007669 | 0.004171 | 0.007606 | 0.008197 | 0.009219 |
Golgi | 0.011914 | 0.016390 | 0.002737 | 0.008322 | 0.009875 | 0.015929 | 0.004074 | 0.008097 | 0.009959 | 0.016141 | 0.003566 | 0.008209 |
Lipid Particles | 0.030626 | 0.017967 | 0.021545 | 0.063001 | 0.015092 | 0.017221 | 0.020472 | 0.048948 | 0.015737 | 0.017564 | 0.020880 | 0.055953 |
Mitochondria | 0.010943 | 0.008340 | 0.000266 | 0.002401 | 0.011762 | 0.008697 | 0.001272 | 0.003563 | 0.011728 | 0.008533 | 0.000889 | 0.002984 |
Mitotic Spindle | 0.004103 | 0.000535 | 0.000264 | 0.006295 | 0.000110 | 0.000356 | 0.000616 | 0.001198 | 0.000276 | 0.000438 | 0.000482 | 0.003739 |
None | 0.000071 | 0.003576 | 0.000173 | 0.000949 | 0.010749 | 0.003545 | 0.002591 | 0.001164 | 0.010306 | 0.003559 | 0.001671 | 0.001057 |
Nuclear Periphery | 0.001267 | 0.000333 | 0.000687 | 0.000853 | 0.000233 | 0.000637 | 0.000792 | 0.000353 | 0.000276 | 0.000497 | 0.000752 | 0.000602 |
Nuclear Periphery Foci | 0.014809 | 0.003424 | 0.019139 | 0.009965 | 0.000956 | 0.001978 | 0.011336 | 0.002276 | 0.001531 | 0.002643 | 0.014304 | 0.006108 |
Nucleolus | 0.000162 | 0.000150 | 0.000027 | 0.000102 | 0.000078 | 0.000155 | 0.000050 | 0.000065 | 0.000081 | 0.000153 | 0.000041 | 0.000083 |
Nucleus | 0.028370 | 0.002728 | 0.004438 | 0.007389 | 0.002884 | 0.002763 | 0.008183 | 0.003512 | 0.003943 | 0.002747 | 0.006759 | 0.005445 |
Peroxisomes | 0.002982 | 0.007254 | 0.000491 | 0.006913 | 0.020400 | 0.011496 | 0.000904 | 0.012942 | 0.019676 | 0.009546 | 0.000747 | 0.009937 |
Vacuole | 0.374812* | 0.007892 | 0.030442 | 0.043581 | 0.002600 | 0.004688 | 0.022113 | 0.013557 | 0.018056 | 0.006161 | 0.025280 | 0.028522 |
Vacuole Periphery | 0.003153 | 0.003401 | 0.000191 | 0.001406 | 0.001563 | 0.002396 | 0.000732 | 0.002273 | 0.001629 | 0.002859 | 0.000526 | 0.001841 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.65 | 0.74 | -1.22 | -8.23 | -4.74 | -2.69 | -5.37 | -6.93 | -5.37 | -1.25 | -2.37 | -6.19 | -10.76 | -9.67 | -4.07 |
Bud Neck | 1.89 | 1.44 | 1.06 | -7.24 | -2.59 | -3.72 | -5.00 | -7.28 | -3.54 | 0.70 | -2.12 | -5.46 | -9.72 | -7.68 | -0.99 |
Bud Site | 1.88 | 1.01 | -2.34 | -9.36 | -5.94 | -4.29 | -6.83 | -9.77 | -6.77 | -1.68 | -5.69 | -7.68 | -15.60 | -11.61 | -5.63 |
Cell Periphery | -6.04 | -4.59 | -14.61 | -10.96 | -8.10 | -1.73 | 2.38 | -14.74 | -13.27 | -15.31 | -1.86 | -0.26 | -18.70 | -17.11 | -17.01 |
Cytoplasm | 2.62 | 4.89 | 2.25 | -1.07 | -7.36 | 9.37 | 9.55 | 17.41 | 7.10 | 3.87 | 11.64 | 14.55 | 14.89 | 3.86 | -1.65 |
Cytoplasmic Foci | 6.07 | 8.73 | 7.57 | 4.64 | -5.35 | -1.57 | 5.75 | 3.18 | 4.20 | -2.01 | -3.88 | 7.62 | 2.10 | 5.93 | -5.88 |
Eisosomes | 0.83 | 0.91 | -4.83 | -6.59 | -6.12 | 0.17 | 1.86 | -4.31 | -4.33 | -5.55 | 1.26 | 2.34 | -5.46 | -7.15 | -8.08 |
Endoplasmic Reticulum | -21.35* | -21.62 | -14.69 | 13.75 | 13.71 | -3.81 | -2.59 | 2.48 | 5.32 | 4.15 | -6.30 | -6.52 | 7.81 | 13.81 | 12.90 |
Endosome | 3.59 | 3.73 | 3.18 | -1.69 | -2.20 | -5.11 | -6.53 | -5.66 | -1.77 | 0.36 | -6.96 | -7.16 | -8.86 | -2.65 | -1.41 |
Golgi | -1.36 | 1.57 | 0.38 | 4.82 | -4.60 | -4.13 | 6.88 | 1.39 | 4.40 | -3.06 | -5.70 | 9.00 | 1.79 | 6.47 | -5.04 |
Lipid Particles | 1.84 | 0.91 | -6.01 | -12.47 | -9.33 | -1.67 | -2.53 | -11.15 | -10.13 | -7.55 | -1.76 | -3.10 | -17.21 | -16.08 | -12.18 |
Mitochondria | 0.13 | 1.65 | 1.26 | 5.44 | -8.56 | 2.53 | 14.05 | 8.82 | 4.60 | -3.84 | 3.03 | 14.53 | 10.88 | 7.14 | -6.33 |
Mitotic Spindle | 1.06 | 1.16 | -1.13 | -4.78 | -5.05 | -1.34 | -2.17 | -2.04 | -1.48 | -1.01 | -1.06 | -1.29 | -5.28 | -4.97 | -4.90 |
None | -3.85 | -2.02 | -2.10 | 2.63 | -1.83 | 5.62 | 5.93 | 8.84 | 3.04 | 1.50 | 5.81 | 7.52 | 8.92 | 3.99 | 0.99 |
Nuclear Periphery | 2.12 | 1.16 | 0.57 | -3.71 | -1.33 | -1.38 | -3.83 | -2.68 | 0.98 | 3.12 | -1.35 | -4.63 | -4.70 | -0.77 | 1.11 |
Nuclear Periphery Foci | 5.09 | -1.82 | 1.40 | -5.48 | 3.38 | -2.65 | -6.70 | -5.17 | -0.77 | 5.81 | -2.59 | -9.92 | -8.56 | -5.60 | 5.59 |
Nucleolus | -0.09 | 3.44 | 1.27 | 0.63 | -5.78 | -0.98 | 2.37 | 0.96 | 1.10 | -1.05 | -1.32 | 4.54 | -0.64 | 1.22 | -4.34 |
Nucleus | 4.20 | 3.86 | 3.24 | -6.56 | -3.39 | 0.31 | -6.04 | -1.30 | -1.39 | 4.57 | 3.05 | -4.42 | -3.57 | -6.11 | 1.33 |
Peroxisomes | -5.02 | 4.77 | -5.07 | 0.48 | -9.97 | 6.04 | 17.10 | 4.89 | -1.01 | -11.59 | 8.09 | 17.32 | 7.87 | -0.36 | -14.91 |
Vacuole | 14.05 | 13.13 | 12.51 | -12.56 | -3.80 | -3.66 | -9.82 | -10.08 | -7.53 | 4.10 | 6.55 | -2.50 | -4.59 | -14.20 | -2.07 |
Vacuole Periphery | -0.42 | 1.36 | 0.68 | 3.94 | -3.56 | -1.58 | 2.76 | -1.43 | 0.19 | -2.85 | -3.44 | 4.71 | -0.68 | 2.36 | -3.93 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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