Standard name
Human Ortholog
Description Actin assembly factor; C-terminal WCA domain activates Arp2/3 complex-mediated nucleation of branched actin filaments, polyproline domain nucleates actin filaments independent of Arp2/3; mutants are defective in endocytosis, bud site selection, cytokinesis; human homolog WAS (Wiskott-Aldrich Syndrome) implicated in severe immunodeficiency; human WAS complements yeast null mutant, but only in presence of WIPF1, which mediates localization of WAS to cortical patches
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.68 0.72 – – 0.68 0.49 0.61 0.62 0.4 0.41 0.35 0.4 0.7 0.74 0.61 0.51 0.52 0.44 0.78 0.69 0.56 0.69 0.67 0.7
Bud 0.18 0.18 – – 0.32 0.16 0.15 0.21 0.17 0.14 0.15 0.15 0.14 0.15 0.13 0.16 0.06 0.09 0.05 0.1 0.06 0.06 0.06 0.05
Bud Neck 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.09 0.08 0.08
Bud Site 0.12 0.09 – – 0 0.12 0 0 0 0.06 0 0 0.09 0 0 0 0 0 – – – – – –
Cell Periphery 0.09 0.17 – – 0.05 0 0.07 0.13 0.08 0.1 0 0 0.15 0.18 0.23 0.11 0.15 0.09 0 0 0 0 0 0
Cytoplasm 0 0.09 – – 0.11 0.07 0.07 0.07 0 0 0 0.06 0.14 0.07 0.1 0.13 0.1 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.07 – – 0.05 0.12 0.13 0.17 0.05 0.11 0.09 0 0.09 0.1 0.07 0 0 0 0 0 0 0 0 0
Endosome 0 0 – – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.05 – – 0.16 0.07 0.09 0.09 0.1 0.12 0 0.11 0 0.09 0.08 0.18 0.29 0.2 0 0 0 0 0 0
Mitochondria 0.49 0.29 – – 0 0.32 0.26 0.22 0.48 0.52 0.8 0.63 0.23 0.05 0 0.13 0.1 0.07 0 0 0.05 0 0 0
Nucleus 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.36 0.23 – – 0.16 0.29 0.22 0.18 0.24 0.22 0.14 0.16 0.2 0.34 0.34 0.24 0.38 0.22 0.05 0 0.1 0.05 0.05 0
SpindlePole 0 0 – – 0.05 0 0 0.07 0.05 0 0 0 0 0 0.05 0.09 0.1 0.18 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 124 101 – – 13 51 84 92 57 43 39 53 57 153 127 23 25 40 158 116 22 99 170 180
Bud 32 25 – – 6 17 21 31 24 15 16 20 11 32 28 7 3 8 10 16 2 8 16 13
Bud Neck 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 13 0 13 20 19
Bud Site 21 13 – – 0 12 5 6 5 6 4 5 7 7 2 1 1 2 – – – – – –
Cell Periphery 16 24 – – 1 4 10 19 12 11 4 5 12 37 48 5 7 8 3 2 0 0 2 5
Cytoplasm 7 12 – – 2 7 10 10 3 3 4 8 11 14 21 6 5 11 1 0 1 2 2 4
Endoplasmic Reticulum 5 10 – – 1 13 18 25 7 12 10 4 7 20 15 1 1 4 0 0 1 0 2 1
Endosome 1 0 – – 1 1 1 4 3 2 0 1 0 2 1 1 0 3 5 6 1 1 7 8
Golgi 4 7 – – 3 7 13 14 14 13 4 15 1 19 17 8 14 18 0 1 1 0 2 1
Mitochondria 89 41 – – 0 33 36 33 68 55 88 82 19 10 7 6 5 6 3 1 1 3 7 4
Nucleus 0 1 – – 0 1 1 1 0 1 1 1 0 1 2 1 0 1 0 1 0 0 0 0
Nuclear Periphery 2 0 – – 0 0 0 0 2 1 2 0 0 0 0 0 1 0 0 0 1 0 0 0
Nucleolus 2 0 – – 1 1 1 0 0 0 2 1 1 0 0 1 0 2 0 0 0 0 0 0
Peroxisomes 66 32 – – 3 30 30 27 34 23 15 21 16 71 70 11 18 20 9 4 4 6 12 10
SpindlePole 3 2 – – 1 3 4 10 7 3 1 5 2 9 10 4 5 16 0 0 1 0 0 0
Vac/Vac Membrane 1 0 – – 1 3 3 3 4 4 0 1 0 1 0 0 1 4 1 0 0 2 6 2
Unique Cell Count 182 140 19 104 137 149 142 106 110 131 81 207 208 45 48 90 204 169 40 143 256 257
Labelled Cell Count 373 268 33 183 237 275 240 192 190 222 144 376 348 75 86 143 204 169 40 143 256 257


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 8.8 – – 10.1 8.7 8.9 8.8 7.4 7.9 6.9 7.7 8.3 8.5 8.5 10.7 9.8 10.6 8.0 8.3 8.5
Std Deviation (1e-4) 1.4 2.2 – – 2.0 2.6 2.3 1.9 1.8 2.1 1.8 2.1 1.7 1.7 1.7 3.0 2.5 3.4 1.5 1.4 1.4
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3038 3.5225 2.8834 3.3591 2.2393 3.3846 3.3876 4.0146 2.946 2.4512 2.8675 3.2896 4.9502 3.8018 3.9478 4.3135 3.7675 3.8978
Actin 0.7204 0.6878 0.8833 0.742 0.6041 0.8814 0.784 0.5966 0.7431 0.7687 0.8884 0.8021 0.873 0.6901 0.8991 0.9561 0.9368 0.8843
Bud 0.0007 0.0014 0.0063 0.0067 0.0097 0.0015 0.001 0.0026 0.0159 0.0131 0.0022 0.0018 0.0028 0.0056 0.0087 0.0043 0.0022 0.0011
Bud Neck 0.022 0.0099 0.0125 0.0383 0.004 0.0546 0.0117 0.0115 0.0341 0.0039 0.0008 0.1104 0.0122 0.0097 0.0184 0.0063 0.0459 0.0844
Bud Periphery 0.001 0.0016 0.0128 0.0223 0.0275 0.0071 0.0015 0.0044 0.0189 0.0377 0.005 0.0133 0.0086 0.0056 0.0194 0.0111 0.0072 0.0054
Bud Site 0.0328 0.276 0.0401 0.0034 0.0085 0.002 0.0362 0.3305 0.0944 0.0119 0.0011 0.0039 0.0365 0.2319 0.0417 0.0061 0.0023 0.0023
Cell Periphery 0.0027 0.0033 0.002 0.0006 0.0028 0.0008 0.0015 0.0017 0.0012 0.0008 0.0001 0.0016 0.002 0.0012 0.0007 0.0013 0.0005 0.0007
Cytoplasm 0.0025 0.0117 0.0038 0.0023 0.0681 0.0005 0.0038 0.0191 0.0023 0.0008 0.0008 0.0007 0.0012 0.0174 0.0082 0.0002 0.0001 0.0038
Cytoplasmic Foci 0.0328 0.0019 0.0074 0.0344 0.0189 0.0055 0.028 0.0057 0.0095 0.0219 0.0169 0.0076 0.0086 0.0126 0.0004 0.0002 0.0008 0.0014
Eisosomes 0.0036 0.0021 0.0033 0.0013 0.0006 0.0037 0.0139 0.0026 0.002 0.0013 0.0006 0.0079 0.0283 0.0021 0.0015 0.0133 0.0017 0.0091
Endoplasmic Reticulum 0.0068 0.0001 0.0003 0.0012 0.0007 0.0021 0.0014 0.0003 0.0003 0.0022 0.0002 0.0001 0.0007 0.0001 0.0001 0 0 0.0001
Endosome 0.0242 0.0001 0.0011 0.0229 0.0278 0.0098 0.0294 0.0026 0.0177 0.0119 0.0282 0.0013 0.0033 0.0008 0.0001 0 0.0001 0.0008
Golgi 0.0159 0.0006 0.0024 0.0236 0.0403 0.0066 0.0143 0.003 0.0129 0.0123 0.0026 0.0036 0.0033 0.0031 0.0003 0.0005 0.0007 0.0022
Lipid Particles 0.0508 0.0002 0.0057 0.0216 0.0251 0.0017 0.0271 0.0003 0.0126 0.0372 0.0213 0.0025 0.0049 0.0006 0 0 0.0002 0.0002
Mitochondria 0.0221 0.0002 0.0019 0.0159 0.1331 0.0024 0.0167 0.0004 0.0188 0.0133 0.0003 0.0281 0.0012 0.0006 0.0004 0.0001 0.0001 0.0004
None 0.0158 0.0001 0.0046 0.0054 0.0004 0.0003 0.0023 0.0002 0.0008 0.002 0.0005 0.0003 0.0015 0.0125 0.0002 0 0 0.0001
Nuclear Periphery 0.0031 0 0.0002 0.0071 0.0004 0.0043 0.001 0.0002 0.0002 0.0037 0.0032 0 0.0005 0 0 0 0 0
Nucleolus 0.0013 0 0.0002 0.0029 0.0002 0.0001 0.0004 0 0.0001 0.0006 0.0006 0.0001 0.0002 0 0 0 0 0
Nucleus 0.0009 0 0.0001 0.0038 0.0003 0.0003 0.0004 0.0003 0.0001 0.0007 0.0007 0.0001 0.0002 0.0001 0 0 0 0.0001
Peroxisomes 0.0323 0.0029 0.0106 0.0274 0.023 0.0139 0.0206 0.004 0.0111 0.0519 0.0037 0.0141 0.0088 0.0057 0.0007 0.0003 0.0015 0.0022
Punctate Nuclear 0.0064 0.0001 0.0007 0.015 0.0003 0.0006 0.0015 0.0001 0.0003 0.0015 0.0222 0.0001 0.0009 0.0002 0 0 0 0
Vacuole 0.0012 0.0001 0.0004 0.0011 0.0024 0.0004 0.0021 0.0138 0.0032 0.0014 0.0004 0.0002 0.0012 0.0001 0.0001 0 0 0.0013
Vacuole Periphery 0.0009 0 0.0001 0.0008 0.0017 0.0004 0.0009 0.0003 0.0006 0.001 0.0002 0.0002 0.0003 0 0 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.9231 10.372 15.7778 18.2679 15.7954 13.9154 20.8431 19.6417 19.0277 16.6858
Translational Efficiency 1.9913 2.5065 1.5799 1.2949 1.6442 2.401 1.4289 1.6195 1.8093 1.606

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1689 1566 2398 192 1754 2291 539 167 3443 3857 2937 359

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 780.60 897.61 1330.29 1196.44 926.35 969.58 1167.10 1048.43 854.85 940.36 1300.34 1127.59
Standard Deviation 124.70 171.27 202.45 149.25 122.28 153.32 180.31 142.27 143.37 164.69 208.37 163.64
Intensity Change Log 2 — 0.201505 0.769085 0.616093 — 0.065803 0.333299 0.178601 — 0.129448 0.549000 0.395211

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.792809* 0.876022* 0.902602* 0.745244* 0.808148* 0.848694* 0.602782* 0.745116* 0.800623* 0.859789* 0.847579* 0.745184*
Bud Neck 0.007549 0.002941 0.006182 0.011335 0.011362 0.005770 0.017983 0.018396 0.009491 0.004621 0.008348 0.014619
Bud Site 0.055062 0.021636 0.047045 0.130434* 0.058596 0.035751 0.112457* 0.103442* 0.056862 0.030020 0.059050 0.117878*
Cell Periphery 0.038059 0.008431 0.006953 0.034441 0.048959 0.032595 0.011890 0.011197 0.043612 0.022784 0.007859 0.023628
Cytoplasm 0.005989 0.003665 0.002224 0.002535 0.007190 0.005500 0.008021 0.002326 0.006601 0.004755 0.003287 0.002438
Cytoplasmic Foci 0.022916 0.007675 0.000692 0.005767 0.012212 0.006294 0.028792 0.012585 0.017463 0.006855 0.005849 0.008939
Eisosomes 0.020670 0.011722 0.027810 0.026154 0.035388 0.024186 0.009115 0.012096 0.028168 0.019126 0.024379 0.019614
Endoplasmic Reticulum 0.000549 0.000939 0.000712 0.004413 0.000280 0.000493 0.008199 0.002742 0.000412 0.000674 0.002086 0.003636
Endosome 0.002955 0.011514 0.000995 0.009216 0.000697 0.004996 0.032813 0.015646 0.001804 0.007642 0.006834 0.012207
Golgi 0.002696 0.011975 0.000262 0.009469 0.001211 0.005364 0.040248 0.004878 0.001939 0.008048 0.007600 0.007333
Lipid Particles 0.003388 0.007662 0.000888 0.006152 0.000409 0.002168 0.056292 0.004810 0.001871 0.004399 0.011056 0.005528
Mitochondria 0.005220 0.003392 0.000423 0.000334 0.002241 0.005171 0.001189 0.001854 0.003702 0.004448 0.000563 0.001041
Mitotic Spindle 0.000226 0.000217 0.000078 0.000204 0.000535 0.000343 0.000937 0.000444 0.000383 0.000292 0.000236 0.000316
None 0.000509 0.000139 0.000027 0.000019 0.000307 0.000101 0.000041 0.000015 0.000406 0.000116 0.000030 0.000017
Nuclear Periphery 0.000006 0.000003 0.000004 0.000006 0.000055 0.000008 0.000017 0.000007 0.000031 0.000006 0.000007 0.000006
Nuclear Periphery Foci 0.000047 0.000017 0.000009 0.000089 0.000069 0.000018 0.000358 0.000094 0.000058 0.000018 0.000073 0.000092
Nucleolus 0.000026 0.000002 0.000001 0.000001 0.000006 0.000034 0.000047 0.000001 0.000016 0.000021 0.000009 0.000001
Nucleus 0.000815 0.000037 0.000058 0.000047 0.000089 0.000375 0.002671 0.000040 0.000445 0.000238 0.000538 0.000044
Peroxisomes 0.040104 0.031788 0.002543 0.011024 0.011906 0.021590 0.056993 0.060796 0.025739 0.025731 0.012535 0.034177
Vacuole 0.000331 0.000150 0.000487 0.003098 0.000217 0.000310 0.009014 0.003493 0.000273 0.000245 0.002052 0.003282
Vacuole Periphery 0.000076 0.000071 0.000007 0.000017 0.000125 0.000239 0.000140 0.000021 0.000101 0.000171 0.000031 0.000019

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.15 -13.40 2.05 5.70 6.87 -4.91 12.10 2.59 4.22 -4.90 -9.67 -6.42 3.32 6.84 5.85
Bud Neck 4.69 1.26 -1.78 -4.21 -2.52 4.77 -2.03 -1.23 -2.25 -0.08 6.31 1.13 -1.77 -3.54 -2.16
Bud Site 7.40 1.46 -4.10 -5.99 -4.52 5.09 -5.06 -2.75 -4.21 0.44 8.40 -0.72 -4.91 -7.16 -4.64
Cell Periphery 9.96 10.91 0.50 -3.84 -4.09 4.58 10.10 9.54 6.41 0.18 8.66 16.72 4.63 -0.20 -4.06
Cytoplasm 1.99 4.06 2.28 0.69 -0.05 1.21 -0.69 3.74 3.18 3.75 2.02 3.64 4.06 2.40 1.34
Cytoplasmic Foci 6.88 11.01 5.17 0.69 -1.90 3.72 -4.68 -0.38 -1.64 2.88 7.84 7.68 2.73 -1.04 -1.19
Eisosomes 4.75 -3.00 -1.14 -3.06 0.32 4.11 9.41 5.96 3.40 -0.83 5.28 1.92 2.69 -0.15 1.44
Endoplasmic Reticulum -1.72 -1.16 -2.11 -1.89 -2.00 -1.60 -4.28 -1.61 -1.47 2.27 -2.11 -4.70 -2.66 -2.45 -1.21
Endosome -5.60 2.96 -1.96 0.67 -2.60 -5.46 -8.94 -2.41 -1.71 2.36 -7.35 -6.32 -3.10 -1.34 -1.58
Golgi -6.30 5.24 -1.84 0.64 -2.52 -4.99 -9.56 -2.81 0.34 8.29 -7.81 -6.65 -2.62 0.34 0.09
Lipid Particles -3.13 3.39 -1.04 0.54 -2.04 -4.14 -8.76 -2.62 -1.57 7.76 -3.96 -7.09 -2.30 -0.72 2.70
Mitochondria 1.92 6.73 6.81 4.73 0.75 -3.47 2.82 0.32 2.30 -0.54 -1.14 8.10 3.89 4.38 -0.82
Mitotic Spindle 0.07 3.37 0.25 0.11 -1.44 0.74 -1.18 0.34 -0.56 1.67 0.62 1.07 0.48 -0.21 -0.77
None 4.61 8.92 9.04 2.05 1.54 4.08 5.74 6.75 3.30 2.14 6.46 10.84 11.25 3.49 3.21
Nuclear Periphery 1.69 -0.36 -0.01 -1.97 0.37 0.94 0.74 0.95 0.23 2.95 0.96 0.87 0.95 -0.13 1.14
Nuclear Periphery Foci 1.38 1.33 -0.80 -1.51 -1.49 1.00 -3.47 -0.81 -1.32 1.57 1.45 -0.87 -1.05 -1.84 -0.68
Nucleolus 1.73 1.82 1.82 2.61 -0.10 -0.93 -1.83 1.32 1.06 1.98 -0.28 0.48 2.07 1.10 1.94
Nucleus 1.43 1.26 1.41 -0.86 1.67 -0.91 -1.78 2.20 1.05 1.81 0.62 -0.41 1.50 1.02 2.03
Peroxisomes 2.20 12.97 5.20 3.84 -1.75 -4.31 -7.43 -3.77 -3.01 -0.25 0.00 6.60 -1.24 -1.24 -3.19
Vacuole 1.10 -2.01 -1.13 -1.20 -0.63 -0.79 -4.00 -1.40 -1.37 1.78 0.29 -4.38 -1.79 -1.81 -0.15
Vacuole Periphery 0.16 1.71 1.46 3.80 -2.01 -0.85 -0.13 0.96 2.67 5.08 -0.91 1.19 1.40 3.11 2.10
Standard name
Human Ortholog
Description Actin assembly factor; C-terminal WCA domain activates Arp2/3 complex-mediated nucleation of branched actin filaments, polyproline domain nucleates actin filaments independent of Arp2/3; mutants are defective in endocytosis, bud site selection, cytokinesis; human homolog WAS (Wiskott-Aldrich Syndrome) implicated in severe immunodeficiency; human WAS complements yeast null mutant, but only in presence of WIPF1, which mediates localization of WAS to cortical patches
Localization
Cell Percentages actin (93%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Las17

Las17


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Las17-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available