Standard name | |
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Human Ortholog | |
Description | Mitochondrial translation elongation factor Tu (EF-Tu); involved in fundamental pathway of mtDNA homeostasis; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans; rare mutations in human mitochondrial elongation factor Tu (EFTu) associated with severe lactic acidosis, rapidly progressive fatal encephalopathy, severe infantile macrocystic leukodystrophy with micropolygyria |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | – | 6 | 2 | 3 | 0 | 2 | 3 | 4 | 4 | 0 | 1 | 3 | 11 | 18 | 17 | 35 | 16 | 0 | 2 | 2 | 1 | 1 | 4 |
Bud | 0 | – | 1 | 1 | 3 | 1 | 2 | 4 | 7 | 10 | 1 | 3 | 0 | 0 | 1 | 4 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | – | 0 | 1 | 0 | 0 | 3 | 4 | 1 | 4 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | – | 3 | 0 | 0 | 1 | 1 | 1 | 3 | 0 | 0 | 0 | 2 | 6 | 4 | 6 | 7 | 7 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 0 | – | 2 | 3 | 2 | 1 | 3 | 5 | 3 | 2 | 0 | 3 | 0 | 2 | 3 | 5 | 11 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | – | 1 | 0 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 3 | 2 | 7 | 4 | 1 | 1 | 0 | 0 | 0 | 0 |
Endosome | 0 | – | 2 | 0 | 1 | 0 | 1 | 3 | 1 | 1 | 1 | 1 | 1 | 1 | 4 | 2 | 3 | 4 | 1 | 1 | 1 | 1 | 0 | 4 |
Golgi | 3 | – | 5 | 2 | 8 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 17 | 94 | 118 | 66 | 100 | 108 | 5 | 2 | 2 | 4 | 18 | 19 |
Mitochondria | 19 | – | 110 | 154 | 137 | 130 | 296 | 264 | 254 | 457 | 283 | 285 | 53 | 102 | 141 | 8 | 9 | 21 | 33 | 29 | 111 | 30 | 71 | 84 |
Nucleus | 0 | – | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 2 | 1 | 2 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | – | 10 | 13 | 12 | 16 | 42 | 32 | 40 | 42 | 26 | 39 | 1 | 9 | 4 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3 | – | 6 | 7 | 11 | 25 | 64 | 50 | 48 | 73 | 35 | 44 | 2 | 5 | 8 | 1 | 0 | 7 | 0 | 0 | 0 | 1 | 0 | 1 |
Peroxisomes | 0 | – | 3 | 1 | 1 | 1 | 5 | 8 | 4 | 5 | 2 | 1 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 7 | 0 | 2 | 0 |
SpindlePole | 0 | – | 0 | 1 | 3 | 0 | 2 | 4 | 2 | 1 | 2 | 0 | 0 | 0 | 4 | 0 | 1 | 8 | 0 | 0 | 1 | 0 | 0 | 1 |
Vac/Vac Membrane | 3 | – | 8 | 4 | 14 | 5 | 3 | 3 | 15 | 10 | 4 | 8 | 12 | 28 | 22 | 10 | 17 | 26 | 1 | 2 | 1 | 6 | 16 | 16 |
Unique Cell Count | 20 | 125 | 161 | 157 | 153 | 329 | 306 | 289 | 500 | 299 | 319 | 67 | 155 | 206 | 88 | 144 | 165 | 48 | 42 | 131 | 47 | 116 | 140 | |
Labelled Cell Count | 28 | 157 | 189 | 198 | 182 | 426 | 385 | 385 | 613 | 357 | 392 | 91 | 262 | 332 | 121 | 195 | 222 | 48 | 42 | 131 | 47 | 116 | 140 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.8 | – | 15.4 | 13.7 | 14.1 | 13.0 | 12.7 | 12.9 | 12.9 | 12.3 | 12.4 | 12.7 | 16.0 | 16.4 | 16.2 | 19.2 | 19.1 | 19.1 | 12.1 | 18.0 | 17.7 |
Std Deviation (1e-4) | 2.7 | – | 3.2 | 3.0 | 3.0 | 2.9 | 3.0 | 3.2 | 3.2 | 3.3 | 3.9 | 3.7 | 3.3 | 3.0 | 3.3 | 4.7 | 5.4 | 5.6 | 3.1 | 4.5 | 4.4 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.13 | -0.25 | -0.29 | -0.26 | -0.26 | -0.33 | -0.32 | -0.28 | 0.05 | 0.09 | 0.07 | 0.31 | 0.31 | 0.3 | -0.35 | 0.22 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 1.3 | 3.4 | 4.4 | 1.6 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 2.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.4 | 9.9 | 9.7 | 10.8 | 11.0 | 10.6 |
Mitochondria | 2.4 | -0.2 | -0.7 | 0.6 | -0.5 | 0 | 1.2 | 2.4 | 0.4 | -1.6 | -4.3 | -4.0 | -11.4 | -13.5 | -12.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | -0.1 | 0.7 | 1.4 | 0.8 | 1.7 | 0.1 | 0.2 | 1.3 | 0 | -0.7 | -2.7 | 0 | -3.0 | -2.9 |
Nucleolus | -0.2 | 0.8 | 3.0 | 3.9 | 3.2 | 3.3 | 2.9 | 2.2 | 2.7 | 0 | 0 | -0.4 | 0 | 0 | -0.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.6 | 0.8 | -1.2 | 0 | 0 | -0.5 | 0 | 0 | 0 | 2.5 | 2.9 | 1.3 | 1.3 | 1.5 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 24.8402 | 29.8691 | 27.5917 | 26.5717 | 24.8407 | 27.3905 | 28.1724 | 30.9152 | 28.2138 | 26.4194 | 23.4436 | 28.5826 | 23.9972 | 25.9568 | 24.5994 | 21.3284 | 17.109 | 25.7476 |
Actin | 0.0438 | 0.005 | 0.0096 | 0.0015 | 0.0032 | 0.0075 | 0.0557 | 0.0203 | 0.0118 | 0.0272 | 0.0073 | 0.0027 | 0.0121 | 0.0163 | 0.012 | 0.018 | 0.0023 | 0.0006 |
Bud | 0.001 | 0.0004 | 0.0002 | 0.001 | 0.0001 | 0 | 0.0003 | 0.0006 | 0.0004 | 0.0012 | 0.002 | 0.0001 | 0.0004 | 0.0015 | 0.0002 | 0.0013 | 0.0004 | 0 |
Bud Neck | 0.0003 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0.0002 | 0.0002 | 0.0003 | 0.0009 | 0.0006 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0008 | 0.0001 | 0 |
Bud Periphery | 0.0028 | 0.0009 | 0.0006 | 0.004 | 0.0002 | 0.0001 | 0.0006 | 0.0018 | 0.0006 | 0.0027 | 0.0014 | 0.0004 | 0.0012 | 0.002 | 0.0002 | 0.003 | 0.0009 | 0 |
Bud Site | 0.0015 | 0.0004 | 0.0003 | 0.0032 | 0.0001 | 0 | 0.0008 | 0.0029 | 0.0017 | 0.0026 | 0.0592 | 0 | 0.001 | 0.0078 | 0.0039 | 0.0058 | 0.0004 | 0 |
Cell Periphery | 0.0004 | 0.0005 | 0.0002 | 0.0084 | 0.0001 | 0.0001 | 0.0006 | 0.0008 | 0.0007 | 0.0009 | 0.0003 | 0.0003 | 0.0002 | 0.0009 | 0.0002 | 0.0028 | 0.0006 | 0.0003 |
Cytoplasm | 0.0004 | 0 | 0.0001 | 0.0015 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0015 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0 |
Cytoplasmic Foci | 0.0404 | 0.0011 | 0.0015 | 0.0043 | 0.0001 | 0.0002 | 0.0045 | 0.0006 | 0.0037 | 0.0041 | 0.0294 | 0.0001 | 0.0052 | 0.0015 | 0.003 | 0.0227 | 0.0092 | 0.0001 |
Eisosomes | 0.0026 | 0.0005 | 0.0014 | 0.0007 | 0.0004 | 0.0005 | 0.0015 | 0.0014 | 0.0029 | 0.0013 | 0.0001 | 0.0006 | 0.0011 | 0.0007 | 0.001 | 0.0006 | 0.0006 | 0.0005 |
Endoplasmic Reticulum | 0.0017 | 0.0004 | 0.0002 | 0.0053 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0012 | 0.0003 | 0.0002 | 0.0001 | 0.001 | 0.0004 | 0.0012 |
Endosome | 0.0053 | 0.0046 | 0.0007 | 0.0079 | 0 | 0.0011 | 0.0023 | 0.0028 | 0.0106 | 0.0118 | 0.0086 | 0.0018 | 0.0038 | 0.0096 | 0.0051 | 0.0111 | 0.0188 | 0.0023 |
Golgi | 0.0056 | 0.0197 | 0.0027 | 0.0015 | 0.0002 | 0.0034 | 0.0062 | 0.018 | 0.0425 | 0.0207 | 0.0185 | 0.0269 | 0.0045 | 0.0484 | 0.0092 | 0.0156 | 0.0184 | 0.0391 |
Lipid Particles | 0.0296 | 0.0112 | 0.0044 | 0.0766 | 0.0006 | 0.0113 | 0.0158 | 0.0089 | 0.0066 | 0.035 | 0.0136 | 0.0008 | 0.0105 | 0.0182 | 0.0059 | 0.0415 | 0.0471 | 0.001 |
Mitochondria | 0.8208 | 0.9031 | 0.9558 | 0.7376 | 0.9926 | 0.9602 | 0.8664 | 0.8938 | 0.9038 | 0.8732 | 0.8214 | 0.9531 | 0.9419 | 0.8505 | 0.9402 | 0.8129 | 0.8434 | 0.9528 |
None | 0.0012 | 0.0001 | 0.0146 | 0.0026 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0008 | 0.0004 | 0.0002 | 0.0007 | 0.0002 | 0 |
Nuclear Periphery | 0.0043 | 0.0005 | 0.0001 | 0.0093 | 0 | 0.0008 | 0.0006 | 0.0006 | 0.0001 | 0.0009 | 0.0001 | 0.001 | 0.0027 | 0.0005 | 0.0004 | 0.0018 | 0.0004 | 0.0001 |
Nucleolus | 0.0037 | 0.0006 | 0.001 | 0.0358 | 0.0007 | 0.0001 | 0.0021 | 0.0007 | 0.0018 | 0.0006 | 0.0003 | 0.0001 | 0.0007 | 0.0026 | 0.0002 | 0.0063 | 0.0006 | 0 |
Nucleus | 0.0008 | 0.0003 | 0.0002 | 0.0155 | 0.0002 | 0.0001 | 0.0009 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0004 | 0.0011 | 0.0001 | 0.0026 | 0.0004 | 0 |
Peroxisomes | 0.0192 | 0.0017 | 0.0019 | 0.0027 | 0.0007 | 0.0014 | 0.0039 | 0.0012 | 0.003 | 0.0047 | 0.0326 | 0.0005 | 0.0031 | 0.0051 | 0.007 | 0.0228 | 0.0033 | 0.0003 |
Punctate Nuclear | 0.0047 | 0.0001 | 0.0015 | 0.0012 | 0 | 0 | 0.0074 | 0 | 0.0001 | 0.0004 | 0.0006 | 0 | 0.0008 | 0.0002 | 0.0008 | 0.0013 | 0.0001 | 0 |
Vacuole | 0.0029 | 0.0079 | 0.0008 | 0.047 | 0.0001 | 0.0013 | 0.0087 | 0.0093 | 0.0034 | 0.0029 | 0.0015 | 0.0009 | 0.0017 | 0.009 | 0.0015 | 0.0107 | 0.0068 | 0.0002 |
Vacuole Periphery | 0.007 | 0.0412 | 0.0021 | 0.0318 | 0.0008 | 0.0118 | 0.0207 | 0.0357 | 0.0053 | 0.0076 | 0.0005 | 0.0094 | 0.0073 | 0.0227 | 0.0084 | 0.0163 | 0.0453 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 162.4876 | 144.8787 | 120.8749 | 135.3057 | 130.6495 | 156.1032 | 170.3951 | 159.573 | 188.1175 | 187.5503 |
Translational Efficiency | 1.1841 | 1.1446 | 1.1533 | 1.3255 | 1.2356 | 1.4425 | 1.2364 | 1.2014 | 1.1461 | 1.108 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
93 | 226 | 990 | 1000 | 1217 | 936 | 300 | 421 | 1310 | 1162 | 1290 | 1421 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1571.52 | 1288.55 | 1767.91 | 2165.68 | 1168.43 | 1350.78 | 1878.65 | 2166.19 | 1197.05 | 1338.68 | 1793.66 | 2165.83 |
Standard Deviation | 478.11 | 250.61 | 349.50 | 430.74 | 196.76 | 230.10 | 308.80 | 514.84 | 250.82 | 235.52 | 343.67 | 457.27 |
Intensity Change Log 2 | — | -0.286412 | 0.169884 | 0.462659 | — | 0.209221 | 0.685125 | 0.890588 | — | -0.053978 | 0.412387 | 0.660840 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.015902 | 0.035786 | 0.025702 | 0.050798 | 0.005548 | 0.017582 | 0.102089* | 0.046458 | 0.006283 | 0.021123 | 0.043466 | 0.049512 |
Bud Neck | 0.003215 | 0.006281 | 0.011580 | 0.003733 | 0.003248 | 0.001904 | 0.019743 | 0.006193 | 0.003246 | 0.002755 | 0.013478 | 0.004462 |
Bud Site | 0.011722 | 0.015940 | 0.025757 | 0.010817 | 0.012864 | 0.003847 | 0.047138 | 0.018862 | 0.012783 | 0.006199 | 0.030730 | 0.013201 |
Cell Periphery | 0.000388 | 0.002697 | 0.001336 | 0.004721 | 0.001260 | 0.000743 | 0.004142 | 0.003013 | 0.001198 | 0.001123 | 0.001988 | 0.004215 |
Cytoplasm | 0.002395 | 0.001790 | 0.001944 | 0.000707 | 0.001438 | 0.000581 | 0.002051 | 0.000874 | 0.001506 | 0.000816 | 0.001969 | 0.000756 |
Cytoplasmic Foci | 0.039783 | 0.049880 | 0.038134 | 0.007154 | 0.031189 | 0.026736 | 0.035598 | 0.015333 | 0.031799 | 0.031237 | 0.037544 | 0.009577 |
Eisosomes | 0.002219 | 0.000901 | 0.000477 | 0.002029 | 0.000172 | 0.000407 | 0.001744 | 0.001379 | 0.000317 | 0.000503 | 0.000772 | 0.001836 |
Endoplasmic Reticulum | 0.010714 | 0.001308 | 0.006346 | 0.002137 | 0.002848 | 0.001346 | 0.013809 | 0.003147 | 0.003406 | 0.001339 | 0.008082 | 0.002436 |
Endosome | 0.008319 | 0.055755 | 0.058708 | 0.006283 | 0.045561 | 0.022244 | 0.048259 | 0.017896 | 0.042917 | 0.028761 | 0.056278 | 0.009724 |
Golgi | 0.058379 | 0.141739* | 0.213332* | 0.081081 | 0.078890 | 0.115688* | 0.253788* | 0.190578* | 0.077434 | 0.120755* | 0.222741* | 0.113522* |
Lipid Particles | 0.015868 | 0.017773 | 0.030316 | 0.003192 | 0.016570 | 0.007659 | 0.015891 | 0.002902 | 0.016520 | 0.009626 | 0.026961 | 0.003106 |
Mitochondria | 0.774842* | 0.603980* | 0.428434* | 0.794187* | 0.718741* | 0.766675* | 0.377959* | 0.599171* | 0.722724* | 0.735032* | 0.416696* | 0.736410* |
Mitotic Spindle | 0.001819 | 0.005988 | 0.015746 | 0.001853 | 0.004439 | 0.001787 | 0.006005 | 0.004492 | 0.004253 | 0.002604 | 0.013481 | 0.002635 |
None | 0.003644 | 0.001033 | 0.001463 | 0.001566 | 0.001329 | 0.001116 | 0.001443 | 0.002706 | 0.001493 | 0.001100 | 0.001459 | 0.001904 |
Nuclear Periphery | 0.001637 | 0.000293 | 0.004806 | 0.000274 | 0.001140 | 0.000358 | 0.001335 | 0.000753 | 0.001175 | 0.000346 | 0.003998 | 0.000416 |
Nuclear Periphery Foci | 0.004774 | 0.000906 | 0.004130 | 0.000201 | 0.001701 | 0.000359 | 0.001197 | 0.000552 | 0.001919 | 0.000465 | 0.003448 | 0.000305 |
Nucleolus | 0.003434 | 0.000409 | 0.000462 | 0.000140 | 0.000777 | 0.000135 | 0.000274 | 0.000586 | 0.000966 | 0.000188 | 0.000418 | 0.000272 |
Nucleus | 0.003129 | 0.000136 | 0.000159 | 0.000091 | 0.000724 | 0.000056 | 0.000169 | 0.000123 | 0.000895 | 0.000072 | 0.000161 | 0.000100 |
Peroxisomes | 0.019554 | 0.041529 | 0.005688 | 0.006013 | 0.008344 | 0.009675 | 0.015241 | 0.005517 | 0.009140 | 0.015871 | 0.007909 | 0.005866 |
Vacuole | 0.007438 | 0.009654 | 0.057177 | 0.006349 | 0.020777 | 0.008587 | 0.026115 | 0.027275 | 0.019830 | 0.008794 | 0.049953 | 0.012548 |
Vacuole Periphery | 0.010823 | 0.006220 | 0.068303 | 0.016675 | 0.042440 | 0.012515 | 0.026010 | 0.052192 | 0.040196 | 0.011291 | 0.058468 | 0.027198 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.27 | -1.82 | -5.55 | -1.77 | -5.02 | -4.91 | -8.45 | -5.79 | -3.93 | 4.16 | -5.89 | -10.33 | -11.53 | -6.61 | -1.21 |
Bud Neck | -2.02 | -6.86 | -0.62 | 1.75 | 7.15 | 2.41 | -6.88 | -2.91 | -4.47 | 5.34 | 0.87 | -10.08 | -2.02 | -3.03 | 8.72 |
Bud Site | -0.45 | -1.71 | 0.09 | 1.00 | 5.05 | 4.28 | -4.49 | -1.40 | -3.72 | 3.34 | 2.96 | -5.62 | -0.25 | -3.70 | 5.73 |
Cell Periphery | -1.82 | -7.00 | -4.80 | -1.35 | -3.75 | 1.95 | -3.62 | -2.58 | -3.42 | 1.17 | 0.22 | -2.89 | -4.39 | -4.33 | -3.22 |
Cytoplasm | 0.41 | 0.12 | 1.20 | 2.52 | 3.58 | 2.36 | -0.76 | 1.41 | -1.60 | 1.41 | 1.94 | -1.39 | 1.95 | 0.18 | 3.76 |
Cytoplasmic Foci | -0.93 | 0.15 | 3.39 | 8.06 | 12.38 | 1.56 | -1.06 | 5.39 | 3.86 | 4.84 | 0.21 | -2.07 | 10.07 | 10.09 | 12.88 |
Eisosomes | 3.17 | 4.46 | 0.42 | -4.17 | -6.74 | -4.64 | -4.90 | -6.16 | -4.86 | 0.98 | -3.11 | -5.06 | -8.73 | -7.56 | -5.65 |
Endoplasmic Reticulum | 1.41 | 0.64 | 1.29 | -1.82 | 4.11 | 3.06 | -3.42 | -0.40 | -2.78 | 3.30 | 3.18 | -3.88 | 1.37 | -2.90 | 5.19 |
Endosome | -7.05 | -13.43 | 0.83 | 7.80 | 17.43 | 6.99 | -0.55 | 7.52 | 1.47 | 6.51 | 4.33 | -3.74 | 11.92 | 9.15 | 18.01 |
Golgi | -4.17 | -9.45 | -1.50 | 3.98 | 13.19 | -4.52 | -10.15 | -8.18 | -5.13 | 2.99 | -5.79 | -16.94 | -5.08 | 0.87 | 11.65 |
Lipid Particles | -0.24 | -1.98 | 1.88 | 3.61 | 8.91 | 3.82 | 0.16 | 6.42 | 3.97 | 4.73 | 3.00 | -3.43 | 6.48 | 4.97 | 9.88 |
Mitochondria | 3.96 | 9.62 | -0.53 | -6.77 | -23.65* | -3.33 | 14.34 | 5.89 | 8.10 | -7.87 | -0.90 | 21.66 | -1.00 | -0.05 | -23.13* |
Mitotic Spindle | -1.09 | -6.61 | -0.05 | 1.09 | 6.87 | 1.76 | -0.75 | -0.14 | -2.11 | 0.69 | 1.08 | -4.89 | 1.40 | -0.07 | 6.65 |
None | 4.99 | 4.29 | 4.06 | -3.37 | -1.00 | 0.65 | -0.32 | -3.74 | -7.27 | -5.27 | 1.29 | 0.14 | -1.34 | -6.66 | -4.30 |
Nuclear Periphery | 1.21 | -2.55 | 1.23 | 0.28 | 8.94 | 3.49 | -0.54 | 1.81 | -2.35 | 1.79 | 3.87 | -6.43 | 3.95 | -0.63 | 8.99 |
Nuclear Periphery Foci | 0.98 | 0.16 | 1.16 | 2.80 | 8.77 | 4.17 | 1.31 | 3.55 | -1.38 | 2.55 | 3.55 | -2.95 | 4.01 | 1.54 | 8.96 |
Nucleolus | 1.65 | 1.62 | 1.80 | 1.64 | 2.96 | 1.85 | 1.44 | 0.47 | -2.18 | -1.47 | 2.23 | 1.53 | 1.98 | -1.08 | 1.41 |
Nucleus | 1.73 | 1.71 | 1.75 | 0.92 | 1.96 | 1.54 | 1.28 | 1.39 | -3.02 | 1.37 | 1.94 | 1.72 | 1.86 | -1.29 | 2.32 |
Peroxisomes | -2.14 | 1.88 | 1.82 | 4.82 | -0.20 | -0.64 | -2.14 | 1.73 | 2.06 | 3.05 | -2.89 | 0.81 | 2.03 | 4.45 | 1.40 |
Vacuole | -0.56 | -10.90 | 0.28 | 1.29 | 14.62 | 6.27 | -1.33 | -1.63 | -4.71 | -0.21 | 6.15 | -9.93 | 3.53 | -2.26 | 12.32 |
Vacuole Periphery | 1.62 | -12.91 | -1.82 | -5.32 | 13.09 | 9.60 | 4.10 | -1.64 | -7.41 | -4.43 | 10.10 | -4.65 | 3.86 | -7.08 | 8.96 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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