Standard name
Human Ortholog
Description Peripheral peroxisomal membrane protein; involved in controlling peroxisome size and number, interacts with Pex25p; PEX27 has a paralog, PEX25, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0.15 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0.05 0.05 0 0.12 0.1 0.12 0.16 0.18
Bud 0.12 0.07 0.08 0.11 0.12 0.12 0.13 0.16 0.13 0.13 0.14 0.13 0.07 0.06 0 0 0.08 0.08 0 0 0 0 0 0
Bud Neck 0 0 0.14 0.08 0.09 0.06 0 0.07 0.05 0 0 0 0.07 0 0 0 0.05 0 0 0 0 0 0 0
Bud Site 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.23 0.2 0.27 0.34 0.3 0.32 0.29 0.29 0.34 0.24 0.28 0.24 0.26 0.21 0.25 0.2 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.08 0 0 0 0.05 0 0 0 0 0 0 0 0.13 0.27 0.1 0.12 0.14 0.19 0.31 0.21 0.12 0.14 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.52 0.35 0 0.05 0.09 0.29 0.4 0.26 0.52 0.42 0.69 0.4 0 0 0 0.05 0.08 0.09 0.1 0.11 0.13 0.13 0.12 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.35 0.24 0 0.14 0.09 0.14 0.06 0.05 0.07 0 0 0.07 0.14 0.2 0.32 0.11 0.17 0.18 0.5 0.24 0.39 0.45 0.42 0.45
SpindlePole 0.24 0.21 0.62 0.52 0.43 0.36 0.3 0.42 0.24 0.28 0.19 0.32 0.56 0.51 0.46 0.58 0.56 0.47 0.1 0.1 0.1 0.09 0.08 0.08
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 13 61 12 15 6 6 18 4 8 0 10 51 18 4 4 8 14 11 44 55 84 147 176
Bud 26 15 34 45 54 66 81 87 57 62 64 65 41 43 24 18 13 22 3 3 2 7 11 12
Bud Neck 1 2 55 30 38 34 20 40 22 16 14 19 42 25 13 13 8 10 5 6 8 12 20 18
Bud Site 16 2 8 11 8 13 21 21 14 13 19 13 6 1 2 1 4 12 – – – – – –
Cell Periphery 0 2 1 1 0 0 1 2 0 1 0 0 2 0 2 2 0 0 0 0 0 1 3 3
Cytoplasm 24 51 80 109 150 161 198 164 123 161 116 138 140 190 171 103 33 53 2 7 9 16 19 14
Endoplasmic Reticulum 0 4 10 1 1 2 1 1 1 1 0 1 6 0 0 9 4 8 0 1 2 3 2 3
Endosome 8 18 4 9 15 28 10 13 6 4 0 17 26 91 224 42 21 38 50 114 110 85 127 107
Golgi 0 0 1 0 0 0 0 1 0 0 0 0 1 2 2 1 1 0 1 6 4 2 7 2
Mitochondria 116 79 6 20 40 151 244 145 225 200 325 195 13 8 3 20 14 25 26 42 68 93 105 92
Nucleus 1 1 0 2 0 3 1 3 1 5 0 1 2 1 2 3 2 4 0 0 0 0 0 0
Nuclear Periphery 0 3 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 1 0 2 1
Nucleolus 2 1 1 0 0 1 1 0 0 2 1 0 1 1 1 0 1 0 0 1 0 0 0 1
Peroxisomes 79 54 17 54 38 72 35 29 29 15 14 32 81 141 262 43 29 50 131 90 209 314 384 427
SpindlePole 53 47 251 208 188 188 183 237 104 130 91 157 330 370 374 236 95 129 26 38 55 61 70 78
Vac/Vac Membrane 2 2 2 1 4 3 2 2 4 4 6 6 0 7 5 12 1 4 2 7 2 2 3 4
Unique Cell Count 223 225 406 397 436 528 616 561 431 471 474 486 587 719 817 408 169 277 265 374 540 695 918 957
Labelled Cell Count 331 294 531 503 551 728 804 763 591 622 650 654 742 898 1089 507 234 369 265 374 540 695 918 957


Peroxisomes

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.7 5.0 4.7 4.5 3.7 3.5 4.0 3.3 3.5 3.1 3.6 5.0 5.4 5.4 6.1 5.4 5.2 4.9 5.0 5.4
Std Deviation (1e-4) 0.5 1.1 1.1 1.6 1.4 1.4 1.2 1.1 1.1 1.1 1.0 1.0 1.0 1.4 1.3 1.6 1.7 1.4 1.5 1.6 1.9
Intensity Change (Log2) – – – -0.1 -0.16 -0.42 -0.52 -0.32 -0.59 -0.51 -0.7 -0.48 -0.01 0.11 0.12 0.29 0.12 0.05 -0.02 0.01 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – –
Bud Neck – – – – – – – – – – – – 0 – –
Bud Site – – – – – – – – – – – – 0 – –
Cell Periphery – – – – – – – – – – – – 0 – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – –
Endosome – – – – – – – – – – – – 0 – –
Golgi – – – – – – – – – – – – 0 – –
Mitochondria – – – – – – – – – – – – 0 – –
Nuclear Periphery – – – – – – – – – – – – 0 – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – –
Peroxisomes – – – – – – – – – – – – 0 – –
SpindlePole – – – – – – – – – – – – 0 – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – –
Cytoplasm – – – – – – – – – – – – 0 – –
Nucleus – – – – – – – – – – – – 0 – –
Vacuole – – – – – – – – – – – – 2.7 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3793 0.6646 0.7987 0.7263 0.4115 0.8562 0.01 -0.2031 0.1194 -0.3852 0.0283 -0.0984 0.3351 -0.6218 -0.5217 -0.325 0.7386 -0.6687
Actin 0.0618 0.026 0.0431 0.0054 0.0074 0.0253 0.0402 0.0219 0.0395 0.008 0.0284 0.0149 0.0982 0.0354 0.0612 0.0025 0.0049 0.0293
Bud 0.0014 0.0061 0.0047 0.0003 0.0005 0.0026 0.0004 0.0019 0.0007 0.0001 0.0005 0.0002 0.0008 0.0132 0.0158 0.0005 0.0011 0.0008
Bud Neck 0.0032 0.0028 0.014 0.0308 0.0031 0.0064 0.0052 0.0023 0.0053 0.0006 0.0109 0.0106 0.02 0.0029 0.007 0.0331 0.0224 0.013
Bud Periphery 0.0032 0.0064 0.0072 0.001 0.001 0.0021 0.0006 0.0037 0.0019 0.0002 0.0009 0.0004 0.0011 0.0043 0.01 0.0022 0.0011 0.0011
Bud Site 0.0068 0.0223 0.0172 0.0013 0.0024 0.0015 0.0044 0.0155 0.0035 0.0005 0.0021 0.0006 0.0135 0.0479 0.0125 0.0008 0.0013 0.0015
Cell Periphery 0.0005 0.001 0.0008 0.0009 0.0001 0.001 0.0002 0.0006 0.0003 0 0.0001 0.0002 0.0003 0.0011 0.0005 0.0002 0.0008 0.0006
Cytoplasm 0.0158 0.0339 0.0196 0.0098 0.0436 0.02 0.019 0.0295 0.0169 0.005 0.0141 0.0193 0.0235 0.0681 0.0199 0.036 0.059 0.0296
Cytoplasmic Foci 0.2299 0.2315 0.1809 0.2339 0.1623 0.2617 0.3192 0.265 0.277 0.2622 0.2718 0.271 0.315 0.2826 0.2593 0.195 0.2532 0.2682
Eisosomes 0.0009 0.0011 0.001 0.0008 0.0003 0.0007 0.0007 0.001 0.0006 0.0002 0.0005 0.0007 0.0007 0.0005 0.0003 0.0006 0.0001 0.0008
Endoplasmic Reticulum 0.0066 0.0013 0.002 0.0056 0.0003 0.0011 0.0041 0.0009 0.0024 0.0001 0.0018 0.0007 0.0032 0.0021 0.0068 0.0006 0.0025 0.0038
Endosome 0.0423 0.0273 0.0319 0.0308 0.0239 0.0263 0.0302 0.0294 0.0306 0.0159 0.0361 0.0171 0.0596 0.0597 0.0258 0.0175 0.1011 0.0571
Golgi 0.018 0.0123 0.0168 0.0122 0.0109 0.0126 0.0106 0.0087 0.0121 0.0078 0.0106 0.0061 0.0281 0.0242 0.0133 0.0137 0.0611 0.0305
Lipid Particles 0.0552 0.0252 0.0366 0.0389 0.0504 0.0344 0.0673 0.0433 0.0291 0.0625 0.0643 0.0551 0.0699 0.0328 0.0353 0.0207 0.0703 0.1188
Mitochondria 0.0223 0.0245 0.0205 0.0403 0.0268 0.0223 0.0072 0.0071 0.0129 0.0028 0.0352 0.0091 0.0189 0.0129 0.0225 0.1458 0.0167 0.0186
None 0.092 0.2117 0.1781 0.0757 0.1354 0.1669 0.2095 0.3033 0.2251 0.1574 0.2287 0.2002 0.0908 0.2035 0.1244 0.1144 0.0342 0.1094
Nuclear Periphery 0.0126 0.0013 0.0012 0.0008 0.0009 0.0038 0.0079 0.0005 0.0107 0.0001 0.0117 0.005 0.0272 0.0006 0.0126 0.0001 0.0625 0.002
Nucleolus 0.0057 0.0038 0.001 0.0012 0.008 0.0008 0.0013 0.0018 0.0006 0.0002 0.0008 0.0008 0.0009 0.001 0.0027 0.0004 0.0019 0.0009
Nucleus 0.0135 0.0035 0.0018 0.0015 0.0019 0.0027 0.0031 0.003 0.0027 0.0001 0.0046 0.0021 0.0169 0.0011 0.0104 0.0002 0.0224 0.0018
Peroxisomes 0.3865 0.3388 0.4091 0.4984 0.4583 0.3938 0.2465 0.2387 0.313 0.4736 0.2552 0.3742 0.1961 0.1981 0.347 0.4139 0.2547 0.2958
Punctate Nuclear 0.0169 0.0165 0.0105 0.007 0.0607 0.012 0.02 0.0198 0.0126 0.0024 0.0201 0.0096 0.0121 0.0051 0.0087 0.0007 0.0084 0.0122
Vacuole 0.0032 0.0021 0.0015 0.0029 0.0012 0.0015 0.0017 0.0019 0.0012 0.0002 0.001 0.0013 0.0014 0.0026 0.0029 0.0008 0.0116 0.0029
Vacuole Periphery 0.0017 0.0006 0.0006 0.0007 0.0007 0.0006 0.0007 0.0003 0.0013 0.0001 0.0006 0.0007 0.0019 0.0005 0.001 0.0003 0.0085 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.195 7.8491 2.5886 6.9243 4.4272 9.7506 9.6999 10.4153 9.3201 9.5955
Translational Efficiency 0.2761 0.5183 1.0898 0.563 0.8417 0.4643 0.5207 0.5448 0.4235 0.5772

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
191 781 138 1671 721 2468 198 1189 912 3249 336 2860

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 640.43 671.37 776.06 798.86 636.89 717.40 853.12 801.77 637.63 706.34 821.47 800.07
Standard Deviation 93.41 100.51 95.14 118.28 75.01 90.21 85.01 115.93 79.23 94.85 97.02 117.32
Intensity Change Log 2 — 0.068067 0.277127 0.318902 — 0.171734 0.421704 0.332144 — 0.120688 0.351026 0.325520

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.013814 0.022360 0.009630 0.093178 0.010106 0.014893 0.007539 0.082126 0.010882 0.016688 0.008398 0.088583
Bud Neck 0.032437 0.009818 0.004494 0.017301 0.015274 0.010600 0.012377 0.013242 0.018868 0.010412 0.009139 0.015613
Bud Site 0.018971 0.012222 0.013297 0.075474 0.006009 0.008646 0.014467 0.076976 0.008724 0.009506 0.013986 0.076099
Cell Periphery 0.000993 0.000236 0.000426 0.001048 0.000289 0.000130 0.000615 0.000548 0.000437 0.000155 0.000537 0.000840
Cytoplasm 0.071196 0.026916 0.046650 0.115572* 0.058332 0.027796 0.113572* 0.143293* 0.061026 0.027585 0.086086 0.127096*
Cytoplasmic Foci 0.250296* 0.218108* 0.232161* 0.138539 0.319000* 0.238622* 0.314860* 0.142663 0.304611* 0.233691* 0.280895* 0.140253
Eisosomes 0.002530 0.000548 0.000323 0.001150 0.001390 0.000393 0.000556 0.000713 0.001629 0.000430 0.000460 0.000968
Endoplasmic Reticulum 0.001984 0.000408 0.002106 0.003181 0.000416 0.000356 0.002657 0.001930 0.000744 0.000368 0.002431 0.002661
Endosome 0.012748 0.066914 0.038854 0.021612 0.025081 0.058407 0.025661 0.014696 0.022498 0.060452 0.031079 0.018737
Golgi 0.024436 0.089672 0.005742 0.007127 0.035665 0.089559 0.002610 0.007418 0.033313 0.089586 0.003896 0.007248
Lipid Particles 0.033695 0.032887 0.043851 0.014159 0.042172 0.028065 0.030922 0.010508 0.040397 0.029224 0.036232 0.012641
Mitochondria 0.042952 0.034540 0.001652 0.006223 0.015115 0.043532 0.001125 0.004112 0.020945 0.041371 0.001341 0.005345
Mitotic Spindle 0.008149 0.008834 0.031541 0.027722 0.002344 0.018571 0.004227 0.035961 0.003560 0.016230 0.015445 0.031147
None 0.004725 0.000736 0.000993 0.000398 0.001389 0.000422 0.003172 0.001288 0.002088 0.000498 0.002277 0.000768
Nuclear Periphery 0.000342 0.000086 0.000470 0.000340 0.000185 0.000070 0.000747 0.000443 0.000218 0.000074 0.000633 0.000383
Nuclear Periphery Foci 0.002515 0.000320 0.010718 0.001537 0.000336 0.000218 0.004367 0.001359 0.000793 0.000242 0.006975 0.001463
Nucleolus 0.000744 0.001421 0.003649 0.000515 0.000739 0.000466 0.002455 0.000410 0.000740 0.000696 0.002945 0.000472
Nucleus 0.036789 0.004345 0.076894 0.050657 0.020194 0.004043 0.144425* 0.043287 0.023669 0.004115 0.116689 0.047593
Peroxisomes 0.431056* 0.462432* 0.445988* 0.345361* 0.440291* 0.447349* 0.247321* 0.334441* 0.438357* 0.450974* 0.328916* 0.340822*
Vacuole 0.008320 0.006457 0.030339 0.076517 0.005165 0.006713 0.066117 0.082503 0.005826 0.006652 0.051423 0.079006
Vacuole Periphery 0.001307 0.000740 0.000220 0.002388 0.000508 0.001150 0.000210 0.002080 0.000675 0.001051 0.000214 0.002260

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.44 0.77 -9.66 -11.86 -15.48 -2.86 1.38 -12.03 -11.14 -12.52 -3.19 1.37 -18.64 -17.94 -20.31
Bud Neck 2.77 3.42 1.75 -4.14 -8.74 1.71 1.05 0.50 -2.16 -0.86 3.08 3.24 0.87 -5.05 -3.87
Bud Site 1.37 1.02 -8.54 -13.86 -12.22 -2.12 -2.67 -13.89 -13.43 -8.62 -0.61 -2.42 -19.41 -20.28 -13.79
Cell Periphery 4.31 2.90 0.29 -5.65 -3.56 2.54 -3.65 -3.10 -10.16 1.60 4.48 -1.64 -3.69 -9.17 -2.27
Cytoplasm 4.11 0.60 -4.99 -18.19 -5.02 5.88 -4.41 -12.55 -20.16 -3.31 7.14 -3.24 -13.66 -28.15 -5.06
Cytoplasmic Foci 1.84 0.69 6.71 10.61 5.65 8.58 0.63 17.64 15.20 9.57 8.55 1.92 19.80 20.65 11.09
Eisosomes 2.24 2.45 1.69 -3.71 -6.66 2.66 2.20 1.94 -3.98 -1.22 3.43 3.28 2.12 -6.84 -5.90
Endoplasmic Reticulum 1.65 -1.53 -1.95 -10.02 0.03 0.32 -4.50 -10.16 -8.99 1.38 1.51 -5.17 -9.08 -12.37 0.78
Endosome -10.93 -3.13 -3.00 9.83 2.26 -9.36 0.00 3.91 16.79 3.08 -12.62 -1.81 2.16 18.56 3.28
Golgi -9.38 4.05 3.91 16.35 -0.65 -13.44 11.62 9.88 27.43 -2.78 -16.14 11.98 10.89 32.67 -3.07
Lipid Particles 0.18 -1.56 3.54 4.98 4.59 3.57 1.61 8.00 9.01 4.54 3.35 0.42 8.27 9.47 6.26
Mitochondria 0.98 5.37 4.88 7.91 -4.56 -8.65 6.00 4.82 16.27 -3.08 -6.51 7.91 6.51 17.88 -5.85
Mitotic Spindle -0.16 -2.62 -4.26 -5.37 0.82 -8.97 -1.29 -8.42 -3.68 -5.05 -7.88 -3.23 -11.02 -5.03 -2.20
None 1.08 0.96 1.20 0.91 2.10 1.28 -0.72 0.47 -1.56 1.30 1.65 0.14 1.44 -0.90 1.98
Nuclear Periphery 1.23 -2.12 -1.39 -8.08 1.53 1.84 -3.66 -3.62 -5.02 1.91 2.19 -4.50 -4.89 -9.36 2.51
Nuclear Periphery Foci 0.98 -2.71 0.16 -8.58 3.08 1.66 -4.60 -3.67 -3.93 2.41 1.23 -4.57 -2.89 -7.17 3.87
Nucleolus -1.18 -3.33 -0.32 1.07 3.28 0.96 -2.60 0.94 -0.20 3.61 0.19 -4.00 0.58 0.41 4.40
Nucleus 3.94 -3.21 -2.95 -17.39 1.97 5.62 -8.36 -8.68 -15.33 5.66 6.84 -8.89 -10.25 -23.52 5.62
Peroxisomes -1.20 1.77 5.22 12.03 2.49 -0.76 10.15 11.22 15.90 -2.75 -1.26 8.31 12.30 20.23 -0.60
Vacuole 0.61 -4.73 -21.35 -24.11 -7.39 -1.63 -8.61 -21.72 -21.55 -4.03 -0.92 -9.85 -32.18 -32.40 -6.88
Vacuole Periphery 1.27 2.36 -0.91 -2.46 -3.52 -3.19 2.36 -1.55 -0.52 -2.04 -2.09 3.24 -2.65 -1.69 -3.98
Standard name
Human Ortholog
Description Peripheral peroxisomal membrane protein; involved in controlling peroxisome size and number, interacts with Pex25p; PEX27 has a paralog, PEX25, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (12%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Pex27

Pex27


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pex27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available