Standard name
Description Protein involved in biosynthesis of the coenzyme lipoic acid; has similarity to E. coli lipoic acid synthase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.08 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.08 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.29 0.46 0.42 0.22 0.2 0.1 0 0.09 0 0.1 0.11 0.09
Mitochondria 0.96 0.94 0.94 0.96 0.9 0.94 0.93 0.9 0.93 0.92 0.94 0.94 0.85 0.65 0.71 0.07 0.07 0.09 0.86 0.79 0.83 0.74 0.7 0.71
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0.06 0 0 0 0.11 0.1 0.12 0.13 0.08 0.1 0.11 0.05 0 0 0.06 0.11 0.15 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0.07 0.12 0.09 0.1 0.1 0.08 0.07 0.12 0 0 0 0.09 0.08 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0.09 0 0.06 0 0 0 0 0 0 0 0 0 0 0.12 0.14 0.18 0.05 0 0.07 0 0 0.06
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.07 0 0 0.05 0.05 0 0 0 0 0 0.05 0.11 0.18 0.11 0.18 0.17 0.16 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 5 3 1 1 0 0 0 2 2 0 0 3 2 9 6 12 7 3 4 3 6 10 11
Bud 0 1 6 6 6 6 7 6 8 10 7 7 1 0 3 9 8 4 1 0 0 2 4 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 1 1 0 0 2 2 6 3 3 3 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 8 5 4 3 3 6 4 3 3 1 1 13 16 23 9 13 7 0 0 1 0 1 1
Cytoplasm 4 1 3 6 4 1 0 6 4 2 4 1 10 10 6 5 10 7 0 0 1 0 0 0
Endoplasmic Reticulum 2 3 2 0 0 0 2 1 3 0 2 0 0 5 4 2 6 1 0 0 1 0 1 1
Endosome 1 1 2 0 4 0 1 1 2 0 0 1 11 17 13 9 6 6 4 7 3 9 13 12
Golgi 1 5 7 2 1 0 0 1 0 0 0 0 135 252 228 22 27 9 6 22 7 32 45 37
Mitochondria 233 231 221 132 169 249 281 257 320 305 271 285 398 353 388 7 9 8 204 205 192 240 294 300
Nucleus 0 0 0 0 1 1 1 1 1 2 2 1 3 0 4 2 0 4 0 0 0 0 4 0
Nuclear Periphery 16 14 8 6 3 30 29 34 43 28 28 32 22 20 23 6 14 14 0 1 0 1 4 11
Nucleolus 16 7 10 2 13 32 26 29 36 28 19 36 8 8 7 9 10 10 2 1 0 2 3 2
Peroxisomes 4 3 21 3 12 6 7 6 0 10 1 1 8 11 16 12 18 17 11 11 17 14 10 26
SpindlePole 1 0 1 0 1 1 4 3 6 4 1 1 3 5 8 3 3 1 0 0 0 2 2 2
Vac/Vac Membrane 8 16 4 3 9 12 7 6 4 9 9 16 50 99 60 18 23 15 2 3 1 9 20 10
Unique Cell Count 242 245 234 138 188 266 302 285 343 332 289 304 467 544 543 101 132 93 237 261 232 327 421 424
Labelled Cell Count 289 296 293 165 229 343 377 358 435 406 346 384 665 798 792 119 159 110 237 261 232 327 421 424


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 11.2 9.9 9.3 9.2 8.0 8.1 8.2 7.5 7.9 7.4 7.5 12.0 11.5 10.7 12.7 12.3 12.6 12.2 12.7 13.6
Std Deviation (1e-4) 2.3 2.8 1.8 1.7 2.0 2.0 2.1 2.3 2.1 1.9 2.1 1.9 2.0 1.9 1.7 3.4 2.9 3.5 2.9 2.7 2.7
Intensity Change (Log2) – – – -0.1 -0.11 -0.31 -0.3 -0.28 -0.4 -0.34 -0.42 -0.41 0.27 0.22 0.1 0.35 0.31 0.35 0.3 0.36 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 3.6 0
Bud 0 0.4 -0.2 -0.2 -0.3 -0.2 0.3 -0.1 -0.2 0 0 0 0 1.7 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.5 0.6 1.4 0 3.3 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 1.9 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 8.1 11.8 10.9 5.6 5.5 0
Mitochondria 0.5 -1.8 -0.4 -0.7 -1.8 -0.6 -1.2 -0.3 -0.3 -3.6 -8.6 -7.1 -15.8 -16.7 -15.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.5 0 3.3 2.8 3.5 3.8 2.4 2.8 3.1 0.8 0.2 0.5 0 2.8 3.8
Nucleolus 0 1.2 3.1 2.0 2.5 2.7 1.9 1.1 3.1 -2.0 -2.4 -2.6 1.7 1.3 2.2
Peroxisomes -2.6 -1.0 -3.3 -3.4 -3.5 -5.7 -3.1 -4.9 -5.0 -4.6 -4.5 -3.6 0.8 1.4 2.4
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.8 1.8 0 0 0 0.8 1.0 2.2 4.2 6.2 4.3 5.5 5.5 5.0