Standard name | |
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Description | Protein involved in biosynthesis of the coenzyme lipoic acid; has similarity to E. coli lipoic acid synthase |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 5 | 3 | 1 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 3 | 2 | 9 | 6 | 12 | 7 | 3 | 4 | 3 | 6 | 10 | 11 |
Bud | 0 | 1 | 6 | 6 | 6 | 6 | 7 | 6 | 8 | 10 | 7 | 7 | 1 | 0 | 3 | 9 | 8 | 4 | 1 | 0 | 0 | 2 | 4 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 1 | 1 | 0 | 0 | 2 | 2 | 6 | 3 | 3 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 8 | 5 | 4 | 3 | 3 | 6 | 4 | 3 | 3 | 1 | 1 | 13 | 16 | 23 | 9 | 13 | 7 | 0 | 0 | 1 | 0 | 1 | 1 |
Cytoplasm | 4 | 1 | 3 | 6 | 4 | 1 | 0 | 6 | 4 | 2 | 4 | 1 | 10 | 10 | 6 | 5 | 10 | 7 | 0 | 0 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 2 | 3 | 2 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 2 | 0 | 0 | 5 | 4 | 2 | 6 | 1 | 0 | 0 | 1 | 0 | 1 | 1 |
Endosome | 1 | 1 | 2 | 0 | 4 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 11 | 17 | 13 | 9 | 6 | 6 | 4 | 7 | 3 | 9 | 13 | 12 |
Golgi | 1 | 5 | 7 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 135 | 252 | 228 | 22 | 27 | 9 | 6 | 22 | 7 | 32 | 45 | 37 |
Mitochondria | 233 | 231 | 221 | 132 | 169 | 249 | 281 | 257 | 320 | 305 | 271 | 285 | 398 | 353 | 388 | 7 | 9 | 8 | 204 | 205 | 192 | 240 | 294 | 300 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 3 | 0 | 4 | 2 | 0 | 4 | 0 | 0 | 0 | 0 | 4 | 0 |
Nuclear Periphery | 16 | 14 | 8 | 6 | 3 | 30 | 29 | 34 | 43 | 28 | 28 | 32 | 22 | 20 | 23 | 6 | 14 | 14 | 0 | 1 | 0 | 1 | 4 | 11 |
Nucleolus | 16 | 7 | 10 | 2 | 13 | 32 | 26 | 29 | 36 | 28 | 19 | 36 | 8 | 8 | 7 | 9 | 10 | 10 | 2 | 1 | 0 | 2 | 3 | 2 |
Peroxisomes | 4 | 3 | 21 | 3 | 12 | 6 | 7 | 6 | 0 | 10 | 1 | 1 | 8 | 11 | 16 | 12 | 18 | 17 | 11 | 11 | 17 | 14 | 10 | 26 |
SpindlePole | 1 | 0 | 1 | 0 | 1 | 1 | 4 | 3 | 6 | 4 | 1 | 1 | 3 | 5 | 8 | 3 | 3 | 1 | 0 | 0 | 0 | 2 | 2 | 2 |
Vac/Vac Membrane | 8 | 16 | 4 | 3 | 9 | 12 | 7 | 6 | 4 | 9 | 9 | 16 | 50 | 99 | 60 | 18 | 23 | 15 | 2 | 3 | 1 | 9 | 20 | 10 |
Unique Cell Count | 242 | 245 | 234 | 138 | 188 | 266 | 302 | 285 | 343 | 332 | 289 | 304 | 467 | 544 | 543 | 101 | 132 | 93 | 237 | 261 | 232 | 327 | 421 | 424 |
Labelled Cell Count | 289 | 296 | 293 | 165 | 229 | 343 | 377 | 358 | 435 | 406 | 346 | 384 | 665 | 798 | 792 | 119 | 159 | 110 | 237 | 261 | 232 | 327 | 421 | 424 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.1 | 11.2 | 9.9 | 9.3 | 9.2 | 8.0 | 8.1 | 8.2 | 7.5 | 7.9 | 7.4 | 7.5 | 12.0 | 11.5 | 10.7 | 12.7 | 12.3 | 12.6 | 12.2 | 12.7 | 13.6 |
Std Deviation (1e-4) | 2.3 | 2.8 | 1.8 | 1.7 | 2.0 | 2.0 | 2.1 | 2.3 | 2.1 | 1.9 | 2.1 | 1.9 | 2.0 | 1.9 | 1.7 | 3.4 | 2.9 | 3.5 | 2.9 | 2.7 | 2.7 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.11 | -0.31 | -0.3 | -0.28 | -0.4 | -0.34 | -0.42 | -0.41 | 0.27 | 0.22 | 0.1 | 0.35 | 0.31 | 0.35 | 0.3 | 0.36 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 0 |
Bud | 0 | 0.4 | -0.2 | -0.2 | -0.3 | -0.2 | 0.3 | -0.1 | -0.2 | 0 | 0 | 0 | 0 | 1.7 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 0.6 | 1.4 | 0 | 3.3 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8.1 | 11.8 | 10.9 | 5.6 | 5.5 | 0 |
Mitochondria | 0.5 | -1.8 | -0.4 | -0.7 | -1.8 | -0.6 | -1.2 | -0.3 | -0.3 | -3.6 | -8.6 | -7.1 | -15.8 | -16.7 | -15.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.5 | 0 | 3.3 | 2.8 | 3.5 | 3.8 | 2.4 | 2.8 | 3.1 | 0.8 | 0.2 | 0.5 | 0 | 2.8 | 3.8 |
Nucleolus | 0 | 1.2 | 3.1 | 2.0 | 2.5 | 2.7 | 1.9 | 1.1 | 3.1 | -2.0 | -2.4 | -2.6 | 1.7 | 1.3 | 2.2 |
Peroxisomes | -2.6 | -1.0 | -3.3 | -3.4 | -3.5 | -5.7 | -3.1 | -4.9 | -5.0 | -4.6 | -4.5 | -3.6 | 0.8 | 1.4 | 2.4 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.8 | 1.8 | 0 | 0 | 0 | 0.8 | 1.0 | 2.2 | 4.2 | 6.2 | 4.3 | 5.5 | 5.5 | 5.0 |