Standard name | |
---|---|
Human Ortholog | |
Description | Component of mRNP complexes associated with polyribosomes; involved in localization of mRNAs to P bodies; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 10 | 3 | 1 | 10 | 2 | 9 | 11 | 14 | 3 | 12 | 7 | 8 | 9 | 3 | 1 | 2 | 0 | 1 | 8 | 1 | 3 | 11 | 27 | 16 |
Bud | 3 | 1 | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 1 | 0 | 1 | 4 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 4 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 5 | 0 | 0 | 3 | 0 | 5 | 3 | 3 | 4 | 4 | 7 | 45 | 49 | 34 | 43 | 46 | 37 | 1 | 0 | 0 | 5 | 13 | 3 |
Cytoplasm | 52 | 25 | 2 | 1 | 2 | 3 | 12 | 18 | 2 | 5 | 4 | 7 | 31 | 137 | 224 | 114 | 101 | 107 | 12 | 8 | 0 | 4 | 9 | 20 |
Endoplasmic Reticulum | 121 | 84 | 17 | 18 | 7 | 31 | 71 | 94 | 25 | 55 | 26 | 20 | 143 | 181 | 119 | 8 | 6 | 6 | 165 | 99 | 44 | 140 | 214 | 170 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 9 | 4 | 3 | 17 | 11 | 15 | 24 | 21 | 3 | 6 | 11 | 4 | 9 | 10 | 0 | 53 | 47 | 25 | 8 | 3 | 5 | 8 | 18 | 26 |
Mitochondria | 1 | 0 | 0 | 13 | 4 | 19 | 19 | 20 | 10 | 29 | 13 | 14 | 0 | 0 | 0 | 11 | 2 | 6 | 3 | 0 | 0 | 1 | 5 | 4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 5 |
Unique Cell Count | 174 | 108 | 20 | 34 | 19 | 51 | 110 | 138 | 36 | 76 | 48 | 39 | 206 | 329 | 339 | 197 | 176 | 166 | 202 | 117 | 61 | 177 | 298 | 263 |
Labelled Cell Count | 198 | 122 | 23 | 59 | 31 | 78 | 154 | 174 | 48 | 115 | 65 | 63 | 241 | 380 | 379 | 233 | 202 | 184 | 202 | 117 | 61 | 177 | 298 | 263 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 45.5 | 58.5 | 41.7 | 38.6 | 43.8 | 39.9 | 31.1 | 29.1 | 30.2 | 28.2 | 26.1 | 26.2 | 47.1 | 52.0 | 48.9 | 65.0 | 71.6 | 70.5 | 40.7 | 47.1 | 53.4 |
Std Deviation (1e-4) | 7.7 | 8.5 | 12.1 | 11.0 | 10.3 | 9.2 | 8.6 | 10.0 | 6.0 | 6.2 | 5.5 | 8.0 | 11.8 | 9.0 | 8.8 | 21.6 | 23.3 | 21.6 | 11.7 | 13.3 | 17.7 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.07 | -0.06 | -0.42 | -0.52 | -0.47 | -0.57 | -0.67 | -0.67 | 0.18 | 0.32 | 0.23 | 0.64 | 0.78 | 0.76 | -0.04 | 0.18 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 5.0 | 4.1 | 4.0 | 4.7 |
Endoplasmic Reticulum | -2.4 | -3.1 | -2.0 | -1.8 | -1.5 | -1.3 | -1.2 | -2.4 | -2.5 | -1.5 | -2.6 | -4.5 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 2.6 | 2.8 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.2 | 1.1 | 0 |
Mitochondria | 0 | 0 | 3.2 | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 35.7379 | 48.1602 | 45.0492 | 44.9957 | 34.7788 | 43.5 | 37.8582 | 45.3261 | 43.0919 | 37.1088 | 35.8206 | 42.088 | 44.5134 | 46.3075 | 46.2898 | 43.7259 | 37.3335 | 44.2755 |
Actin | 0.03 | 0.0195 | 0.0049 | 0.0005 | 0.0069 | 0.0005 | 0.044 | 0.0137 | 0.044 | 0.0068 | 0.0089 | 0.0051 | 0.0135 | 0.0256 | 0.0147 | 0.0141 | 0.0352 | 0.0114 |
Bud | 0.001 | 0.0011 | 0.0003 | 0 | 0.0005 | 0 | 0.0008 | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0004 | 0.0049 | 0.0005 |
Bud Neck | 0.0066 | 0.0009 | 0.0008 | 0.0004 | 0.0022 | 0.0004 | 0.0008 | 0.0009 | 0.002 | 0.0012 | 0.0008 | 0.0017 | 0.0006 | 0.0012 | 0.0005 | 0.004 | 0.0045 | 0.0019 |
Bud Periphery | 0.0019 | 0.0034 | 0.0007 | 0.0003 | 0.0012 | 0.0001 | 0.0013 | 0.0011 | 0.001 | 0.0013 | 0.001 | 0.0003 | 0.0009 | 0.0005 | 0.0003 | 0.0018 | 0.014 | 0.0016 |
Bud Site | 0.0111 | 0.0069 | 0.0003 | 0 | 0.0005 | 0 | 0.0028 | 0.0018 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0.0017 | 0.0002 | 0.0005 | 0.001 | 0.0003 |
Cell Periphery | 0.0061 | 0.0194 | 0.0167 | 0.0086 | 0.0071 | 0.0022 | 0.0041 | 0.0175 | 0.0104 | 0.0164 | 0.0011 | 0.0033 | 0.0123 | 0.0168 | 0.007 | 0.0156 | 0.0126 | 0.0026 |
Cytoplasm | 0.0385 | 0.0784 | 0.0434 | 0.0077 | 0.0118 | 0.022 | 0.0589 | 0.043 | 0.009 | 0.0104 | 0.0153 | 0.0174 | 0.0251 | 0.0521 | 0.028 | 0.05 | 0.0351 | 0.0248 |
Cytoplasmic Foci | 0.031 | 0.0114 | 0.0102 | 0.0002 | 0.0603 | 0.0087 | 0.0077 | 0.0056 | 0.0042 | 0.0026 | 0.0031 | 0.0019 | 0.0084 | 0.0068 | 0.0041 | 0.0025 | 0.0058 | 0.0023 |
Eisosomes | 0.0014 | 0.0022 | 0.0043 | 0.0006 | 0.0003 | 0.0004 | 0.0012 | 0.0032 | 0.0145 | 0.0143 | 0.0002 | 0.0011 | 0.0021 | 0.0079 | 0.0012 | 0.0059 | 0.0021 | 0.0011 |
Endoplasmic Reticulum | 0.5374 | 0.677 | 0.7664 | 0.8685 | 0.6185 | 0.8169 | 0.6515 | 0.7383 | 0.6819 | 0.7877 | 0.6632 | 0.7563 | 0.6885 | 0.7011 | 0.8035 | 0.7747 | 0.5881 | 0.7583 |
Endosome | 0.0408 | 0.0141 | 0.012 | 0.0075 | 0.0558 | 0.012 | 0.0302 | 0.0166 | 0.0185 | 0.0194 | 0.0341 | 0.024 | 0.019 | 0.0165 | 0.0132 | 0.0175 | 0.0267 | 0.0195 |
Golgi | 0.0208 | 0.0617 | 0.0564 | 0.0623 | 0.0852 | 0.0634 | 0.0345 | 0.0943 | 0.1455 | 0.0591 | 0.1473 | 0.0903 | 0.0328 | 0.0983 | 0.0737 | 0.0752 | 0.135 | 0.0948 |
Lipid Particles | 0.025 | 0.0304 | 0.0218 | 0.0003 | 0.0566 | 0.0051 | 0.0107 | 0.0162 | 0.0174 | 0.0044 | 0.0017 | 0.0075 | 0.0121 | 0.032 | 0.02 | 0.0053 | 0.0129 | 0.0048 |
Mitochondria | 0.0335 | 0.0107 | 0.0187 | 0.0018 | 0.025 | 0.0044 | 0.0126 | 0.0124 | 0.005 | 0.032 | 0.0564 | 0.0086 | 0.0063 | 0.006 | 0.0094 | 0.0046 | 0.04 | 0.0066 |
None | 0.0049 | 0.0005 | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0.0129 | 0.0008 | 0.0022 | 0.0006 | 0.0005 | 0.0003 | 0.0021 | 0.0003 | 0.0003 | 0.0004 | 0.0046 | 0.0006 |
Nuclear Periphery | 0.1336 | 0.0382 | 0.0274 | 0.0324 | 0.015 | 0.0553 | 0.0886 | 0.0209 | 0.0302 | 0.0264 | 0.0537 | 0.0638 | 0.1418 | 0.0243 | 0.0159 | 0.0184 | 0.0292 | 0.0574 |
Nucleolus | 0.0011 | 0.0003 | 0.0002 | 0 | 0.0003 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0012 | 0.0017 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0003 |
Nucleus | 0.0118 | 0.0016 | 0.001 | 0.0009 | 0.0006 | 0.0007 | 0.006 | 0.0009 | 0.0028 | 0.0007 | 0.0018 | 0.0073 | 0.0057 | 0.0007 | 0.0006 | 0.0011 | 0.0022 | 0.0023 |
Peroxisomes | 0.0162 | 0.0006 | 0.0008 | 0 | 0.0048 | 0.0002 | 0.0008 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0 | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0001 |
Punctate Nuclear | 0.0105 | 0.0003 | 0.0004 | 0 | 0.003 | 0.0001 | 0.0063 | 0.0002 | 0.001 | 0.0001 | 0.0001 | 0.0005 | 0.0054 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0004 |
Vacuole | 0.0174 | 0.0143 | 0.0065 | 0.0039 | 0.0196 | 0.0031 | 0.0085 | 0.0078 | 0.0042 | 0.006 | 0.0035 | 0.003 | 0.0095 | 0.0044 | 0.0038 | 0.004 | 0.017 | 0.0032 |
Vacuole Periphery | 0.0191 | 0.007 | 0.0063 | 0.0038 | 0.0244 | 0.0045 | 0.0151 | 0.0043 | 0.0052 | 0.0096 | 0.0069 | 0.0064 | 0.0104 | 0.0029 | 0.0029 | 0.0036 | 0.0267 | 0.0051 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 274.4845 | 236.6465 | 186.9979 | 217.3098 | 227.8045 | 314.5264 | 221.0982 | 193.6489 | 205.2746 | 234.0687 |
Translational Efficiency | 3.0816 | 3.1277 | 3.1154 | 2.484 | 3.107 | 2.4995 | 2.2072 | 2.5351 | 2.352 | 2.3611 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1043 | 735 | 175 | 16 | 1133 | 28 | 100 | 20 | 2176 | 763 | 275 | 36 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2054.37 | 2227.59 | 3426.06 | 332.77 | 2575.52 | 2464.41 | 5053.71 | 2842.21 | 2325.72 | 2236.28 | 4017.93 | 1726.90 |
Standard Deviation | 534.27 | 594.76 | 1013.92 | 3.41 | 529.00 | 611.63 | 673.74 | 652.48 | 591.87 | 597.05 | 1196.79 | 1338.44 |
Intensity Change Log 2 | — | 0.116788 | 0.737854 | -2.626099 | — | -0.063621 | 0.972479 | 0.142149 | — | 0.019225 | 0.873047 | -0.544230 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.011557 | 0.014035 | 0.007920 | 0.000133 | 0.006498 | 0.001829 | 0.000890 | 0.006630 | 0.008923 | 0.013587 | 0.005364 | 0.003743 |
Bud Neck | 0.001830 | 0.001526 | 0.004742 | 0.000045 | 0.000922 | 0.002723 | 0.016650 | 0.004302 | 0.001357 | 0.001570 | 0.009072 | 0.002410 |
Bud Site | 0.022551 | 0.005527 | 0.022905 | 0.000068 | 0.001976 | 0.010687 | 0.001644 | 0.005554 | 0.011838 | 0.005716 | 0.015174 | 0.003116 |
Cell Periphery | 0.013869 | 0.012251 | 0.017192 | 0.002569 | 0.005987 | 0.005864 | 0.022233 | 0.050146 | 0.009765 | 0.012016 | 0.019025 | 0.029001 |
Cytoplasm | 0.016388 | 0.001381 | 0.003594 | 0.000902 | 0.000531 | 0.007055 | 0.000297 | 0.000090 | 0.008132 | 0.001589 | 0.002395 | 0.000451 |
Cytoplasmic Foci | 0.069344 | 0.004972 | 0.013941 | 0.000032 | 0.001047 | 0.013475 | 0.000487 | 0.001748 | 0.033783 | 0.005284 | 0.009049 | 0.000985 |
Eisosomes | 0.001698 | 0.005334 | 0.000695 | 0.005121 | 0.003759 | 0.000271 | 0.003282 | 0.002405 | 0.002771 | 0.005148 | 0.001636 | 0.003612 |
Endoplasmic Reticulum | 0.623983* | 0.565660* | 0.238843* | 0.000007 | 0.879172* | 0.650950* | 0.455326* | 0.520251* | 0.756855* | 0.568789* | 0.317564* | 0.289031* |
Endosome | 0.030490 | 0.017877 | 0.037691 | 0.000002 | 0.006366 | 0.046281 | 0.016491 | 0.032555 | 0.017929 | 0.018920 | 0.029982 | 0.018087 |
Golgi | 0.128334* | 0.230952* | 0.259072* | 0.000020 | 0.059146 | 0.094473 | 0.062095 | 0.183350* | 0.092309 | 0.225944* | 0.187444* | 0.101870 |
Lipid Particles | 0.040981 | 0.083496 | 0.103006* | 0.000319 | 0.014602 | 0.109228* | 0.129948* | 0.151961* | 0.027246 | 0.084440 | 0.112803* | 0.084565 |
Mitochondria | 0.002836 | 0.006239 | 0.003895 | 0.000065 | 0.002345 | 0.003280 | 0.000890 | 0.000065 | 0.002580 | 0.006131 | 0.002802 | 0.000065 |
Mitotic Spindle | 0.002271 | 0.000203 | 0.004265 | 0.000477 | 0.000169 | 0.001131 | 0.000525 | 0.000309 | 0.001176 | 0.000237 | 0.002905 | 0.000384 |
None | 0.000583 | 0.002167 | 0.000623 | 0.987003* | 0.000729 | 0.000341 | 0.000134 | 0.000101 | 0.000659 | 0.002100 | 0.000445 | 0.438724* |
Nuclear Periphery | 0.000770 | 0.001357 | 0.004822 | 0.000002 | 0.001997 | 0.000964 | 0.057960 | 0.001134 | 0.001408 | 0.001343 | 0.024145 | 0.000631 |
Nuclear Periphery Foci | 0.006306 | 0.002328 | 0.022761 | 0.000004 | 0.001893 | 0.004679 | 0.059996 | 0.002801 | 0.004008 | 0.002414 | 0.036301 | 0.001558 |
Nucleolus | 0.000691 | 0.001949 | 0.000071 | 0.000007 | 0.000024 | 0.000028 | 0.000073 | 0.000004 | 0.000343 | 0.001878 | 0.000072 | 0.000005 |
Nucleus | 0.000759 | 0.004606 | 0.000322 | 0.000038 | 0.000279 | 0.004896 | 0.000307 | 0.000080 | 0.000509 | 0.004616 | 0.000317 | 0.000061 |
Peroxisomes | 0.000507 | 0.000285 | 0.003407 | 0.003185 | 0.000074 | 0.000089 | 0.000006 | 0.000086 | 0.000281 | 0.000278 | 0.002170 | 0.001464 |
Vacuole | 0.010882 | 0.014363 | 0.172659 | 0.000002 | 0.004190 | 0.032221 | 0.137978 | 0.029665 | 0.007398 | 0.015018 | 0.160048 | 0.016481 |
Vacuole Periphery | 0.013371 | 0.023493 | 0.077573 | 0.000000 | 0.008295 | 0.009536 | 0.032786 | 0.006762 | 0.010728 | 0.022981 | 0.061287 | 0.003757 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.77 | 0.73 | 6.14 | 5.30 | 1.68 | 2.30 | 3.62 | -0.02 | -0.94 | -1.25 | -1.66 | 1.13 | 1.83 | 2.73 | 0.41 |
Bud Neck | 0.77 | -2.21 | 5.06 | 6.04 | 3.13 | -1.31 | -6.16 | -1.31 | -0.37 | 3.47 | -0.72 | -5.39 | -0.70 | -0.54 | 3.61 |
Bud Site | 6.80 | -0.33 | 11.20 | 3.68 | 3.67 | -1.47 | 0.50 | -1.78 | 0.92 | -2.02 | 3.39 | -1.02 | 5.71 | 1.46 | 2.96 |
Cell Periphery | 0.68 | -1.02 | 4.72 | 3.65 | 4.40 | -0.05 | -5.47 | -1.84 | -1.84 | -1.15 | -1.10 | -4.17 | -1.39 | -1.22 | -0.71 |
Cytoplasm | 6.21 | 5.17 | 6.45 | 0.50 | 2.41 | -1.62 | 1.08 | 1.61 | 1.81 | 1.88 | 4.92 | 4.58 | 6.64 | 1.47 | 3.02 |
Cytoplasmic Foci | 14.04 | 10.31 | 15.39 | 6.06 | 5.02 | -1.60 | 2.38 | -1.22 | 1.50 | -2.27 | 11.69 | 8.30 | 14.23 | 4.80 | 4.41 |
Eisosomes | -2.77 | 1.55 | -0.78 | 0.05 | -1.02 | 4.87 | 0.30 | 1.00 | -1.85 | 0.37 | -1.94 | 1.45 | -0.41 | 0.66 | -0.96 |
Endoplasmic Reticulum | 3.66 | 14.62 | 57.07* | 49.88* | 9.67 | 3.66 | 12.33 | 4.44 | 1.19 | -0.83 | 14.49 | 20.37 | 7.47 | 4.42 | 0.35 |
Endosome | 7.09 | -1.57 | 22.00 | 15.95 | 8.68 | -4.39 | -6.75 | -2.49 | 1.01 | -1.49 | -0.72 | -4.07 | -0.02 | 0.13 | 1.72 |
Golgi | -10.22 | -5.80 | 21.99 | 28.23 | 12.15 | -1.65 | -0.24 | -2.27 | -1.51 | -2.21 | -15.48 | -6.08 | -0.29 | 3.56 | 2.22 |
Lipid Particles | -7.27 | -4.59 | 13.64 | 16.53 | 7.82 | -3.01 | -5.79 | -2.80 | -0.77 | -0.41 | -10.95 | -7.65 | -1.92 | -0.01 | 0.88 |
Mitochondria | -1.87 | -0.58 | 4.65 | 3.60 | 2.35 | -0.52 | 1.70 | 2.76 | 1.03 | 3.31 | -2.06 | -0.15 | 4.87 | 3.69 | 2.62 |
Mitotic Spindle | 3.70 | -1.51 | 3.00 | -0.92 | 2.84 | -0.95 | -1.69 | -1.01 | 0.80 | 0.98 | 3.30 | -2.03 | 2.71 | -0.77 | 2.95 |
None | -2.53 | -0.16 | -93.52* | -93.21* | -93.52* | 0.94 | 2.62 | 2.75 | 1.14 | 0.78 | -2.30 | 1.68 | -5.28 | -5.26 | -5.28 |
Nuclear Periphery | -3.73 | -4.61 | 9.06 | 10.29 | 5.49 | 1.19 | -6.94 | 1.79 | 0.06 | 7.05 | 0.29 | -6.78 | 2.78 | 2.81 | 7.00 |
Nuclear Periphery Foci | 4.41 | -4.26 | 7.68 | 6.13 | 5.92 | -2.07 | -5.37 | -1.33 | 1.41 | 5.29 | 2.72 | -6.82 | 4.26 | 1.70 | 7.33 |
Nucleolus | -1.15 | 0.98 | 1.10 | 2.15 | 5.08 | -0.43 | -2.66 | 2.76 | 1.08 | 4.10 | -1.67 | 0.89 | 1.14 | 2.16 | 6.60 |
Nucleus | -2.02 | 1.07 | 2.15 | 2.47 | 2.96 | -0.93 | 0.62 | 1.14 | 1.00 | 4.55 | -2.20 | 1.08 | 2.17 | 2.58 | 3.89 |
Peroxisomes | 1.52 | -0.92 | -0.94 | -1.01 | 0.04 | -0.27 | 2.00 | -0.25 | 0.03 | -2.22 | 0.03 | -0.94 | -0.93 | -0.93 | 0.29 |
Vacuole | -1.45 | -9.17 | 7.97 | 7.43 | 9.81 | -2.38 | -8.97 | -2.95 | 0.31 | 6.38 | -3.74 | -12.24 | -1.67 | -0.23 | 10.66 |
Vacuole Periphery | -4.06 | -7.06 | 10.16 | 11.13 | 8.64 | -0.22 | -3.93 | 0.68 | 0.46 | 3.99 | -5.58 | -8.00 | 4.62 | 7.94 | 9.00 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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