Standard name
Human Ortholog
Description Ribose methyltransferase; modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0.06 0.07 0.05 0 0 0.05 0 0 0 0.07 0.05 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0 0 0 0 0 0 0 0 0.18 0.25 0.43 0.11 0.09 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0.18 0 0.15 0 0 0 0 0 0 0 0.25 0.32 0.22 0.22 0.26 0.22 0.15 0.18 0.11 0.11 0.13 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.06
Mitochondria 0.92 0.86 0.67 0.88 0.77 0.93 0.93 0.92 0.94 0.97 0.97 0.96 0.2 0.06 0.06 0.17 0.15 0.16 0.63 0.57 0.62 0.66 0.6 0.56
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.05 0.36 0.15 0.14 0 0 0 0 0 0 0 0.08 0.28 0.18 0.14 0.22 0.1 0.11 0.06 0.14 0.06 0.07 0.1
SpindlePole 0 0 0.18 0.05 0.08 0.05 0 0.06 0.06 0 0 0 0.29 0.29 0.2 0.3 0.28 0.18 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.08 0 0 0.09 0 0 0 0 0 0.05 0.05 0.1 0.14 0.07 0.09 0.13 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 2 0 0 0 0 0 0 0 0 0 15 1 3 3 3 1 3 7 3 3 10 9
Bud 7 9 21 15 10 14 24 21 20 16 26 25 9 5 9 9 10 6 0 0 1 2 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 3 0 2 1 2 1 0 1 0 0 2 4
Bud Site 0 0 1 2 0 5 10 14 8 10 14 6 0 0 1 2 3 3 – – – – – –
Cell Periphery 2 3 11 4 5 1 3 2 8 5 6 4 7 5 2 7 6 5 0 0 0 0 0 0
Cytoplasm 7 6 14 9 11 11 16 10 14 9 5 11 52 83 142 18 16 18 0 0 0 4 4 5
Endoplasmic Reticulum 2 2 1 1 1 1 2 1 1 5 0 11 9 0 6 5 12 5 0 1 0 1 0 3
Endosome 4 6 56 9 38 2 2 8 6 2 2 2 70 106 72 35 49 27 34 31 35 37 52 46
Golgi 0 0 2 1 0 0 0 0 0 0 0 0 3 1 2 5 6 5 10 8 11 10 16 25
Mitochondria 208 134 205 258 202 355 485 468 454 506 385 498 56 21 21 27 27 20 146 98 197 230 233 229
Nucleus 0 1 0 0 2 4 12 3 5 4 3 1 3 0 1 7 1 4 0 0 0 2 0 2
Nuclear Periphery 3 0 0 1 0 16 14 14 9 14 5 30 0 0 0 0 1 0 0 0 1 1 1 3
Nucleolus 1 1 0 0 5 13 18 16 7 9 10 9 0 0 0 1 1 3 0 0 2 1 1 4
Peroxisomes 4 8 108 44 37 16 8 12 12 1 6 8 22 92 60 22 40 12 26 9 45 21 25 40
SpindlePole 9 7 56 14 20 18 21 30 30 9 10 13 81 95 64 48 52 22 2 3 9 10 14 10
Vac/Vac Membrane 22 13 6 3 23 17 18 20 19 22 21 24 28 48 22 14 24 18 2 6 1 14 15 11
Unique Cell Count 226 156 304 294 262 383 519 509 484 520 396 517 283 332 327 162 186 122 231 171 317 350 387 410
Labelled Cell Count 270 192 483 361 354 473 633 619 593 612 493 643 358 457 407 204 253 150 231 171 317 350 387 410


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.4 4.6 4.6 4.9 3.9 3.7 3.9 3.8 3.6 3.5 3.6 5.9 5.9 5.5 6.3 5.8 5.6 6.3 6.4 7.3
Std Deviation (1e-4) 0.6 0.6 1.2 1.3 1.8 1.3 1.3 1.1 0.9 0.9 0.8 0.8 1.1 1.5 0.9 1.6 1.3 1.5 1.2 1.2 2.0
Intensity Change (Log2) – – – 0.0 0.11 -0.23 -0.28 -0.24 -0.27 -0.34 -0.36 -0.35 0.38 0.37 0.27 0.46 0.36 0.29 0.47 0.48 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.4 4.9 5.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.4609 6.011 6.0397 5.3424 5.4237 6.0483 5.1444 5.6968 5.3933 5.7684 4.9625 5.2961 4.6905 5.1346 4.7286 4.9797 4.4128 4.7509
Actin 0.001 0.0007 0.0013 0.0004 0.0002 0.0004 0.0043 0.0029 0.0011 0.0005 0.0025 0.003 0.0049 0.002 0.0059 0.0001 0.0034 0.0021
Bud 0 0 0.0001 0.0001 0 0 0.0001 0.0003 0.0002 0 0.0003 0.0001 0.0033 0.0002 0.0001 0 0.0001 0
Bud Neck 0 0 0 0 0 0 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0
Bud Periphery 0.0001 0.0001 0.0002 0.0002 0.0001 0 0.0002 0.0005 0.0004 0.0001 0.0006 0.0002 0.0011 0.0005 0.0002 0.0001 0.0001 0.0001
Bud Site 0.0001 0 0.0002 0 0 0 0.0003 0.0009 0.0001 0.0001 0.0004 0.0001 0.0011 0.0008 0.0003 0 0.0001 0
Cell Periphery 0.0001 0 0 0 0 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0002 0.0001 0 0 0
Cytoplasm 0.0001 0 0 0 0 0 0.0011 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0
Cytoplasmic Foci 0.0026 0.0016 0.0009 0.0003 0.0004 0.0008 0.0038 0.0043 0.0047 0.0011 0.002 0.0021 0.0035 0.0035 0.002 0.0001 0.0091 0.0035
Eisosomes 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0008 0.0001 0.001 0.0001 0.0001 0.0005 0.0002 0.0002 0.0002 0 0.0001 0.0001
Endoplasmic Reticulum 0 0 0.0001 0 0 0 0.0003 0.0001 0.0001 0 0 0.0004 0.0001 0.0002 0.0001 0 0.0001 0
Endosome 0.0072 0.0028 0.0027 0.001 0.0017 0.0022 0.0177 0.0139 0.0173 0.0033 0.0031 0.0094 0.0197 0.0149 0.016 0.0004 0.0133 0.0031
Golgi 0.0222 0.0131 0.0061 0.0027 0.0051 0.0158 0.0261 0.0382 0.0216 0.0067 0.0086 0.0272 0.0216 0.0337 0.0284 0.0007 0.0449 0.0179
Lipid Particles 0.005 0.0071 0.0038 0.0007 0.0006 0.0029 0.0201 0.0017 0.002 0.0006 0.0028 0.0045 0.0138 0.0096 0.0017 0.0002 0.011 0.0067
Mitochondria 0.9403 0.9364 0.9735 0.9904 0.9893 0.9693 0.9049 0.9238 0.9412 0.9842 0.9727 0.9433 0.9019 0.902 0.9298 0.9948 0.8953 0.959
None 0 0 0 0 0 0 0.0001 0 0 0 0.0002 0.0002 0.0002 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.0001 0.0001 0 0 0 0.0007 0.0001 0.0004 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.0002 0.0002 0 0 0.0015 0 0.0005 0.0002 0 0 0 0
Nucleus 0 0 0 0 0 0 0.0001 0.0001 0 0 0.0004 0.0001 0.0002 0.0001 0 0 0 0
Peroxisomes 0.0199 0.0374 0.0094 0.0036 0.002 0.0079 0.0155 0.0077 0.0076 0.0027 0.0024 0.0056 0.0221 0.0123 0.0096 0.0031 0.021 0.0061
Punctate Nuclear 0 0 0 0 0 0 0 0 0 0 0 0.0001 0.0001 0 0 0 0 0
Vacuole 0.0004 0.0001 0.0004 0.0001 0.0001 0.0001 0.0017 0.0015 0.0008 0.0002 0.0009 0.0005 0.0021 0.0085 0.0026 0.0001 0.0003 0.0002
Vacuole Periphery 0.0008 0.0005 0.001 0.0003 0.0003 0.0003 0.0025 0.0032 0.0014 0.0003 0.0014 0.0019 0.0031 0.0107 0.0028 0.0003 0.001 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.0527 28.6598 18.3131 21.0824 27.7116 18.6914 29.5816 21.7313 30.0604 28.6921
Translational Efficiency 0.8811 0.6563 0.8939 0.787 0.6762 1.3527 0.9328 1.0523 0.7814 0.9088

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
180 220 1635 1025 1811 1849 219 1300 1991 2069 1854 2325

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 764.21 739.31 927.91 887.84 737.19 769.18 997.62 895.21 739.63 766.00 936.14 891.96
Standard Deviation 92.34 108.69 112.37 109.59 90.93 95.03 111.07 124.36 91.39 97.01 114.45 118.13
Intensity Change Log 2 — -0.047790 0.280016 0.216331 — 0.061285 0.436454 0.280190 — 0.006797 0.358947 0.248039

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000788 0.001645 0.008846 0.035858 0.000402 0.003071 0.009135 0.048030 0.000437 0.002919 0.008881 0.042664
Bud Neck 0.004583 0.019541 0.004741 0.011362 0.005629 0.003154 0.011590 0.011378 0.005535 0.004897 0.005550 0.011371
Bud Site 0.009330 0.029296 0.024755 0.120251* 0.009132 0.006838 0.044910 0.147833* 0.009150 0.009226 0.027135 0.135673*
Cell Periphery 0.000639 0.001060 0.000263 0.000448 0.000300 0.000405 0.000388 0.000903 0.000331 0.000475 0.000278 0.000702
Cytoplasm 0.002732 0.004383 0.005859 0.030857 0.004836 0.003301 0.011964 0.032321 0.004646 0.003416 0.006580 0.031676
Cytoplasmic Foci 0.030757 0.057983 0.095010 0.109376 0.051040 0.066542 0.124313 0.073968 0.049206 0.065632 0.098471 0.089578
Eisosomes 0.000085 0.000109 0.000111 0.000202 0.000067 0.000122 0.000165 0.000259 0.000068 0.000121 0.000118 0.000234
Endoplasmic Reticulum 0.000509 0.000258 0.002488 0.002742 0.000621 0.000217 0.002808 0.001890 0.000611 0.000221 0.002525 0.002266
Endosome 0.109514 0.149732 0.116995 0.083209 0.084812 0.099530 0.110935 0.054869 0.087045 0.104868 0.116279 0.067363
Golgi 0.089234 0.106838 0.133128* 0.088742 0.136292* 0.196782* 0.093422 0.092492 0.132037* 0.187218* 0.128438* 0.090839
Lipid Particles 0.007320 0.002087 0.003885 0.002554 0.007052 0.004653 0.007301 0.000781 0.007077 0.004380 0.004288 0.001562
Mitochondria 0.636273* 0.465624* 0.352158* 0.178266* 0.615999* 0.542647* 0.159724* 0.281818* 0.617832* 0.534457* 0.329427* 0.236166*
Mitotic Spindle 0.013134 0.017468 0.107416* 0.105171* 0.011249 0.009039 0.075596 0.047956 0.011419 0.009935 0.103657* 0.073180
None 0.000209 0.000272 0.000283 0.000178 0.000723 0.000163 0.000202 0.000171 0.000677 0.000175 0.000273 0.000174
Nuclear Periphery 0.000269 0.000053 0.001284 0.000244 0.000232 0.000059 0.000366 0.000073 0.000235 0.000058 0.001175 0.000148
Nuclear Periphery Foci 0.000734 0.000168 0.002052 0.000736 0.000427 0.000202 0.001647 0.000231 0.000455 0.000199 0.002004 0.000454
Nucleolus 0.002432 0.002301 0.001664 0.000329 0.001520 0.000241 0.001461 0.000134 0.001602 0.000460 0.001640 0.000220
Nucleus 0.002187 0.002109 0.003726 0.004342 0.001566 0.000459 0.015626 0.003177 0.001622 0.000634 0.005132 0.003691
Peroxisomes 0.046832 0.121346* 0.056142 0.159072* 0.028134 0.045698 0.267545* 0.168739* 0.029825 0.053742 0.081113 0.164477*
Vacuole 0.016483 0.009547 0.052926 0.056879 0.017841 0.007974 0.050318 0.027289 0.017718 0.008141 0.052618 0.040334
Vacuole Periphery 0.025955 0.008180 0.026270 0.009181 0.022127 0.008901 0.010584 0.005688 0.022473 0.008825 0.024417 0.007228

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.37 -7.57 -10.31 -10.03 -7.53 -3.63 -6.06 -13.89 -12.81 -10.40 -3.78 -9.01 -17.34 -15.73 -12.87
Bud Neck -2.52 -0.27 -4.82 1.31 -6.99 2.75 -2.80 -5.00 -8.15 0.06 0.61 -0.25 -6.49 -6.89 -7.46
Bud Site -2.71 -3.45 -15.54 -11.14 -16.24 1.77 -6.15 -24.38 -24.95 -12.37 -0.06 -10.06 -30.65 -30.74 -25.10
Cell Periphery -1.00 1.74 0.92 1.70 -3.37 -1.42 -1.19 -5.31 -3.92 -4.11 -1.81 1.06 -4.99 -2.33 -5.98
Cytoplasm -1.31 -2.80 -11.38 -11.15 -10.61 1.73 -2.72 -13.90 -15.61 -6.09 1.50 -2.32 -17.00 -19.28 -15.97
Cytoplasmic Foci -4.20 -13.33 -14.27 -7.91 -2.96 -5.41 -9.76 -6.82 -2.22 6.47 -6.17 -15.69 -13.75 -7.88 2.62
Eisosomes -1.66 -2.19 -7.22 -6.72 -7.52 -7.06 -7.45 -18.53 -11.09 -5.16 -7.31 -10.64 -20.54 -11.36 -13.67
Endoplasmic Reticulum 1.25 -6.66 -5.61 -6.63 -1.20 3.44 -4.81 -3.55 -4.73 1.60 3.60 -8.16 -6.30 -8.00 0.25
Endosome -2.25 -0.54 2.18 5.11 6.91 -3.20 -2.67 7.51 10.75 6.11 -3.95 -6.41 5.51 9.75 12.67
Golgi -1.26 -3.84 0.40 2.17 8.14 -8.94 4.62 7.75 16.65 -0.00 -8.79 0.88 8.72 18.12 8.35
Lipid Particles 1.18 0.75 0.99 -1.19 1.46 2.05 -0.31 6.48 5.56 2.95 2.38 2.41 5.44 4.05 4.51
Mitochondria 4.80 10.55 17.12 11.40 15.49* 6.40 23.85 28.07* 21.81 -6.12 7.64 26.94 39.18* 30.45 9.76
Mitotic Spindle -0.77 -14.83 -12.19 -11.98 0.25 1.67 -6.84 -10.76 -11.46 2.68 1.13 -20.61 -17.61 -18.10 5.46
None -1.15 -1.70 0.56 1.74 2.57 4.43 3.95 4.41 -0.34 1.18 4.36 3.38 4.38 -0.06 3.22
Nuclear Periphery 1.44 -3.36 -0.15 -5.04 3.70 3.13 -1.53 2.70 -1.10 3.42 3.40 -3.94 1.11 -4.73 4.26
Nuclear Periphery Foci 1.14 -2.16 -0.43 -4.37 2.59 2.23 -2.54 1.69 -0.72 2.84 2.52 -4.25 -0.97 -3.75 3.97
Nucleolus -0.00 0.32 0.83 0.99 1.65 2.65 -0.04 2.85 1.25 3.99 2.26 -0.02 2.50 0.36 2.66
Nucleus -0.01 -1.13 -2.47 -2.80 -1.88 2.28 -2.53 -1.93 -3.24 2.17 2.07 -3.51 -3.81 -5.55 0.42
Peroxisomes -4.35 -0.90 -8.60 -2.27 -12.58 -5.28 -11.56 -19.96 -16.97 4.57 -6.85 -11.37 -24.89 -19.23 -12.78
Vacuole 1.69 -8.50 -9.27 -13.18 -2.71 6.94 -5.14 -5.22 -9.20 2.94 7.13 -13.85 -11.56 -16.47 1.89
Vacuole Periphery 3.24 -0.03 3.16 -0.43 10.12 9.45 5.30 11.33 3.86 2.45 9.99 -0.98 11.12 2.41 12.23
Standard name
Human Ortholog
Description Ribose methyltransferase; modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrm1

Mrm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available