Standard name
Human Ortholog
Description Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.09 0.09 0.1 0.16 0.07 0.07 0.06 0.07 0.08 0.13 0.21 0.23 0.16 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.52 0 0.1 0 0.2 0.24 0.2 0.67 0.48 0.61 0.58 0 0 0 0.12 0.11 0.12 0 0 0 0 0 0
Nucleus 0.95 0.8 0.97 0.93 0.86 0.79 0.78 0.68 0.62 0.68 0.69 0.62 0.88 0.85 0.75 0.67 0.78 0.85 0.87 0.78 0.82 0.78 0.67 0.55
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0.07 0.06 0.11 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0 0.07 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.06 0.1 0.05 0.06 0.09 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 1 4 3 6 3 0 0 0 0 0 0 0 2 0 1 1 5
Bud Neck 0 0 0 0 1 1 2 5 1 7 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 2 4 5 9 9 9 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 4 2 5 15 19 23 45 18 19 12 16 8 22 40 15 20 5 0 1 0 1 6 2
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 2 0 0 0 0 0 2 2 6 2 0 0 0 0 0 1
Endosome 0 0 1 1 2 2 1 2 0 0 1 0 0 0 1 2 2 0 0 2 2 1 3 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1
Mitochondria 11 112 2 10 3 40 53 56 167 129 115 125 4 4 3 8 14 10 0 5 0 0 2 5
Nucleus 150 172 162 93 140 161 175 191 153 182 130 133 84 142 147 44 100 69 140 177 148 103 123 110
Nuclear Periphery 0 0 0 0 0 1 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 6 0 0 2 0 1 0 0 1 3 2 3 2 3 0 1 6 4 7 7 12 7 20 25
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 4 10 3 12 5 10 18 9 15 5 6 3 5 4 1 2 3 1 3 4 2 5 9
Vac/Vac Membrane 1 2 1 0 3 2 5 1 1 1 0 8 1 1 4 5 3 1 8 22 8 7 15 25
Unique Cell Count 158 214 167 100 163 203 224 282 248 269 189 216 95 168 195 66 129 81 162 226 181 133 184 199
Labelled Cell Count 169 295 178 115 176 234 274 325 366 370 282 309 102 177 201 78 153 94 162 226 181 133 184 199


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 3.8 5.8 5.3 5.8 4.7 4.6 4.8 3.6 4.0 4.2 3.9 5.4 5.5 5.3 5.8 6.2 6.6 6.4 6.5 6.5
Std Deviation (1e-4) 0.7 0.6 0.8 1.4 1.5 1.4 1.5 1.3 0.7 1.0 2.9 1.6 1.1 0.9 1.4 1.8 1.7 1.4 1.4 1.6 1.8
Intensity Change (Log2) – – – -0.13 0.02 -0.28 -0.32 -0.27 -0.68 -0.54 -0.47 -0.55 -0.09 -0.07 -0.11 0.02 0.11 0.19 0.15 0.17 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4232 0.5806 0.2819 -0.0009 0.1562 0.4766 3.7471 3.6707 4.4525 4.3427 4.3701 3.9186 2.0527 2.6233 2.37 2.098 2.2174 2.1283
Actin 0.0017 0.0003 0.0005 0 0.0125 0.0014 0.0033 0 0.0024 0.0001 0.0001 0 0.0014 0.0003 0.004 0.0022 0.0003 0
Bud 0.0005 0.0003 0 0 0.0001 0 0.0007 0 0.0012 0.0001 0 0 0.0002 0.0002 0 0.0001 0.0001 0
Bud Neck 0.0014 0.0003 0.0003 0 0.0004 0.0009 0.0225 0.0003 0.0028 0.0001 0.0009 0.0013 0.0004 0.0003 0.0004 0.0001 0.0004 0.0019
Bud Periphery 0.0004 0.0003 0.0001 0 0.0002 0 0.0019 0 0.002 0.0002 0 0 0.0009 0.0001 0 0.0001 0.0002 0.0001
Bud Site 0.0004 0.0083 0.0001 0 0.0002 0 0.0063 0.0001 0.0082 0.0002 0.0001 0.0001 0.0005 0.0004 0.0003 0.0004 0.0005 0.0001
Cell Periphery 0.0001 0.0001 0 0 0 0 0.0009 0 0.0007 0.0001 0 0 0.0008 0 0 0 0.0001 0
Cytoplasm 0.0074 0.0047 0.0007 0.0002 0.0038 0.003 0.0034 0.0003 0.0076 0.0005 0.0065 0.0046 0.0056 0.0043 0.0013 0.0005 0.0008 0.0004
Cytoplasmic Foci 0.0041 0.0016 0.0001 0 0.0009 0.0005 0.0023 0 0.0017 0.0009 0.0003 0.0001 0.0065 0.0002 0.0046 0.0017 0.0008 0
Eisosomes 0.0001 0 0 0 0.0001 0.0001 0.0004 0 0.0001 0 0.0003 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0018 0.0005 0.0011 0 0.0023 0.0001 0.0008 0 0.0007 0.0009 0.0004 0 0.0006 0.0002 0.0001 0.0002 0.0004 0
Endosome 0.0073 0.0007 0.003 0 0.0084 0.0001 0.0027 0 0.0053 0.0436 0.0004 0 0.0023 0.0001 0.0017 0.0043 0.0148 0
Golgi 0.0013 0.0005 0.0002 0 0.0033 0.0001 0.0008 0 0.0023 0.0067 0 0 0.0006 0 0.0021 0.0022 0.0041 0
Lipid Particles 0.0047 0.0001 0.0002 0 0.0023 0.0002 0.0017 0 0.0053 0.0007 0.0002 0 0.0047 0 0.0011 0.0095 0.0043 0
Mitochondria 0.0013 0.0006 0.0004 0.0001 0.0011 0.0001 0.0044 0.0001 0.0092 0.0065 0.0002 0.0001 0.0013 0.0002 0.0005 0.0122 0.0058 0.0001
None 0.0099 0.0014 0.0026 0 0.0028 0.003 0.032 0 0.0557 0.0001 0.1468 0.0237 0.0013 0.0178 0.0052 0.0006 0.0003 0.0001
Nuclear Periphery 0.0148 0.0183 0.0089 0.0007 0.0207 0.0013 0.0036 0.0008 0.0028 0.0006 0.0035 0.0004 0.0125 0.0033 0.0042 0.0009 0.0042 0.0017
Nucleolus 0.0086 0.0069 0.0065 0.0051 0.0157 0.0189 0.0221 0.0136 0.0104 0.006 0.0218 0.0143 0.0109 0.0096 0.0051 0.0034 0.0231 0.0157
Nucleus 0.9157 0.9301 0.9709 0.9929 0.9174 0.9626 0.88 0.9842 0.8644 0.9211 0.8025 0.9502 0.9096 0.9434 0.964 0.9193 0.9244 0.9562
Peroxisomes 0.0025 0.0001 0 0 0.0002 0 0.0021 0 0.0017 0 0 0 0.0116 0.0001 0.0025 0.0171 0.0004 0
Punctate Nuclear 0.0142 0.024 0.0033 0.0008 0.0066 0.0076 0.005 0.0005 0.0115 0.001 0.0154 0.0049 0.0249 0.0192 0.0018 0.0244 0.011 0.0235
Vacuole 0.0013 0.0007 0.0007 0 0.0004 0.0001 0.0026 0.0001 0.0034 0.0081 0.0004 0.0001 0.0021 0.0001 0.0003 0.0004 0.0023 0.0001
Vacuole Periphery 0.0007 0.0003 0.0004 0 0.0005 0 0.0006 0 0.0007 0.0025 0.0001 0 0.0015 0 0.0006 0.0003 0.0017 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.3398 5.2818 7.8386 11.28 12.4484 10.3341 12.016 14.8683 8.937 15.7847
Translational Efficiency 1.0456 1.5239 0.8483 0.8827 0.7615 1.1297 0.9768 0.7107 1.1557 0.7116

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1711 119 1635 1476 1936 1929 162 1630 3647 2048 1797 3106

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 756.02 844.12 877.65 884.59 728.62 891.76 966.00 905.35 741.47 888.99 885.61 895.48
Standard Deviation 93.40 111.40 104.95 119.96 87.66 107.42 96.88 123.19 91.43 108.24 107.27 122.11
Intensity Change Log 2 — 0.159024 0.215221 0.226585 — 0.291489 0.406857 0.313309 — 0.225554 0.312452 0.269798

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000069 0.001971 0.000691 0.000768 0.000083 0.000211 0.002071 0.001141 0.000076 0.000313 0.000816 0.000964
Bud Neck 0.003578 0.004840 0.011008 0.020482 0.002333 0.005269 0.003503 0.015034 0.002917 0.005244 0.010332 0.017623
Bud Site 0.000478 0.002823 0.002793 0.010191 0.000251 0.001091 0.004347 0.006541 0.000357 0.001191 0.002933 0.008275
Cell Periphery 0.000105 0.000162 0.000086 0.000159 0.000058 0.000085 0.000088 0.000155 0.000080 0.000090 0.000086 0.000157
Cytoplasm 0.040817 0.021396 0.006892 0.038692 0.044757 0.008868 0.002177 0.026341 0.042908 0.009596 0.006467 0.032210
Cytoplasmic Foci 0.003828 0.008252 0.000168 0.003913 0.003218 0.003185 0.000418 0.000994 0.003504 0.003480 0.000191 0.002381
Eisosomes 0.000014 0.000066 0.000025 0.000031 0.000013 0.000020 0.000079 0.000028 0.000013 0.000023 0.000029 0.000029
Endoplasmic Reticulum 0.001266 0.007713 0.004609 0.006749 0.001194 0.002560 0.010166 0.005857 0.001228 0.002860 0.005110 0.006281
Endosome 0.000349 0.004147 0.000406 0.006862 0.000276 0.001150 0.000469 0.001093 0.000310 0.001324 0.000412 0.003835
Golgi 0.000161 0.001299 0.000084 0.003828 0.000103 0.000927 0.000145 0.001341 0.000130 0.000948 0.000090 0.002523
Lipid Particles 0.001022 0.003775 0.000238 0.000944 0.000717 0.001038 0.001139 0.000283 0.000860 0.001197 0.000320 0.000597
Mitochondria 0.000837 0.007853 0.001375 0.003842 0.000784 0.004359 0.000940 0.002150 0.000809 0.004562 0.001336 0.002954
Mitotic Spindle 0.000695 0.005347 0.004045 0.042976 0.001160 0.003928 0.000760 0.012253 0.000942 0.004011 0.003749 0.026853
None 0.010440 0.007760 0.004758 0.009144 0.008479 0.004026 0.001962 0.013075 0.009399 0.004243 0.004506 0.011207
Nuclear Periphery 0.000999 0.000438 0.000713 0.003269 0.000627 0.001113 0.000494 0.001208 0.000801 0.001074 0.000693 0.002188
Nuclear Periphery Foci 0.000271 0.001434 0.000361 0.001240 0.000341 0.000362 0.000479 0.001926 0.000308 0.000425 0.000372 0.001600
Nucleolus 0.009705 0.022974 0.006862 0.004903 0.006546 0.016730 0.008372 0.005325 0.008028 0.017093 0.006998 0.005125
Nucleus 0.924157* 0.886346* 0.952611* 0.823114* 0.927956* 0.941128* 0.953368* 0.899401* 0.926174* 0.937945* 0.952680* 0.863149*
Peroxisomes 0.000418 0.006885 0.000253 0.000532 0.000298 0.001193 0.003231 0.000240 0.000354 0.001523 0.000521 0.000379
Vacuole 0.000646 0.003103 0.001875 0.015234 0.000715 0.002060 0.005648 0.004960 0.000683 0.002121 0.002215 0.009842
Vacuole Periphery 0.000146 0.001417 0.000146 0.003129 0.000091 0.000696 0.000143 0.000652 0.000117 0.000738 0.000145 0.001829

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.97 -13.19 -22.93 2.06 -1.61 -4.73 -5.76 -8.10 -7.16 3.07 -6.11 -13.75 -13.48 -9.46 -0.80
Bud Neck -1.40 -14.10 -14.08 -8.93 -6.36 -5.55 -5.17 -13.20 -10.57 -9.06 -5.03 -15.80 -19.27 -15.48 -7.69
Bud Site -2.86 -4.37 -7.49 -3.05 -3.76 -3.95 -1.65 -6.49 -5.49 0.10 -4.10 -4.74 -9.82 -8.17 -3.56
Cell Periphery -1.55 0.97 -1.94 0.95 -3.57 -1.53 -4.58 -7.73 -2.77 -0.32 -0.58 -2.03 -6.62 -2.58 -4.34
Cytoplasm 2.62 12.10 2.18 -1.54 -10.32 12.85 14.75 6.82 -6.08 -8.36 15.39 18.34 6.35 -9.42 -12.40
Cytoplasmic Foci -1.47 6.10 3.26 2.25 -3.62 0.11 5.42 5.03 3.73 -0.16 0.14 8.78 5.68 3.61 -3.90
Eisosomes -4.47 -10.41 -16.71 1.97 -6.80 -5.55 -8.13 -15.81 -10.37 5.88 -6.90 -13.53 -22.99 -11.25 -2.05
Endoplasmic Reticulum -6.25 -15.78 -17.76 -1.13 -6.91 -7.02 -10.02 -22.82 -15.37 4.93 -8.40 -19.34 -27.50 -17.72 -5.04
Endosome -1.26 -2.70 -4.52 0.36 -4.20 -2.89 -3.36 -3.94 0.65 0.66 -3.04 -4.87 -5.48 -1.33 -4.48
Golgi -2.11 0.76 -2.60 0.17 -2.71 -2.47 -1.66 -1.30 0.27 -1.08 -2.67 0.19 -2.68 -0.24 -2.72
Lipid Particles -1.86 2.30 0.86 2.07 -2.58 -1.05 -2.22 2.50 3.14 3.71 -1.18 2.73 1.87 2.90 -1.45
Mitochondria -1.54 -2.79 -7.51 0.99 -4.83 -4.22 -1.57 -5.16 2.42 -5.18 -4.57 -2.75 -8.93 2.09 -5.13
Mitotic Spindle -1.40 -3.86 -8.67 -4.42 -6.44 -2.12 -1.01 -5.30 -3.46 -3.83 -2.68 -3.79 -10.12 -7.48 -6.14
None 0.97 5.02 2.69 0.23 -2.48 5.51 8.93 -0.51 -3.98 -5.74 6.98 6.00 1.31 -4.49 -4.05
Nuclear Periphery 3.14 0.08 -5.32 -6.53 -5.54 -3.18 -2.43 -8.07 -2.00 -3.36 -1.85 -2.04 -7.03 -5.18 -6.44
Nuclear Periphery Foci -2.33 -1.49 -4.38 0.83 -1.81 -0.17 -2.38 -1.80 -1.75 0.34 -1.25 -1.82 -3.08 -2.55 -1.49
Nucleolus -4.33 0.47 5.45 6.04 4.85 -8.29 -1.78 -0.42 7.88 1.50 -7.78 -1.46 3.68 9.76 4.21
Nucleus 2.20 -3.77 11.04 1.85 14.50 -2.70 0.25 5.76 8.05 2.72 -2.67 -3.85 12.08 13.09 14.90
Peroxisomes -2.71 1.11 1.24 2.78 0.16 -3.16 -1.17 0.82 3.70 1.22 -3.97 -0.63 1.47 4.47 1.28
Vacuole -3.04 -6.41 -11.44 -9.35 -10.26 -2.78 -4.14 -9.04 -7.30 1.53 -3.27 -7.23 -14.36 -12.32 -10.56
Vacuole Periphery -2.01 -0.47 -2.14 0.24 -2.11 -3.42 -5.03 -3.90 1.28 -2.46 -3.64 -2.50 -2.85 -0.26 -2.66
Standard name
Human Ortholog
Description Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Sas5

Sas5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sas5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available