Standard name | |
---|---|
Human Ortholog | |
Description | Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 2 | 10 | 16 | 37 | 39 | 29 | 47 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 0 | 3 | 4 | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 3 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 1 | 4 | 5 | 19 | 28 | 44 | 42 | 45 | 31 | 26 | 46 | 0 | 1 | 10 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 248 | 326 | 416 | 474 | 599 | 536 | 539 | 487 | 424 | 307 | 280 | 294 | 130 | 241 | 307 | 155 | 94 | 107 | 239 | 320 | 384 | 117 | 280 | 242 |
Endoplasmic Reticulum | 0 | 7 | 6 | 7 | 68 | 39 | 138 | 131 | 93 | 102 | 68 | 88 | 6 | 4 | 4 | 6 | 4 | 3 | 3 | 2 | 11 | 1 | 5 | 8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Golgi | 0 | 0 | 0 | 1 | 1 | 4 | 36 | 40 | 16 | 41 | 15 | 46 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 1 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 32 | 13 | 23 | 37 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 1 | 1 | 3 | 5 | 5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 2 | 1 | 1 | 3 | 4 | 9 | 1 | 30 | 23 | 13 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 5 | 2 | 1 | 1 | 2 |
Unique Cell Count | 249 | 331 | 421 | 483 | 659 | 591 | 718 | 666 | 562 | 505 | 410 | 490 | 135 | 244 | 315 | 155 | 94 | 109 | 246 | 338 | 412 | 132 | 307 | 274 |
Labelled Cell Count | 251 | 334 | 430 | 490 | 688 | 613 | 774 | 731 | 652 | 573 | 469 | 578 | 138 | 246 | 326 | 164 | 102 | 112 | 246 | 338 | 412 | 132 | 307 | 274 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 92.5 | 84.1 | 81.5 | 76.0 | 58.7 | 44.3 | 35.2 | 31.3 | 26.6 | 22.9 | 21.0 | 22.0 | 81.1 | 71.3 | 67.1 | 68.5 | 85.3 | 112.5 | 74.5 | 83.7 | 83.1 |
Std Deviation (1e-4) | 16.1 | 20.1 | 17.6 | 13.5 | 10.3 | 7.9 | 7.4 | 6.6 | 5.7 | 6.3 | 5.9 | 5.0 | 22.2 | 18.4 | 18.2 | 26.2 | 34.5 | 33.0 | 28.9 | 29.6 | 31.9 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.47 | -0.88 | -1.21 | -1.38 | -1.61 | -1.83 | -1.96 | -1.89 | -0.01 | -0.19 | -0.28 | -0.25 | 0.07 | 0.46 | -0.13 | 0.04 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 3.2 | 5.4 | 5.8 | 5.6 | 6.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 2.1 | 3.4 | 4.2 | 4.3 | 5.0 | 4.1 | 4.2 | 5.6 | 0 | 0 | 2.2 | 0 | 0 | 0 |
Cytoplasm | -0.8 | -5.3 | -5.4 | -10.5 | -11.0 | -10.3 | -13.9 | -11.9 | -14.1 | -1.9 | 0 | -1.4 | 1.4 | 1.1 | -0.5 |
Endoplasmic Reticulum | 0 | 5.6 | 3.9 | 8.7 | 8.8 | 7.8 | 8.9 | 7.7 | 8.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 4.7 | 5.1 | 3.5 | 6.0 | 4.0 | 6.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 5.0 | 3.3 | 4.9 | 5.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 4.5 | 4.3 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 75.6942 | 104.8212 | 94.4102 | 79.9822 | 77.2264 | 76.1894 | 84.1029 | 113.0717 | 103.2987 | 101.1302 | 87.1415 | 97.6812 | 85.898 | 114.1013 | 102.7388 | 100.338 | 78.7856 | 101.4152 |
Actin | 0.0119 | 0.0004 | 0.018 | 0.0267 | 0.0007 | 0.0001 | 0.0175 | 0.0002 | 0.0004 | 0.0002 | 0.0005 | 0.0009 | 0.024 | 0.0007 | 0.0023 | 0.0005 | 0.0006 | 0.0016 |
Bud | 0.003 | 0.0005 | 0.0021 | 0.0019 | 0.0019 | 0.0001 | 0.0034 | 0.0002 | 0.0015 | 0.0006 | 0.0016 | 0.0003 | 0.0022 | 0.0003 | 0.0008 | 0.0019 | 0.0006 | 0.0006 |
Bud Neck | 0.0009 | 0.0004 | 0.0006 | 0.0008 | 0.0021 | 0.0008 | 0.0008 | 0.0002 | 0.0004 | 0.0005 | 0.0006 | 0.0005 | 0.0009 | 0.0002 | 0.0006 | 0.0007 | 0.0004 | 0.0011 |
Bud Periphery | 0.009 | 0.0003 | 0.0018 | 0.0009 | 0.0009 | 0 | 0.0032 | 0.0001 | 0.0015 | 0.0001 | 0.0007 | 0.0002 | 0.0025 | 0.0002 | 0.0004 | 0.0009 | 0.0003 | 0.0002 |
Bud Site | 0.0022 | 0.0002 | 0.0028 | 0.0012 | 0.001 | 0 | 0.0015 | 0.0001 | 0.0003 | 0 | 0.0008 | 0.0004 | 0.0009 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0001 |
Cell Periphery | 0.0167 | 0.0057 | 0.0031 | 0.0026 | 0.0042 | 0 | 0.0044 | 0.0007 | 0.0013 | 0.0005 | 0.0014 | 0.0003 | 0.0054 | 0.0013 | 0.0041 | 0.0065 | 0.0017 | 0.0004 |
Cytoplasm | 0.6016 | 0.94 | 0.838 | 0.5125 | 0.7495 | 0.9776 | 0.7691 | 0.9855 | 0.9209 | 0.9116 | 0.7296 | 0.9018 | 0.7267 | 0.9748 | 0.9028 | 0.8171 | 0.849 | 0.9026 |
Cytoplasmic Foci | 0.0166 | 0.0007 | 0.0084 | 0.0152 | 0.0064 | 0.0036 | 0.0162 | 0.0007 | 0.0046 | 0.0016 | 0.0464 | 0.0132 | 0.005 | 0.0007 | 0.0064 | 0.0027 | 0.007 | 0.0023 |
Eisosomes | 0.0005 | 0 | 0.0006 | 0.0004 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0.0011 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0647 | 0.0166 | 0.0075 | 0.0053 | 0.0043 | 0.0025 | 0.0306 | 0.0026 | 0.0108 | 0.0078 | 0.0284 | 0.0136 | 0.0391 | 0.0051 | 0.007 | 0.0055 | 0.0041 | 0.0641 |
Endosome | 0.0238 | 0.0012 | 0.0073 | 0.0242 | 0.0254 | 0.0066 | 0.0158 | 0.001 | 0.0063 | 0.007 | 0.0129 | 0.0102 | 0.0059 | 0.0012 | 0.0035 | 0.0062 | 0.0166 | 0.0053 |
Golgi | 0.0068 | 0.0002 | 0.004 | 0.0102 | 0.0009 | 0.0004 | 0.0031 | 0.0001 | 0.0006 | 0.0004 | 0.0006 | 0.0009 | 0.0015 | 0.0003 | 0.0007 | 0.0004 | 0.0098 | 0.0007 |
Lipid Particles | 0.0065 | 0.0001 | 0.0039 | 0.0181 | 0.0012 | 0 | 0.0048 | 0.0001 | 0.0012 | 0.0002 | 0.0048 | 0.0046 | 0.0018 | 0.0003 | 0.0026 | 0.0015 | 0.0088 | 0.0001 |
Mitochondria | 0.0156 | 0.0003 | 0.0088 | 0.0309 | 0.0066 | 0.0001 | 0.0078 | 0.0001 | 0.0043 | 0.0002 | 0.0162 | 0.037 | 0.0056 | 0.0004 | 0.0078 | 0.0057 | 0.0294 | 0.0007 |
None | 0.0054 | 0.0001 | 0.0039 | 0.0041 | 0.0037 | 0.0001 | 0.0094 | 0.0001 | 0.0046 | 0.0002 | 0.0288 | 0.003 | 0.0039 | 0.0002 | 0.0051 | 0.0009 | 0.003 | 0.0003 |
Nuclear Periphery | 0.0433 | 0.0087 | 0.011 | 0.0117 | 0.0136 | 0.0012 | 0.0278 | 0.001 | 0.004 | 0.0097 | 0.0052 | 0.001 | 0.0314 | 0.0034 | 0.0054 | 0.0075 | 0.0044 | 0.0107 |
Nucleolus | 0.0082 | 0 | 0.0022 | 0.0008 | 0.0224 | 0 | 0.0019 | 0 | 0.0009 | 0 | 0.0069 | 0.0024 | 0.0052 | 0.0002 | 0.0035 | 0.0015 | 0.0127 | 0 |
Nucleus | 0.0128 | 0.0015 | 0.0045 | 0.0047 | 0.0115 | 0.0009 | 0.0084 | 0.0007 | 0.0022 | 0.0026 | 0.0047 | 0.001 | 0.0134 | 0.0014 | 0.0038 | 0.0052 | 0.0084 | 0.0021 |
Peroxisomes | 0.0015 | 0 | 0.0066 | 0.0111 | 0.0003 | 0 | 0.0011 | 0 | 0.0002 | 0 | 0.0017 | 0.0025 | 0.0022 | 0 | 0.0004 | 0.0002 | 0.0016 | 0 |
Punctate Nuclear | 0.0029 | 0 | 0.0007 | 0.0018 | 0.0003 | 0 | 0.0065 | 0 | 0.0004 | 0 | 0.0027 | 0.0002 | 0.0019 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Vacuole | 0.1047 | 0.0195 | 0.049 | 0.2148 | 0.0979 | 0.0037 | 0.051 | 0.006 | 0.0273 | 0.0458 | 0.0733 | 0.0029 | 0.0975 | 0.0077 | 0.0342 | 0.1091 | 0.0319 | 0.0049 |
Vacuole Periphery | 0.0415 | 0.0035 | 0.0151 | 0.1001 | 0.0449 | 0.0022 | 0.0154 | 0.0007 | 0.006 | 0.0111 | 0.032 | 0.0019 | 0.0227 | 0.001 | 0.0078 | 0.0252 | 0.009 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 955.2622 | 974.1987 | 903.6218 | 1027.4148 | 993.4806 | 701.1589 | 778.005 | 790.5081 | 720.6267 | 857.8483 |
Translational Efficiency | 2.0644 | 2.1278 | 2.3674 | 1.6413 | 2.1434 | 2.6596 | 2.2503 | 2.3597 | 2.318 | 2.1013 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
201 | 1353 | 2794 | 1531 | 2136 | 2467 | 3439 | 1549 | 2337 | 3820 | 6233 | 3080 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 3904.95 | 5354.79 | 8980.79 | 6447.51 | 4518.61 | 5205.48 | 8282.64 | 6843.53 | 4465.83 | 5258.36 | 8595.59 | 6646.68 |
Standard Deviation | 859.22 | 1160.70 | 1543.80 | 2627.80 | 1066.27 | 1248.39 | 1858.01 | 2573.00 | 1064.07 | 1220.15 | 1758.87 | 2607.91 |
Intensity Change Log 2 | — | 0.455526 | 1.201538 | 0.723438 | — | 0.204152 | 0.874212 | 0.598862 | — | 0.326145 | 1.035217 | 0.657952 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000141 | 0.000059 | 0.000062 | 0.000434 | 0.000038 | 0.000041 | 0.000038 | 0.000228 | 0.000046 | 0.000048 | 0.000049 | 0.000330 |
Bud Neck | 0.000801 | 0.000517 | 0.000345 | 0.002615 | 0.000131 | 0.000252 | 0.000292 | 0.001606 | 0.000188 | 0.000346 | 0.000316 | 0.002108 |
Bud Site | 0.008980 | 0.003298 | 0.002284 | 0.016884 | 0.001308 | 0.001774 | 0.003746 | 0.011494 | 0.001968 | 0.002314 | 0.003090 | 0.014173 |
Cell Periphery | 0.032355 | 0.013000 | 0.019901 | 0.045868 | 0.008178 | 0.003475 | 0.005529 | 0.030366 | 0.010257 | 0.006849 | 0.011971 | 0.038071 |
Cytoplasm | 0.328712* | 0.720603* | 0.748081* | 0.609340* | 0.731394* | 0.814002* | 0.754984* | 0.685267* | 0.696760* | 0.780921* | 0.751890* | 0.647526* |
Cytoplasmic Foci | 0.023163 | 0.009995 | 0.003558 | 0.011926 | 0.009395 | 0.007233 | 0.003567 | 0.010024 | 0.010579 | 0.008211 | 0.003563 | 0.010969 |
Eisosomes | 0.000003 | 0.000001 | 0.000001 | 0.000008 | 0.000001 | 0.000001 | 0.000001 | 0.000003 | 0.000001 | 0.000001 | 0.000001 | 0.000005 |
Endoplasmic Reticulum | 0.002321 | 0.001292 | 0.001232 | 0.001016 | 0.002041 | 0.000699 | 0.000595 | 0.000362 | 0.002065 | 0.000909 | 0.000881 | 0.000687 |
Endosome | 0.002569 | 0.000712 | 0.000429 | 0.001207 | 0.000636 | 0.000618 | 0.000463 | 0.000548 | 0.000802 | 0.000651 | 0.000448 | 0.000876 |
Golgi | 0.284846* | 0.069665 | 0.016119 | 0.012388 | 0.048154 | 0.035786 | 0.007332 | 0.013550 | 0.068511 | 0.047786 | 0.011271 | 0.012972 |
Lipid Particles | 0.023904 | 0.013679 | 0.015672 | 0.028923 | 0.006547 | 0.004400 | 0.004208 | 0.019597 | 0.008040 | 0.007687 | 0.009347 | 0.024232 |
Mitochondria | 0.000234 | 0.000162 | 0.000244 | 0.001313 | 0.000217 | 0.000233 | 0.000303 | 0.000339 | 0.000219 | 0.000208 | 0.000277 | 0.000823 |
Mitotic Spindle | 0.002749 | 0.001122 | 0.000364 | 0.013673 | 0.000502 | 0.001046 | 0.000290 | 0.005382 | 0.000695 | 0.001073 | 0.000323 | 0.009503 |
None | 0.001504 | 0.001633 | 0.000694 | 0.002817 | 0.002679 | 0.002580 | 0.000603 | 0.001724 | 0.002578 | 0.002245 | 0.000644 | 0.002267 |
Nuclear Periphery | 0.000678 | 0.000657 | 0.000459 | 0.000535 | 0.000416 | 0.000299 | 0.000102 | 0.000454 | 0.000438 | 0.000426 | 0.000262 | 0.000494 |
Nuclear Periphery Foci | 0.019989 | 0.016928 | 0.006236 | 0.007429 | 0.010816 | 0.010124 | 0.002181 | 0.006883 | 0.011605 | 0.012534 | 0.003999 | 0.007155 |
Nucleolus | 0.000144 | 0.000065 | 0.000071 | 0.000260 | 0.000068 | 0.000097 | 0.000122 | 0.000182 | 0.000075 | 0.000086 | 0.000099 | 0.000221 |
Nucleus | 0.002739 | 0.005012 | 0.003958 | 0.017245 | 0.002545 | 0.005226 | 0.003485 | 0.017822 | 0.002562 | 0.005150 | 0.003697 | 0.017535 |
Peroxisomes | 0.000034 | 0.000031 | 0.000073 | 0.000169 | 0.000026 | 0.000016 | 0.000093 | 0.000113 | 0.000027 | 0.000021 | 0.000084 | 0.000141 |
Vacuole | 0.081306 | 0.074282 | 0.135873 | 0.200048* | 0.060628 | 0.055283 | 0.171080 | 0.169824 | 0.062406 | 0.062012 | 0.155298 | 0.184847 |
Vacuole Periphery | 0.182825* | 0.067287 | 0.044344 | 0.025901 | 0.114283* | 0.056815 | 0.040985 | 0.024232 | 0.120178* | 0.060524 | 0.042491 | 0.025062 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.60 | 2.29 | -5.05 | -6.75 | -6.60 | -0.31 | -0.95 | -5.86 | -5.84 | -5.75 | -0.10 | -1.24 | -8.87 | -8.95 | -8.75 |
Bud Neck | 1.07 | 1.49 | -8.20 | -10.45 | -10.76 | -1.94 | -5.37 | -9.97 | -9.46 | -9.02 | -3.24 | -5.45 | -15.04 | -14.22 | -13.99 |
Bud Site | 2.72 | 3.17 | -5.53 | -11.55 | -12.35 | -2.34 | -9.18 | -11.30 | -10.91 | -8.93 | -1.18 | -5.21 | -16.42 | -16.12 | -15.10 |
Cell Periphery | 4.34 | 2.76 | -0.98 | -10.78 | -7.56 | 8.03 | 4.57 | -10.91 | -14.36 | -13.05 | 4.84 | -2.30 | -14.73 | -18.33 | -14.25 |
Cytoplasm | -15.81 | -17.32 | -9.75 | 12.00 | 16.22 | -9.77 | -2.20 | 8.21 | 17.10 | 10.93 | -10.19 | -6.37 | 9.89 | 22.43 | 19.39 |
Cytoplasmic Foci | 4.82 | 7.50 | -1.13 | -7.17 | -10.38 | 2.93 | 9.64 | -6.69 | -7.74 | -9.56 | 3.26 | 11.78 | -9.15 | -10.85 | -14.12 |
Eisosomes | 2.93 | 2.62 | -2.88 | -4.46 | -4.32 | 1.82 | -0.94 | -9.03 | -9.86 | -8.61 | 1.35 | -1.49 | -6.55 | -6.81 | -6.33 |
Endoplasmic Reticulum | 1.63 | 2.28 | 2.75 | 0.90 | 1.45 | 8.03 | 9.26 | 10.90 | 5.00 | 5.13 | 5.40 | 7.88 | 7.65 | 1.62 | 2.15 |
Endosome | 6.92 | 8.00 | 3.91 | -4.19 | -6.05 | 0.26 | 3.60 | -2.08 | -2.26 | -5.47 | 2.73 | 7.25 | -2.93 | -4.86 | -7.49 |
Golgi | 13.22 | 16.78 | 17.11 | 19.84 | 6.37 | 5.39 | 22.47 | 18.72 | 14.92 | -6.91 | 7.11 | 22.36 | 22.06 | 24.53 | -0.53 |
Lipid Particles | 2.90 | 2.29 | -2.11 | -8.50 | -7.81 | 3.66 | 3.81 | -10.68 | -12.31 | -12.38 | 0.43 | -2.45 | -14.83 | -15.53 | -14.19 |
Mitochondria | 0.64 | -0.20 | -1.54 | -1.68 | -1.53 | -0.36 | -3.60 | -2.16 | -1.79 | -1.13 | 0.26 | -2.73 | -1.89 | -1.92 | -1.67 |
Mitotic Spindle | 4.90 | 7.64 | -8.61 | -9.13 | -9.37 | -1.92 | 0.19 | -6.92 | -6.57 | -6.92 | -1.99 | 2.54 | -11.43 | -11.17 | -11.56 |
None | -0.86 | 5.93 | -2.49 | -2.26 | -4.26 | 0.13 | 3.33 | 1.15 | 1.19 | -6.13 | 0.52 | 3.41 | 0.42 | -0.19 | -6.45 |
Nuclear Periphery | 0.08 | 2.46 | -0.41 | -0.61 | -3.78 | 2.67 | 11.59 | -5.04 | -6.70 | -13.76 | 0.12 | 5.45 | -6.30 | -6.02 | -11.76 |
Nuclear Periphery Foci | 1.35 | 7.16 | 5.45 | 6.42 | -3.35 | 0.72 | 13.25 | 2.13 | 1.48 | -10.70 | -1.31 | 11.99 | 3.37 | 5.14 | -9.77 |
Nucleolus | 0.98 | 0.87 | -1.77 | -6.98 | -6.75 | -0.95 | -4.53 | -6.88 | -4.11 | -5.10 | -0.52 | -2.16 | -8.36 | -6.80 | -8.33 |
Nucleus | -3.45 | -2.70 | -13.16 | -12.23 | -12.55 | -5.35 | -5.20 | -15.82 | -13.31 | -14.19 | -6.99 | -6.53 | -20.72 | -17.81 | -18.75 |
Peroxisomes | 0.31 | -3.22 | -5.84 | -6.49 | -4.48 | 2.24 | -9.28 | -5.60 | -6.34 | -1.60 | 1.30 | -10.00 | -8.60 | -9.22 | -4.42 |
Vacuole | 0.71 | -6.66 | -11.48 | -17.73 | -7.98 | 1.41 | -26.05 | -18.45 | -19.22 | 0.33 | -0.16 | -28.05 | -26.34 | -27.01 | -5.11 |
Vacuole Periphery | 8.22 | 10.06 | 11.76 | 13.98 | 12.49 | 13.75 | 19.78 | 25.11 | 14.61 | 12.19* | 15.25 | 21.73 | 27.78 | 20.09 | 17.41 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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