ORF
Human Ortholog
Description Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.1 0.06 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.99 0.99 0.99 0.97 0.93 0.96 0.85 – 0.75 0.84 1.0 1.0 1.0 0.9 0.89 0.96 0.88 0.94 0.9 0.76 0.77 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.12 0.08 0.35 – 0.43 0.25 0 0 0 0 0 0 0 0 0 0.1 0.07 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0.09 0 0 0 0 0.05 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 2 2 0 0 2 2
Bud 0 1 0 0 1 4 9 3 16 – 10 12 1 1 1 0 0 0 1 0 0 2 5 9
Bud Neck 0 0 3 1 1 2 3 11 1 – 1 1 0 0 0 0 0 0 0 0 0 1 3 2
Bud Site 0 0 0 0 0 0 0 0 1 – 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 4 5 5 3 3 3 5 – 4 4 0 3 5 15 31 14 0 0 0 0 0 0
Cytoplasm 164 294 323 253 304 282 477 369 354 – 225 242 201 219 230 266 276 222 153 292 294 112 183 134
Endoplasmic Reticulum 3 4 1 2 0 0 0 0 0 – 1 1 0 1 0 10 7 8 2 1 2 4 6 2
Endosome 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 4 2 0 0 3 0 0 1 0
Golgi 0 1 0 0 1 0 0 0 0 – 0 0 0 0 0 2 3 1 1 0 0 0 0 0
Mitochondria 5 7 0 4 8 28 61 30 146 – 130 73 2 2 4 4 2 3 0 5 14 14 16 7
Nucleus 1 0 0 2 1 3 7 8 7 – 3 0 0 0 0 2 4 3 1 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 3 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 1 0 0 – 0 0 0 0 0 0 2 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 – 1 4 0 0 0 0 0 0 0 0 1 0 0 1
Vac/Vac Membrane 0 1 0 0 2 1 1 1 2 – 1 3 0 0 1 23 27 8 6 2 3 7 13 7
Unique Cell Count 170 306 325 255 308 291 511 386 415 302 289 202 219 231 295 309 231 175 312 327 148 238 173
Labelled Cell Count 174 310 331 267 323 323 564 425 532 376 341 204 226 241 329 354 259 175 312 327 148 238 173


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 4.7 5.3 5.1 5.6 4.4 4.7 4.9 3.9 – 3.7 4.2 5.2 5.3 5.6 9.4 9.3 8.8 5.3 5.0 5.8
Std Deviation (1e-4) 1.1 0.6 0.9 1.7 1.8 1.4 1.6 1.6 1.0 – 1.0 1.0 1.4 1.0 1.6 1.3 1.4 1.2 2.4 1.7 2.1
Intensity Change (Log2) – – – -0.04 0.07 -0.28 -0.16 -0.1 -0.43 – -0.51 -0.33 -0.01 0.01 0.08 0.84 0.82 0.73 0.01 -0.08 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 5.1 5.4 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7092 2.5558 2.2414 1.7273 1.9463 2.3498 0.8861 1.5359 1.0084 0.915 1.2747 0.9907 0.9179 1.4793 1.2178 1.0444 1.128 1.0852
Actin 0.0469 0.0003 0.0042 0.0012 0.031 0.0044 0.0331 0.0035 0.016 0.0216 0.0211 0.0075 0.0175 0.0014 0.0119 0.0242 0.0336 0.0069
Bud 0.0009 0.0004 0.0019 0.0003 0.0005 0.0003 0.0022 0.0157 0.0077 0.0395 0.0009 0.0021 0.0292 0.0197 0.0408 0.0268 0.0393 0.024
Bud Neck 0.0066 0.0003 0.0106 0.001 0.002 0.0003 0.0009 0.0004 0.0022 0.0023 0.0021 0.0011 0.0043 0.0005 0.0008 0.0014 0.0012 0.0051
Bud Periphery 0.0012 0.0004 0.005 0.0005 0.0006 0.002 0.0036 0.0077 0.0073 0.0284 0.0016 0.0028 0.0176 0.008 0.0204 0.0214 0.0294 0.0187
Bud Site 0.0222 0.0042 0.002 0.0003 0.0134 0.0001 0.0094 0.0318 0.0136 0.0249 0.002 0.0022 0.0375 0.0331 0.0348 0.0075 0.0062 0.0019
Cell Periphery 0.001 0.0009 0.001 0.0004 0.0003 0.0001 0.0009 0.001 0.0007 0.0007 0.0003 0.0003 0.002 0.0011 0.0014 0.0006 0.0006 0.0006
Cytoplasm 0.3549 0.5631 0.4547 0.5069 0.549 0.6078 0.5173 0.6207 0.6068 0.4899 0.5318 0.67 0.3767 0.5895 0.4193 0.4182 0.4369 0.4839
Cytoplasmic Foci 0.0378 0.0058 0.0152 0.0105 0.021 0.0041 0.0192 0.0175 0.0307 0.0325 0.0233 0.0188 0.0378 0.0117 0.0153 0.0289 0.0343 0.0149
Eisosomes 0.0006 0.0002 0.0004 0.0001 0.0004 0.0001 0.0004 0.0001 0.0001 0.0001 0.0008 0.0001 0.0003 0.0001 0.0002 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0045 0.0015 0.0039 0.0041 0.0016 0.0024 0.0047 0.0022 0.0038 0.0017 0.0061 0.0014 0.0044 0.0016 0.0023 0.0034 0.0042 0.0018
Endosome 0.026 0.0015 0.0096 0.0021 0.0046 0.0067 0.0189 0.009 0.0234 0.0328 0.055 0.0068 0.0161 0.0038 0.0063 0.0214 0.023 0.0027
Golgi 0.0077 0.0002 0.002 0.0003 0.0041 0.003 0.0144 0.0014 0.0023 0.0094 0.019 0.0018 0.0036 0.0003 0.0015 0.0024 0.0068 0.0003
Lipid Particles 0.0211 0.0002 0.0034 0.0002 0.0047 0.0072 0.0121 0.0018 0.0007 0.0028 0.0061 0.0011 0.0043 0.0003 0.0009 0.0043 0.0242 0.0002
Mitochondria 0.0048 0.0001 0.0013 0.0002 0.0025 0.0079 0.0149 0.0027 0.0016 0.0042 0.0462 0.0049 0.0025 0.0009 0.0015 0.0027 0.0069 0.0008
None 0.4285 0.4176 0.4631 0.4688 0.3444 0.3463 0.3156 0.2719 0.2658 0.271 0.2453 0.2654 0.4132 0.3097 0.4068 0.3936 0.3109 0.4122
Nuclear Periphery 0.0017 0.0002 0.0019 0.0004 0.0002 0.0011 0.0052 0.0007 0.0014 0.001 0.0126 0.0007 0.0043 0.0014 0.0043 0.0027 0.0065 0.0023
Nucleolus 0.0057 0.0001 0.0004 0.0001 0.0002 0 0.0007 0.0007 0.0003 0.0034 0.0013 0.0002 0.0018 0.0012 0.0017 0.0017 0.0024 0.0007
Nucleus 0.0028 0.0006 0.0141 0.0006 0.0003 0.0003 0.0021 0.0028 0.0021 0.0069 0.0045 0.0024 0.0047 0.0066 0.0118 0.0057 0.007 0.0094
Peroxisomes 0.0143 0.0001 0.0014 0.0002 0.0177 0.0023 0.0167 0.0028 0.004 0.0165 0.004 0.0046 0.0051 0.0005 0.0009 0.0166 0.0079 0.0004
Punctate Nuclear 0.0058 0.0002 0.0011 0.0005 0.0007 0.0003 0.0011 0.0009 0.0023 0.003 0.0082 0.0038 0.0118 0.005 0.0137 0.0112 0.0102 0.0103
Vacuole 0.0043 0.0021 0.0025 0.0014 0.0006 0.0016 0.0043 0.0041 0.0061 0.0064 0.0051 0.0016 0.0046 0.0032 0.0029 0.0042 0.0065 0.0023
Vacuole Periphery 0.0006 0.0001 0.0005 0.0001 0.0001 0.0015 0.0025 0.0007 0.001 0.0009 0.0027 0.0003 0.0007 0.0004 0.0005 0.001 0.0019 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.3784 4.808 6.3121 8.1587 4.5595 9.0969 7.1985 8.9599 8.6294 6.8627
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1820 360 1420 1543 2222 2544 146 740 4042 2904 1566 2283

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 620.45 820.02 1006.22 874.05 705.30 762.14 967.86 814.49 667.09 769.32 1002.64 854.74
Standard Deviation 68.82 114.01 131.01 111.65 77.81 124.67 103.35 129.03 85.10 124.87 129.17 120.82
Intensity Change Log 2 — 0.402344 0.697559 0.494401 — 0.111819 0.456561 0.207660 — 0.255087 0.574372 0.348968

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000060 0.000455 0.000436 0.002685 0.000137 0.000723 0.000350 0.000486 0.000102 0.000690 0.000428 0.001973
Bud Neck 0.008866 0.019220 0.002536 0.011677 0.019323 0.042822 0.002805 0.004983 0.014615 0.039896 0.002561 0.009507
Bud Site 0.003627 0.027878 0.017288 0.084040 0.007386 0.034946 0.005645 0.086379 0.005694 0.034070 0.016203 0.084798
Cell Periphery 0.000119 0.000147 0.000097 0.000191 0.000118 0.000187 0.000210 0.000146 0.000119 0.000182 0.000108 0.000176
Cytoplasm 0.652481* 0.435899* 0.659806* 0.525581* 0.524843* 0.478254* 0.443503* 0.635935* 0.582315* 0.473004* 0.639640* 0.561350*
Cytoplasmic Foci 0.143690 0.271975* 0.020350 0.023702 0.258164* 0.211746* 0.031098 0.044018 0.206620* 0.219213* 0.021352 0.030287
Eisosomes 0.000141 0.000126 0.000030 0.000038 0.000170 0.000155 0.000107 0.000035 0.000156 0.000151 0.000037 0.000037
Endoplasmic Reticulum 0.000312 0.000688 0.001628 0.001313 0.000968 0.000500 0.004032 0.000470 0.000673 0.000524 0.001853 0.001040
Endosome 0.003870 0.045300 0.004184 0.007899 0.017864 0.038225 0.006318 0.003795 0.011563 0.039102 0.004383 0.006569
Golgi 0.001176 0.008357 0.000286 0.002911 0.005839 0.011209 0.000105 0.000243 0.003739 0.010856 0.000270 0.002046
Lipid Particles 0.003685 0.005531 0.001375 0.001094 0.005568 0.002807 0.003998 0.000654 0.004720 0.003144 0.001619 0.000951
Mitochondria 0.001331 0.004244 0.000314 0.005573 0.004675 0.009186 0.000203 0.000353 0.003169 0.008574 0.000303 0.003881
Mitotic Spindle 0.000283 0.002796 0.009806 0.074311 0.000867 0.009709 0.001528 0.011468 0.000604 0.008852 0.009035 0.053941
None 0.005596 0.000514 0.004483 0.006343 0.001919 0.001935 0.001911 0.004696 0.003575 0.001759 0.004243 0.005809
Nuclear Periphery 0.000223 0.000188 0.000994 0.000907 0.000141 0.000150 0.001578 0.000316 0.000178 0.000155 0.001049 0.000715
Nuclear Periphery Foci 0.000164 0.000686 0.002639 0.000994 0.000220 0.000172 0.003349 0.001364 0.000195 0.000235 0.002705 0.001114
Nucleolus 0.000699 0.000572 0.000193 0.000473 0.000513 0.000620 0.000382 0.000241 0.000597 0.000614 0.000211 0.000398
Nucleus 0.136362* 0.027622 0.152473* 0.103712 0.062661 0.028754 0.232009* 0.051269 0.095847 0.028614 0.159888* 0.086714
Peroxisomes 0.001117 0.012386 0.000413 0.003821 0.004160 0.009485 0.000438 0.002083 0.002790 0.009845 0.000415 0.003258
Vacuole 0.035764 0.131595 0.120443 0.140059 0.082654 0.114960 0.260241* 0.150854 0.061541 0.117022 0.133477 0.143558
Vacuole Periphery 0.000433 0.003821 0.000227 0.002672 0.001811 0.003454 0.000190 0.000213 0.001191 0.003500 0.000224 0.001875

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.83 -3.51 -3.90 -2.25 -2.18 -3.09 -4.73 -4.29 0.95 -0.57 -3.55 -3.45 -4.47 -0.75 -2.15
Bud Neck -4.16 6.97 0.87 4.71 -7.65 -11.66 8.86 11.40 20.54 -0.94 -14.98 15.92 9.02 20.55 -7.46
Bud Site -7.65 -5.03 -10.36 -1.75 -6.25 -16.67 0.32 -7.11 -2.13 -6.52 -19.19 -3.97 -12.14 -1.55 -8.33
Cell Periphery -1.22 3.66 0.76 1.50 -0.99 -2.24 -3.07 0.78 2.58 3.49 -2.40 1.74 0.97 2.70 -0.25
Cytoplasm 14.15 11.41 23.35 -0.86 9.75 5.80 9.87 1.74 -1.86 -8.06 16.50 6.72 16.01 0.95 6.38
Cytoplasmic Foci -10.62 35.18 34.80 22.47 -0.71 7.97 45.61 41.30 34.44 -5.20 -2.27 60.13 55.96 48.55 -5.57
Eisosomes 1.07 14.25 13.49 5.16 -3.02 0.92 4.22 17.21 6.76 8.32 0.48 20.96 22.08 7.36 0.94
Endoplasmic Reticulum -1.84 -15.00 -15.29 -4.02 5.12 2.31 -7.04 1.69 -1.40 7.53 0.96 -12.76 -6.75 -5.07 9.24
Endosome -9.90 0.31 0.69 9.96 0.43 -11.84 12.07 10.97 19.55 0.55 -18.47 13.97 12.34 24.19 -0.26
Golgi -3.92 6.19 1.00 4.07 -1.96 -7.92 16.23 15.85 19.30 -3.20 -12.10 16.52 10.41 17.43 -2.00
Lipid Particles -0.80 4.94 5.31 2.00 1.05 6.57 2.97 11.62 7.33 5.27 3.93 10.03 11.94 5.86 3.51
Mitochondria -2.17 6.59 -1.54 1.53 -3.98 -4.68 6.79 6.78 12.77 -0.17 -7.34 7.80 3.70 10.21 -3.91
Mitotic Spindle -3.70 -5.15 -11.91 -11.00 -8.55 -6.15 -1.50 -4.80 -1.49 -3.90 -6.77 -5.02 -12.68 -8.73 -8.33
None 7.16 2.50 0.74 -7.62 -1.94 -0.15 1.11 -2.14 -2.01 -2.77 3.95 0.31 -1.54 -5.09 -1.85
Nuclear Periphery 0.50 -19.80 -19.71 -19.64 -4.26 -0.58 -14.08 -11.54 -10.53 9.43 0.71 -25.81 -22.89 -23.03 0.53
Nuclear Periphery Foci -1.98 -15.23 -15.15 -2.00 9.40 1.07 -8.05 -4.47 -4.57 2.39 -0.84 -16.59 -8.32 -7.90 6.46
Nucleolus 0.72 2.37 0.60 -0.28 -4.13 -2.03 4.26 2.70 4.02 -1.14 0.14 3.79 0.75 0.99 -4.58
Nucleus 24.91 -9.34 -3.27 -23.36 5.51 15.90 -9.65 0.48 -9.02 9.63 31.45 -19.53 -7.54 -27.04 12.25
Peroxisomes -7.40 7.26 -0.73 7.04 -2.97 -8.21 9.70 3.20 10.14 -3.60 -13.10 11.15 3.25 14.36 -4.49
Vacuole -12.21 -25.29 -33.53 -11.45 -7.66 -9.02 -12.90 -20.00 -16.87 3.32 -18.61 -24.04 -36.57 -25.24 -7.57
Vacuole Periphery -2.11 6.14 -2.08 1.86 -3.50 -7.65 13.38 13.44 18.64 0.38 -9.01 14.14 4.42 10.68 -3.43
ORF
Human Ortholog
Description Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (90%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YJL070C

YJL070C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yjl070c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available