Standard name
Human Ortholog
Description Rhodanese domain sulfur transferase; accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.97 0.94 0.93 0.93 – 0.73 0.74 0.65 0.67 0.99 0.99 1.0 0.94 0.96 0.91 0.87 0.85 0.77 0.8 0.82 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.1 0.07 0.07 – 0.42 0.37 0.44 0.46 0 0 0 0 0 0 0.07 0.07 0.13 0.1 0.05 0.11
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 – 0 0 0 2 1 0 0 1 0 1 0 2 2 3 2 4
Bud 1 1 1 1 4 1 1 – 6 2 5 3 0 1 0 0 0 0 0 1 6 7 8 5
Bud Neck 0 1 7 4 5 2 2 – 4 4 1 1 0 3 0 0 0 0 0 0 1 2 2 4
Bud Site 0 0 0 1 0 0 0 – 2 2 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 3 3 0 2 2 – 1 0 1 1 0 0 1 5 5 1 0 0 4 3 1 3
Cytoplasm 158 268 375 269 212 338 338 – 168 148 192 244 268 425 333 268 208 262 152 242 278 340 299 213
Endoplasmic Reticulum 2 1 0 1 1 1 1 – 0 0 0 0 0 0 1 18 7 13 0 0 3 3 1 2
Endosome 0 0 1 0 0 3 2 – 1 1 0 1 0 0 0 7 0 4 3 3 3 2 2 3
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 1 0 0 0 2 0 0
Mitochondria 3 6 0 7 22 25 25 – 96 74 132 166 0 1 0 9 3 2 12 19 45 40 18 31
Nucleus 0 0 2 0 3 1 1 – 3 3 4 1 0 0 0 2 9 8 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 1 0 0 – 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 4 4 0 5 4 – 4 5 10 11 0 2 0 0 0 1 0 4 4 5 8 3
Vac/Vac Membrane 0 0 1 0 0 5 3 – 1 2 1 5 0 1 0 18 4 20 0 3 2 6 7 6
Unique Cell Count 161 272 385 277 226 365 365 230 201 297 364 270 430 334 285 217 287 176 284 363 426 364 289
Labelled Cell Count 164 278 396 290 248 383 379 287 242 348 438 270 433 335 329 236 313 176 284 363 426 364 289


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.4 4.9 4.5 4.1 3.9 3.9 – 4.3 3.6 3.5 3.5 5.0 4.5 4.6 6.7 8.0 7.6 5.5 5.4 5.6
Std Deviation (1e-4) 0.5 0.7 1.2 1.2 1.4 1.4 1.4 – 10.5 1.3 1.3 1.2 1.4 1.1 1.4 1.3 1.5 1.6 2.0 1.7 1.7
Intensity Change (Log2) – – – -0.12 -0.26 -0.34 -0.34 – -0.2 -0.45 -0.47 -0.49 0.02 -0.12 -0.09 0.46 0.7 0.63 0.16 0.15 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – 0
Bud Neck – – – – – – – – – – – – 0 – 0
Bud Site – – – – – – – – – – – – 0 – 0
Cell Periphery – – – – – – – – – – – – 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – 0
Endosome – – – – – – – – – – – – 0 – 0
Golgi – – – – – – – – – – – – 0 – 0
Mitochondria – – – – – – – – – – – – 0 – 0
Nuclear Periphery – – – – – – – – – – – – 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – 0
Peroxisomes – – – – – – – – – – – – 0 – 0
SpindlePole – – – – – – – – – – – – 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – 0
Cytoplasm – – – – – – – – – – – – 0 – 0
Nucleus – – – – – – – – – – – – 0 – 0
Vacuole – – – – – – – – – – – – 4.7 – 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9058 3.3066 3.2839 3.488 3.6679 3.4053 3.4522 3.7683 3.5711 4.1447 4.0389 3.6205 1.321 0.6956 0.8137 0.6612 0.7805 1.2384
Actin 0.0663 0.0019 0.0094 0.0061 0.0256 0.0065 0.0331 0.0002 0.0292 0.0001 0.0377 0.0148 0.0557 0 0.0119 0.0196 0.0303 0.0221
Bud 0.0069 0.0108 0.0052 0.0068 0.0008 0.006 0.0019 0.0001 0.0028 0.0002 0.0043 0.0025 0.003 0.0005 0.011 0.0001 0.0082 0.0005
Bud Neck 0.0073 0.0004 0.0039 0.0048 0.0016 0.0006 0.0085 0.0001 0.0014 0.0001 0.0057 0.0025 0.0099 0 0.0014 0.0001 0.0012 0.0015
Bud Periphery 0.0055 0.0066 0.0045 0.0107 0.0038 0.0055 0.0036 0.0002 0.0073 0.0005 0.0062 0.0074 0.0023 0.0004 0.003 0.0001 0.0154 0.0023
Bud Site 0.0148 0.0104 0.0098 0.0031 0.0009 0.0015 0.01 0.0003 0.0052 0 0.0573 0.0009 0.021 0.0042 0.0099 0.0003 0.0042 0.0011
Cell Periphery 0.0012 0.0004 0.0003 0.0004 0.0001 0.0003 0.0008 0.0003 0.0007 0.0001 0.0007 0.0004 0.0005 0.0002 0.0006 0 0.0004 0.0001
Cytoplasm 0.0386 0.096 0.0442 0.0502 0.0666 0.0961 0.0777 0.2137 0.1142 0.1297 0.1266 0.1825 0.022 0.0895 0.0358 0.0527 0.0479 0.0604
Cytoplasmic Foci 0.0322 0.0053 0.0218 0.0226 0.0132 0.0081 0.0234 0.011 0.0119 0.0017 0.0131 0.0094 0.0318 0.0033 0.0051 0.007 0.008 0.0039
Eisosomes 0.0014 0.0003 0.0003 0.0002 0.0005 0.0002 0.0004 0.0002 0.0004 0.0001 0.0002 0.0002 0.0008 0.0001 0.0003 0.0001 0.0004 0.0002
Endoplasmic Reticulum 0.005 0.0006 0.0008 0.0008 0.0015 0.0008 0.0111 0.0014 0.0052 0.0004 0.0025 0.0018 0.001 0.0004 0.0024 0.0001 0.0021 0.0005
Endosome 0.0248 0.0002 0.0028 0.0058 0.0126 0.0027 0.0442 0.0045 0.0104 0.0002 0.0188 0.0038 0.0119 0.0003 0.0025 0.0004 0.0104 0.0023
Golgi 0.0085 0.0001 0.0018 0.0023 0.0067 0.0011 0.0159 0.0003 0.0029 0 0.0135 0.0009 0.0054 0 0.0011 0.0008 0.0062 0.0017
Lipid Particles 0.0221 0.0002 0.0061 0.0096 0.0291 0.0034 0.0193 0.0003 0.0024 0.0001 0.0046 0.0008 0.0128 0.0001 0.0023 0.0009 0.0052 0.0023
Mitochondria 0.0132 0.0031 0.0027 0.0084 0.0284 0.002 0.0036 0.0251 0.006 0.0001 0.0035 0.001 0.0115 0.0001 0.0036 0.0005 0.0091 0.0079
None 0.6927 0.8535 0.8671 0.8126 0.7702 0.8544 0.7094 0.7401 0.7931 0.8657 0.6933 0.7649 0.76 0.8988 0.891 0.9039 0.8397 0.8836
Nuclear Periphery 0.0157 0.0005 0.0007 0.0019 0.0131 0.0026 0.013 0.0003 0.002 0.0001 0.0028 0.001 0.0062 0.0001 0.0016 0.0001 0.0014 0.0015
Nucleolus 0.0011 0.0007 0.0008 0.0024 0.0007 0.0005 0.0008 0.0001 0.0002 0.0001 0.0005 0.0002 0.0028 0.0002 0.0003 0 0.0004 0.0002
Nucleus 0.0058 0.0036 0.001 0.0022 0.0018 0.0015 0.0011 0.0003 0.0007 0.0003 0.001 0.0007 0.0038 0.0006 0.0008 0.0001 0.0013 0.0011
Peroxisomes 0.0267 0.0006 0.0121 0.0388 0.0113 0.0017 0.0111 0.0001 0.0017 0 0.0018 0.0017 0.0228 0.0001 0.0134 0.0126 0.0041 0.005
Punctate Nuclear 0.0067 0.0042 0.0038 0.0081 0.0103 0.0037 0.003 0.0002 0.0009 0.0001 0.0022 0.0019 0.0125 0.0007 0.0007 0.0003 0.002 0.0011
Vacuole 0.0021 0.0006 0.0007 0.0015 0.0005 0.0005 0.0047 0.0012 0.0011 0.0003 0.003 0.0007 0.0016 0.0004 0.0009 0.0002 0.0016 0.0003
Vacuole Periphery 0.0014 0.0001 0.0002 0.0005 0.0008 0.0003 0.0032 0.0001 0.0003 0 0.0007 0.0002 0.0007 0 0.0003 0 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.2215 44.1983 42.3962 39.5983 39.0878 34.9519 37.059 43.4038 40.5128 43.8526
Translational Efficiency 1.6309 1.8013 1.5671 1.4697 1.9135 2.0768 1.7824 1.4983 1.5432 1.5031

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2152 1546 1955 1544 1901 1266 208 1671 4053 2812 2163 3215

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 694.93 728.80 766.07 786.75 642.74 768.85 875.82 804.02 670.45 746.83 776.62 795.73
Standard Deviation 73.94 95.37 96.72 145.51 73.41 87.68 100.37 118.35 78.16 94.12 102.33 132.37
Intensity Change Log 2 — 0.068655 0.140609 0.179038 — 0.258467 0.446399 0.322996 — 0.162978 0.295635 0.250003

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000089 0.000305 0.000287 0.001316 0.000132 0.000129 0.000330 0.000453 0.000109 0.000226 0.000291 0.000868
Bud Neck 0.015577 0.033054 0.006438 0.009863 0.028272 0.043101 0.002015 0.008284 0.021532 0.037577 0.006012 0.009042
Bud Site 0.003257 0.012241 0.012944 0.048709 0.004556 0.009077 0.002345 0.038918 0.003866 0.010817 0.011925 0.043620
Cell Periphery 0.000133 0.000138 0.000170 0.000309 0.000106 0.001304 0.000184 0.000190 0.000120 0.000663 0.000171 0.000247
Cytoplasm 0.462570* 0.437806* 0.416750* 0.442985* 0.445729* 0.379834* 0.175413* 0.491208* 0.454671* 0.411706* 0.393543* 0.468049*
Cytoplasmic Foci 0.209405* 0.191554* 0.014076 0.024498 0.154883 0.156864 0.013017 0.031969 0.183832* 0.175936 0.013974 0.028381
Eisosomes 0.000173 0.000122 0.000027 0.000065 0.000198 0.000777 0.000094 0.000047 0.000185 0.000417 0.000033 0.000056
Endoplasmic Reticulum 0.000395 0.000826 0.001479 0.002506 0.000886 0.001625 0.005425 0.001397 0.000625 0.001186 0.001859 0.001930
Endosome 0.007497 0.015123 0.001775 0.004760 0.005840 0.016438 0.002785 0.008156 0.006720 0.015715 0.001873 0.006525
Golgi 0.001534 0.002844 0.000143 0.001137 0.002620 0.003556 0.000071 0.002293 0.002043 0.003165 0.000136 0.001738
Lipid Particles 0.008429 0.004671 0.001124 0.001563 0.006498 0.023531 0.002995 0.001265 0.007523 0.013162 0.001304 0.001408
Mitochondria 0.005914 0.007157 0.000850 0.002817 0.004314 0.010205 0.000391 0.004784 0.005163 0.008529 0.000806 0.003840
Mitotic Spindle 0.002349 0.005767 0.006202 0.027209 0.000285 0.003398 0.000291 0.021372 0.001381 0.004701 0.005633 0.024175
None 0.002997 0.004842 0.005331 0.005002 0.003478 0.007514 0.005657 0.004165 0.003223 0.006045 0.005363 0.004567
Nuclear Periphery 0.000456 0.000457 0.001600 0.001699 0.000391 0.001146 0.002172 0.001197 0.000426 0.000767 0.001655 0.001438
Nuclear Periphery Foci 0.000159 0.000374 0.000460 0.001187 0.000144 0.000306 0.001493 0.000548 0.000152 0.000343 0.000559 0.000855
Nucleolus 0.000977 0.002071 0.000489 0.000629 0.000913 0.003113 0.000862 0.000802 0.000947 0.002541 0.000525 0.000719
Nucleus 0.236058* 0.204586* 0.499404* 0.309664* 0.293228* 0.274422* 0.708050* 0.263032* 0.262872* 0.236027* 0.519468* 0.285427*
Peroxisomes 0.006438 0.010212 0.000390 0.002767 0.004292 0.009346 0.000383 0.002252 0.005431 0.009822 0.000389 0.002499
Vacuole 0.034944 0.064203 0.029789 0.108394 0.042682 0.052722 0.075856 0.114402 0.038573 0.059034 0.034219 0.111517
Vacuole Periphery 0.000650 0.001647 0.000272 0.002917 0.000552 0.001591 0.000170 0.003264 0.000604 0.001622 0.000262 0.003097

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.24 -1.81 -5.02 -3.90 -3.53 0.06 -6.99 -5.17 -4.16 1.61 -4.54 -1.98 -6.10 -4.80 -2.92
Bud Neck -7.06 6.53 4.69 10.42 -2.76 -4.16 15.11 11.93 10.95 -6.37 -7.58 12.89 12.11 15.15 -2.84
Bud Site -7.80 -5.34 -10.04 -6.52 -6.15 -3.30 2.28 -8.86 -6.34 -9.41 -7.85 -4.99 -13.41 -9.03 -7.73
Cell Periphery -0.48 -2.45 -4.45 -4.13 -2.74 -7.82 -5.80 -4.28 7.54 2.66 -7.74 -4.27 -5.72 6.74 -1.29
Cytoplasm 3.03 8.98 9.41 5.80 0.89 7.29 20.65 2.01 -4.71 -18.48 6.93 12.38 7.94 1.00 -4.95
Cytoplasmic Foci 2.61 42.69 40.47 32.98 -4.20 -0.46 31.25 29.20 24.26 -5.83 1.52 52.87 49.20 40.53 -7.57
Eisosomes 5.13 19.02 12.69 7.08 -8.26 -7.91 4.42 9.85 10.09 7.76 -6.82 18.02 15.34 10.79 -7.44
Endoplasmic Reticulum -6.74 -11.60 -17.08 -13.58 -5.53 -1.81 -10.40 -6.57 -0.55 8.98 -3.05 -13.06 -17.18 -6.25 0.70
Endosome -5.78 10.47 6.58 9.12 -4.35 -7.00 3.31 0.11 6.85 -2.75 -9.04 12.00 4.22 11.06 -6.85
Golgi -2.88 8.55 4.37 5.12 -3.15 -1.38 8.96 2.96 3.14 -3.64 -2.78 11.87 4.38 5.31 -4.41
Lipid Particles 5.73 11.88 11.63 9.25 -2.33 -9.88 5.55 11.73 13.32 6.68 -6.47 14.70 16.24 14.76 -0.61
Mitochondria -0.93 6.32 4.61 5.50 -3.57 -4.81 7.48 1.51 5.57 -4.10 -3.90 8.72 4.29 7.57 -4.58
Mitotic Spindle -2.20 -3.00 -7.85 -6.09 -6.10 -2.72 -0.93 -9.08 -6.50 -9.02 -3.33 -3.99 -11.60 -8.65 -7.81
None -2.00 -2.86 -2.26 0.74 1.02 -3.19 -0.82 -0.21 3.25 0.76 -3.71 -2.79 -1.63 3.02 1.75
Nuclear Periphery -0.29 -16.68 -17.50 -17.36 -4.53 -4.19 -13.82 -18.14 -4.04 4.56 -4.15 -19.32 -24.75 -13.96 -2.06
Nuclear Periphery Foci -5.04 -5.80 -7.27 -5.55 -4.05 -1.48 -3.05 -7.74 -2.49 2.49 -3.57 -5.98 -9.76 -5.97 -1.01
Nucleolus -3.85 3.09 2.28 6.38 -2.28 -4.31 0.07 0.50 4.33 0.32 -5.75 4.43 1.95 6.71 -2.44
Nucleus 4.36 -33.61 -10.92 -13.61 18.46 2.28 -18.50 -0.25 -2.25 17.94 4.85 -35.58 -8.08 -11.46 26.20
Peroxisomes -3.51 10.28 6.64 8.36 -3.81 -4.89 11.54 5.25 7.27 -3.94 -5.84 14.15 8.44 11.11 -5.33
Vacuole -10.93 -4.06 -23.80 -17.73 -20.90 -2.65 -6.87 -22.77 -19.49 -2.01 -9.47 -5.76 -33.10 -26.20 -25.27
Vacuole Periphery -4.53 5.36 -1.88 0.24 -2.80 -6.06 8.15 -2.97 -0.67 -3.77 -7.15 7.19 -3.45 -0.33 -4.58
Standard name
Human Ortholog
Description Rhodanese domain sulfur transferase; accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Tum1

Tum1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tum1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available