Standard name
Human Ortholog
Description Mitochondrial integral inner membrane protein; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.15 0.06 – 0 – 0 0 0 0 0 0
Bud 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – 0.32 – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.12 – 0.09 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.1 0.06 – 0.05 – 0 0 0 0 0 0
Endosome 0 0 0.12 0.13 0.09 0.07 0 0 0 0 0 0 0.05 0.07 0.08 – 0 – 0.08 0 0.07 0.09 0.07 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.18 0.17 – 0.05 – 0 0 0.05 0 0.05 0.06
Mitochondria 0.91 0.99 0.87 0.9 0.86 0.84 0.87 0.87 0.9 0.94 0.97 0.97 0.66 0.47 0.37 – 0.09 – 0.78 0.77 0.75 0.7 0.69 0.68
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.09 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.1 0 0.06 0.05 0.05 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.08 0.08 0.08 0.07 0.06 0.05 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0.1 0.11 0 0 0 0.05 0 0 0 0 0 0 0 – 0.23 – 0 0 0.11 0.08 0.08 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.05 – 0.05 – 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.11 0.06 0.05 0.11 0.1 0.06 0.05 0 0 0 0.05 0.15 0.13 0.25 – 0.09 – 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 0 0 0 0 0 0 0 0 0 0 26 40 13 – 0 – 0 1 2 1 16 33
Bud 4 10 6 4 4 5 11 24 19 16 27 26 9 6 5 – 1 – 1 1 0 0 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 – 0 – 0 0 0 1 5 8
Bud Site 0 0 1 0 1 5 7 12 9 8 8 10 0 0 0 – 0 – – – – – – –
Cell Periphery 2 1 9 10 2 2 4 5 3 3 5 8 8 10 14 – 7 – 0 0 0 0 0 1
Cytoplasm 3 4 1 5 9 11 4 17 13 10 4 4 6 13 25 – 2 – 0 0 0 0 1 1
Endoplasmic Reticulum 0 5 2 0 1 1 2 1 3 1 2 3 24 27 12 – 1 – 0 0 1 2 6 1
Endosome 6 0 41 38 37 35 26 36 5 7 1 3 13 20 17 – 0 – 13 6 24 22 55 47
Golgi 4 2 2 2 3 1 0 1 0 0 0 1 15 49 36 – 1 – 2 5 16 7 42 49
Mitochondria 138 151 302 262 360 412 537 805 510 639 668 752 163 127 80 – 2 – 132 117 269 176 546 532
Nucleus 1 1 1 0 3 16 13 21 10 9 7 5 2 1 4 – 2 – 0 0 0 1 1 0
Nuclear Periphery 4 1 9 3 8 19 60 32 36 32 38 26 0 3 0 – 0 – 4 0 2 2 3 9
Nucleolus 4 1 3 5 10 38 51 75 42 44 35 23 0 0 0 – 0 – 0 0 0 0 5 2
Peroxisomes 2 0 36 31 17 16 13 46 5 10 7 7 3 7 4 – 5 – 4 3 38 20 63 56
SpindlePole 2 1 15 12 13 18 20 35 20 21 17 25 19 15 10 – 1 – 2 0 1 1 4 4
Vac/Vac Membrane 10 17 22 14 47 48 34 46 21 15 26 38 36 35 54 – 2 – 3 10 0 8 29 23
Unique Cell Count 152 153 347 292 417 492 617 927 565 681 692 775 246 272 216 22 169 152 360 254 792 778
Labelled Cell Count 181 196 450 386 515 627 782 1156 696 815 846 931 324 354 276 24 169 152 360 254 792 778


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.4 5.3 5.4 5.4 4.8 4.5 4.7 4.4 4.1 4.1 4.3 6.2 6.5 6.5 – 5.7 – 6.5 6.7 6.9
Std Deviation (1e-4) 0.7 0.5 1.0 1.0 1.7 1.2 1.1 1.2 1.2 1.1 0.9 0.9 0.7 0.8 0.8 – 1.7 – 1.1 1.5 1.1
Intensity Change (Log2) – – – 0.03 0.04 -0.14 -0.24 -0.17 -0.25 -0.37 -0.36 -0.29 0.23 0.3 0.3 – 0.11 – 0.32 0.35 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3506 2.168 1.7457 1.7592 1.9665 2.2198 1.192 2.091 1.7492 1.381 1.895 1.8297 1.088 1.6189 1.7691 2.0847 1.6761 1.557
Actin 0.0371 0.0064 0.011 0.0033 0.0114 0.0112 0.0287 0.0144 0.0126 0.0003 0.0027 0.0097 0.0037 0.0118 0.0129 0.0003 0.0024 0.0044
Bud 0.0008 0.002 0.0008 0.0027 0.0006 0.0004 0.0004 0.0007 0.0007 0.0001 0.0001 0.0005 0.0003 0.0007 0.0005 0.0002 0.0009 0.0004
Bud Neck 0.0008 0.001 0.0063 0.0003 0.0015 0.001 0.0004 0.0004 0.0004 0.0001 0.0001 0.0004 0.0003 0.0004 0.0011 0.0001 0.0001 0.0007
Bud Periphery 0.0013 0.005 0.0018 0.009 0.0011 0.001 0.0011 0.0012 0.0019 0.0005 0.0002 0.0012 0.0013 0.0007 0.0009 0.0005 0.0044 0.0008
Bud Site 0.0024 0.0184 0.0102 0.0023 0.0021 0.0006 0.0013 0.0021 0.0019 0.0002 0.0002 0.0045 0.001 0.0023 0.0014 0.0008 0.0007 0.0005
Cell Periphery 0.0004 0.001 0.0005 0.0012 0.0002 0.0003 0.0004 0.0003 0.0006 0.0002 0.0001 0.0002 0.0029 0.0002 0.0005 0.0003 0.0008 0.0004
Cytoplasm 0.0064 0.0058 0.0018 0.0086 0.0064 0.0028 0.0008 0.0017 0.0036 0.0006 0.0004 0.0011 0.0006 0.0464 0.0007 0.001 0.0004 0.0025
Cytoplasmic Foci 0.0228 0.0288 0.0279 0.0357 0.03 0.0318 0.0309 0.0258 0.0159 0.0093 0.0151 0.013 0.0173 0.0158 0.0185 0.0013 0.0061 0.0149
Eisosomes 0.0005 0.0003 0.0004 0.0003 0.0003 0.0002 0.0006 0.0003 0.0006 0.0001 0.0001 0.0002 0.0005 0.0003 0.0015 0 0.0004 0.0004
Endoplasmic Reticulum 0.0037 0.0004 0.0005 0.0008 0.0035 0.002 0.001 0.0006 0.0017 0.0002 0.0001 0.0006 0.0015 0.0013 0.0009 0.0019 0.0002 0.0003
Endosome 0.0701 0.0363 0.0404 0.0351 0.0944 0.0837 0.0416 0.0371 0.0382 0.0821 0.0419 0.0362 0.0346 0.0417 0.0476 0.0527 0.0245 0.0296
Golgi 0.0594 0.0507 0.0413 0.0217 0.0468 0.0779 0.055 0.0565 0.0368 0.0191 0.068 0.0458 0.0362 0.104 0.0594 0.0043 0.0648 0.0449
Lipid Particles 0.0139 0.009 0.0108 0.0274 0.0195 0.0147 0.0474 0.0093 0.02 0.004 0.0389 0.0178 0.1211 0.0124 0.0339 0.0033 0.0253 0.0073
Mitochondria 0.7192 0.792 0.8029 0.7751 0.6967 0.7321 0.6923 0.8143 0.7779 0.8424 0.7905 0.8367 0.6697 0.7369 0.7535 0.8793 0.8572 0.8695
None 0.0076 0.002 0.0014 0.0098 0.0038 0.0012 0.0037 0.0005 0.0097 0.0001 0.0001 0.0013 0.0015 0.0004 0.0013 0.0005 0.0002 0.0032
Nuclear Periphery 0.0156 0.0001 0.0009 0.0004 0.0273 0.0005 0.0014 0.0001 0.0076 0.0001 0 0.0002 0.0015 0.0003 0.0006 0.0065 0.0001 0.0001
Nucleolus 0.0004 0.0005 0.001 0.0007 0.0012 0.0002 0.0032 0.0001 0.0008 0.0002 0 0.0001 0.0024 0.0002 0.0014 0.0008 0.0001 0.0001
Nucleus 0.0038 0.0005 0.001 0.0006 0.0031 0.0003 0.0007 0.0001 0.0013 0.0002 0 0.0002 0.0011 0.0002 0.0006 0.0028 0.0001 0.0001
Peroxisomes 0.019 0.0339 0.0282 0.0527 0.0294 0.0284 0.0811 0.0306 0.0498 0.017 0.0392 0.0266 0.0468 0.0192 0.0545 0.0006 0.0067 0.0167
Punctate Nuclear 0.0065 0.0003 0.0009 0.0013 0.0149 0.0004 0.0024 0.0001 0.0007 0 0.0002 0.0001 0.0006 0.0001 0.0004 0.0002 0.0001 0.0001
Vacuole 0.0034 0.0036 0.0067 0.0075 0.0026 0.0047 0.0026 0.0022 0.0111 0.0145 0.0006 0.0016 0.0374 0.0024 0.0047 0.0285 0.0016 0.0016
Vacuole Periphery 0.0048 0.002 0.0033 0.0037 0.0032 0.0046 0.003 0.0016 0.0062 0.0086 0.0015 0.0021 0.0177 0.0023 0.0033 0.014 0.0027 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.0118 5.4282 5.7542 8.8065 9.5599 8.6698 6.8605 7.4086 6.7677 11.7602
Translational Efficiency 0.5561 0.8177 0.9804 0.575 0.6085 0.8997 0.8466 0.8378 0.9684 0.7168

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
94 452 174 1080 1228 637 332 863 1322 1089 506 1943

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 975.05 790.71 1075.04 884.76 623.41 806.38 978.07 858.70 648.41 799.88 1011.42 873.19
Standard Deviation 165.77 123.98 136.12 121.75 88.98 109.38 120.00 109.26 132.19 115.92 133.94 117.08
Intensity Change Log 2 — -0.302328 0.140842 -0.140190 — 0.371279 0.649756 0.461973 — -0.001237 0.361128 0.125271

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.031481 0.003141 0.006571 0.012820 0.000541 0.000763 0.016402 0.007978 0.002741 0.001750 0.013022 0.010669
Bud Neck 0.008986 0.004986 0.006676 0.010813 0.007446 0.004212 0.009756 0.009851 0.007556 0.004533 0.008697 0.010386
Bud Site 0.080772 0.020939 0.036228 0.098357 0.006540 0.005216 0.071765 0.068302 0.011818 0.011742 0.059545 0.085007
Cell Periphery 0.002139 0.000666 0.000350 0.000455 0.002372 0.001189 0.000408 0.001149 0.002355 0.000972 0.000388 0.000763
Cytoplasm 0.023823 0.002997 0.003920 0.035138 0.003292 0.001327 0.011104 0.017151 0.004752 0.002020 0.008633 0.027149
Cytoplasmic Foci 0.124707 0.038715 0.137702 0.103308 0.017214 0.039340 0.096076 0.074087 0.024857 0.039081 0.110390 0.090329
Eisosomes 0.000383 0.000095 0.000095 0.000144 0.000072 0.000071 0.000150 0.000153 0.000094 0.000081 0.000131 0.000148
Endoplasmic Reticulum 0.005314 0.000458 0.001422 0.001984 0.000448 0.000237 0.003152 0.001135 0.000794 0.000328 0.002557 0.001607
Endosome 0.123868 0.098726 0.149295 0.092181 0.031709 0.091269 0.097000 0.090797 0.038262 0.094364 0.114983 0.091566
Golgi 0.129546* 0.091790 0.195751* 0.119010* 0.044835 0.099586 0.114528* 0.134035* 0.050858 0.096350 0.142459* 0.125684*
Lipid Particles 0.003129 0.004072 0.001212 0.001024 0.006266 0.008000 0.001216 0.000853 0.006043 0.006370 0.001215 0.000948
Mitochondria 0.273230* 0.650747* 0.183614* 0.271854* 0.837338* 0.676988* 0.273015* 0.445408* 0.797228* 0.666096* 0.242272* 0.348939*
Mitotic Spindle 0.018147 0.007731 0.120733* 0.075253 0.002567 0.004814 0.138525* 0.029847 0.003675 0.006024 0.132407* 0.055086
None 0.000141 0.000285 0.000215 0.000349 0.000582 0.000206 0.000213 0.000220 0.000550 0.000239 0.000213 0.000292
Nuclear Periphery 0.000500 0.000100 0.002191 0.000238 0.000123 0.000072 0.000205 0.000257 0.000150 0.000084 0.000888 0.000246
Nuclear Periphery Foci 0.000792 0.000146 0.001828 0.000529 0.000198 0.000163 0.000601 0.001089 0.000241 0.000156 0.001023 0.000777
Nucleolus 0.000131 0.000512 0.000848 0.000407 0.000980 0.000527 0.000409 0.000326 0.000919 0.000521 0.000560 0.000371
Nucleus 0.001770 0.001950 0.001850 0.002414 0.000892 0.000566 0.001452 0.001435 0.000955 0.001140 0.001589 0.001979
Peroxisomes 0.066965 0.047778 0.050245 0.075265 0.024094 0.038483 0.112647* 0.056411 0.027143 0.042341 0.091189 0.066891
Vacuole 0.064269 0.010459 0.058454 0.075214 0.003403 0.011305 0.033712 0.040929 0.007731 0.010954 0.042220 0.059986
Vacuole Periphery 0.039905 0.013706 0.040799 0.023244 0.009088 0.015667 0.017666 0.018589 0.011279 0.014853 0.025621 0.021176

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.78 2.41 1.82 -5.01 -2.54 -0.69 -5.15 -5.06 -4.81 2.46 1.16 -4.59 -5.72 -7.26 1.01
Bud Neck 1.75 0.96 -1.06 -3.76 -2.56 1.65 -1.10 -1.25 -3.25 0.01 1.79 -0.69 -1.89 -5.09 -1.24
Bud Site 4.15 3.02 -1.31 -12.38 -9.19 0.79 -9.37 -12.46 -12.37 0.44 0.02 -9.43 -19.83 -18.64 -4.28
Cell Periphery 2.36 2.90 2.74 1.82 -1.09 1.09 3.99 1.97 0.04 -1.92 1.90 4.29 3.26 0.36 -2.09
Cytoplasm 2.17 2.08 -1.34 -12.00 -12.00 2.47 -2.70 -5.45 -9.05 -1.97 2.77 -1.37 -9.60 -14.94 -8.62
Cytoplasmic Foci 5.61 -0.71 1.44 -12.68 3.51 -6.95 -12.78 -14.46 -7.60 3.10 -4.87 -15.66 -20.17 -14.98 3.49
Eisosomes 4.18 4.18 3.47 -3.96 -3.95 0.07 -6.67 -8.07 -7.37 -0.16 1.47 -3.58 -5.71 -8.10 -1.64
Endoplasmic Reticulum 1.79 1.43 1.14 -4.43 -1.71 3.09 -3.33 -2.71 -3.61 2.44 2.24 -3.07 -2.95 -5.75 1.52
Endosome 1.31 -1.29 1.82 0.94 5.43 -9.42 -9.36 -12.65 0.08 0.69 -10.62 -12.36 -13.58 0.66 3.77
Golgi 2.11 -3.07 0.76 -2.94 5.25 -7.84 -7.78 -13.11 -3.93 -2.12 -8.20 -11.12 -14.51 -4.60 2.04
Lipid Particles -0.39 1.95 2.11 2.11 0.20 -0.81 3.86 4.33 4.00 2.04 -0.19 4.05 4.38 4.49 1.68
Mitochondria -9.89 2.24 0.19 20.23 -3.83 10.31 29.99 27.77 13.05 -8.40 9.64 35.41 39.31* 24.83 -6.91
Mitotic Spindle 1.86 -6.81 -8.11 -13.36 3.01 -1.93 -10.81 -8.96 -7.94 8.46 -2.05 -13.41 -16.74 -15.63 7.74
None -2.67 -2.50 -1.76 -0.69 -1.23 2.97 3.11 3.02 -0.23 -0.19 2.74 3.07 1.83 -0.86 -1.25
Nuclear Periphery 1.72 -0.89 0.95 -2.28 1.02 0.65 -1.18 -0.80 -1.23 -0.21 0.86 -1.16 -1.18 -2.44 0.97
Nuclear Periphery Foci 2.19 -0.79 0.74 -3.39 1.05 0.66 -2.57 -0.99 -1.05 -0.39 1.36 -2.02 -1.38 -1.69 0.50
Nucleolus -0.98 -1.45 -2.43 0.10 0.79 0.76 0.86 1.11 0.67 0.53 0.73 0.56 0.96 0.46 0.83
Nucleus 0.13 0.19 -0.46 -0.57 -0.83 0.96 -1.53 -0.98 -1.60 0.46 -0.20 -1.51 -2.64 -1.48 -0.59
Peroxisomes 1.47 1.19 -0.59 -3.39 -2.56 -2.70 -8.04 -6.20 -2.72 4.80 -3.40 -7.94 -9.42 -4.75 2.93
Vacuole 3.76 0.45 -1.07 -15.25 -2.66 -5.76 -7.72 -10.99 -8.30 -0.76 -1.95 -8.88 -17.62 -17.16 -3.97
Vacuole Periphery 2.38 -0.09 1.55 -3.54 3.13 -3.66 -2.40 -5.47 -1.33 -0.33 -2.19 -4.51 -5.82 -3.57 1.43
Standard name
Human Ortholog
Description Mitochondrial integral inner membrane protein; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine
Localization
Cell Percentages mitochondrion (92%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Pnt1

Pnt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pnt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available