Standard name
Description Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; also has a role in tRNA gene-mediated silencing; gene encodes two isozymic forms; converts to a prion form, prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis; homolog of human TRIT1, a mutation in which is associated with severe combined respiratory chain defects
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.15 0.14 0.22 0.18 0.16 0.11 0.11 0.08 0.07 0.05 0 0.41 0.39 0.46 0.31 0.27 0.25 0 0.12 0.05 0.1 0.14 0.19
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.1 0 0.18 0.13 0.32 0.5 0.33 0.38 0.54 0.54 0.59 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.56 0.44 0.54 0.34 0.39 0.43 0.42 0.44 0.48 0.5 0.54 0.47 0.43 0.31 0.22 0.41 0.37 0.36 0.68 0.58 0.57 0.53 0.4 0.28
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.32 0.48 0.41 0.4 0.45 0.3 0.22 0.24 0.23 0.17 0.17 0.19 0.41 0.5 0.48 0.31 0.41 0.44 0.23 0.2 0.26 0.3 0.33 0.35
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 1 0 1 1
Bud 0 0 0 1 0 3 13 9 5 14 6 7 0 0 0 0 0 0 3 6 7 4 7 12
Bud Neck 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5
Bud Site 0 0 0 0 0 2 7 4 5 18 9 11 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 2 0 0 0 1 0 0 0 1 1 1 1 0 0 0 0 0 0 0
Cytoplasm 19 30 56 55 59 77 75 79 46 47 21 16 122 135 176 86 53 56 8 28 23 33 55 90
Endoplasmic Reticulum 0 2 2 1 0 1 0 0 1 0 0 1 1 3 1 16 11 13 0 0 4 0 3 5
Endosome 2 0 5 2 10 3 1 7 8 0 0 0 0 6 12 4 4 8 5 4 5 3 10 19
Golgi 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 1 2 2 0 0 2 1 0 3
Mitochondria 51 20 10 43 42 158 352 228 217 377 243 314 0 0 0 3 8 3 5 4 11 6 14 12
Nucleus 177 88 212 84 130 208 299 303 270 345 240 251 129 107 85 112 72 80 262 133 244 179 158 130
Nuclear Periphery 3 0 0 0 0 4 2 2 6 5 4 3 0 0 0 1 1 0 1 2 4 0 1 4
Nucleolus 8 1 0 1 0 0 5 12 3 15 10 12 0 0 0 2 0 0 1 0 1 0 2 2
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 2 2 5 4 5 6
Vac/Vac Membrane 103 97 159 97 152 147 156 164 133 119 74 102 121 172 187 86 80 98 89 46 110 101 131 165
Unique Cell Count 317 202 390 245 335 488 707 689 566 693 448 534 298 345 386 274 197 221 387 232 429 339 400 470
Labelled Cell Count 365 239 445 284 394 605 910 808 694 943 607 717 374 424 462 312 232 260 387 232 429 339 400 470


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 6.5 6.7 6.2 6.1 5.3 5.1 5.4 5.4 5.0 5.0 4.9 7.9 7.9 7.7 8.0 7.5 7.8 8.4 8.6 8.7
Std Deviation (1e-4) 0.8 1.2 1.1 1.7 1.6 1.1 1.1 1.3 1.2 1.2 1.2 1.2 1.0 1.3 1.2 1.7 1.6 1.8 1.3 1.3 1.3
Intensity Change (Log2) – – – -0.12 -0.14 -0.35 -0.4 -0.32 -0.31 -0.43 -0.41 -0.46 0.23 0.25 0.2 0.26 0.17 0.22 0.33 0.36 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2.8 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 3.2 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.6 1.2 0.6 -1.8 -1.4 -3.1 -4.1 -4.8 -6.4 7.9 7.6 9.5 5.3 3.7 3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 4.2 0 4.1
Endosome 0 1.6 0 0 0 0.2 0 0 0 0 0.5 1.7 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.6 5.2 11.2 15.9 11.6 12.8 17.1 16.3 0 0 0 0 -1.3 1.0 0
Nucleus -4.9 -4.2 -3.5 -3.8 -3.3 -2.0 -1.4 -0.2 -2.2 -2.9 -6.4 -9.3 -3.4 -4.1 -4.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 2.9 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.3 1.2 -3.3 -6.6 -5.8 -5.7 -8.5 -7.8 -7.2 0 2.5 2.2 -2.5 0 0.9