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Description | Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; also has a role in tRNA gene-mediated silencing; gene encodes two isozymic forms; converts to a prion form, prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis; homolog of human TRIT1, a mutation in which is associated with severe combined respiratory chain defects |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 1 | 0 | 3 | 13 | 9 | 5 | 14 | 6 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 7 | 4 | 7 | 12 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 7 | 4 | 5 | 18 | 9 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 19 | 30 | 56 | 55 | 59 | 77 | 75 | 79 | 46 | 47 | 21 | 16 | 122 | 135 | 176 | 86 | 53 | 56 | 8 | 28 | 23 | 33 | 55 | 90 |
Endoplasmic Reticulum | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 1 | 16 | 11 | 13 | 0 | 0 | 4 | 0 | 3 | 5 |
Endosome | 2 | 0 | 5 | 2 | 10 | 3 | 1 | 7 | 8 | 0 | 0 | 0 | 0 | 6 | 12 | 4 | 4 | 8 | 5 | 4 | 5 | 3 | 10 | 19 |
Golgi | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 2 | 1 | 0 | 3 |
Mitochondria | 51 | 20 | 10 | 43 | 42 | 158 | 352 | 228 | 217 | 377 | 243 | 314 | 0 | 0 | 0 | 3 | 8 | 3 | 5 | 4 | 11 | 6 | 14 | 12 |
Nucleus | 177 | 88 | 212 | 84 | 130 | 208 | 299 | 303 | 270 | 345 | 240 | 251 | 129 | 107 | 85 | 112 | 72 | 80 | 262 | 133 | 244 | 179 | 158 | 130 |
Nuclear Periphery | 3 | 0 | 0 | 0 | 0 | 4 | 2 | 2 | 6 | 5 | 4 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 4 | 0 | 1 | 4 |
Nucleolus | 8 | 1 | 0 | 1 | 0 | 0 | 5 | 12 | 3 | 15 | 10 | 12 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 2 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 5 | 4 | 5 | 6 |
Vac/Vac Membrane | 103 | 97 | 159 | 97 | 152 | 147 | 156 | 164 | 133 | 119 | 74 | 102 | 121 | 172 | 187 | 86 | 80 | 98 | 89 | 46 | 110 | 101 | 131 | 165 |
Unique Cell Count | 317 | 202 | 390 | 245 | 335 | 488 | 707 | 689 | 566 | 693 | 448 | 534 | 298 | 345 | 386 | 274 | 197 | 221 | 387 | 232 | 429 | 339 | 400 | 470 |
Labelled Cell Count | 365 | 239 | 445 | 284 | 394 | 605 | 910 | 808 | 694 | 943 | 607 | 717 | 374 | 424 | 462 | 312 | 232 | 260 | 387 | 232 | 429 | 339 | 400 | 470 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 6.5 | 6.7 | 6.2 | 6.1 | 5.3 | 5.1 | 5.4 | 5.4 | 5.0 | 5.0 | 4.9 | 7.9 | 7.9 | 7.7 | 8.0 | 7.5 | 7.8 | 8.4 | 8.6 | 8.7 |
Std Deviation (1e-4) | 0.8 | 1.2 | 1.1 | 1.7 | 1.6 | 1.1 | 1.1 | 1.3 | 1.2 | 1.2 | 1.2 | 1.2 | 1.0 | 1.3 | 1.2 | 1.7 | 1.6 | 1.8 | 1.3 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.14 | -0.35 | -0.4 | -0.32 | -0.31 | -0.43 | -0.41 | -0.46 | 0.23 | 0.25 | 0.2 | 0.26 | 0.17 | 0.22 | 0.33 | 0.36 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.6 | 1.2 | 0.6 | -1.8 | -1.4 | -3.1 | -4.1 | -4.8 | -6.4 | 7.9 | 7.6 | 9.5 | 5.3 | 3.7 | 3.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 0 | 4.1 |
Endosome | 0 | 1.6 | 0 | 0 | 0 | 0.2 | 0 | 0 | 0 | 0 | 0.5 | 1.7 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6.6 | 5.2 | 11.2 | 15.9 | 11.6 | 12.8 | 17.1 | 16.3 | 0 | 0 | 0 | 0 | -1.3 | 1.0 | 0 |
Nucleus | -4.9 | -4.2 | -3.5 | -3.8 | -3.3 | -2.0 | -1.4 | -0.2 | -2.2 | -2.9 | -6.4 | -9.3 | -3.4 | -4.1 | -4.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.3 | 1.2 | -3.3 | -6.6 | -5.8 | -5.7 | -8.5 | -7.8 | -7.2 | 0 | 2.5 | 2.2 | -2.5 | 0 | 0.9 |