Standard name
Human Ortholog
Description Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.99 1.0 0.97 0.98 0.98 0.93 0.95 0.88 0.89 0.84 0.83 1.0 1.0 1.0 0.6 0.57 0.74 0.65 0.67 0.68 0.79 0.76 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0 0 0 0.08 0.05 0.17 0.11 0.21 0.09 0 0 0 0 0.05 0 0.15 0.07 0.12 0.07 0.07 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.05 0.08 0.1 0 0 0 0.47 0.51 0.32 0.1 0.2 0.1 0.06 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3 1 1 3 9 6
Bud 2 0 0 0 0 1 9 5 9 8 7 4 0 0 0 0 0 0 1 0 1 1 10 11
Bud Neck 0 0 3 1 5 9 11 12 2 4 1 2 2 2 1 0 0 0 3 1 1 2 1 4
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 2 1 0 2 0 1 1 0 1 0 4 1 1 0 0 1 1 0 0 1 6 7
Cytoplasm 212 172 146 146 307 277 356 367 247 298 244 235 369 537 557 150 93 148 158 117 97 191 328 298
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 10 2 8 2 0 0 0 2 3
Endosome 0 0 0 1 0 0 0 1 0 2 0 0 0 0 0 9 9 6 2 1 2 0 2 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2
Mitochondria 23 3 2 4 1 9 30 19 48 37 60 25 0 0 0 4 8 6 37 13 16 16 31 32
Nucleus 0 1 0 1 3 2 3 8 4 5 5 9 1 0 2 20 14 9 0 0 0 1 0 0
Nuclear Periphery 0 0 0 1 1 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 1 0 0 1 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
SpindlePole 2 0 1 0 3 3 4 2 1 2 0 1 0 0 1 0 2 0 2 0 0 3 8 8
Vac/Vac Membrane 1 2 0 0 0 3 5 5 10 16 23 29 1 0 0 118 83 65 23 35 14 13 18 24
Unique Cell Count 223 173 146 151 312 283 381 386 282 333 289 283 370 537 559 251 163 201 244 176 143 243 433 417
Labelled Cell Count 243 178 154 156 320 307 419 421 324 373 342 308 377 540 562 312 211 244 244 176 143 243 433 417


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 4.1 4.0 4.3 4.7 4.0 3.7 4.2 3.6 3.7 3.5 4.2 4.6 4.9 4.9 6.6 6.7 6.3 4.3 4.9 5.4
Std Deviation (1e-4) 0.4 0.9 0.9 1.9 1.7 1.6 1.3 1.5 1.5 1.1 1.0 1.2 0.9 1.3 1.2 1.7 1.8 1.7 1.1 1.6 1.9
Intensity Change (Log2) – – – 0.11 0.24 -0.01 -0.11 0.07 -0.14 -0.1 -0.2 0.07 0.2 0.29 0.3 0.74 0.74 0.66 0.12 0.29 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 9.9 10.1 7.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.2604 -0.6704 -0.9287 -0.9608 -0.9975 -0.7999 -0.8054 -0.6657 -0.6924 -0.9621 -0.8077 -0.7144 -1.3947 -1.1561 -1.2463 -0.7297 -0.8577 -1.3267
Actin 0.0037 0.0003 0.0001 0.0001 0.0003 0.0001 0.0225 0.0001 0.0095 0.0003 0.0027 0.0013 0.0181 0.0021 0.0066 0.0423 0.0296 0.0004
Bud 0.0065 0.0094 0.0044 0 0.0185 0.0017 0.0048 0.0002 0.0015 0.0002 0.0009 0.0029 0.0018 0.0068 0.0055 0.0074 0.0379 0.0016
Bud Neck 0.0012 0.0001 0.0001 0 0.0001 0.0003 0.0025 0.0004 0.0012 0.0005 0.0004 0.0023 0.0114 0.0004 0.0009 0.0014 0.0008 0.0007
Bud Periphery 0.0039 0.0022 0.0005 0 0.0046 0.0016 0.0105 0.0004 0.0029 0.0003 0.0015 0.0026 0.0042 0.0041 0.0023 0.0137 0.0158 0.0015
Bud Site 0.0051 0.0106 0.0019 0 0.0052 0.0001 0.0025 0.0011 0.0046 0.0055 0.0004 0.0005 0.0054 0.0105 0.0083 0.0067 0.0106 0.0004
Cell Periphery 0.0005 0.0004 0.0001 0 0.0004 0 0.0005 0.0002 0.0003 0.0001 0.0002 0.0004 0.0005 0.0004 0.0003 0.0006 0.002 0.0002
Cytoplasm 0.0164 0.0364 0.0438 0.0109 0.0311 0.0299 0.0332 0.058 0.0376 0.026 0.0489 0.0605 0.0699 0.1405 0.1031 0.0342 0.109 0.1082
Cytoplasmic Foci 0.0065 0.0043 0.006 0.0034 0.0056 0.0114 0.0131 0.0148 0.0179 0.0338 0.0237 0.0113 0.0233 0.0272 0.0178 0.0446 0.0517 0.0297
Eisosomes 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0003 0.0001 0.0001 0.0001 0.0004 0.0001 0.0003 0.0005 0.0002 0.0001
Endoplasmic Reticulum 0.0009 0.0005 0.001 0.0002 0.0002 0.0003 0.0012 0.0005 0.0012 0.0005 0.0016 0.0007 0.0029 0.0017 0.0021 0.0032 0.0032 0.0009
Endosome 0.004 0.0042 0.0048 0.0002 0.0002 0.002 0.0097 0.0038 0.0206 0.0135 0.0345 0.0083 0.0182 0.0354 0.0187 0.0576 0.1097 0.0225
Golgi 0.0011 0.0001 0.0001 0 0 0.0001 0.0036 0.0002 0.0014 0.0005 0.002 0.0007 0.005 0.0008 0.0044 0.006 0.0141 0.0003
Lipid Particles 0.0014 0.0007 0.0004 0.0002 0.0002 0.0003 0.0079 0.0003 0.0024 0.001 0.0234 0.0012 0.0085 0.0017 0.0042 0.0163 0.0776 0.0022
Mitochondria 0.0009 0.0005 0.0007 0.0003 0.0013 0.0003 0.0029 0.0006 0.0077 0.0006 0.0021 0.0016 0.0052 0.001 0.0063 0.0221 0.007 0.001
None 0.9357 0.9122 0.927 0.9832 0.9273 0.9475 0.85 0.9121 0.8722 0.9083 0.8128 0.8718 0.7874 0.7078 0.7805 0.6227 0.3922 0.7923
Nuclear Periphery 0.0022 0.0009 0.0004 0.0001 0.0002 0.0003 0.0043 0.0002 0.0028 0.0004 0.0049 0.0016 0.0058 0.0013 0.0056 0.0203 0.0087 0.0034
Nucleolus 0.001 0.0004 0.0006 0.0002 0.0008 0.0003 0.0027 0.0009 0.0007 0.0006 0.0015 0.0009 0.0012 0.0016 0.0013 0.0042 0.0013 0.0013
Nucleus 0.0022 0.0034 0.0028 0.0002 0.0014 0.0016 0.0046 0.0018 0.0017 0.0012 0.0058 0.0034 0.007 0.0041 0.0038 0.0106 0.0032 0.0026
Peroxisomes 0.0004 0.0002 0.0002 0.0002 0.0002 0.0004 0.0122 0.0002 0.0029 0.001 0.0044 0.0005 0.0059 0.001 0.0009 0.0045 0.0119 0.0007
Punctate Nuclear 0.0031 0.0007 0.0008 0.0002 0.0016 0.001 0.0078 0.0008 0.0026 0.001 0.0071 0.0082 0.0019 0.006 0.0035 0.034 0.0075 0.01
Vacuole 0.0025 0.0115 0.0038 0.0002 0.0006 0.0006 0.0026 0.0032 0.0064 0.0042 0.0191 0.0188 0.0148 0.0436 0.0206 0.0373 0.096 0.0183
Vacuole Periphery 0.0003 0.0009 0.0004 0 0.0001 0.0001 0.0006 0.0002 0.0016 0.0005 0.0021 0.0006 0.0014 0.0019 0.0028 0.0096 0.0099 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.3302 18.5525 7.6482 19.5258 32.1402 19.5899 14.5899 14.7708 16.1548 26.6648
Translational Efficiency 1.9715 0.9266 1.6913 1.0237 0.8817 2.2633 1.1889 1.029 1.2179 1.2614

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1420 493 1815 1167 268 1912 222 1252 1688 2405 2037 2419

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 670.58 733.71 820.95 779.01 679.82 712.94 875.75 858.05 672.05 717.20 826.92 819.92
Standard Deviation 69.85 90.44 96.93 109.58 71.54 84.77 98.22 128.99 70.20 86.37 98.56 126.35
Intensity Change Log 2 — 0.129800 0.291885 0.216232 — 0.068628 0.365366 0.335909 — 0.099329 0.329345 0.277720

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000114 0.000457 0.000145 0.000700 0.000054 0.000177 0.000253 0.001791 0.000104 0.000234 0.000156 0.001265
Bud Neck 0.023924 0.045949 0.003596 0.005413 0.008284 0.046404 0.001604 0.006170 0.021441 0.046311 0.003379 0.005805
Bud Site 0.003286 0.017160 0.004529 0.024627 0.001468 0.012543 0.003312 0.042138 0.002997 0.013489 0.004396 0.033690
Cell Periphery 0.000139 0.000126 0.000128 0.000363 0.000183 0.000158 0.000350 0.000237 0.000146 0.000151 0.000152 0.000298
Cytoplasm 0.426105* 0.310251* 0.310705* 0.475835* 0.436642* 0.393566* 0.264088* 0.469970* 0.427778* 0.376487* 0.305624* 0.472799*
Cytoplasmic Foci 0.288646* 0.306661* 0.006510 0.040922 0.270020* 0.252276* 0.053005 0.036019 0.285689* 0.263425* 0.011577 0.038384
Eisosomes 0.000256 0.000162 0.000027 0.000099 0.000191 0.000157 0.000193 0.000075 0.000246 0.000158 0.000045 0.000087
Endoplasmic Reticulum 0.000602 0.000846 0.002907 0.004484 0.000449 0.000574 0.001874 0.002425 0.000578 0.000630 0.002795 0.003419
Endosome 0.014715 0.035411 0.001237 0.004771 0.011267 0.023989 0.003931 0.009385 0.014167 0.026331 0.001531 0.007159
Golgi 0.002646 0.007907 0.000047 0.002378 0.001104 0.004654 0.000713 0.007984 0.002401 0.005321 0.000120 0.005280
Lipid Particles 0.013608 0.007305 0.000933 0.002591 0.009728 0.008463 0.003881 0.002667 0.012992 0.008226 0.001254 0.002630
Mitochondria 0.006960 0.014930 0.000441 0.001003 0.000855 0.013897 0.004412 0.007733 0.005991 0.014109 0.000874 0.004486
Mitotic Spindle 0.001213 0.006391 0.002272 0.008563 0.000035 0.006951 0.000586 0.025991 0.001026 0.006837 0.002088 0.017583
None 0.002744 0.001354 0.004260 0.006273 0.001543 0.002553 0.012367 0.002821 0.002554 0.002307 0.005144 0.004486
Nuclear Periphery 0.000453 0.000524 0.002143 0.001026 0.000448 0.000412 0.001024 0.001303 0.000452 0.000435 0.002021 0.001169
Nuclear Periphery Foci 0.000180 0.000713 0.000917 0.003798 0.000365 0.000263 0.001116 0.002697 0.000210 0.000355 0.000938 0.003228
Nucleolus 0.000992 0.002735 0.000414 0.000355 0.000655 0.002355 0.000977 0.000311 0.000939 0.002433 0.000476 0.000332
Nucleus 0.160075* 0.104373 0.626353* 0.215627* 0.206871* 0.144321* 0.563854* 0.183343* 0.167505* 0.136132* 0.619542* 0.198918*
Peroxisomes 0.011052 0.034059 0.000124 0.001387 0.004999 0.014649 0.001231 0.002028 0.010091 0.018628 0.000244 0.001719
Vacuole 0.041277 0.100532 0.032214 0.198881 0.044387 0.069209 0.080555 0.190106 0.041771 0.075630 0.037482 0.194339
Vacuole Periphery 0.001013 0.002153 0.000097 0.000905 0.000452 0.002429 0.000674 0.004806 0.000924 0.002372 0.000160 0.002924

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.30 -4.46 -7.68 -2.11 -6.44 -7.39 -2.82 -3.79 -3.27 -2.00 -6.13 -5.45 -5.48 -4.46 -4.52
Bud Neck -4.42 10.59 9.74 9.00 -1.51 -13.34 4.01 1.80 16.33 -5.86 -9.28 10.95 10.05 18.62 -2.82
Bud Site -6.90 -1.48 -6.17 -0.45 -5.24 -9.70 -1.69 -8.01 -3.52 -5.63 -10.07 -1.92 -9.63 -3.46 -7.95
Cell Periphery 1.22 0.66 -5.91 -6.27 -6.08 1.69 -4.47 1.40 -0.20 5.34 -0.54 -1.00 -5.23 -4.81 -4.21
Cytoplasm 8.65 16.03 2.63 -6.33 -11.82 2.64 9.34 3.37 1.40 -8.35 6.18 18.14 4.67 -1.58 -14.20
Cytoplasmic Foci -1.19 47.04 41.38 26.81 -14.57 1.24 16.25 18.45 42.54 4.66 3.23 50.09 46.23 49.70 -14.23
Eisosomes 2.33 5.55 4.00 6.69 -13.50 2.40 -0.32 8.16 13.06 8.41 2.60 5.74 4.77 13.83 -8.95
Endoplasmic Reticulum -1.50 -14.59 -14.37 -14.23 -1.99 -1.07 -6.71 -12.55 -12.85 1.56 -0.44 -16.05 -16.54 -19.69 1.29
Endosome -5.93 11.38 9.13 9.78 -5.06 -4.63 2.60 2.47 14.02 -0.35 -7.71 11.68 8.86 17.94 -6.52
Golgi -2.01 9.55 1.82 2.51 -1.48 -5.76 1.20 -1.83 1.64 -2.44 -4.16 9.64 0.51 3.60 -3.26
Lipid Particles 5.01 12.18 10.84 6.32 -9.28 0.79 3.36 4.82 11.55 4.50 4.81 12.82 11.79 13.34 -5.75
Mitochondria -2.34 5.77 5.50 4.65 -1.66 -10.27 -0.87 -2.13 6.77 0.02 -5.36 5.28 3.73 9.48 -1.95
Mitotic Spindle -1.96 -1.15 -3.07 -0.09 -2.43 -5.40 -1.49 -6.99 -4.00 -6.72 -4.56 -1.33 -7.03 -3.12 -6.32
None 1.99 -1.30 -1.30 -3.14 -0.53 -2.50 -1.80 -3.50 0.31 1.56 0.27 -2.23 -1.08 -1.80 1.39
Nuclear Periphery -0.88 -24.52 -11.69 -8.00 8.23 0.43 -8.04 -9.82 -10.63 -3.61 0.17 -25.62 -14.33 -14.55 4.79
Nuclear Periphery Foci -2.53 -7.83 -5.88 -4.60 -4.10 1.33 -4.11 -8.48 -9.01 -0.25 -2.91 -8.71 -8.84 -8.18 -4.97
Nucleolus -2.86 7.16 9.29 3.93 1.67 -5.37 -1.67 4.03 6.63 5.22 -5.37 6.66 10.06 7.71 2.97
Nucleus 6.45 -60.75 -10.12 -13.96 42.54 4.78 -14.06 -0.56 -9.41 15.61 5.62 -61.04 -9.58 -15.71 51.62
Peroxisomes -6.35 11.80 10.32 9.56 -2.13 -6.75 3.21 3.48 13.79 0.27 -6.45 12.29 10.98 16.66 -2.85
Vacuole -9.58 -3.19 -29.81 -16.29 -27.86 -3.74 -6.62 -25.67 -27.53 -9.76 -12.13 -5.97 -42.30 -34.75 -36.60
Vacuole Periphery -3.28 4.16 1.71 4.93 -1.81 -6.71 -0.18 -2.26 0.87 -1.87 -4.90 4.11 -0.70 2.90 -3.12
Standard name
Human Ortholog
Description Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Mpd1

Mpd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mpd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available