ORF
Human Ortholog
Description Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.94 0.98 0.91 0.75 0.52 0.32 0.35 0.34 0.3 0.18 0.19 0.99 0.99 0.99 0.95 0.91 0.95 0.87 0.86 0.86 0.91 0.84 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.15 0.07 0.12 0.06 0.34 0.29 0.37 0.37 0 0 0 0 0 0 0 0 0.07 0 0 0
Nucleus 0 0 0 0 0.08 0.19 0.27 0.23 0.18 0.24 0.28 0.3 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.06 0 0 0.1 0.32 0.45 0.46 0.32 0.3 0.34 0.34 0 0 0 0.07 0.14 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 1 2
Bud 0 3 0 0 0 0 0 0 2 1 1 2 0 0 0 0 0 0 1 1 0 0 4 5
Bud Neck 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 0 0 0 1 0 0 0 0 3 0 1 1 0 3 3 2 0 0 0 0 0 0
Cytoplasm 204 428 53 48 44 102 61 84 67 43 46 39 185 251 261 285 279 233 186 423 79 193 197 247
Endoplasmic Reticulum 1 2 0 2 1 2 0 0 0 0 0 1 1 1 1 11 12 9 0 0 0 2 2 2
Endosome 0 0 0 0 0 4 4 3 2 1 6 2 0 2 1 1 1 1 3 3 0 3 4 2
Golgi 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 6 0 2 0 0 0 1 0
Mitochondria 3 15 0 0 9 14 23 14 68 42 95 77 0 0 0 7 4 5 1 12 6 2 3 7
Nucleus 1 5 0 1 5 38 52 55 36 34 72 62 0 4 1 4 7 3 7 15 0 1 3 4
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 0 0 1 0 0 2 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 2 0 3 4 0 1 1 6
Vac/Vac Membrane 10 29 0 2 6 63 85 110 64 43 89 71 1 0 1 20 43 27 3 21 1 1 6 4
Unique Cell Count 213 455 54 53 59 195 190 238 198 144 258 210 186 253 263 301 307 245 214 493 92 212 235 296
Labelled Cell Count 223 486 54 53 65 227 225 266 241 165 315 256 188 259 266 331 358 280 214 493 92 212 235 296


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.4 5.2 5.4 5.0 7.0 6.8 7.6 6.2 6.4 6.6 6.6 6.3 6.4 6.5 6.7 7.7 8.1 6.4 7.2 7.1
Std Deviation (1e-4) 1.0 0.9 0.5 1.0 1.1 1.4 1.7 1.8 1.8 1.8 1.8 1.8 1.2 1.2 1.4 1.9 2.1 2.3 2.0 2.1 2.0
Intensity Change (Log2) – – – 0.06 -0.04 0.44 0.4 0.56 0.26 0.32 0.36 0.35 0.27 0.31 0.32 0.38 0.58 0.65 0.31 0.48 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 2.9 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4866 0.7708 0.7117 0.2358 0.2676 0.7834 6.0532 6.2699 5.7289 6.8525 4.5991 5.7988 2.8455 3.2004 2.8976 2.8295 2.5898 2.6909
Actin 0.0003 0.0006 0.0011 0.0007 0.0002 0.0023 0.0525 0.0013 0.0198 0.0053 0.0018 0.0126 0.0429 0.0003 0.0039 0.0003 0.0304 0.0018
Bud 0.0003 0.0017 0.0019 0.0004 0.0001 0.0001 0.0009 0.0057 0.0007 0.0009 0.0004 0.0021 0.001 0.0017 0.0003 0.0001 0.0025 0.0002
Bud Neck 0.0002 0.0005 0.0004 0.0006 0.0006 0.0013 0.0014 0.0007 0.0089 0.0336 0.0009 0.0035 0.0055 0.0003 0.0004 0.0004 0.0014 0.0008
Bud Periphery 0.0003 0.001 0.0005 0.0003 0 0.0001 0.0017 0.0079 0.0008 0.001 0.0008 0.0055 0.0019 0.0003 0.0003 0 0.0033 0.0001
Bud Site 0.0004 0.005 0.0086 0.0003 0.0001 0.0001 0.0048 0.0166 0.0029 0.0089 0.0005 0.0018 0.0012 0.01 0.0008 0.0001 0.002 0.0001
Cell Periphery 0.0001 0.0003 0.0001 0.0001 0 0 0.0004 0.0003 0.0001 0.0004 0.0002 0.0002 0.0002 0.0001 0.0001 0 0.0002 0
Cytoplasm 0.6146 0.5833 0.6466 0.5161 0.5613 0.7022 0.4044 0.7368 0.5654 0.6334 0.7119 0.6837 0.5126 0.6006 0.5443 0.5452 0.4398 0.7069
Cytoplasmic Foci 0.0109 0.0076 0.0145 0.0536 0.0097 0.0251 0.0242 0.0075 0.0121 0.0308 0.0132 0.0158 0.0133 0.0103 0.0113 0.0055 0.0151 0.0095
Eisosomes 0 0.0001 0 0.0001 0 0.0001 0.0007 0 0.0001 0.0002 0 0.0001 0.0002 0 0.0001 0 0.0004 0
Endoplasmic Reticulum 0.0052 0.0051 0.0051 0.0041 0.0032 0.0043 0.007 0.0037 0.0031 0.011 0.0069 0.0045 0.0042 0.0032 0.0036 0.0018 0.0108 0.0032
Endosome 0.0055 0.0038 0.005 0.0191 0.006 0.0088 0.0312 0.0077 0.0061 0.0178 0.0055 0.0106 0.0108 0.0037 0.0125 0.0018 0.0339 0.0029
Golgi 0.0002 0.0002 0.0003 0.0004 0.0001 0.0005 0.0117 0.0005 0.0115 0.0026 0.0006 0.0029 0.0031 0.0003 0.0043 0.0001 0.0114 0.0002
Lipid Particles 0.0006 0.0002 0.0022 0.0019 0.0001 0.0013 0.0114 0.0002 0.0021 0.0018 0.0005 0.003 0.003 0.0006 0.0103 0 0.0042 0.0001
Mitochondria 0.0003 0.0003 0.0005 0.0006 0.0004 0.0005 0.0117 0.0005 0.002 0.0043 0.0004 0.0045 0.0015 0.0003 0.001 0.0004 0.0202 0.0003
None 0.3063 0.3594 0.2379 0.2924 0.3205 0.1752 0.3938 0.1693 0.3046 0.1631 0.2338 0.1866 0.3669 0.317 0.3586 0.3954 0.315 0.2446
Nuclear Periphery 0.0082 0.0048 0.0066 0.0053 0.0039 0.0078 0.0132 0.0026 0.0035 0.0137 0.0029 0.0063 0.0044 0.0021 0.0055 0.0019 0.014 0.0021
Nucleolus 0.0002 0.0003 0.0003 0.0011 0.0005 0.0005 0.0008 0.0003 0.0004 0.0004 0.0001 0.0004 0.0007 0.0003 0.0006 0.0001 0.0007 0.0001
Nucleus 0.0352 0.0207 0.0563 0.0777 0.08 0.0557 0.007 0.033 0.0301 0.0575 0.0132 0.0412 0.0169 0.0438 0.0304 0.0445 0.0788 0.022
Peroxisomes 0.006 0.0001 0.0004 0.0033 0.0001 0.0009 0.0061 0.0003 0.0111 0.0041 0.0003 0.0065 0.0027 0.0002 0.0052 0.0001 0.0023 0.0003
Punctate Nuclear 0.0028 0.0028 0.0096 0.0187 0.0118 0.0113 0.0108 0.0018 0.0137 0.0064 0.0043 0.0067 0.0048 0.0033 0.0041 0.0016 0.0079 0.004
Vacuole 0.0021 0.002 0.0016 0.0029 0.0011 0.0014 0.0029 0.0031 0.001 0.0018 0.0014 0.0012 0.0017 0.0014 0.0019 0.0006 0.0037 0.0007
Vacuole Periphery 0.0003 0.0002 0.0003 0.0004 0.0002 0.0004 0.0015 0.0002 0.0002 0.001 0.0003 0.0005 0.0005 0.0001 0.0005 0.0001 0.002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.1084 2.6095 4.8408 4.5894 0.9462 5.8349 6.5961 4.8971 6.9716 7.9148
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2214 280 2488 1892 878 2283 1815 1527 3092 2563 4303 3419

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 718.93 768.65 883.93 793.56 676.86 826.84 867.13 828.06 706.98 820.48 876.84 808.97
Standard Deviation 86.04 175.51 112.46 118.20 85.77 98.90 118.19 125.57 88.03 111.39 115.21 122.75
Intensity Change Log 2 — 0.096476 0.298081 0.142488 — 0.288751 0.357391 0.290878 — 0.192918 0.327147 0.216354

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000153 0.001031 0.000104 0.000326 0.000125 0.000233 0.000137 0.000857 0.000145 0.000320 0.000118 0.000563
Bud Neck 0.016006 0.025975 0.004785 0.008594 0.006211 0.038873 0.002259 0.005174 0.013224 0.037464 0.003720 0.007066
Bud Site 0.005600 0.023642 0.006904 0.020607 0.003884 0.014906 0.005941 0.030023 0.005113 0.015861 0.006498 0.024812
Cell Periphery 0.000145 0.000191 0.000081 0.000521 0.000377 0.000142 0.000118 0.000218 0.000211 0.000147 0.000097 0.000386
Cytoplasm 0.505053* 0.336409* 0.514404* 0.488144* 0.568333* 0.476333* 0.520104* 0.536614* 0.523022* 0.461046* 0.516808* 0.509792*
Cytoplasmic Foci 0.195710* 0.274887* 0.006135 0.024897 0.136349 0.188178* 0.015623 0.026319 0.178854 0.197650* 0.010137 0.025532
Eisosomes 0.000145 0.000139 0.000016 0.000221 0.000074 0.000119 0.000034 0.000042 0.000125 0.000121 0.000023 0.000141
Endoplasmic Reticulum 0.000865 0.001643 0.001408 0.001013 0.000905 0.000523 0.001773 0.001472 0.000876 0.000646 0.001562 0.001218
Endosome 0.008889 0.034244 0.001142 0.005529 0.005740 0.011959 0.002194 0.003029 0.007995 0.014393 0.001586 0.004412
Golgi 0.003217 0.005111 0.000049 0.001055 0.000685 0.002735 0.000560 0.001382 0.002498 0.002995 0.000265 0.001201
Lipid Particles 0.006580 0.007857 0.000478 0.001672 0.004703 0.004133 0.001019 0.001039 0.006047 0.004540 0.000706 0.001389
Mitochondria 0.004806 0.010401 0.000586 0.001871 0.001295 0.009050 0.000566 0.000811 0.003809 0.009198 0.000578 0.001398
Mitotic Spindle 0.002958 0.007073 0.006005 0.017213 0.000708 0.006240 0.005095 0.018223 0.002319 0.006331 0.005621 0.017664
None 0.002896 0.002359 0.006799 0.007527 0.008178 0.004194 0.008332 0.008425 0.004396 0.003994 0.007445 0.007928
Nuclear Periphery 0.000347 0.000434 0.001519 0.000960 0.000432 0.000294 0.001109 0.001092 0.000371 0.000309 0.001346 0.001019
Nuclear Periphery Foci 0.000358 0.001070 0.000556 0.000822 0.000661 0.000174 0.001236 0.001275 0.000444 0.000272 0.000843 0.001024
Nucleolus 0.001188 0.002965 0.000329 0.001149 0.001273 0.001522 0.000447 0.000780 0.001212 0.001680 0.000379 0.000984
Nucleus 0.202009* 0.148803* 0.410643* 0.313575* 0.234439* 0.176246* 0.379947* 0.272231* 0.211218* 0.173248* 0.397696* 0.295110*
Peroxisomes 0.004504 0.029373 0.000101 0.001107 0.001986 0.010082 0.000355 0.000551 0.003789 0.012189 0.000208 0.000859
Vacuole 0.037654 0.084367 0.037819 0.100556 0.023113 0.052549 0.052933 0.088623 0.033525 0.056025 0.044194 0.095226
Vacuole Periphery 0.000917 0.002026 0.000138 0.002640 0.000528 0.001516 0.000216 0.001823 0.000807 0.001572 0.000171 0.002275

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.91 0.25 -1.22 2.44 -1.30 -2.16 -1.82 -2.13 -1.70 -1.83 -3.87 -1.29 -2.42 -0.88 -2.23
Bud Neck -2.14 9.87 7.28 4.28 -2.57 -14.31 2.95 0.73 16.55 -5.03 -12.17 10.82 7.66 16.56 -4.40
Bud Site -3.80 -0.05 -4.97 1.69 -4.73 -7.41 -0.74 -7.04 -2.63 -6.67 -8.62 -0.26 -8.41 -1.17 -8.08
Cell Periphery -2.26 6.92 -4.44 -2.89 -6.16 1.13 1.25 0.99 -1.93 -3.97 1.05 1.97 -0.80 -4.54 -6.97
Cytoplasm 10.15 8.48 7.65 -6.10 -0.03 8.80 10.94 9.34 1.48 -1.42 8.65 12.66 10.84 1.48 -1.02
Cytoplasmic Foci -5.26 44.66 39.90 18.56 -13.02 -7.56 23.00 21.07 36.94 -5.11 -3.38 49.34 44.73 41.69 -12.83
Eisosomes 0.18 19.34 -0.15 -0.21 -3.76 -7.32 7.42 5.41 13.53 -3.14 0.55 19.73 0.97 0.80 -3.97
Endoplasmic Reticulum -2.01 -10.10 -4.01 0.43 4.08 2.96 -11.10 -7.83 -12.91 4.48 2.16 -14.94 -8.00 -9.81 5.88
Endosome -4.99 12.42 6.23 5.99 -6.08 -5.64 4.44 4.01 12.25 -1.06 -6.33 12.72 8.18 12.14 -5.98
Golgi -0.92 6.60 4.84 2.50 -2.52 -6.79 1.62 -0.21 4.48 -1.04 -0.94 6.56 4.56 6.12 -2.42
Lipid Particles -1.03 14.13 11.23 5.31 -6.00 0.77 5.00 5.05 10.81 0.19 3.28 14.27 12.54 11.08 -5.40
Mitochondria -1.90 6.41 5.06 3.27 -3.09 -9.10 3.48 3.07 10.04 -1.02 -5.80 6.82 5.71 10.08 -3.23
Mitotic Spindle -1.03 -2.85 -5.70 -2.20 -3.59 -4.47 -3.84 -6.33 -3.48 -3.94 -3.09 -3.85 -7.98 -4.84 -5.24
None 0.79 -6.92 -4.15 -3.58 0.46 2.99 0.93 1.20 -2.03 0.50 0.60 -4.01 -2.51 -2.67 0.65
Nuclear Periphery -1.80 -29.24 -15.94 -13.13 4.48 1.71 -13.00 -11.05 -20.20 1.65 1.76 -32.48 -20.97 -23.94 4.88
Nuclear Periphery Foci -4.30 -4.87 -3.06 0.62 -1.52 7.40 -7.41 -3.95 -7.75 1.56 3.58 -9.49 -5.06 -6.44 -0.53
Nucleolus -2.07 3.99 1.46 2.63 -3.09 -1.00 4.56 2.64 3.58 -1.42 -1.65 5.18 2.49 4.10 -3.32
Nucleus 4.18 -37.72 -13.67 -11.37 19.63 7.09 -18.60 -5.56 -15.28 14.18 7.41 -41.87 -14.95 -21.44 24.24
Peroxisomes -5.43 10.89 7.55 6.28 -3.07 -9.77 4.41 4.53 13.09 -0.37 -9.37 11.50 9.23 13.70 -2.98
Vacuole -6.52 -9.17 -25.70 -8.39 -19.84 -12.58 -17.33 -24.18 -18.86 -11.17 -11.00 -17.70 -34.55 -26.67 -22.36
Vacuole Periphery -3.22 8.23 -1.36 0.62 -3.03 -6.18 5.32 -1.26 1.07 -2.12 -4.83 9.09 -1.63 0.60 -3.70
ORF
Human Ortholog
Description Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
Localization
Cell Percentages cytoplasm (45%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YOR289W

YOR289W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yor289w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available