Standard name | |
---|---|
Human Ortholog | |
Description | Alpha-arrestin involved in ubiquitin-dependent endocytosis; regulates endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its targets; involved in the basal internalization and turnover of alpha-factor receptor Ste2p; recruits ubiquitin ligase Rsp5p to Ste2p via its 2 PPXY motifs; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 19 | 40 | 9 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 11 | 21 | 12 | 9 | 15 | 8 | 18 | 61 | 9 | 7 | 10 | 9 |
Bud | 1 | 18 | 5 | 1 | 3 | 3 | 7 | 1 | 2 | 5 | 0 | 2 | 6 | 9 | 4 | 0 | 2 | 0 | 1 | 10 | 0 | 4 | 3 | 2 |
Bud Neck | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 8 | 42 | 7 | 9 | 8 | 8 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 24 | 4 | 0 | 0 | 0 | 2 | 3 | 4 | 2 | 0 | 1 | 14 | 53 | 44 | 12 | 13 | 16 | 1 | 6 | 1 | 2 | 4 | 4 |
Cytoplasm | 93 | 256 | 61 | 32 | 119 | 37 | 128 | 111 | 117 | 93 | 12 | 47 | 127 | 272 | 285 | 113 | 79 | 90 | 78 | 213 | 53 | 86 | 162 | 196 |
Endoplasmic Reticulum | 7 | 55 | 1 | 0 | 1 | 0 | 3 | 2 | 0 | 1 | 0 | 0 | 12 | 6 | 11 | 7 | 4 | 4 | 20 | 32 | 6 | 25 | 31 | 29 |
Endosome | 12 | 4 | 5 | 1 | 5 | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 2 | 9 | 9 | 9 | 3 | 2 | 13 | 36 | 5 | 3 | 5 | 8 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 4 | 5 | 1 | 0 | 1 | 1 |
Mitochondria | 1 | 4 | 0 | 0 | 1 | 9 | 14 | 7 | 32 | 33 | 11 | 14 | 1 | 1 | 1 | 3 | 0 | 0 | 1 | 13 | 14 | 4 | 7 | 4 |
Nucleus | 0 | 2 | 1 | 0 | 2 | 0 | 3 | 8 | 1 | 4 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 1 | 2 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 6 | 6 | 2 | 4 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 6 | 2 | 1 | 3 | 17 | 3 | 6 | 13 | 8 |
Unique Cell Count | 128 | 357 | 78 | 33 | 129 | 42 | 142 | 124 | 142 | 116 | 21 | 59 | 154 | 306 | 314 | 147 | 109 | 110 | 155 | 451 | 110 | 158 | 254 | 280 |
Labelled Cell Count | 141 | 407 | 90 | 34 | 135 | 49 | 162 | 136 | 161 | 139 | 23 | 68 | 173 | 376 | 368 | 164 | 130 | 127 | 155 | 451 | 110 | 158 | 254 | 280 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.2 | 7.8 | 7.0 | 5.9 | 7.2 | 5.7 | 5.8 | 6.1 | 5.2 | 5.1 | 4.7 | 5.1 | 7.3 | 7.5 | 7.5 | 10.4 | 10.9 | 9.8 | 7.0 | 7.1 | 7.3 |
Std Deviation (1e-4) | 0.8 | 0.9 | 1.1 | 0.8 | 1.5 | 1.6 | 1.3 | 1.6 | 1.2 | 1.4 | 0.7 | 0.9 | 0.9 | 1.0 | 1.1 | 1.4 | 1.5 | 1.3 | 1.0 | 1.1 | 1.0 |
Intensity Change (Log2) | – | – | – | -0.26 | 0.03 | -0.31 | -0.29 | -0.22 | -0.43 | -0.46 | -0.59 | -0.46 | 0.04 | 0.08 | 0.08 | 0.56 | 0.63 | 0.48 | -0.02 | 0.01 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 | -1.4 | 0 | -1.4 | 0.4 | -1.0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 2.7 | 2.1 | 0.8 | 1.6 | 2.1 |
Cytoplasm | 2.5 | 2.9 | 1.3 | 2.4 | 2.2 | 0.8 | 0.3 | -1.9 | 0.2 | 0.8 | 2.5 | 3.1 | -0.2 | -0.9 | 0.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.5 | 5.2 | 0 | 4.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.2864 | 6.3748 | 5.7861 | 5.694 | 5.1097 | 5.8992 | 3.9443 | 5.0285 | 4.7145 | 4.3621 | 4.2743 | 4.6512 | 3.58 | 3.9617 | 3.8067 | 3.6433 | 3.4511 | 3.7962 |
Actin | 0.0632 | 0.0469 | 0.0375 | 0.0091 | 0.0438 | 0.0397 | 0.0833 | 0.0503 | 0.0916 | 0.0596 | 0.0948 | 0.0807 | 0.0469 | 0.0239 | 0.0739 | 0.0135 | 0.0798 | 0.0792 |
Bud | 0.0014 | 0.0017 | 0.0022 | 0.0003 | 0.001 | 0.0017 | 0.0015 | 0.0059 | 0.0028 | 0.0022 | 0.0017 | 0.0018 | 0.0006 | 0.0011 | 0.001 | 0.0006 | 0.0011 | 0.0007 |
Bud Neck | 0.0137 | 0.0093 | 0.0162 | 0.0049 | 0.0719 | 0.0707 | 0.0068 | 0.0058 | 0.0152 | 0.021 | 0.0055 | 0.0752 | 0.0034 | 0.0038 | 0.0162 | 0.0586 | 0.0074 | 0.0524 |
Bud Periphery | 0.0029 | 0.002 | 0.0053 | 0.0004 | 0.0034 | 0.0038 | 0.002 | 0.0027 | 0.0066 | 0.004 | 0.0022 | 0.0026 | 0.0012 | 0.0008 | 0.0013 | 0.0007 | 0.0013 | 0.0019 |
Bud Site | 0.0271 | 0.1427 | 0.0366 | 0.0011 | 0.0046 | 0.0037 | 0.0158 | 0.097 | 0.0274 | 0.0194 | 0.0026 | 0.0032 | 0.0242 | 0.0985 | 0.0519 | 0.0025 | 0.0101 | 0.0012 |
Cell Periphery | 0.0254 | 0.0206 | 0.0152 | 0.0022 | 0.0399 | 0.0109 | 0.0225 | 0.0131 | 0.0176 | 0.0091 | 0.0082 | 0.0103 | 0.0555 | 0.0164 | 0.0113 | 0.0057 | 0.0098 | 0.0133 |
Cytoplasm | 0.3798 | 0.4407 | 0.4895 | 0.691 | 0.5909 | 0.521 | 0.4394 | 0.5329 | 0.4758 | 0.4589 | 0.7326 | 0.4919 | 0.4644 | 0.5089 | 0.579 | 0.5725 | 0.483 | 0.6067 |
Cytoplasmic Foci | 0.1503 | 0.1312 | 0.1311 | 0.1993 | 0.1028 | 0.1629 | 0.1417 | 0.155 | 0.158 | 0.1284 | 0.0738 | 0.1464 | 0.1002 | 0.2004 | 0.0903 | 0.131 | 0.0616 | 0.0964 |
Eisosomes | 0.0015 | 0.0004 | 0.0007 | 0 | 0.0006 | 0.0003 | 0.0011 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0003 |
Endoplasmic Reticulum | 0.0168 | 0.0082 | 0.0118 | 0.0042 | 0.0197 | 0.0138 | 0.0152 | 0.0076 | 0.0108 | 0.0143 | 0.0067 | 0.01 | 0.0288 | 0.0066 | 0.0178 | 0.0065 | 0.0719 | 0.028 |
Endosome | 0.1061 | 0.0665 | 0.1055 | 0.0241 | 0.0543 | 0.0544 | 0.0862 | 0.0485 | 0.0675 | 0.1179 | 0.0279 | 0.056 | 0.0547 | 0.0431 | 0.0465 | 0.1267 | 0.0451 | 0.0454 |
Golgi | 0.0553 | 0.0393 | 0.0445 | 0.0103 | 0.025 | 0.0437 | 0.0549 | 0.0383 | 0.0468 | 0.0593 | 0.0279 | 0.0588 | 0.0264 | 0.0216 | 0.0273 | 0.0553 | 0.0135 | 0.0304 |
Lipid Particles | 0.0645 | 0.0415 | 0.0378 | 0.0045 | 0.0094 | 0.0243 | 0.0744 | 0.0271 | 0.047 | 0.0272 | 0.0082 | 0.0344 | 0.0949 | 0.038 | 0.0212 | 0.0136 | 0.02 | 0.022 |
Mitochondria | 0.0193 | 0.0029 | 0.0045 | 0.0003 | 0.0018 | 0.0088 | 0.0093 | 0.0007 | 0.0026 | 0.0398 | 0.0014 | 0.0099 | 0.0013 | 0.0004 | 0.001 | 0.0007 | 0.0018 | 0.0012 |
None | 0.0397 | 0.0196 | 0.0302 | 0.0024 | 0.0071 | 0.0087 | 0.0141 | 0.0025 | 0.0067 | 0.0046 | 0.0012 | 0.0022 | 0.0631 | 0.0045 | 0.0306 | 0.001 | 0.0033 | 0.0026 |
Nuclear Periphery | 0.0026 | 0.0006 | 0.0019 | 0.0003 | 0.0081 | 0.0014 | 0.0064 | 0.0003 | 0.0005 | 0.0037 | 0.0002 | 0.0004 | 0.0056 | 0.0004 | 0.0145 | 0.0004 | 0.1422 | 0.0094 |
Nucleolus | 0.0009 | 0.0004 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0007 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0007 | 0.0001 |
Nucleus | 0.0011 | 0.0008 | 0.0012 | 0.0004 | 0.0017 | 0.0032 | 0.0023 | 0.0005 | 0.0006 | 0.0008 | 0.0003 | 0.0004 | 0.0013 | 0.0009 | 0.0046 | 0.0003 | 0.0278 | 0.0006 |
Peroxisomes | 0.0169 | 0.0119 | 0.0151 | 0.0422 | 0.0022 | 0.0202 | 0.008 | 0.0064 | 0.0152 | 0.0151 | 0.0026 | 0.0108 | 0.0058 | 0.0171 | 0.0033 | 0.0024 | 0.0022 | 0.0044 |
Punctate Nuclear | 0.0019 | 0.0009 | 0.0022 | 0.0016 | 0.0018 | 0.0008 | 0.0071 | 0.0007 | 0.0009 | 0.0008 | 0.0003 | 0.0007 | 0.0015 | 0.0012 | 0.0017 | 0.0006 | 0.0072 | 0.0007 |
Vacuole | 0.0083 | 0.0105 | 0.0096 | 0.0013 | 0.0089 | 0.0054 | 0.0064 | 0.0039 | 0.0051 | 0.0101 | 0.0015 | 0.0033 | 0.019 | 0.0114 | 0.0055 | 0.0066 | 0.0056 | 0.0028 |
Vacuole Periphery | 0.0015 | 0.0014 | 0.001 | 0.0001 | 0.0009 | 0.0006 | 0.001 | 0.0002 | 0.0006 | 0.0036 | 0.0002 | 0.0006 | 0.0008 | 0.0003 | 0.0006 | 0.0006 | 0.0046 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.658 | 17.2626 | 14.8949 | 22.4528 | 19.7968 | 15.2605 | 21.4118 | 23.4238 | 21.9515 | 22.5147 |
Translational Efficiency | 0.5624 | 0.7401 | 0.8193 | 0.5365 | 0.5658 | 0.9066 | 0.6246 | 0.6476 | 0.5836 | 0.5854 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
954 | 1593 | 982 | 1268 | 1802 | 1974 | 209 | 1432 | 2756 | 3567 | 1191 | 2700 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 799.63 | 833.00 | 1067.88 | 923.76 | 852.44 | 871.80 | 1077.62 | 936.62 | 834.16 | 854.47 | 1069.59 | 930.58 |
Standard Deviation | 85.07 | 137.72 | 131.54 | 117.94 | 98.33 | 105.69 | 104.63 | 117.70 | 97.25 | 122.57 | 127.28 | 117.98 |
Intensity Change Log 2 | — | 0.058984 | 0.417345 | 0.208185 | — | 0.032399 | 0.338178 | 0.135866 | — | 0.045328 | 0.377039 | 0.171323 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.009731 | 0.013681 | 0.055055 | 0.006278 | 0.018424 | 0.006677 | 0.060114 | 0.011669 | 0.015415 | 0.009805 | 0.055942 | 0.009137 |
Bud Neck | 0.068408 | 0.021828 | 0.004429 | 0.011026 | 0.029829 | 0.035218 | 0.010807 | 0.010266 | 0.043183 | 0.029238 | 0.005549 | 0.010623 |
Bud Site | 0.065533 | 0.042566 | 0.037548 | 0.054323 | 0.065942 | 0.040693 | 0.071521 | 0.069949 | 0.065800 | 0.041529 | 0.043509 | 0.062611 |
Cell Periphery | 0.007087 | 0.000362 | 0.000186 | 0.000188 | 0.001593 | 0.000686 | 0.000798 | 0.000237 | 0.003495 | 0.000541 | 0.000293 | 0.000214 |
Cytoplasm | 0.432479* | 0.253660* | 0.024156 | 0.265631* | 0.342324* | 0.282731* | 0.089599 | 0.287058* | 0.373531* | 0.269748* | 0.035640 | 0.276995* |
Cytoplasmic Foci | 0.208700* | 0.390400* | 0.360520* | 0.209663* | 0.318935* | 0.338425* | 0.330064* | 0.203092* | 0.280777* | 0.361637* | 0.355175* | 0.206178* |
Eisosomes | 0.009942 | 0.001250 | 0.000163 | 0.000183 | 0.003663 | 0.002041 | 0.000739 | 0.000237 | 0.005836 | 0.001688 | 0.000264 | 0.000212 |
Endoplasmic Reticulum | 0.004173 | 0.001825 | 0.004949 | 0.001286 | 0.003840 | 0.001471 | 0.004223 | 0.001633 | 0.003956 | 0.001629 | 0.004822 | 0.001470 |
Endosome | 0.037712 | 0.079409 | 0.156377 | 0.055630 | 0.042156 | 0.088569 | 0.092211 | 0.040188 | 0.040618 | 0.084478 | 0.145117 | 0.047440 |
Golgi | 0.064388 | 0.073110 | 0.078358 | 0.007454 | 0.089130 | 0.070780 | 0.010952 | 0.008469 | 0.080565 | 0.071821 | 0.066529 | 0.007992 |
Lipid Particles | 0.015269 | 0.014947 | 0.055611 | 0.006791 | 0.018380 | 0.014342 | 0.026868 | 0.007157 | 0.017303 | 0.014612 | 0.050567 | 0.006985 |
Mitochondria | 0.003668 | 0.005522 | 0.000316 | 0.003766 | 0.004032 | 0.007823 | 0.000059 | 0.003029 | 0.003906 | 0.006796 | 0.000271 | 0.003375 |
Mitotic Spindle | 0.000571 | 0.004567 | 0.126084* | 0.027289 | 0.000725 | 0.003660 | 0.007048 | 0.040032 | 0.000672 | 0.004065 | 0.105195* | 0.034048 |
None | 0.000399 | 0.001261 | 0.000026 | 0.000438 | 0.000432 | 0.000275 | 0.000063 | 0.001393 | 0.000421 | 0.000715 | 0.000033 | 0.000945 |
Nuclear Periphery | 0.000047 | 0.000075 | 0.000246 | 0.000361 | 0.000026 | 0.000048 | 0.000163 | 0.000422 | 0.000033 | 0.000060 | 0.000231 | 0.000394 |
Nuclear Periphery Foci | 0.000585 | 0.000713 | 0.009325 | 0.002511 | 0.000585 | 0.000363 | 0.008412 | 0.002504 | 0.000585 | 0.000519 | 0.009165 | 0.002507 |
Nucleolus | 0.000117 | 0.001196 | 0.000043 | 0.000455 | 0.000138 | 0.000233 | 0.000080 | 0.000271 | 0.000130 | 0.000663 | 0.000049 | 0.000357 |
Nucleus | 0.006641 | 0.006441 | 0.000934 | 0.020921 | 0.005776 | 0.006598 | 0.005520 | 0.015084 | 0.006075 | 0.006528 | 0.001738 | 0.017825 |
Peroxisomes | 0.021855 | 0.032198 | 0.047105 | 0.055450 | 0.022120 | 0.028096 | 0.077934 | 0.057995 | 0.022028 | 0.029928 | 0.052515 | 0.056800 |
Vacuole | 0.041209 | 0.051921 | 0.037893 | 0.267813* | 0.030247 | 0.068122 | 0.202501* | 0.236945* | 0.034042 | 0.060886 | 0.066779 | 0.251442* |
Vacuole Periphery | 0.001487 | 0.003069 | 0.000677 | 0.002542 | 0.001702 | 0.003149 | 0.000325 | 0.002371 | 0.001628 | 0.003113 | 0.000615 | 0.002451 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.23 | -10.51 | 5.58 | 5.62 | 11.90 | 7.32 | -3.67 | 6.03 | -0.74 | 4.82 | 4.48 | -9.79 | 7.94 | 3.70 | 12.45 |
Bud Neck | 12.11 | 17.18 | 15.49 | 8.37 | -6.87 | -2.62 | 10.57 | 14.01 | 16.86 | 0.74 | 7.47 | 22.60 | 20.15 | 18.41 | -7.13 |
Bud Site | 5.76 | 6.03 | 5.31 | 0.33 | -0.47 | 8.58 | -0.39 | 3.77 | -3.00 | 2.58 | 10.30 | 6.93 | 6.61 | -2.04 | -0.64 |
Cell Periphery | 8.21 | 8.41 | 8.47 | 7.31 | 2.57 | 4.74 | 5.18 | 11.98 | 3.30 | 5.82 | 9.54 | 10.70 | 11.17 | 4.37 | 5.63 |
Cytoplasm | 17.11 | 45.70 | 14.76 | -0.97 | -28.60 | 7.31 | 22.49 | 6.03 | -0.29 | -16.13 | 16.11 | 58.44 | 13.56 | -0.78 | -38.89 |
Cytoplasmic Foci | -22.37 | -15.22 | 4.67 | 28.82 | 20.31 | -2.85 | 0.05 | 22.51 | 25.40 | 10.18 | -15.23 | -8.43 | 21.32 | 38.03 | 24.19 |
Eisosomes | 10.58 | 11.99 | 12.01 | 10.42 | 0.81 | 5.18 | 12.41 | 15.44 | 8.45 | 6.32 | 11.92 | 17.23 | 17.53 | 11.62 | 4.50 |
Endoplasmic Reticulum | 2.24 | -0.83 | 2.80 | 1.95 | 4.27 | 4.86 | -0.81 | 4.97 | 0.15 | 4.99 | 4.81 | -1.33 | 5.48 | 1.41 | 4.83 |
Endosome | -11.30 | -21.74 | -0.53 | 10.54 | 21.11* | -14.12 | -4.81 | 5.09 | 17.76 | 6.23 | -17.94 | -22.27 | 3.16 | 20.02 | 23.53 |
Golgi | -1.91 | -1.87 | 18.09 | 23.20 | 19.13* | 4.88 | 24.11 | 28.76 | 24.35 | 2.48 | 3.18 | 5.01 | 33.89 | 33.62 | 18.91* |
Lipid Particles | 0.15 | -11.73 | 6.66 | 8.43 | 15.43 | 2.79 | -1.97 | 9.70 | 7.38 | 5.45 | 2.43 | -11.63 | 12.02 | 11.04 | 16.12 |
Mitochondria | -2.31 | 7.03 | 1.53 | 3.14 | -2.22 | -4.28 | 11.06 | 3.39 | 6.16 | -3.41 | -4.77 | 12.42 | 3.36 | 6.64 | -3.56 |
Mitotic Spindle | -4.13 | -17.01 | -9.43 | -7.87 | 12.02 | -3.50 | -2.49 | -10.23 | -9.09 | -6.59 | -5.34 | -16.76 | -13.89 | -12.03 | 10.95 |
None | -1.50 | 2.70 | -1.08 | 1.23 | -7.09 | 1.67 | 4.94 | -1.64 | -2.03 | -2.63 | -1.16 | 5.90 | -1.92 | -0.34 | -3.87 |
Nuclear Periphery | -1.58 | -6.42 | -15.30 | -14.23 | -6.12 | -3.17 | -6.47 | -9.17 | -8.76 | -5.43 | -3.19 | -7.77 | -15.86 | -14.93 | -6.30 |
Nuclear Periphery Foci | -0.77 | -6.28 | -7.80 | -8.08 | 4.48 | 1.18 | -7.01 | -7.58 | -7.57 | 4.91 | 0.28 | -7.37 | -11.14 | -10.99 | 5.36 |
Nucleolus | -2.58 | 6.29 | -4.60 | 0.84 | -4.97 | -2.28 | 4.34 | -7.23 | -2.00 | -8.78 | -2.85 | 8.83 | -5.94 | 0.49 | -6.84 |
Nucleus | 0.12 | 10.08 | -12.66 | -12.42 | -15.73 | -1.58 | -0.96 | -13.38 | -12.02 | -9.39 | -1.29 | 8.84 | -18.35 | -17.20 | -21.15 |
Peroxisomes | -4.55 | -7.16 | -5.09 | -2.00 | 1.81 | -3.92 | -6.54 | -6.50 | -4.33 | 3.79 | -6.12 | -9.68 | -8.26 | -4.52 | 3.11 |
Vacuole | -3.56 | -1.73 | -36.82 | -35.38 | -34.33 | -14.08 | -12.73 | -40.09 | -33.76 | -5.58 | -13.44 | -10.11 | -54.82 | -48.74 | -36.75 |
Vacuole Periphery | -5.45 | 5.94 | 0.07 | 3.41 | -2.01 | -5.81 | 9.55 | 0.75 | 4.01 | -2.80 | -7.69 | 8.37 | 0.70 | 5.27 | -2.91 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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