Standard name
Human Ortholog
Description Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 – 0 0 0 0 – 0 0 0 0 – – – 0.06 0.05 0.05 0.13 0.14 0 0 0 0
Bud 0 0 – 0 0 0.06 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0.07 0.05 0 0.07 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 – 0.06 0 0 0 – 0 0 0 0 – – – 0 0 0 0.05 0.08 0.09 0 0 0
Cytoplasm 0.77 0.79 – 0.94 0.87 0.81 0.82 – 0.42 0.55 0.45 0.55 – – – 0.27 0.25 0.26 0.24 0.22 0.28 0 0.33 0.22
Endoplasmic Reticulum 0 0.13 – 0 0.06 0 0.05 – 0 0 0 0.05 – – – 0.11 0.07 0.05 0.19 0.21 0.1 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0.32 0.29 0.4 0 0 0 0 0.05 0.18
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0 – 0 0 0.16 0.19 – 0.62 0.5 0.69 0.57 – – – 0.07 0.07 0.13 0.21 0.16 0.28 0.17 0.17 0.19
Nucleus 0 0 – 0 0 0 0 – 0 0.05 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0.09 0.09 0.09 0 0 0 0 0.1 0.09
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0.26 0.34 0.2 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0.05 0.08 0.05 0.06 0.08 0 0 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 11 – 0 2 1 0 – 0 0 0 0 – – – 9 7 6 23 41 1 0 0 0
Bud 6 2 – 0 1 4 0 – 1 0 2 2 – – – 5 3 1 1 1 1 0 0 0
Bud Neck 0 2 – 0 1 0 0 – 0 1 0 0 – – – 1 2 0 5 19 1 0 0 0
Bud Site 1 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 2 9 – 1 3 2 3 – 0 0 2 0 – – – 1 1 3 9 21 3 0 0 0
Cytoplasm 111 178 – 17 69 57 70 – 11 24 30 33 – – – 39 37 33 41 62 11 0 2 3
Endoplasmic Reticulum 6 29 – 0 5 3 4 – 0 1 2 3 – – – 16 11 7 33 61 4 0 0 0
Endosome 4 7 – 0 0 0 1 – 0 1 1 0 – – – 47 43 51 5 10 0 0 0 2
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – – – 3 4 2 4 2 0 0 0 0
Mitochondria 25 2 – 0 3 11 16 – 16 22 46 34 – – – 11 10 17 36 46 10 0 1 2
Nucleus 0 1 – 0 0 0 1 – 1 2 1 2 – – – 0 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 – 0 0 0 0 – 0 0 0 1 – – – 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 13 14 11 0 1 0 0 0 1
SpindlePole 1 2 – 0 1 0 1 – 0 1 0 0 – – – 38 51 25 0 0 0 0 0 0
Vac/Vac Membrane 0 1 – 0 0 0 0 – 0 0 0 0 – – – 3 8 10 9 17 2 0 0 1
Unique Cell Count 144 226 18 79 70 85 26 44 67 60 147 149 128 175 291 39 2 8 14
Labelled Cell Count 160 244 18 85 78 96 29 52 84 75 186 191 168 175 291 39 2 8 14


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.4 – 6.8 6.3 5.0 5.1 – 4.6 4.6 4.3 4.5 – – – 7.1 7.0 6.7 – – –
Std Deviation (1e-4) 0.7 0.9 – 2.8 2.4 1.3 1.3 – 1.9 1.4 1.6 1.5 – – – 1.3 1.5 1.4 – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4602 -0.3276 -0.3068 -0.0541 -0.4974 -0.3432 -0.2372 0.0794 0.1033 0.0592 -0.5359 -0.2388 -0.1728 -0.409 -0.938 -0.8292 0.4363 -0.271
Actin 0.0169 0.0055 0.0235 0.0194 0.0089 0.0047 0.056 0.0024 0.0176 0.0332 0.0442 0.0038 0.085 0.0221 0.0186 0.0727 0.0507 0.0057
Bud 0.0035 0.0041 0.0012 0.0004 0.0007 0.0039 0.0014 0.0018 0.0006 0.0004 0.0012 0.0001 0.0038 0.0005 0.0011 0.0004 0.004 0.0002
Bud Neck 0.0023 0.002 0.0073 0.003 0.0019 0.0048 0.0019 0.0009 0.0087 0.0011 0.0025 0.0043 0.0008 0.0006 0.0022 0.0041 0.0014 0.0352
Bud Periphery 0.0042 0.002 0.003 0.0006 0.0016 0.003 0.0032 0.0041 0.002 0.0007 0.0029 0.0005 0.0025 0.0003 0.0014 0.0006 0.0071 0.0005
Bud Site 0.0073 0.0198 0.0086 0.0012 0.0034 0.001 0.0066 0.0165 0.0082 0.0013 0.0172 0.0013 0.0035 0.0114 0.0168 0.0009 0.0069 0.0005
Cell Periphery 0.0022 0.0012 0.0013 0.0002 0.0004 0.0006 0.0036 0.004 0.0031 0.0004 0.0012 0.0005 0.0006 0.002 0.001 0.0004 0.0003 0.0004
Cytoplasm 0.0887 0.1809 0.1504 0.07 0.1427 0.1933 0.0932 0.1273 0.1148 0.0602 0.1407 0.163 0.0828 0.195 0.1985 0.1923 0.0447 0.2216
Cytoplasmic Foci 0.1205 0.1008 0.0874 0.0651 0.0705 0.0811 0.1051 0.1048 0.0789 0.0761 0.1723 0.1448 0.1428 0.1077 0.1326 0.1519 0.0458 0.1076
Eisosomes 0.0012 0.0005 0.0009 0.0004 0.0004 0.0003 0.0018 0.0006 0.0007 0.0007 0.0013 0.0003 0.0011 0.0004 0.0006 0.0003 0.0005 0.0002
Endoplasmic Reticulum 0.0083 0.0087 0.0103 0.0033 0.0099 0.0065 0.0096 0.0068 0.0114 0.0046 0.0054 0.0057 0.0081 0.0271 0.0103 0.0101 0.0039 0.021
Endosome 0.0751 0.055 0.0553 0.0793 0.0515 0.0351 0.0472 0.0401 0.036 0.0494 0.0445 0.031 0.0966 0.0627 0.0945 0.0516 0.0293 0.0477
Golgi 0.0156 0.0094 0.0181 0.033 0.0183 0.0082 0.0107 0.0047 0.0084 0.0167 0.0159 0.0049 0.0366 0.0095 0.0232 0.0158 0.0606 0.0074
Lipid Particles 0.0411 0.0296 0.0257 0.038 0.0146 0.017 0.0747 0.0359 0.0338 0.0336 0.0352 0.0418 0.0759 0.0352 0.0782 0.0397 0.0294 0.0534
Mitochondria 0.0201 0.0026 0.0188 0.0508 0.0254 0.0115 0.0071 0.0118 0.0076 0.0267 0.0093 0.0029 0.0695 0.0011 0.0353 0.0086 0.0404 0.1235
None 0.53 0.5444 0.5413 0.6107 0.616 0.5958 0.5223 0.6151 0.6248 0.6555 0.3946 0.5768 0.295 0.5043 0.3559 0.3953 0.6479 0.3571
Nuclear Periphery 0.0069 0.0017 0.0044 0.0012 0.0052 0.0048 0.0077 0.0013 0.002 0.0102 0.0022 0.0012 0.0152 0.0016 0.002 0.0015 0.002 0.0013
Nucleolus 0.0026 0.0009 0.0023 0.0003 0.0003 0.0009 0.0026 0.001 0.0014 0.0009 0.0018 0.0003 0.0013 0.0004 0.0007 0.0002 0.0006 0.0003
Nucleus 0.0038 0.002 0.0035 0.0006 0.0011 0.0041 0.0027 0.0012 0.0017 0.0019 0.0016 0.0006 0.0017 0.0006 0.0017 0.0006 0.0007 0.0004
Peroxisomes 0.0266 0.0206 0.0172 0.0181 0.0088 0.0106 0.0259 0.0083 0.0246 0.017 0.0816 0.0099 0.0621 0.0104 0.0085 0.0476 0.0185 0.0058
Punctate Nuclear 0.0035 0.0032 0.0139 0.0016 0.0008 0.0077 0.0092 0.0021 0.0071 0.0061 0.0208 0.0035 0.009 0.0026 0.0032 0.0029 0.0028 0.0015
Vacuole 0.0146 0.0046 0.0043 0.002 0.0113 0.0037 0.0064 0.0088 0.0056 0.0024 0.003 0.0026 0.0039 0.0041 0.0122 0.0021 0.0017 0.0069
Vacuole Periphery 0.0047 0.0005 0.0012 0.0009 0.0063 0.0015 0.0011 0.0009 0.0009 0.001 0.0007 0.0004 0.0022 0.0005 0.0016 0.0003 0.0008 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.7592 8.7242 9.7106 11.59 12.6533 9.1442 12.6802 13.5816 12.3338 14.4954
Translational Efficiency 0.6277 0.6826 0.5436 0.5132 0.457 0.6244 0.3597 0.4236 0.41 0.3436

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
169 1853 1148 1003 2046 2319 2333 1720 2215 4172 3481 2723

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 657.40 755.38 950.44 852.46 715.23 723.79 940.91 965.53 710.82 737.82 944.05 923.88
Standard Deviation 108.63 91.05 124.28 108.94 79.81 89.30 101.27 133.04 83.79 91.44 109.48 136.11
Intensity Change Log 2 — 0.200431 0.531824 0.374861 — 0.017164 0.395649 0.432914 — 0.107845 0.462474 0.405401

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.008336 0.010455 0.001240 0.005579 0.003110 0.002361 0.001849 0.003353 0.003509 0.005956 0.001648 0.004173
Bud Neck 0.061380 0.025900 0.004617 0.014920 0.019087 0.033767 0.011882 0.056576 0.022314 0.030273 0.009486 0.041232
Bud Site 0.018299 0.017798 0.016439 0.046001 0.004162 0.014295 0.005440 0.042711 0.005241 0.015851 0.009067 0.043923
Cell Periphery 0.001138 0.000439 0.000673 0.000572 0.000647 0.000385 0.000343 0.000231 0.000685 0.000409 0.000452 0.000357
Cytoplasm 0.111554* 0.233935* 0.489038* 0.310738* 0.359213* 0.263964* 0.491120* 0.428846* 0.340317* 0.250627* 0.490434* 0.385342*
Cytoplasmic Foci 0.253833* 0.381559* 0.046391 0.156834 0.295925* 0.348786* 0.066018 0.075454 0.292714* 0.363342* 0.059545 0.105430
Eisosomes 0.002498 0.002704 0.000122 0.000420 0.003617 0.001468 0.000317 0.000159 0.003532 0.002017 0.000253 0.000255
Endoplasmic Reticulum 0.005417 0.004998 0.015322 0.005855 0.005356 0.004112 0.006319 0.001274 0.005361 0.004505 0.009288 0.002962
Endosome 0.082638 0.040088 0.005280 0.037408 0.015331 0.052704 0.006286 0.027861 0.020467 0.047101 0.005954 0.031378
Golgi 0.053236 0.050772 0.000389 0.005726 0.027986 0.029881 0.000502 0.002182 0.029912 0.039160 0.000465 0.003487
Lipid Particles 0.148121* 0.054035 0.004580 0.030783 0.070685 0.065939 0.013878 0.015900 0.076593 0.060652 0.010812 0.021382
Mitochondria 0.033677 0.019490 0.000329 0.001833 0.007902 0.018043 0.000724 0.010126 0.009869 0.018685 0.000594 0.007071
Mitotic Spindle 0.003845 0.002494 0.001464 0.026738 0.000324 0.005114 0.002674 0.060183 0.000593 0.003950 0.002275 0.047864
None 0.001809 0.001235 0.001290 0.001494 0.002582 0.001976 0.001612 0.002920 0.002523 0.001647 0.001505 0.002395
Nuclear Periphery 0.000551 0.000239 0.001114 0.001531 0.000431 0.000549 0.002491 0.002517 0.000441 0.000411 0.002037 0.002154
Nuclear Periphery Foci 0.001121 0.000915 0.014798 0.007900 0.000628 0.000864 0.005401 0.001418 0.000665 0.000887 0.008500 0.003805
Nucleolus 0.001472 0.000741 0.000210 0.001468 0.000817 0.001230 0.000652 0.003002 0.000867 0.001013 0.000506 0.002437
Nucleus 0.034846 0.043602 0.262534* 0.102849 0.122293 0.055668 0.293710* 0.121656 0.115621 0.050309 0.283428* 0.114729
Peroxisomes 0.137346* 0.063979 0.000633 0.022215 0.028409 0.033223 0.006901 0.024529 0.036721 0.046883 0.004834 0.023676
Vacuole 0.035234 0.042377 0.133163 0.217812* 0.030680 0.063008 0.081799 0.118155 0.031027 0.053845 0.098738 0.154863
Vacuole Periphery 0.003649 0.002245 0.000374 0.001324 0.000813 0.002664 0.000082 0.000947 0.001029 0.002478 0.000178 0.001086

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.59 2.01 1.21 5.06 -2.70 1.50 2.69 1.10 -0.21 -1.38 -3.93 3.77 0.78 4.77 -2.83
Bud Neck 3.24 5.16 4.30 6.73 -8.49 -7.11 4.19 -12.74 -6.41 -16.21 -4.42 7.80 -7.54 -4.10 -17.12
Bud Site 0.09 0.33 -2.74 -4.52 -4.63 -8.31 -1.40 -11.81 -6.87 -10.99 -10.54 -4.06 -13.57 -8.05 -11.40
Cell Periphery 3.17 2.01 3.13 -0.22 5.10 4.33 8.01 12.91 3.74 4.75 5.94 6.00 10.85 3.67 6.39
Cytoplasm -8.72 -22.55 -11.32 -5.28 14.65 13.25 -13.73 -3.97 -14.95 7.54 14.28 -17.58 -1.89 -15.61 14.70
Cytoplasmic Foci -8.20 14.14 7.93 34.37 -15.25 -8.12 43.27 39.45 49.14 -1.96 -12.66 48.68 37.56 60.51 -10.75
Eisosomes -0.38 5.45 4.97 8.99 -6.43 8.32 14.18 14.93 11.31 4.51 6.00 15.08 15.25 13.44 1.08
Endoplasmic Reticulum 0.21 -5.01 -0.11 -0.99 11.51 1.85 -2.09 6.84 6.05 11.43 1.40 -6.88 4.18 3.91 15.43
Endosome 4.50 8.37 5.82 4.30 -9.41 -17.25 8.41 -4.83 10.84 -9.70 -15.01 12.16 -2.82 11.11 -13.31
Golgi 0.35 7.16 6.67 19.15 -2.90 -0.82 19.70 18.83 19.16 -6.01 -4.71 20.85 18.82 27.23 -4.40
Lipid Particles 5.88 9.08 7.81 9.07 -8.92 1.33 18.78 18.37 18.09 -0.34 4.76 22.08 19.07 19.92 -5.14
Mitochondria 2.32 5.59 5.40 12.23 -3.83 -7.27 9.26 0.04 7.42 -9.67 -7.01 10.81 4.50 12.82 -10.15
Mitotic Spindle 0.60 1.11 -5.39 -6.53 -6.85 -5.11 -4.91 -13.58 -11.98 -12.49 -5.43 -4.43 -15.21 -13.71 -14.14
None 0.61 0.76 0.34 -0.50 -1.07 1.12 2.26 0.12 -1.23 -2.81 1.90 2.75 0.90 -1.45 -2.86
Nuclear Periphery 2.40 -5.38 -7.80 -12.00 -5.06 -1.50 -13.40 -10.79 -10.28 -2.22 0.45 -14.88 -13.02 -13.12 -2.97
Nuclear Periphery Foci 0.75 -13.87 -9.62 -10.54 6.46 -2.12 -9.29 -3.42 -2.57 6.85 -2.77 -15.65 -9.99 -9.06 8.88
Nucleolus 1.48 2.57 -0.34 -1.92 -2.99 -3.11 -2.22 -8.54 -7.53 -6.67 -1.29 -0.61 -7.58 -7.30 -7.00
Nucleus -0.87 -22.28 -8.31 -13.60 18.78 18.40 -29.77 -3.17 -17.86 23.59 20.10 -34.56 -3.31 -22.69 30.26
Peroxisomes 4.85 9.20 8.11 14.71 -8.54 -2.29 12.64 3.61 5.90 -7.26 -4.20 16.14 7.92 14.63 -10.24
Vacuole -1.27 -16.26 -24.81 -28.26 -12.38 -12.82 -22.07 -26.94 -19.17 -9.99 -12.27 -30.45 -38.22 -32.91 -15.91
Vacuole Periphery 1.24 3.00 2.49 3.80 -2.15 -9.60 8.98 0.97 10.44 -9.31 -7.29 7.22 1.83 9.21 -6.11
Standard name
Human Ortholog
Description Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication
Localization
Cell Percentages ER (20%), cytoplasm (7%), mixed (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Frt1

Frt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Frt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available