Standard name
Human Ortholog
Description Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.26 0.21 0.1 0.07 0 0.05 0 0 – 0 0 0 0.1 0.05 0 – – – 0.46 0.33 0.54 0.5 0.46 0.47
Bud 0.07 0.07 0.09 0.08 0.05 0.08 0.08 0.07 – 0.11 0.07 0.11 0 0 0 – – – 0.06 0.06 0 0.06 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0.12 0.11 0.08 0.12 0.06 0.06
Bud Site 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0.08 0 0 0.05 0 0 0 0 – 0 0 0 0.11 0.11 0.05 – – – 0 0 0 0.05 0.06 0.06
Cytoplasm 0.64 0.63 0.59 0.68 0.62 0.48 0.41 0.38 – 0.38 0.4 0.32 0.82 0.91 0.93 – – – 0.17 0.22 0.09 0.11 0.18 0.17
Endoplasmic Reticulum 0.05 0.08 0 0.07 0.05 0.07 0 0.05 – 0 0 0.06 0 0.05 0 – – – 0 0 0 0 0 0
Endosome 0 0 0.05 0 0.08 0 0 0 – 0 0 0 0 0 0 – – – 0 0.05 0.05 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.15 0 0.08 0.16 0.49 0.54 0.53 – 0.63 0.6 0.68 0 0 0 – – – 0.06 0.11 0.08 0.08 0.11 0.06
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0.05 0.06 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0.19 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 83 41 51 22 13 34 12 22 – 17 19 31 9 8 6 – – – 190 74 354 119 127 134
Bud 21 13 46 28 22 49 62 54 – 76 40 79 2 3 1 – – – 24 13 27 15 11 13
Bud Neck 4 2 9 1 0 1 0 0 – 0 1 0 0 1 0 – – – 50 25 51 28 16 16
Bud Site 15 7 18 2 5 18 16 14 – 16 14 9 1 0 1 – – – – – – – – –
Cell Periphery 25 6 22 18 8 9 14 18 – 16 22 30 10 19 9 – – – 17 6 27 11 15 16
Cytoplasm 207 121 311 226 264 310 312 289 – 262 226 230 76 156 155 – – – 67 49 61 26 48 49
Endoplasmic Reticulum 15 15 15 23 20 47 32 39 – 21 13 40 1 8 7 – – – 8 3 13 5 6 7
Endosome 0 0 27 11 35 4 23 19 – 8 3 15 1 0 0 – – – 11 10 31 4 6 14
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 4 1 1 0 2 2
Mitochondria 9 28 12 27 70 317 410 395 – 435 337 487 0 0 0 – – – 24 24 54 19 30 17
Nucleus 0 1 1 0 1 1 1 1 – 2 3 5 1 0 3 – – – 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 3 5 – 3 1 3 0 0 0 – – – 0 0 0 0 1 0
Nucleolus 0 0 1 0 0 2 2 9 – 0 1 4 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 3 2 32 18 24 20 34 16 – 29 7 22 0 0 0 – – – 0 2 10 1 1 3
SpindlePole 13 2 101 12 12 14 25 24 – 19 23 9 4 2 1 – – – 2 0 5 3 1 5
Vac/Vac Membrane 3 1 3 4 26 4 23 14 – 18 15 17 0 0 0 – – – 3 5 3 1 3 4
Unique Cell Count 323 191 526 333 426 646 756 752 688 560 721 93 172 167 410 223 656 239 276 288
Labelled Cell Count 399 239 649 392 500 831 969 919 922 725 981 105 197 183 410 223 656 239 276 288


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 4.6 4.7 4.5 4.5 3.8 3.9 4.2 – 3.7 3.6 3.9 6.5 6.7 7.2 – – – 6.4 7.2 7.0
Std Deviation (1e-4) 0.7 0.8 0.8 1.1 1.4 0.9 1.1 0.9 – 0.9 0.9 0.9 0.9 0.7 1.2 – – – 0.9 1.7 1.5
Intensity Change (Log2) – – – -0.07 -0.05 -0.31 -0.27 -0.16 – -0.33 -0.38 -0.25 0.48 0.51 0.62 – – – 0.44 0.62 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1167 2.2805 2.4116 2.2743 1.5543 2.0957 3.5431 3.916 3.9842 3.1137 3.7017 3.5737 4.183 3.7619 3.6984 4.1402 3.802 3.8244
Actin 0.16 0.0841 0.1219 0.0693 0.0673 0.1352 0.0986 0.0473 0.04 0.0334 0.0533 0.061 0.1202 0.0746 0.1439 0.0053 0.0402 0.095
Bud 0.0016 0.0027 0.0108 0.0053 0.004 0.0045 0.0011 0.0013 0.0029 0.003 0.0035 0.001 0.0027 0.0082 0.0144 0.008 0.002 0.0015
Bud Neck 0.0395 0.0113 0.0344 0.0579 0.0218 0.2262 0.0197 0.0097 0.0169 0.0145 0.004 0.285 0.0273 0.0146 0.0222 0.0004 0.0189 0.2723
Bud Periphery 0.0045 0.0048 0.0434 0.0065 0.0158 0.0138 0.0039 0.0023 0.0128 0.0281 0.0084 0.0033 0.0061 0.0112 0.0377 0.0212 0.0057 0.0056
Bud Site 0.0497 0.1614 0.0574 0.0063 0.0072 0.0075 0.0758 0.1944 0.0498 0.0031 0.0118 0.0039 0.0906 0.1933 0.0507 0.0017 0.0281 0.0043
Cell Periphery 0.0235 0.0067 0.0055 0.0012 0.0085 0.0054 0.0251 0.0083 0.016 0.0062 0.0066 0.0044 0.0168 0.0121 0.0078 0.0019 0.0022 0.0068
Cytoplasm 0.4346 0.4653 0.4297 0.6321 0.5189 0.4392 0.436 0.4031 0.4543 0.603 0.5297 0.4377 0.4372 0.5248 0.5106 0.6743 0.5492 0.4247
Cytoplasmic Foci 0.0369 0.0504 0.0287 0.0464 0.0201 0.0218 0.0174 0.0147 0.0118 0.0345 0.0323 0.0113 0.0256 0.0133 0.0183 0.0095 0.059 0.0158
Eisosomes 0.0031 0.0018 0.0009 0.0002 0.002 0.0009 0.0028 0.0007 0.0008 0.0008 0.0007 0.0005 0.0016 0.0009 0.0009 0.0002 0.0006 0.0007
Endoplasmic Reticulum 0.011 0.0097 0.0075 0.0083 0.0041 0.0035 0.006 0.0044 0.0057 0.0032 0.0048 0.002 0.0115 0.0026 0.0064 0.0038 0.0039 0.0017
Endosome 0.0096 0.0158 0.0246 0.0611 0.0098 0.0072 0.0244 0.0058 0.0047 0.0011 0.0274 0.0042 0.0218 0.0017 0.0075 0.0026 0.0226 0.0009
Golgi 0.0083 0.0108 0.0094 0.01 0.0019 0.0061 0.029 0.0029 0.0036 0.0011 0.0216 0.0027 0.017 0.0042 0.0078 0.0011 0.0069 0.0014
Lipid Particles 0.0074 0.0027 0.0013 0.0014 0.0009 0.0011 0.0034 0.0018 0.0021 0.0003 0.0057 0.0009 0.0135 0.0006 0.0036 0.0008 0.0063 0.0005
Mitochondria 0.0023 0.005 0.0035 0.0038 0.0048 0.0014 0.0027 0.0016 0.0072 0.0005 0.003 0.0005 0.0048 0.0011 0.0026 0.0006 0.0017 0.0003
None 0.1945 0.1542 0.2084 0.0735 0.3 0.1203 0.2279 0.297 0.3533 0.2626 0.2785 0.1788 0.1556 0.13 0.1553 0.2649 0.2171 0.1591
Nuclear Periphery 0.0033 0.0013 0.0014 0.0016 0.0006 0.0003 0.0026 0.0004 0.0007 0.0002 0.0012 0.0002 0.0116 0.0003 0.0011 0.0004 0.0051 0.0002
Nucleolus 0.0003 0.0004 0.0004 0.0002 0.002 0.0002 0.0002 0.0003 0.0002 0.0001 0.0003 0.0001 0.0005 0.0002 0.0002 0.0001 0.0005 0.0001
Nucleus 0.0016 0.0015 0.0017 0.0021 0.0025 0.001 0.0005 0.0007 0.0009 0.0004 0.0009 0.0005 0.0021 0.0009 0.0011 0.0005 0.0014 0.0004
Peroxisomes 0.0046 0.0053 0.0023 0.0066 0.0005 0.0018 0.0209 0.0015 0.014 0.0024 0.0023 0.001 0.0225 0.0038 0.0048 0.0004 0.0193 0.0076
Punctate Nuclear 0.0016 0.0013 0.0012 0.0011 0.0015 0.0007 0.0008 0.0004 0.0005 0.0004 0.0015 0.0004 0.0085 0.0003 0.0007 0.0003 0.0075 0.0005
Vacuole 0.0017 0.0031 0.0046 0.0041 0.005 0.0014 0.001 0.0012 0.0017 0.001 0.0021 0.0005 0.0015 0.0014 0.0021 0.0018 0.0013 0.0004
Vacuole Periphery 0.0003 0.0005 0.0011 0.0011 0.0009 0.0002 0.0003 0.0001 0.0002 0.0001 0.0005 0.0001 0.0011 0.0001 0.0003 0.0002 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.0097 9.4156 8.9431 20.534 14.2023 12.2112 21.5154 21.9749 18.4471 20.4725
Translational Efficiency 0.8565 1.2403 1.7502 0.5875 0.9109 1.6749 0.7122 0.7896 0.9842 0.8792

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
227 1454 542 1523 2088 1909 1483 1396 2315 3363 2025 2919

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 894.00 773.80 866.73 912.52 706.73 755.89 861.50 891.57 725.09 763.63 862.90 902.50
Standard Deviation 152.23 104.38 98.01 126.40 80.68 94.02 103.88 124.07 106.04 99.03 102.37 125.73
Intensity Change Log 2 — -0.208314 -0.044692 0.029581 — 0.097017 0.285692 0.335189 — -0.065491 0.110565 0.172541

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.045355 0.045671 0.019097 0.170301* 0.027868 0.017422 0.033305 0.124446* 0.029582 0.029636 0.029502 0.148371*
Bud Neck 0.018057 0.066626 0.050878 0.032822 0.133116 0.115923 0.083625 0.046066 0.121833 0.094609 0.074860 0.039156
Bud Site 0.146176* 0.091344 0.062375 0.212837* 0.055601 0.064913 0.145963* 0.190602* 0.064482 0.076341 0.123590* 0.202203*
Cell Periphery 0.004978 0.003339 0.002859 0.005860 0.004401 0.003321 0.004381 0.003815 0.004457 0.003329 0.003974 0.004882
Cytoplasm 0.235137* 0.343009* 0.363936* 0.252956* 0.405863* 0.355434* 0.406336* 0.301257* 0.389122* 0.350062* 0.394987* 0.276056*
Cytoplasmic Foci 0.103591 0.229497* 0.156848 0.041581 0.146635 0.201813* 0.050024 0.063234 0.142415 0.213782* 0.078616 0.051936
Eisosomes 0.003032 0.009416 0.001962 0.004702 0.020635 0.008303 0.001880 0.003880 0.018909 0.008784 0.001902 0.004309
Endoplasmic Reticulum 0.005601 0.003563 0.005349 0.005136 0.004833 0.002391 0.002482 0.002100 0.004908 0.002898 0.003249 0.003684
Endosome 0.030804 0.022593 0.016326 0.007766 0.013874 0.031501 0.003449 0.010465 0.015534 0.027650 0.006896 0.009057
Golgi 0.016378 0.036153 0.001944 0.004189 0.022997 0.025328 0.000694 0.004985 0.022348 0.030008 0.001028 0.004569
Lipid Particles 0.003273 0.015097 0.019236 0.003116 0.019880 0.017834 0.003572 0.003613 0.018251 0.016650 0.007765 0.003354
Mitochondria 0.007110 0.012508 0.000737 0.004892 0.022178 0.025003 0.000649 0.003377 0.020701 0.019600 0.000672 0.004167
Mitotic Spindle 0.006072 0.000720 0.001295 0.022558 0.000234 0.002375 0.001912 0.012254 0.000806 0.001659 0.001747 0.017630
None 0.000089 0.000891 0.001525 0.001169 0.000946 0.000930 0.000647 0.000261 0.000862 0.000913 0.000882 0.000735
Nuclear Periphery 0.000359 0.000076 0.000562 0.000190 0.000100 0.000119 0.000207 0.000122 0.000125 0.000100 0.000302 0.000158
Nuclear Periphery Foci 0.002008 0.000278 0.003005 0.001088 0.000126 0.000224 0.000808 0.000525 0.000310 0.000247 0.001396 0.000819
Nucleolus 0.000084 0.000207 0.000514 0.000122 0.000210 0.000546 0.000130 0.000081 0.000198 0.000399 0.000232 0.000102
Nucleus 0.034264 0.026115 0.125222 0.030314 0.049081 0.026631 0.097045 0.029825 0.047628 0.026408 0.104586 0.030080
Peroxisomes 0.024684 0.050964 0.021828 0.046955 0.036888 0.045845 0.015204 0.060421 0.035692 0.048058 0.016977 0.053395
Vacuole 0.307618* 0.040062 0.144174 0.147922 0.033104 0.051246 0.147575 0.137442 0.060022 0.046411 0.146665 0.142910
Vacuole Periphery 0.005329 0.001870 0.000330 0.003525 0.001432 0.002900 0.000114 0.001230 0.001814 0.002454 0.000172 0.002428

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.02 2.30 -10.87 -14.45 -18.51 4.82 -0.78 -14.54 -16.59 -13.70 -0.33 0.77 -22.29 -22.38 -22.44*
Bud Neck -11.77 -5.30 -4.07 10.52 3.33 2.92 9.77 18.17 15.81 7.25 5.56 10.38 20.07 19.46 9.23
Bud Site 2.29 4.26 -3.72 -12.89 -15.27 -2.50 -12.80 -17.57 -15.75 -4.73 -3.93 -9.95 -22.95 -20.73 -10.62
Cell Periphery 0.39 0.75 -0.51 -2.73 -3.89 2.90 1.03 2.57 -0.24 1.11 2.94 1.85 0.46 -2.46 -1.41
Cytoplasm -6.71 -6.46 -1.88 9.19 7.26 6.50 0.61 11.42 5.61 9.37 5.71 0.05 15.22 10.75 13.63
Cytoplasmic Foci -14.08 -5.80 5.99 32.38 15.33 -10.01 23.08 18.39 26.18 -3.81 -15.59 14.96 23.89 41.66 7.36
Eisosomes -5.98 0.60 -1.52 6.86 -4.07 10.73 18.35 16.20 8.02 -5.67 9.84 18.12 15.74 10.34 -7.30
Endoplasmic Reticulum 1.14 -0.28 0.23 -1.56 0.81 3.39 3.32 4.02 0.34 1.38 3.15 2.21 1.73 -1.47 -0.27
Endosome 0.73 2.17 4.30 9.87 5.32 -10.93 12.10 2.88 12.02 -5.30 -9.72 8.52 5.95 15.97 -1.92
Golgi -4.45 2.22 1.88 16.96 -2.00 -1.36 20.32 14.16 14.55 -5.06 -5.21 19.01 14.88 22.33 -5.49
Lipid Particles -10.26 -7.98 -0.11 10.62 8.05 1.41 15.29 14.84 12.22 -0.28 1.38 9.71 15.76 16.31 5.93
Mitochondria -2.27 1.69 0.51 6.18 -3.63 -1.29 14.88 12.46 13.24 -4.41 0.60 14.85 11.81 14.11 -5.35
Mitotic Spindle 2.22 1.89 -4.15 -7.04 -6.66 -3.78 -2.20 -5.27 -4.15 -4.34 -2.21 -1.50 -8.60 -8.01 -7.81
None -2.48 -3.08 -1.96 -0.20 0.80 0.03 0.85 2.02 2.73 2.49 -0.14 -0.00 0.58 0.90 0.79
Nuclear Periphery 2.90 -1.62 1.56 -4.58 5.45 -0.92 -6.96 -2.23 -1.26 4.10 1.38 -8.08 -2.48 -4.19 5.89
Nuclear Periphery Foci 2.89 -2.06 1.17 -3.62 4.59 -2.85 -9.31 -8.32 -5.61 2.85 1.02 -8.86 -4.01 -4.77 3.38
Nucleolus -0.96 -5.21 0.16 1.62 6.99 -1.86 5.01 6.91 2.41 2.61 -1.88 -1.94 4.23 2.60 5.52
Nucleus 2.36 -8.41 1.18 -3.88 13.46 11.71 -13.85 6.65 -3.31 17.53 12.18 -17.21 6.98 -5.12 22.05
Peroxisomes -5.58 -0.01 -4.06 1.91 -5.77 -3.44 11.13 -4.97 -2.48 -11.10 -5.79 10.28 -5.23 -0.51 -13.00
Vacuole 15.44 9.12 7.39 -24.85 -4.21 -7.93 -26.04 -23.03 -19.40 -0.17 5.56 -19.43 -23.16 -31.13 -3.15
Vacuole Periphery 1.15 1.93 0.63 -1.04 -2.86 -4.28 10.86 0.93 3.77 -2.70 -2.44 6.82 -0.52 0.89 -3.74
Standard name
Human Ortholog
Description Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
Localization
Cell Percentages actin (15%), cytoplasm (12%), mixed (34%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Scd5

Scd5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Scd5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available