Standard name
Human Ortholog
Description Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.65 0.65 0.79 0.89 0.97 0.95 0.93 0.9 0.89 0.9 0.76 0.8 0.87 0.97 0.98 0.72 0.7 0.71 0.39 0.45 0.45 0.59 0.79 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.14 0.22 0.2 0 0.13 0.21 0.21 0.3 0.25 0.41 0.36 0.09 0 0 0 0 0 0.07 0 0.11 0.05 0.07 0.05
Nucleus 0.22 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.06 0.22 0.19 0.1 0.12 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.24 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0.21 0.3 0.21 0.19 0.21 0.18 0.15 0.07 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 3 0 0 4
Bud 0 0 7 0 0 0 4 4 2 0 0 3 0 0 1 0 0 0 3 2 4 0 0 6
Bud Neck 0 0 7 0 4 6 1 8 1 1 1 1 0 1 0 0 0 1 0 1 1 0 0 0
Bud Site 0 0 0 0 0 0 3 3 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 3 0 1 0 3 0 1 1 1 1 0 0 0 1 0 0 0
Cytoplasm 89 132 199 185 281 325 339 400 150 129 172 228 86 283 313 234 106 164 66 107 120 46 142 117
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 1 0 0 0 0 1 0 17 3 18 1 0 0 0 0 1
Endosome 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 13 9 4 3 2 2 0 0 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10 1 3 1 1 1 0 0 0
Mitochondria 1 28 56 42 12 43 78 92 50 36 94 104 9 1 11 13 2 7 11 10 29 3 11 7
Nucleus 30 20 5 2 1 0 2 6 0 1 3 0 3 1 0 19 8 14 36 43 25 9 0 0
Nuclear Periphery 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0
SpindlePole 1 0 1 1 3 3 1 4 6 1 3 4 0 0 0 1 1 1 4 10 11 0 2 3
Vac/Vac Membrane 24 48 5 0 1 1 1 1 0 0 0 0 10 9 3 67 45 49 31 49 46 11 11 7
Unique Cell Count 136 203 251 207 289 341 366 443 169 144 227 285 99 291 320 324 152 231 170 237 267 79 179 162
Labelled Cell Count 146 231 281 230 302 379 430 522 210 169 273 343 108 297 329 377 177 261 170 237 267 79 179 162


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 3.7 3.0 2.7 3.1 2.8 2.7 2.6 2.4 2.2 2.4 2.3 4.0 4.2 3.6 5.5 6.2 5.9 3.7 4.3 5.4
Std Deviation (1e-4) 1.0 0.8 0.8 0.7 1.3 1.3 1.2 1.1 1.7 0.9 1.2 1.4 1.4 1.3 1.1 1.5 2.4 2.1 0.6 1.2 2.6
Intensity Change (Log2) – – – -0.15 0.05 -0.1 -0.16 -0.2 -0.32 -0.42 -0.3 -0.4 0.42 0.49 0.26 0.89 1.06 0.99 0.3 0.51 0.85


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 6.7 8.1 6.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.233 0.2572 0.2723 0.2398 -0.1186 0.186 2.4678 2.1688 2.2067 1.8513 1.8588 2.2397 2.0064 2.577 2.6599 2.6242 2.7665 2.6734
Actin 0.029 0.0079 0.0126 0.0216 0.0021 0.002 0.031 0.0002 0.0203 0.0001 0.0029 0.0008 0.0013 0.0003 0.0132 0.0245 0.007 0.0015
Bud 0.001 0.0014 0.0003 0.0005 0.0007 0.003 0.0008 0.0001 0.0015 0.0001 0.0014 0.0003 0.0003 0.0002 0.0005 0.0017 0.0003 0.0003
Bud Neck 0.0148 0.0004 0.0014 0.0008 0.0028 0.0063 0.0013 0.0003 0.001 0.0003 0.0006 0.0076 0.0004 0.0004 0.0008 0.0018 0.0009 0.0043
Bud Periphery 0.0019 0.0042 0.0006 0.0029 0.0008 0.0031 0.0015 0.0001 0.0048 0 0.003 0.0003 0.0004 0.0002 0.0009 0.0051 0.0002 0.0004
Bud Site 0.009 0.0025 0.003 0.0006 0.0035 0.0069 0.0054 0.0016 0.0067 0.0001 0.0016 0.0004 0.0007 0.0058 0.0021 0.0057 0.0003 0.0004
Cell Periphery 0.0004 0.0002 0.0001 0.0002 0.0001 0.0002 0.0004 0.0001 0.0012 0 0.0001 0 0.0001 0.0003 0.0001 0.0008 0 0.0001
Cytoplasm 0.1807 0.2244 0.1859 0.2223 0.313 0.2225 0.094 0.154 0.1428 0.0999 0.1341 0.1732 0.2023 0.2022 0.1695 0.1032 0.164 0.1964
Cytoplasmic Foci 0.026 0.0079 0.0099 0.0064 0.0343 0.0132 0.0196 0.0056 0.0132 0.0053 0.0266 0.008 0.0097 0.0072 0.008 0.0019 0.0129 0.014
Eisosomes 0.0006 0.0001 0.0002 0.0004 0.0001 0.0001 0.0004 0.0001 0.0006 0 0.0001 0.0001 0.0001 0.0001 0.0003 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0094 0.0049 0.0036 0.0022 0.0059 0.0043 0.0088 0.0022 0.0033 0.0007 0.0028 0.0015 0.0059 0.0049 0.0046 0.0014 0.0026 0.0017
Endosome 0.0215 0.0038 0.0058 0.0041 0.0106 0.007 0.022 0.0027 0.0152 0.0007 0.0089 0.0022 0.0074 0.0044 0.0063 0.005 0.0122 0.0065
Golgi 0.0069 0.0014 0.003 0.0016 0.0018 0.0007 0.0051 0.0001 0.0088 0 0.001 0.0002 0.0009 0.0004 0.0015 0.0043 0.011 0.0004
Lipid Particles 0.0176 0.0025 0.0019 0.0036 0.0066 0.0015 0.0136 0.0004 0.0048 0.0001 0.0098 0.0003 0.0046 0.0003 0.0034 0.0012 0.0035 0.0008
Mitochondria 0.0056 0.0016 0.0149 0.0496 0.0036 0.0091 0.0044 0.0006 0.0072 0.0004 0.0043 0.0006 0.001 0.0011 0.0037 0.0043 0.0039 0.0008
None 0.4906 0.5156 0.5349 0.3523 0.3046 0.3673 0.6967 0.7005 0.6363 0.664 0.5692 0.591 0.6927 0.5238 0.5677 0.4803 0.5813 0.5815
Nuclear Periphery 0.0302 0.0067 0.0131 0.0081 0.0126 0.0077 0.0175 0.0037 0.0066 0.0017 0.0184 0.0073 0.0083 0.0064 0.0151 0.0035 0.0057 0.0032
Nucleolus 0.0057 0.0022 0.0028 0.0054 0.0033 0.004 0.0032 0.0027 0.0018 0.0008 0.0035 0.0024 0.0008 0.0018 0.0018 0.0012 0.0016 0.0026
Nucleus 0.1137 0.1988 0.1752 0.2469 0.2636 0.3121 0.0442 0.113 0.0966 0.2206 0.1723 0.1659 0.0479 0.2303 0.1708 0.3468 0.1508 0.1644
Peroxisomes 0.0115 0.0003 0.0021 0.0515 0.0069 0.0009 0.0086 0.0002 0.0066 0.0002 0.0011 0.0009 0.0032 0.0002 0.0031 0.0005 0.0062 0.0008
Punctate Nuclear 0.0165 0.0097 0.026 0.0163 0.0185 0.0255 0.0163 0.0097 0.0176 0.0044 0.0338 0.0353 0.0068 0.0072 0.0242 0.0045 0.0319 0.0181
Vacuole 0.0057 0.0028 0.0022 0.0017 0.004 0.0025 0.0036 0.002 0.0023 0.0004 0.0028 0.0011 0.0045 0.0025 0.0017 0.0016 0.0031 0.0016
Vacuole Periphery 0.0015 0.0003 0.0005 0.001 0.0006 0.0004 0.0015 0.0002 0.0008 0.0001 0.0018 0.0007 0.0008 0.0003 0.0006 0.0005 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.8693 13.005 15.094 17.5272 10.6401 11.5573 17.5324 16.4982 13.6603 13.1088
Translational Efficiency 0.3339 0.5339 0.4642 0.3511 0.3145 0.2974 0.3659 0.3247 0.4055 0.2848

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1370 446 1417 1190 1120 1495 657 247 2490 1941 2074 1437

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 592.37 723.25 867.44 770.42 631.40 674.12 854.42 816.86 609.93 685.41 863.32 778.40
Standard Deviation 93.16 92.85 97.42 105.45 84.49 119.12 89.48 99.95 91.45 115.49 95.17 105.98
Intensity Change Log 2 — 0.287996 0.550265 0.379147 — 0.094451 0.436391 0.371535 — 0.191382 0.492635 0.375224

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000071 0.000597 0.000243 0.000864 0.000048 0.000125 0.000215 0.002324 0.000061 0.000234 0.000234 0.001115
Bud Neck 0.002648 0.034440 0.005677 0.012304 0.009917 0.024555 0.002232 0.009645 0.005917 0.026826 0.004586 0.011847
Bud Site 0.003595 0.010380 0.009855 0.028283 0.002928 0.008811 0.001644 0.028484 0.003295 0.009172 0.007254 0.028317
Cell Periphery 0.000235 0.000193 0.000100 0.000290 0.000198 0.000451 0.000122 0.000580 0.000218 0.000392 0.000107 0.000340
Cytoplasm 0.534192* 0.253678* 0.144911* 0.276330* 0.399115* 0.328788* 0.111770* 0.227562* 0.473434* 0.311529* 0.134412* 0.267947*
Cytoplasmic Foci 0.061885 0.260487* 0.002153 0.018432 0.168326 0.154616 0.005345 0.019349 0.109762 0.178943 0.003164 0.018589
Eisosomes 0.000064 0.000184 0.000020 0.000050 0.000128 0.000803 0.000047 0.000080 0.000093 0.000660 0.000029 0.000055
Endoplasmic Reticulum 0.000613 0.001135 0.003550 0.003543 0.000555 0.001226 0.003235 0.003913 0.000587 0.001205 0.003450 0.003606
Endosome 0.001437 0.024944 0.000722 0.012985 0.005878 0.016978 0.002020 0.021973 0.003435 0.018808 0.001133 0.014530
Golgi 0.000265 0.006668 0.000046 0.004914 0.000969 0.002635 0.000165 0.008566 0.000582 0.003562 0.000084 0.005542
Lipid Particles 0.002431 0.010665 0.000555 0.002801 0.014089 0.015440 0.001439 0.001505 0.007675 0.014343 0.000835 0.002578
Mitochondria 0.001151 0.027502 0.000741 0.009678 0.004738 0.012220 0.001200 0.008903 0.002764 0.015731 0.000886 0.009545
Mitotic Spindle 0.000108 0.011632 0.002201 0.041773 0.001369 0.003091 0.002330 0.032366 0.000675 0.005053 0.002242 0.040156
None 0.052312 0.004631 0.004371 0.017334 0.012193 0.022609 0.006078 0.008057 0.034267 0.018478 0.004912 0.015740
Nuclear Periphery 0.000335 0.000652 0.001431 0.002212 0.000866 0.001409 0.001593 0.001934 0.000574 0.001235 0.001482 0.002164
Nuclear Periphery Foci 0.000567 0.000375 0.000460 0.000969 0.000314 0.000650 0.000631 0.001042 0.000453 0.000587 0.000514 0.000982
Nucleolus 0.001421 0.004403 0.000448 0.001830 0.002314 0.004579 0.001002 0.001922 0.001823 0.004539 0.000624 0.001846
Nucleus 0.322856* 0.253883* 0.806171* 0.501367* 0.347737* 0.348213* 0.835329* 0.510281* 0.334048* 0.326538* 0.815408* 0.502899*
Peroxisomes 0.000671 0.035119 0.000110 0.002689 0.005084 0.010311 0.000272 0.005529 0.002656 0.016011 0.000162 0.003177
Vacuole 0.012838 0.055977 0.015996 0.057722 0.022541 0.040221 0.023084 0.103247 0.017202 0.043841 0.018241 0.065547
Vacuole Periphery 0.000305 0.002458 0.000238 0.003631 0.000695 0.002269 0.000247 0.002738 0.000480 0.002312 0.000241 0.003477

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.68 -4.79 -5.35 -0.04 -3.23 -4.73 -6.70 -2.24 -2.05 -1.67 -3.32 -7.89 -5.70 -3.89 -3.51
Bud Neck -7.70 -3.41 -7.65 5.41 -3.72 -6.39 5.01 1.31 8.10 -5.05 -11.34 0.87 -4.36 8.32 -5.11
Bud Site -4.03 -2.61 -4.87 -2.16 -2.35 -3.49 0.83 -2.27 -0.83 -2.55 -4.46 -2.42 -5.56 -2.66 -3.58
Cell Periphery 1.09 3.41 1.08 0.09 -3.21 -5.07 3.13 -0.48 1.89 -1.18 -4.12 4.58 0.60 4.36 -3.11
Cytoplasm 20.97 48.05 31.72 2.17 -9.92 6.92 28.70 15.21 10.96 -3.85 20.66 52.99 31.66 11.44 -11.32
Cytoplasmic Foci -17.18 22.40 17.11 22.07 -8.28 1.69 29.28 26.91 27.00 -3.29 -12.28 34.66 30.15 34.48 -8.45
Eisosomes -6.65 9.28 0.08 6.75 -13.14 -3.52 9.61 3.16 3.69 -3.84 -3.85 14.69 5.68 4.02 -10.82
Endoplasmic Reticulum -2.36 -15.75 -13.43 -10.39 -0.62 -3.88 -14.57 -10.18 -9.06 -4.22 -4.24 -20.92 -16.70 -13.53 -2.26
Endosome -9.51 2.99 -5.24 6.87 -5.94 -7.15 4.96 -0.84 3.04 -2.35 -12.12 6.56 -3.55 7.74 -6.10
Golgi -4.55 7.79 -2.81 3.02 -3.17 -4.26 3.89 -0.74 0.20 -1.24 -7.04 5.31 -2.43 2.10 -3.34
Lipid Particles -7.12 8.87 0.66 6.93 -2.86 -0.83 9.64 9.39 11.58 -1.11 -6.11 11.11 7.84 12.22 -2.59
Mitochondria -6.31 1.56 -2.58 5.18 -2.85 -4.59 3.79 1.10 4.72 -1.24 -8.74 4.25 -1.28 6.24 -3.02
Mitotic Spindle -3.51 -2.65 -8.36 -4.02 -7.56 -1.87 -0.51 -3.01 -2.67 -2.89 -4.65 -2.14 -8.71 -7.11 -8.12
None 12.57 12.99 10.47 -4.27 -5.37 -3.17 3.47 1.97 5.03 -1.94 5.04 13.19 9.47 3.39 -5.46
Nuclear Periphery -3.17 -13.99 -18.05 -16.55 -10.79 -2.86 -9.20 -10.55 -7.12 -5.45 -4.66 -16.17 -19.69 -12.19 -11.59
Nuclear Periphery Foci 2.77 -1.33 -4.96 -6.42 -4.05 -3.23 -3.03 -4.63 -3.38 -2.95 -1.51 -3.48 -7.10 -5.48 -4.82
Nucleolus -3.68 9.34 -2.03 2.98 -5.97 -5.10 5.35 3.24 7.55 -1.18 -7.48 9.77 -0.08 6.70 -5.69
Nucleus 4.73 -53.07 -19.83 -18.76 21.77 0.11 -40.46 -9.85 -9.93 11.12 1.06 -66.69 -22.14 -21.27 24.89
Peroxisomes -9.54 2.85 -1.31 9.38 -3.64 -4.49 5.91 3.02 7.55 -1.82 -11.64 6.53 2.81 13.18 -4.00
Vacuole -8.84 -5.79 -17.35 -6.71 -14.50 -6.81 -4.95 -9.58 -8.44 -8.18 -12.44 -6.91 -19.20 -13.51 -16.08
Vacuole Periphery -7.46 0.56 -2.69 1.55 -2.82 -3.97 2.85 -0.34 2.79 -1.68 -6.11 2.58 -2.41 1.76 -3.07
Standard name
Human Ortholog
Description Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress
Localization
Cell Percentages cytoplasm (26%), nucleus (4%), mixed (51%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00016)
Subcompartmental Group N/A

Rev1

Rev1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rev1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available