Standard name
Human Ortholog
Description Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.96 0.98 0.95 0.84 0.79 0.72 0.68 0.64 0.64 1.0 1.0 0.99 0.97 0.97 0.95 0.96 0.95 0.96 0.97 0.97 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.23 0.21 0.36 0.38 0.47 0.43 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2
Bud 2 2 1 0 1 0 1 2 3 1 0 3 1 1 0 0 0 0 0 1 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 7 2 0 5 3 13 8 8 8 12 10 8 4 3 2 5 0 0 0 0 0 0 0
Cytoplasm 218 289 216 131 241 223 322 245 191 208 156 206 249 444 354 142 114 19 217 274 201 125 226 197
Endoplasmic Reticulum 0 1 0 0 1 2 5 3 5 6 8 10 0 0 3 7 5 0 0 0 0 0 0 2
Endosome 0 0 0 1 1 0 2 1 0 1 0 0 0 0 0 1 0 0 2 1 0 0 1 1
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 2
Mitochondria 5 0 2 6 3 25 87 64 96 117 114 138 0 2 0 6 2 1 0 2 0 0 0 2
Nucleus 0 0 1 0 0 1 1 2 1 1 0 0 1 1 1 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 1 2 3 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 0 2 1 0 9 19 8 17 7 15 0 0 1 4 0 0 0 3 4 0 0 1
Unique Cell Count 221 292 218 136 245 234 384 309 266 306 244 321 249 446 359 146 117 20 226 288 210 130 232 217
Labelled Cell Count 225 302 222 141 253 254 441 346 315 362 299 384 259 453 362 162 126 20 226 288 210 130 232 217


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 7.5 7.5 7.2 7.6 6.7 6.2 6.7 6.0 6.0 5.9 5.9 8.2 8.6 8.7 5.4 5.8 7.5 6.5 7.3 9.0
Std Deviation (1e-4) 0.8 1.2 1.4 1.4 1.2 1.1 1.6 1.4 1.2 1.4 1.4 1.3 1.5 1.3 1.3 1.4 1.4 1.5 1.2 1.5 1.8
Intensity Change (Log2) – – – -0.06 0.01 -0.16 -0.28 -0.18 -0.32 -0.32 -0.34 -0.35 0.12 0.2 0.21 -0.47 -0.36 -0.01 -0.21 -0.03 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.9 0 0 0 2.5 0 0 0 0 0 0 0
Cytoplasm -1.8 -0.7 -2.4 -5.8 -6.8 -8.2 -8.9 -9.5 -9.6 1.5 0.7 -0.5 -1.3 -1.2 -1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.4 7.2 6.8 9.6 10.1 11.3 10.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.7 0 3.5 0 3.2 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1638 5.316 5.0212 4.3743 4.6322 4.9094 4.7484 5.6603 5.5041 4.226 4.9821 5.4758 4.9979 6.3117 5.4445 5.719 5.4159 5.7291
Actin 0.0155 0.0003 0.014 0.0004 0.0003 0.0018 0.027 0.0004 0.0134 0.0129 0.0139 0.0009 0.0063 0.0012 0.0104 0.0004 0.0002 0.0007
Bud 0.0002 0.0002 0.0006 0.0005 0.0003 0.0001 0.0001 0.0001 0.0003 0.0002 0.0001 0.0001 0.0017 0.0004 0.0004 0.0002 0.0001 0.0001
Bud Neck 0.002 0.0002 0.0009 0.0005 0.0007 0.0013 0.0006 0.0001 0.0008 0.0004 0.0003 0.0005 0.0024 0.0094 0.0117 0.0003 0.0003 0.0006
Bud Periphery 0.0003 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0 0.0003 0.0001 0.0001 0 0.0016 0.0003 0.0005 0.0001 0 0.0001
Bud Site 0.0043 0.0006 0.0061 0.0004 0.0003 0.0001 0.0007 0.0012 0.0019 0.0005 0.0008 0 0.0079 0.0099 0.0032 0.0002 0 0.0001
Cell Periphery 0.0007 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0 0 0.0003 0.0003 0.0002 0 0 0
Cytoplasm 0.8435 0.9828 0.9017 0.9798 0.9826 0.9559 0.8411 0.9743 0.887 0.9107 0.8461 0.9415 0.7399 0.9473 0.8423 0.9259 0.9902 0.953
Cytoplasmic Foci 0.0143 0.0022 0.0141 0.003 0.0033 0.0059 0.0156 0.0043 0.0088 0.0173 0.0237 0.0054 0.051 0.0107 0.0073 0.0078 0.0025 0.0052
Eisosomes 0.0004 0 0.0002 0 0 0 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0101 0.0019 0.0033 0.0024 0.0025 0.0075 0.0078 0.0025 0.003 0.0019 0.0034 0.0044 0.0047 0.0013 0.0039 0.002 0.0006 0.0016
Endosome 0.019 0.0022 0.0054 0.0026 0.0043 0.0047 0.0087 0.0033 0.0077 0.0046 0.0151 0.0046 0.0537 0.0023 0.0146 0.0039 0.0005 0.0093
Golgi 0.0037 0.0001 0.0017 0.0002 0.0003 0.0011 0.0023 0.0003 0.006 0.0051 0.0063 0.0007 0.0147 0.0024 0.01 0.0004 0 0.0029
Lipid Particles 0.0086 0 0.003 0.0001 0 0.004 0.0022 0.0001 0.008 0.0041 0.0129 0 0.0146 0.0005 0.0031 0.0001 0 0.0008
Mitochondria 0.0042 0.0001 0.0011 0.0001 0.0001 0.0005 0.0009 0.0001 0.0058 0.0009 0.0016 0.0008 0.0042 0.0004 0.0187 0.0003 0 0.0046
None 0.0349 0.0062 0.0309 0.0048 0.0015 0.0101 0.0756 0.0108 0.0375 0.008 0.0561 0.0388 0.0706 0.0098 0.0587 0.0555 0.0041 0.019
Nuclear Periphery 0.014 0.0004 0.0016 0.0008 0.0005 0.0014 0.0025 0.0004 0.001 0.0004 0.0008 0.0003 0.0033 0.0002 0.0013 0.0005 0.0002 0.0002
Nucleolus 0.0008 0 0.0003 0 0 0 0.0001 0 0.0002 0 0.0001 0 0.0005 0.0001 0.001 0 0 0
Nucleus 0.005 0.0009 0.0024 0.0016 0.0013 0.0018 0.0025 0.0007 0.001 0.0008 0.0008 0.0009 0.0032 0.0006 0.0013 0.0012 0.0007 0.0006
Peroxisomes 0.0048 0 0.0078 0 0 0.0018 0.0056 0 0.0076 0.0307 0.0154 0.0001 0.0039 0.0022 0.009 0.0001 0 0.0002
Punctate Nuclear 0.009 0.0001 0.0027 0.0001 0.0001 0.0003 0.0042 0.0002 0.0077 0.0003 0.0009 0.0002 0.0108 0.0001 0.0007 0.0003 0.0001 0.0001
Vacuole 0.0034 0.0014 0.0013 0.0021 0.0014 0.0013 0.0015 0.0007 0.0012 0.0007 0.0012 0.0004 0.0035 0.0005 0.001 0.0008 0.0002 0.0005
Vacuole Periphery 0.0012 0.0001 0.0002 0.0003 0.0002 0.0003 0.0003 0.0001 0.0005 0.0001 0.0003 0.0001 0.0009 0 0.0006 0.0002 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.1362 7.7821 10.1056 21.1167 18.8115 26.8267 15.7369 24.7831 41.0043 28.4635
Translational Efficiency 0.8807 0.8908 1.0643 1.1743 0.6571 1.1687 1.012 0.8777 0.931 0.9245

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1764 411 1880 1585 1168 459 321 1605 2932 870 2201 3190

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 844.57 896.17 1139.37 1089.26 823.87 921.97 1304.60 1078.10 836.32 909.78 1163.47 1083.65
Standard Deviation 98.60 144.79 141.18 164.03 96.36 132.21 146.92 157.23 98.24 138.89 153.54 160.74
Intensity Change Log 2 — 0.085555 0.431947 0.367059 — 0.162303 0.663119 0.388002 — 0.123963 0.550727 0.377439

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000089 0.000333 0.000098 0.002098 0.000140 0.000416 0.000332 0.000482 0.000109 0.000377 0.000133 0.001285
Bud Neck 0.008649 0.004934 0.001636 0.006775 0.003539 0.007706 0.001048 0.004511 0.006613 0.006396 0.001550 0.005636
Bud Site 0.008940 0.022787 0.006414 0.035665 0.006146 0.024181 0.008813 0.030875 0.007827 0.023523 0.006764 0.033255
Cell Periphery 0.000120 0.000100 0.000048 0.000151 0.000123 0.000122 0.000112 0.000090 0.000121 0.000111 0.000057 0.000120
Cytoplasm 0.743104* 0.634230* 0.773582* 0.582596* 0.684726* 0.585674* 0.716501* 0.660711* 0.719848* 0.608612* 0.765257* 0.621898*
Cytoplasmic Foci 0.069423 0.175279 0.023144 0.035346 0.162731 0.211227* 0.030152 0.032404 0.106594 0.194245* 0.024166 0.033866
Eisosomes 0.000041 0.000038 0.000009 0.000021 0.000063 0.000064 0.000018 0.000016 0.000050 0.000051 0.000011 0.000018
Endoplasmic Reticulum 0.000345 0.000552 0.001049 0.001459 0.001157 0.001159 0.001202 0.000742 0.000669 0.000872 0.001072 0.001099
Endosome 0.002783 0.027445 0.002677 0.014866 0.008888 0.024441 0.004318 0.013739 0.005215 0.025860 0.002916 0.014299
Golgi 0.002022 0.004429 0.000156 0.014890 0.002202 0.003610 0.000285 0.006285 0.002094 0.003997 0.000174 0.010561
Lipid Particles 0.001358 0.001463 0.000700 0.001256 0.002356 0.004248 0.001456 0.000488 0.001756 0.002932 0.000810 0.000869
Mitochondria 0.001388 0.002322 0.000115 0.004329 0.001007 0.001816 0.000067 0.009184 0.001236 0.002055 0.000108 0.006772
Mitotic Spindle 0.000398 0.005326 0.004855 0.118691* 0.002295 0.002190 0.001550 0.058448 0.001154 0.003671 0.004373 0.088381
None 0.015133 0.001762 0.003271 0.003205 0.003605 0.000799 0.002830 0.003486 0.010540 0.001254 0.003207 0.003346
Nuclear Periphery 0.000072 0.000072 0.000468 0.000841 0.000235 0.000108 0.000358 0.000627 0.000137 0.000091 0.000452 0.000733
Nuclear Periphery Foci 0.000277 0.001940 0.001894 0.002806 0.001047 0.002126 0.010437 0.001958 0.000584 0.002038 0.003140 0.002379
Nucleolus 0.000709 0.000321 0.000206 0.000634 0.000373 0.000563 0.000083 0.000239 0.000575 0.000448 0.000188 0.000435
Nucleus 0.090377 0.017728 0.068737 0.039768 0.056831 0.016491 0.053653 0.036693 0.077014 0.017076 0.066537 0.038221
Peroxisomes 0.001231 0.004286 0.000147 0.002926 0.000886 0.003845 0.000155 0.002261 0.001094 0.004053 0.000148 0.002591
Vacuole 0.052784 0.092972 0.110460 0.123850 0.060487 0.106892 0.166427 0.128272 0.055853 0.100316 0.118623 0.126075
Vacuole Periphery 0.000756 0.001681 0.000333 0.007827 0.001161 0.002324 0.000204 0.008490 0.000917 0.002020 0.000314 0.008160

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.04 -2.80 -2.97 -1.66 -2.82 -4.43 -5.75 -2.97 1.94 2.01 -6.93 -3.13 -3.66 -0.96 -3.20
Bud Neck 3.97 8.74 5.46 1.13 -3.71 -3.71 6.67 1.64 4.40 -3.70 0.48 9.37 6.01 4.24 -3.98
Bud Site -5.73 2.59 -5.10 1.87 -6.79 -7.55 -1.60 -5.00 3.50 -3.17 -9.19 1.61 -6.84 3.82 -7.56
Cell Periphery 1.65 12.75 1.89 0.17 -4.03 0.21 0.92 9.25 6.01 7.05 1.25 13.25 6.37 3.54 -2.69
Cytoplasm 7.71 1.66 24.19 6.12 20.82 7.06 4.21 8.69 -1.13 0.89 11.25 0.80 23.70 3.49 19.48
Cytoplasmic Foci -12.02 18.22 14.29 16.99 -3.06 -5.03 25.77 27.92 21.55 0.67 -13.40 31.18 29.32 27.24 -2.88
Eisosomes 0.76 15.84 11.10 5.34 -7.58 -0.07 11.89 14.06 7.96 3.04 -0.41 21.24 17.92 9.49 -7.70
Endoplasmic Reticulum -2.41 -3.11 -1.24 -0.19 1.15 0.02 0.25 2.62 1.35 3.86 -0.73 -1.49 0.82 1.16 2.13
Endosome -6.87 0.98 -3.08 6.18 -3.58 -6.44 6.85 3.80 7.79 -1.45 -9.79 8.08 0.18 9.65 -4.63
Golgi -1.94 14.61 -1.42 0.61 -3.43 -1.97 5.48 0.45 2.08 -3.16 -2.78 12.01 -0.97 1.52 -4.61
Lipid Particles -0.34 2.67 -0.26 0.19 -5.54 -1.02 2.89 8.93 1.88 5.12 -1.18 5.33 4.50 1.88 -2.12
Mitochondria -0.50 6.81 0.49 0.63 -2.12 -0.69 2.32 -1.71 -0.72 -2.85 -0.77 5.70 -1.27 0.02 -3.45
Mitotic Spindle -1.75 -6.00 -12.84 -10.00 -11.10 0.10 -0.02 -7.92 -7.92 -8.22 -1.65 -4.94 -15.16 -12.75 -12.79
None 11.07 11.63 12.31 -1.31 0.15 4.55 2.32 1.68 -3.99 -1.14 12.49 10.97 12.01 -3.43 -0.10
Nuclear Periphery -0.45 -9.63 -14.31 -14.19 -4.43 1.25 -3.21 -3.79 -9.60 -1.13 1.07 -7.37 -11.66 -16.80 -3.58
Nuclear Periphery Foci -3.80 -9.95 -10.30 -0.20 -0.54 -1.58 -9.03 -3.73 0.66 8.29 -3.54 -11.92 -10.95 0.42 5.02
Nucleolus 8.71 16.66 3.31 -0.84 -2.00 -0.69 8.82 4.13 1.16 -5.90 0.68 16.45 5.27 0.87 -2.38
Nucleus 30.27 5.11 10.10 -17.93 4.84 16.22 -0.69 0.96 -16.96 1.38 34.07 1.49 9.47 -24.69 6.79
Peroxisomes -3.30 5.72 -0.39 3.14 -5.14 -6.65 10.14 -0.42 5.11 -2.64 -5.93 8.04 -0.40 5.44 -5.12
Vacuole -6.92 -17.81 -31.48 -19.38 -15.52 -7.47 -13.58 -27.99 -16.32 0.59 -10.48 -21.33 -42.44 -25.16 -15.44
Vacuole Periphery -3.67 6.79 -2.28 -0.33 -3.25 -1.25 2.74 -1.95 -0.29 -3.81 -2.27 4.14 -3.06 -0.37 -4.86
Standard name
Human Ortholog
Description Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pyk2

Pyk2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pyk2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available