Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in splicing Group I aI5-beta intron from COX1 mRNA; mitochondrial matrix protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 8 | 8 | 8 | 6 | 1 | 3 | – | 1 | 4 | 0 | 1 | 9 | 20 | 2 | 35 | 23 | 14 | 2 | 4 | 4 | 0 | 5 | 7 |
Bud | 5 | 2 | 0 | 4 | 4 | 7 | 8 | – | 6 | 2 | 5 | 11 | 0 | 5 | 4 | 3 | 6 | 5 | 0 | 2 | 1 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 1 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 4 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 3 | 2 | – | 3 | 2 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 3 | 18 | 3 | 2 | 4 | 3 | 5 | – | 5 | 2 | 2 | 5 | 8 | 7 | 11 | 41 | 30 | 15 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 8 | 3 | 11 | 14 | 14 | 15 | – | 7 | 7 | 15 | 7 | 23 | 30 | 38 | 93 | 79 | 79 | 0 | 1 | 0 | 1 | 3 | 7 |
Endoplasmic Reticulum | 1 | 18 | 0 | 1 | 6 | 5 | 7 | – | 1 | 3 | 3 | 5 | 3 | 12 | 6 | 11 | 8 | 16 | 1 | 4 | 0 | 0 | 2 | 1 |
Endosome | 11 | 6 | 13 | 5 | 39 | 20 | 44 | – | 20 | 3 | 2 | 9 | 17 | 27 | 38 | 44 | 30 | 37 | 14 | 26 | 11 | 11 | 15 | 19 |
Golgi | 4 | 57 | 9 | 14 | 16 | 3 | 8 | – | 3 | 2 | 0 | 5 | 17 | 35 | 23 | 29 | 24 | 16 | 6 | 35 | 6 | 5 | 14 | 12 |
Mitochondria | 142 | 177 | 34 | 77 | 106 | 162 | 282 | – | 231 | 182 | 248 | 265 | 48 | 63 | 54 | 28 | 16 | 25 | 129 | 232 | 97 | 55 | 163 | 142 |
Nucleus | 0 | 3 | 0 | 1 | 4 | 7 | 7 | – | 5 | 5 | 6 | 5 | 2 | 0 | 1 | 6 | 4 | 7 | 0 | 0 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 2 | 2 | 0 | 1 | 1 | 4 | 12 | – | 5 | 7 | 3 | 12 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 |
Nucleolus | 8 | 0 | 0 | 0 | 0 | 6 | 7 | – | 5 | 2 | 5 | 7 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Peroxisomes | 4 | 8 | 2 | 3 | 10 | 5 | 11 | – | 3 | 4 | 1 | 2 | 9 | 12 | 28 | 3 | 2 | 1 | 6 | 9 | 11 | 2 | 6 | 5 |
SpindlePole | 5 | 3 | 2 | 7 | 16 | 10 | 30 | – | 15 | 3 | 5 | 15 | 5 | 3 | 7 | 15 | 11 | 11 | 1 | 0 | 0 | 0 | 2 | 2 |
Vac/Vac Membrane | 17 | 49 | 16 | 8 | 28 | 21 | 43 | – | 33 | 22 | 19 | 23 | 19 | 14 | 18 | 96 | 61 | 83 | 2 | 15 | 2 | 7 | 20 | 25 |
Unique Cell Count | 161 | 266 | 71 | 109 | 201 | 222 | 384 | 270 | 216 | 274 | 304 | 116 | 169 | 193 | 308 | 242 | 241 | 168 | 340 | 142 | 88 | 245 | 240 | |
Labelled Cell Count | 208 | 359 | 90 | 143 | 255 | 271 | 485 | 343 | 250 | 317 | 376 | 160 | 229 | 231 | 406 | 296 | 310 | 168 | 340 | 142 | 88 | 245 | 240 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 7.2 | 6.9 | 6.5 | 6.8 | 6.0 | 6.2 | – | 5.5 | 5.5 | 5.0 | 5.2 | 7.2 | 7.1 | 6.8 | 9.5 | 9.4 | 9.2 | 6.0 | 6.5 | 7.0 |
Std Deviation (1e-4) | 0.8 | 1.1 | 1.9 | 1.4 | 1.4 | 1.5 | 1.6 | – | 1.4 | 1.2 | 1.6 | 1.0 | 1.4 | 1.4 | 1.4 | 1.8 | 1.9 | 1.6 | 1.0 | 1.0 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.03 | -0.22 | -0.16 | – | -0.34 | -0.33 | -0.48 | -0.42 | 0.04 | 0.03 | -0.04 | 0.45 | 0.44 | 0.41 | -0.2 | -0.09 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.9 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | -0.8 | 0.1 | 0 | 0 | -0.4 | -1.6 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 2.0 | 0 |
Cytoplasm | 1.4 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3.0 | 2.8 | 3.1 | 4.5 | 4.8 | 4.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -3.0 | 0.2 | -2.2 | -1.6 | – | -2.8 | 0 | 0 | 0 | -0.7 | -0.4 | 0.3 | -0.9 | -1.3 | -0.6 |
Golgi | 0 | -1.2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0.4 | 1.5 | -0.2 | -0.8 | -0.7 | -1.6 |
Mitochondria | 3.1 | 0.7 | 3.9 | 4.3 | – | 6.8 | 6.2 | 8.3 | 7.4 | -0.9 | -1.5 | -3.0 | -8.0 | -8.3 | -7.1 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.9 | -1.7 | -2.9 | -2.6 | – | -2.2 | -2.7 | -3.9 | -3.7 | -1.0 | -3.0 | -2.8 | 1.4 | 0.5 | 1.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.4524 | 0.9526 | 0.9225 | 1.1275 | 0.5743 | 0.6919 | 1.6017 | 2.5525 | 2.0557 | 1.7496 | 1.1543 | 2.0806 | 1.1225 | 1.6155 | 1.2121 | 1.0589 | 0.687 | 1.1563 |
Actin | 0.0273 | 0.0426 | 0.0738 | 0.0271 | 0.0556 | 0.0233 | 0.0386 | 0.0243 | 0.0166 | 0.0089 | 0.0046 | 0.0169 | 0.0319 | 0.0061 | 0.0196 | 0.0068 | 0.0193 | 0.0151 |
Bud | 0.0013 | 0.005 | 0.0032 | 0.0171 | 0.0009 | 0.0011 | 0.0022 | 0.0035 | 0.0151 | 0.0269 | 0.001 | 0.0022 | 0.0015 | 0.0024 | 0.0032 | 0.001 | 0.0048 | 0.0009 |
Bud Neck | 0.0025 | 0.0016 | 0.0045 | 0.0035 | 0.0008 | 0.0025 | 0.0056 | 0.0015 | 0.0013 | 0.0015 | 0.0005 | 0.002 | 0.0017 | 0.0007 | 0.0023 | 0.0009 | 0.0013 | 0.0017 |
Bud Periphery | 0.0019 | 0.0045 | 0.0034 | 0.0186 | 0.0009 | 0.0018 | 0.0024 | 0.0038 | 0.0159 | 0.0445 | 0.0024 | 0.0026 | 0.0027 | 0.0031 | 0.0063 | 0.0015 | 0.0076 | 0.0016 |
Bud Site | 0.0134 | 0.0281 | 0.0172 | 0.0112 | 0.0016 | 0.0013 | 0.0201 | 0.0382 | 0.0167 | 0.0116 | 0.0009 | 0.0018 | 0.0074 | 0.0093 | 0.0112 | 0.0014 | 0.0029 | 0.0015 |
Cell Periphery | 0.0007 | 0.001 | 0.0011 | 0.0016 | 0.0001 | 0.0006 | 0.0013 | 0.0012 | 0.0021 | 0.0017 | 0.0003 | 0.0005 | 0.0007 | 0.0009 | 0.0019 | 0.0002 | 0.0005 | 0.0003 |
Cytoplasm | 0.0064 | 0.0643 | 0.0359 | 0.0317 | 0.0037 | 0.0068 | 0.0194 | 0.0479 | 0.0349 | 0.0456 | 0.0017 | 0.0173 | 0.0144 | 0.0295 | 0.0235 | 0.0093 | 0.0096 | 0.0108 |
Cytoplasmic Foci | 0.0761 | 0.0704 | 0.0715 | 0.0617 | 0.0659 | 0.0592 | 0.0732 | 0.0988 | 0.0601 | 0.0739 | 0.0337 | 0.0549 | 0.0677 | 0.1001 | 0.0433 | 0.0651 | 0.0698 | 0.0664 |
Eisosomes | 0.0006 | 0.0004 | 0.001 | 0.0019 | 0.0002 | 0.0004 | 0.0008 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0008 | 0.0004 | 0.0007 | 0.0002 | 0.0003 | 0.0003 |
Endoplasmic Reticulum | 0.0084 | 0.002 | 0.0034 | 0.0065 | 0.0007 | 0.0048 | 0.0068 | 0.0055 | 0.0026 | 0.002 | 0.0003 | 0.004 | 0.0057 | 0.004 | 0.0026 | 0.0011 | 0.0024 | 0.0023 |
Endosome | 0.1974 | 0.14 | 0.1078 | 0.1378 | 0.2086 | 0.1073 | 0.1638 | 0.1337 | 0.095 | 0.0672 | 0.0563 | 0.1047 | 0.1536 | 0.1543 | 0.0802 | 0.1617 | 0.0967 | 0.127 |
Golgi | 0.098 | 0.0832 | 0.0887 | 0.0922 | 0.0954 | 0.118 | 0.0767 | 0.1071 | 0.0796 | 0.0715 | 0.1095 | 0.1086 | 0.1091 | 0.141 | 0.0652 | 0.137 | 0.1287 | 0.1209 |
Lipid Particles | 0.0541 | 0.0219 | 0.0104 | 0.0213 | 0.0087 | 0.0129 | 0.0516 | 0.009 | 0.0087 | 0.0345 | 0.0029 | 0.0114 | 0.0237 | 0.0146 | 0.0097 | 0.0103 | 0.0139 | 0.0139 |
Mitochondria | 0.4504 | 0.4447 | 0.5036 | 0.4313 | 0.5015 | 0.6125 | 0.4381 | 0.4565 | 0.583 | 0.4948 | 0.7247 | 0.6291 | 0.4936 | 0.4712 | 0.6857 | 0.5272 | 0.5597 | 0.5768 |
None | 0.0049 | 0.0014 | 0.0245 | 0.0734 | 0.0002 | 0.0048 | 0.0188 | 0.0126 | 0.0156 | 0.0096 | 0.0004 | 0.0077 | 0.0207 | 0.0082 | 0.0029 | 0.0018 | 0.0031 | 0.0109 |
Nuclear Periphery | 0.0065 | 0.0007 | 0.0019 | 0.0092 | 0.0003 | 0.0011 | 0.0107 | 0.0016 | 0.0007 | 0.0012 | 0.0001 | 0.0015 | 0.0099 | 0.0014 | 0.0006 | 0.0015 | 0.0011 | 0.0026 |
Nucleolus | 0.0016 | 0.0013 | 0.0003 | 0.0016 | 0.0001 | 0.0002 | 0.0018 | 0.0014 | 0.0008 | 0.0016 | 0.0001 | 0.0004 | 0.0011 | 0.0007 | 0.0002 | 0.0005 | 0.0002 | 0.0002 |
Nucleus | 0.0013 | 0.0007 | 0.0007 | 0.0067 | 0.0002 | 0.0005 | 0.0029 | 0.0013 | 0.0014 | 0.0026 | 0.0001 | 0.0006 | 0.0014 | 0.0009 | 0.0005 | 0.0011 | 0.0006 | 0.0005 |
Peroxisomes | 0.0264 | 0.06 | 0.0363 | 0.0177 | 0.0465 | 0.0281 | 0.0405 | 0.0347 | 0.0368 | 0.0872 | 0.0576 | 0.0257 | 0.0328 | 0.0318 | 0.033 | 0.0397 | 0.0708 | 0.0383 |
Punctate Nuclear | 0.0029 | 0.0004 | 0.0004 | 0.0129 | 0.0003 | 0.0003 | 0.0024 | 0.0013 | 0.0009 | 0.0015 | 0.0001 | 0.0007 | 0.0042 | 0.001 | 0.0003 | 0.0243 | 0.0005 | 0.0008 |
Vacuole | 0.0112 | 0.0199 | 0.0066 | 0.0084 | 0.0036 | 0.006 | 0.015 | 0.0107 | 0.0072 | 0.0058 | 0.0012 | 0.0033 | 0.0081 | 0.0116 | 0.0041 | 0.0035 | 0.0037 | 0.0036 |
Vacuole Periphery | 0.0065 | 0.0058 | 0.0036 | 0.0064 | 0.0045 | 0.0065 | 0.0073 | 0.005 | 0.0044 | 0.0059 | 0.0015 | 0.0038 | 0.0072 | 0.0069 | 0.003 | 0.0038 | 0.0025 | 0.0037 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.5392 | 7.9227 | 5.1441 | 9.6543 | 6.1045 | 6.0897 | 9.5126 | 10.1374 | 8.2021 | 11.2495 |
Translational Efficiency | 0.5375 | 0.4211 | 0.5726 | 0.3253 | 0.4039 | 0.4959 | 0.4348 | 0.2673 | 0.502 | 0.4275 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2648 | 1059 | 247 | 1352 | 1128 | 1902 | 197 | 899 | 3776 | 2961 | 444 | 2251 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 673.20 | 759.80 | 924.24 | 913.28 | 783.66 | 786.73 | 1061.25 | 913.46 | 706.20 | 777.10 | 985.03 | 913.35 |
Standard Deviation | 75.52 | 112.00 | 118.65 | 112.85 | 94.32 | 108.13 | 148.20 | 112.73 | 95.99 | 110.29 | 149.03 | 112.80 |
Intensity Change Log 2 | — | 0.174585 | 0.457232 | 0.440022 | — | 0.005641 | 0.437465 | 0.221114 | — | 0.086173 | 0.446633 | 0.326409 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000357 | 0.001154 | 0.011581 | 0.044009 | 0.001073 | 0.001872 | 0.016159 | 0.010180 | 0.000571 | 0.001615 | 0.013612 | 0.030499 |
Bud Neck | 0.023351 | 0.008628 | 0.014037 | 0.004094 | 0.007545 | 0.005449 | 0.013741 | 0.006106 | 0.018630 | 0.006586 | 0.013905 | 0.004897 |
Bud Site | 0.024678 | 0.022832 | 0.077381 | 0.089398 | 0.011529 | 0.008390 | 0.063060 | 0.045367 | 0.020750 | 0.013555 | 0.071027 | 0.071813 |
Cell Periphery | 0.000323 | 0.000505 | 0.000381 | 0.001146 | 0.000337 | 0.000259 | 0.000417 | 0.000366 | 0.000328 | 0.000347 | 0.000397 | 0.000835 |
Cytoplasm | 0.030852 | 0.011401 | 0.014564 | 0.023942 | 0.007012 | 0.002823 | 0.017163 | 0.014319 | 0.023730 | 0.005891 | 0.015717 | 0.020099 |
Cytoplasmic Foci | 0.106340 | 0.060651 | 0.107123 | 0.083957 | 0.059831 | 0.045013 | 0.158105 | 0.092881 | 0.092447 | 0.050606 | 0.129743 | 0.087521 |
Eisosomes | 0.000092 | 0.000085 | 0.000124 | 0.000219 | 0.000082 | 0.000082 | 0.000148 | 0.000151 | 0.000089 | 0.000083 | 0.000134 | 0.000192 |
Endoplasmic Reticulum | 0.001570 | 0.001023 | 0.007889 | 0.001442 | 0.000722 | 0.000329 | 0.003200 | 0.002228 | 0.001316 | 0.000577 | 0.005809 | 0.001756 |
Endosome | 0.105634 | 0.095084 | 0.111307 | 0.065576 | 0.102715 | 0.097783 | 0.134688 | 0.125409 | 0.104762 | 0.096818 | 0.121681 | 0.089472 |
Golgi | 0.082125 | 0.126319* | 0.101408 | 0.161604* | 0.127739* | 0.133546* | 0.113986* | 0.145902* | 0.095751 | 0.130961* | 0.106989 | 0.155333* |
Lipid Particles | 0.011486 | 0.002924 | 0.003440 | 0.000683 | 0.005625 | 0.003845 | 0.004359 | 0.002955 | 0.009735 | 0.003515 | 0.003848 | 0.001590 |
Mitochondria | 0.484363* | 0.559355* | 0.175150* | 0.349966* | 0.575127* | 0.623156* | 0.114748* | 0.360050* | 0.511477* | 0.600338* | 0.148350* | 0.353993* |
Mitotic Spindle | 0.014083 | 0.023565 | 0.140458* | 0.051740 | 0.013331 | 0.010504 | 0.120332* | 0.041962 | 0.013858 | 0.015175 | 0.131529* | 0.047834 |
None | 0.002110 | 0.000515 | 0.000300 | 0.000174 | 0.000516 | 0.000329 | 0.000197 | 0.000391 | 0.001634 | 0.000396 | 0.000254 | 0.000261 |
Nuclear Periphery | 0.000469 | 0.000110 | 0.000683 | 0.000116 | 0.000254 | 0.000223 | 0.001387 | 0.000371 | 0.000405 | 0.000183 | 0.000995 | 0.000218 |
Nuclear Periphery Foci | 0.000436 | 0.000345 | 0.001933 | 0.000443 | 0.000417 | 0.000274 | 0.003293 | 0.000609 | 0.000430 | 0.000299 | 0.002537 | 0.000509 |
Nucleolus | 0.002410 | 0.001098 | 0.001121 | 0.000050 | 0.000419 | 0.000915 | 0.001426 | 0.000209 | 0.001815 | 0.000980 | 0.001256 | 0.000113 |
Nucleus | 0.005958 | 0.001778 | 0.020813 | 0.000259 | 0.000855 | 0.000730 | 0.007494 | 0.001139 | 0.004434 | 0.001104 | 0.014903 | 0.000611 |
Peroxisomes | 0.023810 | 0.041664 | 0.103231* | 0.090229 | 0.044320 | 0.030205 | 0.119401* | 0.038391 | 0.029937 | 0.034304 | 0.110405* | 0.069526 |
Vacuole | 0.051035 | 0.020122 | 0.080951 | 0.021275 | 0.018175 | 0.017430 | 0.075837 | 0.073449 | 0.041218 | 0.018393 | 0.078682 | 0.042112 |
Vacuole Periphery | 0.028517 | 0.020841 | 0.026124 | 0.009678 | 0.022375 | 0.016846 | 0.030860 | 0.037564 | 0.026683 | 0.018275 | 0.028225 | 0.020815 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.10 | -3.94 | -12.00 | -11.77 | -6.95 | -1.68 | -3.51 | -4.91 | -4.43 | 1.27 | -3.79 | -5.27 | -12.84 | -12.32 | -4.94 |
Bud Neck | 7.57 | 3.03 | 13.65 | 2.92 | 4.84 | 1.23 | -2.01 | 0.67 | -0.66 | 2.54 | 9.43 | 1.91 | 11.87 | 1.67 | 5.28 |
Bud Site | 0.65 | -6.70 | -14.26 | -13.76 | -0.99 | 1.82 | -6.65 | -8.82 | -10.13 | 2.12 | 4.84 | -9.00 | -16.08 | -18.56 | 0.15 |
Cell Periphery | -0.93 | -0.99 | -2.40 | -1.62 | -2.20 | 1.33 | -0.65 | -0.43 | -2.70 | 0.45 | -0.27 | -1.15 | -2.44 | -2.23 | -2.05 |
Cytoplasm | 8.05 | 3.98 | 2.91 | -5.78 | -2.14 | 2.99 | -3.08 | -4.20 | -8.14 | 0.78 | 12.07 | 2.41 | 1.86 | -11.74 | -1.25 |
Cytoplasmic Foci | 11.45 | 0.33 | 5.60 | -5.75 | 2.70 | 4.54 | -9.09 | -6.91 | -11.27 | 5.78 | 15.63 | -5.26 | 1.71 | -13.24 | 6.09 |
Eisosomes | 1.30 | -3.62 | -12.08 | -12.15 | -6.70 | 0.01 | -3.80 | -4.67 | -4.43 | -0.04 | 1.04 | -5.06 | -11.91 | -11.26 | -4.40 |
Endoplasmic Reticulum | 1.01 | -3.57 | 0.32 | -0.80 | 3.61 | 1.45 | -3.85 | -3.62 | -5.53 | 1.49 | 2.90 | -4.29 | -1.74 | -4.44 | 3.89 |
Endosome | 2.05 | -0.21 | 10.06 | 6.06 | 4.84 | 0.86 | -2.83 | -3.20 | -4.40 | 0.75 | 2.17 | -2.07 | 4.25 | 2.05 | 4.33 |
Golgi | -7.50 | -1.44 | -12.77 | -4.53 | -5.78 | -0.85 | 1.43 | -2.06 | -1.46 | -2.73 | -8.43 | -0.93 | -12.07 | -4.38 | -6.22 |
Lipid Particles | 6.85 | 7.02 | 12.31 | 2.40 | 4.78 | 1.31 | 0.63 | 2.07 | 0.97 | 1.56 | 6.99 | 6.18 | 11.02 | 3.13 | 4.38 |
Mitochondria | -5.90 | 17.32 | 11.52 | 14.46 | -9.35 | -3.58 | 24.10 | 13.87 | 18.90 | -12.46 | -10.32 | 27.93 | 17.01 | 25.25 | -15.03 |
Mitotic Spindle | -3.64 | -8.95 | -10.68 | -6.91 | 6.13 | 1.33 | -7.19 | -6.94 | -7.97 | 5.15 | -0.91 | -11.47 | -12.71 | -11.84 | 7.97 |
None | 4.27 | 5.11 | 5.62 | 2.20 | 2.73 | 0.75 | 1.29 | 0.46 | -0.33 | -1.16 | 4.75 | 5.35 | 5.31 | 1.44 | 0.20 |
Nuclear Periphery | 5.11 | -1.78 | 4.63 | -0.21 | 4.32 | 0.24 | -2.54 | -0.98 | -1.43 | 2.30 | 2.91 | -2.90 | 2.81 | -0.60 | 3.83 |
Nuclear Periphery Foci | 1.23 | -3.81 | 0.30 | -0.72 | 3.86 | 0.80 | -3.68 | -0.98 | -2.73 | 3.50 | 1.81 | -5.20 | -0.34 | -2.19 | 5.11 |
Nucleolus | 2.29 | 2.65 | 6.83 | 2.14 | 3.61 | -1.35 | -2.28 | 1.62 | 1.91 | 2.73 | 2.35 | 1.47 | 6.90 | 2.98 | 4.47 |
Nucleus | 5.37 | -2.83 | 9.59 | 2.96 | 3.72 | 0.40 | -2.22 | -1.48 | -1.69 | 1.90 | 6.94 | -3.26 | 7.16 | 0.58 | 4.18 |
Peroxisomes | -4.83 | -6.05 | -13.63 | -8.35 | 0.80 | 3.35 | -5.27 | 1.21 | -2.04 | 5.71 | -1.82 | -8.38 | -11.39 | -9.58 | 4.01 |
Vacuole | 12.34 | -4.09 | 10.34 | -0.88 | 7.18 | 0.36 | -6.49 | -11.56 | -11.87 | 0.65 | 12.91 | -6.58 | -0.87 | -10.24 | 6.05 |
Vacuole Periphery | 3.79 | 0.96 | 13.67 | 6.04 | 4.47 | 2.68 | -1.48 | -4.67 | -7.16 | -1.28 | 6.40 | -0.22 | 3.87 | -1.62 | 2.12 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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