Standard name
Human Ortholog
Description Protein involved in splicing Group I aI5-beta intron from COX1 mRNA; mitochondrial matrix protein

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.11 0.07 0 0 0 0 0 0 0 0.08 0.12 0 0.11 0.1 0.06 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.07 0 0 0 0 0 0 0 0 0 0.07 0 0.06 0.13 0.12 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0.1 0.07 0.06 0 0 0 0.05 0 0.2 0.18 0.2 0.3 0.33 0.33 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.07 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.07 0 0 0 0 0 0
Endosome 0.07 0 0.18 0.05 0.19 0.09 0.11 0.07 0 0 0 0.15 0.16 0.2 0.14 0.12 0.15 0.09 0.08 0.08 0.13 0.06 0.08
Golgi 0 0.21 0.13 0.13 0.08 0 0 0 0 0 0 0.15 0.21 0.12 0.09 0.1 0.07 0 0.1 0.05 0.06 0.06 0.05
Mitochondria 0.88 0.67 0.48 0.71 0.53 0.73 0.73 0.86 0.84 0.91 0.87 0.41 0.37 0.28 0.09 0.07 0.1 0.77 0.68 0.69 0.63 0.67 0.59
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0 0 0 0 0 0.08 0.07 0.15 0 0 0 0 0 0.08 0 0 0
SpindlePole 0 0 0 0.06 0.08 0.05 0.08 0.06 0 0 0.05 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.18 0.23 0.07 0.14 0.09 0.11 0.12 0.1 0.07 0.08 0.16 0.08 0.09 0.31 0.25 0.34 0 0.05 0 0.08 0.08 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 8 8 8 6 1 3 1 4 0 1 9 20 2 35 23 14 2 4 4 0 5 7
Bud 5 2 0 4 4 7 8 6 2 5 11 0 5 4 3 6 5 0 2 1 0 1 1
Bud Neck 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 1 0 0 1 0 0 2 4
Bud Site 1 0 0 0 0 3 2 3 2 3 4 0 0 0 0 0 1
Cell Periphery 3 18 3 2 4 3 5 5 2 2 5 8 7 11 41 30 15 0 0 0 0 0 0
Cytoplasm 5 8 3 11 14 14 15 7 7 15 7 23 30 38 93 79 79 0 1 0 1 3 7
Endoplasmic Reticulum 1 18 0 1 6 5 7 1 3 3 5 3 12 6 11 8 16 1 4 0 0 2 1
Endosome 11 6 13 5 39 20 44 20 3 2 9 17 27 38 44 30 37 14 26 11 11 15 19
Golgi 4 57 9 14 16 3 8 3 2 0 5 17 35 23 29 24 16 6 35 6 5 14 12
Mitochondria 142 177 34 77 106 162 282 231 182 248 265 48 63 54 28 16 25 129 232 97 55 163 142
Nucleus 0 3 0 1 4 7 7 5 5 6 5 2 0 1 6 4 7 0 0 0 1 0 1
Nuclear Periphery 2 2 0 1 1 4 12 5 7 3 12 0 1 1 1 0 0 0 1 1 0 2 1
Nucleolus 8 0 0 0 0 6 7 5 2 5 7 0 0 0 1 1 0 1 0 0 0 1 1
Peroxisomes 4 8 2 3 10 5 11 3 4 1 2 9 12 28 3 2 1 6 9 11 2 6 5
SpindlePole 5 3 2 7 16 10 30 15 3 5 15 5 3 7 15 11 11 1 0 0 0 2 2
Vac/Vac Membrane 17 49 16 8 28 21 43 33 22 19 23 19 14 18 96 61 83 2 15 2 7 20 25
Unique Cell Count 161 266 71 109 201 222 384 270 216 274 304 116 169 193 308 242 241 168 340 142 88 245 240
Labelled Cell Count 208 359 90 143 255 271 485 343 250 317 376 160 229 231 406 296 310 168 340 142 88 245 240


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 7.2 6.9 6.5 6.8 6.0 6.2 5.5 5.5 5.0 5.2 7.2 7.1 6.8 9.5 9.4 9.2 6.0 6.5 7.0
Std Deviation (1e-4) 0.8 1.1 1.9 1.4 1.4 1.5 1.6 1.4 1.2 1.6 1.0 1.4 1.4 1.4 1.8 1.9 1.6 1.0 1.0 1.3
Intensity Change (Log2) -0.1 -0.03 -0.22 -0.16 -0.34 -0.33 -0.48 -0.42 0.04 0.03 -0.04 0.45 0.44 0.41 -0.2 -0.09 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 0 0 0 0 0 0 0 -0.8 0.1 0 0 -0.4 -1.6
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 2.2 2.0 0
Cytoplasm 1.4 0 0 0 0 0 0 0 3.0 2.8 3.1 4.5 4.8 4.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -3.0 0.2 -2.2 -1.6 -2.8 0 0 0 -0.7 -0.4 0.3 -0.9 -1.3 -0.6
Golgi 0 -1.2 0 0 0 0 0 0 0.4 1.5 -0.2 -0.8 -0.7 -1.6
Mitochondria 3.1 0.7 3.9 4.3 6.8 6.2 8.3 7.4 -0.9 -1.5 -3.0 -8.0 -8.3 -7.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 2.7 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.9 -1.7 -2.9 -2.6 -2.2 -2.7 -3.9 -3.7 -1.0 -3.0 -2.8 1.4 0.5 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4524 0.9526 0.9225 1.1275 0.5743 0.6919 1.6017 2.5525 2.0557 1.7496 1.1543 2.0806 1.1225 1.6155 1.2121 1.0589 0.687 1.1563
Actin 0.0273 0.0426 0.0738 0.0271 0.0556 0.0233 0.0386 0.0243 0.0166 0.0089 0.0046 0.0169 0.0319 0.0061 0.0196 0.0068 0.0193 0.0151
Bud 0.0013 0.005 0.0032 0.0171 0.0009 0.0011 0.0022 0.0035 0.0151 0.0269 0.001 0.0022 0.0015 0.0024 0.0032 0.001 0.0048 0.0009
Bud Neck 0.0025 0.0016 0.0045 0.0035 0.0008 0.0025 0.0056 0.0015 0.0013 0.0015 0.0005 0.002 0.0017 0.0007 0.0023 0.0009 0.0013 0.0017
Bud Periphery 0.0019 0.0045 0.0034 0.0186 0.0009 0.0018 0.0024 0.0038 0.0159 0.0445 0.0024 0.0026 0.0027 0.0031 0.0063 0.0015 0.0076 0.0016
Bud Site 0.0134 0.0281 0.0172 0.0112 0.0016 0.0013 0.0201 0.0382 0.0167 0.0116 0.0009 0.0018 0.0074 0.0093 0.0112 0.0014 0.0029 0.0015
Cell Periphery 0.0007 0.001 0.0011 0.0016 0.0001 0.0006 0.0013 0.0012 0.0021 0.0017 0.0003 0.0005 0.0007 0.0009 0.0019 0.0002 0.0005 0.0003
Cytoplasm 0.0064 0.0643 0.0359 0.0317 0.0037 0.0068 0.0194 0.0479 0.0349 0.0456 0.0017 0.0173 0.0144 0.0295 0.0235 0.0093 0.0096 0.0108
Cytoplasmic Foci 0.0761 0.0704 0.0715 0.0617 0.0659 0.0592 0.0732 0.0988 0.0601 0.0739 0.0337 0.0549 0.0677 0.1001 0.0433 0.0651 0.0698 0.0664
Eisosomes 0.0006 0.0004 0.001 0.0019 0.0002 0.0004 0.0008 0.0003 0.0005 0.0002 0.0002 0.0003 0.0008 0.0004 0.0007 0.0002 0.0003 0.0003
Endoplasmic Reticulum 0.0084 0.002 0.0034 0.0065 0.0007 0.0048 0.0068 0.0055 0.0026 0.002 0.0003 0.004 0.0057 0.004 0.0026 0.0011 0.0024 0.0023
Endosome 0.1974 0.14 0.1078 0.1378 0.2086 0.1073 0.1638 0.1337 0.095 0.0672 0.0563 0.1047 0.1536 0.1543 0.0802 0.1617 0.0967 0.127
Golgi 0.098 0.0832 0.0887 0.0922 0.0954 0.118 0.0767 0.1071 0.0796 0.0715 0.1095 0.1086 0.1091 0.141 0.0652 0.137 0.1287 0.1209
Lipid Particles 0.0541 0.0219 0.0104 0.0213 0.0087 0.0129 0.0516 0.009 0.0087 0.0345 0.0029 0.0114 0.0237 0.0146 0.0097 0.0103 0.0139 0.0139
Mitochondria 0.4504 0.4447 0.5036 0.4313 0.5015 0.6125 0.4381 0.4565 0.583 0.4948 0.7247 0.6291 0.4936 0.4712 0.6857 0.5272 0.5597 0.5768
None 0.0049 0.0014 0.0245 0.0734 0.0002 0.0048 0.0188 0.0126 0.0156 0.0096 0.0004 0.0077 0.0207 0.0082 0.0029 0.0018 0.0031 0.0109
Nuclear Periphery 0.0065 0.0007 0.0019 0.0092 0.0003 0.0011 0.0107 0.0016 0.0007 0.0012 0.0001 0.0015 0.0099 0.0014 0.0006 0.0015 0.0011 0.0026
Nucleolus 0.0016 0.0013 0.0003 0.0016 0.0001 0.0002 0.0018 0.0014 0.0008 0.0016 0.0001 0.0004 0.0011 0.0007 0.0002 0.0005 0.0002 0.0002
Nucleus 0.0013 0.0007 0.0007 0.0067 0.0002 0.0005 0.0029 0.0013 0.0014 0.0026 0.0001 0.0006 0.0014 0.0009 0.0005 0.0011 0.0006 0.0005
Peroxisomes 0.0264 0.06 0.0363 0.0177 0.0465 0.0281 0.0405 0.0347 0.0368 0.0872 0.0576 0.0257 0.0328 0.0318 0.033 0.0397 0.0708 0.0383
Punctate Nuclear 0.0029 0.0004 0.0004 0.0129 0.0003 0.0003 0.0024 0.0013 0.0009 0.0015 0.0001 0.0007 0.0042 0.001 0.0003 0.0243 0.0005 0.0008
Vacuole 0.0112 0.0199 0.0066 0.0084 0.0036 0.006 0.015 0.0107 0.0072 0.0058 0.0012 0.0033 0.0081 0.0116 0.0041 0.0035 0.0037 0.0036
Vacuole Periphery 0.0065 0.0058 0.0036 0.0064 0.0045 0.0065 0.0073 0.005 0.0044 0.0059 0.0015 0.0038 0.0072 0.0069 0.003 0.0038 0.0025 0.0037

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.5392 7.9227 5.1441 9.6543 6.1045 6.0897 9.5126 10.1374 8.2021 11.2495
Translational Efficiency 0.5375 0.4211 0.5726 0.3253 0.4039 0.4959 0.4348 0.2673 0.502 0.4275

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2648 1059 247 1352 1128 1902 197 899 3776 2961 444 2251

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 673.20 759.80 924.24 913.28 783.66 786.73 1061.25 913.46 706.20 777.10 985.03 913.35
Standard Deviation 75.52 112.00 118.65 112.85 94.32 108.13 148.20 112.73 95.99 110.29 149.03 112.80
Intensity Change Log 2 0.174585 0.457232 0.440022 0.005641 0.437465 0.221114 0.086173 0.446633 0.326409

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000357 0.001154 0.011581 0.044009 0.001073 0.001872 0.016159 0.010180 0.000571 0.001615 0.013612 0.030499
Bud Neck 0.023351 0.008628 0.014037 0.004094 0.007545 0.005449 0.013741 0.006106 0.018630 0.006586 0.013905 0.004897
Bud Site 0.024678 0.022832 0.077381 0.089398 0.011529 0.008390 0.063060 0.045367 0.020750 0.013555 0.071027 0.071813
Cell Periphery 0.000323 0.000505 0.000381 0.001146 0.000337 0.000259 0.000417 0.000366 0.000328 0.000347 0.000397 0.000835
Cytoplasm 0.030852 0.011401 0.014564 0.023942 0.007012 0.002823 0.017163 0.014319 0.023730 0.005891 0.015717 0.020099
Cytoplasmic Foci 0.106340 0.060651 0.107123 0.083957 0.059831 0.045013 0.158105 0.092881 0.092447 0.050606 0.129743 0.087521
Eisosomes 0.000092 0.000085 0.000124 0.000219 0.000082 0.000082 0.000148 0.000151 0.000089 0.000083 0.000134 0.000192
Endoplasmic Reticulum 0.001570 0.001023 0.007889 0.001442 0.000722 0.000329 0.003200 0.002228 0.001316 0.000577 0.005809 0.001756
Endosome 0.105634 0.095084 0.111307 0.065576 0.102715 0.097783 0.134688 0.125409 0.104762 0.096818 0.121681 0.089472
Golgi 0.082125 0.126319* 0.101408 0.161604* 0.127739* 0.133546* 0.113986* 0.145902* 0.095751 0.130961* 0.106989 0.155333*
Lipid Particles 0.011486 0.002924 0.003440 0.000683 0.005625 0.003845 0.004359 0.002955 0.009735 0.003515 0.003848 0.001590
Mitochondria 0.484363* 0.559355* 0.175150* 0.349966* 0.575127* 0.623156* 0.114748* 0.360050* 0.511477* 0.600338* 0.148350* 0.353993*
Mitotic Spindle 0.014083 0.023565 0.140458* 0.051740 0.013331 0.010504 0.120332* 0.041962 0.013858 0.015175 0.131529* 0.047834
None 0.002110 0.000515 0.000300 0.000174 0.000516 0.000329 0.000197 0.000391 0.001634 0.000396 0.000254 0.000261
Nuclear Periphery 0.000469 0.000110 0.000683 0.000116 0.000254 0.000223 0.001387 0.000371 0.000405 0.000183 0.000995 0.000218
Nuclear Periphery Foci 0.000436 0.000345 0.001933 0.000443 0.000417 0.000274 0.003293 0.000609 0.000430 0.000299 0.002537 0.000509
Nucleolus 0.002410 0.001098 0.001121 0.000050 0.000419 0.000915 0.001426 0.000209 0.001815 0.000980 0.001256 0.000113
Nucleus 0.005958 0.001778 0.020813 0.000259 0.000855 0.000730 0.007494 0.001139 0.004434 0.001104 0.014903 0.000611
Peroxisomes 0.023810 0.041664 0.103231* 0.090229 0.044320 0.030205 0.119401* 0.038391 0.029937 0.034304 0.110405* 0.069526
Vacuole 0.051035 0.020122 0.080951 0.021275 0.018175 0.017430 0.075837 0.073449 0.041218 0.018393 0.078682 0.042112
Vacuole Periphery 0.028517 0.020841 0.026124 0.009678 0.022375 0.016846 0.030860 0.037564 0.026683 0.018275 0.028225 0.020815

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.10 -3.94 -12.00 -11.77 -6.95 -1.68 -3.51 -4.91 -4.43 1.27 -3.79 -5.27 -12.84 -12.32 -4.94
Bud Neck 7.57 3.03 13.65 2.92 4.84 1.23 -2.01 0.67 -0.66 2.54 9.43 1.91 11.87 1.67 5.28
Bud Site 0.65 -6.70 -14.26 -13.76 -0.99 1.82 -6.65 -8.82 -10.13 2.12 4.84 -9.00 -16.08 -18.56 0.15
Cell Periphery -0.93 -0.99 -2.40 -1.62 -2.20 1.33 -0.65 -0.43 -2.70 0.45 -0.27 -1.15 -2.44 -2.23 -2.05
Cytoplasm 8.05 3.98 2.91 -5.78 -2.14 2.99 -3.08 -4.20 -8.14 0.78 12.07 2.41 1.86 -11.74 -1.25
Cytoplasmic Foci 11.45 0.33 5.60 -5.75 2.70 4.54 -9.09 -6.91 -11.27 5.78 15.63 -5.26 1.71 -13.24 6.09
Eisosomes 1.30 -3.62 -12.08 -12.15 -6.70 0.01 -3.80 -4.67 -4.43 -0.04 1.04 -5.06 -11.91 -11.26 -4.40
Endoplasmic Reticulum 1.01 -3.57 0.32 -0.80 3.61 1.45 -3.85 -3.62 -5.53 1.49 2.90 -4.29 -1.74 -4.44 3.89
Endosome 2.05 -0.21 10.06 6.06 4.84 0.86 -2.83 -3.20 -4.40 0.75 2.17 -2.07 4.25 2.05 4.33
Golgi -7.50 -1.44 -12.77 -4.53 -5.78 -0.85 1.43 -2.06 -1.46 -2.73 -8.43 -0.93 -12.07 -4.38 -6.22
Lipid Particles 6.85 7.02 12.31 2.40 4.78 1.31 0.63 2.07 0.97 1.56 6.99 6.18 11.02 3.13 4.38
Mitochondria -5.90 17.32 11.52 14.46 -9.35 -3.58 24.10 13.87 18.90 -12.46 -10.32 27.93 17.01 25.25 -15.03
Mitotic Spindle -3.64 -8.95 -10.68 -6.91 6.13 1.33 -7.19 -6.94 -7.97 5.15 -0.91 -11.47 -12.71 -11.84 7.97
None 4.27 5.11 5.62 2.20 2.73 0.75 1.29 0.46 -0.33 -1.16 4.75 5.35 5.31 1.44 0.20
Nuclear Periphery 5.11 -1.78 4.63 -0.21 4.32 0.24 -2.54 -0.98 -1.43 2.30 2.91 -2.90 2.81 -0.60 3.83
Nuclear Periphery Foci 1.23 -3.81 0.30 -0.72 3.86 0.80 -3.68 -0.98 -2.73 3.50 1.81 -5.20 -0.34 -2.19 5.11
Nucleolus 2.29 2.65 6.83 2.14 3.61 -1.35 -2.28 1.62 1.91 2.73 2.35 1.47 6.90 2.98 4.47
Nucleus 5.37 -2.83 9.59 2.96 3.72 0.40 -2.22 -1.48 -1.69 1.90 6.94 -3.26 7.16 0.58 4.18
Peroxisomes -4.83 -6.05 -13.63 -8.35 0.80 3.35 -5.27 1.21 -2.04 5.71 -1.82 -8.38 -11.39 -9.58 4.01
Vacuole 12.34 -4.09 10.34 -0.88 7.18 0.36 -6.49 -11.56 -11.87 0.65 12.91 -6.58 -0.87 -10.24 6.05
Vacuole Periphery 3.79 0.96 13.67 6.04 4.47 2.68 -1.48 -4.67 -7.16 -1.28 6.40 -0.22 3.87 -1.62 2.12
Standard name
Human Ortholog
Description Protein involved in splicing Group I aI5-beta intron from COX1 mRNA; mitochondrial matrix protein
Localization
Cell Percentages mitochondrion (88%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mne1

Mne1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mne1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available