Standard name
Human Ortholog
Description Protein of unknown function; required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.09 0.24 0.15 0.19 0.16 0.13 0.14 0.11 0.07 0.39 0.82 0.92 0.3 0.25 0.31 0 0 0 0.06 0.12 0.15
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.19 0.07 0 0 0 0 0 0 0.11 0 0 0.08 0.05 0.1 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0 0.07 0.09 0.15 0.4 0.48 0.47 0.66 0.77 0.86 0.83 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.88 0.91 0.81 0.72 0.22 0.33 0.37 0.47 0.38 0.38 0.25 0.34 0.13 0 0 0.49 0.56 0.43 0.73 0.75 0.6 0.51 0.37 0.28
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.1 0.17 0.13 0.08 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.12 0.08
Vac/Vac Membrane 0 0.07 0.06 0.1 0.37 0.21 0.15 0.11 0.09 0.06 0 0 0.53 0.31 0.17 0.17 0.2 0.26 0 0.07 0.11 0.08 0.13 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 2 2 1 5 0 1 0 0 0 0 0 0 8 6 2 3 8 14
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 4 13
Bud Site 0 1 0 1 0 2 0 1 0 0 0 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 6 4 10 31 21 29 23 12 23 3 6 81 144 270 90 52 52 3 0 1 8 17 21
Endoplasmic Reticulum 1 0 1 0 0 0 0 0 0 0 0 0 1 0 1 13 7 7 0 0 1 0 1 0
Endosome 1 2 3 3 25 9 3 1 0 0 0 1 23 4 4 25 10 17 7 8 3 5 4 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 4 0 0 0 0
Mitochondria 43 8 8 10 19 54 75 69 61 131 24 71 1 1 0 12 3 4 0 3 0 1 1 1
Nucleus 283 299 96 79 29 45 58 68 35 65 7 29 26 1 2 148 115 73 253 283 92 67 52 41
Nuclear Periphery 0 0 0 0 1 0 0 1 2 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0
Nucleolus 30 13 4 3 4 4 1 1 0 5 0 1 0 0 0 1 0 0 46 38 26 17 10 4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 1 1 0 0 1 1 0 0 0 0 7 2 2 8 3 5 9 16 11
Vac/Vac Membrane 7 24 7 11 48 29 24 16 8 11 1 3 110 55 51 53 41 45 12 24 16 10 18 23
Unique Cell Count 323 329 119 109 131 136 156 146 93 170 28 86 206 176 295 305 204 170 346 379 154 132 141 146
Labelled Cell Count 372 353 123 117 158 166 193 182 119 241 36 115 243 205 328 350 230 200 346 379 154 132 141 146


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 7.0 6.1 6.0 5.4 4.8 4.2 4.4 4.4 4.0 3.9 4.0 6.3 5.8 6.0 7.3 8.1 8.1 6.1 5.8 6.2
Std Deviation (1e-4) 0.7 1.1 1.1 1.2 1.4 1.7 1.2 1.4 1.7 1.3 1.0 1.2 1.1 1.3 1.9 1.5 1.4 1.5 1.5 1.1 1.2
Intensity Change (Log2) – – – -0.04 -0.19 -0.34 -0.56 -0.48 -0.48 -0.62 -0.65 -0.63 0.05 -0.09 -0.02 0.24 0.41 0.41 0.0 -0.08 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.8 4.6 3.2 3.9 3.3 2.6 2.9 0 0 7.1 13.2 17.2 5.8 5.1 5.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 4.1 0 0 0 0 0 0 0 2.8 0 0 2.1 0 2.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.7 2.0 0 0 0 0 0 0 0 0 0 0 -1.2 0 0
Nucleus -1.5 -9.2 -7.6 -7.2 -5.7 -6.4 -7.1 -5.8 -6.8 -12.2 -14.4 -17.3 -6.0 -4.4 -6.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.2 5.9 3.5 2.5 1.5 0.8 0.2 0 0 8.6 5.2 3.0 3.1 3.5 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3142 2.794 2.9021 2.4641 2.638 2.5951 5.229 5.1307 4.9416 5.0054 4.9748 4.9547 6.1539 6.2216 5.5358 6.7412 6.1089 6.0647
Actin 0.0118 0.0001 0.0084 0.0001 0.0003 0.0001 0.0193 0.0083 0.0096 0.039 0.0128 0.0015 0.0088 0.0001 0.0074 0 0.0006 0.0007
Bud 0.0002 0.0001 0.0005 0.0001 0.0003 0.0001 0.0009 0.0001 0.0002 0.0022 0.0006 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001
Bud Neck 0.0019 0.0003 0.0009 0.0005 0.0026 0.0029 0.0039 0.0005 0.0005 0.0004 0.0021 0.0022 0.0011 0.0007 0.0009 0.0002 0.0031 0.0031
Bud Periphery 0.0004 0 0.0005 0 0.0003 0.0001 0.0023 0 0.0002 0.0028 0.0014 0.0001 0.0006 0 0.0004 0 0.0003 0.0002
Bud Site 0.0012 0.001 0.0078 0.0002 0.001 0.0003 0.0036 0.0007 0.002 0.0075 0.0043 0.0003 0.0012 0.0009 0.0078 0.0001 0.0002 0.0003
Cell Periphery 0.0002 0 0.0002 0 0.0001 0.0001 0.0006 0 0.0001 0.0002 0.0001 0 0.0001 0 0.0065 0 0.0001 0
Cytoplasm 0.0046 0.0167 0.0028 0.0037 0.0026 0.0067 0.0072 0.0064 0.0103 0.0058 0.0075 0.0028 0.0542 0.0247 0.0104 0.0013 0.0065 0.0011
Cytoplasmic Foci 0.014 0.0001 0.0017 0 0.0001 0.0003 0.0135 0.0008 0.0088 0.0113 0.0212 0.0005 0.0046 0.0005 0.022 0.0544 0.0005 0.0012
Eisosomes 0.0001 0 0.0001 0 0 0 0.0001 0 0.0002 0.0001 0.0002 0 0.0002 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0027 0.0007 0.0006 0.0001 0.0003 0.0006 0.0043 0.0002 0.0011 0.0025 0.0011 0.0006 0.0044 0.0009 0.0011 0 0.0004 0.0001
Endosome 0.0179 0.001 0.0048 0.0001 0.0003 0.0016 0.0231 0.0006 0.0026 0.035 0.024 0.002 0.0266 0.0008 0.0014 0 0.0036 0.0024
Golgi 0.0026 0 0.0028 0 0.0001 0.0001 0.0106 0.0002 0.003 0.0068 0.0132 0.0004 0.0045 0 0.001 0 0.0031 0.0039
Lipid Particles 0.0208 0 0.0032 0 0 0.0003 0.0112 0.0001 0.003 0.0022 0.0391 0.0002 0.0189 0.0001 0.0024 0 0.0003 0.0016
Mitochondria 0.0019 0.0003 0.0009 0.0003 0.0004 0.0002 0.0093 0.0001 0.001 0.009 0.0088 0.0003 0.0066 0.0002 0.0015 0.009 0.1171 0.0073
None 0.0142 0.0021 0.0003 0.0003 0.0002 0.0004 0.0043 0.0021 0.0029 0.0011 0.0204 0.0025 0.0055 0.0154 0.0027 0.0127 0.0027 0.0002
Nuclear Periphery 0.0191 0.0206 0.0028 0.003 0.005 0.0044 0.0273 0.0038 0.0038 0.009 0.0099 0.0019 0.0273 0.0069 0.0029 0.0015 0.0117 0.0022
Nucleolus 0.0271 0.0143 0.0085 0.0124 0.031 0.038 0.0208 0.0099 0.0067 0.0034 0.0151 0.0172 0.012 0.0165 0.0071 0.0077 0.0327 0.0274
Nucleus 0.836 0.9263 0.9365 0.9765 0.9141 0.9275 0.7949 0.9439 0.9216 0.8324 0.7632 0.9616 0.7904 0.9039 0.8991 0.8681 0.6907 0.933
Peroxisomes 0.005 0 0.0006 0 0 0 0.0102 0.0002 0.0137 0.0125 0.012 0.0001 0.0036 0 0.0007 0.0003 0.0002 0.0012
Punctate Nuclear 0.0136 0.0134 0.0111 0.0025 0.0407 0.0154 0.0251 0.0217 0.0077 0.0024 0.0378 0.0054 0.0246 0.0275 0.0236 0.0444 0.1244 0.0134
Vacuole 0.0035 0.0022 0.0047 0.0002 0.0005 0.0009 0.0051 0.0002 0.0008 0.0102 0.0025 0.0002 0.0018 0.0007 0.0008 0.0001 0.0009 0.0004
Vacuole Periphery 0.0013 0.0007 0.0003 0.0001 0.0001 0.0002 0.0023 0 0.0002 0.0041 0.0026 0.0001 0.0028 0.0001 0.0001 0 0.0008 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.6891 37.7199 66.7091 86.874 42.3986 50.6062 77.0717 74.4343 69.9822 82.3294
Translational Efficiency 0.8247 1.3711 0.9512 0.7381 1.2181 1.204 0.7006 0.876 0.9638 0.7973

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
76 1545 267 1569 1224 1454 1554 304 1300 2999 1821 1873

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 780.15 766.20 956.70 837.71 763.22 748.17 924.27 1186.73 764.21 757.46 929.02 894.36
Standard Deviation 93.00 125.53 128.24 119.77 103.00 121.53 117.63 193.85 102.52 123.93 119.80 186.22
Intensity Change Log 2 — -0.026031 0.294315 0.102699 — -0.028733 0.276215 0.636821 — -0.027366 0.285393 0.391439

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000298 0.000764 0.001624 0.001081 0.000277 0.000604 0.000767 0.000083 0.000278 0.000686 0.000893 0.000919
Bud Neck 0.001487 0.014078 0.009713 0.027026 0.002543 0.010498 0.011163 0.015476 0.002481 0.012342 0.010950 0.025151
Bud Site 0.000227 0.003232 0.001767 0.033462 0.000551 0.003423 0.001858 0.001854 0.000532 0.003324 0.001845 0.028332
Cell Periphery 0.000044 0.000150 0.000107 0.000442 0.000088 0.000119 0.000141 0.000077 0.000085 0.000135 0.000136 0.000383
Cytoplasm 0.007210 0.047412 0.003357 0.171384* 0.018065 0.051567 0.026322 0.096183 0.017431 0.049426 0.022955 0.159179*
Cytoplasmic Foci 0.004041 0.002740 0.000507 0.006526 0.002423 0.005206 0.000424 0.000136 0.002517 0.003936 0.000437 0.005489
Eisosomes 0.000022 0.000030 0.000064 0.000032 0.000024 0.000034 0.000032 0.000007 0.000024 0.000032 0.000036 0.000028
Endoplasmic Reticulum 0.000822 0.004959 0.007282 0.004405 0.002331 0.003805 0.004589 0.000499 0.002243 0.004400 0.004984 0.003771
Endosome 0.000584 0.001140 0.000451 0.003747 0.000388 0.001653 0.000501 0.000085 0.000400 0.001389 0.000494 0.003152
Golgi 0.004739 0.000460 0.000203 0.004343 0.000226 0.000692 0.000107 0.000024 0.000490 0.000572 0.000121 0.003642
Lipid Particles 0.000778 0.001061 0.000578 0.001082 0.000951 0.002117 0.000278 0.000064 0.000941 0.001573 0.000322 0.000917
Mitochondria 0.001958 0.004333 0.001126 0.007871 0.001151 0.005437 0.001259 0.000947 0.001198 0.004868 0.001240 0.006747
Mitotic Spindle 0.003006 0.007242 0.000986 0.043989 0.001072 0.009309 0.004989 0.003451 0.001185 0.008244 0.004402 0.037410
None 0.003664 0.016903 0.003525 0.016146 0.008288 0.016054 0.008039 0.007562 0.008018 0.016491 0.007378 0.014753
Nuclear Periphery 0.000254 0.002227 0.000414 0.002266 0.000641 0.001866 0.000787 0.000721 0.000618 0.002052 0.000733 0.002016
Nuclear Periphery Foci 0.000074 0.001984 0.000545 0.000907 0.000724 0.001600 0.000341 0.000010 0.000686 0.001798 0.000371 0.000762
Nucleolus 0.027028 0.028113 0.031785 0.002815 0.020181 0.028291 0.008453 0.000377 0.020581 0.028199 0.011874 0.002419
Nucleus 0.941455* 0.858706* 0.931032* 0.642650* 0.938444* 0.851787* 0.925650* 0.870636* 0.938620* 0.855351* 0.926439* 0.679653*
Peroxisomes 0.000752 0.001222 0.000827 0.001008 0.000567 0.001860 0.000157 0.000052 0.000578 0.001531 0.000255 0.000852
Vacuole 0.001492 0.002595 0.003909 0.025746 0.000924 0.003318 0.004006 0.001686 0.000957 0.002946 0.003992 0.021841
Vacuole Periphery 0.000065 0.000651 0.000199 0.003070 0.000140 0.000760 0.000136 0.000069 0.000136 0.000704 0.000145 0.002583

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.40 -7.01 -3.35 -2.26 5.72 -5.12 -15.11 6.03 8.52 22.19* -8.23 -17.18 -6.95 -1.76 3.89
Bud Neck -13.01 -7.87 -16.60 -7.48 -7.00 -8.41 -17.70 -5.19 -2.52 -0.60 -14.77 -19.02 -17.04 -9.03 -6.60
Bud Site -6.92 -5.72 -9.03 -7.39 -7.18 -3.95 -9.34 -2.46 1.21 0.65 -6.44 -10.18 -8.97 -7.18 -7.67
Cell Periphery -5.34 -3.78 -7.98 -4.66 -4.82 -2.05 -2.39 1.69 5.00 5.81 -3.49 -2.82 -6.52 -4.83 -5.34
Cytoplasm -6.63 1.47 -16.84 -12.90 -24.32 -8.36 -0.83 -8.79 -4.83 -8.56 -10.32 -0.10 -21.28 -14.02 -21.80
Cytoplasmic Foci 0.91 1.51 0.83 -0.49 -5.12 -3.24 4.81 5.16 7.45 1.92 -1.77 4.96 0.62 2.81 -5.81
Eisosomes -1.15 -6.05 -3.19 -5.88 5.05 -3.52 -5.73 9.07 13.87 19.88 -3.17 -8.01 -5.19 -2.68 3.61
Endoplasmic Reticulum -16.03 -11.78 -19.67 -5.17 3.12 -4.34 -13.35 8.98 10.71 21.44 -8.71 -15.82 -12.09 -4.70 2.86
Endosome -0.78 -0.29 -2.11 -2.50 -3.07 -4.28 -1.30 3.82 5.91 13.09 -5.04 -1.52 -4.74 -0.55 -4.36
Golgi 0.90 0.93 0.71 -1.14 -1.39 -2.56 1.25 2.67 4.23 13.36 -0.34 1.21 -0.88 -0.80 -1.56
Lipid Particles -0.68 -0.15 -0.16 1.21 -0.02 -2.50 4.33 5.44 5.74 4.28 -1.86 3.97 1.84 3.94 -2.49
Mitochondria -1.50 0.31 -1.67 -0.38 -5.45 -4.68 -1.14 -0.39 4.71 1.15 -6.27 -0.94 -4.93 1.00 -5.10
Mitotic Spindle -1.52 -0.39 -5.78 -5.75 -6.92 -4.24 -3.34 -1.35 1.72 0.49 -5.06 -3.38 -8.50 -5.09 -6.46
None -7.79 0.01 -5.27 4.11 -6.61 -4.81 1.62 -0.48 4.71 -2.41 -6.55 1.95 -1.76 5.47 -4.46
Nuclear Periphery -5.45 -3.47 -15.13 -4.76 -13.33 -3.56 -6.25 -2.34 1.64 -0.09 -5.64 -6.09 -13.87 -5.31 -11.93
Nuclear Periphery Foci -4.98 -2.12 -6.84 2.99 1.25 -1.97 1.57 4.98 5.68 5.10 -3.09 0.44 1.08 4.41 0.60
Nucleolus -0.45 -0.88 2.65 15.22 7.82 -3.26 6.52 13.13 16.70 11.51 -3.68 4.61 12.13 21.73 11.64
Nucleus 3.38 0.82 9.75 15.63 18.91 11.96 3.42 6.24 -1.12 4.82 14.63 3.68 25.96 14.85 24.86
Peroxisomes -0.89 -0.61 0.62 3.14 3.47 -3.80 3.44 4.47 6.01 4.07 -3.82 2.58 2.30 5.79 0.20
Vacuole 0.81 0.31 -3.94 -15.79 -12.13 -3.46 -7.21 -2.91 -0.08 3.13 -1.98 -5.82 -15.78 -15.46 -13.41
Vacuole Periphery -3.22 -8.30 -3.01 -1.02 -2.41 -5.84 0.24 3.27 6.63 3.71 -5.25 -0.88 -2.71 -0.55 -2.65
Standard name
Human Ortholog
Description Protein of unknown function; required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Gds1

Gds1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gds1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available