Standard name
Human Ortholog
Description Autoregulatory, oleate-activated transcription factor; subunit of a heterodimeric complex with Oaf1p, which binds to oleate-response elements (ORE) in the promoter of genes involved in beta-oxidation of fatty acids, peroxisome organization and biogenesis, activating transcription in the presence of oleate; PIP2 has a paralog, OAF1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.08 0.24 0.17 0.17 0.17 0.22 0.21 0.13 – 0.11 0.13 0.24 0.28 0.32 0.33 0.29 0.34 0.05 0 0.11 0.09 0.13 0.18
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.06 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.14 0.29 0.53 0.34 0.65 0.64 0.71 0.87 – 0.87 0.88 0.17 0.07 0.27 0.08 0 0.08 0 0 0.06 0 0.05 0.05
Nucleus 0.72 0.59 0.5 0.53 0.52 0.41 0.36 0.31 0.32 – 0.27 0.27 0.59 0.54 0.38 0.35 0.48 0.4 0.63 0.64 0.44 0.54 0.38 0.25
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Vac/Vac Membrane 0.15 0.27 0.06 0 0.07 0.06 0.06 0 0 – 0 0.06 0.1 0.13 0.16 0.27 0.18 0.17 0.17 0.16 0.28 0.25 0.29 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 1 2 0 1 0
Bud 0 0 6 3 3 3 5 9 8 – 14 7 0 0 2 1 0 0 3 6 4 5 7 9
Bud Neck 0 0 2 1 1 3 2 2 0 – 3 0 0 0 0 0 0 0 0 0 2 1 2 4
Bud Site 0 0 0 1 1 5 10 15 12 – 11 8 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 23 20 87 67 67 90 148 108 44 – 36 57 62 96 122 100 60 62 13 12 54 22 38 55
Endoplasmic Reticulum 2 3 0 0 0 0 1 1 0 – 2 0 0 1 0 17 10 11 0 0 0 0 1 3
Endosome 8 11 1 1 15 2 1 2 0 – 0 0 1 1 1 16 13 11 6 7 6 0 3 7
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 1 4 4 0 0 3 2
Mitochondria 4 34 105 203 131 348 438 371 292 – 287 395 43 25 103 25 7 14 6 8 28 2 14 14
Nucleus 162 147 178 204 201 219 244 162 107 – 90 121 153 183 148 107 98 74 175 198 209 134 117 77
Nuclear Periphery 1 1 1 0 0 0 0 1 0 – 2 0 0 0 0 0 0 0 1 1 0 0 0 1
Nucleolus 0 1 0 1 2 0 0 2 1 – 0 4 0 1 1 0 0 0 2 2 2 3 4 5
Peroxisomes 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 1 2 3 3 4 10 10 0 – 2 5 1 2 1 1 4 0 10 10 15 9 14 18
Vac/Vac Membrane 35 67 22 9 27 32 39 16 9 – 14 27 25 44 63 83 36 31 47 49 135 60 87 101
Unique Cell Count 226 249 356 385 383 534 683 521 336 331 451 259 337 387 304 205 183 279 309 479 247 305 311
Labelled Cell Count 236 285 404 493 452 706 898 699 473 462 624 285 353 443 352 229 205 279 309 479 247 305 311


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 4.6 3.7 3.5 3.7 3.3 3.3 3.1 2.8 – 2.7 2.7 4.3 4.4 3.8 5.4 6.0 5.6 4.6 4.6 5.2
Std Deviation (1e-4) 0.7 1.1 0.7 0.9 1.1 0.8 1.3 0.8 0.8 – 0.6 0.7 1.2 0.9 0.8 1.0 1.5 1.4 1.2 1.5 1.7
Intensity Change (Log2) – – – -0.08 0.02 -0.15 -0.14 -0.25 -0.41 – -0.42 -0.46 0.23 0.26 0.06 0.55 0.71 0.62 0.32 0.32 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 7.4 4.3 4.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.363 2.4963 1.9103 1.9808 1.716 2.172 2.2863 3.7314 3.257 3.0738 2.7908 3.2188 3.5998 4.2148 3.699 3.3663 3.6618 4.0547
Actin 0.016 0.0001 0.0045 0.0013 0.0026 0.0001 0.0005 0 0 0 0.0001 0 0.0001 0 0 0 0.0001 0.0001
Bud 0.0004 0 0.0002 0.0004 0.0002 0 0 0 0.0001 0 0 0 0 0.0006 0 0 0 0
Bud Neck 0.0105 0.0003 0.0017 0.0128 0.0016 0.002 0.0007 0.0001 0.0003 0.0003 0.0002 0.0006 0.0003 0.0002 0.0001 0.0009 0.0007 0.0014
Bud Periphery 0.0012 0 0.0006 0.0033 0.0006 0.0001 0.0001 0 0 0 0 0 0 0.0002 0 0 0 0
Bud Site 0.0018 0.0001 0.0014 0.0009 0.0001 0.0001 0.0002 0.0001 0.0001 0 0 0 0.0001 0.0002 0 0.0001 0 0
Cell Periphery 0.0004 0 0.0002 0.0013 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.0081 0.0056 0.0074 0.0005 0.0014 0.0009 0.0064 0.0043 0.0043 0.0006 0.0037 0.0039 0.0111 0.0086 0.0112 0.0093 0.0127 0.0055
Cytoplasmic Foci 0.0077 0.0001 0.0016 0.0002 0.0003 0.0001 0.0004 0.0001 0.0002 0.0003 0.0001 0.0003 0.0014 0.0002 0.0002 0.0005 0.0007 0.0008
Eisosomes 0.0004 0 0.0001 0.0001 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0028 0.0002 0.0013 0.0009 0.0006 0.0001 0.0004 0.0001 0.0001 0 0 0.0001 0.001 0.0009 0.0001 0.0001 0.0006 0.0002
Endosome 0.0128 0.0002 0.0051 0.0042 0.0023 0.0001 0.0009 0 0.0002 0.0001 0.0001 0.0002 0.0011 0.0014 0.0001 0.0002 0.0005 0.0004
Golgi 0.0041 0 0.0036 0.0008 0.0004 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Lipid Particles 0.0124 0 0.0023 0.0009 0.0007 0.0001 0.0001 0 0 0 0 0 0.0004 0.0002 0 0 0.0001 0.0001
Mitochondria 0.0099 0.0001 0.0043 0.0028 0.002 0.0002 0.0003 0 0.0001 0 0.0001 0.0001 0.0002 0.0003 0.0001 0.0001 0.0002 0.0001
None 0.0109 0.0068 0.0072 0.0528 0.0005 0.0004 0.014 0.0221 0.0077 0.0003 0.0729 0.0074 0.0229 0.0159 0.0239 0.0052 0.0411 0.0172
Nuclear Periphery 0.0264 0.0111 0.0092 0.0035 0.0198 0.0021 0.0041 0.002 0.003 0.0011 0.0023 0.0013 0.0055 0.0094 0.0029 0.0049 0.0187 0.002
Nucleolus 0.0178 0.0095 0.011 0.01 0.0219 0.0252 0.0098 0.0063 0.0061 0.0095 0.0093 0.013 0.0094 0.0055 0.0051 0.0079 0.0168 0.0154
Nucleus 0.8054 0.9425 0.9034 0.8533 0.8359 0.9415 0.9305 0.9498 0.949 0.9137 0.8821 0.9452 0.9243 0.9383 0.9345 0.8893 0.8652 0.9205
Peroxisomes 0.0078 0 0.0007 0.0002 0.0004 0.0001 0 0 0 0 0 0 0 0 0 0 0 0
Punctate Nuclear 0.037 0.0225 0.0321 0.041 0.1077 0.0266 0.0306 0.015 0.0286 0.0739 0.029 0.0278 0.0217 0.0167 0.0215 0.0813 0.042 0.0359
Vacuole 0.0038 0.0009 0.0012 0.0073 0.0006 0.0002 0.0006 0.0001 0.0001 0 0.0001 0.0001 0.0006 0.001 0.0001 0.0001 0.0003 0.0001
Vacuole Periphery 0.0028 0.0001 0.0007 0.0013 0.0007 0 0.0001 0 0.0001 0 0 0 0.0001 0.0003 0 0 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.6693 7.2552 5.8731 7.9544 6.9354 6.268 9.5864 10.5774 10.6315 9.3358
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
816 116 1936 1237 666 1369 1691 127 1482 1485 3627 1364

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 548.09 772.79 839.75 795.39 617.22 710.86 866.63 869.03 579.16 715.70 852.28 802.25
Standard Deviation 64.08 86.66 97.43 104.57 98.83 88.87 95.61 101.56 88.50 90.24 97.51 106.46
Intensity Change Log 2 — 0.495664 0.615547 0.537250 — 0.203781 0.489631 0.493621 — 0.348437 0.550225 0.514306

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000050 0.000457 0.000250 0.000551 0.000056 0.000334 0.000194 0.003000 0.000052 0.000343 0.000224 0.000779
Bud Neck 0.012844 0.005171 0.005713 0.010566 0.003911 0.007438 0.006424 0.006258 0.008830 0.007261 0.006045 0.010165
Bud Site 0.001631 0.001587 0.003422 0.017048 0.000726 0.003906 0.003561 0.064277 0.001224 0.003725 0.003487 0.021445
Cell Periphery 0.000195 0.000145 0.000082 0.000188 0.000209 0.000174 0.000086 0.000186 0.000201 0.000172 0.000084 0.000188
Cytoplasm 0.407868* 0.071032 0.023929 0.179242* 0.261523* 0.142007* 0.076176 0.125347* 0.342101* 0.136463* 0.048288 0.174224*
Cytoplasmic Foci 0.129755 0.049509 0.000432 0.006751 0.104352 0.031671 0.000684 0.014933 0.118339 0.033065 0.000549 0.007513
Eisosomes 0.000195 0.000081 0.000017 0.000034 0.000114 0.000043 0.000016 0.000041 0.000159 0.000046 0.000017 0.000034
Endoplasmic Reticulum 0.000576 0.003012 0.003867 0.004574 0.000623 0.002096 0.003507 0.006800 0.000597 0.002167 0.003699 0.004781
Endosome 0.004498 0.002702 0.000487 0.007193 0.002248 0.003136 0.000684 0.005929 0.003487 0.003103 0.000579 0.007076
Golgi 0.000647 0.000554 0.000391 0.003610 0.000519 0.001353 0.000060 0.016981 0.000589 0.001291 0.000237 0.004855
Lipid Particles 0.009013 0.007509 0.000301 0.000894 0.010518 0.005535 0.000328 0.001117 0.009689 0.005689 0.000313 0.000914
Mitochondria 0.003509 0.016007 0.001353 0.002801 0.003711 0.006332 0.001107 0.002877 0.003600 0.007088 0.001239 0.002808
Mitotic Spindle 0.000355 0.000338 0.002090 0.039976 0.000149 0.006288 0.003572 0.011462 0.000263 0.005823 0.002781 0.037321
None 0.023525 0.008565 0.003321 0.010142 0.021969 0.010733 0.005752 0.013483 0.022826 0.010564 0.004454 0.010453
Nuclear Periphery 0.000839 0.001068 0.001183 0.002520 0.001348 0.001477 0.001867 0.001884 0.001068 0.001445 0.001502 0.002461
Nuclear Periphery Foci 0.000348 0.000626 0.000131 0.001322 0.000330 0.000711 0.000220 0.000794 0.000340 0.000704 0.000172 0.001273
Nucleolus 0.003157 0.009161 0.001073 0.001632 0.004671 0.005703 0.000773 0.000539 0.003837 0.005973 0.000933 0.001531
Nucleus 0.378844* 0.805552* 0.947251* 0.674658* 0.571451* 0.756718* 0.884375* 0.677987* 0.465400* 0.760532* 0.917936* 0.674968*
Peroxisomes 0.003161 0.008271 0.000122 0.001094 0.003883 0.004081 0.000069 0.000391 0.003486 0.004408 0.000097 0.001029
Vacuole 0.018601 0.008123 0.004469 0.032226 0.007336 0.009305 0.010188 0.045199 0.013538 0.009213 0.007135 0.033434
Vacuole Periphery 0.000389 0.000531 0.000115 0.002977 0.000355 0.000960 0.000356 0.000517 0.000374 0.000927 0.000228 0.002748

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.09 -12.89 -14.02 -0.03 -7.41 -2.38 -10.76 -1.69 -1.47 -1.53 -2.81 -16.80 -5.91 -2.62 -4.04
Bud Neck 2.37 2.82 0.30 -2.92 -5.42 -3.21 -3.49 -4.21 -1.49 -1.67 0.77 1.34 -2.18 -3.56 -5.32
Bud Site -0.37 -2.20 -5.78 -5.31 -4.51 -3.34 -3.93 -2.27 -1.92 -1.97 -2.86 -4.15 -6.37 -4.49 -4.99
Cell Periphery 1.14 9.58 3.06 0.51 -6.72 0.77 5.76 0.68 -0.12 -2.55 0.69 10.04 3.29 0.48 -7.39
Cytoplasm 23.23 41.00 26.65 -4.45 -19.17 10.89 19.39 11.98 4.69 -1.12 23.76 41.91 26.29 2.18 -15.14
Cytoplasmic Foci 5.43 23.19 22.48 3.86 -4.85 11.42 17.38 15.05 7.34 -1.66 18.00 28.81 27.72 12.42 -4.92
Eisosomes 1.42 2.73 2.18 2.40 -14.48 4.65 6.24 1.31 -5.16 -7.88 3.06 3.86 2.80 -2.82 -16.73
Endoplasmic Reticulum -3.64 -15.56 -20.50 -6.14 -8.49 -8.96 -18.20 -8.87 -7.86 -6.21 -9.52 -23.33 -22.64 -16.82 -10.56
Endosome 1.59 6.22 1.34 -0.41 -4.36 -1.44 5.18 -1.14 -0.47 -2.66 0.81 7.66 0.31 -0.32 -4.61
Golgi -0.08 0.98 -0.91 -0.84 -1.41 -2.18 3.83 -0.89 -0.67 -1.02 -2.05 2.16 -1.34 -0.12 -2.02
Lipid Particles 0.01 9.20 8.41 3.25 -9.55 3.56 9.21 7.61 4.06 -4.76 3.52 13.01 11.90 5.71 -11.06
Mitochondria -1.27 2.47 1.72 1.44 -1.63 -2.18 3.12 2.11 4.49 -1.15 -2.77 3.87 2.55 4.48 -2.20
Mitotic Spindle -0.72 -2.91 -8.00 -7.82 -7.25 -4.36 -3.83 -2.54 -2.03 -2.25 -4.30 -4.77 -8.41 -6.39 -7.51
None 4.21 6.56 4.82 0.17 -5.44 3.50 5.57 4.49 2.36 -1.34 5.18 8.59 6.43 2.39 -4.88
Nuclear Periphery -0.63 -3.13 -13.28 -7.51 -13.82 -0.37 -3.21 -5.08 -5.45 -3.89 -1.79 -4.51 -13.13 -13.10 -12.66
Nuclear Periphery Foci -1.25 3.35 -6.03 -0.31 -7.11 -3.13 0.14 -4.33 -3.28 -4.35 -3.37 2.32 -7.03 -3.48 -7.65
Nucleolus -2.87 5.13 4.61 3.88 -0.60 -1.00 4.72 4.74 7.11 0.32 -2.69 6.74 5.96 7.18 -1.34
Nucleus -15.19 -55.11 -25.22 3.32 27.46 -12.73 -23.95 -2.63 3.58 8.14 -26.37 -52.86 -20.67 5.23 25.68
Peroxisomes -2.00 6.40 5.50 2.81 -2.74 0.25 6.15 5.77 6.35 -3.09 -1.20 8.82 7.68 6.66 -3.12
Vacuole 3.78 7.34 -12.97 -14.57 -16.41 -1.86 -5.96 -7.20 -7.06 -6.63 2.28 1.15 -15.92 -16.56 -16.35
Vacuole Periphery -1.07 5.40 -1.65 -1.15 -2.42 -3.14 0.53 0.36 3.09 -0.17 -3.22 2.68 -1.67 0.02 -2.20
Standard name
Human Ortholog
Description Autoregulatory, oleate-activated transcription factor; subunit of a heterodimeric complex with Oaf1p, which binds to oleate-response elements (ORE) in the promoter of genes involved in beta-oxidation of fatty acids, peroxisome organization and biogenesis, activating transcription in the presence of oleate; PIP2 has a paralog, OAF1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (34%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Pip2

Pip2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pip2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available