Standard name
Human Ortholog
Description Mitochondrial aldehyde dehydrogenase; required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed; can substitute for cytosolic NADP-dependent aldehyde dehydrogenase when directed to the cytosol; human homolog ALDH2 can complement yeast ald4 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.13 0.09 0.06 0.09
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.08 0.17 0.31 0.17 0.05 0.08 0.06 0.1 0.09 0.09
Mitochondria 0.92 0.81 0.88 0.94 0.89 0.92 0.93 0.9 0.95 0.98 0.94 0.95 0.82 0.84 0.78 0.25 0.21 0.08 0.74 0.71 0.62 0.57 0.58 0.57
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 0.1 0.08 0.1 0.09 0.19 0.21 0.23 0.26 0.22 0.19 0.23 0 0.05 0.07 0.43 0.3 0.44 0 0 0 0 0 0
Peroxisomes 0 0 0.08 0 0 0 0 0 0 0 0 0 0.05 0.09 0.12 0.07 0 0.06 0.07 0.05 0.14 0.13 0.1 0.09
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.11 0 0 0 0.06 0.06 0 0 0 0 0 0.05 0 0.05 0.09 0.15 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 3 2 1 1 0 0 1 2 0 1 8 7 6 3 2 0 13 19 27 13 13 19
Bud 5 0 2 5 1 0 0 3 1 1 3 4 7 5 3 0 0 0 2 5 0 1 2 4
Bud Neck 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 2 0
Bud Site 0 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 9 1 1 0 1 0 0 0 0 0 0 0 1 1 2 3 0 0 0 0 0 0 0
Cytoplasm 1 4 1 1 2 6 5 7 3 2 1 0 5 2 8 11 5 5 0 0 0 0 0 0
Endoplasmic Reticulum 1 2 1 0 0 1 0 0 0 1 0 0 2 2 0 7 5 2 0 2 0 0 0 1
Endosome 1 2 0 0 0 0 0 0 0 0 0 0 1 3 1 2 2 0 5 6 1 2 5 7
Golgi 1 8 4 4 5 1 1 0 2 0 5 4 13 15 18 34 30 6 14 32 12 15 20 19
Mitochondria 259 252 153 143 110 184 266 282 209 292 212 251 178 221 186 49 21 3 222 278 132 86 131 119
Nucleus 1 1 1 1 0 1 0 2 0 1 0 1 0 0 0 4 1 0 0 0 0 0 0 0
Nuclear Periphery 4 1 0 1 2 0 2 1 0 1 2 1 0 1 0 1 0 0 1 1 0 0 2 0
Nucleolus 27 31 14 15 11 38 61 73 57 66 43 60 8 14 16 84 29 16 2 6 1 3 8 2
Peroxisomes 12 12 14 6 3 2 1 3 0 0 1 0 11 23 29 14 4 2 22 17 29 19 23 18
SpindlePole 1 1 1 1 0 0 1 0 0 0 0 0 0 1 0 7 1 0 4 4 1 1 4 5
Vac/Vac Membrane 13 34 2 0 5 11 16 14 6 4 8 7 11 9 11 17 15 5 3 11 0 2 5 5
Unique Cell Count 282 312 173 152 123 199 287 315 220 298 225 263 218 262 237 195 98 36 299 392 212 152 225 209
Labelled Cell Count 330 358 198 180 140 247 353 385 279 370 275 330 244 304 279 235 118 39 299 392 212 152 225 209


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.6 25.3 31.2 31.7 37.9 41.7 43.2 46.1 50.8 50.2 50.8 51.0 30.4 31.1 30.7 56.8 62.5 57.4 17.0 20.6 20.0
Std Deviation (1e-4) 8.2 12.7 14.2 15.4 15.3 18.0 18.4 22.5 22.4 24.2 23.4 24.1 11.3 11.4 13.1 29.9 29.3 32.6 6.8 11.3 7.6
Intensity Change (Log2) – – – 0.02 0.28 0.42 0.47 0.56 0.7 0.68 0.7 0.71 -0.04 -0.01 -0.02 0.86 1.0 0.88 -0.88 -0.6 -0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 2.7 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 1.8 1.7 2.3 4.8 6.8 0
Mitochondria 1.8 0.3 1.3 1.5 0.4 2.4 4.4 2.1 2.7 -1.8 -1.2 -2.6 -12.2 -11.1 -10.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.6 0.3 3.1 3.7 4.2 4.6 3.9 3.1 4.0 -1.9 -1.1 -0.5 7.6 4.7 5.7
Peroxisomes -1.6 -2.1 -3.4 -4.5 -4.1 -4.3 -5.0 -4.0 -4.7 -1.2 0.3 1.4 -0.3 -1.3 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.3 2.4 2.0 0 0 0 0 2.1 0 2.0 3.3 4.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0011 0.0124 0.0069 0.0034 0.0001 0.0004 0.0029 0.0008 0.0038 0.003 0.007 0 – – – – – –
Bud 0.0001 0.0001 0.0014 0 0 0 0.0003 0.0004 0.0002 0 0.0001 0 – – – – – –
Bud Neck 0.0001 0.0001 0.0001 0 0 0 0.0001 0.0001 0 0 0.0001 0 – – – – – –
Bud Periphery 0.0001 0.0001 0.001 0 0 0 0.0005 0.0002 0.0004 0 0.0002 0 – – – – – –
Bud Site 0.0007 0.0004 0.0022 0 0 0 0.0004 0.0004 0.0002 0 0.0004 0 – – – – – –
Cell Periphery 0.0001 0.0003 0.0017 0.0001 0 0 0.0026 0.0005 0.0003 0 0 0.0001 – – – – – –
Cytoplasm 0.0001 0 0.0001 0 0 0 0.0001 0 0 0 0 0 – – – – – –
Cytoplasmic Foci 0.0248 0.0039 0.0292 0.0003 0.0001 0 0.0025 0.0194 0.0035 0.0006 0.0027 0.0138 – – – – – –
Eisosomes 0.0006 0.0112 0.0012 0.0013 0.0001 0.0003 0.0012 0.0023 0.0034 0.0004 0.0005 0.0001 – – – – – –
Endoplasmic Reticulum 0 0 0.0001 0 0 0 0 0 0 0 0 0 – – – – – –
Endosome 0.0001 0 0.0015 0.0001 0 0 0.0002 0 0.0003 0 0.0005 0 – – – – – –
Golgi 0.0002 0.0001 0.0026 0.0004 0 0.0001 0.0007 0.0001 0.0033 0.0003 0.0021 0.0002 – – – – – –
Lipid Particles 0.008 0.0009 0.0132 0.0025 0.0001 0.0016 0.0204 0.0029 0.0196 0.0126 0.0053 0.0073 – – – – – –
Mitochondria 0.8808 0.9561 0.8734 0.9811 0.9801 0.9962 0.9123 0.9418 0.9247 0.9556 0.9293 0.9665 – – – – – –
None 0.001 0.0001 0.0004 0 0 0 0.0001 0.0001 0.0003 0 0 0 – – – – – –
Nuclear Periphery 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 – – – – – –
Nucleolus 0.0385 0.0046 0.0227 0.0001 0 0 0.0267 0.0198 0.0049 0.0001 0 0.0004 – – – – – –
Nucleus 0.0023 0.0001 0.0014 0 0 0 0.0009 0.0014 0.0006 0 0 0 – – – – – –
Peroxisomes 0.0358 0.008 0.0322 0.01 0.0195 0.0006 0.0149 0.0051 0.0195 0.0272 0.0506 0.0016 – – – – – –
Punctate Nuclear 0.0038 0 0.0003 0 0 0 0 0 0 0 0 0 – – – – – –
Vacuole 0.0004 0.0004 0.0037 0.0001 0 0 0.0052 0.001 0.0016 0 0.0001 0.0005 – – – – – –
Vacuole Periphery 0.0015 0.0011 0.0045 0.0006 0 0.0006 0.0079 0.0038 0.013 0.0001 0.001 0.0093 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 102.9137 34.7761 39.4118 81.0524 39.4335 153.4014 101.97 117.5814 164.9846 69.0036
Translational Efficiency 1.6167 1.8261 1.58 1.7008 1.4138 1.8362 1.4757 1.6986 1.6306 1.5671

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
155 112 253 1790 1774 1640 200 1010 1929 1752 453 2800

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2015.99 1463.31 6195.65 3662.63 1312.21 1545.46 5027.94 3507.90 1368.76 1540.21 5680.10 3606.82
Standard Deviation 785.22 511.83 1891.18 1309.93 498.40 548.28 1851.74 1788.72 560.88 546.40 1961.53 1502.19
Intensity Change Log 2 — -0.462253 1.619767 0.861391 — 0.236038 1.937969 1.418609 — -0.145568 1.753720 1.107338

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.008944 0.011116 0.013151 0.023273 0.003900 0.008054 0.020396 0.016864 0.004305 0.008249 0.016350 0.020961
Bud Neck 0.006761 0.012325 0.028221 0.007297 0.001575 0.003700 0.017293 0.034951 0.001992 0.004251 0.023396 0.017272
Bud Site 0.005261 0.011466 0.013532 0.017959 0.002607 0.005826 0.008305 0.139642* 0.002820 0.006187 0.011225 0.061852
Cell Periphery 0.000170 0.000723 0.000439 0.000291 0.000099 0.000187 0.000203 0.002868 0.000105 0.000221 0.000335 0.001221
Cytoplasm 0.001273 0.001358 0.000688 0.003408 0.000949 0.001274 0.001098 0.026504 0.000975 0.001279 0.000869 0.011739
Cytoplasmic Foci 0.099784 0.071809 0.179391 0.068555 0.022649 0.052469 0.204061* 0.106154 0.028847 0.053706 0.190283* 0.082117
Eisosomes 0.002090 0.005514 0.003690 0.002322 0.000660 0.001094 0.003778 0.001532 0.000775 0.001377 0.003729 0.002037
Endoplasmic Reticulum 0.000400 0.000381 0.000355 0.000221 0.000152 0.000221 0.000236 0.000712 0.000172 0.000232 0.000302 0.000398
Endosome 0.008709 0.004404 0.002646 0.002294 0.004974 0.006481 0.002865 0.007193 0.005275 0.006348 0.002743 0.004061
Golgi 0.034301 0.025883 0.018549 0.019495 0.018808 0.030837 0.026006 0.011811 0.020053 0.030520 0.021841 0.016723
Lipid Particles 0.023246 0.010185 0.036160 0.009645 0.009302 0.016868 0.019075 0.004873 0.010422 0.016441 0.028617 0.007924
Mitochondria 0.693487* 0.749403* 0.498946* 0.739153* 0.903268* 0.819430* 0.540148* 0.489047* 0.886412* 0.814953* 0.517137* 0.648936*
Mitotic Spindle 0.021225 0.003195 0.050295 0.009702 0.002688 0.003409 0.032091 0.015838 0.004178 0.003396 0.042258 0.011916
None 0.003426 0.003720 0.005901 0.001759 0.001822 0.002109 0.003089 0.002653 0.001951 0.002212 0.004660 0.002082
Nuclear Periphery 0.002608 0.000339 0.011861 0.000432 0.000671 0.000737 0.003596 0.000211 0.000826 0.000712 0.008212 0.000352
Nuclear Periphery Foci 0.001836 0.000568 0.003407 0.000226 0.000671 0.000596 0.001441 0.000568 0.000765 0.000594 0.002539 0.000349
Nucleolus 0.006146 0.008103 0.027684 0.002814 0.000835 0.002077 0.006689 0.005814 0.001262 0.002462 0.018415 0.003896
Nucleus 0.000584 0.000976 0.001476 0.000554 0.000181 0.000527 0.000321 0.005296 0.000213 0.000556 0.000966 0.002265
Peroxisomes 0.030087 0.065098 0.027415 0.080357 0.016195 0.030003 0.072472 0.102733* 0.017311 0.032247 0.047308 0.088428
Vacuole 0.026545 0.009088 0.037327 0.004728 0.004084 0.007820 0.018105 0.016726 0.005889 0.007901 0.028841 0.009056
Vacuole Periphery 0.023117 0.004347 0.038864 0.005513 0.003910 0.006281 0.018730 0.008010 0.005453 0.006157 0.029975 0.006414

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.61 -1.15 -5.34 -3.89 -3.03 -3.57 -3.41 -6.27 -4.07 0.69 -3.55 -4.36 -12.34 -8.61 -1.56
Bud Neck -1.51 -7.04 -0.41 1.44 7.52 -5.06 -6.02 -16.24 -15.08 -5.40 -4.87 -10.99 -17.78 -14.22 2.91
Bud Site -1.32 -2.75 -5.99 -1.29 -1.33 -3.24 -4.80 -20.57 -19.99 -19.54* -3.45 -5.13 -20.81 -19.10 -15.66
Cell Periphery -1.24 -4.24 -3.52 0.96 2.28 -3.36 -4.26 -8.11 -7.84 -7.80 -3.08 -6.28 -8.81 -7.63 -6.77
Cytoplasm -0.29 1.41 -3.38 -3.18 -5.47 -0.89 -0.35 -11.89 -11.83 -11.95 -0.91 0.45 -11.95 -12.02 -12.71
Cytoplasmic Foci 1.51 -4.35 2.32 0.29 8.02 -8.36 -10.99 -13.60 -8.19 5.53 -6.94 -15.27 -14.54 -6.74 9.91
Eisosomes -2.19 -2.80 -0.69 2.07 2.70 -4.04 -6.26 -3.65 -1.79 4.16 -4.17 -8.35 -8.84 -3.69 4.60
Endoplasmic Reticulum 0.18 0.44 2.15 2.12 1.90 -2.25 -1.82 -7.34 -6.62 -5.85 -2.00 -2.72 -5.89 -4.83 -1.86
Endosome 1.37 1.99 2.14 1.80 0.62 -1.68 2.67 -2.76 -0.82 -6.07 -1.21 3.50 1.90 3.50 -2.95
Golgi 0.71 1.81 1.76 0.74 -0.33 -4.48 -1.86 3.39 7.73 3.83 -3.97 -0.65 1.74 6.07 2.17
Lipid Particles 2.36 -1.87 2.66 0.20 5.53 -4.22 -3.06 3.40 7.17 4.55 -3.49 -5.81 1.98 5.43 6.79
Mitochondria -1.35 5.48 -1.61 0.30 -10.28 9.64 14.10 31.32* 23.56 1.94 8.19 21.13 27.10 17.12 -7.24
Mitotic Spindle 2.25 -2.30 1.53 -2.93 4.71 -0.67 -3.80 -6.94 -6.56 2.05 0.69 -6.37 -5.72 -7.37 5.10
None -0.36 -3.82 4.61 2.58 7.44 -1.47 -4.07 -1.96 -1.33 0.83 -1.38 -7.46 -0.64 0.59 6.91
Nuclear Periphery 1.63 -3.70 1.57 -0.98 5.50 -0.26 -2.77 3.22 2.43 3.24 0.43 -5.80 2.74 1.77 6.22
Nuclear Periphery Foci 2.90 -2.25 3.76 2.83 6.38 0.39 -2.44 0.54 0.20 3.07 0.99 -5.17 2.67 2.63 7.11
Nucleolus -0.38 -4.27 1.56 1.09 5.36 -3.46 -6.27 -5.08 -3.71 0.60 -2.49 -6.43 -5.20 -2.36 5.38
Nucleus -0.90 -2.80 0.04 0.93 2.97 -1.57 -1.92 -5.36 -4.85 -5.24 -1.66 -4.57 -5.67 -4.02 -3.61
Peroxisomes -2.73 0.41 -7.47 -1.27 -9.88 -5.01 -6.52 -14.49 -11.74 -2.88 -5.50 -6.41 -19.98 -14.33 -7.49
Vacuole 2.85 -1.63 4.06 1.72 7.95 -3.94 -6.42 -8.08 -5.41 0.50 -2.03 -8.87 -3.53 -1.24 7.68
Vacuole Periphery 3.20 -2.11 3.02 -0.96 7.05 -3.64 -3.47 -3.94 -1.58 2.46 -0.91 -7.42 -1.20 -0.36 7.15
Standard name
Human Ortholog
Description Mitochondrial aldehyde dehydrogenase; required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed; can substitute for cytosolic NADP-dependent aldehyde dehydrogenase when directed to the cytosol; human homolog ALDH2 can complement yeast ald4 mutant
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Ald4

Ald4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ald4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available