Standard name
Human Ortholog
Description Cytoplasmic protein of unknown function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.1 0.1 0.1 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.96 0.95 0.97 0.93 0.92 – 0.86 0.88 0.84 0.83 0.99 0.99 0.97 0.89 0.94 0.93 0.96 0.96 0.91 0.97 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.09 0 0.22 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 0 0 0 3 1 – 2 1 0 0 0 1 0 0 0 0 0 0 0 0 0 2
Bud 3 0 0 1 0 1 2 – 6 1 0 3 1 0 0 0 0 0 2 1 1 0 0 3
Bud Neck 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 1 1 0 0 1 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 16 12 11 3 12 16 10 – 13 11 6 10 17 23 28 60 62 38 0 0 0 0 0 0
Cytoplasm 974 393 354 241 381 391 401 – 381 386 213 260 436 661 478 534 557 356 975 379 338 246 427 604
Endoplasmic Reticulum 11 5 6 1 10 8 15 – 5 15 6 7 3 4 8 21 20 12 17 2 13 1 4 10
Endosome 2 0 0 0 0 0 0 – 0 1 0 1 0 1 2 1 0 0 1 0 1 0 0 2
Golgi 1 0 0 0 0 0 1 – 0 0 0 0 0 0 0 3 2 0 3 1 0 0 0 0
Mitochondria 4 0 0 4 1 13 18 – 41 19 56 46 1 0 0 8 3 1 0 1 1 1 0 2
Nucleus 3 0 5 4 1 1 4 – 0 4 1 5 4 1 6 5 4 2 1 0 2 1 2 0
Nuclear Periphery 0 0 0 0 0 1 0 – 0 0 0 0 0 0 2 0 0 0 2 1 1 0 0 2
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 1 7 4 0 8 3 – 21 17 0 5 0 2 0 46 9 20 1 3 7 1 2 5
Unique Cell Count 994 399 368 254 391 420 435 441 438 254 313 440 670 493 600 591 381 1016 396 373 254 442 641
Labelled Cell Count 1019 411 383 258 405 442 455 469 456 282 337 462 694 524 678 657 429 1016 396 373 254 442 641


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.1 18.3 14.3 12.6 11.1 9.4 8.7 – 7.2 7.3 6.2 6.5 14.6 14.4 13.7 22.3 24.7 22.6 13.5 14.1 14.7
Std Deviation (1e-4) 2.3 3.4 2.3 2.1 1.6 1.5 1.5 – 1.4 1.2 1.2 1.0 2.5 2.3 2.2 5.6 6.0 5.3 2.3 2.4 2.7
Intensity Change (Log2) – – – -0.18 -0.36 -0.61 -0.71 – -1.0 -0.96 -1.22 -1.13 0.03 0.01 -0.06 0.64 0.79 0.66 -0.08 -0.02 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.5 0.1 0.6 -0.6 – 0 -0.4 -0.5 0.2 0.7 0.4 1.9 4.1 4.3 3.9
Cytoplasm -0.8 1.0 -1.9 -2.4 – -4.8 -4.2 -5.3 -5.7 2.8 2.6 0.6 -3.9 -1.3 -1.7
Endoplasmic Reticulum 0 0.9 0.3 1.6 – -0.6 1.6 0 0.6 0 0 0 1.7 1.6 1.4
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.4 3.9 – 6.0 4.0 9.4 7.6 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 2.2 1.6 0 -0.3 0 0 0 3.8 -0.4 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.1031 8.6483 7.5368 8.3715 7.8097 7.1399 6.7014 8.918 8.6734 6.9449 7.8 8.515 8.8618 11.4493 10.5722 10.5609 9.7454 10.3298
Actin 0.0658 0.0023 0.0232 0.0001 0.0004 0.0012 0.0363 0.0007 0.0332 0.0357 0.0016 0.0038 0.0066 0.0011 0.0008 0.0005 0 0.0004
Bud 0.0008 0.0005 0.0004 0.0003 0.0002 0.0001 0.0011 0.0002 0.0013 0.0012 0.0008 0.001 0.0001 0.0096 0.0011 0.0004 0.0001 0.0001
Bud Neck 0.0013 0.0002 0.0005 0.0003 0.0004 0.0007 0.0033 0.0001 0.002 0.0055 0.0028 0.0015 0.0004 0.0002 0.0003 0.0005 0.0003 0.0006
Bud Periphery 0.0008 0.0006 0.0002 0 0 0.0001 0.0012 0.0001 0.0013 0.002 0.0006 0.0014 0 0.0024 0.0002 0.0001 0 0
Bud Site 0.004 0.0057 0.0012 0.0001 0.0001 0.0001 0.0048 0.0007 0.0082 0.0017 0.0026 0.0002 0.0005 0.0018 0.001 0.0002 0 0.0001
Cell Periphery 0.0003 0.0002 0.0001 0 0 0 0.0005 0.0001 0.0004 0.0007 0.0003 0.0001 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0.6643 0.9564 0.893 0.9781 0.976 0.8705 0.766 0.9761 0.8736 0.649 0.8723 0.9023 0.9512 0.9692 0.952 0.9528 0.933 0.9387
Cytoplasmic Foci 0.0423 0.0036 0.0225 0.0058 0.005 0.0202 0.02 0.0049 0.0109 0.0289 0.0209 0.0124 0.0078 0.0036 0.0071 0.0113 0.0077 0.0087
Eisosomes 0.0008 0.0001 0.0001 0 0 0 0.0003 0 0.0003 0.0003 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0239 0.0036 0.0045 0.0015 0.005 0.0044 0.0345 0.0028 0.0163 0.0127 0.0035 0.0267 0.0083 0.0039 0.0183 0.0021 0.0087 0.021
Endosome 0.054 0.0067 0.021 0.0081 0.0074 0.0321 0.0377 0.0031 0.0195 0.0847 0.0471 0.0312 0.009 0.0032 0.0081 0.0158 0.0427 0.021
Golgi 0.0197 0.0023 0.0058 0.0004 0.0006 0.0111 0.0071 0.0007 0.0061 0.0113 0.0199 0.0035 0.0007 0.0004 0.0005 0.0003 0.0007 0.0015
Lipid Particles 0.0216 0.0005 0.0017 0 0 0.0073 0.0118 0.0006 0.0014 0.0594 0.0006 0.0009 0.0009 0.0001 0.0001 0 0 0
Mitochondria 0.0084 0.0128 0.005 0.0001 0.0004 0.0247 0.0049 0.0002 0.0066 0.008 0.0153 0.0033 0.0002 0.0004 0.0003 0.0003 0.0009 0.0011
None 0.0299 0.0008 0.0012 0.0004 0.0005 0.0039 0.0086 0.0032 0.0025 0.006 0.0017 0.001 0.0061 0.001 0.0024 0.0021 0.0007 0.0007
Nuclear Periphery 0.0153 0.0006 0.002 0.0007 0.0009 0.0013 0.0157 0.0006 0.0061 0.0204 0.0008 0.0031 0.0013 0.0006 0.0009 0.0025 0.001 0.0016
Nucleolus 0.0009 0.0001 0.0001 0 0 0 0.0009 0 0.0002 0.0019 0.0001 0 0 0 0 0.0001 0 0
Nucleus 0.0085 0.0007 0.0014 0.0012 0.0012 0.0014 0.018 0.0012 0.0025 0.0098 0.0016 0.0023 0.0016 0.0012 0.0014 0.0067 0.0008 0.0017
Peroxisomes 0.0189 0.0003 0.0061 0 0 0.0167 0.0045 0.0025 0.0013 0.0126 0.0011 0.0006 0.0005 0.0001 0.0001 0.0001 0 0.0001
Punctate Nuclear 0.0063 0.0001 0.0005 0.0001 0.0001 0.0009 0.0079 0.0003 0.0012 0.013 0.0002 0.0002 0.0013 0.0001 0.0005 0.0012 0 0.0001
Vacuole 0.0099 0.0015 0.0072 0.0022 0.0013 0.0023 0.0122 0.0015 0.0039 0.0263 0.0047 0.0035 0.0027 0.001 0.0039 0.0025 0.0017 0.0017
Vacuole Periphery 0.0022 0.0004 0.0025 0.0008 0.0004 0.001 0.003 0.0002 0.0012 0.0091 0.0016 0.0011 0.0004 0.0002 0.0007 0.0006 0.0016 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.5907 47.4025 44.9039 45.7139 60.7059 45.104 39.7706 38.371 36.3761 45.0465
Translational Efficiency 1.1004 1.1039 1.0432 1.0604 0.7735 1.0037 1.0339 0.8345 0.9382 0.9495

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
815 1453 341 1269 366 1463 2609 2833 1181 2916 2950 4102

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 614.51 1303.32 1624.37 1535.60 1319.17 1234.94 1623.17 1587.60 832.89 1269.01 1623.31 1571.51
Standard Deviation 114.68 226.86 237.25 251.48 167.62 203.99 241.25 246.23 352.10 218.38 240.79 249.03
Intensity Change Log 2 — 1.084683 1.402372 1.321294 — -0.095190 0.299184 0.267217 — 0.392491 0.747998 0.691676

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000131 0.000278 0.000750 0.000644 0.000666 0.000150 0.000150 0.000161 0.000297 0.000214 0.000219 0.000310
Bud Neck 0.013544 0.000352 0.002720 0.001588 0.000659 0.000499 0.000789 0.001472 0.009551 0.000426 0.001012 0.001508
Bud Site 0.027852 0.014426 0.021112 0.026642 0.020050 0.013238 0.014139 0.010218 0.025434 0.013830 0.014945 0.015299
Cell Periphery 0.000620 0.000149 0.001193 0.001956 0.000440 0.000105 0.000843 0.000155 0.000564 0.000127 0.000883 0.000712
Cytoplasm 0.585928* 0.902780* 0.834645* 0.841593* 0.771922* 0.863449* 0.853181* 0.667554* 0.643569* 0.883047* 0.851038* 0.721395*
Cytoplasmic Foci 0.226511* 0.042467 0.038111 0.015995 0.131162 0.056949 0.013581 0.015660 0.196962* 0.049733 0.016417 0.015764
Eisosomes 0.000406 0.000002 0.000006 0.000003 0.000007 0.000003 0.000002 0.000002 0.000282 0.000003 0.000003 0.000003
Endoplasmic Reticulum 0.001988 0.001534 0.001289 0.000327 0.009261 0.001503 0.000487 0.000447 0.004242 0.001518 0.000579 0.000410
Endosome 0.020890 0.002548 0.004845 0.003446 0.007829 0.004678 0.002537 0.005506 0.016842 0.003617 0.002804 0.004868
Golgi 0.015770 0.007103 0.005702 0.009485 0.009628 0.007062 0.005572 0.004457 0.013867 0.007082 0.005587 0.006012
Lipid Particles 0.005429 0.000606 0.009001 0.006428 0.001655 0.000498 0.003792 0.000826 0.004259 0.000551 0.004394 0.002559
Mitochondria 0.004946 0.000943 0.000021 0.000225 0.000054 0.000618 0.000175 0.001228 0.003430 0.000780 0.000157 0.000918
Mitotic Spindle 0.000553 0.002017 0.001698 0.001100 0.000773 0.001344 0.000588 0.001637 0.000621 0.001679 0.000717 0.001470
None 0.019828 0.000651 0.000700 0.000210 0.000468 0.002019 0.000109 0.000441 0.013828 0.001337 0.000177 0.000369
Nuclear Periphery 0.000111 0.000048 0.000064 0.000027 0.000034 0.000040 0.000020 0.000092 0.000087 0.000044 0.000025 0.000072
Nuclear Periphery Foci 0.000711 0.001244 0.005736 0.001069 0.001384 0.000522 0.001045 0.000561 0.000920 0.000882 0.001588 0.000718
Nucleolus 0.000805 0.000044 0.000052 0.000171 0.000039 0.000103 0.000108 0.000362 0.000568 0.000073 0.000101 0.000303
Nucleus 0.018444 0.003489 0.010284 0.003833 0.003551 0.005273 0.002200 0.008436 0.013829 0.004384 0.003135 0.007012
Peroxisomes 0.002202 0.000208 0.000202 0.000622 0.000390 0.000438 0.000270 0.000568 0.001641 0.000323 0.000262 0.000585
Vacuole 0.042294 0.013966 0.059964 0.077433 0.033278 0.037031 0.088796 0.262968* 0.039500 0.025538 0.085463 0.205571*
Vacuole Periphery 0.011035 0.005144 0.001908 0.007202 0.006750 0.004479 0.011615 0.017249 0.009707 0.004810 0.010493 0.014141

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.57 -7.04 -1.98 -1.37 0.83 3.69 3.63 3.38 -0.09 0.07 1.54 1.15 -0.10 -0.97 -0.70
Bud Neck 8.50 6.27 7.53 -8.63 3.85 1.74 -2.16 -7.38 -9.14 -6.21 8.40 7.72 7.22 -12.82 -4.56
Bud Site 5.65 1.66 0.56 -6.05 -1.31 3.04 2.65 4.92 2.73 4.82 6.65 5.85 6.16 -1.18 0.43
Cell Periphery 3.52 -2.93 -4.16 -6.55 -2.29 2.65 -2.66 2.30 -1.47 8.27 4.37 -2.52 -0.75 -6.53 1.97
Cytoplasm -30.96 -16.07 -22.66 9.44 -1.40 -6.72 -6.02 7.90 25.25 26.93 -27.82 -23.33 -8.19 29.14 22.19
Cytoplasmic Foci 25.11 24.54 29.87 11.03 8.66 8.79 14.58 14.33 15.06 -1.75 25.23 32.06 32.33 18.57 1.48
Eisosomes 3.37 3.34 3.36 -3.21 4.15 3.83 4.71 4.51 1.77 -0.06 3.38 3.38 3.38 0.11 0.36
Endoplasmic Reticulum 1.01 1.60 5.10 3.94 2.65 2.62 2.97 3.00 3.42 0.73 2.81 3.86 4.07 5.08 2.23
Endosome 13.65 10.80 12.11 -1.13 2.18 3.06 5.73 2.56 -1.14 -7.19 13.17 14.26 12.02 -2.66 -5.19
Golgi 6.93 9.12 3.99 -1.03 -2.16 2.41 5.21 6.64 2.97 2.25 7.36 10.08 8.97 1.92 0.08
Lipid Particles 5.07 -2.87 -0.72 -6.01 2.17 3.84 -5.30 2.53 -5.43 9.44 5.58 -0.48 2.28 -6.68 4.65
Mitochondria 5.06 7.89 7.55 1.49 -2.91 -2.80 -4.12 -4.87 -1.73 -4.32 5.21 7.51 5.57 -0.24 -4.44
Mitotic Spindle -2.96 -5.49 -1.80 1.14 1.50 -1.74 -0.14 -2.10 -0.26 -2.02 -3.53 -1.57 -3.02 0.40 -1.82
None 5.62 5.60 5.75 3.63 1.64 -2.33 2.57 0.25 2.41 -2.38 5.23 5.77 5.69 2.85 -1.91
Nuclear Periphery 2.33 1.08 3.86 0.89 4.24 -0.37 0.83 -3.31 -3.02 -4.90 2.36 3.90 0.37 -2.30 -4.39
Nuclear Periphery Foci -1.54 -9.68 -2.30 0.29 9.09 6.58 1.71 3.33 -0.25 2.21 0.21 -4.64 0.92 0.61 4.92
Nucleolus 6.71 6.62 5.25 -3.63 -3.43 -2.46 -5.07 -14.44 -6.77 -13.67 6.02 5.94 3.16 -9.44 -12.05
Nucleus 6.91 2.42 6.30 -3.31 5.61 -1.38 0.98 -4.73 -6.15 -13.98 6.01 6.63 3.60 -8.23 -11.17
Peroxisomes 4.86 4.77 3.84 -5.76 -5.09 -0.91 1.96 -2.04 0.14 -5.27 4.14 4.82 3.74 -1.61 -7.00
Vacuole 9.98 -3.57 -9.08 -17.11 -2.11 -0.81 -10.75 -34.24 -38.92 -29.67 4.98 -13.75 -37.42 -43.28 -25.32
Vacuole Periphery 4.10 7.57 3.09 -1.39 -5.91 1.26 -2.55 -4.87 -12.25 -4.31 4.49 -0.60 -3.00 -10.89 -3.18
Standard name
Human Ortholog
Description Cytoplasmic protein of unknown function
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Kel3

Kel3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kel3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available