Standard name | |
---|---|
Human Ortholog | |
Description | Mu1-like medium subunit of the AP-1 complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; the AP-1 complex is the clathrin-associated protein complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 8 | 19 | 8 | 3 | 7 | – | 5 | 8 | 1 | 8 | 2 | 40 | 21 | 21 | 4 | 2 | 7 | 17 | 9 | 29 | 29 | 48 | 52 |
Bud | 7 | 4 | 13 | 8 | 7 | 8 | – | 25 | 17 | 20 | 28 | 16 | 12 | 5 | 9 | 0 | 0 | 0 | 2 | 0 | 0 | 8 | 7 | 11 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | – | 6 | 3 | 8 | 7 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 1 | 1 | 0 | 2 | – | 1 | 2 | 4 | 3 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 6 | 20 | 13 | 14 | 17 | – | 19 | 20 | 6 | 11 | 7 | 17 | 21 | 32 | 4 | 0 | 9 | 1 | 1 | 0 | 1 | 1 | 3 |
Endoplasmic Reticulum | 7 | 19 | 15 | 8 | 6 | 18 | – | 7 | 4 | 4 | 2 | 6 | 29 | 14 | 10 | 2 | 1 | 1 | 2 | 2 | 3 | 11 | 16 | 13 |
Endosome | 28 | 1 | 33 | 17 | 37 | 18 | – | 79 | 21 | 11 | 20 | 14 | 28 | 13 | 28 | 2 | 2 | 5 | 58 | 31 | 66 | 34 | 50 | 47 |
Golgi | 70 | 19 | 182 | 50 | 77 | 33 | – | 20 | 20 | 16 | 1 | 17 | 109 | 65 | 58 | 5 | 11 | 23 | 150 | 49 | 215 | 60 | 162 | 149 |
Mitochondria | 91 | 39 | 156 | 103 | 86 | 235 | – | 289 | 320 | 263 | 279 | 272 | 24 | 16 | 5 | 1 | 2 | 9 | 10 | 9 | 15 | 25 | 45 | 25 |
Nucleus | 0 | 0 | 1 | 1 | 0 | 2 | – | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 3 | – | 2 | 1 | 5 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 3 |
Nucleolus | 14 | 2 | 4 | 5 | 4 | 6 | – | 25 | 20 | 15 | 14 | 19 | 3 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 4 | 2 | 4 | 2 | – | 37 | 3 | 2 | 7 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 5 | 5 | 8 | 8 |
SpindlePole | 2 | 0 | 0 | 0 | 4 | 3 | – | 18 | 7 | 5 | 9 | 7 | 4 | 4 | 4 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 1 |
Vac/Vac Membrane | 50 | 9 | 28 | 8 | 16 | 15 | – | 23 | 4 | 13 | 11 | 13 | 47 | 27 | 101 | 3 | 2 | 7 | 16 | 24 | 8 | 10 | 15 | 15 |
Unique Cell Count | 211 | 78 | 314 | 154 | 186 | 305 | 438 | 375 | 296 | 320 | 316 | 240 | 137 | 231 | 17 | 18 | 44 | 268 | 132 | 350 | 191 | 363 | 340 | |
Labelled Cell Count | 285 | 107 | 476 | 224 | 258 | 370 | 556 | 452 | 374 | 403 | 391 | 316 | 190 | 272 | 22 | 21 | 63 | 268 | 132 | 350 | 191 | 363 | 340 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.5 | 9.7 | 10.0 | 9.4 | 9.3 | 7.6 | – | 7.7 | 7.2 | 7.0 | 6.6 | 7.1 | 9.9 | 10.1 | 10.3 | 8.7 | 9.1 | 11.0 | 9.8 | 10.5 | 10.7 |
Std Deviation (1e-4) | 1.6 | 1.5 | 1.4 | 1.4 | 1.6 | 1.4 | – | 1.6 | 1.5 | 1.6 | 1.6 | 1.5 | 1.2 | 1.4 | 2.0 | 1.7 | 1.4 | 2.5 | 1.7 | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.11 | -0.4 | – | -0.39 | -0.48 | -0.52 | -0.61 | -0.49 | -0.02 | 0.01 | 0.04 | -0.2 | -0.14 | 0.13 | -0.03 | 0.07 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.4 | -2.3 | -2.3 | – | -3.8 | -2.6 | -4.0 | -2.2 | -3.8 | 4.0 | 3.2 | 1.3 | 0 | 0 | 0 |
Bud | 0.5 | -0.2 | -1.0 | – | 1.0 | 0.3 | 1.4 | 2.4 | 0.6 | 0.5 | -0.2 | -0.1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.8 | 0.5 | -0.4 | – | -1.2 | -0.6 | -2.7 | -1.7 | -2.6 | 0.3 | 3.0 | 2.9 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.2 | -0.8 | 0.6 | – | -2.6 | -3.0 | -2.4 | -3.2 | -2.0 | 3.2 | 2.2 | -0.2 | 0 | 0 | 0 |
Endosome | 0.2 | 2.9 | -2.1 | – | 2.9 | -2.4 | -3.2 | -1.9 | -2.9 | 0.4 | -0.3 | 0.6 | 0 | 0 | 0 |
Golgi | -5.2 | -3.6 | -12.3 | – | -16.3 | -15.1 | -13.9 | -16.0 | -14.2 | -2.9 | -2.1 | -7.6 | -2.3 | 0.3 | -0.7 |
Mitochondria | 3.5 | -0.7 | 7.1 | – | 4.5 | 10.1 | 10.4 | 10.2 | 9.8 | -9.9 | -7.6 | -12.0 | -3.5 | -3.2 | -3.6 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 3.1 | 2.9 | 2.7 | 2.4 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 4.3 | 0 | 0 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.4 | -0.1 | -2.0 | – | -2.0 | -4.9 | -2.2 | -2.9 | -2.4 | 3.6 | 3.2 | 9.4 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.4694 | 7.6038 | 7.3288 | 6.8251 | 6.8832 | 7.4848 | 8.5565 | 9.558 | 8.8958 | 8.965 | 8.221 | 9.1184 | 10.1868 | 10.3889 | 9.9837 | 10.2498 | 9.2484 | 10.1868 |
Actin | 0.0449 | 0.0128 | 0.0099 | 0.0013 | 0.0094 | 0.004 | 0.0174 | 0.0108 | 0.0222 | 0.0054 | 0.0038 | 0.02 | 0.0295 | 0.0137 | 0.0238 | 0.0054 | 0.0034 | 0.0065 |
Bud | 0.0004 | 0.0004 | 0.0028 | 0.0026 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0.0025 | 0.0004 | 0.0004 | 0.0003 | 0.0008 | 0.0006 | 0.0011 | 0.0009 | 0.0002 | 0.0004 |
Bud Neck | 0.0031 | 0.0005 | 0.001 | 0.0004 | 0.0006 | 0.0006 | 0.0005 | 0.0005 | 0.0007 | 0.0004 | 0.0003 | 0.0012 | 0.0006 | 0.0008 | 0.0011 | 0.0004 | 0.0003 | 0.0003 |
Bud Periphery | 0.0004 | 0.0003 | 0.0024 | 0.0034 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0024 | 0.0003 | 0.0004 | 0.0003 | 0.001 | 0.0005 | 0.001 | 0.001 | 0.0002 | 0.0008 |
Bud Site | 0.0036 | 0.0015 | 0.0032 | 0.0009 | 0.0003 | 0.0004 | 0.0008 | 0.0019 | 0.0049 | 0.0004 | 0.0004 | 0.0008 | 0.0022 | 0.0027 | 0.003 | 0.0005 | 0.0002 | 0.0003 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0122 | 0.0086 | 0.0094 | 0.0068 | 0.0024 | 0.0071 | 0.0027 | 0.0066 | 0.0037 | 0.0024 | 0.002 | 0.0021 | 0.0034 | 0.0214 | 0.0037 | 0.0025 | 0.0029 | 0.0016 |
Cytoplasmic Foci | 0.1081 | 0.1351 | 0.1011 | 0.0592 | 0.0606 | 0.0803 | 0.0978 | 0.1119 | 0.0864 | 0.0749 | 0.0794 | 0.0675 | 0.0801 | 0.1208 | 0.0842 | 0.0606 | 0.075 | 0.0612 |
Eisosomes | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0077 | 0.0012 | 0.0029 | 0.0016 | 0.0024 | 0.0017 | 0.0016 | 0.0007 | 0.0007 | 0.0004 | 0.0005 | 0.0009 | 0.0008 | 0.0005 | 0.0008 | 0.0006 | 0.0009 | 0.0008 |
Endosome | 0.3044 | 0.4027 | 0.3171 | 0.4654 | 0.3449 | 0.2877 | 0.2704 | 0.3958 | 0.2859 | 0.3356 | 0.319 | 0.273 | 0.3484 | 0.3392 | 0.3147 | 0.3931 | 0.3834 | 0.2448 |
Golgi | 0.4149 | 0.3883 | 0.4942 | 0.392 | 0.5058 | 0.5837 | 0.5348 | 0.4492 | 0.5304 | 0.5472 | 0.5588 | 0.5895 | 0.4904 | 0.4562 | 0.5207 | 0.5068 | 0.5061 | 0.6484 |
Lipid Particles | 0.0205 | 0.0086 | 0.0084 | 0.0039 | 0.0118 | 0.0043 | 0.0102 | 0.0026 | 0.0049 | 0.0018 | 0.0056 | 0.0047 | 0.005 | 0.0033 | 0.0042 | 0.0014 | 0.0027 | 0.0053 |
Mitochondria | 0.0182 | 0.0096 | 0.0129 | 0.015 | 0.0116 | 0.0162 | 0.0328 | 0.0098 | 0.0284 | 0.0181 | 0.0193 | 0.0238 | 0.0265 | 0.0254 | 0.017 | 0.0145 | 0.006 | 0.0131 |
None | 0.0116 | 0.0005 | 0.0006 | 0.0001 | 0.0017 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.011 | 0.0005 | 0.0062 | 0.0008 | 0.0211 | 0.0005 | 0.0026 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0011 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0008 |
Nucleolus | 0.0007 | 0.0003 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.003 | 0.0004 | 0.0048 | 0.0003 | 0.0034 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Peroxisomes | 0.0239 | 0.0134 | 0.0124 | 0.0021 | 0.0033 | 0.0056 | 0.0218 | 0.0046 | 0.0158 | 0.0092 | 0.0051 | 0.0062 | 0.0035 | 0.007 | 0.0146 | 0.002 | 0.0018 | 0.0103 |
Punctate Nuclear | 0.0043 | 0.0029 | 0.0018 | 0.0001 | 0.0081 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Vacuole | 0.0035 | 0.0079 | 0.0046 | 0.0306 | 0.0065 | 0.0038 | 0.0026 | 0.0023 | 0.0062 | 0.0012 | 0.0019 | 0.004 | 0.0035 | 0.0046 | 0.0059 | 0.0049 | 0.0082 | 0.002 |
Vacuole Periphery | 0.0031 | 0.0044 | 0.0035 | 0.0132 | 0.0049 | 0.003 | 0.0025 | 0.0024 | 0.0033 | 0.0021 | 0.0025 | 0.0037 | 0.0032 | 0.0025 | 0.0033 | 0.0048 | 0.0079 | 0.003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.09 | 14.5055 | 19.9898 | 25.1993 | 15.5284 | 16.9898 | 26.8886 | 22.3904 | 24.3594 | 26.0037 |
Translational Efficiency | 0.848 | 1.2531 | 0.8297 | 0.8311 | 0.9083 | 0.9979 | 0.8395 | 0.9214 | 0.8231 | 0.7501 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
92 | 976 | 510 | 829 | 2229 | 1553 | 2561 | 1820 | 2321 | 2529 | 3071 | 2649 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1243.92 | 1140.51 | 1299.37 | 1231.91 | 919.98 | 1044.37 | 1190.23 | 1246.05 | 932.82 | 1081.47 | 1208.35 | 1241.62 |
Standard Deviation | 162.37 | 172.57 | 165.71 | 171.04 | 120.81 | 144.18 | 162.20 | 154.62 | 138.04 | 162.63 | 167.78 | 160.08 |
Intensity Change Log 2 | — | -0.125215 | 0.062919 | -0.013997 | — | 0.182959 | 0.371566 | 0.437688 | — | 0.013920 | 0.202280 | 0.195519 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003705 | 0.004423 | 0.002606 | 0.002585 | 0.002109 | 0.001305 | 0.001408 | 0.002080 | 0.002173 | 0.002508 | 0.001607 | 0.002238 |
Bud Neck | 0.001720 | 0.000430 | 0.001528 | 0.001301 | 0.000521 | 0.000540 | 0.000202 | 0.000439 | 0.000568 | 0.000497 | 0.000422 | 0.000708 |
Bud Site | 0.046225 | 0.013006 | 0.012317 | 0.025625 | 0.013195 | 0.007929 | 0.006431 | 0.016818 | 0.014505 | 0.009888 | 0.007409 | 0.019574 |
Cell Periphery | 0.000270 | 0.000216 | 0.000199 | 0.000672 | 0.000159 | 0.000153 | 0.000077 | 0.000226 | 0.000164 | 0.000178 | 0.000097 | 0.000366 |
Cytoplasm | 0.012394 | 0.003598 | 0.002726 | 0.012632 | 0.005095 | 0.004541 | 0.004232 | 0.010916 | 0.005384 | 0.004177 | 0.003982 | 0.011453 |
Cytoplasmic Foci | 0.121022 | 0.171529 | 0.128089 | 0.100545 | 0.136463 | 0.169569 | 0.088513 | 0.080912 | 0.135851 | 0.170325 | 0.095086 | 0.087056 |
Eisosomes | 0.000030 | 0.000045 | 0.000025 | 0.000027 | 0.000065 | 0.000035 | 0.000017 | 0.000020 | 0.000064 | 0.000039 | 0.000018 | 0.000022 |
Endoplasmic Reticulum | 0.001936 | 0.000826 | 0.003492 | 0.001355 | 0.001229 | 0.000948 | 0.000290 | 0.000700 | 0.001257 | 0.000901 | 0.000822 | 0.000905 |
Endosome | 0.211339* | 0.146220 | 0.146075 | 0.148324 | 0.164745 | 0.207850* | 0.129544 | 0.125571 | 0.166592 | 0.184065 | 0.132290 | 0.132692 |
Golgi | 0.180598* | 0.602983* | 0.475080* | 0.340452* | 0.545272* | 0.484395* | 0.618179* | 0.474266* | 0.530817* | 0.530161* | 0.594415* | 0.432390* |
Lipid Particles | 0.000996 | 0.000932 | 0.002923 | 0.001308 | 0.001286 | 0.003462 | 0.000999 | 0.000562 | 0.001274 | 0.002485 | 0.001318 | 0.000795 |
Mitochondria | 0.011340 | 0.017387 | 0.001828 | 0.006293 | 0.079818 | 0.039789 | 0.012412 | 0.008591 | 0.077104 | 0.031144 | 0.010654 | 0.007872 |
Mitotic Spindle | 0.013876 | 0.005108 | 0.019698 | 0.014386 | 0.003237 | 0.008764 | 0.007199 | 0.006233 | 0.003659 | 0.007353 | 0.009275 | 0.008784 |
None | 0.000021 | 0.000266 | 0.000040 | 0.000235 | 0.000070 | 0.000194 | 0.000035 | 0.000054 | 0.000068 | 0.000222 | 0.000036 | 0.000111 |
Nuclear Periphery | 0.000188 | 0.000044 | 0.000707 | 0.000606 | 0.000046 | 0.000137 | 0.000105 | 0.000115 | 0.000051 | 0.000101 | 0.000205 | 0.000269 |
Nuclear Periphery Foci | 0.000872 | 0.000229 | 0.004759 | 0.002325 | 0.000174 | 0.000517 | 0.000603 | 0.000631 | 0.000201 | 0.000406 | 0.001293 | 0.001161 |
Nucleolus | 0.000033 | 0.000107 | 0.000319 | 0.000120 | 0.000025 | 0.000087 | 0.000011 | 0.000039 | 0.000026 | 0.000095 | 0.000062 | 0.000064 |
Nucleus | 0.000172 | 0.000058 | 0.000303 | 0.000096 | 0.000041 | 0.000437 | 0.000020 | 0.000045 | 0.000046 | 0.000291 | 0.000067 | 0.000061 |
Peroxisomes | 0.003465 | 0.002081 | 0.001872 | 0.001967 | 0.002778 | 0.003497 | 0.000816 | 0.001128 | 0.002805 | 0.002951 | 0.000992 | 0.001391 |
Vacuole | 0.291707* | 0.013532 | 0.121761 | 0.226855* | 0.016309 | 0.029414 | 0.068718 | 0.148904 | 0.027225 | 0.023285 | 0.077527 | 0.173299 |
Vacuole Periphery | 0.098094 | 0.016979 | 0.073654 | 0.112292* | 0.027363 | 0.036438 | 0.060189 | 0.121748* | 0.030167 | 0.028928 | 0.062425 | 0.118789* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.28 | 0.42 | 0.44 | 1.39 | 0.02 | 1.50 | 1.10 | -0.07 | -1.28 | -0.99 | -0.51 | 0.94 | -0.20 | 0.32 | -1.14 |
Bud Neck | 3.74 | 0.53 | 0.98 | -4.36 | 0.59 | -0.27 | 4.89 | 0.88 | 1.08 | -4.73 | 0.89 | 1.88 | -1.82 | -2.72 | -3.67 |
Bud Site | 2.29 | 2.29 | 1.35 | -3.93 | -3.68 | 3.70 | 5.12 | -2.24 | -5.96 | -7.44 | 2.97 | 4.93 | -3.12 | -6.53 | -8.89 |
Cell Periphery | 0.57 | 0.85 | -1.27 | -1.45 | -1.52 | 0.12 | 5.68 | -2.68 | -1.69 | -6.36 | -0.46 | 4.52 | -2.06 | -1.84 | -2.75 |
Cytoplasm | 2.51 | 2.71 | -0.14 | -5.48 | -5.79 | 0.69 | 1.30 | -5.27 | -5.30 | -5.88 | 2.04 | 2.36 | -6.41 | -7.61 | -7.83 |
Cytoplasmic Foci | -3.96 | -0.54 | 1.56 | 12.29 | 3.81 | -8.24 | 17.13 | 18.40 | 22.27 | 2.78 | -10.24 | 14.62 | 16.88 | 25.67 | 3.03 |
Eisosomes | -1.44 | 0.44 | 0.30 | 3.39 | -0.26 | 8.01 | 13.29 | 12.65 | 6.25 | -1.51 | 6.96 | 13.10 | 11.68 | 6.96 | -1.80 |
Endoplasmic Reticulum | 1.33 | -1.10 | 0.66 | -1.29 | 1.80 | 0.62 | 2.47 | 1.35 | 0.94 | -4.47 | 0.89 | 1.06 | 0.90 | -0.02 | -0.36 |
Endosome | 3.53 | 3.46 | 3.42 | -0.25 | -0.24 | -8.07 | 8.89 | 9.26 | 15.81 | 1.05 | -3.96 | 8.99 | 8.63 | 12.82 | -0.07 |
Golgi | -17.23 | -10.89 | -6.18 | 20.77 | 8.00 | 7.25 | -9.67 | 8.10 | 1.15 | 16.18* | 0.10 | -8.53 | 12.44 | 12.60 | 20.76* |
Lipid Particles | 0.34 | -2.42 | -0.48 | -1.29 | 2.38 | -2.80 | 0.66 | 2.19 | 3.91 | 1.28 | -2.29 | -0.12 | 1.47 | 3.56 | 1.68 |
Mitochondria | -0.64 | 0.98 | 0.52 | 4.91 | -3.55 | 9.99 | 21.04 | 22.32 | 11.53 | 3.12 | 13.24 | 21.55 | 22.55 | 12.33 | 2.75 |
Mitotic Spindle | 1.01 | -0.70 | -0.25 | -4.01 | 0.95 | -4.40 | -4.36 | -3.23 | 1.68 | 0.84 | -3.80 | -5.43 | -5.02 | -1.57 | 0.08 |
None | -1.06 | -2.41 | -1.15 | 0.12 | -1.05 | -1.23 | 7.41 | 2.71 | 1.37 | -4.82 | -1.43 | 7.09 | -0.74 | 0.93 | -1.28 |
Nuclear Periphery | 2.39 | -2.06 | -1.60 | -2.19 | 0.20 | -3.68 | -4.08 | -4.15 | 0.76 | -0.48 | -2.83 | -3.64 | -2.66 | -2.05 | -0.76 |
Nuclear Periphery Foci | 2.47 | -2.71 | -2.24 | -3.33 | 1.34 | -2.62 | -3.17 | -3.01 | -0.56 | -0.13 | -2.20 | -4.22 | -4.21 | -3.27 | 0.09 |
Nucleolus | -0.73 | -1.00 | -1.40 | -0.40 | 0.55 | -2.75 | 2.35 | -1.18 | 1.70 | -2.21 | -1.74 | -0.77 | -1.80 | 0.33 | -0.30 |
Nucleus | 1.42 | -0.34 | 1.06 | -1.20 | 0.87 | -2.35 | 3.32 | -0.84 | 2.31 | -5.21 | -2.32 | -0.52 | -1.89 | 2.16 | 0.10 |
Peroxisomes | 1.71 | 1.78 | 1.79 | 0.18 | -0.22 | -1.28 | 4.79 | 4.09 | 4.65 | -1.07 | -0.35 | 4.66 | 3.73 | 4.41 | -1.59 |
Vacuole | 9.66 | 5.74 | 2.23 | -24.73 | -9.09 | -7.06 | -20.43 | -29.64 | -25.47 | -15.91 | 1.93 | -16.67 | -32.66 | -35.37 | -20.06 |
Vacuole Periphery | 5.90 | 1.60 | -1.00 | -17.48 | -4.80 | -3.90 | -13.50 | -24.13* | -20.23 | -14.38 | 0.64 | -13.40 | -26.82 | -26.96 | -15.41 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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