ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.97 0.98 0.98 0.94 0.88 0.73 0.72 0.72 0.67 0.55 0.6 0.98 0.98 0.98 0.88 0.87 0.82 0.81 0.88 0.81 0.88 0.86 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0.06 0.08 0.31 0.45 0.41 0.5 0.56 0.64 0.62 0 0 0 0 0 0 0.07 0 0.11 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0.05 0.17 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 5 3 2 3 4 11
Bud 0 2 1 0 1 2 3 6 8 5 8 3 1 0 0 0 0 1 4 3 0 1 8 16
Bud Neck 0 2 0 1 1 0 2 1 2 0 0 2 0 0 0 0 0 0 1 1 0 2 1 5
Bud Site 0 0 0 0 0 0 0 2 2 2 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 9 1 0 1 0 4 1 2 1 2 1 0 1 8 2 11 4 1 2 2 1 1 6
Cytoplasm 369 477 224 157 235 168 177 213 252 252 162 172 654 720 875 455 450 282 307 467 184 351 385 316
Endoplasmic Reticulum 1 2 1 0 0 0 0 0 0 0 4 2 1 1 0 17 27 10 1 1 2 2 2 3
Endosome 0 3 1 0 2 1 0 0 0 0 0 0 1 4 2 24 19 21 2 4 1 2 5 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 4 1 0 1 0 0 1 1
Mitochondria 34 9 4 10 21 59 110 122 175 208 187 177 1 2 0 6 9 3 25 20 24 12 12 23
Nucleus 2 1 0 1 3 0 1 2 2 7 2 4 1 2 5 7 6 5 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 1 2 2 0 0 0 1 0 1 0 0 0 1 0 2
Nucleolus 0 0 0 0 0 2 0 1 0 0 1 0 1 0 0 4 1 4 0 0 0 0 0 0
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 1 1 0 0 0 0 1
SpindlePole 0 1 0 0 1 0 0 1 2 0 0 0 1 0 0 23 15 22 6 4 5 5 15 20
Vac/Vac Membrane 0 3 0 0 0 4 4 10 8 17 3 15 9 18 26 27 90 28 9 8 0 3 2 11
Unique Cell Count 384 491 228 161 249 191 243 297 350 374 294 287 664 738 895 516 518 346 381 532 229 397 451 441
Labelled Cell Count 408 510 232 169 265 236 301 360 453 493 374 378 670 749 917 568 632 383 381 532 229 397 451 441


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.0 3.6 3.4 3.4 3.8 3.1 3.6 3.4 3.2 3.4 3.3 5.1 5.3 5.6 6.1 7.4 6.8 3.8 4.0 4.7
Std Deviation (1e-4) 0.8 0.7 0.8 1.0 1.0 2.0 1.2 1.4 1.0 1.0 1.3 1.0 1.4 1.5 1.3 1.2 1.6 1.5 1.1 1.4 1.3
Intensity Change (Log2) – – – -0.07 -0.07 0.07 -0.19 -0.01 -0.09 -0.16 -0.07 -0.11 0.52 0.58 0.63 0.78 1.05 0.92 0.07 0.16 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.5 6.7 4.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8967 3.1437 3.224 3.1897 3.1028 3.2981 2.5557 3.2124 3.1966 3.0412 3.1907 3.2588 3.0459 3.3804 3.7164 3.628 3.1641 3.3541
Actin 0.0117 0.0008 0.0123 0.0055 0.0149 0.0074 0.0034 0.0018 0.0061 0.0004 0.0023 0.0023 0.0013 0.0037 0.0007 0.0114 0.0005 0.0012
Bud 0.0051 0.0009 0.0084 0.0251 0.0036 0.0023 0.0027 0.004 0.0011 0.0036 0.0171 0.0091 0.0002 0.0029 0.001 0.0079 0.0009 0.0005
Bud Neck 0.0019 0.0005 0.0007 0.0005 0.003 0.0009 0.0005 0.0004 0.0003 0.0001 0.0049 0.0033 0.0005 0.0003 0.0003 0.0006 0.0002 0.0005
Bud Periphery 0.0107 0.0034 0.009 0.0218 0.0027 0.0011 0.002 0.0009 0.0007 0.0063 0.0088 0.0045 0.0006 0.0011 0.0016 0.004 0.0003 0.0005
Bud Site 0.0071 0.0176 0.0138 0.0009 0.0029 0.0005 0.0029 0.0066 0.0036 0.0003 0.0278 0.0007 0.0008 0.0154 0.009 0.0017 0.0003 0.0001
Cell Periphery 0.0017 0.0004 0.0004 0.0004 0.0004 0.0001 0.0002 0.0002 0.0002 0.0001 0.0003 0.0002 0.0001 0.0003 0.0001 0.0002 0 0
Cytoplasm 0.0797 0.1033 0.1173 0.0835 0.09 0.1529 0.1962 0.2597 0.212 0.1496 0.1214 0.2604 0.1334 0.1875 0.0827 0.1349 0.1335 0.1857
Cytoplasmic Foci 0.0209 0.0056 0.0165 0.0058 0.0079 0.0201 0.0079 0.0068 0.0082 0.0039 0.0086 0.0075 0.0061 0.0056 0.0103 0.0137 0.004 0.0077
Eisosomes 0.0017 0.0002 0.0003 0.0003 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0058 0.0013 0.0015 0.0014 0.0019 0.0014 0.0022 0.0009 0.0014 0.0006 0.0004 0.0012 0.0017 0.0012 0.0015 0.0013 0.0006 0.0009
Endosome 0.0116 0.0009 0.0035 0.0018 0.0094 0.0028 0.0067 0.0012 0.0017 0.0008 0.0004 0.0011 0.002 0.0008 0.0025 0.0052 0.0007 0.0011
Golgi 0.0049 0.0001 0.0018 0.0003 0.0021 0.0007 0.0008 0.0002 0.0002 0.0001 0.0002 0.0001 0.0003 0.0001 0.0002 0.0024 0 0.0001
Lipid Particles 0.0122 0.0003 0.0036 0.0003 0.0072 0.0018 0.0013 0.0005 0.0002 0.0001 0.0002 0.0003 0.0004 0.0002 0.0005 0.0069 0.0001 0.0002
Mitochondria 0.011 0.0003 0.0015 0.0021 0.0033 0.0011 0.0009 0.0003 0.0004 0.0004 0.0009 0.0004 0.0004 0.0002 0.0006 0.0013 0.0002 0.0005
None 0.7778 0.8554 0.7945 0.842 0.7947 0.7787 0.7514 0.7064 0.7461 0.8231 0.7826 0.6958 0.8421 0.768 0.8808 0.7698 0.8356 0.7957
Nuclear Periphery 0.0035 0.0004 0.0011 0.0009 0.0093 0.0035 0.0043 0.0008 0.0015 0.0007 0.0005 0.001 0.0012 0.0005 0.0014 0.0031 0.0012 0.0008
Nucleolus 0.0025 0.0007 0.0006 0.0005 0.0039 0.0006 0.0014 0.0005 0.0006 0.0002 0.0013 0.0008 0.0004 0.0003 0.0005 0.0009 0.0006 0.0002
Nucleus 0.008 0.0036 0.0029 0.0036 0.0255 0.0057 0.0048 0.0041 0.0066 0.0067 0.0054 0.0046 0.0028 0.0038 0.0017 0.0037 0.012 0.0013
Peroxisomes 0.0095 0.0002 0.0038 0.0003 0.0032 0.0024 0.0005 0.0007 0.0004 0.0002 0.0007 0.0003 0.0003 0.0001 0.0009 0.0024 0.0002 0.0002
Punctate Nuclear 0.0057 0.0032 0.0051 0.0014 0.0086 0.015 0.0081 0.0031 0.0076 0.0022 0.0152 0.0052 0.0045 0.0068 0.0029 0.0273 0.0083 0.0022
Vacuole 0.0054 0.0006 0.0013 0.0014 0.0034 0.0007 0.0013 0.0007 0.0008 0.0005 0.0006 0.0009 0.0007 0.0007 0.0007 0.0007 0.0006 0.0003
Vacuole Periphery 0.0016 0.0001 0.0002 0.0002 0.0016 0.0003 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.0191 5.6472 12.1058 12.8643 7.2805 16.8365 11.1025 16.2715 18.6636 9.889
Translational Efficiency 0.6539 0.7617 0.4914 0.7112 0.78 0.4384 0.632 0.6357 0.638 0.5583

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
215 1930 192 81 2314 1931 1339 1269 2529 3861 1531 1350

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 656.39 611.58 849.63 868.43 655.51 675.02 733.11 1110.53 655.58 643.31 747.72 1096.00
Standard Deviation 61.71 82.33 100.95 162.94 72.02 90.96 98.88 819.63 71.21 92.37 106.39 797.74
Intensity Change Log 2 — -0.102012 0.372281 0.403856 — 0.042312 0.161412 0.760559 — -0.028094 0.270767 0.593085

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000020 0.000360 0.001188 0.006252 0.000162 0.000407 0.000078 0.000084 0.000150 0.000383 0.000218 0.000454
Bud Neck 0.008462 0.044623 0.001883 0.064119 0.017016 0.054951 0.005593 0.007830 0.016289 0.049788 0.005128 0.011208
Bud Site 0.000587 0.015289 0.001887 0.057501 0.002484 0.022199 0.007749 0.006698 0.002322 0.018745 0.007014 0.009746
Cell Periphery 0.000197 0.000119 0.000211 0.000359 0.000147 0.000146 0.000446 0.000132 0.000151 0.000132 0.000417 0.000146
Cytoplasm 0.501111* 0.416312* 0.189028* 0.144951* 0.451128* 0.409329* 0.458669* 0.619916* 0.455377* 0.412819* 0.424854* 0.591418*
Cytoplasmic Foci 0.166107 0.227292* 0.015142 0.118220 0.178390 0.237650* 0.019893 0.004474 0.177346 0.232472* 0.019297 0.011298
Eisosomes 0.000205 0.000128 0.000091 0.000103 0.000182 0.000144 0.000085 0.000027 0.000184 0.000136 0.000086 0.000031
Endoplasmic Reticulum 0.000567 0.000757 0.006488 0.002506 0.000978 0.000776 0.000527 0.000297 0.000943 0.000767 0.001274 0.000429
Endosome 0.004937 0.024887 0.004750 0.077782 0.008308 0.021731 0.001917 0.000571 0.008022 0.023309 0.002273 0.005204
Golgi 0.000575 0.004767 0.000477 0.020673 0.002209 0.005205 0.000290 0.000120 0.002070 0.004986 0.000314 0.001353
Lipid Particles 0.011553 0.010378 0.004414 0.007173 0.014223 0.007511 0.000947 0.000139 0.013996 0.008944 0.001382 0.000561
Mitochondria 0.002498 0.014269 0.000419 0.015712 0.006388 0.015237 0.000717 0.000951 0.006057 0.014753 0.000679 0.001837
Mitotic Spindle 0.000012 0.006089 0.006715 0.113232* 0.002735 0.007649 0.007064 0.027365 0.002503 0.006869 0.007020 0.032517
None 0.002631 0.006198 0.005319 0.002590 0.003824 0.003541 0.014874 0.017124 0.003723 0.004869 0.013676 0.016252
Nuclear Periphery 0.000698 0.000737 0.002253 0.001649 0.000982 0.000538 0.000717 0.000626 0.000958 0.000637 0.000910 0.000688
Nuclear Periphery Foci 0.000188 0.000348 0.002585 0.010523 0.000221 0.000446 0.000299 0.000245 0.000218 0.000397 0.000585 0.000862
Nucleolus 0.001260 0.003815 0.001980 0.002464 0.001336 0.002531 0.001699 0.001181 0.001330 0.003173 0.001735 0.001258
Nucleus 0.262006* 0.129755 0.682042* 0.175872* 0.268224* 0.117566 0.449333* 0.296354* 0.267696* 0.123659 0.478516* 0.289125*
Peroxisomes 0.001830 0.014875 0.005612 0.055673 0.005103 0.018335 0.000443 0.000391 0.004825 0.016606 0.001091 0.003708
Vacuole 0.034239 0.076316 0.067269 0.107207 0.034998 0.071697 0.028285 0.015319 0.034933 0.074006 0.033174 0.020833
Vacuole Periphery 0.000318 0.002686 0.000247 0.015439 0.000961 0.002412 0.000375 0.000156 0.000906 0.002549 0.000359 0.001073

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.01 -2.43 -2.09 -1.92 -1.50 -2.51 0.91 0.33 4.04 -1.88 -2.94 -0.87 -1.66 -0.04 -1.14
Bud Neck -10.06 1.80 -2.54 1.04 -3.17 -12.75 7.65 0.79 12.69 -5.80 -15.96 8.03 -0.18 14.16 -6.70
Bud Site -11.90 -4.98 -5.57 -3.55 -5.25 -11.21 -3.11 -6.06 5.78 -1.64 -14.75 -3.26 -7.60 4.13 -3.14
Cell Periphery 4.06 -1.50 -2.07 -4.09 -1.09 0.16 -5.95 3.39 3.08 6.90 2.49 -6.12 3.07 1.81 6.97
Cytoplasm 5.25 16.19 12.30 10.51 0.04 5.07 1.46 -3.70 -7.53 -4.40 6.46 5.82 -1.29 -6.09 -5.89
Cytoplasmic Foci -5.81 15.54 6.25 11.50 -3.65 -9.16 38.27 42.10 44.89 7.54 -10.82 40.83 42.64 60.02 4.75
Eisosomes 3.20 3.23 1.63 -1.68 -1.83 2.37 6.40 9.23 12.61 6.06 3.30 6.72 9.53 12.11 6.04
Endoplasmic Reticulum -1.12 -8.37 -6.11 -5.99 1.04 1.14 0.73 3.54 0.88 3.18 1.51 -5.62 -0.97 -2.38 4.83
Endosome -13.80 -0.67 -3.27 -1.09 -3.19 -11.37 9.97 12.98 19.74 2.98 -15.83 9.55 6.38 19.96 -0.85
Golgi -8.55 0.53 -1.61 -0.58 -1.66 -5.29 7.36 7.96 9.72 -0.04 -7.48 7.38 4.24 10.81 -1.51
Lipid Particles 0.45 2.32 1.33 1.61 -1.43 5.92 13.93 14.64 11.58 9.07 4.90 13.82 14.67 14.95 4.74
Mitochondria -5.34 1.07 -1.04 3.29 -2.19 -5.63 6.30 5.81 10.94 -1.62 -7.20 6.41 5.45 15.20 -2.55
Mitotic Spindle -5.45 -1.56 -3.64 -3.38 -3.13 -3.10 -2.66 -10.22 -9.10 -8.38 -3.64 -2.97 -10.85 -9.96 -8.93
None -3.36 -2.03 -1.32 1.15 0.18 0.38 -5.16 -14.99 -15.63 -2.78 -1.57 -5.25 -15.13 -14.18 -3.26
Nuclear Periphery -0.19 -9.41 -6.21 -6.01 -1.66 3.07 -0.17 -4.36 -7.33 -5.19 2.31 -2.31 -5.36 -7.52 -4.56
Nuclear Periphery Foci -4.72 -3.47 -2.83 -2.81 -2.43 -2.00 -3.52 -5.26 -2.19 -2.72 -3.09 -4.46 -3.53 -3.22 -2.38
Nucleolus -3.98 -1.31 -2.36 -1.14 -1.62 -4.09 -0.71 -6.23 -5.11 -5.79 -6.74 -1.13 -6.45 -4.76 -5.89
Nucleus 9.53 -16.62 0.28 -3.95 11.13 25.89 -20.65 -10.49 -29.32 8.92 28.16 -24.65 -10.14 -29.61 12.30
Peroxisomes -10.73 -0.77 -2.68 -1.15 -2.33 -10.52 10.57 9.97 14.96 -0.65 -13.67 7.52 4.16 15.48 -1.74
Vacuole -9.48 -5.39 -6.27 -4.74 -3.69 -13.36 -2.87 0.52 11.38 2.77 -18.58 -5.16 -2.89 8.93 1.52
Vacuole Periphery -11.38 0.87 -1.24 -0.63 -1.25 -4.60 4.21 6.92 7.87 2.80 -7.80 4.32 1.90 7.21 -0.31
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

YPL247C

YPL247C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypl247c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available