Standard name
Description GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; human homolog RP2 complements yeast null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.96 0.95 0.96 0.86 0.71 0.69 0.49 0.62 0.48 0.42 1.0 0.98 1.0 0.97 0.97 0.97 0.9 0.92 0.82 0.89 0.86 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.08 0.05 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0.12 0.48 0.39 0.5 0.52 0 0 0 0 0 0 0 0 0.07 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0.12 0.16 0.1 0.06 0.1 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 3 3 2 1 1 2 5 3 0 1 0 0 1 0 0 0 3 3 3 3
Bud 0 3 0 0 2 1 1 4 4 8 7 1 1 0 0 0 0 0 0 1 1 3 4
Bud Neck 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 3 1 2 2
Bud Site 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Cell Periphery 2 2 12 7 4 11 5 2 7 4 5 2 6 5 5 2 3 0 0 0 0 0 0
Cytoplasm 137 221 246 193 204 240 209 114 188 126 149 300 539 617 193 189 190 131 229 221 242 240 284
Endoplasmic Reticulum 0 1 2 3 5 8 9 2 1 0 6 1 4 1 5 7 9 0 0 4 1 1 3
Endosome 0 0 1 2 10 27 14 10 10 12 23 0 0 0 2 0 0 5 3 4 2 4 6
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 0 1 0
Mitochondria 1 10 0 7 2 39 35 113 119 132 185 0 1 0 6 6 3 1 7 18 6 8 13
Nucleus 1 0 0 2 0 2 2 4 3 4 5 1 1 1 1 0 1 0 1 0 2 3 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 3 1 4 1 4 2 5 0 0 0 0 0 1 1 0 3 1 1 3
Vac/Vac Membrane 4 0 7 0 20 40 47 24 19 25 40 0 2 9 1 1 3 1 2 1 5 2 4
Unique Cell Count 141 230 260 202 237 336 303 233 303 262 358 301 548 619 198 194 195 147 251 272 273 280 341
Labelled Cell Count 146 238 272 217 254 372 328 276 358 318 432 305 555 633 213 207 211 147 251 272 273 280 341


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 5.1 7.1 7.0 7.6 6.2 6.1 5.2 5.3 5.3 5.2 6.6 6.4 6.3 5.4 6.4 6.4 7.3 7.3 7.0
Std Deviation (1e-4) 0.8 0.7 1.0 1.8 1.8 1.5 1.7 1.3 1.0 1.6 0.9 0.9 1.0 1.0 1.1 1.1 1.3 1.8 1.4 1.2
Intensity Change (Log2) -0.04 0.09 -0.22 -0.23 -0.47 -0.44 -0.43 -0.46 -0.11 -0.15 -0.19 -0.39 -0.16 -0.17 0.03 0.03 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.6 -1.8 -0.8 -2.0 -2.5 -1.5 -2.0 -2.4 -3.0 -3.2 -3.7 -1.2 -2.2 -1.8
Cytoplasm 0.5 -3.2 -7.2 -7.7 -11.4 -9.2 -11.7 -13.5 3.7 3.0 5.1 1.5 1.5 1.5
Endoplasmic Reticulum 0 0 0 1.9 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.9 4.4 3.1 2.9 2.5 3.1 3.8 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.7 5.7 12.8 11.4 13.2 13.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.8 4.1 5.1 3.5 2.0 3.3 3.9 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cin2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available