Standard name
Human Ortholog
Description Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.09 0 – 0.09 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.08 0.05 0.06 0.06 0.09 – 0.07 0 0.05 0.05 0.06 0.12 0.11 0.08 0.07 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.17 0.12 0.12 0.12 0.1 0 0.08 – 0.05 0 0 0 0.21 0.23 0.21 0.23 0.21 0.18 0 0 0 0 0.07 0.11
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0 0 0 0.07 0 – 0.05 0 0.08 0.06 0 0 0 0.08 0.09 0.07 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0.05 0 0.05 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.8 0.73 0.42 0.48 0.45 0.41 0.35 – 0.45 0.45 0.49 0.36 0.72 0.62 0.57 0.55 0.51 0.59 0 0.05 0 0 0.05 0.05
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.66 0.65 0.82 0.83 0.85 0.76 0.78 – 0.75 0.78 0.67 0.81 0.68 0.6 0.61 0.58 0.59 0.58 0.89 0.85 0.86 0.84 0.74 0.68
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 1 2 2 6 6 – 8 7 1 6 0 0 0 3 0 0 1 1 0 3 5 9
Bud Neck 1 9 0 4 3 8 5 – 8 10 6 10 0 2 0 0 0 1 0 1 1 1 3 3
Bud Site 2 8 2 1 3 19 5 – 18 11 8 17 1 1 3 3 5 4 – – – – – –
Cell Periphery 0 1 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 3 12 8 14 13 27 – 14 12 10 13 9 35 32 10 12 14 1 1 4 1 2 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 2 1 2 3 1 0 0 0 0 0 0
Endosome 16 38 19 18 22 3 25 – 10 4 3 8 30 64 60 30 34 20 4 10 5 4 21 31
Golgi 0 0 0 0 1 0 0 – 0 0 1 0 0 0 0 2 1 0 0 2 0 1 4 3
Mitochondria 3 27 1 1 1 15 4 – 10 11 15 17 4 3 0 10 15 8 0 1 0 1 3 1
Nucleus 0 6 4 6 6 9 5 – 10 8 10 8 0 8 9 8 7 6 0 5 1 1 6 5
Nuclear Periphery 0 2 0 0 0 1 1 – 0 0 1 0 0 0 1 0 1 1 0 0 0 0 1 2
Nucleolus 76 225 66 75 101 83 110 – 94 121 93 96 105 175 159 72 83 66 0 18 6 6 15 15
Peroxisomes 0 4 1 4 1 0 11 – 2 4 4 7 3 5 12 0 1 0 0 1 0 1 3 1
SpindlePole 63 202 128 128 191 156 245 – 156 210 128 214 100 169 171 76 96 65 93 282 131 154 216 188
Vac/Vac Membrane 3 5 3 2 0 1 4 – 0 0 1 0 1 3 2 7 11 8 1 2 0 1 2 2
Unique Cell Count 95 309 156 155 225 204 314 209 270 191 264 146 280 281 132 162 112 105 334 154 184 293 277
Labelled Cell Count 167 532 238 249 345 314 448 330 398 281 397 253 467 450 223 269 194 105 334 154 184 293 277


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 7.1 7.8 7.4 7.8 5.5 6.7 – 5.7 5.7 5.7 5.7 6.7 7.0 6.9 8.0 8.1 8.6 6.8 6.6 6.6
Std Deviation (1e-4) 1.2 1.2 1.7 2.0 2.1 2.2 5.0 – 1.7 1.6 1.7 1.5 1.3 1.2 1.4 1.6 1.7 1.9 1.7 1.7 1.8
Intensity Change (Log2) – – – -0.07 -0.0 -0.51 -0.22 – -0.44 -0.44 -0.45 -0.45 -0.21 -0.17 -0.18 0.05 0.05 0.14 -0.19 -0.24 -0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 3.2 0 – 3.0 0 0 2.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 -0.6 -0.5 0.3 – -0.4 -1.4 -0.9 -1.2 -0.5 1.6 1.2 0 -0.1 1.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome -0.2 -0.7 -4.2 -1.5 – -2.6 -4.7 -4.0 -3.7 2.0 2.7 2.4 2.4 2.1 1.3
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.1 0 – 0 0 3.2 2.8 0 0 0 3.1 3.5 0
Nucleus 0 0 0.9 0 – 1.1 0 1.3 0 0 0 0 1.5 0.9 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.1 0.5 -0.3 -1.5 – 0.5 0.5 1.2 -1.2 5.2 4.1 2.9 2.1 1.6 2.7
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.1 0.7 -1.3 -1.0 – -1.7 -1.0 -3.2 -0.3 -2.7 -4.7 -4.6 -4.6 -4.5 -4.3
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 2.1 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4445 2.1307 1.8846 1.5953 1.7594 1.6664 3.6805 3.7243 3.6121 3.8425 3.8805 3.7693 5.4931 7.1805 6.075 5.8856 6.0261 5.7488
Actin 0.0124 0 0.0004 0.0044 0.0008 0.0007 0.0285 0 0.0028 0.0001 0.0143 0.0001 0.0216 0 0 0.0001 0.0001 0
Bud 0.0002 0.0001 0.0001 0.0002 0.0006 0.0001 0.0004 0.0002 0.0002 0 0.0003 0 0 0.0001 0 0.0001 0 0
Bud Neck 0.0049 0.001 0.0004 0.0003 0.0004 0.0009 0.0008 0.0013 0.0006 0.0002 0.0008 0.0011 0.0005 0.0012 0.0009 0.0134 0.0004 0.0004
Bud Periphery 0.0002 0 0.0001 0.0003 0.0012 0.0002 0.0004 0.0002 0.0001 0 0.0008 0 0 0.0001 0 0 0 0
Bud Site 0.0051 0.0018 0.0004 0.0004 0.0012 0.001 0.0018 0.0043 0.004 0.0001 0.0008 0.0002 0.0002 0.0016 0.0005 0.0023 0.0002 0.0001
Cell Periphery 0.0003 0 0 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0 0 0 0 0
Cytoplasm 0.0026 0.0003 0.0082 0.0002 0.0008 0.0001 0.0045 0.0065 0.0038 0.0001 0.0003 0.001 0.0003 0.0173 0.0002 0.0001 0.0005 0.0001
Cytoplasmic Foci 0.0241 0.0229 0.0101 0.0417 0.0301 0.0089 0.0277 0.0057 0.0142 0.0153 0.0788 0.0051 0.0252 0.0288 0.0149 0.0304 0.0838 0.0033
Eisosomes 0.0003 0 0 0.0001 0 0 0.0002 0 0.0001 0 0.0002 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0013 0 0.0001 0.0002 0.0024 0 0.0041 0.0001 0.0002 0 0.0009 0 0.0003 0.0001 0 0 0.0001 0
Endosome 0.0161 0.0004 0.0012 0.0084 0.0376 0.0005 0.0193 0.0002 0.0043 0.0002 0.0195 0.0006 0.0017 0.01 0.0008 0.0006 0.0035 0.0001
Golgi 0.01 0.0003 0.0018 0.0102 0.0057 0.0006 0.0072 0.0003 0.0042 0.0003 0.0234 0.0006 0.0011 0.0005 0.0003 0.001 0.0023 0.0001
Lipid Particles 0.0268 0.0038 0.0102 0.0313 0.0428 0.0031 0.034 0.0006 0.0176 0.0008 0.082 0.0039 0.0031 0.0013 0.002 0.0018 0.0478 0.0003
Mitochondria 0.0034 0 0.0139 0.0148 0.0098 0.0076 0.0069 0 0.0048 0 0.0348 0 0.0002 0 0 0 0.0001 0
None 0.002 0.0001 0.0001 0.0002 0.0004 0.0001 0.003 0.0035 0.0003 0 0.0005 0.0124 0.0009 0.0001 0.0002 0.0001 0.0004 0
Nuclear Periphery 0.0093 0.0002 0.0002 0.0008 0.0291 0.0002 0.0152 0.0004 0.0006 0.0002 0.0054 0.0002 0.001 0.0004 0.0003 0.0002 0.0025 0.0001
Nucleolus 0.0058 0.0336 0.0276 0.0021 0.0057 0.0152 0.0191 0.054 0.0221 0.004 0.0093 0.0176 0.0092 0.0796 0.0215 0.0163 0.0137 0.0134
Nucleus 0.0047 0.0357 0.0043 0.0015 0.0034 0.0045 0.0094 0.0604 0.0289 0.0015 0.0014 0.0177 0.0085 0.0388 0.0097 0.0027 0.006 0.0097
Peroxisomes 0.0155 0.0004 0.0022 0.0046 0.0062 0.001 0.0107 0.0004 0.008 0.0009 0.0329 0.0011 0.0012 0.001 0.0003 0.0022 0.0061 0.0001
Punctate Nuclear 0.8537 0.8992 0.9185 0.8775 0.803 0.955 0.8024 0.8618 0.8827 0.9763 0.6903 0.9382 0.9249 0.814 0.9484 0.9287 0.8319 0.9721
Vacuole 0.0007 0.0001 0.0001 0.0005 0.0078 0.0001 0.0034 0.0002 0.0003 0 0.0009 0 0 0.0047 0.0001 0 0.0003 0.0002
Vacuole Periphery 0.0008 0 0.0001 0.0006 0.011 0 0.001 0 0.0001 0 0.0021 0 0.0001 0.0002 0 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.7291 4.5455 17.9892 11.7252 19.778 20.328 22.5968 12.9249 18.3509 18.653
Translational Efficiency 0.8691 3.967 0.8212 1.3249 0.8974 1.0282 1.0602 1.4904 1.1869 1.1125

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
173 781 405 2277 1988 2710 177 1961 2161 3491 582 4238

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 709.77 791.22 1084.65 1009.07 780.79 891.71 1116.42 1008.62 775.10 869.23 1094.31 1008.86
Standard Deviation 91.20 125.64 122.79 290.16 101.18 116.91 130.47 129.47 102.25 126.08 126.03 230.20
Intensity Change Log 2 — 0.156727 0.611806 0.507603 — 0.191640 0.515873 0.369376 — 0.175121 0.562351 0.436850

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000392 0.001415 0.000332 0.000125 0.000373 0.000427 0.000408 0.000128 0.000374 0.000648 0.000355 0.000127
Bud Neck 0.009089 0.007403 0.001751 0.001944 0.004397 0.016922 0.001904 0.001651 0.004773 0.014792 0.001797 0.001808
Bud Site 0.007491 0.016933 0.002190 0.007901 0.004552 0.011402 0.003368 0.005106 0.004788 0.012640 0.002548 0.006608
Cell Periphery 0.000227 0.000483 0.000037 0.000053 0.000163 0.000299 0.000055 0.000025 0.000169 0.000340 0.000043 0.000040
Cytoplasm 0.011021 0.054080 0.003010 0.008960 0.009531 0.056364 0.000602 0.013808 0.009650 0.055853 0.002278 0.011204
Cytoplasmic Foci 0.054146 0.092620 0.003747 0.008766 0.030995 0.042118 0.006465 0.004790 0.032848 0.053416 0.004574 0.006927
Eisosomes 0.000149 0.000135 0.000021 0.000006 0.000108 0.000063 0.000032 0.000005 0.000111 0.000079 0.000024 0.000006
Endoplasmic Reticulum 0.002394 0.003781 0.001704 0.000151 0.001857 0.001486 0.001080 0.000237 0.001900 0.001999 0.001514 0.000191
Endosome 0.019961 0.015792 0.001097 0.002795 0.005856 0.009682 0.000845 0.001477 0.006985 0.011049 0.001021 0.002185
Golgi 0.011905 0.019133 0.000525 0.001052 0.008598 0.006300 0.001170 0.002409 0.008863 0.009171 0.000722 0.001680
Lipid Particles 0.010674 0.012454 0.000556 0.001668 0.009216 0.002763 0.000778 0.000339 0.009333 0.004931 0.000623 0.001053
Mitochondria 0.004548 0.002058 0.000146 0.000661 0.000881 0.007832 0.000062 0.000309 0.001174 0.006540 0.000120 0.000498
Mitotic Spindle 0.592668* 0.454117* 0.930337* 0.945207* 0.614731* 0.461771* 0.914880* 0.949847* 0.612965* 0.460058* 0.925636* 0.947354*
None 0.006021 0.009858 0.000237 0.001652 0.008744 0.022001 0.000207 0.000916 0.008526 0.019284 0.000228 0.001312
Nuclear Periphery 0.000521 0.000492 0.000274 0.000274 0.000535 0.000802 0.000250 0.000417 0.000534 0.000733 0.000267 0.000340
Nuclear Periphery Foci 0.099537 0.151246 0.007414 0.006112 0.127608 0.037663 0.030916 0.003789 0.125361 0.063074 0.014562 0.005037
Nucleolus 0.084597 0.069976 0.008557 0.002272 0.116263 0.163141 0.015794 0.003029 0.113728 0.142298 0.010758 0.002622
Nucleus 0.035649 0.037953 0.029878 0.004112 0.037959 0.145562* 0.013537 0.007241 0.037774 0.121488 0.024908 0.005560
Peroxisomes 0.046245 0.042714 0.002385 0.001744 0.016640 0.008789 0.005525 0.001311 0.019010 0.016379 0.003340 0.001544
Vacuole 0.002410 0.006076 0.005719 0.003716 0.000825 0.003735 0.002038 0.002749 0.000952 0.004259 0.004600 0.003268
Vacuole Periphery 0.000355 0.001281 0.000084 0.000826 0.000167 0.000878 0.000083 0.000416 0.000183 0.000968 0.000083 0.000636

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.83 -4.96 2.93 8.51 6.04 -0.48 -3.11 0.02 0.43 3.10 -5.99 -6.50 0.99 7.07 6.73
Bud Neck -0.21 -1.18 1.36 3.97 4.00 -11.54 -3.96 -5.11 7.06 1.68 -11.69 -6.03 -4.33 8.94 4.11
Bud Site -4.79 -2.42 -3.15 2.25 -0.76 -8.77 -4.30 -7.75 -2.46 0.26 -10.98 -6.94 -9.95 -1.38 -0.57
Cell Periphery -3.81 3.63 5.81 9.01 3.63 -3.65 -0.13 11.53 12.15 5.48 -6.27 1.46 10.02 13.72 6.43
Cytoplasm -4.75 0.63 -1.67 4.38 -4.20 -10.05 1.80 -5.83 2.71 -6.40 -11.64 0.69 -7.07 5.28 -6.96
Cytoplasmic Foci -2.76 5.95 4.90 11.89 -4.92 -3.53 6.37 9.78 13.48 0.13 -5.76 11.82 10.37 17.31 -3.52
Eisosomes 3.36 7.07 10.37 19.10 7.00 15.88 9.96 33.03 28.48 5.93 14.04 13.79 34.88 34.31 8.96
Endoplasmic Reticulum -1.52 -1.27 9.01 14.99 6.61 9.97 -0.40 17.20 9.35 3.39 4.31 -2.96 22.64 18.37 7.36
Endosome -0.07 2.62 2.85 7.97 1.18 -2.19 5.55 6.76 7.93 0.59 -3.43 4.58 6.87 10.74 1.56
Golgi -2.88 7.09 7.51 14.33 2.61 8.15 14.14 12.30 7.09 1.36 3.60 19.51 18.66 16.54 1.82
Lipid Particles 0.50 5.06 5.00 6.29 0.00 7.15 7.78 9.51 6.46 4.20 4.56 8.68 9.24 6.69 1.69
Mitochondria 0.68 1.39 1.26 1.77 -1.11 -3.56 4.19 1.07 3.69 -2.06 -3.55 1.97 0.83 3.79 -1.42
Mitotic Spindle 1.60 -8.24 -12.51 -27.87* -5.73 0.76 -10.18 -33.51* -37.12* -3.92 2.11 -16.03 -38.00 -47.37* -6.95
None -0.87 2.98 2.64 6.78 -0.88 -5.20 8.56 7.18 11.73 -3.04 -5.08 8.97 7.44 12.85 -2.01
Nuclear Periphery 1.37 -3.15 -0.30 -1.06 2.01 -5.36 -2.29 -0.20 1.33 1.49 -4.25 -4.61 -0.65 0.78 2.67
Nuclear Periphery Foci -0.39 8.23 9.07 17.70* 2.20 18.36* 12.46 31.78* 20.03* 4.95 14.83 22.56 32.31 25.03* 5.24
Nucleolus 2.24 4.48 7.17 13.10 6.59 -6.79 10.10 23.95* 31.76* 4.24 -4.20 14.35 25.91 34.34* 7.66
Nucleus -0.52 -3.66 2.03 4.97 5.78 -13.42 -1.31 1.64 14.14 1.79 -12.81 -4.79 3.62 16.13 5.89
Peroxisomes -0.10 5.14 5.61 12.99 2.53 7.78 4.59 14.91 11.26 4.93 2.68 9.34 15.49 16.24 4.87
Vacuole -3.52 -4.82 -4.33 -1.80 3.35 -4.36 -3.84 -5.99 -4.91 0.38 -6.85 -5.91 -8.40 -5.95 3.33
Vacuole Periphery -1.66 -0.50 -1.55 0.59 -1.41 -6.87 -4.09 -2.05 0.80 -0.39 -5.16 -6.33 -3.25 0.28 -1.34
Standard name
Human Ortholog
Description Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND)
Localization
Cell Percentages spindle pole (79%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nsl1

Nsl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nsl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available