ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0 0 0 0 0 0 0
Cytoplasm 0.98 0.96 0.97 0.99 0.94 0.82 0.72 0.71 0.65 0.63 0.6 0.6 1.0 0.99 0.99 0.97 1.0 0.99 0.95 0.93 0.92 0.87 0.86 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0.14 0.38 0.47 0.38 0.54 0.47 0.56 0.55 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 2
Bud 0 2 2 2 4 7 13 13 16 16 24 14 0 0 0 0 0 0 0 1 0 1 3 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Bud Site 0 0 0 0 0 0 2 1 1 2 4 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 2 4 7 4 9 5 12 23 10 14 1 1 10 7 13 5 0 0 0 0 0 0
Cytoplasm 214 183 260 316 519 590 602 680 448 549 418 369 243 378 454 145 151 142 222 179 246 252 247 313
Endoplasmic Reticulum 1 0 3 0 2 4 0 4 2 6 6 14 0 2 2 6 4 3 1 0 2 7 5 7
Endosome 1 0 1 2 6 4 5 8 5 5 1 2 0 1 0 0 0 0 1 2 2 1 3 1
Golgi 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 2 3
Mitochondria 1 4 11 22 75 277 396 371 371 410 386 337 0 0 0 3 0 1 2 3 5 6 10 11
Nucleus 0 0 0 0 0 2 1 11 3 7 1 7 1 0 1 0 0 2 0 0 1 0 0 1
Nuclear Periphery 0 0 0 0 0 3 2 8 0 5 3 1 0 0 0 0 0 0 0 0 1 1 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Vac/Vac Membrane 1 0 0 3 3 11 12 64 50 60 26 13 0 0 1 0 0 1 1 0 0 7 7 9
Unique Cell Count 218 190 267 320 551 721 836 964 684 878 692 612 243 380 457 149 151 144 233 194 267 290 288 369
Labelled Cell Count 219 192 280 349 617 902 1042 1167 908 1083 879 771 245 383 468 161 168 154 233 194 267 290 288 369


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 6.1 5.0 4.9 4.8 4.5 4.0 4.5 4.1 4.2 4.3 4.2 6.8 6.7 6.8 6.5 6.8 6.8 7.6 7.3 7.7
Std Deviation (1e-4) 0.6 0.8 0.8 1.2 1.2 1.1 0.9 1.1 0.9 0.8 1.2 1.1 1.2 1.2 1.0 1.0 1.1 1.0 1.6 1.5 1.6
Intensity Change (Log2) – – – -0.02 -0.06 -0.15 -0.33 -0.16 -0.28 -0.24 -0.21 -0.26 0.45 0.42 0.46 0.39 0.44 0.44 0.6 0.56 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 4.2 0
Cytoplasm 1.2 -2.0 -6.3 -8.7 -9.1 -10.1 -11.0 -11.3 -11.2 2.5 2.2 2.2 0 2.0 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.4 0 0 0 0 0 0 0 0 -3.2 0 0 -1.1 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.3 4.5 4.4 3.2 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7128 2.1046 1.807 1.2734 1.2691 1.9556 2.4936 2.9888 2.7855 2.5163 2.2222 2.9156 0.4488 0.9446 0.7877 0.6849 0.4071 0.5823
Actin 0.0029 0.0026 0.0012 0.0002 0.0003 0.0008 0.0117 0.0004 0.0014 0.0101 0.0034 0.0035 0.0315 0.0008 0.0046 0.0001 0.0001 0.013
Bud 0.0001 0.0009 0.0006 0.0012 0.0002 0.0019 0.0007 0.0001 0.0003 0.0035 0.0003 0.0007 0.0002 0.0001 0.0002 0.0001 0 0.0001
Bud Neck 0.0013 0.0004 0.0059 0.0004 0.0004 0.0008 0.0039 0.0001 0.0002 0.0003 0.0005 0.0007 0.0018 0.0001 0.0001 0.0001 0.0001 0.0004
Bud Periphery 0.0001 0.0004 0.0008 0.0011 0.0001 0.0014 0.0012 0.0001 0.0003 0.0029 0.0002 0.0008 0.0002 0.0001 0.0002 0 0 0.0001
Bud Site 0.003 0.0097 0.0023 0.0003 0.0002 0.0003 0.0011 0.0008 0.0012 0.0006 0.0002 0.0002 0.0016 0.0003 0.0008 0.0001 0 0.0001
Cell Periphery 0.0002 0.0002 0.0001 0 0 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0 0 0 0
Cytoplasm 0.4712 0.5391 0.5048 0.6779 0.5491 0.6103 0.6276 0.7436 0.6592 0.7831 0.6974 0.7354 0.5532 0.7728 0.7442 0.7437 0.906 0.8731
Cytoplasmic Foci 0.0234 0.0154 0.0226 0.0123 0.0154 0.0159 0.0119 0.0073 0.0112 0.0154 0.0101 0.0109 0.0332 0.0036 0.0081 0.0094 0.0062 0.0046
Eisosomes 0.0005 0.0001 0.0001 0 0 0.0001 0.0002 0 0.0001 0 0.0001 0 0.0005 0.0001 0 0 0 0.0001
Endoplasmic Reticulum 0.0051 0.0033 0.0023 0.0036 0.0017 0.0016 0.0063 0.0027 0.0037 0.0026 0.0029 0.0028 0.0053 0.0045 0.0026 0.0027 0.0033 0.0023
Endosome 0.0084 0.0094 0.0036 0.0143 0.0109 0.0049 0.0094 0.0028 0.0048 0.0071 0.0056 0.0051 0.0175 0.0027 0.0051 0.0073 0.0046 0.0043
Golgi 0.001 0.0012 0.0005 0.0003 0.0002 0.0002 0.0012 0.0002 0.0006 0.0018 0.0004 0.0004 0.0103 0.0007 0.007 0.0002 0.0001 0.0086
Lipid Particles 0.0015 0.0006 0.0002 0.0001 0 0.0002 0.0014 0.0001 0.0003 0.0001 0.0001 0.0003 0.0109 0.0009 0.002 0.0001 0 0.0029
Mitochondria 0.0006 0.0009 0.0005 0.0007 0.0006 0.0006 0.0005 0.0002 0.0004 0.0006 0.0003 0.0004 0.0018 0.0006 0.0044 0.0001 0.0001 0.0022
None 0.4695 0.4034 0.4446 0.2743 0.4108 0.3528 0.3119 0.2378 0.3097 0.1628 0.2747 0.2347 0.3196 0.2101 0.1978 0.2322 0.0746 0.0839
Nuclear Periphery 0.0021 0.0013 0.0009 0.0019 0.0011 0.0007 0.0024 0.0007 0.0011 0.0013 0.0006 0.0007 0.0013 0.0007 0.0006 0.0008 0.001 0.0005
Nucleolus 0.0006 0.0004 0.0003 0.0001 0.0001 0.0004 0.0003 0.0001 0.0002 0.0001 0 0.0001 0.0001 0 0 0 0 0
Nucleus 0.0021 0.0044 0.0031 0.0036 0.0038 0.0029 0.0021 0.0013 0.002 0.0021 0.0012 0.0013 0.0006 0.0006 0.0006 0.0012 0.0017 0.0017
Peroxisomes 0.0021 0.0009 0.001 0.0001 0.0002 0.0003 0.0007 0.0001 0.0002 0.0013 0.0001 0.0002 0.0082 0.0001 0.0202 0.0001 0 0.0011
Punctate Nuclear 0.0017 0.003 0.0033 0.0012 0.0022 0.0023 0.003 0.0005 0.0013 0.0029 0.0006 0.0009 0.0011 0.0002 0.0003 0.0004 0.0006 0.0003
Vacuole 0.0023 0.002 0.0012 0.0051 0.0021 0.0013 0.002 0.0011 0.0013 0.0011 0.001 0.0007 0.001 0.0007 0.0009 0.0013 0.0013 0.0004
Vacuole Periphery 0.0003 0.0003 0.0002 0.0012 0.0005 0.0003 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0003 0.0001 0.0002 0.0002 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.5536 4.7651 5.8932 8.3807 8.063 3.5517 9.2345 9.2135 4.8094 9.9188
Translational Efficiency 6.6329 3.0587 2.3851 1.0124 2.2102 7.6174 1.8724 1.8307 3.962 2.3946

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1108 1410 1774 1148 1895 1425 1842 1586 3003 2835 3616 2734

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 729.17 840.95 911.86 952.17 775.80 780.30 938.92 972.39 758.60 810.46 925.64 963.90
Standard Deviation 230.41 118.68 118.60 141.82 85.82 105.94 110.26 134.69 157.30 116.47 115.23 138.09
Intensity Change Log 2 — 0.205765 0.322557 0.384964 — 0.008344 0.275317 0.325850 — 0.107372 0.298399 0.354794

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000090 0.000283 0.000211 0.002525 0.000117 0.000326 0.000227 0.000633 0.000107 0.000305 0.000219 0.001428
Bud Neck 0.008526 0.020079 0.002009 0.003846 0.007795 0.021663 0.002003 0.003677 0.008065 0.020875 0.002006 0.003748
Bud Site 0.004789 0.019973 0.005124 0.043109 0.003816 0.019420 0.009842 0.021762 0.004175 0.019695 0.007527 0.030725
Cell Periphery 0.000130 0.000290 0.000076 0.000124 0.000131 0.000130 0.000071 0.000081 0.000131 0.000210 0.000073 0.000099
Cytoplasm 0.539050* 0.540732* 0.650873* 0.663234* 0.549326* 0.476488* 0.723317* 0.684324* 0.545534* 0.508440* 0.687776* 0.675468*
Cytoplasmic Foci 0.235557* 0.192738* 0.012866 0.032309 0.248147* 0.272424* 0.018977 0.051619 0.243502* 0.232792* 0.015979 0.043511
Eisosomes 0.000111 0.000076 0.000016 0.000028 0.000126 0.000131 0.000019 0.000035 0.000120 0.000104 0.000017 0.000032
Endoplasmic Reticulum 0.001171 0.000845 0.002006 0.001128 0.001819 0.001003 0.001048 0.000563 0.001580 0.000924 0.001518 0.000800
Endosome 0.017217 0.021044 0.001570 0.002485 0.014174 0.033388 0.001861 0.008057 0.015297 0.027249 0.001718 0.005717
Golgi 0.002278 0.004121 0.000082 0.004982 0.003645 0.009183 0.000128 0.003641 0.003140 0.006665 0.000105 0.004204
Lipid Particles 0.007183 0.003128 0.000745 0.000862 0.007339 0.005599 0.000688 0.000981 0.007281 0.004370 0.000716 0.000931
Mitochondria 0.001165 0.004757 0.000364 0.002566 0.003357 0.008082 0.000309 0.002811 0.002548 0.006428 0.000336 0.002708
Mitotic Spindle 0.000447 0.005574 0.002272 0.024609 0.001370 0.003954 0.008862 0.011848 0.001030 0.004760 0.005629 0.017206
None 0.003049 0.003078 0.005500 0.003160 0.001107 0.001066 0.003532 0.002044 0.001824 0.002067 0.004498 0.002513
Nuclear Periphery 0.000323 0.000280 0.000939 0.000405 0.000250 0.000176 0.000774 0.000298 0.000277 0.000228 0.000855 0.000343
Nuclear Periphery Foci 0.000660 0.000562 0.002262 0.002629 0.000467 0.000325 0.001683 0.001297 0.000538 0.000443 0.001967 0.001856
Nucleolus 0.000666 0.000763 0.000182 0.000409 0.000456 0.000579 0.000149 0.000178 0.000534 0.000671 0.000165 0.000275
Nucleus 0.095722 0.062009 0.265393* 0.066864 0.081596 0.033498 0.135921* 0.045171 0.086808 0.047678 0.199440* 0.054280
Peroxisomes 0.002197 0.005671 0.000193 0.005051 0.002728 0.007493 0.000223 0.003173 0.002532 0.006587 0.000208 0.003961
Vacuole 0.078527 0.111777 0.047200 0.137723 0.071068 0.101644 0.090252 0.155415 0.073820 0.106684 0.069131 0.147986
Vacuole Periphery 0.001143 0.002218 0.000118 0.001951 0.001167 0.003428 0.000113 0.002393 0.001158 0.002826 0.000116 0.002207

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.92 -6.02 -3.21 -2.64 -2.70 -6.94 -2.53 -3.55 -1.06 -2.16 -10.01 -5.31 -4.17 -2.86 -3.26
Bud Neck -6.38 6.06 5.61 10.82 -1.08 -7.72 8.67 7.11 10.73 -1.09 -10.21 10.32 9.11 15.19 -1.51
Bud Site -8.27 -0.42 -6.54 -0.93 -6.19 -11.07 -2.75 -6.44 2.57 -3.51 -13.76 -2.44 -9.20 1.08 -7.02
Cell Periphery -3.14 3.12 2.25 3.96 -1.69 0.08 2.62 2.95 9.22 1.08 -2.68 3.42 3.44 5.42 -0.00
Cytoplasm 0.30 -5.84 -1.67 -1.95 3.66 7.83 -8.55 -6.64 -13.13 1.12 5.65 -9.99 -5.98 -10.82 2.91
Cytoplasmic Foci 5.81 37.46 34.05 30.68 -10.35 -3.26 49.73 42.51 39.79 -12.46 2.35 62.49 54.72 48.75 -16.31
Eisosomes 5.84 17.81 15.32 11.31 -6.77 -0.55 16.06 14.46 13.54 -2.78 2.90 22.28 19.64 17.00 -5.92
Endoplasmic Reticulum 1.61 -6.04 0.96 -1.24 9.95 3.31 1.90 6.43 3.50 12.36 3.94 -2.40 5.89 2.59 14.91
Endosome -1.86 12.40 12.41 13.79 0.12 -10.26 15.18 9.68 16.31 -4.53 -9.07 19.56 15.53 20.79 -4.39
Golgi -2.56 5.03 0.53 2.01 -1.56 -6.85 10.30 3.35 8.58 -3.17 -6.59 11.28 2.55 7.22 -3.05
Lipid Particles 4.15 6.59 6.33 6.34 -2.73 1.93 12.85 12.76 6.08 0.60 4.72 13.49 13.26 7.98 -0.94
Mitochondria -3.70 3.83 0.30 3.46 -1.20 -3.22 3.89 2.31 5.16 -2.33 -4.14 4.42 2.30 6.18 -2.54
Mitotic Spindle -4.30 -4.60 -5.93 -3.08 -4.67 -2.00 -4.97 -5.04 -2.63 -0.11 -4.18 -5.93 -7.70 -3.95 -3.54
None -0.00 -1.98 0.55 0.76 3.47 0.09 -9.90 -4.07 -2.33 4.56 -0.56 -5.65 -1.53 -0.73 5.24
Nuclear Periphery 0.92 -18.01 -7.95 -9.07 10.66 3.05 -23.46 -7.00 -9.29 16.57 2.10 -29.56 -10.76 -12.46 19.39
Nuclear Periphery Foci 0.83 -9.06 -8.29 -9.10 0.74 3.00 -12.03 -7.21 -8.94 4.38 1.73 -14.04 -10.99 -12.13 3.34
Nucleolus -1.05 6.67 4.63 5.47 -0.92 -1.46 6.95 6.72 4.29 -0.16 -2.20 9.53 7.46 7.11 -0.85
Nucleus 7.46 -30.58 2.43 -5.45 33.93 18.50 -20.45 7.51 -11.15 26.06 15.97 -36.81 6.54 -9.80 42.16
Peroxisomes -4.82 9.02 0.33 3.59 -2.20 -6.05 8.20 1.94 6.63 -2.55 -7.47 10.99 1.68 7.26 -3.35
Vacuole -7.30 0.72 -22.38 -16.99 -22.95 -7.83 -16.93 -26.47 -20.06 -11.18 -11.17 -12.65 -34.78 -25.88 -24.17
Vacuole Periphery -4.22 8.76 1.33 3.84 -1.81 -7.04 12.39 0.19 4.78 -2.65 -8.18 15.12 0.88 5.88 -3.20
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (82%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YPL229W

YPL229W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypl229w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available