Standard name
Human Ortholog
Description Component of U2 snRNP complex; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.09 0 0.09 – 0.08 0.06 0.06 0 0.08 0.13 0.18 0.32 0.24 0.28 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.21 0 – 0.06 0.08 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.96 0.96 0.94 0.87 0.85 0.85 – 0.82 0.83 0.81 0.82 0.9 0.83 0.74 0.56 0.67 0.66 0.89 0.91 0.92 0.74 0.76 0.65
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0.06 0.05 – 0.19 0.13 0.16 0.19 0 0 0.05 0 0 0 0 0 0 0.05 0.06 0.07
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.08 0.05 0 0 0 0.12 0.13 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 1 1 1 2 0 1 0 0 1 0 3 1 1 1 3 3
Bud Neck 0 0 0 0 0 0 1 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 6 0 – 4 8 12 15 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 5 6 8 23 11 29 – 22 17 18 7 24 38 61 37 22 16 2 0 0 3 0 2
Endoplasmic Reticulum 0 0 1 0 0 0 0 – 0 0 0 1 0 0 0 3 2 1 1 0 0 1 0 1
Endosome 1 1 0 0 0 0 0 – 0 0 0 0 1 2 3 4 1 0 1 3 5 7 0 2
Golgi 0 0 0 0 2 0 0 – 0 0 0 0 0 1 0 0 1 0 2 1 2 2 0 1
Mitochondria 2 5 1 0 0 51 9 – 16 22 19 32 2 2 1 5 3 1 3 1 0 0 5 3
Nucleus 316 214 255 138 213 209 262 – 226 241 244 297 279 236 248 65 60 38 312 220 253 206 233 173
Nuclear Periphery 6 0 0 0 3 1 0 – 1 3 3 7 3 1 3 3 3 1 0 0 0 2 2 3
Nucleolus 24 9 3 2 4 15 14 – 53 38 47 70 2 8 16 3 1 1 10 3 4 13 16 18
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 1 0 4 0 0 0 1 0 0 0 1 0
SpindlePole 0 0 1 0 1 1 2 – 0 0 0 0 2 1 8 0 0 0 0 0 0 2 0 1
Vac/Vac Membrane 6 2 5 1 5 1 6 – 4 3 2 6 2 3 4 9 7 3 12 4 4 33 39 51
Unique Cell Count 336 224 265 147 245 246 308 274 291 302 363 310 283 333 117 90 58 352 242 275 280 307 268
Labelled Cell Count 357 236 272 149 251 295 323 327 333 347 437 316 293 350 129 101 61 352 242 275 280 307 268


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 8.9 9.0 8.3 9.3 5.7 7.9 – 7.3 7.2 7.1 7.1 8.4 9.3 8.9 10.2 10.4 10.0 8.0 8.3 8.3
Std Deviation (1e-4) 1.9 2.8 1.8 1.8 2.1 1.5 2.0 – 2.0 1.7 1.8 1.6 2.4 2.8 2.9 2.7 3.0 2.9 2.0 1.9 2.1
Intensity Change (Log2) – – – -0.12 0.05 -0.66 -0.19 – -0.3 -0.32 -0.35 -0.33 -0.1 0.05 -0.02 0.19 0.22 0.16 -0.17 -0.11 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 3.3 3.3 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 3.5 1.4 3.6 – 3.0 2.1 2.2 -0.3 2.9 4.8 6.2 8.4 6.7 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 7.6 0 – 3.6 4.2 3.8 4.7 0 0 0 0 0 0
Nucleus -1.1 -3.8 -4.4 -4.5 – -5.1 -5.1 -5.6 -5.5 -2.9 -4.9 -7.2 -9.9 -7.7 -7.3
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.0 2.4 – 6.9 5.4 6.0 7.0 0 1.4 2.5 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0.1 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2738 2.1982 2.0519 1.5106 1.6436 1.8936 3.8203 3.84 3.5091 3.4066 3.0813 3.564 6.066 6.2719 5.8574 6.1681 5.2134 5.9125
Actin 0 0 0 0 0 0 0.0112 0.0027 0.0126 0.0104 0.0033 0.0012 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.0004 0 0.0002 0.0001 0.0002 0.0001 0 0 0 0 0 0
Bud Neck 0 0 0 0.0001 0.0002 0.0004 0.0017 0.0001 0.0007 0.0002 0.0005 0.0018 0 0 0 0 0.0001 0.0004
Bud Periphery 0 0 0 0 0 0 0.0006 0.0001 0.0003 0.0001 0.0008 0.0002 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.0009 0.0006 0.0037 0.0002 0.0003 0.0005 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.0001 0 0.0001 0 0.0001 0.0001 0 0 0 0 0 0
Cytoplasm 0 0 0.0002 0.0001 0 0 0.003 0.0004 0.0003 0.0001 0.0003 0.0028 0.0002 0.0001 0.0001 0.0001 0 0
Cytoplasmic Foci 0 0 0 0 0 0 0.0116 0.001 0.003 0.0015 0.0166 0.0003 0 0 0 0 0.0014 0
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0001 0.0003 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.0031 0.0001 0.0005 0.0002 0.0004 0.0005 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0.0172 0.0008 0.0028 0.0052 0.0043 0.0038 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.0069 0.0002 0.0032 0.0069 0.005 0.0006 0 0 0 0 0.0001 0
Lipid Particles 0 0 0 0 0 0 0.0232 0.0009 0.0023 0.0286 0.0309 0.0004 0 0 0 0 0.0145 0
Mitochondria 0.0001 0 0 0.0001 0.0002 0 0.0044 0.002 0.0033 0.0108 0.0087 0.0014 0.0001 0.0001 0 0 0.0002 0
None 0 0 0.0005 0 0 0 0.001 0.0026 0.0002 0.0001 0.0003 0.004 0.0001 0 0 0 0 0
Nuclear Periphery 0.0012 0.0023 0.0019 0.0034 0.0014 0.0004 0.0154 0.0021 0.0075 0.0022 0.0246 0.0068 0.0007 0.0015 0.0007 0.0005 0.0008 0.0006
Nucleolus 0.0119 0.0115 0.007 0.0038 0.0408 0.0372 0.011 0.0056 0.0056 0.0052 0.0413 0.0319 0.0114 0.0101 0.0131 0.0055 0.029 0.0256
Nucleus 0.9862 0.9851 0.9846 0.9918 0.9556 0.9588 0.866 0.9742 0.9421 0.9237 0.8187 0.937 0.9846 0.9852 0.9842 0.9922 0.9504 0.972
Peroxisomes 0 0 0 0 0 0 0.014 0.002 0.0062 0.0027 0.0329 0.0001 0 0 0 0 0.0004 0
Punctate Nuclear 0.0004 0.0009 0.0058 0.0003 0.0015 0.0029 0.0059 0.0042 0.0043 0.0007 0.0098 0.0056 0.0028 0.0029 0.0018 0.0017 0.0028 0.0012
Vacuole 0 0 0 0.0001 0.0001 0 0.0013 0.0003 0.0003 0.0004 0.0003 0.0006 0 0 0 0 0 0
Vacuole Periphery 0 0 0 0.0001 0.0001 0 0.0009 0.0001 0.0005 0.0006 0.0008 0.0004 0 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.7724 5.5028 12.2499 14.1715 14.9646 3.0761 12.5187 18.3068 10.7811 12.4361
Translational Efficiency 1.8975 2.8709 1.9398 1.5845 1.227 7.6995 2.0269 1.1625 2.2559 1.8854

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1689 833 84 1377 1660 2601 1868 1616 3349 3434 1952 2993

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 901.89 842.05 1049.62 979.76 865.50 982.98 980.34 1093.30 883.85 948.79 983.32 1041.06
Standard Deviation 143.05 180.84 134.43 162.07 126.80 158.67 151.09 254.10 136.46 175.07 151.07 223.93
Intensity Change Log 2 — -0.099046 0.218844 0.119477 — 0.183628 0.179748 0.337084 — 0.046300 0.199831 0.230141

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000409 0.000886 0.001286 0.001240 0.000271 0.000349 0.000470 0.000962 0.000341 0.000480 0.000505 0.001090
Bud Neck 0.005849 0.006159 0.003065 0.028498 0.003791 0.010134 0.014476 0.035235 0.004828 0.009170 0.013985 0.032135
Bud Site 0.001088 0.001993 0.000712 0.008953 0.000314 0.001018 0.000590 0.008677 0.000704 0.001255 0.000595 0.008804
Cell Periphery 0.000224 0.000368 0.000061 0.000156 0.000111 0.000232 0.000046 0.000336 0.000168 0.000265 0.000047 0.000253
Cytoplasm 0.000583 0.004762 0.002000 0.007944 0.000193 0.000495 0.000582 0.007971 0.000390 0.001530 0.000643 0.007959
Cytoplasmic Foci 0.000073 0.001316 0.000306 0.002472 0.000154 0.000213 0.000007 0.003052 0.000113 0.000480 0.000020 0.002785
Eisosomes 0.000042 0.000035 0.000062 0.000030 0.000031 0.000049 0.000023 0.000112 0.000037 0.000046 0.000025 0.000074
Endoplasmic Reticulum 0.006583 0.006075 0.006790 0.005339 0.005558 0.006742 0.002704 0.007367 0.006075 0.006580 0.002879 0.006434
Endosome 0.000185 0.000932 0.000235 0.003034 0.000130 0.000549 0.000075 0.003400 0.000158 0.000642 0.000082 0.003231
Golgi 0.000077 0.000587 0.000109 0.001280 0.000076 0.000180 0.000041 0.002828 0.000076 0.000279 0.000043 0.002116
Lipid Particles 0.000278 0.000203 0.000525 0.000833 0.000068 0.000065 0.000016 0.000165 0.000174 0.000098 0.000038 0.000472
Mitochondria 0.001701 0.002293 0.000875 0.003894 0.000505 0.001244 0.000897 0.005201 0.001108 0.001498 0.000896 0.004599
Mitotic Spindle 0.000333 0.000668 0.000273 0.025275 0.000835 0.000731 0.000412 0.021536 0.000582 0.000716 0.000406 0.023256
None 0.002299 0.011105 0.001610 0.007600 0.001708 0.002021 0.001195 0.002375 0.002006 0.004225 0.001213 0.004779
Nuclear Periphery 0.000290 0.000222 0.000364 0.002230 0.000172 0.000428 0.000312 0.005340 0.000231 0.000378 0.000315 0.003909
Nuclear Periphery Foci 0.000394 0.000665 0.000202 0.001403 0.000106 0.000226 0.000070 0.002560 0.000251 0.000332 0.000076 0.002028
Nucleolus 0.014819 0.021213 0.022985 0.010609 0.013242 0.012611 0.009656 0.007723 0.014037 0.014697 0.010230 0.009050
Nucleus 0.963771* 0.938151* 0.955646* 0.879546* 0.971849* 0.960888* 0.967786* 0.871665* 0.967775* 0.955373* 0.967263* 0.875291*
Peroxisomes 0.000289 0.001005 0.000732 0.000950 0.000330 0.000210 0.000124 0.000329 0.000309 0.000403 0.000150 0.000614
Vacuole 0.000476 0.001037 0.002025 0.007291 0.000412 0.000941 0.000374 0.008189 0.000445 0.000964 0.000445 0.007776
Vacuole Periphery 0.000236 0.000325 0.000138 0.001423 0.000144 0.000673 0.000143 0.004980 0.000190 0.000589 0.000143 0.003343

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.97 -6.12 -7.67 -3.43 0.74 -2.24 -8.37 -13.62 -12.90 -10.30 -2.69 -4.56 -12.95 -12.56 -12.24
Bud Neck -0.43 3.36 -14.26 -14.05 -15.73 -8.71 -15.98 -19.36 -14.88 -11.58 -7.20 -14.27 -23.84 -19.16 -13.53
Bud Site -1.11 0.47 -4.94 -3.65 -5.47 -3.74 -5.38 -5.84 -5.26 -5.57 -1.79 0.15 -7.55 -6.91 -7.81
Cell Periphery -2.11 3.89 2.38 4.15 -4.16 -2.53 2.17 -1.24 -0.27 -1.79 -2.42 4.89 -0.51 0.83 -2.34
Cytoplasm -3.44 -1.50 -6.91 -1.73 -4.11 -1.74 -1.73 -6.66 -6.18 -5.89 -3.41 -1.30 -9.48 -7.20 -8.34
Cytoplasmic Foci -1.94 -1.68 -2.93 0.20 -2.34 0.26 1.70 -3.78 -3.93 -4.44 -1.67 1.93 -4.82 -3.33 -5.39
Eisosomes 1.59 -3.25 2.34 0.93 4.82 -4.81 4.00 -1.01 -0.70 -1.16 -2.82 5.41 -0.85 -0.56 -1.25
Endoplasmic Reticulum 0.99 -1.04 2.10 0.89 2.28 -2.82 9.52 -2.95 -1.09 -8.18 -1.50 13.16 -1.33 -0.08 -10.61
Endosome -1.96 -1.83 -3.76 -1.24 -3.54 -2.16 1.77 -4.90 -3.94 -5.17 -2.98 3.05 -6.18 -4.20 -6.51
Golgi -1.51 -1.41 -2.27 -0.30 -2.13 -0.69 1.09 -3.09 -2.94 -3.19 -1.65 1.41 -3.72 -3.04 -3.85
Lipid Particles 0.30 -0.75 -0.72 -1.37 -0.12 0.78 1.93 -1.48 -4.09 -7.47 0.75 1.24 -0.84 -2.23 -2.86
Mitochondria -0.42 0.96 -2.53 -1.17 -8.78 -2.97 -5.29 -10.11 -7.68 -9.16 -0.77 0.33 -7.25 -6.89 -12.45
Mitotic Spindle -0.83 0.11 -6.91 -6.73 -6.91 0.18 0.45 -6.95 -7.10 -7.16 -0.38 0.07 -9.80 -9.78 -9.84
None -4.94 0.87 -3.84 2.60 -4.88 -0.42 0.67 -1.18 -0.77 -3.77 -3.25 1.54 -3.77 0.07 -6.54
Nuclear Periphery 1.49 -1.46 -6.06 -6.24 -5.56 -5.42 -5.59 -6.22 -5.88 -5.96 -3.84 -4.07 -7.99 -7.64 -7.67
Nuclear Periphery Foci -1.32 1.01 -2.48 -1.10 -3.37 -0.05 1.10 -3.05 -3.02 -3.39 -0.32 2.14 -3.79 -3.57 -4.64
Nucleolus -3.29 -1.30 1.90 4.49 1.73 0.74 2.06 2.43 1.96 0.59 -0.64 2.48 3.10 3.66 0.50
Nucleus 5.43 1.48 12.60 6.78 6.87 4.02 3.19 16.44 14.66 15.48 5.49 2.03 20.57 17.08 19.63
Peroxisomes -2.14 -3.11 -1.88 1.23 0.83 1.18 1.67 0.70 -0.99 -2.44 -0.53 2.02 -0.72 -0.11 -3.55
Vacuole -2.87 -4.53 -5.72 -5.20 -3.92 -1.04 0.72 -7.42 -7.34 -7.91 -2.51 0.16 -9.21 -8.73 -9.32
Vacuole Periphery -1.39 1.72 -2.53 -2.17 -2.91 -3.72 -0.06 -4.77 -3.97 -4.76 -3.62 1.01 -5.28 -4.16 -5.37
Standard name
Human Ortholog
Description Component of U2 snRNP complex; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Lea1

Lea1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lea1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available