Standard name
Human Ortholog
Description tRNA:pseudouridine synthase; introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; also acts on U2 snRNA; also pseudouridylates some mRNAs, and pseudouridylation level varies with growth phase; nuclear protein that appears to be involved in tRNA export; PUS1 has a paralog, PUS2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.06 0 0 0 0.08 0 0.06 0.13 0.14 0.13 0.07 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.07 0.05 0.09 0.13 0.13 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.98 0.92 0.92 0.89 0.85 0.83 0.81 0.77 0.73 0.77 0.8 0.9 0.8 0.76 0.82 0.89 0.87 0.92 0.95 0.91 0.74 0.51 0.44
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.07 0.09 0.17 0.14 0.16 0.28 0.31 0.36 0.31 0 0.12 0.22 0 0 0 0 0 0 0.06 0.17 0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.24 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 0 0 0 1 3 1 0 1 0 0 0 0 0 2 2 1 0 0 0
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 0 1 2 5 6 7 9 1 4 1 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 4 11 7 8 7 17 8 5 5 8 5 9 16 12 40 19 13 0 0 0 0 0 0
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 5 3 2 0 1 0 0 0 0
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Mitochondria 1 1 5 0 2 14 21 16 22 34 13 9 1 1 1 7 0 2 0 0 1 0 2 2
Nucleus 205 391 229 184 192 212 240 276 183 189 79 102 128 96 65 261 228 134 203 384 232 87 59 56
Nuclear Periphery 0 0 0 0 2 0 3 6 2 2 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 6 3 8 15 20 43 42 56 67 80 37 40 6 15 19 1 4 2 3 4 6 6 20 25
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0
SpindlePole 2 0 0 0 0 0 0 0 0 0 0 0 3 2 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 3 2 3 3 4 1 7 1 3 0 0 2 0 2 9 11 4 7 7 8 18 28 36
Unique Cell Count 217 400 250 201 215 250 290 342 239 260 103 127 142 120 85 317 257 154 220 403 256 118 118 128
Labelled Cell Count 221 404 257 209 228 282 329 376 288 325 140 160 152 133 99 328 265 157 220 403 256 118 118 128


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.9 22.7 19.4 17.5 13.7 11.9 10.1 10.4 9.2 8.4 8.7 8.6 19.0 17.4 16.7 22.7 29.2 29.7 17.0 18.3 19.3
Std Deviation (1e-4) 5.7 6.2 7.8 7.6 5.1 5.0 4.3 3.8 3.5 3.1 3.0 3.0 7.4 7.4 6.8 9.7 11.6 12.7 4.8 5.6 5.0
Intensity Change (Log2) – – – -0.14 -0.5 -0.7 -0.94 -0.9 -1.07 -1.21 -1.15 -1.18 -0.03 -0.16 -0.21 0.23 0.59 0.61 -0.19 -0.08 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -0.4 -1.0 0.8 -1.4 -1.4 -1.6 1.3 -0.2 0.8 3.1 3.1 3.4 1.4 1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.1 2.8 1.7 3.5 4.7 4.1 0 0 0 0 0.2 0 0
Nucleus 0 -0.8 -2.4 -3.0 -3.7 -4.6 -5.6 -3.8 -3.2 -0.5 -3.2 -3.7 -3.2 -1.1 -1.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.0 2.8 5.2 4.5 5.1 7.6 8.2 8.4 7.8 0.5 3.5 5.6 0 -1.2 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 2.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.0007 0 0 0.0003 0.0468 0.0002 0.0038 0 0.0149 0 0 0
Bud – – – – – – 0.0001 0 0 0.0001 0.0008 0 0.0003 0 0.0002 0 0 0.0001
Bud Neck – – – – – – 0.0001 0 0 0 0.0005 0.0002 0.0002 0 0.0008 0 0 0.0001
Bud Periphery – – – – – – 0 0 0 0.0002 0.0015 0.0001 0.0004 0 0.0002 0 0 0.0001
Bud Site – – – – – – 0.0003 0 0 0.0004 0.0048 0 0.0001 0 0.0036 0 0 0
Cell Periphery – – – – – – 0 0 0 0 0.0002 0.0001 0.0001 0 0.0046 0 0 0.0002
Cytoplasm – – – – – – 0.0623 0 0 0.0004 0.0003 0.0007 0.009 0 0.0156 0 0 0.0001
Cytoplasmic Foci – – – – – – 0.0065 0 0 0.029 0.007 0.003 0.0004 0 0.0202 0 0 0.001
Eisosomes – – – – – – 0 0 0 0 0.0005 0 0 0 0.001 0 0 0
Endoplasmic Reticulum – – – – – – 0.0066 0 0 0.0002 0.0009 0.0002 0.0181 0 0.0016 0 0 0
Endosome – – – – – – 0.0638 0 0 0.0004 0.0127 0.0006 0.0192 0 0.0057 0 0 0.0002
Golgi – – – – – – 0.0013 0 0 0.0004 0.0222 0.0001 0.0007 0 0.0073 0 0 0
Lipid Particles – – – – – – 0.0019 0 0 0.0083 0.0266 0.0003 0.0003 0 0.0127 0 0 0.002
Mitochondria – – – – – – 0.001 0.0002 0.0002 0.0084 0.0241 0.0008 0.002 0.0002 0.0016 0.0005 0.0002 0.0011
None – – – – – – 0.0075 0 0 0.0017 0.0005 0.0003 0.0011 0 0.0014 0 0 0.0002
Nuclear Periphery – – – – – – 0.0295 0.0008 0.001 0.0669 0.004 0.0129 0.1582 0.0004 0.0115 0.0005 0 0.0005
Nucleolus – – – – – – 0.0052 0.0058 0.0054 0.0214 0.1624 0.0507 0.0045 0.0107 0.0103 0.0149 0.0216 0.0549
Nucleus – – – – – – 0.7997 0.9931 0.9932 0.8131 0.6674 0.915 0.7686 0.9885 0.8745 0.9839 0.978 0.9353
Peroxisomes – – – – – – 0.0002 0 0 0.0012 0.0114 0 0.0001 0 0.0025 0 0 0.0001
Punctate Nuclear – – – – – – 0.0065 0 0 0.0417 0.0033 0.0139 0.0012 0 0.006 0 0 0.0002
Vacuole – – – – – – 0.0053 0 0 0.0002 0.0007 0.0003 0.0054 0 0.0015 0 0 0.0014
Vacuole Periphery – – – – – – 0.0016 0.0001 0.0001 0.0057 0.0014 0.0006 0.0065 0 0.0023 0.0001 0 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 57.0261 77.2209 53.2722 44.5332 56.437 46.5399 44.9863 35.406 34.5994 48.0422
Translational Efficiency 1.5944 1.1711 1.2993 1.4335 1.3675 1.2881 1.4236 1.3496 1.252 1.2564

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1286 1283 820 1019 124 1945 181 1383 1410 3228 1001 2402

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1193.09 1394.98 1630.69 1755.44 1727.68 1750.54 1790.09 1872.30 1240.10 1609.22 1659.51 1822.72
Standard Deviation 222.80 436.39 319.97 346.29 333.46 412.04 278.98 395.60 279.24 456.36 318.91 379.89
Intensity Change Log 2 — 0.225542 0.450780 0.557130 — 0.018964 0.051196 0.115976 — 0.106950 0.227977 0.312722

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000430 0.000496 0.000617 0.000629 0.000448 0.000471 0.000477 0.000592 0.000431 0.000481 0.000592 0.000608
Bud Neck 0.001512 0.001177 0.006453 0.010573 0.000746 0.002009 0.004050 0.012013 0.001445 0.001678 0.006019 0.011402
Bud Site 0.000500 0.000907 0.000594 0.001339 0.000113 0.000538 0.000538 0.001695 0.000466 0.000685 0.000584 0.001544
Cell Periphery 0.000110 0.000424 0.000271 0.000116 0.000011 0.000363 0.000036 0.000206 0.000101 0.000387 0.000229 0.000168
Cytoplasm 0.000231 0.000691 0.000087 0.000145 0.000012 0.000145 0.000060 0.000525 0.000211 0.000362 0.000082 0.000364
Cytoplasmic Foci 0.000086 0.000342 0.000074 0.001348 0.000033 0.000474 0.001269 0.000898 0.000081 0.000421 0.000290 0.001089
Eisosomes 0.000170 0.000245 0.000179 0.000064 0.000173 0.000547 0.000121 0.000076 0.000170 0.000427 0.000168 0.000071
Endoplasmic Reticulum 0.001607 0.000636 0.000829 0.000777 0.000630 0.001323 0.000563 0.000622 0.001521 0.001050 0.000781 0.000688
Endosome 0.000033 0.000075 0.000033 0.000998 0.000004 0.000095 0.001067 0.000343 0.000031 0.000087 0.000220 0.000621
Golgi 0.000053 0.000248 0.000030 0.000521 0.000011 0.000070 0.002708 0.000969 0.000050 0.000141 0.000514 0.000779
Lipid Particles 0.000243 0.000188 0.000010 0.000049 0.000004 0.000025 0.000007 0.000230 0.000222 0.000090 0.000010 0.000153
Mitochondria 0.000693 0.000826 0.001423 0.002632 0.000663 0.000744 0.000968 0.002376 0.000690 0.000777 0.001341 0.002484
Mitotic Spindle 0.000422 0.000089 0.001243 0.000109 0.000001 0.000206 0.000013 0.003537 0.000385 0.000160 0.001021 0.002083
None 0.000841 0.004970 0.001079 0.001126 0.000353 0.001072 0.000245 0.000946 0.000798 0.002622 0.000928 0.001023
Nuclear Periphery 0.000030 0.000033 0.000097 0.000253 0.000006 0.000052 0.000026 0.000455 0.000028 0.000045 0.000084 0.000369
Nuclear Periphery Foci 0.000059 0.000132 0.000019 0.000269 0.000011 0.000148 0.000002 0.000228 0.000055 0.000141 0.000016 0.000246
Nucleolus 0.011616 0.008205 0.010454 0.010797 0.010893 0.006284 0.004593 0.010670 0.011552 0.007047 0.009394 0.010724
Nucleus 0.979526* 0.977685* 0.974279* 0.966003* 0.983040* 0.981837* 0.981513* 0.961538* 0.979835* 0.980187* 0.975587* 0.963432*
Peroxisomes 0.000602 0.000633 0.001441 0.000598 0.002598 0.000500 0.000939 0.000501 0.000778 0.000553 0.001351 0.000542
Vacuole 0.000930 0.001585 0.000655 0.001188 0.000210 0.002611 0.000427 0.001004 0.000867 0.002203 0.000614 0.001082
Vacuole Periphery 0.000305 0.000412 0.000132 0.000465 0.000043 0.000486 0.000379 0.000574 0.000282 0.000456 0.000177 0.000527

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.42 -4.03 -3.83 -3.00 -0.32 -0.70 -0.96 -2.96 -2.55 -2.32 -1.26 -3.94 -4.11 -3.75 -0.69
Bud Neck 2.01 -15.30 -9.34 -9.72 -4.51 -6.76 -8.71 -9.83 -8.80 -7.00 -1.38 -16.30 -12.71 -12.49 -7.27
Bud Site -0.55 -0.35 -1.41 -0.59 -1.37 -2.36 -1.71 -4.29 -2.95 -2.91 -0.60 -0.46 -2.98 -2.31 -3.18
Cell Periphery -3.04 -1.07 -0.06 2.94 1.04 -3.95 -1.96 -2.92 1.36 -2.53 -4.18 -1.03 -1.57 2.76 0.43
Cytoplasm 0.13 0.97 0.93 1.69 -0.68 -2.60 -1.35 -2.39 -1.68 -2.12 0.55 0.97 0.55 0.02 -2.03
Cytoplasmic Foci -1.31 0.06 -1.55 -1.23 -1.55 -1.83 -0.99 -2.11 -0.80 0.24 -1.94 -0.90 -2.35 -1.45 -1.72
Eisosomes -2.59 -0.28 7.93 6.80 4.38 -6.99 2.36 4.57 9.39 3.99 -7.59 0.11 7.98 11.09 4.50
Endoplasmic Reticulum 7.07 5.14 5.19 -1.18 0.51 -4.81 0.71 -0.02 5.16 -0.97 3.40 5.50 6.59 3.83 1.19
Endosome -0.98 -0.12 -1.50 -1.43 -1.50 -2.28 -1.00 -3.52 -2.36 0.65 -1.77 -0.99 -2.18 -1.95 -1.22
Golgi -0.90 0.68 -1.22 -0.56 -1.30 -1.54 -1.00 -1.31 -1.22 0.62 -0.96 -0.94 -1.59 -1.35 -0.39
Lipid Particles 0.26 1.07 0.89 0.74 -1.35 -1.70 -2.03 -1.76 -1.58 -1.73 0.67 1.07 0.41 -0.58 -1.91
Mitochondria -1.15 -5.02 -5.89 -5.70 -3.51 -0.90 -4.27 -4.80 -4.44 -3.88 -0.75 -5.22 -6.92 -6.78 -4.29
Mitotic Spindle 0.98 -1.17 0.92 -0.28 1.64 -1.50 -1.66 -2.85 -2.66 -2.84 0.73 -1.11 -2.08 -2.66 -0.68
None -3.76 -0.34 -0.48 3.20 -0.07 -2.10 1.68 -3.19 0.36 -3.68 -3.72 -0.21 -0.79 2.99 -0.19
Nuclear Periphery -0.31 -1.63 -3.01 -2.97 -1.70 -3.85 -3.23 -4.02 -3.60 -3.78 -1.63 -1.68 -4.78 -4.54 -3.41
Nuclear Periphery Foci -0.85 0.92 -1.06 -0.61 -1.27 -1.72 0.95 -2.91 -0.82 -3.03 -1.27 1.00 -1.94 -0.97 -2.45
Nucleolus 2.49 0.12 -0.32 -2.09 -0.41 2.49 3.32 -0.53 -3.62 -4.51 3.89 0.94 -0.39 -4.13 -1.29
Nucleus 0.40 2.26 3.99 3.64 2.16 0.58 0.26 6.12 6.10 3.62 -0.44 1.95 6.22 7.11 4.15
Peroxisomes -0.20 -5.22 -0.44 -0.16 5.06 5.24 4.13 5.18 -0.26 2.42 1.70 -4.10 1.52 -0.34 5.81
Vacuole -1.65 -0.23 -0.65 0.77 -0.17 -6.16 -2.05 -2.87 3.13 -2.01 -4.15 -0.17 -0.86 3.03 -0.29
Vacuole Periphery -0.58 1.49 -0.82 -0.27 -2.26 -4.80 -1.02 -3.11 -0.58 -0.67 -1.30 0.92 -1.60 -0.61 -2.74
Standard name
Human Ortholog
Description tRNA:pseudouridine synthase; introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; also acts on U2 snRNA; also pseudouridylates some mRNAs, and pseudouridylation level varies with growth phase; nuclear protein that appears to be involved in tRNA export; PUS1 has a paralog, PUS2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Pus1

Pus1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pus1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available