Standard name
Human Ortholog
Description Nucleolar protein required for 60S ribosome subunit biogenesis; constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0 0 0 0 0 0 0.08 0.07 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0.09 0.18 0.12 0.14 0.14 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.11 0 0.06 0.06 0.08 0.12 0.12 0.11 0.15 0.07 0.13 0.08 0 0 0 0.08 0.08 0.07
Nucleus 0.46 0.57 0.5 0 0.08 0.06 0.08 0.11 0.12 0.12 0.12 0.13 0.08 0.05 0.18 0.14 0.15 0.23 0.35 0.22 0.08 0.06 0.07
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.53 0.49 0.47 0.95 0.86 0.73 0.84 0.82 0.83 0.81 0.77 0.89 0.84 0.87 0.8 0.84 0.84 0.64 0.57 0.65 0.68 0.66 0.64
Peroxisomes 0 0 0 0.06 0.06 0 0.11 0 0.07 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.07 0.07 0.13 0.17 0.07 0.13 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.05 0.05 0 0 0.1 0 0 0 0 0 0 0 0 0.05 0 0.05 0.06 0.07 0.07 0.11 0.12 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 3
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 1 6 3 11 20 12 9 6 4 5 2 21 19 2 1 5 0 0 0 1 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 2 0 0 0 1 0 0 0
Endosome 0 0 0 9 23 37 108 64 69 37 15 2 3 1 0 0 0 3 0 0 2 4 4
Golgi 0 0 0 0 4 8 0 0 1 0 0 0 0 0 1 0 0 0 0 1 1 0 2
Mitochondria 5 4 2 16 14 43 9 32 28 21 19 21 29 43 23 34 23 0 0 0 12 23 24
Nucleus 102 126 101 10 33 23 51 61 61 31 20 23 21 14 59 39 43 59 97 54 12 18 26
Nuclear Periphery 0 0 3 0 0 4 4 7 4 6 0 0 0 0 0 2 0 7 1 10 2 3 5
Nucleolus 118 109 95 252 364 291 512 444 422 212 126 159 223 244 257 227 246 162 160 164 112 202 229
Peroxisomes 6 1 0 16 24 6 66 23 33 11 2 4 4 13 2 1 1 0 0 0 0 0 1
SpindlePole 0 0 0 18 29 50 105 40 66 21 14 0 4 5 0 0 1 2 1 0 0 8 4
Vac/Vac Membrane 11 12 10 0 12 41 15 5 7 5 6 1 2 1 17 9 14 15 18 16 18 36 52
Unique Cell Count 224 223 204 265 425 396 606 542 506 262 163 179 266 281 322 271 293 255 282 252 165 306 362
Labelled Cell Count 245 253 217 324 514 525 882 687 697 348 207 212 307 341 367 315 333 255 282 252 165 306 362


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 32.7 31.4 24.3 24.6 23.3 17.9 16.7 15.0 14.9 13.2 13.6 31.0 28.3 31.9 47.1 48.9 48.5 28.7 28.5 30.3
Std Deviation (1e-4) 9.3 9.4 6.7 9.8 8.7 11.9 4.9 4.3 5.2 3.9 3.8 13.1 12.0 17.2 19.4 16.0 16.6 7.4 9.5 10.3
Intensity Change (Log2) 0.02 -0.06 -0.44 -0.54 -0.69 -0.7 -0.88 -0.83 0.36 0.22 0.39 0.96 1.01 1.0 0.24 0.23 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -0.3 1.2 0 0 0 0 0 0 2.3 1.9 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.4 4.5 6.5 5.1 5.6 5.6 4.4 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.8 1.7 4.4 0 2.9 2.7 3.5 4.4 4.4 4.3 5.4 3.2 4.7 3.4
Nucleus -11.6 -12.0 -12.5 -13.0 -11.3 -10.8 -9.0 -7.5 -7.7 -10.2 -11.4 -7.6 -8.3 -8.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 11.9 10.3 6.5 10.8 9.6 10.0 7.8 6.0 8.7 8.6 9.5 7.9 8.6 8.8
Peroxisomes 3.6 3.5 0 4.9 3.0 3.7 0 0 0 0 3.1 0 0 0
SpindlePole 3.8 3.8 5.3 6.4 4.0 5.4 4.1 4.3 0 0 0 0 0 0
Vacuole 0 -1.3 2.3 -1.7 0 0 -1.8 -0.6 -2.5 -2.8 0 0.2 -0.9 -0.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.1456 25.0459 20.9899 18.9685 17.0904 21.0356 25.5295 28.6623 26.8735 24.829 19.6579 25.0908 29.5182 34.882 29.7996 27.4908 22.9511 30.8833
Actin 0.013 0 0.0059 0.0004 0 0.0002 0.0052 0 0.0014 0.0022 0.0088 0.0021 0.003 0 0.0206 0.0277 0.0842 0.0022
Bud 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0.0003 0 0 0.0002 0.0003 0.0001 0.0001 0 0.0003 0.0001 0.0002 0.0001
Bud Neck 0.0002 0 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0003 0.0002 0.0002 0.0001
Bud Periphery 0.0002 0.0001 0.0003 0.0002 0.0002 0.0003 0.0005 0.0001 0 0.0003 0.001 0.0003 0.0002 0.0001 0.0005 0.0003 0.0003 0.0003
Bud Site 0.0008 0.0001 0.0013 0.0002 0.0001 0.0001 0.0013 0.0001 0.0001 0.0005 0.0006 0.0001 0.0003 0.0001 0.0005 0.0003 0.0003 0.0002
Cell Periphery 0.0006 0.0004 0.0003 0.0008 0.0006 0.0012 0.0006 0.0006 0.0003 0.0003 0.0012 0.0008 0.0011 0.0005 0.0006 0.0002 0.0006 0.0007
Cytoplasm 0.0081 0 0.0102 0.0013 0 0.0019 0.0042 0.0002 0.0016 0.0015 0.0021 0.0045 0.0022 0 0.002 0.0001 0.0005 0.0006
Cytoplasmic Foci 0.0112 0 0.0055 0.0276 0.0026 0.0012 0.0087 0.0001 0.0019 0.0052 0.0015 0.0061 0.0264 0 0.0007 0.0033 0.022 0.0099
Eisosomes 0.0002 0 0.0001 0.0001 0 0.0001 0.0002 0 0 0.0001 0.0008 0.0001 0.0001 0 0.0002 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0043 0 0.0072 0.0002 0 0.0028 0.0011 0 0.0002 0.0014 0.0107 0.0015 0.0006 0 0.0149 0.0003 0.0007 0.0001
Endosome 0.0147 0 0.0114 0.0005 0 0.0031 0.0046 0.0004 0.0017 0.0207 0.0197 0.0061 0.0111 0 0.0112 0.0055 0.0065 0.0005
Golgi 0.0039 0 0.0028 0.0003 0 0.0002 0.001 0 0.0007 0.0059 0.0035 0.0026 0.003 0 0.0061 0.0176 0.0252 0.0017
Lipid Particles 0.0063 0.0001 0.0037 0.0087 0.002 0.0059 0.0038 0.0003 0.0022 0.0027 0.0125 0.0062 0.01 0.0001 0.0023 0.0085 0.0141 0.0112
Mitochondria 0.0157 0.0008 0.0166 0.0085 0.0395 0.0186 0.0188 0.0027 0.0015 0.0062 0.0472 0.0184 0.0034 0.0024 0.0139 0.0063 0.0243 0.0173
None 0.0027 0 0.0103 0.0052 0.0001 0.0004 0.003 0 0.0083 0.0005 0.0014 0.0019 0.0013 0 0.0002 0.0002 0.0009 0.0009
Nuclear Periphery 0.0092 0.0006 0.0101 0.0502 0.0001 0.0376 0.0042 0.0096 0.0016 0.0067 0.083 0.0356 0.0154 0.0002 0.0057 0.0026 0.0138 0.0233
Nucleolus 0.7963 0.7884 0.7818 0.8057 0.8527 0.6999 0.8036 0.8075 0.8453 0.7695 0.5976 0.7401 0.7691 0.8335 0.7878 0.7401 0.7027 0.7695
Nucleus 0.0945 0.2063 0.1245 0.0795 0.097 0.1847 0.1272 0.1582 0.1297 0.171 0.1521 0.1367 0.0959 0.1599 0.1211 0.1817 0.067 0.1319
Peroxisomes 0.0022 0 0.001 0.0013 0.0014 0.0001 0.0013 0 0.0012 0.0003 0.0016 0.0002 0.0254 0 0.0001 0.003 0.0014 0.0008
Punctate Nuclear 0.0055 0.0001 0.0038 0.0012 0.0002 0.0012 0.0043 0.0002 0.0006 0.0008 0.0015 0.0015 0.0181 0.0001 0.0002 0.0005 0.0009 0.0019
Vacuole 0.0015 0.0012 0.0011 0.0015 0.0011 0.0055 0.0016 0.0073 0.0005 0.0013 0.0052 0.0029 0.0016 0.0009 0.0031 0.0004 0.0021 0.0017
Vacuole Periphery 0.0085 0.0019 0.0016 0.0063 0.0022 0.0349 0.0043 0.0125 0.0011 0.0025 0.0476 0.032 0.0115 0.0017 0.0077 0.001 0.0318 0.0251

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 129.3368 86.7144 108.5836 85.8774 100.8765 62.6128 88.1335 80.7505 60.1699 92.2913
Translational Efficiency 1.8466 2.6585 1.5303 1.3158 1.621 2.7132 1.7712 1.6301 1.9062 1.9546

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1952 1135 3381 8 1227 2697 2422 71 3179 3832 5803 79

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1644.61 1920.55 2973.92 2558.86 1761.41 2125.58 3046.82 3781.95 1689.69 2064.85 3004.35 3658.09
Standard Deviation 479.27 614.64 792.77 1971.15 419.21 529.28 882.71 963.16 460.55 563.76 832.27 1167.62
Intensity Change Log 2 0.223774 0.854620 0.637756 0.271126 0.790574 1.102400 0.248456 0.821854 0.896580

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000981 0.001097 0.001574 0.000764 0.000778 0.000690 0.001832 0.001587 0.000902 0.000811 0.001682 0.001504
Bud Neck 0.001045 0.002252 0.001441 0.003262 0.000943 0.001529 0.003281 0.006720 0.001005 0.001743 0.002209 0.006370
Bud Site 0.000077 0.000292 0.000097 0.000252 0.000082 0.000175 0.000350 0.001094 0.000079 0.000210 0.000203 0.001008
Cell Periphery 0.000025 0.000062 0.000030 0.000110 0.000015 0.000025 0.000105 0.000080 0.000021 0.000036 0.000061 0.000083
Cytoplasm 0.000048 0.000216 0.000056 0.000061 0.000017 0.000150 0.000310 0.000399 0.000036 0.000170 0.000162 0.000365
Cytoplasmic Foci 0.000076 0.001583 0.000233 0.000477 0.000148 0.000568 0.001848 0.006671 0.000104 0.000869 0.000907 0.006044
Eisosomes 0.000093 0.000138 0.000141 0.000159 0.000121 0.000164 0.000166 0.000110 0.000104 0.000156 0.000151 0.000115
Endoplasmic Reticulum 0.000329 0.000237 0.000173 0.000029 0.000196 0.000171 0.000177 0.000098 0.000278 0.000191 0.000175 0.000091
Endosome 0.000013 0.000058 0.000009 0.000008 0.000010 0.000043 0.000019 0.000033 0.000012 0.000047 0.000013 0.000030
Golgi 0.000038 0.000194 0.000064 0.000113 0.000050 0.000064 0.000125 0.000611 0.000042 0.000103 0.000090 0.000560
Lipid Particles 0.000004 0.000157 0.000004 0.000008 0.000009 0.000031 0.000015 0.000053 0.000006 0.000068 0.000009 0.000048
Mitochondria 0.001691 0.002944 0.002207 0.002057 0.001558 0.001136 0.004496 0.011448 0.001640 0.001671 0.003163 0.010497
Mitotic Spindle 0.000068 0.000618 0.000349 0.000031 0.000685 0.000649 0.000394 0.020442 0.000306 0.000640 0.000368 0.018375
None 0.001098 0.001795 0.000406 0.377300* 0.000276 0.000372 0.001557 0.000902 0.000781 0.000793 0.000886 0.039018
Nuclear Periphery 0.002320 0.001703 0.008142 0.000182 0.004479 0.001132 0.006183 0.032410 0.003153 0.001301 0.007325 0.029146
Nuclear Periphery Foci 0.000375 0.000769 0.001205 0.000125 0.001404 0.000369 0.000904 0.008710 0.000772 0.000487 0.001080 0.007841
Nucleolus 0.456477* 0.537329* 0.510478* 0.464004* 0.625179* 0.504422* 0.514372* 0.641281* 0.521591* 0.514169* 0.512103* 0.623329*
Nucleus 0.527157* 0.425000* 0.453384* 0.133753* 0.349315* 0.478056* 0.430250* 0.188560* 0.458515* 0.462341* 0.443728* 0.183010*
Peroxisomes 0.004654 0.011311 0.009085 0.005860 0.010660 0.007721 0.010861 0.033500 0.006972 0.008784 0.009826 0.030701
Vacuole 0.000656 0.002054 0.000567 0.000967 0.000469 0.000359 0.002359 0.002901 0.000584 0.000861 0.001315 0.002705
Vacuole Periphery 0.002776 0.010190 0.010354 0.010478 0.003606 0.002174 0.020396 0.042392 0.003096 0.004548 0.014545 0.039160

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN 2.35 -20.20 -2.77 -3.09 1.08 3.06 -22.95 -2.24 -2.60 0.91
Bud Neck NaN NaN NaN NaN NaN -3.22 -11.29 -6.58 -5.99 -4.13 -6.95 -12.94 -6.57 -5.67 -5.19
Bud Site NaN NaN NaN NaN NaN -2.96 -8.33 -3.73 -3.31 -2.82 -4.69 -8.55 -3.77 -3.19 -3.31
Cell Periphery NaN NaN NaN NaN NaN -2.04 -5.70 -3.57 -3.00 0.92 -3.91 -5.82 -3.59 -2.72 -1.27
Cytoplasm NaN NaN NaN NaN NaN -1.62 -4.38 -1.39 -0.91 -0.78 -1.98 -4.39 -1.35 -0.89 -1.06
Cytoplasmic Foci NaN NaN NaN NaN NaN -2.70 -4.19 -1.89 -1.77 -1.47 -3.43 -4.65 -1.90 -1.69 -1.69
Eisosomes NaN NaN NaN NaN NaN -4.80 -5.25 0.49 2.85 2.98 -8.24 -8.93 -0.81 2.25 1.99
Endoplasmic Reticulum NaN NaN NaN NaN NaN 1.02 0.92 4.15 4.04 4.64 4.55 7.46 9.98 5.42 5.81
Endosome NaN NaN NaN NaN NaN -1.33 -3.98 -4.07 0.48 -2.52 -1.93 -1.01 -3.63 0.99 -3.26
Golgi NaN NaN NaN NaN NaN -1.27 -10.82 -4.80 -4.59 -4.15 -3.06 -13.30 -4.87 -4.15 -4.42
Lipid Particles NaN NaN NaN NaN NaN -1.35 -2.75 -3.93 -0.37 -3.31 -2.18 -2.99 -4.20 1.00 -3.87
Mitochondria NaN NaN NaN NaN NaN 2.21 -12.15 -2.94 -3.07 -2.02 -0.31 -12.88 -2.91 -2.90 -2.39
Mitotic Spindle NaN NaN NaN NaN NaN -0.11 0.42 -1.35 -1.34 -1.36 -1.39 -0.44 -1.37 -1.34 -1.36
None NaN NaN NaN NaN NaN -2.53 -4.37 -4.82 -3.86 1.94 -0.15 -0.65 -1.77 -1.77 -1.76
Nuclear Periphery NaN NaN NaN NaN NaN 3.67 -1.50 -2.07 -2.35 -1.94 4.15 -6.53 -2.15 -2.32 -1.78
Nuclear Periphery Foci NaN NaN NaN NaN NaN 3.35 1.78 -1.97 -2.27 -2.13 1.65 -2.34 -2.14 -2.22 -2.04
Nucleolus NaN NaN NaN NaN NaN 12.38* 11.10 0.00 -3.33 -3.05 1.04 1.38 -2.44 -2.67 -2.73
Nucleus NaN NaN NaN NaN NaN -12.97 -7.98 4.54 8.69 7.15 -0.47 2.13 9.08 9.24 8.64
Peroxisomes NaN NaN NaN NaN NaN 4.87 -0.55 -3.17 -3.54 -3.12 -4.90 -7.68 -3.58 -3.33 -3.18
Vacuole NaN NaN NaN NaN NaN 0.61 -7.88 -4.07 -4.34 -0.87 -2.61 -6.80 -3.99 -3.40 -2.60
Vacuole Periphery NaN NaN NaN NaN NaN 2.25 -13.75 -2.64 -2.75 -1.42 -3.05 -19.85 -2.73 -2.61 -1.81
Standard name
Human Ortholog
Description Nucleolar protein required for 60S ribosome subunit biogenesis; constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p
Localization
Cell Percentages nucleus (43%), nucleolus (9%), mixed (46%)
Cell Cycle Regulation Yes
nucleus - MA (3e-05)
Subcompartmental Group N/A

Nip7

Nip7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nip7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available