Standard name
Human Ortholog
Description Phosphatidylglycerol phospholipase C; regulates phosphatidylglycerol (PG) accumulation via a phospholipase C-type degradation mechanism; PG levels affect mitochondrial function; contains glycerophosphodiester phosphodiesterase motifs
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud 0 0 0 0 0 0 0 0.06 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.78 0.83 0.95 0.91 0.96 0.81 0.68 0.62 0.37 0.5 0.21 0.32 0.77 0.77 0.82 0.46 0.45 0.37 0.36 0.39 0.27 0.43 0.46 0.43
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.11 0.07 0 0 0 0 0 0
Endosome 0 0.08 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.12 0.24 0.4 0.41 0.13 0.12 0.08 0.08 0.07 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.07 0.05 0.05 0 0.17 0.4 0.48 0.69 0.61 0.88 0.73 0 0 0 0.05 0.07 0.08 0.29 0.28 0.46 0.28 0.26 0.21
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
SpindlePole 0 0 0 0.06 0 0 0 0 0 0 0 0 0.14 0.11 0.07 0.18 0.12 0.14 0.05 0.05 0 0.09 0.06 0.07
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.15 0.1 0.14 0.05 0.06 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 1 0 0 1 0 0 1 1 0 0 0 0 10 2 2 6 15 10
Bud 3 2 0 1 0 0 9 13 11 6 4 6 0 0 0 1 0 4 2 0 0 3 6 9
Bud Neck 2 0 0 2 3 4 4 5 0 2 0 2 3 7 5 2 1 1 2 4 0 2 3 6
Bud Site 0 0 0 0 1 0 1 0 2 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 1 0 0 0 0 1 1 0 2 1 0 1 1 0 0 2 3
Cytoplasm 205 121 19 147 193 135 171 139 73 106 31 42 193 228 304 110 89 115 119 71 21 104 141 163
Endoplasmic Reticulum 6 2 0 1 2 6 0 0 0 0 2 0 4 1 0 24 21 22 8 3 1 2 3 6
Endosome 1 11 0 1 1 2 4 1 0 1 0 0 17 30 44 58 78 125 44 21 6 19 20 32
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 1 2 0 3 1 0 1 1 0 0 0 1
Mitochondria 50 10 1 8 2 29 101 108 134 128 132 96 0 1 1 12 13 24 95 50 36 67 80 78
Nucleus 0 0 0 0 2 0 3 1 2 3 1 6 1 0 0 3 2 0 0 0 0 0 0 2
Nuclear Periphery 2 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0 1 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 1 0 0 1 1 1 0 1 6 26 3 0 1 2 3 2
SpindlePole 9 4 0 10 3 3 6 6 5 4 2 4 34 34 27 43 24 43 14 9 2 21 19 26
Vac/Vac Membrane 2 4 0 0 0 3 3 6 2 5 1 9 3 7 6 37 20 43 15 10 2 5 6 22
Unique Cell Count 262 146 20 162 200 167 252 226 195 211 150 132 251 297 372 241 197 308 332 181 80 242 311 379
Labelled Cell Count 281 154 20 172 208 182 303 281 230 257 173 168 259 313 387 296 257 403 332 181 80 242 311 379


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.3 4.4 4.6 4.6 3.6 3.5 3.9 3.1 3.5 3.1 3.5 5.4 5.4 5.4 6.6 6.1 5.7 5.2 5.3 5.6
Std Deviation (1e-4) 0.5 1.0 1.5 1.4 1.5 1.1 1.2 1.2 0.6 1.2 0.8 1.0 1.4 1.7 1.5 1.9 2.1 2.0 1.7 2.1 2.0
Intensity Change (Log2) – – – 0.05 0.05 -0.31 -0.35 -0.19 -0.53 -0.35 -0.5 -0.33 0.29 0.3 0.28 0.57 0.46 0.35 0.23 0.27 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6914 -0.7493 -0.6116 -0.1382 -1.5097 -0.6261 1.1269 0.0806 0.1521 -0.133 -0.2119 -0.2865 -0.6544 -0.3968 -0.2222 -0.6104 -0.2626 -0.3034
Actin 0.0353 0.0027 0.0069 0.0403 0.0002 0.0011 0.0213 0.0018 0.0134 0.0126 0.0185 0.0011 0.0002 0.0021 0.0009 0.0013 0.0002 0.0004
Bud 0.0008 0.0005 0.0005 0.0015 0.0001 0.001 0.0015 0.0036 0.001 0.0002 0.0021 0.0002 0.0036 0.0057 0.0104 0.0032 0.0001 0.0054
Bud Neck 0.0129 0.0009 0.0123 0.0177 0.0004 0.0045 0.0103 0.0006 0.0039 0.009 0.0026 0.0059 0.0005 0.0019 0.0102 0.0005 0.0002 0.0011
Bud Periphery 0.0017 0.0007 0.001 0.0025 0.0001 0.0012 0.0037 0.001 0.0012 0.0002 0.0014 0.0009 0.0007 0.004 0.0036 0.002 0.0001 0.0013
Bud Site 0.0059 0.0096 0.0023 0.0162 0.0002 0.0031 0.0137 0.0085 0.0076 0.001 0.0115 0.0015 0.0064 0.0197 0.0052 0.0021 0.0002 0.0009
Cell Periphery 0.0007 0.0004 0.0003 0.001 0 0.0005 0.0014 0.0005 0.0007 0.0001 0.0003 0.001 0.0003 0.0011 0.0005 0.0002 0.0001 0.0003
Cytoplasm 0.0294 0.0376 0.0343 0.0191 0.0149 0.0273 0.0345 0.0385 0.0304 0.0115 0.0689 0.0424 0.0131 0.0367 0.0161 0.0275 0.0156 0.0262
Cytoplasmic Foci 0.1758 0.1137 0.1826 0.1722 0.2137 0.1543 0.1752 0.1653 0.1637 0.2293 0.2149 0.2102 0.1619 0.1487 0.1639 0.1926 0.1896 0.1837
Eisosomes 0.0008 0.0006 0.0003 0.0012 0 0.0003 0.0004 0.0002 0.0055 0.0001 0.0003 0.0001 0.0001 0.0004 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0035 0.0041 0.0045 0.0041 0.001 0.003 0.0074 0.0049 0.0058 0.0009 0.004 0.0033 0.0013 0.0045 0.0034 0.0023 0.0034 0.0031
Endosome 0.1575 0.1297 0.1215 0.0723 0.1034 0.1078 0.1365 0.1575 0.1349 0.0964 0.1118 0.1418 0.2183 0.1819 0.2016 0.234 0.2057 0.1865
Golgi 0.0134 0.0109 0.0114 0.0158 0.0117 0.0085 0.0185 0.0134 0.0118 0.0199 0.0196 0.0141 0.0281 0.0249 0.0348 0.0151 0.0241 0.0172
Lipid Particles 0.267 0.1492 0.2234 0.1221 0.4459 0.2521 0.2655 0.23 0.2354 0.2736 0.2217 0.2955 0.3943 0.1896 0.2969 0.211 0.2699 0.3589
Mitochondria 0.0095 0.0043 0.0085 0.0071 0.0033 0.009 0.0192 0.0071 0.0053 0.0071 0.0037 0.0215 0.0072 0.0049 0.0111 0.0033 0.0049 0.0044
None 0.1541 0.431 0.2459 0.4084 0.0098 0.2364 0.127 0.266 0.2462 0.1472 0.2105 0.114 0.0713 0.2239 0.0979 0.0838 0.1649 0.1031
Nuclear Periphery 0.0059 0.0105 0.0135 0.0083 0.0014 0.0101 0.0239 0.0072 0.0115 0.0076 0.0091 0.0077 0.0026 0.0086 0.0055 0.0078 0.0071 0.0043
Nucleolus 0.0239 0.0079 0.0056 0.0063 0.0006 0.0134 0.0046 0.0066 0.0045 0.0046 0.0025 0.0065 0.008 0.0163 0.0033 0.0041 0.002 0.0025
Nucleus 0.0093 0.0088 0.0083 0.0085 0.0005 0.0228 0.0309 0.0055 0.0099 0.0011 0.0147 0.005 0.0043 0.0112 0.0085 0.0082 0.0037 0.0035
Peroxisomes 0.0507 0.0277 0.0618 0.0296 0.1398 0.0663 0.0576 0.0278 0.0408 0.1235 0.0495 0.06 0.0455 0.05 0.0877 0.0866 0.0638 0.0553
Punctate Nuclear 0.0207 0.0336 0.0429 0.0358 0.0486 0.0684 0.0346 0.035 0.0544 0.0422 0.0268 0.0489 0.0207 0.0416 0.0272 0.1068 0.0135 0.0297
Vacuole 0.0192 0.0131 0.0097 0.0084 0.0035 0.0072 0.0098 0.0158 0.0098 0.01 0.0047 0.0144 0.0095 0.0195 0.0086 0.0061 0.0261 0.0097
Vacuole Periphery 0.0022 0.0025 0.0025 0.0018 0.0009 0.0019 0.0023 0.0031 0.0025 0.0019 0.0011 0.0039 0.0018 0.0027 0.0026 0.0015 0.0047 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.1358 16.3375 21.9732 26.2708 23.6954 26.257 25.8107 29.6148 42.7387 28.9062
Translational Efficiency 3.463 2.946 2.2021 2.0687 1.9657 2.1546 2.2954 1.8413 1.6065 1.7818

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1817 643 2040 1606 1855 2089 888 1116 3672 2732 2928 2722

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 663.26 705.49 771.19 750.66 647.60 710.02 798.13 799.16 655.35 708.95 779.36 770.54
Standard Deviation 81.65 89.47 86.41 122.72 74.47 83.29 91.30 112.64 78.50 84.81 88.79 121.07
Intensity Change Log 2 — 0.089051 0.217512 0.178585 — 0.132757 0.301521 0.303381 — 0.110808 0.259626 0.241587

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000154 0.001363 0.000146 0.003305 0.000230 0.000419 0.000130 0.002174 0.000193 0.000642 0.000141 0.002841
Bud Neck 0.015538 0.018820 0.009398 0.020123 0.011223 0.025199 0.007776 0.017140 0.013358 0.023697 0.008906 0.018900
Bud Site 0.002038 0.009677 0.008212 0.044794 0.003081 0.014322 0.004791 0.020579 0.002565 0.013229 0.007174 0.034866
Cell Periphery 0.000136 0.000178 0.000194 0.000223 0.000133 0.000132 0.000215 0.000171 0.000134 0.000143 0.000200 0.000202
Cytoplasm 0.307426* 0.121577* 0.414500* 0.319255* 0.350156* 0.196855* 0.388372* 0.369982* 0.329012* 0.179138* 0.406576* 0.340052*
Cytoplasmic Foci 0.374765* 0.436416* 0.042940 0.155870 0.322229* 0.339902* 0.077152 0.114509 0.348225* 0.362617* 0.053316 0.138912
Eisosomes 0.000313 0.000309 0.000067 0.000146 0.000215 0.000159 0.000116 0.000111 0.000264 0.000194 0.000082 0.000132
Endoplasmic Reticulum 0.003682 0.002443 0.001967 0.004823 0.001791 0.002735 0.001554 0.002041 0.002726 0.002666 0.001842 0.003682
Endosome 0.027651 0.095815 0.005823 0.041539 0.026125 0.088549 0.009342 0.032460 0.026880 0.090259 0.006890 0.037817
Golgi 0.008842 0.031513 0.000229 0.004568 0.005474 0.014863 0.000408 0.005715 0.007141 0.018781 0.000283 0.005038
Lipid Particles 0.064073 0.063912 0.005129 0.026741 0.067082 0.076433 0.006409 0.011335 0.065593 0.073486 0.005517 0.020425
Mitochondria 0.014485 0.045162 0.001584 0.004797 0.007340 0.030102 0.000857 0.003947 0.010876 0.033647 0.001364 0.004449
Mitotic Spindle 0.001123 0.011423 0.008068 0.058978 0.004391 0.016748 0.003666 0.055461 0.002774 0.015495 0.006733 0.057536
None 0.004336 0.001638 0.007202 0.004454 0.005720 0.003471 0.007345 0.005331 0.005035 0.003039 0.007245 0.004813
Nuclear Periphery 0.001295 0.000748 0.004484 0.003653 0.001620 0.002008 0.002221 0.002564 0.001459 0.001711 0.003798 0.003207
Nuclear Periphery Foci 0.000757 0.001205 0.001923 0.011611 0.001695 0.001711 0.002425 0.004743 0.001231 0.001592 0.002075 0.008795
Nucleolus 0.002646 0.003406 0.002921 0.004022 0.003561 0.006100 0.004369 0.006644 0.003108 0.005466 0.003360 0.005097
Nucleus 0.111727 0.037752 0.445465* 0.119002 0.146833* 0.073297 0.426409* 0.169295* 0.129462 0.064931 0.439686* 0.139622*
Peroxisomes 0.017470 0.052878 0.001898 0.023509 0.012455 0.024024 0.004069 0.014254 0.014937 0.030815 0.002557 0.019715
Vacuole 0.039779 0.057270 0.037542 0.145787 0.027541 0.077657 0.052070 0.157338 0.033597 0.072858 0.041948 0.150523
Vacuole Periphery 0.001763 0.006495 0.000307 0.002800 0.001104 0.005314 0.000305 0.004208 0.001430 0.005592 0.000306 0.003377

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.93 -2.76 -6.28 -2.57 -6.12 -2.06 0.90 -2.63 -2.20 -2.76 -4.65 -0.46 -6.54 -4.84 -6.51
Bud Neck -1.56 4.00 -3.84 -1.21 -7.50 -9.29 1.99 -2.75 4.84 -4.00 -8.54 3.78 -5.11 3.33 -8.07
Bud Site -5.52 -5.16 -11.75 -8.03 -9.14 -6.44 -1.31 -6.81 -0.80 -5.53 -8.19 -4.64 -13.33 -6.35 -10.35
Cell Periphery -1.79 -3.00 -4.85 -0.56 -1.27 0.17 -3.60 -0.12 -0.22 3.03 -0.83 -4.69 -3.81 -2.30 1.23
Cytoplasm 20.11 -8.66 0.97 -18.00 8.99 19.63 -2.07 1.31 -13.75 2.89 25.22 -7.53 2.16 -19.77 8.70
Cytoplasmic Foci -5.87 56.10 34.50 30.03 -19.53 -2.23 34.31 31.99 35.82 -3.14 -2.39 66.90 45.91 44.41 -18.00
Eisosomes 0.17 16.71 11.73 6.67 -10.47 5.28 7.34 10.20 6.53 1.28 6.24 19.38 15.05 8.18 -7.54
Endoplasmic Reticulum 1.63 1.11 -2.82 -5.72 -7.55 -3.00 -1.96 -3.75 0.38 -1.36 0.18 -0.12 -4.48 -5.56 -6.95
Endosome -11.71 13.36 -2.63 10.38 -13.88 -18.19 6.98 -0.29 17.01 -6.48 -21.97 15.29 -2.37 19.65 -15.42
Golgi -9.05 14.69 7.78 11.48 -6.22 -8.25 10.40 1.96 7.90 -2.72 -11.05 18.03 6.34 14.18 -5.65
Lipid Particles 0.05 21.46 13.45 8.54 -10.95 -2.10 19.59 18.22 21.16 -2.71 -2.37 29.07 21.54 20.61 -11.17
Mitochondria -6.64 10.76 8.43 9.26 -5.85 -12.06 9.03 5.58 15.25 -4.39 -12.35 13.58 9.92 17.53 -6.97
Mitotic Spindle -4.64 -6.76 -13.64 -9.66 -10.97 -5.69 0.34 -8.29 -5.54 -8.48 -8.00 -4.61 -15.56 -10.52 -13.57
None 3.63 -2.31 0.70 -2.52 2.88 2.10 -0.70 1.15 -0.63 1.63 2.76 -1.90 1.44 -1.07 3.23
Nuclear Periphery 3.22 -11.47 -10.49 -12.24 2.64 -1.83 -4.62 -6.01 -5.03 -2.20 -1.74 -11.98 -11.81 -10.61 2.21
Nuclear Periphery Foci -2.42 -5.75 -9.42 -9.00 -6.70 -0.92 -3.63 -4.43 -4.14 -1.89 -2.89 -6.19 -10.25 -9.51 -6.76
Nucleolus -1.00 -3.04 -4.32 -2.42 -1.14 -4.71 -1.94 -3.95 -1.14 -1.60 -5.53 -3.25 -5.64 -0.94 -2.17
Nucleus 14.88 -46.76 -4.31 -16.71 40.17 15.21 -26.08 -3.25 -14.15 21.44 18.55 -52.23 -4.58 -19.40 44.62
Peroxisomes -8.91 14.76 -0.88 8.20 -11.28 -8.10 6.33 1.32 7.71 -3.65 -11.06 16.23 -0.43 9.31 -11.61
Vacuole -4.03 -5.47 -27.76 -20.62 -23.43 -16.56 -10.66 -26.30 -19.07 -18.17 -15.38 -11.64 -38.52 -27.52 -29.92
Vacuole Periphery -5.88 10.29 -0.18 4.85 -2.97 -9.85 5.20 -2.19 3.98 -3.58 -11.13 10.98 -1.63 6.48 -4.55
Standard name
Human Ortholog
Description Phosphatidylglycerol phospholipase C; regulates phosphatidylglycerol (PG) accumulation via a phospholipase C-type degradation mechanism; PG levels affect mitochondrial function; contains glycerophosphodiester phosphodiesterase motifs
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Pgc1

Pgc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pgc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available