ORF
Description Putative protein of unknown function; predicted to be palmitoylated

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.12 0.09 0.05 0.06 0.05 0.07 0 0.05
Cytoplasm 0.94 0.98 0.99 0.99 0.98 0.95 0.96 0.86 0.86 0.77 0.81 0.99 0.99 0.99 0.96 0.93 0.93 0.65 0.73 0.68 0.72 0.75 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0 0.19 0.17 0.29 0.21 0 0 0 0 0 0 0.08 0.06 0.13 0.07 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 8 17 11 10 10 9
Bud 1 0 0 0 0 1 1 4 3 7 5 1 0 0 0 0 0 1 6 3 3 8 14
Bud Neck 0 3 20 10 18 8 15 11 18 11 11 1 1 1 5 3 2 4 7 3 5 10 7
Bud Site 0 0 0 0 0 1 0 0 0 2 3 0 0 0 0 0 0
Cell Periphery 1 7 10 4 3 1 8 2 0 4 2 11 11 5 14 24 16 7 21 15 21 12 16
Cytoplasm 117 376 334 275 350 303 374 319 334 322 307 349 387 366 233 184 171 95 272 200 208 258 246
Endoplasmic Reticulum 1 1 2 0 2 0 0 0 0 1 1 0 1 2 15 10 15 4 3 3 3 3 3
Endosome 0 1 1 0 0 0 2 0 1 2 0 0 1 1 2 1 1 3 2 2 3 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3 2 2 3 3 1 0 0 0 2 2
Mitochondria 5 5 0 0 0 37 12 70 67 120 80 0 0 0 4 3 3 12 21 38 20 19 9
Nucleus 1 0 0 1 0 4 3 3 1 2 1 0 1 1 2 1 1 0 0 0 1 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 3 5 3 14 11 15 14 18 0 1 0 1 2 1 0 1 0 0 3 3
Vac/Vac Membrane 0 0 0 1 1 0 1 4 5 1 2 0 0 0 1 2 4 1 4 0 2 3 8
Unique Cell Count 124 382 339 278 357 318 391 373 390 420 377 351 391 370 243 198 184 148 372 292 291 346 337
Labelled Cell Count 126 393 367 294 379 358 430 425 444 487 431 363 406 378 280 234 218 148 372 292 291 346 337


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.9 4.6 5.0 5.0 3.4 4.2 3.6 3.8 3.8 3.6 4.9 5.2 5.3 5.6 6.2 6.3 4.0 4.4 4.5
Std Deviation (1e-4) 1.4 0.6 1.1 5.7 1.7 1.0 1.7 1.4 1.3 1.3 0.8 1.2 1.7 2.1 1.6 1.9 2.8 1.5 1.9 1.9
Intensity Change (Log2) 0.13 0.12 -0.43 -0.13 -0.35 -0.27 -0.29 -0.35 0.09 0.17 0.19 0.28 0.43 0.44 -0.2 -0.05 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypl199c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available