Standard name
Human Ortholog
Description Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 – – – 0.08 0.07 0.08 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.08 0 0.05 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.12 0.11 0.16 0.15 0.08 0.07
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.86 0.95 0.93 0.9 0.95 0.95 0.95 0.96 0.92 0.94 0.91 0.88 0.87 0.89 0.92 – – – 0.2 0.46 0.34 0.42 0.62 0.63
Endoplasmic Reticulum 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.07 0.08 0.08 0.1 0 0 0 – – – 0.12 0.05 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0.07 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.23 0.22 0.15 0.22 0.14 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 7 12 6 1 7 3 2 1 1 3 4 11 11 – – – 6 17 27 4 9 8
Bud 0 7 3 10 7 6 9 7 19 5 16 18 2 9 5 – – – 6 5 16 5 11 9
Bud Neck 0 3 14 7 10 5 15 18 6 8 11 6 2 9 6 – – – 10 24 55 24 18 16
Bud Site 0 0 4 5 0 1 3 2 4 2 6 3 0 2 0 – – – – – – – – –
Cell Periphery 0 2 7 8 5 4 3 4 5 2 1 7 0 3 3 – – – 1 2 10 3 4 3
Cytoplasm 25 205 313 254 470 398 540 595 417 433 347 426 69 183 226 – – – 16 106 116 65 142 152
Endoplasmic Reticulum 3 0 3 3 3 2 1 0 0 0 0 6 1 0 2 – – – 3 0 5 1 0 3
Endosome 0 0 1 0 1 2 3 1 0 0 1 0 0 0 0 – – – 2 4 13 2 1 1
Golgi 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 – – – 1 0 0 0 0 2
Mitochondria 1 6 0 1 0 3 6 10 34 38 32 46 0 1 1 – – – 10 11 29 6 5 10
Nucleus 0 1 0 0 3 1 4 5 2 3 3 2 0 2 1 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 0 1 0 1 0 0 0 0 – – – 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – – – 1 0 1 0 0 1
SpindlePole 2 0 11 8 6 8 6 8 4 10 9 17 4 7 6 – – – 0 1 2 2 1 0
Vac/Vac Membrane 1 2 1 2 9 4 5 3 0 2 1 1 2 1 2 – – – 18 51 51 34 31 25
Unique Cell Count 29 215 336 282 493 417 570 618 454 462 382 484 79 205 245 82 233 341 157 230 242
Labelled Cell Count 33 229 364 310 520 437 603 656 495 504 429 536 84 229 263 82 233 341 157 230 242


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 3.3 5.2 5.1 5.6 4.8 4.7 4.8 4.1 4.1 4.1 3.9 5.1 5.2 4.9 3.5 4.5 4.5 5.9 5.8 6.1
Std Deviation (1e-4) 1.1 0.6 1.4 2.0 1.8 1.6 1.6 1.5 1.6 1.2 1.6 0.7 1.1 1.4 1.4 0.9 1.7 1.1 1.7 1.9 2.6
Intensity Change (Log2) – – – -0.02 0.13 -0.11 -0.15 -0.09 -0.35 -0.35 -0.32 -0.39 -0.01 0.01 -0.09 -0.55 -0.19 -0.21 0.18 0.17 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.5017 -0.4587 -0.9837 -0.884 -1.38 -0.9136 1.2951 2.9923 1.8284 1.7047 1.9695 1.9607 -2.1044 -1.1027 -1.5811 -1.3028 -1.8685 -1.4513
Actin 0.0101 0.002 0.0003 0.0007 0.0003 0.0013 0.0327 0 0.0042 0.0016 0.0786 0.0044 0.0028 0.0001 0.0016 0.0024 0.0013 0.0023
Bud 0.0018 0.0015 0.0002 0.0008 0.0002 0.0018 0.001 0.0009 0.0017 0.0028 0.0051 0.0003 0.0003 0.0009 0.002 0.0001 0.0018 0.0003
Bud Neck 0.0009 0.0003 0.0002 0.0001 0.0001 0.0012 0.0021 0.0002 0.0027 0.0004 0.0012 0.0172 0.0014 0.0004 0.0004 0.0003 0.0002 0.0059
Bud Periphery 0.0041 0.0017 0.0004 0.0014 0.0005 0.0008 0.0022 0.0009 0.003 0.0054 0.0174 0.0006 0.0008 0.0012 0.0034 0.0001 0.0031 0.0005
Bud Site 0.0015 0.022 0.0003 0.0003 0.0001 0.0002 0.0068 0.0012 0.0072 0.0009 0.0014 0.0007 0.0016 0.0103 0.0044 0.0003 0.0022 0.0001
Cell Periphery 0.0003 0.0006 0.0002 0.0002 0.0001 0.0001 0.0008 0.0001 0.0006 0.0001 0.0003 0.0002 0.0002 0.0002 0.0002 0.0001 0 0.0001
Cytoplasm 0.0684 0.1211 0.1178 0.0743 0.0935 0.1454 0.054 0.1196 0.0758 0.0384 0.0493 0.1241 0.0852 0.1963 0.146 0.0851 0.1201 0.2109
Cytoplasmic Foci 0.0151 0.0162 0.0324 0.0319 0.021 0.0378 0.0309 0.0311 0.0226 0.0293 0.0268 0.0224 0.0289 0.03 0.0428 0.0249 0.049 0.0347
Eisosomes 0.0004 0.0003 0.0002 0.0007 0.0003 0.0001 0.0006 0.0001 0.0005 0.0001 0.0007 0.0001 0.0001 0.0001 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0018 0.0015 0.0019 0.0016 0.0007 0.001 0.0029 0.0011 0.0013 0.0004 0.0018 0.0014 0.0024 0.003 0.0016 0.004 0.0006 0.0014
Endosome 0.0116 0.0287 0.0281 0.0284 0.013 0.0155 0.0182 0.0086 0.0115 0.0067 0.0271 0.0152 0.0324 0.0171 0.0211 0.0199 0.0212 0.0205
Golgi 0.0009 0.0033 0.001 0.0008 0.0002 0.0005 0.0041 0.0002 0.0018 0.0007 0.0064 0.0038 0.0027 0.0006 0.0008 0.0009 0.0014 0.0008
Lipid Particles 0.0012 0.002 0.003 0.0018 0.0017 0.0021 0.0084 0.001 0.0079 0.0007 0.0163 0.0035 0.0095 0.0012 0.0032 0.0017 0.0026 0.0027
Mitochondria 0.0007 0.0056 0.0072 0.0022 0.0017 0.0004 0.008 0.0005 0.0022 0.0017 0.0075 0.0046 0.0014 0.0004 0.0015 0.0006 0.002 0.0005
None 0.871 0.7597 0.7824 0.8354 0.8481 0.778 0.7974 0.8228 0.8287 0.8957 0.7272 0.7833 0.8112 0.7213 0.7521 0.8369 0.74 0.7064
Nuclear Periphery 0.0015 0.0009 0.0015 0.0011 0.0006 0.0005 0.0045 0.0004 0.0015 0.0005 0.0035 0.0017 0.0027 0.0009 0.0011 0.0064 0.0004 0.0007
Nucleolus 0.0005 0.0006 0.0012 0.0005 0.0005 0.0004 0.0009 0.0003 0.001 0.0005 0.0006 0.0006 0.0009 0.0004 0.0006 0.0005 0.0006 0.0002
Nucleus 0.0009 0.002 0.0029 0.0012 0.0012 0.0032 0.002 0.0008 0.0013 0.0007 0.002 0.0011 0.0015 0.0014 0.0013 0.0033 0.0013 0.0008
Peroxisomes 0.0003 0.0006 0.0014 0.0006 0.0004 0.0012 0.0117 0.0001 0.0149 0.0057 0.015 0.007 0.0029 0.0002 0.0023 0.0005 0.0381 0.0022
Punctate Nuclear 0.0012 0.0006 0.0023 0.0008 0.0005 0.0015 0.0027 0.0007 0.0022 0.0031 0.0049 0.0031 0.0025 0.0009 0.0018 0.0065 0.0022 0.001
Vacuole 0.0053 0.027 0.013 0.0131 0.0142 0.0063 0.0072 0.0087 0.0063 0.0038 0.0058 0.0042 0.0071 0.0122 0.0106 0.0047 0.0108 0.0075
Vacuole Periphery 0.0006 0.0018 0.002 0.002 0.0012 0.0005 0.0012 0.0008 0.0009 0.0007 0.0011 0.0006 0.0013 0.0008 0.0007 0.0007 0.0011 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 340.3221 97.0 20.5652 41.3363 253.0521 153.5179 62.0939 36.9661 26.7003 275.4065
Translational Efficiency 2.1095 1.3161 1.3387 1.084 1.885 4.4391 1.9799 1.294 1.796 1.7959

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
24 696 2039 1886 2058 1370 1509 1746 2082 2066 3548 3632

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 297.94 776.79 795.18 775.77 637.19 787.43 806.51 768.38 633.28 783.85 800.00 772.22
Standard Deviation 13.70 89.81 85.09 116.23 84.34 89.84 95.26 102.65 91.35 89.97 89.73 109.98
Intensity Change Log 2 — 1.382503 1.416260 1.380607 — 0.305428 0.339969 0.270096 — 0.742205 0.776356 0.723574

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000038 0.003408 0.000092 0.002109 0.000692 0.000449 0.000469 0.001079 0.000684 0.001446 0.000252 0.001614
Bud Neck 0.000007 0.066404 0.006261 0.027773 0.076327 0.082677 0.008262 0.012347 0.075448 0.077195 0.007112 0.020357
Bud Site 0.000012 0.065871 0.008256 0.059063 0.043275 0.030339 0.014874 0.041942 0.042776 0.042309 0.011071 0.050832
Cell Periphery 0.000050 0.000645 0.000211 0.000703 0.000436 0.000424 0.000225 0.000312 0.000431 0.000499 0.000217 0.000515
Cytoplasm 0.000021 0.350050* 0.467684* 0.468824* 0.478708* 0.446785* 0.474849* 0.576566* 0.473190* 0.414197* 0.470731* 0.520618*
Cytoplasmic Foci 0.000010 0.233386* 0.019798 0.034882 0.158751 0.157432 0.022623 0.050864 0.156921 0.183020* 0.020999 0.042565
Eisosomes 0.000046 0.000740 0.000050 0.000136 0.000576 0.000480 0.000059 0.000103 0.000570 0.000568 0.000054 0.000120
Endoplasmic Reticulum 0.000001 0.001201 0.000893 0.002487 0.000655 0.001053 0.001346 0.001243 0.000647 0.001103 0.001086 0.001889
Endosome 0.000001 0.034281 0.001646 0.008478 0.010422 0.017067 0.002698 0.003727 0.010302 0.022866 0.002093 0.006194
Golgi 0.000004 0.006640 0.000085 0.003308 0.002508 0.004742 0.000438 0.000531 0.002479 0.005381 0.000235 0.001973
Lipid Particles 0.000025 0.006656 0.001213 0.002452 0.006495 0.005275 0.001262 0.001472 0.006420 0.005740 0.001234 0.001981
Mitochondria 0.000008 0.002933 0.000633 0.006761 0.002664 0.008302 0.000498 0.000899 0.002633 0.006493 0.000575 0.003943
Mitotic Spindle 0.000304 0.003029 0.004358 0.021563 0.000207 0.004669 0.007463 0.004012 0.000208 0.004117 0.005678 0.013126
None 0.999348* 0.000572 0.005570 0.005406 0.005179 0.002649 0.005391 0.004501 0.016639 0.001950 0.005494 0.004971
Nuclear Periphery 0.000000 0.000256 0.001199 0.001012 0.000199 0.000207 0.001142 0.000587 0.000197 0.000223 0.001175 0.000808
Nuclear Periphery Foci 0.000016 0.000633 0.000298 0.000729 0.000328 0.000178 0.000623 0.000883 0.000324 0.000331 0.000436 0.000803
Nucleolus 0.000020 0.001027 0.000583 0.000560 0.001036 0.001153 0.000501 0.000568 0.001024 0.001111 0.000548 0.000564
Nucleus 0.000008 0.065334 0.439693* 0.196575* 0.134556* 0.114346 0.392458* 0.169453* 0.133005* 0.097835 0.419603* 0.183537*
Peroxisomes 0.000081 0.029605 0.000764 0.003611 0.004141 0.006789 0.000934 0.002702 0.004094 0.014475 0.000836 0.003174
Vacuole 0.000000 0.126138 0.040552 0.151026 0.072258 0.113317 0.063687 0.125588 0.071425 0.117636 0.050391 0.138797
Vacuole Periphery 0.000000 0.001189 0.000163 0.002542 0.000590 0.001667 0.000199 0.000624 0.000583 0.001506 0.000178 0.001620

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.51 -4.33 -3.99 2.75 -3.74 2.64 1.61 -1.43 -2.88 -2.37 -3.38 4.53 -2.45 0.79 -4.56
Bud Neck -13.02 -9.91 -11.65 8.42 -7.32 -1.25 17.20 16.38 17.45 -2.26 -0.36 17.77 15.09 18.45 -7.35
Bud Site -11.43 -6.87 -13.48 3.98 -10.02 3.46 9.31 2.21 -1.03 -6.52 0.17 11.11 1.65 1.57 -11.90
Cell Periphery -8.37 -9.86 -5.75 1.75 -3.68 0.19 6.34 4.81 3.01 -1.90 -1.38 7.78 1.31 2.31 -4.16
Cytoplasm -35.94* -67.68* -61.18* -5.32 2.60 3.41 6.30 -5.40 -8.00 -10.69 7.12 5.79 0.79 -6.92 -5.51
Cytoplasmic Foci -30.02* -19.49 -21.73 25.68 -6.66 0.27 35.30 25.89 20.84 -11.33 -4.60 36.49 30.34 32.93 -13.11
Eisosomes -5.06 -0.55 -4.76 4.54 -8.13 1.41 11.94 11.08 7.09 -3.98 0.09 12.15 10.90 7.89 -8.86
Endoplasmic Reticulum -9.68 -17.27 -5.04 -2.45 -2.29 -1.47 -11.84 -6.75 -1.69 4.11 -2.44 -11.42 -5.12 -2.88 -1.21
Endosome -11.63 -17.18 -9.57 9.44 -6.50 -5.15 10.76 10.14 11.93 -2.23 -8.88 12.61 7.92 14.06 -6.97
Golgi -6.27 -5.95 -3.93 4.14 -3.75 -3.39 7.74 10.15 6.73 -0.46 -4.97 11.45 4.44 7.01 -3.68
Lipid Particles -7.45 -17.10 -8.17 4.78 -3.27 1.73 9.85 9.52 6.89 -0.67 1.03 10.13 8.72 7.94 -3.31
Mitochondria -5.20 -7.64 -4.70 -1.09 -3.91 -5.02 5.52 4.12 6.96 -1.01 -4.68 5.28 0.34 4.72 -4.01
Mitotic Spindle -2.22 -4.91 -7.95 -5.55 -4.74 -3.72 -4.55 -5.06 0.23 1.94 -4.35 -6.67 -9.23 -4.42 -2.67
None 5750.57* 1652.65* 1608.46* -5.50 1.35 2.96 0.65 1.62 -1.50 0.99 5.99 4.77 5.19 -3.70 1.72
Nuclear Periphery -5.61 -26.57 -22.49 -16.61 -2.18 -0.24 -23.88 -11.94 -11.89 9.77 -0.98 -30.27 -21.75 -21.20 3.78
Nuclear Periphery Foci -7.09 -5.93 -9.76 -0.79 -2.84 3.23 -8.00 -6.65 -7.81 -2.00 -0.40 -5.22 -8.30 -7.41 -3.60
Nucleolus -2.38 -10.43 -3.18 0.39 -0.95 -0.54 3.77 3.12 2.61 -0.23 -0.41 3.43 1.80 1.79 -0.93
Nucleus -15.76 -68.48 -42.64 -23.86 26.21 4.06 -34.18 -8.53 -11.53 25.64 7.90 -50.93 -15.32 -23.63 36.94
Peroxisomes -9.16 -1.75 -4.32 8.26 -2.78 -5.23 10.32 3.36 6.74 -2.88 -8.85 10.55 3.80 9.97 -3.91
Vacuole -21.92 -23.19 -38.92 -11.10 -27.05 -8.36 -6.42 -17.42 -8.68 -9.75 -11.32 -1.22 -27.85 -15.05 -26.66
Vacuole Periphery -11.16 -11.58 -3.87 -0.64 -3.42 -4.54 8.80 0.53 4.23 -2.39 -5.63 11.64 -1.96 2.01 -4.03
Standard name
Human Ortholog
Description Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (38%), vacuole (2%), mixed (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Svs1

Svs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Svs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available