Standard name | |
---|---|
Human Ortholog | |
Description | Kinesin-related motor protein involved in mitotic spindle positioning; stabilizes microtubules by targeting Bik1p to the plus end; functions as a microtubule polymerase and catastrophe inhibitor in vitro; Kip2p levels are controlled during the cell cycle |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 14 | 14 | 55 | 34 | 28 | 10 | 41 | 26 | 9 | – | 6 | 18 | 4 | 6 | 1 | 19 | 4 | 5 | 19 | 31 | 13 | 4 | 16 | 25 |
Bud | 20 | 16 | 19 | 15 | 16 | 30 | 47 | 33 | 27 | – | 28 | 35 | 4 | 4 | 6 | 4 | 1 | 0 | 11 | 19 | 6 | 6 | 16 | 26 |
Bud Neck | 4 | 1 | 28 | 10 | 14 | 18 | 15 | 23 | 9 | – | 6 | 18 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 41 | 21 | 8 | 9 | 15 |
Bud Site | 5 | 3 | 12 | 16 | 4 | 14 | 13 | 9 | 16 | – | 13 | 16 | 3 | 1 | 2 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | – | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 33 | 62 | 42 | 72 | 84 | 57 | 84 | 82 | 43 | – | 41 | 31 | 10 | 39 | 37 | 8 | 2 | 0 | 13 | 32 | 23 | 4 | 14 | 19 |
Endoplasmic Reticulum | 3 | 8 | 6 | 7 | 4 | 6 | 4 | 3 | 8 | – | 1 | 11 | 2 | 4 | 3 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Endosome | 48 | 37 | 5 | 6 | 3 | 16 | 9 | 7 | 2 | – | 2 | 3 | 14 | 43 | 42 | 17 | 2 | 4 | 52 | 60 | 53 | 1 | 11 | 12 |
Golgi | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 16 | 3 | 6 | 1 | 1 | 2 | 0 | 2 | 0 |
Mitochondria | 54 | 83 | 8 | 43 | 18 | 107 | 135 | 92 | 198 | – | 185 | 110 | 30 | 8 | 15 | 7 | 3 | 1 | 24 | 46 | 68 | 6 | 19 | 23 |
Nucleus | 2 | 2 | 7 | 5 | 1 | 7 | 12 | 5 | 3 | – | 7 | 12 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | – | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 8 | 3 | 0 | 0 | 1 | 5 | 8 | 3 | 3 | – | 3 | 4 | 3 | 0 | 3 | 2 | 1 | 0 | 1 | 4 | 12 | 0 | 0 | 0 |
Peroxisomes | 21 | 15 | 2 | 8 | 4 | 9 | 4 | 6 | 5 | – | 4 | 3 | 9 | 28 | 32 | 5 | 0 | 2 | 11 | 10 | 11 | 1 | 10 | 9 |
SpindlePole | 49 | 19 | 124 | 74 | 71 | 80 | 99 | 134 | 47 | – | 45 | 78 | 8 | 24 | 22 | 22 | 2 | 6 | 75 | 58 | 103 | 3 | 17 | 26 |
Vac/Vac Membrane | 12 | 11 | 6 | 1 | 2 | 4 | 4 | 3 | 4 | – | 3 | 2 | 3 | 2 | 1 | 6 | 2 | 2 | 7 | 14 | 7 | 1 | 1 | 1 |
Unique Cell Count | 211 | 232 | 234 | 233 | 207 | 279 | 345 | 334 | 275 | 272 | 232 | 75 | 132 | 135 | 85 | 16 | 21 | 260 | 339 | 347 | 41 | 124 | 171 | |
Labelled Cell Count | 274 | 276 | 317 | 294 | 251 | 366 | 476 | 426 | 374 | 347 | 343 | 91 | 162 | 165 | 114 | 21 | 27 | 260 | 339 | 347 | 41 | 124 | 171 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.4 | 5.5 | 5.0 | 5.5 | 4.3 | 4.4 | 4.7 | 3.9 | – | 3.6 | 4.1 | 5.0 | 5.5 | 5.5 | 9.1 | 8.9 | 9.1 | – | 5.0 | 5.1 |
Std Deviation (1e-4) | 0.7 | 0.8 | 0.6 | 0.8 | 0.9 | 1.0 | 0.7 | 1.0 | 1.0 | – | 0.9 | 0.7 | 0.7 | 1.0 | 1.3 | 1.2 | 1.1 | 0.9 | – | 1.7 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.01 | -0.35 | -0.32 | -0.24 | -0.51 | – | -0.62 | -0.44 | -0.14 | -0.01 | 0.01 | 0.71 | 0.7 | 0.72 | – | -0.15 | -0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.5 | -2.7 | 0 | 0 | 0 | 0 | – | 0 | 0 | -3.5 | -4.7 | -5.9 | -0.2 | 0 | 0 |
Bud | -0.7 | -0.2 | 0 | 0 | 0 | 0 | – | 0 | 0 | -0.8 | -1.9 | -1.4 | -1.0 | 0 | 0 |
Bud Neck | -3.0 | -1.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | -3.1 | -4.1 | -4.2 | -3.3 | 0 | 0 |
Bud Site | 0.8 | -1.8 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | -1.8 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.3 | 5.3 | 0.7 | 1.8 | 1.9 | -0.7 | – | -0.9 | -1.4 | -0.9 | 2.6 | 2.1 | -1.9 | 0 | 0 |
Endoplasmic Reticulum | 0.3 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.3 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 8.3 | 8.0 | 5.6 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.2 | 2.3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 8.4 | 1.2 | 2.9 | 0 | 0 | 0 |
Nucleus | -0.6 | 0 | -0.3 | 0.3 | 0 | 0 | – | -0.3 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 6.8 | 7.3 | 0 | 0 | 0 |
SpindlePole | -4.6 | -3.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | -6.4 | -6.5 | -6.9 | -4.3 | -3.1 | -2.1 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.7596 | -0.2324 | -0.3933 | -0.0445 | -0.2381 | -0.2944 | 0.1481 | 0.8703 | 0.8587 | 1.1295 | 1.0292 | 0.8487 | -0.21 | 0.0467 | 0.1416 | 0.4022 | 0.4114 | 0.1336 |
Actin | 0.0356 | 0.0181 | 0.0303 | 0.0175 | 0.0264 | 0.0177 | 0.04 | 0.0348 | 0.0322 | 0.0158 | 0.0344 | 0.0135 | 0.014 | 0.0246 | 0.0231 | 0.0678 | 0.0033 | 0.0186 |
Bud | 0.0008 | 0.0026 | 0.002 | 0.0058 | 0.0015 | 0.0009 | 0.0009 | 0.0061 | 0.0038 | 0.0012 | 0.0018 | 0.0009 | 0.0005 | 0.0023 | 0.0023 | 0.0008 | 0.0017 | 0.0006 |
Bud Neck | 0.0151 | 0.0035 | 0.0426 | 0.0623 | 0.0032 | 0.0389 | 0.0062 | 0.006 | 0.0797 | 0.12 | 0.0078 | 0.0342 | 0.0075 | 0.0106 | 0.0833 | 0.0573 | 0.0008 | 0.0293 |
Bud Periphery | 0.0009 | 0.0029 | 0.0031 | 0.0042 | 0.0016 | 0.0014 | 0.0016 | 0.0053 | 0.0047 | 0.0012 | 0.0026 | 0.001 | 0.0006 | 0.0023 | 0.0032 | 0.0022 | 0.0014 | 0.0019 |
Bud Site | 0.0164 | 0.0645 | 0.0339 | 0.0085 | 0.0027 | 0.0029 | 0.0116 | 0.1042 | 0.0462 | 0.0114 | 0.0042 | 0.004 | 0.0212 | 0.0842 | 0.0165 | 0.0017 | 0.0016 | 0.0013 |
Cell Periphery | 0.0005 | 0.0006 | 0.0006 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0009 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0008 | 0.0005 | 0.001 | 0.0005 | 0.0001 | 0.0002 |
Cytoplasm | 0.0342 | 0.1917 | 0.1179 | 0.1159 | 0.0136 | 0.0411 | 0.0262 | 0.1014 | 0.1066 | 0.0606 | 0.0353 | 0.0404 | 0.0354 | 0.1576 | 0.1087 | 0.0119 | 0.033 | 0.0348 |
Cytoplasmic Foci | 0.323 | 0.2704 | 0.3311 | 0.3646 | 0.3109 | 0.3478 | 0.3511 | 0.2736 | 0.3249 | 0.3814 | 0.2823 | 0.3046 | 0.3372 | 0.2567 | 0.37 | 0.3556 | 0.3633 | 0.3513 |
Eisosomes | 0.0008 | 0.0004 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0011 | 0.0006 | 0 | 0.0003 |
Endoplasmic Reticulum | 0.0066 | 0.0032 | 0.0033 | 0.0042 | 0.0024 | 0.0027 | 0.0015 | 0.0027 | 0.0026 | 0.0016 | 0.0023 | 0.0007 | 0.0039 | 0.0034 | 0.0033 | 0.0011 | 0.0005 | 0.0009 |
Endosome | 0.1341 | 0.0804 | 0.1075 | 0.0994 | 0.146 | 0.086 | 0.1132 | 0.1065 | 0.0835 | 0.0739 | 0.1506 | 0.0833 | 0.1375 | 0.0702 | 0.08 | 0.0923 | 0.1938 | 0.0556 |
Golgi | 0.0287 | 0.0157 | 0.0219 | 0.03 | 0.0488 | 0.0315 | 0.0357 | 0.027 | 0.0248 | 0.0245 | 0.066 | 0.0349 | 0.026 | 0.0132 | 0.0212 | 0.0222 | 0.0839 | 0.0239 |
Lipid Particles | 0.1013 | 0.0323 | 0.0259 | 0.0186 | 0.0702 | 0.0671 | 0.0674 | 0.0136 | 0.0197 | 0.0229 | 0.0199 | 0.0559 | 0.0894 | 0.0416 | 0.0238 | 0.0089 | 0.0262 | 0.0793 |
Mitochondria | 0.0103 | 0.0166 | 0.0238 | 0.0067 | 0.0148 | 0.034 | 0.0241 | 0.0065 | 0.0157 | 0.0132 | 0.0323 | 0.0266 | 0.0177 | 0.0118 | 0.0124 | 0.054 | 0.0132 | 0.0246 |
None | 0.1123 | 0.1581 | 0.1343 | 0.1131 | 0.0327 | 0.0376 | 0.0635 | 0.0589 | 0.0778 | 0.0394 | 0.0154 | 0.0183 | 0.0675 | 0.2104 | 0.1367 | 0.1122 | 0.0126 | 0.0539 |
Nuclear Periphery | 0.0115 | 0.0018 | 0.002 | 0.0023 | 0.002 | 0.0029 | 0.0049 | 0.0043 | 0.0029 | 0.0009 | 0.0031 | 0.0009 | 0.0153 | 0.0009 | 0.0017 | 0.0009 | 0.0004 | 0.0003 |
Nucleolus | 0.0037 | 0.0017 | 0.001 | 0.0004 | 0.0005 | 0.0015 | 0.0047 | 0.0015 | 0.0012 | 0.0005 | 0.0003 | 0.0006 | 0.0016 | 0.0007 | 0.001 | 0.0007 | 0.0005 | 0.0008 |
Nucleus | 0.0021 | 0.0057 | 0.0023 | 0.0012 | 0.0007 | 0.0023 | 0.006 | 0.0019 | 0.0016 | 0.0006 | 0.0011 | 0.0008 | 0.0062 | 0.0013 | 0.0017 | 0.0006 | 0.0005 | 0.0005 |
Peroxisomes | 0.1406 | 0.1158 | 0.0895 | 0.1335 | 0.3132 | 0.2632 | 0.2162 | 0.2236 | 0.155 | 0.2183 | 0.3301 | 0.3663 | 0.1885 | 0.0934 | 0.0928 | 0.1577 | 0.2507 | 0.3041 |
Punctate Nuclear | 0.0133 | 0.0062 | 0.0165 | 0.0073 | 0.0054 | 0.0132 | 0.0182 | 0.0158 | 0.0121 | 0.0084 | 0.0035 | 0.0102 | 0.0122 | 0.01 | 0.0062 | 0.0454 | 0.0015 | 0.0157 |
Vacuole | 0.0063 | 0.0067 | 0.0082 | 0.0033 | 0.0017 | 0.0051 | 0.0049 | 0.0042 | 0.0034 | 0.0028 | 0.0041 | 0.0018 | 0.0147 | 0.0035 | 0.0083 | 0.0043 | 0.0092 | 0.0015 |
Vacuole Periphery | 0.0021 | 0.001 | 0.0018 | 0.0009 | 0.001 | 0.0014 | 0.0015 | 0.001 | 0.001 | 0.0012 | 0.0025 | 0.0009 | 0.0021 | 0.0005 | 0.0015 | 0.0014 | 0.0018 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.0231 | 19.5322 | 12.7683 | 13.4138 | 13.1527 | 8.319 | 21.6298 | 23.9609 | 16.5661 | 12.9163 |
Translational Efficiency | 0.9409 | 0.7318 | 0.9388 | 0.7606 | 0.5722 | 0.7516 | 0.7609 | 0.6075 | 0.7492 | 0.6603 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1695 | 1226 | 1842 | 1207 | 1857 | 417 | 50 | 249 | 3552 | 1643 | 1892 | 1456 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 718.77 | 786.86 | 1037.22 | 1011.49 | 767.61 | 806.23 | 1092.55 | 1057.60 | 744.30 | 791.78 | 1038.68 | 1019.38 |
Standard Deviation | 79.61 | 100.67 | 125.99 | 130.15 | 82.88 | 101.75 | 139.08 | 122.92 | 84.92 | 101.30 | 126.66 | 130.11 |
Intensity Change Log 2 | — | 0.130577 | 0.529120 | 0.492880 | — | 0.070818 | 0.509254 | 0.462349 | — | 0.100025 | 0.518895 | 0.477193 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001742 | 0.003589 | 0.009444 | 0.013300 | 0.003268 | 0.002480 | 0.003974 | 0.003773 | 0.002540 | 0.003308 | 0.009299 | 0.011671 |
Bud Neck | 0.055020 | 0.031383 | 0.018678 | 0.016462 | 0.046437 | 0.038180 | 0.022980 | 0.018393 | 0.050533 | 0.033108 | 0.018791 | 0.016792 |
Bud Site | 0.063060 | 0.055308 | 0.139372* | 0.234189* | 0.051346 | 0.058061 | 0.112858* | 0.206695* | 0.056936 | 0.056007 | 0.138671* | 0.229487* |
Cell Periphery | 0.000261 | 0.000165 | 0.000094 | 0.000144 | 0.000233 | 0.000428 | 0.000262 | 0.000088 | 0.000246 | 0.000232 | 0.000099 | 0.000135 |
Cytoplasm | 0.072872 | 0.078614 | 0.085237 | 0.117100* | 0.068267 | 0.074621 | 0.058405 | 0.077913 | 0.070465 | 0.077600 | 0.084528 | 0.110399* |
Cytoplasmic Foci | 0.267948* | 0.289366* | 0.093532 | 0.106058 | 0.282248* | 0.260679* | 0.078120 | 0.126813 | 0.275424* | 0.282085* | 0.093125 | 0.109608 |
Eisosomes | 0.000119 | 0.000128 | 0.000037 | 0.000038 | 0.000139 | 0.000131 | 0.000122 | 0.000029 | 0.000130 | 0.000129 | 0.000040 | 0.000037 |
Endoplasmic Reticulum | 0.000229 | 0.000374 | 0.000713 | 0.000655 | 0.000438 | 0.000292 | 0.001734 | 0.000209 | 0.000338 | 0.000353 | 0.000740 | 0.000579 |
Endosome | 0.106712 | 0.117319 | 0.030754 | 0.060582 | 0.138420 | 0.175028 | 0.079940 | 0.068554 | 0.123289 | 0.131966 | 0.032054 | 0.061945 |
Golgi | 0.063363 | 0.062090 | 0.008971 | 0.012942 | 0.061880 | 0.055563 | 0.008267 | 0.011236 | 0.062588 | 0.060433 | 0.008952 | 0.012650 |
Lipid Particles | 0.014323 | 0.011618 | 0.002150 | 0.001636 | 0.012524 | 0.011042 | 0.029020 | 0.001113 | 0.013383 | 0.011472 | 0.002860 | 0.001547 |
Mitochondria | 0.066850 | 0.042428 | 0.005127 | 0.008892 | 0.060792 | 0.049799 | 0.007979 | 0.002659 | 0.063683 | 0.044298 | 0.005203 | 0.007826 |
Mitotic Spindle | 0.038680 | 0.062920 | 0.442392* | 0.291751* | 0.050832 | 0.054431 | 0.270796* | 0.307336* | 0.045033 | 0.060766 | 0.437858* | 0.294416* |
None | 0.002703 | 0.000668 | 0.000354 | 0.000133 | 0.000910 | 0.000383 | 0.000238 | 0.000067 | 0.001766 | 0.000596 | 0.000350 | 0.000122 |
Nuclear Periphery | 0.000076 | 0.000139 | 0.000300 | 0.000289 | 0.000123 | 0.000086 | 0.000416 | 0.000194 | 0.000100 | 0.000125 | 0.000303 | 0.000272 |
Nuclear Periphery Foci | 0.001123 | 0.000953 | 0.001798 | 0.001176 | 0.001241 | 0.000410 | 0.006386 | 0.000946 | 0.001185 | 0.000815 | 0.001920 | 0.001137 |
Nucleolus | 0.002601 | 0.001275 | 0.000838 | 0.000301 | 0.001969 | 0.001172 | 0.001951 | 0.000256 | 0.002270 | 0.001249 | 0.000867 | 0.000293 |
Nucleus | 0.003070 | 0.004134 | 0.007221 | 0.001217 | 0.005004 | 0.002802 | 0.015344 | 0.000707 | 0.004081 | 0.003796 | 0.007436 | 0.001130 |
Peroxisomes | 0.228623* | 0.218433* | 0.127353* | 0.084860 | 0.197793* | 0.172416* | 0.202898* | 0.118670* | 0.212505* | 0.206754* | 0.129349* | 0.090642 |
Vacuole | 0.008709 | 0.016729 | 0.024950 | 0.043164 | 0.013207 | 0.036997 | 0.095853 | 0.053040 | 0.011061 | 0.021873 | 0.026823 | 0.044853 |
Vacuole Periphery | 0.001918 | 0.002367 | 0.000684 | 0.005110 | 0.002928 | 0.005000 | 0.002456 | 0.001309 | 0.002446 | 0.003036 | 0.000731 | 0.004460 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.54 | -8.82 | -6.05 | -4.75 | -1.52 | 1.16 | -0.70 | -0.32 | -1.05 | 0.30 | -1.67 | -7.76 | -5.57 | -4.94 | -0.96 |
Bud Neck | 6.30 | 11.12 | 12.69 | 6.57 | 3.47 | 1.68 | 4.21 | 9.81 | 4.89 | 0.76 | 6.23 | 13.95 | 16.48 | 8.18 | 3.61 |
Bud Site | 1.62 | -12.55 | -18.70 | -19.60 | -9.52 | -0.97 | -2.76 | -8.27 | -7.34 | -2.30 | 0.24 | -15.50 | -21.67 | -20.87 | -9.62 |
Cell Periphery | 4.25 | 8.28 | 5.99 | 2.08 | -2.67 | -0.76 | -0.89 | 6.04 | 1.33 | 3.12 | 0.20 | 9.02 | 7.12 | 1.63 | -1.69 |
Cytoplasm | -0.94 | -4.89 | -9.22 | -7.99 | -5.21 | -0.70 | 0.36 | -2.84 | -2.00 | -1.88 | -1.51 | -6.09 | -10.46 | -8.30 | -4.85 |
Cytoplasmic Foci | -2.30 | 28.08 | 23.10 | 25.08 | -3.26 | 1.76 | 12.18 | 14.80 | 9.88 | -1.82 | -0.94 | 39.04 | 30.87 | 26.86 | -4.07 |
Eisosomes | -1.11 | 10.53 | 10.80 | 12.43 | 1.29 | 0.66 | -0.19 | 13.54 | 8.06 | 4.19 | -0.01 | 15.69 | 16.94 | 14.65 | 2.86 |
Endoplasmic Reticulum | -2.57 | -8.46 | -2.14 | -1.22 | 2.91 | 1.88 | -2.84 | 1.58 | -0.17 | 3.03 | -0.43 | -7.72 | -1.46 | -1.29 | 3.78 |
Endosome | -1.72 | 18.64 | 10.84 | 11.65 | -6.58 | -3.55 | 3.42 | 9.14 | 9.57 | 0.81 | -1.65 | 27.63 | 16.67 | 15.22 | -7.19 |
Golgi | 0.55 | 24.60 | 21.85 | 18.12 | -2.53 | 1.39 | 21.97 | 21.48 | 10.03 | -0.17 | 1.01 | 34.97 | 30.42 | 20.55 | -2.51 |
Lipid Particles | 1.39 | 8.12 | 8.82 | 7.56 | 3.75 | 0.53 | -0.75 | 10.77 | 3.56 | 1.44 | 1.31 | 10.38 | 13.46 | 8.22 | 3.31 |
Mitochondria | 4.91 | 15.96 | 14.77 | 10.19 | -1.63 | 1.57 | 11.44 | 17.24 | 7.37 | 1.33 | 5.11 | 22.53 | 20.91 | 12.28 | -1.18 |
Mitotic Spindle | -5.09 | -43.46* | -24.56 | -21.05 | 10.40 | -0.32 | -4.33 | -11.31 | -10.70 | -1.20 | -3.75 | -43.33 | -26.64 | -23.70 | 10.39 |
None | 3.73 | 4.28 | 4.92 | 2.35 | 7.14 | 2.85 | 2.07 | 6.16 | 2.38 | 1.44 | 3.98 | 4.94 | 6.30 | 2.76 | 7.66 |
Nuclear Periphery | -2.20 | -10.84 | -5.68 | -3.69 | 4.76 | 2.10 | -3.23 | -3.76 | -4.73 | 1.61 | -1.20 | -10.59 | -5.86 | -4.62 | 5.22 |
Nuclear Periphery Foci | 0.28 | -5.22 | -0.84 | -1.16 | 4.01 | 3.32 | -1.94 | -0.87 | -4.46 | 1.90 | 1.10 | -6.19 | -1.07 | -1.89 | 4.72 |
Nucleolus | 3.01 | 2.85 | 4.91 | 3.29 | 4.24 | 2.02 | -0.72 | 4.33 | 3.37 | 1.31 | 3.43 | 2.71 | 6.21 | 4.09 | 4.67 |
Nucleus | -1.45 | -8.08 | 1.56 | 2.23 | 8.53 | 2.46 | -1.50 | 3.95 | 1.59 | 1.88 | 0.21 | -7.49 | 3.84 | 2.47 | 8.80 |
Peroxisomes | 1.10 | 16.26 | 21.27 | 18.27 | 5.81 | 2.07 | 0.67 | 9.26 | 5.33 | 2.55 | 0.85 | 17.73 | 24.78 | 18.75 | 5.71 |
Vacuole | -4.93 | -14.39 | -14.89 | -12.20 | -4.68 | -5.51 | -3.48 | -6.91 | -4.68 | 0.68 | -6.73 | -14.32 | -16.04 | -12.38 | -5.24 |
Vacuole Periphery | -1.36 | 4.87 | -2.56 | -2.04 | -4.27 | -2.72 | 0.66 | 3.22 | 5.76 | 0.99 | -1.65 | 6.21 | -1.51 | -0.70 | -4.29 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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