Standard name | |
---|---|
Human Ortholog | |
Description | Vacuolar aspartyl protease (proteinase A); required for posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; plays a protective role in acetic acid induced apoptosis; synthesized as a zymogen, self-activates |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 27 | 44 | 40 | 29 | 53 | 85 | 107 | 90 | 123 | 86 | 109 | – | 68 | 80 | 10 | 9 | 7 | 1 | 5 | 3 | 1 | 3 | 3 |
Endoplasmic Reticulum | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | – | 3 | 0 | 5 | 4 | 4 | 0 | 0 | 2 | 0 | 0 | 0 |
Endosome | 0 | 4 | 26 | 10 | 8 | 2 | 0 | 2 | 0 | 0 | 0 | 1 | – | 7 | 2 | 0 | 1 | 0 | 0 | 3 | 2 | 3 | 5 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 51 | 15 | 16 | 8 | 27 | 55 | 51 | 25 | 16 | 45 | 30 | 34 | – | 0 | 0 | 4 | 1 | 0 | 1 | 1 | 2 | 0 | 3 | 3 |
Nucleus | 15 | 11 | 32 | 49 | 78 | 110 | 124 | 119 | 57 | 97 | 35 | 45 | – | 11 | 2 | 48 | 26 | 21 | 3 | 0 | 0 | 5 | 2 | 2 |
Nuclear Periphery | 2 | 3 | 1 | 1 | 3 | 6 | 4 | 1 | 4 | 8 | 1 | 5 | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 |
Nucleolus | 0 | 1 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 3 | 2 | 4 | – | 0 | 0 | 4 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 |
Vac/Vac Membrane | 43 | 83 | 172 | 138 | 185 | 267 | 285 | 290 | 242 | 324 | 170 | 256 | – | 49 | 32 | 55 | 34 | 38 | 80 | 111 | 226 | 58 | 55 | 77 |
Unique Cell Count | 85 | 117 | 232 | 191 | 265 | 394 | 432 | 414 | 324 | 448 | 245 | 355 | 121 | 102 | 102 | 63 | 58 | 92 | 127 | 249 | 74 | 76 | 101 | |
Labelled Cell Count | 121 | 146 | 294 | 250 | 335 | 497 | 551 | 545 | 411 | 602 | 324 | 456 | 138 | 116 | 129 | 76 | 72 | 92 | 127 | 249 | 74 | 76 | 101 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 5.2 | 5.4 | 5.8 | 6.6 | 6.1 | 6.2 | 6.2 | 6.3 | 6.0 | 5.8 | 6.4 | – | 6.2 | 5.6 | 9.7 | 10.1 | 9.3 | 5.4 | 6.9 | 7.1 |
Std Deviation (1e-4) | 0.7 | 1.4 | 1.7 | 2.2 | 1.8 | 1.9 | 1.8 | 1.8 | 1.8 | 1.7 | 1.7 | 2.1 | – | 1.5 | 1.5 | 3.0 | 3.5 | 3.1 | 0.9 | 1.6 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.27 | 0.17 | 0.19 | 0.18 | 0.21 | 0.15 | 0.1 | 0.24 | – | 0.19 | 0.04 | 0.83 | 0.89 | 0.77 | -0.01 | 0.34 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | -2.5 | -1.8 | 0.2 | 2.0 | 2.4 | 2.4 | 4.0 | 3.2 | – | 7.1 | 10.4 | -2.1 | -0.9 | -1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Endosome | -2.2 | -3.6 | -6.3 | -7.1 | -6.4 | -6.2 | -7.2 | -5.4 | -6.2 | – | -1.7 | -2.8 | -3.5 | -2.3 | -2.7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.2 | 1.3 | 2.7 | 2.0 | -0.4 | -1.0 | 1.4 | 2.0 | 1.1 | – | -3.0 | 0 | -1.1 | 0 | 0 |
Nucleus | 3.1 | 4.2 | 4.1 | 4.3 | 4.3 | 1.2 | 2.5 | 0.2 | -0.4 | – | -1.3 | -3.3 | 6.6 | 4.9 | 4.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.4 | -1.1 | -1.7 | -2.2 | -1.1 | 0.1 | -0.5 | -1.2 | -0.5 | – | -6.2 | -7.4 | -3.6 | -3.1 | -1.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.629 | 3.0252 | 1.4138 | 1.6442 | 2.2505 | 2.4508 | 2.3689 | 2.6418 | 2.0908 | 1.8593 | 2.5753 | 2.2541 | 0.9878 | 2.5306 | 2.2332 | 1.9524 | 2.2793 | 2.7861 |
Actin | 0.0026 | 0 | 0.0001 | 0 | 0.0505 | 0.0013 | 0.0584 | 0.0001 | 0.0043 | 0.0019 | 0.0311 | 0.0002 | 0.0521 | 0 | 0.0293 | 0.0566 | 0.0246 | 0 |
Bud | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0012 | 0.0003 | 0.0015 | 0.0003 | 0.0025 | 0.0006 | 0.0009 | 0.0003 | 0.0006 | 0.0002 | 0.0003 | 0.0003 |
Bud Neck | 0.0098 | 0.0004 | 0.0008 | 0.0007 | 0.0012 | 0.0017 | 0.0038 | 0.0011 | 0.0043 | 0.001 | 0.0034 | 0.0026 | 0.0022 | 0.0017 | 0.0017 | 0.0012 | 0.0025 | 0.0013 |
Bud Periphery | 0.0007 | 0.0007 | 0.0008 | 0.0006 | 0.0007 | 0.0014 | 0.0023 | 0.0009 | 0.0029 | 0.0009 | 0.0058 | 0.0013 | 0.0023 | 0.0008 | 0.0016 | 0.0006 | 0.0007 | 0.001 |
Bud Site | 0.0021 | 0.0005 | 0.0006 | 0.0005 | 0.0011 | 0.0008 | 0.0063 | 0.0012 | 0.0049 | 0.0008 | 0.0047 | 0.001 | 0.0021 | 0.0008 | 0.002 | 0.0006 | 0.0007 | 0.0005 |
Cell Periphery | 0.0036 | 0.0046 | 0.0051 | 0.0024 | 0.0024 | 0.0059 | 0.0024 | 0.0039 | 0.0037 | 0.0023 | 0.0045 | 0.0037 | 0.0042 | 0.0055 | 0.0056 | 0.0032 | 0.0044 | 0.0059 |
Cytoplasm | 0.0023 | 0.0007 | 0.001 | 0.0008 | 0.0048 | 0.0007 | 0.0064 | 0.0079 | 0.0053 | 0.0016 | 0.0077 | 0.0008 | 0.0132 | 0.0005 | 0.0009 | 0.0004 | 0.0019 | 0.0005 |
Cytoplasmic Foci | 0.0101 | 0.0011 | 0.019 | 0.0019 | 0.0386 | 0.0017 | 0.0201 | 0.0025 | 0.0094 | 0.0065 | 0.0105 | 0.0051 | 0.0239 | 0.0036 | 0.0096 | 0.0057 | 0.0256 | 0.0014 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.001 | 0 | 0.0002 | 0 | 0.0005 | 0 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0013 | 0.0012 | 0.0014 | 0.0006 | 0.0015 | 0.0008 | 0.0084 | 0.0022 | 0.0031 | 0.0019 | 0.0046 | 0.0015 | 0.0058 | 0.0007 | 0.002 | 0.0003 | 0.0067 | 0.0006 |
Endosome | 0.1207 | 0.0695 | 0.1045 | 0.1327 | 0.1574 | 0.0612 | 0.1897 | 0.0782 | 0.1534 | 0.131 | 0.1429 | 0.1087 | 0.0804 | 0.0544 | 0.1021 | 0.1021 | 0.0804 | 0.0574 |
Golgi | 0.0056 | 0.0019 | 0.0065 | 0.0028 | 0.0134 | 0.0047 | 0.0235 | 0.0032 | 0.0123 | 0.0107 | 0.0272 | 0.0043 | 0.0135 | 0.002 | 0.0111 | 0.0046 | 0.012 | 0.0014 |
Lipid Particles | 0.0497 | 0.0182 | 0.0297 | 0.0118 | 0.0418 | 0.0352 | 0.0454 | 0.0124 | 0.0297 | 0.0234 | 0.0206 | 0.0144 | 0.0751 | 0.0193 | 0.0281 | 0.051 | 0.0833 | 0.0159 |
Mitochondria | 0.0358 | 0.0107 | 0.0576 | 0.0407 | 0.0037 | 0.03 | 0.0596 | 0.0193 | 0.0752 | 0.0092 | 0.046 | 0.0075 | 0.0574 | 0.0143 | 0.067 | 0.0015 | 0.0036 | 0.0009 |
None | 0.0107 | 0.0004 | 0.0018 | 0.0001 | 0.0072 | 0.0001 | 0.0219 | 0.0008 | 0.0006 | 0.0002 | 0.0016 | 0.0002 | 0.0169 | 0.0001 | 0.0005 | 0.0001 | 0.0007 | 0.0001 |
Nuclear Periphery | 0.0028 | 0.0061 | 0.0238 | 0.0013 | 0.0069 | 0.0038 | 0.0385 | 0.0034 | 0.0227 | 0.0028 | 0.0161 | 0.0105 | 0.0249 | 0.0016 | 0.0076 | 0.0007 | 0.0408 | 0.0013 |
Nucleolus | 0.0057 | 0.007 | 0.0073 | 0.0033 | 0.0034 | 0.0068 | 0.007 | 0.0116 | 0.0091 | 0.0029 | 0.0105 | 0.0102 | 0.0113 | 0.011 | 0.0088 | 0.0064 | 0.0039 | 0.01 |
Nucleus | 0.0029 | 0.0052 | 0.0126 | 0.0018 | 0.0043 | 0.0198 | 0.0106 | 0.0046 | 0.008 | 0.0025 | 0.0253 | 0.0087 | 0.012 | 0.0033 | 0.0225 | 0.002 | 0.0051 | 0.0041 |
Peroxisomes | 0.0028 | 0.0005 | 0.0059 | 0.0007 | 0.0288 | 0.0015 | 0.0105 | 0.0007 | 0.0048 | 0.0129 | 0.0035 | 0.001 | 0.028 | 0.0022 | 0.014 | 0.0024 | 0.0191 | 0.0007 |
Punctate Nuclear | 0.0063 | 0.0002 | 0.0006 | 0 | 0.0223 | 0.0001 | 0.0078 | 0.0002 | 0.0005 | 0.0128 | 0.0034 | 0.0009 | 0.0082 | 0.0001 | 0.0029 | 0.0001 | 0.0184 | 0 |
Vacuole | 0.4888 | 0.6019 | 0.4532 | 0.5979 | 0.4651 | 0.6106 | 0.3203 | 0.6125 | 0.4423 | 0.6217 | 0.4969 | 0.6292 | 0.3769 | 0.625 | 0.4628 | 0.6054 | 0.5343 | 0.7183 |
Vacuole Periphery | 0.2354 | 0.2689 | 0.2675 | 0.1991 | 0.1441 | 0.2111 | 0.1548 | 0.2328 | 0.2019 | 0.1527 | 0.1308 | 0.1873 | 0.1884 | 0.253 | 0.219 | 0.1548 | 0.131 | 0.1786 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 393.1671 | 347.4182 | 348.5398 | 358.2313 | 234.9117 | 522.4236 | 469.3891 | 444.6061 | 518.9724 | 416.1761 |
Translational Efficiency | 1.1749 | 1.2004 | 1.2278 | 1.3873 | 1.3705 | 1.0957 | 1.2572 | 1.383 | 1.3348 | 1.4277 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
656 | 78 | 902 | 1359 | 1816 | 1899 | 325 | 1463 | 2472 | 1977 | 1227 | 2822 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 760.06 | 853.66 | 846.98 | 926.46 | 735.44 | 785.39 | 962.37 | 1023.52 | 741.97 | 788.08 | 877.54 | 976.78 |
Standard Deviation | 98.58 | 115.47 | 165.23 | 153.15 | 92.79 | 105.56 | 123.98 | 186.29 | 94.99 | 106.80 | 163.51 | 177.87 |
Intensity Change Log 2 | — | 0.167548 | 0.156215 | 0.285615 | — | 0.094802 | 0.387984 | 0.476860 | — | 0.132232 | 0.274844 | 0.382831 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000020 | 0.000973 | 0.000503 | 0.000550 | 0.000022 | 0.000048 | 0.000178 | 0.000513 | 0.000022 | 0.000085 | 0.000417 | 0.000531 |
Bud Neck | 0.020064 | 0.019768 | 0.041660 | 0.015538 | 0.027150 | 0.014998 | 0.035706 | 0.009874 | 0.025270 | 0.015186 | 0.040083 | 0.012601 |
Bud Site | 0.011011 | 0.015127 | 0.015855 | 0.029504 | 0.006519 | 0.009847 | 0.013022 | 0.032688 | 0.007711 | 0.010055 | 0.015105 | 0.031154 |
Cell Periphery | 0.000495 | 0.000369 | 0.000375 | 0.000098 | 0.000335 | 0.000162 | 0.000165 | 0.000562 | 0.000377 | 0.000170 | 0.000320 | 0.000338 |
Cytoplasm | 0.018514 | 0.032750 | 0.099530 | 0.029394 | 0.067644 | 0.036390 | 0.026238 | 0.015431 | 0.054606 | 0.036246 | 0.080117 | 0.022155 |
Cytoplasmic Foci | 0.009517 | 0.033950 | 0.024038 | 0.069455 | 0.024034 | 0.015016 | 0.028843 | 0.039443 | 0.020182 | 0.015763 | 0.025311 | 0.053896 |
Eisosomes | 0.000022 | 0.000038 | 0.000067 | 0.000020 | 0.000033 | 0.000022 | 0.000055 | 0.000016 | 0.000030 | 0.000022 | 0.000064 | 0.000018 |
Endoplasmic Reticulum | 0.001254 | 0.001134 | 0.004035 | 0.001193 | 0.003215 | 0.001881 | 0.003472 | 0.001100 | 0.002695 | 0.001851 | 0.003886 | 0.001145 |
Endosome | 0.069531 | 0.075541 | 0.109260 | 0.315972* | 0.066262 | 0.055473 | 0.198926 | 0.245012* | 0.067130 | 0.056265 | 0.133010 | 0.279184* |
Golgi | 0.001766 | 0.010484 | 0.001656 | 0.012374 | 0.000434 | 0.001444 | 0.001292 | 0.017868 | 0.000788 | 0.001800 | 0.001559 | 0.015222 |
Lipid Particles | 0.001606 | 0.002808 | 0.003111 | 0.003199 | 0.002496 | 0.001415 | 0.004906 | 0.001113 | 0.002260 | 0.001470 | 0.003586 | 0.002117 |
Mitochondria | 0.000868 | 0.010586 | 0.002916 | 0.004254 | 0.000470 | 0.003737 | 0.000957 | 0.004295 | 0.000576 | 0.004007 | 0.002397 | 0.004276 |
Mitotic Spindle | 0.000098 | 0.005678 | 0.006276 | 0.029867 | 0.000664 | 0.002393 | 0.007950 | 0.014308 | 0.000513 | 0.002522 | 0.006719 | 0.021801 |
None | 0.001337 | 0.000086 | 0.002329 | 0.001145 | 0.000974 | 0.000276 | 0.000193 | 0.000105 | 0.001071 | 0.000269 | 0.001763 | 0.000606 |
Nuclear Periphery | 0.000352 | 0.000349 | 0.002513 | 0.001181 | 0.000427 | 0.000621 | 0.002793 | 0.000874 | 0.000407 | 0.000610 | 0.002587 | 0.001022 |
Nuclear Periphery Foci | 0.001742 | 0.000424 | 0.001938 | 0.003998 | 0.000202 | 0.000243 | 0.004235 | 0.002157 | 0.000611 | 0.000250 | 0.002546 | 0.003044 |
Nucleolus | 0.000723 | 0.001313 | 0.019960 | 0.002842 | 0.001229 | 0.001989 | 0.017718 | 0.000978 | 0.001095 | 0.001962 | 0.019366 | 0.001876 |
Nucleus | 0.005862 | 0.008493 | 0.210897* | 0.004918 | 0.030063 | 0.025810 | 0.054627 | 0.001642 | 0.023641 | 0.025126 | 0.169505* | 0.003220 |
Peroxisomes | 0.000585 | 0.007984 | 0.005626 | 0.015120 | 0.001066 | 0.001305 | 0.009127 | 0.007708 | 0.000939 | 0.001568 | 0.006553 | 0.011277 |
Vacuole | 0.834664* | 0.749434* | 0.440596* | 0.436666* | 0.756833* | 0.804084* | 0.575658* | 0.545335* | 0.777487* | 0.801928* | 0.476370* | 0.493003* |
Vacuole Periphery | 0.019970 | 0.022712 | 0.006859 | 0.022711 | 0.009926 | 0.022849 | 0.013940 | 0.058978 | 0.012591 | 0.022844 | 0.008735 | 0.041513 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.77 | -3.10 | -2.18 | 0.90 | -0.08 | -2.25 | -3.79 | -3.21 | -3.04 | -1.85 | -2.53 | -3.52 | -3.65 | -3.11 | -0.55 |
Bud Neck | 0.12 | -6.71 | 1.28 | 0.58 | 8.46 | 5.55 | -2.15 | 8.37 | 3.41 | 6.48 | 5.47 | -5.96 | 7.25 | 1.18 | 11.20 |
Bud Site | -1.05 | -2.13 | -7.15 | -1.76 | -5.16 | -3.33 | -2.85 | -11.78 | -9.91 | -5.90 | -2.34 | -5.00 | -14.83 | -12.48 | -7.74 |
Cell Periphery | 0.62 | 1.12 | 5.77 | 1.39 | 1.73 | 5.30 | 2.50 | -1.67 | -3.08 | -2.71 | 6.43 | 0.87 | 0.65 | -2.40 | -0.36 |
Cytoplasm | -1.64 | -13.89 | -5.54 | -0.10 | 10.54 | 7.59 | 5.66 | 13.55 | 6.05 | 3.26 | 5.13 | -6.63 | 8.88 | 2.89 | 12.23 |
Cytoplasmic Foci | -3.31 | -6.48 | -17.90 | -4.06 | -12.75 | 4.96 | -1.63 | -6.15 | -10.35 | -2.23 | 2.79 | -3.01 | -15.61 | -17.58 | -11.23 |
Eisosomes | -1.44 | -8.54 | 0.35 | 1.61 | 11.79 | 2.28 | -5.54 | 7.12 | 1.08 | 9.01 | 1.65 | -10.13 | 5.92 | 0.81 | 14.97 |
Endoplasmic Reticulum | 0.49 | -12.03 | 0.16 | -0.40 | 12.38 | 4.42 | -1.55 | 6.80 | 3.56 | 6.92 | 3.49 | -5.45 | 6.92 | 4.01 | 14.09 |
Endosome | -0.41 | -3.55 | -24.94* | -15.54 | -21.28 | 3.11 | -9.65 | -24.42 | -26.43 | -4.34 | 3.39 | -9.03 | -35.95* | -38.23* | -20.42 |
Golgi | -1.44 | 0.34 | -6.00 | -0.13 | -7.80 | -3.78 | -3.59 | -12.71 | -11.73 | -11.95 | -2.31 | -1.31 | -14.54 | -13.15 | -13.69 |
Lipid Particles | -0.83 | -2.48 | -2.37 | -0.51 | -0.59 | 2.73 | -1.77 | 3.56 | 0.94 | 2.78 | 2.20 | -2.92 | -0.21 | -2.63 | 2.86 |
Mitochondria | -1.41 | -1.83 | -3.28 | 0.94 | -1.07 | -4.87 | -2.57 | -3.88 | -0.39 | -3.19 | -4.90 | -2.41 | -5.27 | -0.09 | -2.02 |
Mitotic Spindle | -1.36 | -4.28 | -10.57 | -5.40 | -6.07 | -1.90 | -3.16 | -7.25 | -6.27 | -1.32 | -2.42 | -5.04 | -12.59 | -11.03 | -5.38 |
None | 1.36 | -0.81 | 0.19 | -1.87 | 1.32 | 1.68 | 0.97 | 2.08 | 2.49 | 2.14 | 2.05 | -1.19 | 0.99 | -1.24 | 2.23 |
Nuclear Periphery | -0.03 | -8.91 | -4.53 | -4.76 | 4.22 | -1.56 | -4.98 | -2.05 | -1.14 | 3.96 | -1.67 | -10.77 | -4.91 | -3.36 | 6.53 |
Nuclear Periphery Foci | 1.08 | -1.16 | -2.21 | -5.48 | -1.36 | -0.34 | -3.19 | -4.67 | -4.51 | 1.80 | 1.12 | -4.25 | -5.45 | -7.33 | 0.42 |
Nucleolus | -0.79 | -9.98 | -5.83 | -2.68 | 8.44 | -1.69 | -5.94 | -0.03 | 1.69 | 5.94 | -2.12 | -11.46 | -4.03 | -0.77 | 10.70 |
Nucleus | -0.73 | -22.38 | -0.50 | 0.56 | 22.39 | 1.30 | -5.18 | 12.61 | 10.60 | 9.47 | -0.60 | -20.35 | 11.19 | 9.50 | 23.55 |
Peroxisomes | -1.93 | -6.04 | -11.38 | -1.60 | -6.52 | -0.91 | -4.79 | -7.06 | -6.80 | 0.73 | -2.38 | -7.28 | -13.01 | -11.82 | -4.60 |
Vacuole | 2.75 | 29.91 | 31.59 | 8.72 | -1.05 | -5.62 | 12.00 | 20.82 | 26.03 | 0.86 | -3.14 | 30.92 | 35.55 | 36.79 | -2.97 |
Vacuole Periphery | -0.36 | 7.03 | -0.27 | 0.27 | -11.16 | -9.76 | -2.05 | -17.80 | -12.09 | -15.46* | -7.60 | 5.81 | -16.32 | -8.83 | -20.12 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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