Standard name
Human Ortholog
Description Component of both the Rpd3S and Rpd3L histone deacetylase complexes; negative regulator of meiosis; required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p; UME1 has a paralog, WTM2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.47 0.29 0.33 0.53 0.68 0.58 0.65 0.63 0.64 0.64 0.6 0.7 0.68 0.75 0.76 0.07 0.05 0 0.32 0.23 0.2 0.47 0.43 0.38
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0.31 0.45 0.51 0.36 0.39 0.47 0.42 0.52 0.41 0.54 0.45 0.07 0.05 0 0.06 0.06 0.06 0.13 0.07 0.06 0.1 0.09 0.11
Nucleus 0.2 0.36 0.36 0.15 0.1 0.14 0.12 0.07 0 0.07 0.06 0.05 0.16 0.13 0.13 0.85 0.87 0.9 0.19 0.36 0.36 0.15 0.14 0.12
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.06 0.06 0.06 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.09 0.05 0.1 0.06
Vac/Vac Membrane 0.1 0.18 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0.05 0.13 0.14 0.13 0.07 0.07 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 3 4 7
Bud 3 0 2 3 1 1 3 10 6 7 4 5 4 2 1 0 0 0 2 4 0 4 14 17
Bud Neck 0 0 1 0 0 1 1 5 1 2 2 2 0 0 0 0 0 0 3 0 0 0 2 5
Bud Site 0 0 0 0 0 2 0 1 2 1 2 0 0 1 0 1 1 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 2 0 0 1 1 0 0 0 0 0 0 0 3
Cytoplasm 128 42 29 77 100 85 163 230 132 178 121 164 298 372 460 20 8 5 107 39 18 133 179 185
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 10 5 2 3 0 1 2 4 10
Endosome 13 4 0 1 0 0 0 0 0 0 0 0 24 30 27 2 1 1 14 2 2 8 8 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 3
Mitochondria 73 44 39 74 54 57 116 152 108 115 110 105 32 24 19 16 10 8 43 11 5 29 39 52
Nucleus 55 51 31 22 15 21 30 27 8 21 13 11 70 67 76 228 142 117 63 61 32 42 58 57
Nuclear Periphery 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 4 2 0 0 0 0 0 2 2
Nucleolus 22 11 3 1 1 6 8 3 0 6 5 3 13 11 11 11 8 4 19 10 5 17 17 21
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0
SpindlePole 4 2 5 1 1 3 4 5 2 2 3 3 3 3 5 1 0 1 23 6 8 15 40 30
Vac/Vac Membrane 26 26 1 1 5 4 2 4 3 7 1 0 41 24 25 10 6 6 45 23 11 20 30 61
Unique Cell Count 272 143 87 146 148 147 249 364 207 280 202 233 441 497 605 267 164 130 341 169 90 287 415 486
Labelled Cell Count 325 181 111 180 177 180 327 437 265 339 261 295 485 536 626 306 184 144 341 169 90 287 415 486


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.3 3.6 3.3 3.6 3.1 2.7 3.2 2.8 2.6 2.7 2.5 4.3 4.3 4.1 7.0 8.3 8.6 4.3 4.5 5.0
Std Deviation (1e-4) 1.1 1.5 0.8 1.0 1.3 1.3 1.2 1.3 1.4 1.2 1.1 1.2 1.5 1.5 1.5 2.3 2.5 2.5 1.7 2.0 2.1
Intensity Change (Log2) – – – -0.13 -0.02 -0.22 -0.42 -0.19 -0.36 -0.47 -0.42 -0.56 0.25 0.24 0.17 0.97 1.21 1.26 0.24 0.31 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0366 1.6453 1.7471 1.9752 1.8405 1.8423 0.4898 0.4389 0.0819 -0.0678 -0.3424 0.423 1.9373 1.6588 1.7283 1.4114 1.526 1.4964
Actin 0.0261 0.0005 0.0026 0.0319 0.0162 0.0084 0.0528 0.0004 0.0262 0.0419 0.0028 0.008 0.0137 0.0018 0.0271 0.0001 0.0305 0.0055
Bud 0.0004 0.0005 0.0057 0.0002 0.0005 0.0002 0.0009 0.0001 0.0003 0.0002 0.0022 0.0001 0.0084 0.0021 0.0037 0 0.0005 0.0004
Bud Neck 0.0095 0.0012 0.0013 0.0014 0.0026 0.0124 0.0013 0.0009 0.0016 0.0011 0.0016 0.0022 0.0064 0.0012 0.0017 0.0008 0.0016 0.0038
Bud Periphery 0.0005 0.0005 0.0088 0.0003 0.0008 0.0006 0.0013 0.0001 0.0004 0.0001 0.0041 0.0001 0.0043 0.0026 0.0018 0 0.0016 0.0005
Bud Site 0.0065 0.0045 0.0028 0.0011 0.002 0.0005 0.0041 0.0017 0.003 0.0004 0.0007 0.0001 0.0044 0.0203 0.0056 0.0001 0.0006 0.0002
Cell Periphery 0.0003 0.0002 0.0003 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0 0.0002 0 0.0003 0.0002 0.0004 0 0.0003 0
Cytoplasm 0.0566 0.1147 0.0545 0.0704 0.1033 0.0611 0.0689 0.1127 0.0374 0.0444 0.0313 0.0591 0.0565 0.0811 0.0571 0.0439 0.03 0.0589
Cytoplasmic Foci 0.0131 0.0066 0.0099 0.0101 0.0209 0.0056 0.0307 0.0092 0.0203 0.014 0.0185 0.0059 0.0192 0.0068 0.0129 0.0026 0.0031 0.0059
Eisosomes 0.0003 0.0001 0.0001 0.0003 0.0002 0.0002 0.0003 0.0002 0.0004 0.0001 0 0.0001 0.0003 0.0001 0.0002 0 0.0003 0.0001
Endoplasmic Reticulum 0.0029 0.0029 0.0031 0.0025 0.0051 0.0026 0.0102 0.004 0.0014 0.0014 0.0006 0.0015 0.0043 0.0014 0.002 0.0007 0.0007 0.0009
Endosome 0.0088 0.0033 0.0061 0.0065 0.0186 0.006 0.0262 0.0012 0.0109 0.0022 0.0056 0.0041 0.0195 0.003 0.0053 0.0023 0.0015 0.0038
Golgi 0.0035 0.0001 0.0014 0.0021 0.0085 0.0027 0.0078 0.0001 0.0034 0.0012 0.0037 0.0013 0.0054 0.0005 0.004 0.0001 0.0015 0.0005
Lipid Particles 0.006 0.0007 0.0015 0.0046 0.014 0.0063 0.0057 0.0016 0.0043 0.0013 0.013 0.0077 0.0139 0.0011 0.0029 0.0001 0.002 0.0009
Mitochondria 0.0016 0.0006 0.0038 0.001 0.0147 0.0068 0.0039 0.0005 0.0025 0.0007 0.0006 0.0006 0.0035 0.0029 0.0022 0.0002 0.0287 0.0013
None 0.3028 0.3264 0.3076 0.3118 0.2248 0.1602 0.2102 0.6249 0.2516 0.0852 0.1969 0.2363 0.138 0.2237 0.1775 0.1315 0.2427 0.1577
Nuclear Periphery 0.0113 0.0112 0.0115 0.0163 0.0378 0.0177 0.0174 0.0033 0.0055 0.0242 0.0048 0.0073 0.0144 0.0094 0.0086 0.0037 0.0094 0.0063
Nucleolus 0.0119 0.0156 0.0123 0.0092 0.0355 0.0308 0.013 0.0073 0.0132 0.0069 0.0242 0.0194 0.0201 0.0203 0.0147 0.0312 0.0549 0.0308
Nucleus 0.4853 0.4324 0.4947 0.4208 0.4017 0.5797 0.4608 0.2037 0.545 0.6318 0.5736 0.5397 0.5414 0.5467 0.5726 0.7316 0.5136 0.6107
Peroxisomes 0.0051 0.0002 0.0027 0.0004 0.0259 0.0012 0.0127 0.0001 0.0056 0.0044 0.0245 0.002 0.0115 0.0075 0.0067 0.0001 0.0035 0.0009
Punctate Nuclear 0.0446 0.0745 0.0668 0.1078 0.0628 0.0953 0.0665 0.0264 0.0648 0.1372 0.0894 0.1037 0.1112 0.0603 0.0915 0.0503 0.0702 0.1096
Vacuole 0.0018 0.0031 0.0018 0.001 0.002 0.0011 0.0034 0.0012 0.0016 0.0008 0.0015 0.0004 0.0021 0.0052 0.0014 0.0005 0.0019 0.0008
Vacuole Periphery 0.001 0.0004 0.0007 0.0005 0.0019 0.0007 0.0018 0.0002 0.0003 0.0004 0.0003 0.0002 0.0011 0.0021 0.0004 0.0002 0.001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.1751 10.6982 6.8801 11.9344 12.9303 3.987 11.5381 13.1413 9.9084 12.8523
Translational Efficiency 2.371 2.2435 2.7314 1.5659 1.4 5.1261 2.0504 1.54 2.0042 1.6277

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
126 1219 1780 1265 553 906 340 12 679 2125 2120 1277

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 661.97 604.56 770.00 850.56 658.83 681.66 942.25 907.23 659.41 637.43 797.62 851.09
Standard Deviation 93.92 109.13 101.68 160.35 88.38 98.81 128.47 125.05 89.45 111.58 123.78 160.14
Intensity Change Log 2 — -0.130880 0.218093 0.361647 — 0.049146 0.516204 0.461562 — -0.038275 0.374481 0.412351

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000139 0.000388 0.000223 0.001062 0.000085 0.000638 0.000612 0.000213 0.000095 0.000495 0.000286 0.001054
Bud Neck 0.001133 0.028885 0.006220 0.024882 0.001725 0.029626 0.007226 0.004537 0.001615 0.029201 0.006381 0.024691
Bud Site 0.000519 0.010280 0.008569 0.055388 0.000846 0.010431 0.011071 0.008301 0.000786 0.010344 0.008970 0.054945
Cell Periphery 0.000169 0.000282 0.000207 0.000299 0.000179 0.000256 0.000203 0.000135 0.000177 0.000271 0.000206 0.000297
Cytoplasm 0.296462* 0.362185* 0.350617* 0.333675* 0.408075* 0.295853* 0.308768* 0.350462* 0.387364* 0.333904* 0.343905* 0.333833*
Cytoplasmic Foci 0.070597 0.201847* 0.013543 0.013566 0.087559 0.265888* 0.017135 0.039341 0.084412 0.229151* 0.014119 0.013808
Eisosomes 0.000069 0.000151 0.000041 0.000040 0.000063 0.000204 0.000074 0.000112 0.000064 0.000173 0.000046 0.000040
Endoplasmic Reticulum 0.000866 0.001788 0.001356 0.003233 0.000856 0.001294 0.004254 0.001439 0.000858 0.001577 0.001820 0.003216
Endosome 0.001715 0.023185 0.001618 0.006384 0.002975 0.021508 0.004012 0.008963 0.002741 0.022470 0.002002 0.006409
Golgi 0.001391 0.004363 0.000123 0.016362 0.000210 0.006936 0.000158 0.000083 0.000429 0.005460 0.000129 0.016209
Lipid Particles 0.003956 0.020580 0.001225 0.001050 0.003953 0.017610 0.002615 0.001478 0.003954 0.019314 0.001448 0.001054
Mitochondria 0.000935 0.013827 0.000895 0.003396 0.000726 0.025801 0.001407 0.000435 0.000765 0.018932 0.000977 0.003369
Mitotic Spindle 0.000366 0.005427 0.009979 0.042179 0.000145 0.007644 0.011215 0.000449 0.000186 0.006372 0.010177 0.041787
None 0.017577 0.025846 0.012700 0.007807 0.012361 0.005542 0.005649 0.001441 0.013329 0.017189 0.011569 0.007748
Nuclear Periphery 0.000514 0.001600 0.001226 0.001909 0.000768 0.001156 0.001460 0.000752 0.000721 0.001411 0.001264 0.001898
Nuclear Periphery Foci 0.000486 0.001522 0.000380 0.001814 0.000585 0.001984 0.001707 0.000377 0.000566 0.001719 0.000593 0.001800
Nucleolus 0.030772 0.012778 0.006532 0.007068 0.004868 0.009082 0.012869 0.001018 0.009675 0.011202 0.007548 0.007012
Nucleus 0.564349* 0.219135* 0.560742* 0.415878* 0.458290* 0.219922* 0.521229* 0.301991* 0.477971* 0.219471* 0.554405* 0.414808*
Peroxisomes 0.001919 0.013375 0.000421 0.000959 0.000946 0.031308 0.000752 0.000321 0.001126 0.021021 0.000474 0.000953
Vacuole 0.005846 0.049691 0.023175 0.057203 0.014534 0.045121 0.087318 0.277498* 0.012922 0.047743 0.033462 0.059273
Vacuole Periphery 0.000220 0.002864 0.000207 0.005845 0.000251 0.002197 0.000265 0.000654 0.000245 0.002580 0.000216 0.005796

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.83 -3.94 -7.74 -4.72 -6.21 NaN NaN NaN NaN NaN -5.38 -10.25 -8.59 -3.22 -5.23
Bud Neck -12.23 -11.63 -11.79 3.85 -6.78 NaN NaN NaN NaN NaN -16.35 -12.05 -11.44 4.84 -6.41
Bud Site -7.93 -5.52 -8.33 -4.68 -5.45 NaN NaN NaN NaN NaN -10.68 -6.04 -8.25 -4.83 -5.48
Cell Periphery -2.39 -0.85 -1.19 1.74 -0.46 NaN NaN NaN NaN NaN -3.00 -0.77 -0.95 2.17 -0.31
Cytoplasm -2.44 -0.52 0.88 8.05 3.61 NaN NaN NaN NaN NaN 4.13 6.81 8.37 6.33 2.61
Cytoplasmic Foci -10.99 6.09 6.48 32.21 3.38 NaN NaN NaN NaN NaN -20.99 14.95 15.68 45.62 3.45
Eisosomes -6.72 3.34 2.07 11.65 -4.15 NaN NaN NaN NaN NaN -14.66 3.27 2.37 17.91 -1.46
Endoplasmic Reticulum -3.21 -3.75 -10.14 -9.28 -9.19 NaN NaN NaN NaN NaN -5.28 -10.50 -13.10 -10.53 -6.55
Endosome -12.68 0.20 -1.29 13.13 -3.22 NaN NaN NaN NaN NaN -16.05 1.65 -0.26 16.35 -2.40
Golgi -1.96 1.01 -1.74 -0.34 -3.48 NaN NaN NaN NaN NaN -9.04 1.23 -3.18 0.41 -3.47
Lipid Particles -9.13 4.41 4.58 11.65 0.82 NaN NaN NaN NaN NaN -13.26 7.97 9.17 16.32 2.24
Mitochondria -10.55 0.19 -4.26 9.40 -5.21 NaN NaN NaN NaN NaN -11.99 -1.49 -5.87 10.86 -5.04
Mitotic Spindle -4.60 -6.35 -10.01 -8.05 -6.24 NaN NaN NaN NaN NaN -6.52 -7.16 -10.08 -7.88 -6.25
None -1.72 2.13 3.30 6.97 3.23 NaN NaN NaN NaN NaN -1.75 2.25 4.51 6.19 2.85
Nuclear Periphery -4.31 -12.38 -16.72 -6.58 -10.98 NaN NaN NaN NaN NaN -4.01 -11.84 -15.72 -9.36 -10.58
Nuclear Periphery Foci -4.41 0.36 -3.05 -0.07 -3.60 NaN NaN NaN NaN NaN -2.67 -2.21 -3.32 0.17 -2.68
Nucleolus 2.23 2.94 3.24 6.21 2.22 NaN NaN NaN NaN NaN -0.45 1.45 2.95 6.27 2.67
Nucleus 11.95 -1.26 3.39 -21.06 11.58 NaN NaN NaN NaN NaN 19.26 -8.08 1.13 -22.72 11.49
Peroxisomes -7.67 1.51 1.46 13.50 -0.33 NaN NaN NaN NaN NaN -15.67 2.92 2.61 16.56 -0.10
Vacuole -14.98 -10.82 -22.06 -14.05 -17.73 NaN NaN NaN NaN NaN -14.21 -11.28 -20.84 -15.08 -15.00
Vacuole Periphery -8.55 0.41 -3.00 1.61 -3.05 NaN NaN NaN NaN NaN -11.43 1.70 -2.97 1.26 -3.04
Standard name
Human Ortholog
Description Component of both the Rpd3S and Rpd3L histone deacetylase complexes; negative regulator of meiosis; required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p; UME1 has a paralog, WTM2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (28%), cytoplasm (26%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ume1

Ume1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ume1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available