Standard name
Human Ortholog
Description Karyopherin responsible for the nuclear import of Rpf1p; Rpf1p is a ribosome maturation factor
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.05
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.1 0.11 0.13 0.08 0.13 0.09 0.09 – 0.09 0.1 0.07 0.18 0.27 0.32 0.28 0.36 0.23 0 0.05 0.05 0.1 0.07 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.1 0.15 – 0.35 0.35 0.35 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.69 0.49 0.47 0.4 0.39 0.48 0.5 0.49 – 0.47 0.57 0.52 0.42 0.4 0.25 0.58 0.5 0.38 0.86 0.75 0.68 0.57 0.53 0.44
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.5 0.62 0.54 0.58 0.59 0.42 0.4 0.34 – 0.25 0.18 0.28 0.57 0.57 0.62 0.28 0.3 0.57 0.06 0.08 0.14 0.18 0.27 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 1 0 1 0 1 1 2 2 – 7 5 5 0 0 0 0 0 0 9 11 14 13 18 17
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Bud Site 0 0 0 0 0 0 2 3 – 6 6 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 10 25 54 48 37 53 48 60 – 57 43 31 38 74 106 12 23 9 9 14 26 20 24 20
Endoplasmic Reticulum 0 0 2 1 0 0 0 0 – 0 0 0 3 6 4 0 2 2 0 0 3 2 3 4
Endosome 1 0 2 5 15 3 3 7 – 0 2 4 0 0 0 1 1 0 3 3 6 4 8 11
Golgi 1 0 1 0 1 0 0 0 – 0 0 0 0 0 1 0 0 0 1 0 3 0 0 0
Mitochondria 0 9 5 6 11 37 49 104 – 211 154 165 0 0 0 0 0 1 1 7 2 0 2 4
Nucleus 317 116 229 151 176 203 257 338 – 286 248 245 91 109 82 25 32 15 426 206 355 124 195 161
Nuclear Periphery 0 0 1 1 3 6 2 6 – 12 4 13 0 0 0 0 0 0 3 0 15 2 6 5
Nucleolus 0 0 0 0 1 0 5 34 – 12 9 10 0 0 0 0 0 0 5 2 8 1 2 5
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 1 1 0 0 0 0 0 0 3 1 5 3 4 4
Vac/Vac Membrane 232 147 264 219 266 176 208 235 – 151 77 133 123 157 206 12 19 23 28 21 72 39 99 124
Unique Cell Count 461 239 486 376 453 421 515 691 604 438 471 215 274 332 43 64 40 494 274 525 218 372 369
Labelled Cell Count 562 297 559 432 511 479 576 789 742 549 611 255 346 399 50 77 50 494 274 525 218 372 369


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.1 8.1 8.1 8.1 7.5 6.8 6.8 6.6 – 5.8 5.9 6.1 9.6 8.4 9.7 7.2 7.8 10.5 9.9 10.4 11.0
Std Deviation (1e-4) 1.3 1.6 1.4 1.6 1.6 1.5 1.8 1.5 – 1.4 1.6 1.4 1.4 1.1 1.4 1.7 1.9 3.1 1.4 1.5 1.9
Intensity Change (Log2) – – – 0.0 -0.12 -0.26 -0.26 -0.29 – -0.49 -0.47 -0.42 0.24 0.05 0.25 -0.17 -0.06 0.37 0.28 0.35 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 -1.5 0.7 -0.9 -1.4 – -0.9 -0.6 -2.5 2.4 5.6 7.4 3.2 5.4 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 3.3 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.7 5.5 5.9 8.2 – 14.0 13.7 13.8 0 0 0 0 0 0
Nucleus -2.0 -2.6 0.3 0.9 0.6 – 0.1 2.9 1.5 -1.2 -2.0 -6.5 1.4 0.4 -1.2
Nuclear Periphery 0 0 0 0 0 – 2.7 0 3.3 0 0 0 0 0 0
Nucleolus 0 0 0 0 5.0 – 3.1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 1.2 1.4 -3.8 -4.4 -6.9 – -9.9 -11.6 -8.2 0.7 0.8 2.2 -3.3 -3.7 0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6923 5.9137 5.3435 5.0129 4.1496 5.1886 5.6813 7.5416 6.6189 5.858 6.4514 6.4277 5.5052 7.6961 6.1024 5.8783 5.7368 6.0234
Actin 0.0248 0.0001 0.0037 0 0.0003 0.0002 0.0263 0.0034 0.0061 0.005 0.002 0.0087 0.0063 0.0006 0.0006 0.0001 0.0083 0.009
Bud 0.0002 0 0.0001 0 0.0001 0 0.0006 0.0003 0.0002 0.0004 0.0001 0.0002 0.0001 0.0001 0.0003 0 0.0003 0.0001
Bud Neck 0.001 0.0001 0.0003 0.0003 0.0005 0.0011 0.0016 0.0015 0.0009 0.0009 0.0002 0.0014 0.0003 0.0006 0.0004 0.0003 0.0019 0.0014
Bud Periphery 0.0003 0 0.0001 0 0.0001 0 0.0012 0.0004 0.0003 0.0011 0.0002 0.0004 0.0001 0.0001 0.0004 0 0.0008 0.0003
Bud Site 0.0006 0.0001 0.0009 0 0.0002 0 0.0018 0.0176 0.0011 0.0007 0.0008 0.0001 0.0006 0.0027 0.0006 0.0001 0.0006 0.0002
Cell Periphery 0.0001 0 0 0 0 0 0.0003 0.0006 0.0001 0.0001 0 0 0 0.0002 0.0001 0 0.0004 0.0001
Cytoplasm 0.0055 0.0182 0.0065 0.0033 0.0216 0.0078 0.0198 0.0038 0.0147 0.0144 0.0011 0.0051 0.0569 0.0187 0.0313 0.0102 0.0208 0.0063
Cytoplasmic Foci 0.0093 0.0037 0.0032 0 0.0006 0.0005 0.0197 0.0017 0.0135 0.0026 0.0068 0.001 0.0243 0.0008 0.0035 0 0.0066 0.002
Eisosomes 0.0002 0 0 0 0 0 0.0002 0.0002 0.0001 0.0001 0 0.0001 0.0001 0 0 0 0 0.0001
Endoplasmic Reticulum 0.002 0.0005 0.0009 0.0008 0.0034 0.0008 0.0094 0.0006 0.0034 0.0009 0.0001 0.0019 0.002 0.0006 0.0037 0.0003 0.0019 0.0011
Endosome 0.0117 0.005 0.0017 0.0003 0.0023 0.0017 0.0274 0.0033 0.0115 0.0085 0.0026 0.0023 0.0131 0.0008 0.011 0.0001 0.0173 0.0131
Golgi 0.0045 0.0004 0.0005 0 0.0001 0.0002 0.0065 0.0016 0.0049 0.005 0.0017 0.0013 0.0043 0.0007 0.0021 0 0.0049 0.0094
Lipid Particles 0.0176 0.0013 0.0014 0 0.0001 0.0007 0.0174 0.0087 0.0077 0.0061 0.0044 0.0024 0.0262 0.0021 0.0012 0 0.0034 0.0047
Mitochondria 0.0018 0.0002 0.0013 0.0001 0.0007 0.0002 0.0107 0.0053 0.0096 0.0497 0.0003 0.0008 0.0205 0.009 0.0021 0.0002 0.0064 0.0214
None 0.0019 0.0001 0.0005 0 0.0022 0.0003 0.0116 0.01 0.0034 0.0003 0.0005 0.0004 0.007 0.0001 0.0009 0.0001 0.0025 0.0002
Nuclear Periphery 0.0298 0.0186 0.0364 0.0296 0.0346 0.0171 0.0795 0.0187 0.0356 0.0271 0.0042 0.0196 0.032 0.0345 0.0412 0.0204 0.0192 0.0173
Nucleolus 0.0018 0.0008 0.0009 0.0007 0.0032 0.0018 0.0035 0.0019 0.0006 0.0009 0.003 0.0015 0.0011 0.0009 0.0012 0.0006 0.0021 0.0015
Nucleus 0.864 0.9495 0.9363 0.9639 0.924 0.9621 0.7214 0.9107 0.8767 0.8666 0.924 0.9479 0.7746 0.9207 0.8927 0.9663 0.883 0.9059
Peroxisomes 0.0066 0.0002 0.003 0 0 0.0001 0.0076 0.0061 0.003 0.0056 0.0035 0.0019 0.0185 0.0048 0.0004 0 0.0036 0.0008
Punctate Nuclear 0.015 0.0006 0.0012 0.0003 0.0039 0.0046 0.0271 0.0023 0.0056 0.0017 0.044 0.0014 0.0097 0.0009 0.003 0.001 0.0071 0.0021
Vacuole 0.0008 0.0004 0.0005 0.0003 0.0016 0.0005 0.0042 0.0012 0.0008 0.0013 0.0001 0.0013 0.0014 0.0008 0.0024 0.0002 0.0075 0.0016
Vacuole Periphery 0.0006 0.0002 0.0005 0.0002 0.0005 0.0002 0.0022 0.0003 0.0004 0.0009 0.0001 0.0004 0.0012 0.0003 0.0009 0.0001 0.0015 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.3743 15.5961 16.5329 28.7413 20.312 17.7927 23.6539 27.2596 24.2068 32.0855
Translational Efficiency 0.6144 0.9808 1.0207 0.5613 0.7406 1.0972 0.737 0.5545 0.6418 0.6064

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
450 1344 872 128 216 1338 2198 1762 666 2682 3070 1890

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 977.94 978.48 1058.28 1158.32 866.69 989.94 1038.51 1119.34 941.86 984.20 1044.13 1121.98
Standard Deviation 150.29 161.98 210.72 169.03 112.79 134.15 150.77 172.94 148.66 148.86 170.19 172.95
Intensity Change Log 2 — 0.000796 0.113904 0.244216 — 0.191825 0.260927 0.369060 — 0.093707 0.184851 0.304221

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002576 0.000409 0.000676 0.000809 0.000617 0.000233 0.000257 0.000868 0.001941 0.000322 0.000376 0.000864
Bud Neck 0.007973 0.002649 0.006797 0.006391 0.002532 0.002876 0.013178 0.011576 0.006208 0.002762 0.011365 0.011225
Bud Site 0.014702 0.000509 0.001141 0.005475 0.000828 0.000553 0.002018 0.008865 0.010203 0.000531 0.001769 0.008636
Cell Periphery 0.000256 0.000076 0.000126 0.000171 0.000196 0.000057 0.000163 0.000132 0.000236 0.000067 0.000153 0.000135
Cytoplasm 0.107949* 0.041968 0.031789 0.134904* 0.037086 0.028448 0.037542 0.080777 0.084966 0.035223 0.035908 0.084442
Cytoplasmic Foci 0.020109 0.000976 0.000174 0.008726 0.002162 0.002296 0.000357 0.000631 0.014288 0.001635 0.000305 0.001179
Eisosomes 0.000059 0.000012 0.000011 0.000058 0.000027 0.000014 0.000011 0.000015 0.000049 0.000013 0.000011 0.000018
Endoplasmic Reticulum 0.008513 0.010403 0.004443 0.006380 0.016875 0.007786 0.002906 0.006527 0.011225 0.009097 0.003342 0.006517
Endosome 0.019533 0.000603 0.000281 0.014758 0.001113 0.001265 0.000721 0.001015 0.013559 0.000933 0.000596 0.001946
Golgi 0.017750 0.000383 0.000084 0.003900 0.000090 0.000817 0.000049 0.000435 0.012023 0.000600 0.000059 0.000670
Lipid Particles 0.005583 0.000912 0.000141 0.001925 0.001009 0.001051 0.000174 0.000265 0.004099 0.000981 0.000165 0.000377
Mitochondria 0.016971 0.001418 0.001154 0.001528 0.000780 0.001631 0.001316 0.002400 0.011720 0.001524 0.001270 0.002341
Mitotic Spindle 0.021746 0.002100 0.002893 0.118214* 0.000034 0.003988 0.004820 0.011809 0.014704 0.003042 0.004272 0.019015
None 0.006324 0.004044 0.006055 0.007017 0.003696 0.001847 0.006189 0.007978 0.005472 0.002948 0.006151 0.007913
Nuclear Periphery 0.002900 0.004085 0.001092 0.005325 0.003042 0.004854 0.001570 0.002930 0.002946 0.004468 0.001434 0.003092
Nuclear Periphery Foci 0.002048 0.001570 0.000487 0.009098 0.003533 0.001849 0.000087 0.000968 0.002530 0.001710 0.000201 0.001519
Nucleolus 0.003389 0.002980 0.001161 0.001456 0.005737 0.002110 0.000673 0.001087 0.004150 0.002546 0.000812 0.001112
Nucleus 0.677099* 0.920335* 0.937874* 0.556588* 0.914448* 0.932254* 0.924538* 0.845225* 0.754077* 0.926281* 0.928326* 0.825677*
Peroxisomes 0.003537 0.000568 0.000082 0.000264 0.000322 0.000764 0.000076 0.000213 0.002495 0.000666 0.000078 0.000216
Vacuole 0.051894 0.003731 0.003359 0.107375 0.005532 0.004991 0.003196 0.015449 0.036858 0.004360 0.003243 0.021675
Vacuole Periphery 0.009089 0.000267 0.000183 0.009637 0.000339 0.000316 0.000159 0.000834 0.006251 0.000291 0.000166 0.001430

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.29 1.63 0.58 -4.90 -2.94 3.01 2.21 -1.31 -3.83 -3.23 2.70 2.33 1.00 -3.73 -2.87
Bud Neck 5.10 -0.52 -1.63 -4.39 -1.46 -1.50 -10.97 -13.00 -10.95 0.03 4.21 -6.38 -7.49 -12.83 -1.26
Bud Site 3.89 3.55 0.52 -6.88 -6.16 0.52 -3.25 -5.25 -5.33 -3.96 3.88 3.06 0.01 -5.94 -4.62
Cell Periphery 5.80 3.15 -0.48 -6.90 -3.94 3.98 1.52 2.19 -7.98 0.32 7.23 2.43 3.68 -8.20 0.07
Cytoplasm 5.92 7.73 0.01 -3.84 -5.02 1.24 0.50 -5.32 -11.60 -11.30 6.36 7.12 0.37 -10.98 -12.85
Cytoplasmic Foci 4.64 5.12 2.95 -3.66 -4.98 -0.33 2.84 2.28 3.48 -2.35 4.23 5.46 4.97 2.79 -4.33
Eisosomes 12.62 12.31 -2.70 -7.99 -7.88 3.25 3.61 1.50 -4.71 -7.33 12.37 12.48 7.95 -9.70 -10.35
Endoplasmic Reticulum -0.10 7.12 0.26 0.36 -4.94 3.77 5.62 4.18 1.46 -9.23 3.33 9.13 5.49 4.20 -9.80
Endosome 4.09 4.24 2.21 -2.98 -3.26 -0.48 1.64 0.52 0.96 -1.52 3.93 4.23 3.77 -1.26 -3.12
Golgi 2.79 2.90 2.65 -0.87 -1.84 -1.51 2.00 -1.30 1.19 -1.69 2.60 2.91 2.80 1.18 -1.99
Lipid Particles 1.51 2.03 0.66 -2.59 -6.68 -0.21 3.67 2.31 2.49 -5.62 1.46 2.30 1.87 1.54 -8.53
Mitochondria 2.98 3.10 3.06 0.02 -0.58 -2.50 -6.64 -9.76 -2.19 -5.27 2.78 2.88 2.27 -2.47 -5.80
Mitotic Spindle 3.49 3.32 -2.42 -3.45 -3.42 -3.24 -4.61 -7.61 -3.95 -4.00 2.77 2.41 -1.40 -6.06 -5.53
None 1.36 0.47 -0.29 -1.41 -0.69 2.21 -1.87 -3.73 -10.12 -2.37 2.70 -0.43 -2.50 -7.46 -2.76
Nuclear Periphery -1.38 3.81 -1.71 -0.67 -6.84 -2.55 1.74 -2.67 0.75 -12.34 -2.59 3.52 -2.78 0.27 -14.60
Nuclear Periphery Foci 0.47 3.69 -1.76 -2.08 -3.61 1.21 2.87 1.89 1.30 -6.37 1.14 4.81 2.13 1.28 -6.77
Nucleolus -0.21 1.42 1.38 2.81 -0.10 2.30 3.63 3.28 2.31 -2.90 0.95 3.03 2.69 3.68 -2.30
Nucleus -13.59 -14.89 3.48 11.41 12.13 -1.38 -0.96 7.93 14.33 15.51 -12.87 -13.43 -3.65 17.57 19.16
Peroxisomes 2.06 3.28 2.83 0.90 -2.73 -1.72 4.65 2.99 2.47 -1.69 1.54 3.56 3.32 2.74 -1.95
Vacuole 10.96 10.70 -4.28 -8.95 -8.86 -0.11 0.42 -11.21 -12.49 -13.11 10.44 10.38 3.68 -14.89 -14.70
Vacuole Periphery 3.05 3.13 1.66 -1.13 -1.21 0.24 2.07 -0.46 -0.61 -1.56 3.05 3.22 2.74 -1.08 -1.86
Standard name
Human Ortholog
Description Karyopherin responsible for the nuclear import of Rpf1p; Rpf1p is a ribosome maturation factor
Localization
Cell Percentages nucleus (24%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Kap120

Kap120


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kap120-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available