Standard name
Description Mitochondrial aspartyl-tRNA synthetase; required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.06 0.05 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.08 0 0 0 0 0 0 0 0 0.1 0.1 0 0.06 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.05 0.13 0.24 0.12 0.15 0.07 0.1 0 0 0 0 0.2 0.16 0.28 0.18 0.2 0.22 0.1 0.13 0.13 0.12 0.1 0.06
Golgi 0 0 0 0 0.07 0 0 0 0 0 0 0.14 0.14 0.12 0.11 0.1 0.09 0 0 0 0 0 0
Mitochondria 0.91 0.65 0.33 0.67 0.52 0.75 0.74 0.9 0.85 0.96 0.92 0.54 0.43 0.25 0.13 0.16 0.13 0.67 0.65 0.58 0.78 0.7 0.65
Nucleus 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0.07 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.1 0 0.13 0.09 0 0 0 0 0 0 0.06 0.08 0.24 0.23 0.23 0.23 0.06 0.06 0.08 0 0 0
SpindlePole 0.1 0.1 0.33 0.13 0.16 0.07 0.08 0.06 0.07 0 0 0.12 0.08 0.24 0.3 0.21 0.25 0 0 0.05 0 0 0
Vac/Vac Membrane 0 0.2 0.24 0 0.14 0.09 0.13 0.1 0.07 0 0.06 0.08 0.07 0.11 0.2 0.22 0.2 0 0 0.06 0 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 6 2 0 0 0 1 1 0 4 8 0 2 2 1 3 3 2 0 3 7
Bud 2 0 1 8 7 7 11 9 12 9 10 1 5 7 6 10 6 0 1 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 0 0 0 0 1 2
Bud Site 0 0 0 0 0 2 2 5 3 6 3 0 1 0 2 1 4
Cell Periphery 0 5 1 5 7 2 5 2 5 3 1 3 7 5 12 11 7 0 0 0 0 0 1
Cytoplasm 3 8 1 15 12 8 15 5 6 1 5 3 10 16 7 15 15 0 1 0 0 0 5
Endoplasmic Reticulum 1 0 0 2 4 0 2 0 5 1 3 1 3 1 2 7 7 0 0 1 0 0 1
Endosome 6 17 11 22 51 16 37 9 11 0 5 17 16 46 37 46 40 13 18 12 6 18 13
Golgi 0 2 1 8 24 0 3 1 0 0 3 12 14 19 23 24 17 4 6 1 1 4 9
Mitochondria 107 85 15 125 180 177 276 252 382 247 297 45 44 41 28 37 24 84 92 57 38 132 152
Nucleus 0 1 0 1 2 12 15 4 6 3 8 1 2 0 2 7 5 0 0 0 0 0 1
Nuclear Periphery 1 1 0 0 2 3 11 11 33 18 15 1 0 0 1 1 0 1 0 0 0 0 1
Nucleolus 7 1 1 0 2 12 11 8 18 11 11 1 0 1 2 6 4 1 0 0 0 0 0
Peroxisomes 4 13 2 24 30 7 16 3 3 2 2 5 8 40 48 53 42 7 7 7 0 6 8
SpindlePole 12 13 15 25 54 17 29 17 31 7 13 10 8 39 62 50 45 3 3 5 0 1 4
Vac/Vac Membrane 5 26 11 4 49 21 48 29 33 11 18 7 7 18 43 51 37 0 3 6 1 13 20
Unique Cell Count 117 131 46 187 343 236 374 281 451 258 324 83 103 165 210 234 183 126 142 99 50 190 236
Labelled Cell Count 148 174 59 245 426 284 481 355 549 320 394 111 133 233 279 323 255 126 142 99 50 190 236


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.9 6.1 6.3 6.9 5.5 5.9 5.3 5.4 5.0 5.3 6.4 6.5 6.4 7.6 7.9 7.5 6.1 6.2
Std Deviation (1e-4) 0.6 0.9 1.0 1.7 2.3 1.3 1.3 0.9 1.1 1.2 1.2 1.1 0.9 1.1 1.5 2.1 2.0 1.2 1.2
Intensity Change (Log2) 0.06 0.18 -0.15 -0.04 -0.21 -0.17 -0.27 -0.19 0.08 0.11 0.08 0.33 0.39 0.31 0.01 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -2.1 -1.6 0 -2.8 0 0 0 0 -0.5 -1.2 0.5 -1.0 -0.7 -0.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.2 2.5 5.6 5.7 9.3 8.4 11.4 10.3 2.4 1.2 -1.1 -3.2 -2.7 -3.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1.6 0 0 0 0 0 0 0 0 0 3.0 2.9 2.9 2.9
SpindlePole -3.1 -2.8 -5.0 0 0 0 0 0 -2.8 -3.9 -1.2 -0.4 -1.7 -1.1
Vacuole 0 -1.7 -2.9 -2.0 -2.6 0 0 0 -2.4 -3.0 -2.3 -0.5 -0.3 -0.6


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available