Standard name | |
---|---|
Human Ortholog | |
Description | Cytoplasmic class E vacuolar protein sorting (VPS) factor; coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 30 | 83 | 39 | 46 | 35 | 100 | 105 | 68 | – | 63 | 84 | 42 | 34 | 29 | 50 | 21 | 15 | 1 | 5 | 6 | 8 | 15 | 20 |
Bud | 2 | 2 | 15 | 5 | 9 | 12 | 32 | 32 | 31 | – | 28 | 32 | 8 | 9 | 14 | 4 | 3 | 2 | 0 | 1 | 1 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 14 | 47 | 7 | 20 | 20 | 26 | 37 | 28 | – | 36 | 32 | 25 | 34 | 79 | 86 | 53 | 42 | 4 | 5 | 15 | 2 | 0 | 0 |
Endoplasmic Reticulum | 1 | 22 | 46 | 8 | 8 | 8 | 31 | 17 | 25 | – | 23 | 34 | 34 | 38 | 52 | 16 | 4 | 8 | 0 | 0 | 6 | 4 | 2 | 4 |
Endosome | 53 | 71 | 97 | 127 | 192 | 214 | 277 | 197 | 137 | – | 167 | 123 | 221 | 277 | 259 | 55 | 43 | 32 | 71 | 123 | 258 | 129 | 356 | 359 |
Golgi | 5 | 18 | 10 | 19 | 21 | 12 | 31 | 34 | 8 | – | 17 | 20 | 31 | 67 | 43 | 45 | 35 | 20 | 7 | 9 | 12 | 6 | 33 | 44 |
Mitochondria | 11 | 9 | 1 | 19 | 9 | 31 | 36 | 12 | 91 | – | 137 | 50 | 7 | 2 | 3 | 8 | 6 | 0 | 2 | 0 | 13 | 1 | 2 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 2 | 0 | 0 | 3 | 2 | 1 | 3 | 3 | – | 4 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 5 | 4 | 1 | 8 | 23 | 21 | 16 | 8 | 11 | – | 12 | 8 | 5 | 6 | 10 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 3 | 6 | 4 | 14 | 7 | 3 | – | 7 | 3 | 12 | 7 | 5 | 0 | 0 | 1 | 2 | 2 | 3 | 1 | 10 | 13 |
SpindlePole | 2 | 1 | 28 | 32 | 71 | 38 | 94 | 95 | 23 | – | 43 | 54 | 9 | 18 | 11 | 30 | 12 | 7 | 6 | 5 | 7 | 1 | 10 | 4 |
Vac/Vac Membrane | 5 | 20 | 28 | 12 | 14 | 17 | 19 | 25 | 13 | – | 17 | 21 | 36 | 25 | 18 | 51 | 33 | 35 | 1 | 4 | 23 | 5 | 3 | 3 |
Unique Cell Count | 71 | 139 | 269 | 202 | 316 | 309 | 493 | 406 | 333 | 397 | 333 | 341 | 437 | 442 | 280 | 167 | 134 | 107 | 162 | 356 | 166 | 441 | 459 | |
Labelled Cell Count | 94 | 193 | 356 | 279 | 422 | 414 | 682 | 572 | 447 | 556 | 462 | 432 | 518 | 523 | 349 | 213 | 166 | 107 | 162 | 356 | 166 | 441 | 459 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.5 | 11.1 | 9.2 | 8.7 | 9.8 | 8.2 | 8.3 | 8.7 | 7.3 | – | 7.4 | 7.8 | 10.8 | 11.4 | 11.5 | 15.6 | 15.8 | 15.6 | 9.2 | 9.9 | 10.2 |
Std Deviation (1e-4) | 1.5 | 1.7 | 1.3 | 1.2 | 1.4 | 1.2 | 1.3 | 1.4 | 1.4 | – | 1.4 | 1.4 | 1.5 | 1.5 | 1.7 | 3.8 | 3.2 | 2.9 | 1.2 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.09 | -0.16 | -0.14 | -0.08 | -0.33 | – | -0.32 | -0.24 | 0.23 | 0.31 | 0.33 | 0.77 | 0.78 | 0.77 | 0.0 | 0.1 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.8 | -4.7 | -5.8 | -3.3 | -1.4 | -2.9 | – | -4.6 | -1.5 | -5.6 | -8.0 | -8.6 | -3.6 | -4.4 | -4.3 |
Bud | -1.7 | -1.7 | -1.0 | 0.5 | 1.2 | 1.7 | – | 0.8 | 1.8 | -2.1 | -2.5 | -1.6 | -2.7 | -1.9 | -1.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -4.7 | -4.2 | -4.1 | -5.5 | -3.2 | -3.3 | – | -3.2 | -2.8 | -3.9 | -3.9 | 0.1 | 3.6 | 3.4 | 3.2 |
Endoplasmic Reticulum | -4.4 | -6.1 | -6.0 | -4.7 | -5.6 | -3.6 | – | -4.7 | -2.5 | -2.6 | -3.3 | -2.0 | -4.2 | -4.7 | -3.1 |
Endosome | 5.8 | 6.0 | 8.0 | 5.3 | 3.2 | 1.3 | – | 1.6 | 0.2 | 7.1 | 7.1 | 5.8 | -4.3 | -2.2 | -2.5 |
Golgi | 2.5 | 1.6 | 0.1 | 1.5 | 2.4 | -0.9 | – | 0.4 | 1.3 | 2.6 | 4.8 | 3.0 | 4.8 | 5.8 | 4.0 |
Mitochondria | 4.8 | 0 | 5.1 | 4.3 | 2.4 | 9.1 | – | 10.7 | 6.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 4.2 | 4.0 | 2.6 | 0 | 2.6 | – | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1.8 | 3.9 | 0.7 | 3.1 | 4.3 | -1.5 | – | 0.2 | 2.1 | -4.0 | -3.3 | -4.5 | 0.1 | -1.1 | -1.7 |
Vacuole | -1.7 | -2.8 | -2.2 | -3.6 | -2.0 | -3.1 | – | -3.1 | -1.8 | 0.1 | -2.3 | -3.3 | 2.6 | 2.7 | 4.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.0439 | 8.9433 | 8.1927 | 7.1829 | 5.8977 | 8.4551 | 5.0178 | 7.5302 | 6.7829 | 5.1567 | 5.6819 | 6.4394 | 5.1888 | 7.6345 | 7.1246 | 6.3037 | 5.1264 | 7.0305 |
Actin | 0.0026 | 0.003 | 0.0488 | 0.0416 | 0.1706 | 0.0006 | 0.0242 | 0.0032 | 0.0046 | 0.0006 | 0.0004 | 0.004 | 0.0618 | 0.0032 | 0.0083 | 0.044 | 0.1224 | 0.0003 |
Bud | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0005 | 0 | 0.0002 | 0.0008 | 0.0005 | 0.0004 | 0.0006 | 0.0015 | 0.0001 |
Bud Neck | 0.0003 | 0.0002 | 0.0006 | 0.0012 | 0.0004 | 0.0005 | 0.0027 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0005 | 0.0015 | 0.0003 | 0.0004 | 0.0002 | 0.0006 | 0.0003 |
Bud Periphery | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0008 | 0 | 0.0001 | 0.0009 | 0.0002 | 0.0004 | 0.0007 | 0.0023 | 0 |
Bud Site | 0.0005 | 0.0005 | 0.0014 | 0.0005 | 0.0006 | 0.0002 | 0.0037 | 0.0003 | 0.0007 | 0.0007 | 0.0001 | 0.0002 | 0.0033 | 0.002 | 0.0013 | 0.0013 | 0.0015 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0242 | 0.0456 | 0.0526 | 0.0365 | 0.0092 | 0.0404 | 0.0362 | 0.0349 | 0.0363 | 0.0432 | 0.0292 | 0.0199 | 0.0289 | 0.0713 | 0.0627 | 0.0366 | 0.0076 | 0.0108 |
Cytoplasmic Foci | 0.2692 | 0.3723 | 0.2446 | 0.2679 | 0.2291 | 0.2807 | 0.2338 | 0.3495 | 0.3066 | 0.274 | 0.2815 | 0.2934 | 0.1664 | 0.2721 | 0.2502 | 0.1794 | 0.158 | 0.2881 |
Eisosomes | 0 | 0 | 0.0011 | 0.0004 | 0.0012 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0006 | 0 | 0.0001 | 0.0007 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0159 | 0.0013 | 0.0033 | 0.0027 | 0.0015 | 0.0023 | 0.0029 | 0.0006 | 0.0012 | 0.0008 | 0.0005 | 0.0007 | 0.0085 | 0.0012 | 0.0011 | 0.0023 | 0.0039 | 0.0007 |
Endosome | 0.4672 | 0.4588 | 0.4836 | 0.5496 | 0.4618 | 0.4347 | 0.5079 | 0.5122 | 0.5362 | 0.5066 | 0.5838 | 0.5053 | 0.4933 | 0.4956 | 0.5094 | 0.5428 | 0.4604 | 0.5267 |
Golgi | 0.0785 | 0.0808 | 0.1071 | 0.0755 | 0.0434 | 0.1616 | 0.1064 | 0.0723 | 0.0814 | 0.053 | 0.0928 | 0.1466 | 0.1024 | 0.0674 | 0.0904 | 0.0495 | 0.1208 | 0.1168 |
Lipid Particles | 0.068 | 0.0157 | 0.0171 | 0.0052 | 0.0268 | 0.0497 | 0.0171 | 0.0075 | 0.004 | 0.0105 | 0.0013 | 0.0077 | 0.0208 | 0.0105 | 0.0145 | 0.0127 | 0.0193 | 0.017 |
Mitochondria | 0.0017 | 0.0006 | 0.0098 | 0.0009 | 0.0062 | 0.0038 | 0.0087 | 0.0004 | 0.0098 | 0.0753 | 0.0002 | 0.0041 | 0.0218 | 0.0006 | 0.006 | 0.0325 | 0.0429 | 0.0005 |
None | 0.001 | 0.0002 | 0.0005 | 0.0009 | 0.0026 | 0.0001 | 0.0085 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0027 | 0.0022 | 0.0003 | 0.0011 | 0.0015 | 0.0001 |
Nuclear Periphery | 0.0146 | 0.0002 | 0.0005 | 0.0012 | 0.0026 | 0.0002 | 0.0101 | 0.0002 | 0.0006 | 0.002 | 0.0001 | 0.0002 | 0.0065 | 0.0005 | 0.0006 | 0.0029 | 0.0297 | 0.0004 |
Nucleolus | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0004 | 0 | 0 | 0.0005 | 0 | 0 | 0.0025 | 0.0037 | 0.0004 | 0.0003 | 0.0003 | 0.0002 |
Nucleus | 0.0009 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0025 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0548 | 0.0476 | 0.0282 | 0.067 | 0.0045 | 0.0249 |
Peroxisomes | 0.0405 | 0.013 | 0.0127 | 0.0062 | 0.0318 | 0.0209 | 0.0175 | 0.0131 | 0.0105 | 0.0078 | 0.006 | 0.0116 | 0.0065 | 0.0104 | 0.0074 | 0.0104 | 0.0112 | 0.007 |
Punctate Nuclear | 0.0036 | 0.0005 | 0.0005 | 0.0007 | 0.0084 | 0.0004 | 0.0043 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0057 | 0.0007 | 0.0007 | 0.001 | 0.0028 | 0.0005 |
Vacuole | 0.0085 | 0.0054 | 0.0123 | 0.0056 | 0.0013 | 0.0022 | 0.0089 | 0.0035 | 0.0043 | 0.012 | 0.0021 | 0.0029 | 0.0063 | 0.0077 | 0.0136 | 0.0086 | 0.0035 | 0.0036 |
Vacuole Periphery | 0.0024 | 0.0015 | 0.0024 | 0.0026 | 0.0011 | 0.0013 | 0.0031 | 0.0015 | 0.0024 | 0.0102 | 0.0015 | 0.0021 | 0.0035 | 0.0022 | 0.0037 | 0.0052 | 0.0048 | 0.0019 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.7165 | 21.0116 | 15.8802 | 27.178 | 16.0838 | 14.7909 | 20.6546 | 26.8188 | 25.2263 | 24.8766 |
Translational Efficiency | 1.0088 | 0.9836 | 1.3637 | 0.8446 | 1.19 | 1.4799 | 0.9963 | 0.7459 | 0.8785 | 0.7718 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1619 | 1181 | 606 | 1695 | 184 | 109 | 228 | 107 | 1803 | 1290 | 834 | 1802 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 788.69 | 995.73 | 1417.95 | 1383.43 | 1052.83 | 1205.68 | 1310.98 | 1517.02 | 815.65 | 1013.47 | 1388.71 | 1391.36 |
Standard Deviation | 102.38 | 150.17 | 170.77 | 203.42 | 129.79 | 190.76 | 218.14 | 298.68 | 132.38 | 164.71 | 190.97 | 212.64 |
Intensity Change Log 2 | — | 0.336296 | 0.846276 | 0.810719 | — | 0.195575 | 0.316373 | 0.526968 | — | 0.257531 | 0.567438 | 0.655380 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001730 | 0.001556 | 0.004976 | 0.009737 | 0.003745 | 0.001341 | 0.001452 | 0.005554 | 0.001936 | 0.001538 | 0.004013 | 0.009489 |
Bud Neck | 0.001345 | 0.000825 | 0.003655 | 0.002617 | 0.002081 | 0.000684 | 0.004522 | 0.001858 | 0.001420 | 0.000813 | 0.003892 | 0.002572 |
Bud Site | 0.003418 | 0.011210 | 0.037394 | 0.035156 | 0.009640 | 0.008410 | 0.038309 | 0.016916 | 0.004053 | 0.010973 | 0.037644 | 0.034073 |
Cell Periphery | 0.000031 | 0.000034 | 0.000161 | 0.000285 | 0.000139 | 0.000057 | 0.000132 | 0.000365 | 0.000042 | 0.000036 | 0.000153 | 0.000290 |
Cytoplasm | 0.004513 | 0.030570 | 0.030412 | 0.038242 | 0.007191 | 0.005984 | 0.034597 | 0.010769 | 0.004786 | 0.028492 | 0.031556 | 0.036610 |
Cytoplasmic Foci | 0.239293* | 0.317674* | 0.284003* | 0.226096* | 0.241259* | 0.286403* | 0.336873* | 0.126314 | 0.239494* | 0.315032* | 0.298457* | 0.220171* |
Eisosomes | 0.000027 | 0.000012 | 0.000015 | 0.000037 | 0.000019 | 0.000018 | 0.000012 | 0.000045 | 0.000026 | 0.000012 | 0.000014 | 0.000038 |
Endoplasmic Reticulum | 0.000071 | 0.000212 | 0.000336 | 0.000273 | 0.000280 | 0.000507 | 0.000100 | 0.002210 | 0.000092 | 0.000237 | 0.000271 | 0.000388 |
Endosome | 0.523599* | 0.443015* | 0.280684* | 0.337391* | 0.573913* | 0.456342* | 0.344906* | 0.309195* | 0.528734* | 0.444141* | 0.298241* | 0.335717* |
Golgi | 0.129157* | 0.148950* | 0.129619* | 0.233418* | 0.108680 | 0.182959* | 0.049756 | 0.343667* | 0.127067* | 0.151823* | 0.107786 | 0.239965* |
Lipid Particles | 0.012963 | 0.008523 | 0.019886 | 0.019752 | 0.005745 | 0.003051 | 0.009655 | 0.028792 | 0.012226 | 0.008060 | 0.017089 | 0.020289 |
Mitochondria | 0.004368 | 0.000642 | 0.000066 | 0.001896 | 0.000709 | 0.001127 | 0.000038 | 0.004285 | 0.003995 | 0.000683 | 0.000059 | 0.002038 |
Mitotic Spindle | 0.001797 | 0.007146 | 0.060271 | 0.015496 | 0.002737 | 0.008884 | 0.045243 | 0.001596 | 0.001893 | 0.007293 | 0.056162 | 0.014670 |
None | 0.000049 | 0.000044 | 0.000012 | 0.000021 | 0.000093 | 0.000011 | 0.000010 | 0.000023 | 0.000054 | 0.000041 | 0.000011 | 0.000021 |
Nuclear Periphery | 0.000016 | 0.000024 | 0.000354 | 0.000167 | 0.000071 | 0.000431 | 0.000238 | 0.000389 | 0.000022 | 0.000059 | 0.000322 | 0.000180 |
Nuclear Periphery Foci | 0.000357 | 0.001157 | 0.007447 | 0.001156 | 0.001620 | 0.005841 | 0.005557 | 0.002270 | 0.000486 | 0.001553 | 0.006931 | 0.001222 |
Nucleolus | 0.000110 | 0.000618 | 0.000032 | 0.000033 | 0.000100 | 0.000312 | 0.000118 | 0.000018 | 0.000109 | 0.000592 | 0.000056 | 0.000032 |
Nucleus | 0.000232 | 0.001179 | 0.000085 | 0.000056 | 0.004152 | 0.000441 | 0.000098 | 0.000022 | 0.000632 | 0.001117 | 0.000089 | 0.000054 |
Peroxisomes | 0.072809 | 0.011287 | 0.009182 | 0.019184 | 0.012025 | 0.010170 | 0.026673 | 0.008710 | 0.066606 | 0.011193 | 0.013964 | 0.018562 |
Vacuole | 0.003006 | 0.011986 | 0.120008 | 0.049664 | 0.017940 | 0.019426 | 0.097988 | 0.115942 | 0.004530 | 0.012615 | 0.113988 | 0.053600 |
Vacuole Periphery | 0.001108 | 0.003336 | 0.011399 | 0.009323 | 0.007861 | 0.007602 | 0.003725 | 0.021060 | 0.001797 | 0.003697 | 0.009301 | 0.010020 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.32 | -2.15 | -6.25 | -6.43 | -2.60 | 0.94 | 0.82 | -0.43 | -1.26 | -1.17 | 0.71 | -1.83 | -6.10 | -6.56 | -3.51 |
Bud Neck | 2.06 | -6.60 | -4.73 | -8.04 | 3.11 | 1.55 | -2.88 | 0.14 | -1.99 | 3.53 | 2.46 | -7.49 | -4.45 | -8.31 | 4.38 |
Bud Site | -5.96 | -11.26 | -15.57 | -10.58 | 0.63 | 0.24 | -5.78 | -1.35 | -1.57 | 3.56 | -5.60 | -13.29 | -15.36 | -10.77 | 1.43 |
Cell Periphery | -0.57 | -10.37 | -8.34 | -8.29 | -3.79 | 1.20 | -0.10 | -2.23 | -3.90 | -2.21 | 0.73 | -5.78 | -8.34 | -8.78 | -3.87 |
Cytoplasm | -7.29 | -7.24 | -12.23 | -2.25 | -0.99 | 0.26 | -4.18 | -0.89 | -1.08 | 3.19 | -7.24 | -8.50 | -12.22 | -2.49 | -0.63 |
Cytoplasmic Foci | -10.45 | -4.49 | 2.09 | 12.24 | 5.96 | -2.14 | -4.27 | 5.99 | 7.23 | 9.93 | -10.65 | -6.44 | 3.17 | 13.28 | 8.75 |
Eisosomes | 2.96 | 2.19 | -1.59 | -5.29 | -4.43 | 0.17 | 1.07 | -1.70 | -1.69 | -2.26 | 2.96 | 2.43 | -1.89 | -5.47 | -4.93 |
Endoplasmic Reticulum | -2.38 | -3.74 | -3.61 | -0.66 | 0.75 | -0.52 | 1.44 | -1.17 | -1.02 | -1.27 | -2.25 | -3.36 | -2.60 | -1.15 | -0.94 |
Endosome | 7.99 | 23.86 | 21.62 | 11.77 | -6.21 | 3.90 | 10.21 | 9.48 | 4.58 | 0.63 | 8.85 | 25.04 | 23.49 | 12.54 | -4.99 |
Golgi | -3.27 | 0.60 | -14.88 | -10.80 | -11.58 | -3.27 | 5.54 | -8.09 | -4.80 | -10.84 | -4.23 | 3.82 | -16.59 | -11.51 | -17.02 |
Lipid Particles | 2.46 | -2.78 | -3.47 | -6.23 | 0.11 | 1.33 | -1.70 | -2.81 | -3.17 | -2.22 | 2.53 | -2.49 | -4.25 | -6.96 | -1.26 |
Mitochondria | 4.22 | 4.97 | 2.72 | -3.27 | -5.46 | -0.41 | 1.36 | -0.93 | -0.81 | -1.11 | 4.15 | 5.05 | 2.31 | -3.14 | -5.09 |
Mitotic Spindle | -3.90 | -9.18 | -8.68 | -4.31 | 6.71 | -1.13 | -5.09 | 0.79 | 1.34 | 5.24 | -4.03 | -10.54 | -8.59 | -3.97 | 7.73 |
None | 0.29 | 2.60 | 1.99 | 1.42 | -3.62 | 1.01 | 0.96 | 0.87 | -1.09 | -0.89 | 0.63 | 2.68 | 2.15 | 1.36 | -3.26 |
Nuclear Periphery | -0.99 | -6.74 | -5.47 | -5.03 | 3.38 | -0.91 | -3.06 | -1.98 | 0.10 | -0.78 | -1.11 | -7.96 | -5.68 | -2.82 | 3.30 |
Nuclear Periphery Foci | -3.16 | -7.47 | -6.04 | -0.04 | 6.59 | -0.73 | -2.44 | -0.50 | 0.62 | 2.33 | -2.03 | -8.22 | -4.36 | 0.58 | 7.30 |
Nucleolus | -1.06 | 1.19 | 1.21 | 1.24 | 0.26 | -0.68 | -0.53 | 1.10 | 0.99 | 3.81 | -1.10 | 0.76 | 1.34 | 1.29 | 3.20 |
Nucleus | -1.42 | 1.05 | 1.33 | 1.67 | 1.95 | 1.12 | 1.18 | 1.24 | 0.99 | 2.60 | -0.73 | 1.39 | 1.59 | 1.72 | 2.78 |
Peroxisomes | 16.27* | 16.93 | 13.80 | -4.23 | -5.57 | 0.30 | -3.12 | 0.54 | 0.34 | 2.45 | 16.07 | 14.76 | 13.50 | -4.05 | -2.21 |
Vacuole | -8.29 | -20.40 | -22.23 | -16.54 | 11.42 | -0.23 | -7.96 | -5.89 | -5.84 | -0.33 | -7.02 | -22.13 | -21.74 | -17.21 | 11.37 |
Vacuole Periphery | -6.00 | -8.36 | -13.62 | -8.92 | 1.35 | 0.08 | 1.12 | -2.33 | -2.79 | -4.08 | -3.44 | -7.45 | -11.26 | -8.95 | -0.89 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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