Standard name
Human Ortholog
Description Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase; ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex, which in turn determines the shape of inner membrane cristae; phosphorylated
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.17 0.17 0.12 0.09 0.2 0.13 0.16 0.19 0.17
Bud – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0 0 0.06 0 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0.1 – 0 0 0 0.06 0.07 0.1 0.76 0.79 0.8 0.23 0.25 0.19 0.3 0.24 0.33
Mitochondria – 0.98 0.95 0.82 0.85 0.91 0.79 0.63 – 0.9 0.88 0.85 0.82 0.76 0.75 0.08 0 0.06 0.27 0.41 0.47 0.27 0.3 0.3
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0.12 0.2 0.18 0.16 0.24 0.35 0.26 – 0.28 0.24 0.31 0.15 0.19 0.2 0.06 0.08 0 0 0 0 0 0.05 0
Peroxisomes – 0 0.07 0.06 0.07 0 0.05 0 – 0 0 0 0.06 0.12 0.08 0 0 0 0 0 0.15 0.19 0.16 0.12
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.05 0 0 0 0 0 0.08 – 0 0 0 0 0 0.07 0.1 0 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 2 0 0 0 2 0 1 – 1 0 1 1 8 2 12 8 6 0 23 9 15 31 37
Bud – 0 0 1 0 1 1 0 – 0 1 3 1 1 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site – 0 0 0 0 2 1 1 – 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery – 2 0 1 0 1 0 4 – 0 0 0 0 0 0 3 2 1 0 0 0 0 0 0
Cytoplasm – 0 0 0 10 2 5 17 – 0 3 5 0 4 1 0 1 0 0 0 0 0 0 2
Endoplasmic Reticulum – 0 0 0 0 0 0 1 – 0 0 1 0 1 1 3 3 2 0 0 0 0 0 1
Endosome – 0 0 1 0 0 0 1 – 0 0 0 2 1 1 0 0 0 0 0 0 2 1 2
Golgi – 2 0 1 1 2 7 28 – 2 2 1 8 13 18 55 38 40 2 30 13 28 37 73
Mitochondria – 107 39 93 138 176 222 186 – 234 197 224 104 141 129 6 1 3 2 50 34 26 47 68
Nucleus – 0 0 0 0 3 1 1 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 1 0 3 0 – 0 3 1 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus – 13 8 21 26 46 99 76 – 72 53 83 19 36 35 4 4 2 0 2 1 1 8 2
Peroxisomes – 0 3 7 11 6 14 8 – 6 8 8 8 22 14 1 0 0 0 4 10 18 25 27
SpindlePole – 0 0 2 1 2 2 3 – 0 3 4 1 1 4 0 0 0 0 0 0 1 0 1
Vac/Vac Membrane – 5 0 4 0 1 8 22 – 7 3 2 4 7 12 7 2 5 0 3 0 1 3 3
Unique Cell Count 109 41 114 163 194 282 293 261 225 265 127 186 173 72 48 50 11 122 73 96 161 226
Labelled Cell Count 131 50 131 188 244 363 349 322 275 334 148 235 217 91 59 59 11 122 73 96 161 226


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 53.6 32.6 32.6 30.9 30.5 32.0 38.8 – 35.4 33.7 31.0 42.4 42.5 44.5 57.1 57.9 59.7 53.8 47.6 50.6
Std Deviation (1e-4) – 20.5 14.6 14.8 12.8 11.4 15.8 19.7 – 17.4 14.1 13.3 18.8 20.6 21.5 16.6 19.6 17.9 26.3 22.9 23.3
Intensity Change (Log2) – – – 0.0 -0.08 -0.1 -0.03 0.25 – 0.12 0.05 -0.07 0.38 0.38 0.45 0.81 0.83 0.87 0.72 0.55 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 7.8 7.5 7.7
Mitochondria -2.1 -1.8 -0.9 -2.5 -4.0 – -1.1 -1.4 -1.8 -2.1 -2.8 -2.9 -9.1 -8.8 -8.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus -0.2 -0.5 0.6 2.0 0.9 – 1.1 0.6 1.5 -0.7 0 0.1 0 -1.5 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 47.6793 64.1152 59.411 52.6267 51.9322 56.9788 48.4062 65.7019 63.0142 60.6519 53.5115 59.1685 54.9948 66.4159 60.4981 53.1956 55.7368 59.2592
Actin 0.0082 0.015 0.0113 0.01 0.0087 0.0041 0.015 0.0035 0.0024 0.0004 0.0067 0.0017 0.0059 0.0016 0.0044 0.0002 0.0006 0.0005
Bud 0.0003 0.0001 0.0001 0.0002 0 0 0.0002 0 0.0001 0 0.0034 0 0 0 0.0001 0 0 0
Bud Neck 0.0007 0.0002 0.0001 0.0001 0.0003 0 0.0004 0 0.0001 0 0.0004 0 0.0028 0 0 0 0 0
Bud Periphery 0.0005 0.0001 0.0002 0.0002 0.0001 0 0.0003 0.0001 0.0001 0 0.0035 0 0.0001 0 0.0001 0 0 0
Bud Site 0.0039 0.0002 0.0003 0.001 0.0001 0.0001 0.0013 0.0001 0.0014 0 0.0062 0.0001 0.0003 0 0.0003 0 0 0
Cell Periphery 0.0005 0.0008 0.0005 0.0002 0.0004 0.0002 0.0004 0.0002 0.0001 0 0.0002 0.0001 0.0002 0.0001 0.0002 0 0 0.0001
Cytoplasm 0.0006 0 0.0003 0.0025 0.0002 0 0.0006 0 0.0001 0 0.0103 0.0011 0.0003 0 0.0001 0 0 0
Cytoplasmic Foci 0.009 0.001 0.0034 0.0012 0.0135 0.0003 0.0284 0.0003 0.0074 0.0001 0.0381 0.0114 0.0081 0.0002 0.0118 0 0.0001 0.0107
Eisosomes 0.0064 0.0044 0.0046 0.011 0.0048 0.005 0.0017 0.0011 0.0017 0.0002 0.001 0.0007 0.0014 0.0009 0.001 0.0003 0.0007 0.0004
Endoplasmic Reticulum 0.0005 0 0.0001 0.0048 0.0012 0 0.0003 0 0 0 0.0033 0.0001 0.0002 0 0 0 0 0
Endosome 0.0014 0.001 0.0009 0.0058 0.0022 0.0002 0.006 0.0004 0.0048 0 0.0356 0.0048 0.0016 0.0001 0.0005 0 0 0.0001
Golgi 0.0019 0.0098 0.0092 0.0043 0.0046 0.0029 0.0045 0.0006 0.0008 0.0001 0.0142 0.0021 0.0023 0.001 0.0026 0.0001 0.0001 0.0006
Lipid Particles 0.0354 0.0491 0.0313 0.0302 0.0286 0.0158 0.0485 0.0089 0.0055 0.0007 0.0209 0.0136 0.019 0.0065 0.0158 0.0028 0.004 0.0073
Mitochondria 0.8882 0.8727 0.9095 0.9133 0.9004 0.9537 0.8413 0.9593 0.9576 0.9763 0.8045 0.9432 0.927 0.968 0.9383 0.9917 0.9855 0.9698
None 0.0012 0 0.0011 0.0003 0.0033 0 0.0019 0 0.0001 0 0.024 0.0004 0.0008 0 0.0001 0 0 0
Nuclear Periphery 0.0012 0.0001 0.0003 0.0041 0.0028 0 0.001 0 0.0001 0 0.0044 0.0002 0.0009 0 0.0001 0 0 0
Nucleolus 0.012 0.0025 0.0037 0.0002 0.0048 0.0017 0.0056 0.0005 0.0032 0 0.0008 0.0049 0.0062 0.0053 0.0017 0 0.0001 0.0009
Nucleus 0.0009 0.0002 0.0005 0.0011 0.0005 0.0001 0.0005 0.0001 0.0001 0 0.0035 0.0003 0.0005 0.0002 0.0003 0 0 0
Peroxisomes 0.011 0.0079 0.0055 0.0036 0.015 0.0064 0.0278 0.0078 0.008 0.0214 0.0115 0.009 0.0129 0.0119 0.0137 0.0025 0.0081 0.0048
Punctate Nuclear 0.0015 0 0.0005 0.0004 0.0007 0 0.0025 0 0.001 0 0.0016 0.0011 0.0053 0 0.0005 0 0 0.0001
Vacuole 0.0019 0.0032 0.0018 0.0006 0.0005 0.0003 0.0017 0.0026 0.0006 0.0001 0.0023 0.0004 0.0004 0.0005 0.0011 0 0.0001 0.0002
Vacuole Periphery 0.0128 0.0315 0.0146 0.005 0.0072 0.0091 0.0102 0.0144 0.0048 0.0007 0.0037 0.0047 0.0039 0.0037 0.0073 0.0023 0.0006 0.0046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 92.3257 93.9406 88.6287 113.4276 93.4277 129.0344 99.0539 121.3459 113.0588 118.4423
Translational Efficiency 1.6224 1.363 1.3549 1.294 1.5534 1.2459 1.3923 1.1792 1.3345 1.1877

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1523 1380 1576 1677 1706 1460 2514 1616 3229 2840 4090 3293

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2246.28 2335.32 3623.03 4010.13 2122.72 2563.49 3915.43 4434.85 2181.00 2452.62 3802.76 4218.56
Standard Deviation 718.67 810.61 1154.66 1204.11 662.43 811.63 1274.22 1416.91 692.28 819.11 1237.74 1329.91
Intensity Change Log 2 — 0.056082 0.689659 0.836111 — 0.272195 0.883257 1.062971 — 0.165128 0.786967 0.950791

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.012861 0.028941 0.021475 0.012809 0.016367 0.028713 0.008182 0.012483 0.014713 0.028824 0.013304 0.012649
Bud Neck 0.005885 0.004312 0.004050 0.001564 0.003383 0.004364 0.001273 0.001424 0.004563 0.004338 0.002343 0.001495
Bud Site 0.005019 0.003303 0.003383 0.002016 0.002387 0.002774 0.001778 0.002131 0.003629 0.003031 0.002397 0.002072
Cell Periphery 0.000131 0.000174 0.000105 0.000127 0.000094 0.000120 0.000092 0.000185 0.000111 0.000146 0.000097 0.000156
Cytoplasm 0.001273 0.001261 0.000861 0.000294 0.001201 0.001252 0.000412 0.000568 0.001235 0.001256 0.000585 0.000428
Cytoplasmic Foci 0.095430 0.071382 0.065104 0.013584 0.073999 0.080065 0.013082 0.016703 0.084107 0.075846 0.033128 0.015115
Eisosomes 0.009723 0.027064 0.011495 0.004101 0.016043 0.015590 0.004341 0.004415 0.013062 0.021166 0.007098 0.004255
Endoplasmic Reticulum 0.000176 0.000200 0.000094 0.000093 0.000170 0.000215 0.000030 0.000123 0.000173 0.000208 0.000055 0.000108
Endosome 0.001193 0.001453 0.001040 0.000468 0.000866 0.001388 0.000161 0.001216 0.001021 0.001419 0.000500 0.000835
Golgi 0.020627 0.025174 0.028794 0.016372 0.029564 0.042746 0.004333 0.023858 0.025349 0.034208 0.013759 0.020046
Lipid Particles 0.027254 0.019945 0.026957 0.008537 0.027176 0.029995 0.002789 0.004954 0.027213 0.025112 0.012102 0.006779
Mitochondria 0.653968* 0.623432* 0.734997* 0.908755* 0.642350* 0.658043* 0.931250* 0.905900* 0.647830* 0.641225* 0.855628* 0.907354*
Mitotic Spindle 0.006148 0.002565 0.006509 0.001490 0.003360 0.003069 0.000466 0.000820 0.004675 0.002824 0.002795 0.001161
None 0.002787 0.002948 0.003324 0.003946 0.002684 0.003013 0.001808 0.003444 0.002733 0.002981 0.002392 0.003699
Nuclear Periphery 0.000620 0.000267 0.000942 0.000832 0.000390 0.000476 0.000121 0.000302 0.000499 0.000374 0.000437 0.000572
Nuclear Periphery Foci 0.000581 0.000257 0.000347 0.000186 0.000343 0.000456 0.000045 0.000090 0.000455 0.000359 0.000161 0.000139
Nucleolus 0.005622 0.003335 0.002485 0.000926 0.002143 0.002375 0.000628 0.000896 0.003784 0.002842 0.001344 0.000911
Nucleus 0.000516 0.000634 0.000150 0.000139 0.000273 0.000488 0.000060 0.000609 0.000388 0.000559 0.000095 0.000370
Peroxisomes 0.140317* 0.178015* 0.077968 0.009564 0.172779* 0.118636* 0.026591 0.010983 0.157468* 0.147489* 0.046388 0.010261
Vacuole 0.006414 0.003629 0.004572 0.003841 0.002822 0.003931 0.001091 0.003402 0.004517 0.003784 0.002432 0.003626
Vacuole Periphery 0.003454 0.001711 0.005348 0.010356 0.001604 0.002292 0.001467 0.005495 0.002476 0.002010 0.002962 0.007970

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.55 -4.36 0.03 5.88 3.74 -4.75 4.64 1.98 6.43 -2.61 -7.85 1.10 1.48 8.67 0.48
Bud Neck 2.16 2.60 6.50 5.60 5.82 -1.36 3.89 3.99 4.87 -0.56 0.48 5.03 7.52 7.25 2.97
Bud Site 2.05 1.74 3.53 1.71 1.64 -0.88 1.02 0.12 0.81 -0.76 1.30 2.33 2.95 1.85 0.53
Cell Periphery -0.95 1.02 0.20 1.03 -0.79 -1.52 -0.03 -3.25 -2.28 -2.67 -1.49 0.69 -2.52 -0.48 -2.58
Cytoplasm 0.12 4.17 10.20 8.20 8.47 -0.40 5.75 3.80 3.77 -1.44 -0.25 7.27 8.72 7.83 1.72
Cytoplasmic Foci 4.73 6.10 19.92 16.43 15.62 -1.43 20.00 17.28 17.67 -2.08 2.51 18.37 26.44 24.21 10.29
Eisosomes -9.94 -1.78 6.96 13.74 8.60 0.34 11.75 11.55 11.03 -0.15 -7.34 8.68 13.37 17.40 6.40
Endoplasmic Reticulum -1.00 5.62 4.95 4.81 0.11 -1.77 7.94 1.99 3.45 -5.65 -2.01 10.24 4.47 5.70 -5.28
Endosome -0.60 0.91 5.52 2.32 4.15 -3.22 10.29 -1.11 0.50 -3.39 -1.77 6.52 1.09 2.18 -2.05
Golgi -2.03 -3.69 2.06 3.82 5.46 -4.59 14.26 2.12 6.08 -9.16 -4.79 8.63 3.12 7.29 -4.26
Lipid Particles 2.79 0.10 7.84 5.91 8.26 -0.97 12.81 11.20 10.68 -2.73 1.07 9.38 13.21 11.94 5.11
Mitochondria 2.40 -7.04 -25.88* -27.64* -19.38* -1.30 -33.23* -28.03* -25.03* 4.61 0.77 -29.51 -38.19* -37.21* -10.07
Mitotic Spindle 2.94 -0.26 4.26 1.22 4.69 0.30 5.37 4.53 4.58 -3.04 2.62 2.85 5.82 3.35 3.57
None -0.82 -3.26 -5.69 -3.83 -2.70 -2.26 7.42 -3.93 -1.98 -8.56 -2.05 3.55 -6.88 -4.25 -8.93
Nuclear Periphery 6.43 -3.64 -1.11 -3.04 0.55 -1.40 5.80 1.48 3.14 -4.81 3.02 1.36 -0.73 -2.02 -1.35
Nuclear Periphery Foci 4.38 3.02 5.27 1.37 2.89 -0.58 5.81 4.85 1.88 -4.00 0.87 6.65 6.97 2.13 0.86
Nucleolus 2.45 3.67 5.81 4.46 4.22 -0.63 5.38 3.87 4.26 -1.75 1.87 5.79 6.85 6.08 2.35
Nucleus -0.51 3.40 3.29 2.31 0.16 -1.19 2.47 -1.40 -0.57 -1.96 -1.25 4.30 -0.46 0.49 -1.98
Peroxisomes -4.52 9.46 23.88 25.87 17.03* 7.06 24.24 27.37* 20.77 7.40 1.75 25.32 36.25* 32.88 17.70
Vacuole 3.02 1.90 2.67 -0.26 0.86 -1.86 4.68 -0.90 0.62 -3.66 1.36 4.44 1.49 0.23 -2.34
Vacuole Periphery 4.15 -2.06 -5.28 -6.84 -3.32 -2.44 0.51 -6.75 -5.33 -6.74 1.89 -1.20 -7.61 -8.40 -6.43
Standard name
Human Ortholog
Description Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase; ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex, which in turn determines the shape of inner membrane cristae; phosphorylated
Localization
Cell Percentages mitochondrion (92%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Atp4

Atp4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atp4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available