Standard name
Human Ortholog
Description Geranylgeranyl diphosphate synthase (GGPS); increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.15 0.16 0.12 0.05 0 0.05 0.06 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.17 0.26 0.29 0.2 0.19 0 0.06 0 0.08 0.1 0.08
Mitochondria 0.91 0.88 0.97 0.94 0.92 0.91 0.91 0.87 0.92 0.92 0.94 0.95 0.76 0.78 0.66 0.08 0.08 0.11 0.81 0.78 0.78 0.68 0.69 0.66
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0.06 0.11 0.11 0.09 0.12 0.11 0 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.05 0.07 0.11 0.09 0.07 0.07 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0.12 0.18 0.16 0.07 0 0.07 0.11 0.07 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.12 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.11 0 0 0.05 0 0 0 0 0 0 0 0.16 0.13 0.19 0.13 0.17 0.23 0 0 0 0 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 0 0 0 0 0 2 0 0 0 0 5 4 2 2 7 5 0 2 1 2 4 8
Bud 3 1 3 8 7 8 14 6 10 17 17 17 2 3 2 4 8 6 0 0 0 0 0 3
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 1 1 0 4 1 6 6 5 2 5 0 0 0 0 2 3 – – – – – –
Cell Periphery 5 12 2 7 2 2 1 6 0 6 2 6 5 7 14 11 6 7 0 0 0 0 0 1
Cytoplasm 0 7 3 8 10 17 11 25 15 12 10 9 3 7 18 4 8 7 0 5 0 0 0 2
Endoplasmic Reticulum 4 8 1 2 7 8 10 6 7 10 8 14 6 2 4 5 7 3 0 0 0 2 3 2
Endosome 13 4 0 0 9 1 4 5 2 7 1 1 6 19 25 17 25 13 13 8 7 12 13 11
Golgi 10 9 1 1 0 0 0 0 0 0 0 1 36 48 86 33 31 21 7 15 3 16 29 26
Mitochondria 231 217 123 253 410 376 509 530 418 456 341 574 134 223 221 9 12 12 217 202 108 142 205 218
Nucleus 2 1 0 0 2 7 4 11 5 4 2 3 1 1 0 3 3 3 0 0 0 1 0 0
Nuclear Periphery 5 3 4 11 26 44 63 54 53 52 16 83 2 5 7 1 3 4 3 1 3 0 4 0
Nucleolus 8 2 5 7 24 30 62 55 32 35 16 44 3 3 2 5 5 2 0 0 0 0 1 3
Peroxisomes 4 2 0 4 6 2 3 7 2 8 1 3 10 16 13 14 28 18 18 9 10 22 19 17
SpindlePole 3 2 0 2 2 4 1 7 5 13 2 2 5 6 3 14 18 12 1 0 0 0 0 1
Vac/Vac Membrane 33 28 5 5 21 8 20 25 13 13 12 6 29 38 64 15 26 26 3 10 0 7 13 24
Unique Cell Count 254 248 127 269 446 415 557 607 452 495 363 602 176 286 337 115 153 113 267 260 139 208 300 330
Labelled Cell Count 323 297 148 309 526 511 703 745 569 638 430 768 247 383 461 137 189 142 267 260 139 208 300 330


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 8.1 5.2 5.5 5.6 5.6 5.2 5.7 5.4 5.3 5.4 5.4 8.2 7.6 8.0 7.1 7.1 7.2 9.3 9.7 9.8
Std Deviation (1e-4) 1.0 1.5 1.1 1.5 1.5 1.7 1.7 1.7 2.0 1.7 1.8 1.7 1.5 1.5 1.6 1.3 1.3 1.5 2.3 2.3 2.2
Intensity Change (Log2) – – – 0.07 0.1 0.12 -0.01 0.12 0.06 0.03 0.05 0.05 0.65 0.55 0.61 0.45 0.45 0.46 0.83 0.89 0.92


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 1.1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.8 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 1.4 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 3.0 3.2 4.5 4.8 3.9
Golgi 0 0 0 0 0 0 0 0 0 5.2 4.6 6.1 6.2 5.1 4.8
Mitochondria -1.2 -1.9 -2.3 -2.1 -3.1 -1.8 -1.9 -1.3 -0.8 -5.0 -4.8 -6.9 -13.9 -14.8 -13.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1.2 2.6 2.8 2.2 2.9 2.6 0.6 3.4 0 0 0 0 0 0
Nucleolus 0 0.7 1.3 2.5 1.9 1.3 1.3 0.2 1.4 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 4.1 5.1 4.7
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 4.1 4.0 3.8
Vacuole 0 0.4 0 0 0.1 0 0 0 0 3.4 2.9 4.1 2.6 3.5 4.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.5497 8.6794 8.9298 8.2817 7.7434 8.6713 8.7596 7.9767 8.7817 7.779 7.8296 8.6678 5.4961 6.7718 5.789 6.5152 4.6244 6.8445
Actin 0.0011 0.004 0.0008 0 0.0005 0.0004 0.0009 0.0027 0.0004 0.0013 0.0001 0.0003 0.0093 0.0007 0.0107 0.0017 0.0004 0.0001
Bud 0 0.0002 0 0 0 0 0.0001 0.0002 0 0.0003 0 0 0.0001 0 0.0006 0.0002 0.0001 0
Bud Neck 0 0.0001 0 0 0 0 0 0 0 0 0 0 0.0002 0 0.0004 0.0001 0.0001 0
Bud Periphery 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0 0.0011 0.0003 0 0.0002 0.0001 0.0011 0.0002 0.0002 0
Bud Site 0 0.0003 0 0 0 0 0.0001 0.0004 0 0.0001 0 0 0.0003 0.0002 0.0049 0.0002 0.0003 0
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0 0 0 0.0001 0.0001 0.0002 0.0001 0.0001 0
Cytoplasm 0 0.0001 0 0 0 0 0 0 0 0 0 0 0.0002 0 0.0001 0.0001 0.0001 0
Cytoplasmic Foci 0.0012 0.0025 0.0027 0 0.0003 0.0008 0.0009 0.0024 0.0007 0.0002 0.0001 0.0004 0.0196 0.0012 0.0064 0.0078 0.0042 0
Eisosomes 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0001
Endoplasmic Reticulum 0.0001 0.0001 0 0 0 0.0001 0.0001 0 0 0 0 0 0.0005 0.0001 0.0001 0 0.0001 0
Endosome 0.0041 0.0089 0.0016 0.0004 0.0022 0.0046 0.0036 0.0052 0.0028 0.0005 0.0006 0.0009 0.013 0.0032 0.0156 0.0053 0.0185 0.0003
Golgi 0.0106 0.038 0.0106 0.0007 0.0039 0.0194 0.0074 0.0157 0.0123 0.0014 0.001 0.0041 0.0271 0.0094 0.0224 0.0097 0.0091 0.0005
Lipid Particles 0.0043 0.0015 0.0004 0.0001 0.0005 0.0014 0.0113 0.0039 0.0093 0.0001 0.0007 0.0019 0.0437 0.0057 0.0135 0.0432 0.0272 0.0025
Mitochondria 0.9687 0.9357 0.9752 0.9975 0.9838 0.9695 0.9528 0.95 0.9699 0.9941 0.9949 0.984 0.8524 0.9569 0.8955 0.8392 0.9241 0.9722
None 0 0 0 0 0 0 0 0 0 0 0 0 0.0003 0 0.0001 0 0.0001 0
Nuclear Periphery 0.0001 0 0 0 0 0.0004 0.0005 0 0 0 0 0 0.0081 0 0.0001 0 0.0001 0.0004
Nucleolus 0 0 0 0 0 0 0.0001 0 0 0 0 0 0.0002 0.0001 0.0002 0.0001 0.0003 0
Nucleus 0 0 0 0 0 0 0.0002 0 0 0 0 0 0.0002 0 0.0001 0 0.0001 0
Peroxisomes 0.0071 0.0059 0.0032 0.0001 0.0007 0.0014 0.014 0.0166 0.0023 0.0006 0.0015 0.0035 0.0166 0.0145 0.0234 0.0869 0.01 0.0003
Punctate Nuclear 0 0 0 0 0 0 0 0 0 0 0 0 0.0015 0 0.0001 0.0003 0.0001 0
Vacuole 0.0004 0.0007 0.0016 0.0001 0.0014 0.0003 0.0025 0.0004 0.0007 0.0001 0.0002 0.0022 0.0012 0.0014 0.0014 0.0007 0.0025 0.001
Vacuole Periphery 0.0016 0.0015 0.0033 0.0006 0.0063 0.0015 0.0051 0.0019 0.0013 0.0002 0.0004 0.0024 0.0051 0.0062 0.0028 0.0042 0.0022 0.0225

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.6625 9.7855 7.9874 13.9649 7.0963 12.0345 10.1414 9.6829 8.7144 12.5123
Translational Efficiency 1.0494 0.8191 0.9339 0.6099 1.2087 0.5674 0.9697 0.6877 1.2495 0.9214

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
130 1497 233 101 1609 1581 821 1754 1739 3078 1054 1855

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 837.38 881.11 1319.86 1222.34 830.14 893.05 1166.00 1124.81 830.68 887.24 1200.01 1130.12
Standard Deviation 117.96 194.17 188.83 157.75 170.79 149.48 174.59 165.86 167.43 172.77 188.95 166.90
Intensity Change Log 2 — 0.073440 0.656431 0.545691 — 0.105386 0.490141 0.438255 — 0.089432 0.576041 0.493206

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001347 0.003963 0.017651 0.021628 0.001895 0.001505 0.004899 0.015355 0.001854 0.002701 0.007718 0.015697
Bud Neck 0.006566 0.003470 0.009356 0.002625 0.003177 0.000773 0.002221 0.001190 0.003430 0.002084 0.003798 0.001268
Bud Site 0.017527 0.007267 0.017550 0.014045 0.005429 0.002894 0.006564 0.007595 0.006334 0.005020 0.008992 0.007946
Cell Periphery 0.000790 0.000357 0.000494 0.000568 0.001107 0.000287 0.000223 0.001370 0.001083 0.000321 0.000283 0.001326
Cytoplasm 0.002492 0.001017 0.002797 0.001048 0.000749 0.000796 0.001599 0.001395 0.000880 0.000904 0.001863 0.001376
Cytoplasmic Foci 0.050765 0.028359 0.073609 0.050065 0.009409 0.014331 0.024149 0.010166 0.012501 0.021153 0.035083 0.012338
Eisosomes 0.000088 0.000087 0.000207 0.000197 0.000114 0.000051 0.000100 0.000142 0.000112 0.000068 0.000124 0.000145
Endoplasmic Reticulum 0.001884 0.000362 0.002035 0.001119 0.000429 0.000165 0.000364 0.000433 0.000538 0.000261 0.000733 0.000470
Endosome 0.088996 0.043147 0.053266 0.049307 0.014830 0.021220 0.017113 0.015120 0.020374 0.031884 0.025106 0.016982
Golgi 0.068914 0.040094 0.131100* 0.173022* 0.015716 0.023945 0.033613 0.031331 0.019693 0.031799 0.055164 0.039046
Lipid Particles 0.008237 0.006845 0.006468 0.002729 0.007518 0.002807 0.003158 0.000518 0.007572 0.004771 0.003890 0.000638
Mitochondria 0.601114* 0.812026* 0.546166* 0.620465* 0.904128* 0.901850* 0.857159* 0.884450* 0.881476* 0.858164* 0.788410* 0.870077*
Mitotic Spindle 0.009670 0.003898 0.041002 0.011171 0.001007 0.002010 0.007621 0.003377 0.001655 0.002928 0.015001 0.003802
None 0.000378 0.000342 0.000628 0.000336 0.000552 0.000190 0.000420 0.000264 0.000539 0.000264 0.000466 0.000268
Nuclear Periphery 0.001075 0.000155 0.001147 0.000299 0.000195 0.000078 0.000533 0.000062 0.000260 0.000116 0.000669 0.000075
Nuclear Periphery Foci 0.001829 0.000319 0.001277 0.000285 0.000376 0.000118 0.000781 0.000052 0.000485 0.000216 0.000890 0.000065
Nucleolus 0.005090 0.000331 0.000687 0.000038 0.000719 0.000434 0.000200 0.000026 0.001046 0.000384 0.000308 0.000027
Nucleus 0.003270 0.000350 0.000520 0.000065 0.000649 0.000325 0.000087 0.000024 0.000845 0.000337 0.000183 0.000026
Peroxisomes 0.074907 0.037712 0.055595 0.039511 0.024459 0.017242 0.023867 0.015939 0.028230 0.027198 0.030881 0.017223
Vacuole 0.018031 0.005232 0.020813 0.005756 0.003728 0.003973 0.008518 0.005349 0.004798 0.004585 0.011236 0.005371
Vacuole Periphery 0.037031 0.004666 0.017630 0.005722 0.003813 0.005005 0.006811 0.005840 0.006296 0.004840 0.009203 0.005834

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.20 -4.30 -2.69 -2.33 -0.48 0.80 -2.86 -6.42 -6.72 -4.62 -1.55 -4.89 -6.84 -6.45 -3.50
Bud Neck 1.65 -1.26 2.09 0.82 3.91 3.30 1.17 2.68 -1.73 2.77 1.76 -0.53 3.08 2.02 5.34
Bud Site 1.93 -0.05 0.35 -0.82 0.41 2.05 -0.72 -1.55 -4.19 -0.43 0.99 -1.58 -1.14 -2.37 0.71
Cell Periphery 2.60 1.73 0.92 -1.15 -0.40 3.42 3.66 -0.72 -3.80 -4.01 3.45 3.59 -0.71 -3.74 -3.87
Cytoplasm 2.56 -0.58 2.54 -0.10 1.31 -0.15 -1.47 -2.34 -1.52 0.61 -0.12 -1.87 -1.88 -1.62 1.17
Cytoplasmic Foci 3.25 -2.43 0.07 -2.41 2.14 -3.29 -5.86 -0.55 2.74 5.52 -5.84 -8.58 0.12 5.87 8.60
Eisosomes 0.09 -4.97 -3.70 -3.92 0.29 4.63 0.87 -1.54 -7.43 -2.87 3.41 -0.80 -1.93 -6.45 -1.50
Endoplasmic Reticulum 1.98 -0.15 0.96 -2.74 1.23 2.77 0.37 -0.04 -2.95 -0.41 2.36 -0.89 0.49 -2.07 1.24
Endosome 3.71 2.73 2.72 -0.73 0.42 -3.30 -1.15 -0.18 3.26 1.04 -5.47 -2.08 1.76 8.02 3.93
Golgi 2.75 -3.94 -3.87 -5.31 -1.53 -3.76 -5.22 -5.92 -2.64 0.59 -5.76 -8.65 -6.38 -2.43 3.48
Lipid Particles 0.56 0.65 1.96 2.63 2.00 3.30 2.98 5.18 4.41 4.35 2.05 2.68 5.48 6.99 5.57
Mitochondria -6.27 1.35 -0.42 4.97 -1.70 0.29 4.44 2.49 2.26 -2.61 3.04 8.16 1.34 -1.60 -7.23
Mitotic Spindle 1.16 -3.21 -0.05 -2.20 3.73 -1.49 -5.68 -4.86 -1.72 3.43 -1.77 -6.78 -3.10 -1.31 5.73
None 0.33 -3.15 0.60 0.11 5.06 6.02 2.13 4.79 -4.95 7.15 4.11 1.27 4.85 -0.06 9.37
Nuclear Periphery 1.56 -0.12 1.29 -0.93 2.95 1.25 -1.48 1.40 0.68 2.23 1.40 -2.07 1.89 0.95 3.41
Nuclear Periphery Foci 1.52 0.47 1.56 0.24 3.62 2.75 -0.92 3.57 2.58 1.67 2.11 -1.17 3.75 2.45 2.44
Nucleolus 1.53 1.40 1.62 3.15 1.46 0.57 1.41 1.94 1.30 2.69 1.49 1.74 2.52 2.13 2.56
Nucleus 1.71 1.56 1.89 2.46 1.66 0.78 1.38 1.53 1.89 3.15 1.24 1.60 2.02 3.15 2.45
Peroxisomes 2.54 1.16 1.91 -0.14 1.09 2.42 0.15 2.95 0.51 2.16 0.36 -0.65 3.69 4.02 3.73
Vacuole 3.15 -0.66 2.89 -0.33 4.00 -0.37 -3.29 -1.70 -1.46 2.02 0.30 -4.52 -0.62 -0.90 3.85
Vacuole Periphery 4.90 2.80 4.70 -0.95 5.01 -1.74 -3.00 -2.57 -1.05 0.89 1.89 -2.67 0.49 -1.46 3.24
Standard name
Human Ortholog
Description Geranylgeranyl diphosphate synthase (GGPS); increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Bts1

Bts1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bts1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available