Standard name
Description Essential component of the TIM23 complex; acts as receptor for the translocase of the inner mitochondrial membrane (TIM23) complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.11 0 0 0 0 0 0 0 0 0 0 0.14 0.1 0.2 0 0.06 0.06 0.08 0.1 0.07
Mitochondria 0.91 0.91 0.87 0.92 0.91 0.86 0.89 0.87 0.92 0.93 0.95 0.94 0.89 0.9 0.87 0.82 0.8 0.75 0.73 0.71 0.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.07 0.08 0.07 0.1 0.07 0.09 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 0 0.09 0 0.11 0.14 0.12 0.17 0.18 0.11 0.07 0.13 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.16 0 0 0.06 0 0 0 0 0 0 0 0.11 0.09 0.11 0 0.08 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 1 1 0 1 1 2 2 3 3 3 4 5 5 11
Bud 0 0 4 2 1 1 3 8 4 8 9 0 2 1 0 1 0 0 1 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 1 0 0 0 0 0 1 2 1 1 0 1 0 0 0
Cell Periphery 1 6 2 3 0 0 1 0 1 1 0 1 3 5 5 0 0 0 0 0 0
Cytoplasm 5 0 3 5 2 5 3 9 6 6 5 2 9 6 3 1 0 0 0 0 0
Endoplasmic Reticulum 0 2 0 0 1 1 0 1 2 2 0 0 5 5 1 0 0 0 0 1 1
Endosome 0 0 0 0 0 0 2 0 0 1 0 0 0 1 1 3 0 1 1 7 5
Golgi 2 21 1 3 4 1 0 0 0 0 0 0 39 34 67 8 13 6 11 26 22
Mitochondria 188 168 80 101 106 144 200 209 132 172 140 137 254 316 289 170 170 76 101 182 219
Nucleus 1 1 1 0 1 1 1 1 0 0 0 1 1 0 0 2 0 0 0 0 1
Nuclear Periphery 4 3 3 0 5 7 15 18 10 19 10 13 2 5 7 0 1 0 1 2 3
Nucleolus 21 6 8 4 13 24 28 40 26 20 10 19 7 10 3 3 1 2 1 4 2
Peroxisomes 2 0 4 0 1 1 2 3 0 0 1 0 1 7 5 1 2 7 6 10 12
SpindlePole 1 0 0 0 0 1 1 2 0 1 0 0 0 0 0 1 0 0 1 0 0
Vac/Vac Membrane 26 29 3 2 7 6 10 5 0 6 1 4 31 30 38 6 17 0 2 8 22
Unique Cell Count 207 185 92 110 117 167 224 239 143 184 147 145 285 352 334 207 213 101 140 257 312
Labelled Cell Count 252 237 109 120 141 192 267 299 183 237 177 179 356 422 422 207 213 101 140 257 312


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.2 17.6 15.0 16.1 14.9 13.9 13.1 11.5 12.0 11.1 10.4 11.6 17.8 17.9 17.4 14.5 15.4 16.1
Std Deviation (1e-4) 3.0 3.3 3.5 3.9 4.0 3.9 3.5 3.0 3.6 2.9 2.8 3.3 3.5 4.1 3.5 3.4 3.3 3.3
Intensity Change (Log2) 0.11 -0.01 -0.1 -0.19 -0.38 -0.31 -0.43 -0.53 -0.37 0.25 0.26 0.22 -0.05 0.05 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.6 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 3.4 2.7 4.4
Mitochondria 1.1 0.8 -0.2 0.6 0.1 1.3 1.8 2.3 2.0 0.6 0.8 -0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1.2 1.4 1.2 2.0 1.2 1.7 0 0 0
Nucleolus -1.5 0.6 1.3 1.0 1.9 2.0 0.6 -0.5 1.0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 2.2 1.7 2.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tim50-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available