Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial isoleucyl-tRNA synthetase; null mutant is deficient in respiratory growth; human homolog IARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 2 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 4 | 2 | 8 | 4 | 0 | 4 | 7 |
Bud | 2 | 12 | 23 | 44 | 28 | 28 | 34 | 53 | 21 | 19 | 26 | 35 | 1 | 5 | 3 | 2 | 3 | 6 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 1 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Bud Site | 0 | 2 | 5 | 5 | 1 | 9 | 11 | 18 | 3 | 6 | 8 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 25 | 8 | 9 | 2 | 4 | 3 | 4 | 4 | 1 | 4 | 5 | 5 | 4 | 3 | 11 | 8 | 8 | 0 | 1 | 0 | 0 | 0 | 1 |
Cytoplasm | 4 | 18 | 22 | 22 | 21 | 24 | 35 | 50 | 18 | 19 | 25 | 23 | 4 | 4 | 9 | 16 | 19 | 15 | 1 | 2 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 3 | 6 | 6 | 1 | 1 | 3 | 4 | 4 | 3 | 0 | 7 | 0 | 2 | 5 | 3 | 9 | 9 | 0 | 0 | 2 | 0 | 1 | 1 | 0 |
Endosome | 43 | 18 | 0 | 1 | 9 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 9 | 16 | 4 | 16 | 22 | 17 | 30 | 36 | 35 | 5 | 8 | 7 |
Golgi | 24 | 8 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 9 | 6 | 39 | 50 | 30 | 13 | 29 | 4 | 4 | 15 | 16 |
Mitochondria | 242 | 492 | 389 | 426 | 407 | 465 | 690 | 737 | 379 | 420 | 345 | 417 | 118 | 90 | 129 | 15 | 17 | 13 | 286 | 441 | 328 | 64 | 129 | 97 |
Nucleus | 6 | 1 | 2 | 3 | 4 | 7 | 14 | 16 | 3 | 3 | 3 | 3 | 0 | 1 | 0 | 0 | 4 | 3 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 17 | 14 | 2 | 0 | 2 | 13 | 19 | 4 | 8 | 7 | 7 | 2 | 2 | 2 | 2 | 2 | 1 | 2 | 2 | 4 | 4 | 1 | 2 | 2 |
Nucleolus | 6 | 4 | 1 | 1 | 3 | 6 | 4 | 4 | 1 | 2 | 3 | 4 | 1 | 0 | 2 | 3 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 |
Peroxisomes | 11 | 23 | 7 | 4 | 13 | 1 | 3 | 7 | 4 | 2 | 2 | 4 | 4 | 10 | 1 | 16 | 1 | 9 | 23 | 18 | 21 | 3 | 16 | 12 |
SpindlePole | 15 | 9 | 16 | 19 | 26 | 20 | 35 | 50 | 11 | 11 | 14 | 24 | 0 | 4 | 0 | 11 | 21 | 4 | 0 | 7 | 3 | 0 | 1 | 0 |
Vac/Vac Membrane | 51 | 100 | 19 | 6 | 28 | 18 | 28 | 20 | 13 | 9 | 11 | 13 | 25 | 17 | 26 | 29 | 28 | 16 | 5 | 26 | 4 | 3 | 3 | 4 |
Unique Cell Count | 311 | 559 | 411 | 449 | 446 | 499 | 736 | 795 | 396 | 435 | 371 | 441 | 135 | 126 | 152 | 149 | 148 | 99 | 372 | 589 | 418 | 87 | 187 | 158 |
Labelled Cell Count | 431 | 734 | 505 | 544 | 547 | 600 | 882 | 968 | 468 | 499 | 456 | 537 | 175 | 168 | 189 | 169 | 187 | 128 | 372 | 589 | 418 | 87 | 187 | 158 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 5.1 | 3.8 | 4.2 | 3.9 | 3.7 | 3.4 | 3.4 | 3.0 | 3.0 | 3.1 | 3.0 | 5.8 | 5.8 | 5.0 | 7.6 | 7.9 | 7.6 | 5.2 | 6.6 | 7.2 |
Std Deviation (1e-4) | 0.8 | 1.1 | 0.7 | 3.4 | 1.0 | 1.1 | 0.8 | 0.9 | 0.8 | 1.0 | 1.3 | 3.4 | 1.0 | 1.0 | 0.8 | 1.9 | 1.9 | 2.2 | 0.9 | 2.0 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.16 | 0.05 | -0.04 | -0.14 | -0.15 | -0.33 | -0.33 | -0.28 | -0.31 | 0.62 | 0.63 | 0.42 | 1.01 | 1.07 | 1.02 | 0.47 | 0.81 | 0.93 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.4 | -0.7 | -1.8 | -2.2 | -1.8 | 0.2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 |
Cytoplasm | -0.3 | -0.4 | -0.4 | -0.4 | 0.7 | -0.5 | -0.7 | 0.8 | -0.1 | -1.1 | -1.0 | 0.3 | 2.2 | 3.0 | 3.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8.0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10.5 | 12.2 | 11.2 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.8 | -7.3 | -3.8 | -19.7 | -19.5 | -17.8 |
Nucleus | 0 | 0 | 0 | 2.0 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.8 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 4.3 | 0 |
Vacuole | -2.9 | 1.1 | -0.8 | -0.7 | -2.0 | -1.0 | -2.1 | -1.2 | -1.3 | 5.1 | 3.5 | 4.8 | 5.5 | 5.4 | 4.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3663 | 2.408 | 2.6216 | 2.2797 | 2.2563 | 2.6328 | 4.1682 | 4.4495 | 4.4082 | 4.6338 | 5.136 | 4.3383 | 4.5758 | 4.4453 | 4.8244 | 5.1958 | 4.9478 | 4.8361 |
Actin | 0.0172 | 0.016 | 0.0159 | 0.0137 | 0.0033 | 0.0092 | 0.0146 | 0.0134 | 0.0126 | 0.0077 | 0.0339 | 0.0042 | 0.028 | 0.0256 | 0.0194 | 0.0018 | 0.0099 | 0.0099 |
Bud | 0.0005 | 0.0012 | 0.0019 | 0.0007 | 0.0014 | 0.0008 | 0.001 | 0.0024 | 0.0013 | 0.0017 | 0.0049 | 0.0005 | 0.0006 | 0.0019 | 0.0009 | 0.0033 | 0.0006 | 0.0006 |
Bud Neck | 0.0009 | 0.0007 | 0.0018 | 0.0022 | 0.0003 | 0.0016 | 0.0052 | 0.001 | 0.0011 | 0.0007 | 0.0097 | 0.0008 | 0.0008 | 0.0005 | 0.0011 | 0.0007 | 0.0003 | 0.0005 |
Bud Periphery | 0.0012 | 0.0024 | 0.006 | 0.0015 | 0.0046 | 0.0035 | 0.0019 | 0.0045 | 0.0026 | 0.0134 | 0.0099 | 0.002 | 0.0014 | 0.0022 | 0.0023 | 0.0094 | 0.003 | 0.0017 |
Bud Site | 0.0018 | 0.0102 | 0.0066 | 0.002 | 0.0017 | 0.001 | 0.0049 | 0.0214 | 0.0138 | 0.0006 | 0.007 | 0.0006 | 0.0056 | 0.0096 | 0.0074 | 0.0017 | 0.0008 | 0.0008 |
Cell Periphery | 0.0005 | 0.0026 | 0.0033 | 0.0003 | 0.0008 | 0.0005 | 0.0012 | 0.0009 | 0.0009 | 0.0006 | 0.0012 | 0.0007 | 0.0007 | 0.0004 | 0.0064 | 0.0013 | 0.003 | 0.0006 |
Cytoplasm | 0.003 | 0.0027 | 0.0042 | 0.0003 | 0.0095 | 0.0015 | 0.0123 | 0.012 | 0.0065 | 0.0018 | 0.0153 | 0.0043 | 0.0029 | 0.0027 | 0.0054 | 0.0226 | 0.004 | 0.0034 |
Cytoplasmic Foci | 0.0182 | 0.0315 | 0.0177 | 0.0117 | 0.0156 | 0.0172 | 0.0302 | 0.025 | 0.0242 | 0.0171 | 0.0279 | 0.0239 | 0.0234 | 0.0473 | 0.0333 | 0.0203 | 0.0232 | 0.0233 |
Eisosomes | 0.0004 | 0.0007 | 0.0009 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0.0003 | 0.0011 | 0.0003 | 0.0008 | 0.0002 | 0.0012 | 0.0005 | 0.0016 | 0.0002 | 0.0015 | 0.0004 |
Endoplasmic Reticulum | 0.0037 | 0.0006 | 0.0021 | 0.0006 | 0.0022 | 0.001 | 0.003 | 0.001 | 0.0011 | 0.001 | 0.0028 | 0.0014 | 0.0006 | 0.0003 | 0.0011 | 0.0012 | 0.0004 | 0.0024 |
Endosome | 0.0643 | 0.0576 | 0.0524 | 0.0222 | 0.0569 | 0.0474 | 0.0854 | 0.0755 | 0.0464 | 0.0417 | 0.1085 | 0.0556 | 0.0507 | 0.0489 | 0.0467 | 0.083 | 0.0258 | 0.0429 |
Golgi | 0.0457 | 0.0732 | 0.0479 | 0.024 | 0.0597 | 0.0385 | 0.0519 | 0.067 | 0.0337 | 0.0461 | 0.0809 | 0.0538 | 0.0813 | 0.1056 | 0.0417 | 0.0336 | 0.0669 | 0.0719 |
Lipid Particles | 0.0379 | 0.0354 | 0.0174 | 0.0167 | 0.0034 | 0.0166 | 0.0317 | 0.0121 | 0.0057 | 0.0039 | 0.004 | 0.0111 | 0.0204 | 0.0128 | 0.0067 | 0.0022 | 0.0068 | 0.0141 |
Mitochondria | 0.7458 | 0.7183 | 0.7832 | 0.8809 | 0.7923 | 0.8161 | 0.6882 | 0.7231 | 0.7984 | 0.8362 | 0.6492 | 0.8056 | 0.7241 | 0.6616 | 0.7891 | 0.7842 | 0.7886 | 0.785 |
None | 0.0025 | 0.0014 | 0.001 | 0.0003 | 0.0011 | 0.0011 | 0.0227 | 0.0012 | 0.0136 | 0.002 | 0.0029 | 0.003 | 0.0063 | 0.0006 | 0.0045 | 0.0015 | 0.0032 | 0.0023 |
Nuclear Periphery | 0.0064 | 0.0002 | 0.0018 | 0.0008 | 0.0025 | 0.0057 | 0.0067 | 0.0003 | 0.0003 | 0.0028 | 0.0084 | 0.0018 | 0.0001 | 0.0001 | 0.0006 | 0.0004 | 0.0001 | 0.0004 |
Nucleolus | 0.0005 | 0.0003 | 0.0006 | 0.0005 | 0.0003 | 0.0007 | 0.0008 | 0.0008 | 0.0007 | 0.0001 | 0.0014 | 0.0003 | 0.0004 | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0001 |
Nucleus | 0.0015 | 0.0002 | 0.0008 | 0.0003 | 0.0032 | 0.0008 | 0.0015 | 0.0006 | 0.0007 | 0.0004 | 0.005 | 0.0018 | 0.0002 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0.0001 |
Peroxisomes | 0.0327 | 0.0387 | 0.0247 | 0.0162 | 0.033 | 0.0282 | 0.0215 | 0.0191 | 0.0261 | 0.0143 | 0.0064 | 0.0199 | 0.0469 | 0.0761 | 0.0259 | 0.0094 | 0.0592 | 0.0362 |
Punctate Nuclear | 0.0009 | 0.0002 | 0.0007 | 0.001 | 0.0006 | 0.0007 | 0.0016 | 0.0002 | 0.0002 | 0.0001 | 0.0027 | 0.0013 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Vacuole | 0.0075 | 0.0029 | 0.005 | 0.0021 | 0.0037 | 0.0033 | 0.007 | 0.0123 | 0.0062 | 0.0028 | 0.0102 | 0.0038 | 0.0021 | 0.0014 | 0.0027 | 0.0127 | 0.0013 | 0.0014 |
Vacuole Periphery | 0.0068 | 0.003 | 0.0042 | 0.0019 | 0.0037 | 0.0044 | 0.0061 | 0.0059 | 0.0028 | 0.0047 | 0.0071 | 0.0035 | 0.002 | 0.0016 | 0.002 | 0.0096 | 0.0011 | 0.0019 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.6006 | 3.9337 | 8.0272 | 9.5375 | 8.3203 | 9.8955 | 9.4462 | 12.863 | 10.0424 | 10.7616 |
Translational Efficiency | 0.537 | 0.8306 | 0.3474 | 0.3126 | 0.3335 | 0.3446 | 0.4433 | 0.3469 | 0.403 | 0.3589 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
340 | 206 | 1912 | 254 | 869 | 1609 | 939 | 75 | 1209 | 1815 | 2851 | 329 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 648.49 | 773.67 | 1037.18 | 954.34 | 667.65 | 805.66 | 964.79 | 967.69 | 662.26 | 802.03 | 1013.34 | 957.38 |
Standard Deviation | 82.95 | 104.84 | 151.34 | 115.22 | 103.87 | 109.04 | 128.45 | 123.24 | 98.81 | 109.04 | 148.17 | 117.23 |
Intensity Change Log 2 | — | 0.254634 | 0.677510 | 0.557419 | — | 0.271079 | 0.531123 | 0.535453 | — | 0.263000 | 0.605107 | 0.546318 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000839 | 0.003436 | 0.002940 | 0.020455 | 0.002444 | 0.002033 | 0.012315 | 0.023413 | 0.001993 | 0.002192 | 0.006028 | 0.021130 |
Bud Neck | 0.024000 | 0.004431 | 0.002159 | 0.012459 | 0.008154 | 0.001456 | 0.007640 | 0.013419 | 0.012610 | 0.001794 | 0.003964 | 0.012678 |
Bud Site | 0.023624 | 0.010368 | 0.019115 | 0.119484* | 0.006225 | 0.006105 | 0.046950 | 0.134224* | 0.011118 | 0.006589 | 0.028282 | 0.122844* |
Cell Periphery | 0.001163 | 0.000554 | 0.000222 | 0.000425 | 0.000754 | 0.000282 | 0.000312 | 0.000937 | 0.000869 | 0.000313 | 0.000251 | 0.000542 |
Cytoplasm | 0.011447 | 0.003704 | 0.003639 | 0.023867 | 0.003713 | 0.002267 | 0.009897 | 0.015429 | 0.005888 | 0.002430 | 0.005700 | 0.021944 |
Cytoplasmic Foci | 0.038973 | 0.054099 | 0.059611 | 0.091758 | 0.031822 | 0.034203 | 0.104219 | 0.100456 | 0.033833 | 0.036461 | 0.074303 | 0.093740 |
Eisosomes | 0.000116 | 0.000113 | 0.000054 | 0.000138 | 0.000081 | 0.000062 | 0.000128 | 0.000197 | 0.000091 | 0.000068 | 0.000079 | 0.000151 |
Endoplasmic Reticulum | 0.000730 | 0.000344 | 0.001089 | 0.002107 | 0.000943 | 0.000223 | 0.002894 | 0.003014 | 0.000883 | 0.000237 | 0.001684 | 0.002314 |
Endosome | 0.075347 | 0.110032 | 0.080658 | 0.085390 | 0.084430 | 0.077378 | 0.116986 | 0.116531 | 0.081876 | 0.081084 | 0.092623 | 0.092489 |
Golgi | 0.075014 | 0.095427 | 0.141727* | 0.108038 | 0.059227 | 0.101107 | 0.107068 | 0.083371 | 0.063667 | 0.100462 | 0.130312* | 0.102415 |
Lipid Particles | 0.006467 | 0.010834 | 0.000853 | 0.000599 | 0.006734 | 0.002147 | 0.003425 | 0.001022 | 0.006659 | 0.003133 | 0.001700 | 0.000696 |
Mitochondria | 0.651407* | 0.537995* | 0.470896* | 0.230597* | 0.720611* | 0.713277* | 0.255851* | 0.222390* | 0.701149* | 0.693383* | 0.400069* | 0.228726* |
Mitotic Spindle | 0.005711 | 0.023209 | 0.071585 | 0.096564 | 0.004842 | 0.006376 | 0.153529* | 0.065285 | 0.005086 | 0.008287 | 0.098574 | 0.089434 |
None | 0.000954 | 0.000241 | 0.000393 | 0.000215 | 0.000454 | 0.000425 | 0.000429 | 0.000130 | 0.000595 | 0.000404 | 0.000405 | 0.000195 |
Nuclear Periphery | 0.000194 | 0.000095 | 0.000738 | 0.000179 | 0.000097 | 0.000059 | 0.000687 | 0.000144 | 0.000124 | 0.000063 | 0.000721 | 0.000171 |
Nuclear Periphery Foci | 0.001388 | 0.000152 | 0.001184 | 0.000321 | 0.000491 | 0.000155 | 0.001478 | 0.000411 | 0.000743 | 0.000154 | 0.001281 | 0.000341 |
Nucleolus | 0.001572 | 0.001231 | 0.000411 | 0.000459 | 0.000677 | 0.000186 | 0.001389 | 0.000098 | 0.000929 | 0.000305 | 0.000733 | 0.000377 |
Nucleus | 0.003867 | 0.006301 | 0.001069 | 0.003756 | 0.001290 | 0.000208 | 0.005301 | 0.000548 | 0.002015 | 0.000900 | 0.002463 | 0.003025 |
Peroxisomes | 0.032751 | 0.123332* | 0.015556 | 0.132136* | 0.052517 | 0.031213 | 0.093478 | 0.164449* | 0.046958 | 0.041668 | 0.041220 | 0.139502* |
Vacuole | 0.018767 | 0.008915 | 0.061251 | 0.057302 | 0.005279 | 0.009080 | 0.057166 | 0.046230 | 0.009072 | 0.009061 | 0.059906 | 0.054778 |
Vacuole Periphery | 0.025670 | 0.005189 | 0.064849 | 0.013750 | 0.009216 | 0.011758 | 0.018857 | 0.008302 | 0.013843 | 0.011013 | 0.049701 | 0.012508 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.41 | -3.68 | -4.68 | -3.95 | -4.15 | 0.33 | -5.23 | -2.43 | -2.49 | -1.27 | -0.21 | -4.07 | -4.98 | -4.97 | -3.95 |
Bud Neck | 4.78 | 5.50 | 2.44 | -3.19 | -4.39 | 3.96 | -0.06 | -1.33 | -3.37 | -1.41 | 6.49 | 5.00 | -0.10 | -5.49 | -4.27 |
Bud Site | 2.93 | 1.01 | -7.49 | -8.74 | -8.12 | 0.10 | -13.33 | -5.66 | -5.67 | -3.77 | 3.07 | -9.42 | -10.31 | -10.78 | -8.66 |
Cell Periphery | 1.40 | 2.70 | 2.09 | 0.49 | -2.87 | 2.97 | 2.83 | -0.58 | -2.46 | -2.37 | 3.60 | 4.16 | 1.97 | -2.45 | -3.53 |
Cytoplasm | 2.52 | 2.65 | -2.82 | -5.82 | -6.03 | 1.17 | -4.34 | -3.27 | -3.88 | -1.35 | 2.79 | 0.02 | -5.45 | -7.13 | -5.88 |
Cytoplasmic Foci | -2.29 | -4.27 | -5.93 | -4.06 | -3.98 | -0.75 | -15.62 | -4.56 | -4.44 | 0.15 | -0.90 | -13.68 | -8.29 | -8.12 | -2.78 |
Eisosomes | 0.20 | 5.20 | -1.23 | -1.35 | -7.04 | 2.76 | -5.44 | -2.82 | -3.33 | -1.65 | 3.69 | 1.90 | -4.24 | -6.33 | -5.47 |
Endoplasmic Reticulum | 1.70 | -1.83 | -2.01 | -2.58 | -1.50 | 1.12 | -2.88 | -1.03 | -1.47 | 0.16 | 1.39 | -1.93 | -1.79 | -3.08 | -0.74 |
Endosome | -2.75 | -0.67 | -0.85 | 2.03 | -0.47 | 1.11 | -4.27 | -1.71 | -2.15 | -0.07 | 0.15 | -2.03 | -1.16 | -1.35 | 0.05 |
Golgi | -1.81 | -7.76 | -2.62 | -0.86 | 3.44 | -7.42 | -7.35 | -1.89 | 1.43 | 1.62 | -7.23 | -13.49 | -4.39 | -0.04 | 3.37 |
Lipid Particles | -0.88 | 2.67 | 2.78 | 2.28 | 1.36 | 3.37 | 2.28 | 3.98 | 1.26 | 2.92 | 2.75 | 4.49 | 5.47 | 3.33 | 3.90 |
Mitochondria | 3.54 | 9.08 | 15.81 | 9.43 | 11.38 | 0.49 | 30.77 | 12.99 | 13.12 | 0.71 | 0.57 | 25.58 | 23.83 | 24.55 | 9.23 |
Mitotic Spindle | -2.76 | -17.73 | -7.32 | -5.29 | -1.92 | -1.37 | -19.10 | -3.59 | -3.50 | 4.69 | -2.72 | -25.83 | -8.18 | -7.85 | 0.86 |
None | 1.84 | 1.49 | 1.93 | 0.62 | 4.68 | 0.03 | 0.04 | 2.48 | 1.35 | 2.04 | 0.71 | 1.26 | 2.77 | 1.10 | 3.69 |
Nuclear Periphery | 1.14 | -4.80 | 0.11 | -1.59 | 5.93 | 1.90 | -5.76 | -0.47 | -0.85 | 3.97 | 2.32 | -8.55 | -1.15 | -2.92 | 7.28 |
Nuclear Periphery Foci | 1.09 | 0.21 | 0.94 | -2.31 | 5.00 | 1.64 | -3.30 | 0.26 | -1.06 | 3.33 | 1.66 | -1.57 | 1.11 | -2.37 | 6.13 |
Nucleolus | 0.44 | 2.12 | 2.04 | 1.33 | -0.17 | 2.16 | -1.59 | 2.70 | 1.15 | 2.70 | 2.58 | 0.59 | 2.24 | -0.26 | 1.64 |
Nucleus | -0.65 | 1.71 | -0.10 | 0.54 | -1.21 | 2.35 | -3.59 | 1.53 | -1.87 | 4.28 | 1.60 | -1.42 | -0.73 | -1.34 | -0.12 |
Peroxisomes | -5.57 | 3.34 | -6.61 | -0.38 | -8.23 | 4.10 | -5.41 | -4.19 | -5.06 | -2.66 | 1.19 | 1.45 | -7.12 | -7.68 | -7.80 |
Vacuole | 2.74 | -12.27 | -4.98 | -6.14 | -0.00 | -3.88 | -13.87 | -3.01 | -2.73 | 1.30 | -0.04 | -23.49 | -6.89 | -6.90 | 0.62 |
Vacuole Periphery | 5.57 | -8.76 | 2.85 | -3.49 | 14.57 | -1.88 | -5.42 | 0.27 | 1.06 | 2.98 | 1.89 | -15.58 | 0.63 | -0.68 | 13.87 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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