Standard name
Human Ortholog
Description Component of the spindle-assembly checkpoint complex; delays onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.84 0.98 0.96 0.89 0.91 0.8 0.83 – 0.73 0.66 0.58 0.6 0.88 0.85 0.82 0.45 0.41 0.41 0.82 0.86 0.82 0.83 0.75 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.12 0.12 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.19 0.09 – 0.25 0.29 0.42 0.42 0 0 0 0 0 0 0 0 0.07 0.05 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07
Vac/Vac Membrane 0.19 0 0 0.09 0.08 0 0.14 – 0.12 0.12 0.11 0.08 0.12 0.16 0.24 0.54 0.61 0.57 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 – 0 0 0 0 1 0 0 1 0 0 3 0 0 0 6 7
Bud 0 0 0 0 0 2 0 – 4 9 2 4 0 1 0 0 0 0 0 0 0 0 17 7
Bud Neck 0 0 0 0 0 1 2 – 3 1 0 1 0 0 0 0 0 0 1 1 1 0 3 9
Bud Site 0 0 0 0 0 0 0 – 0 0 0 1 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 1 1 1 2 1 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 269 159 163 192 277 163 393 – 280 316 209 246 240 384 410 101 57 35 288 162 162 171 383 311
Endoplasmic Reticulum 2 3 5 1 5 8 0 – 0 1 0 0 1 0 0 6 2 4 6 0 3 3 7 8
Endosome 10 0 1 5 4 0 4 – 4 7 5 7 9 20 16 14 17 10 16 3 4 5 17 17
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0
Mitochondria 2 1 0 4 4 39 43 – 97 139 152 172 2 3 2 3 2 2 4 4 13 9 16 9
Nucleus 1 0 1 1 2 6 5 – 13 10 13 11 2 0 2 7 2 0 3 2 1 1 3 4
Nuclear Periphery 0 0 0 0 0 1 0 – 1 1 0 1 0 1 0 0 0 0 3 1 1 0 5 5
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 0 1 3 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 1 0 0 6 – 5 1 2 3 0 1 2 0 0 0 7 2 3 3 27 27
Vac/Vac Membrane 62 0 2 20 25 4 69 – 45 58 38 31 33 71 122 122 85 49 8 5 1 2 8 8
Unique Cell Count 321 162 170 216 306 203 476 384 481 361 410 274 453 501 226 139 86 353 189 198 206 510 425
Labelled Cell Count 346 164 173 225 319 226 522 452 544 421 477 289 487 556 254 165 100 353 189 198 206 510 425


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 4.9 5.8 5.4 6.0 3.9 4.9 – 4.3 4.3 4.2 4.2 5.9 5.7 6.2 7.4 8.1 7.7 5.2 5.5 5.7
Std Deviation (1e-4) 0.6 0.6 1.0 1.3 1.5 1.0 1.1 – 1.0 0.9 1.0 0.7 1.2 1.5 1.4 1.2 1.3 1.2 1.6 1.4 1.8
Intensity Change (Log2) – – – -0.09 0.04 -0.56 -0.25 – -0.44 -0.42 -0.46 -0.47 0.02 -0.02 0.08 0.35 0.48 0.41 -0.17 -0.09 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 11.2 11.7 10.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.616 -0.0594 -0.2491 -0.8036 -0.9523 -0.3047 0.2879 0.7297 1.0558 0.2371 0.8135 0.8857 3.2993 3.2003 2.8372 3.0027 2.24 2.8988
Actin 0.0164 0.0002 0.0163 0.0015 0.0002 0.0138 0.0265 0.0073 0.0171 0.041 0.0558 0.003 0.0763 0.0002 0.0169 0.0004 0.0431 0.0101
Bud 0.0003 0.0002 0.0106 0.0287 0 0.0003 0.0003 0.0001 0.0058 0.0007 0.0084 0.0001 0.0015 0 0.0016 0.0001 0.0012 0
Bud Neck 0.0001 0.0003 0.0003 0.0003 0.0002 0.0016 0.0004 0.0001 0.0005 0.0005 0.0011 0.0006 0.009 0.0001 0.0021 0.0001 0.0003 0.0008
Bud Periphery 0.0006 0.0001 0.0024 0.0063 0 0.0003 0.0004 0 0.0013 0.0021 0.0079 0.0001 0.0019 0 0.0018 0.0001 0.0017 0.0001
Bud Site 0.0008 0.0022 0.0055 0.0032 0.0001 0.0005 0.0009 0.0004 0.0026 0.0014 0.0028 0.0001 0.0191 0.0003 0.0067 0.0001 0.0021 0.0001
Cell Periphery 0.0001 0.0001 0.0002 0.0004 0 0.0001 0.0001 0.0001 0.0002 0.0003 0.0003 0 0.0007 0.0001 0.0003 0 0.0001 0
Cytoplasm 0.3061 0.4744 0.3154 0.3779 0.5699 0.4804 0.4794 0.6606 0.6495 0.656 0.4929 0.7855 0.3045 0.6074 0.5416 0.4018 0.6429 0.6454
Cytoplasmic Foci 0.0104 0.0139 0.0177 0.0151 0.0053 0.0191 0.0298 0.0053 0.0061 0.0041 0.1242 0.007 0.0322 0.0046 0.0055 0.0023 0.0074 0.0072
Eisosomes 0.0003 0 0.0002 0.0002 0 0.0002 0.0003 0.0001 0.0002 0.0014 0.001 0 0.0006 0 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0053 0.0072 0.0071 0.0095 0.0037 0.0081 0.0135 0.0048 0.006 0.0082 0.0028 0.0124 0.0065 0.004 0.0061 0.0011 0.0023 0.0028
Endosome 0.0061 0.0041 0.0038 0.0104 0.0024 0.0075 0.0154 0.002 0.0058 0.0184 0.0735 0.0057 0.0235 0.0016 0.006 0.0005 0.0031 0.0016
Golgi 0.0015 0.0002 0.0012 0.0005 0.0001 0.0023 0.0035 0.0004 0.0054 0.0032 0.0243 0.0037 0.0096 0.0001 0.0011 0 0.0036 0.0006
Lipid Particles 0.0031 0.0007 0.0057 0.0046 0.0001 0.0016 0.014 0.0012 0.0023 0.0008 0.0103 0.0117 0.013 0.0002 0.0019 0.0001 0.0041 0.0019
Mitochondria 0.0029 0.0003 0.0017 0.0015 0.0002 0.0017 0.0028 0.0001 0.0042 0.0208 0.0813 0.0015 0.0036 0.0001 0.0006 0.0001 0.0017 0.0003
None 0.6214 0.4721 0.5748 0.4829 0.3784 0.4245 0.3746 0.3048 0.2767 0.1894 0.0664 0.1526 0.4353 0.3763 0.3929 0.5596 0.2723 0.3059
Nuclear Periphery 0.0115 0.0042 0.0048 0.0136 0.0032 0.0063 0.0098 0.0031 0.0038 0.0172 0.0024 0.0038 0.0102 0.0011 0.0024 0.0016 0.0014 0.0025
Nucleolus 0.0002 0.0003 0.0005 0.0024 0.0001 0.0005 0.002 0.0001 0.0002 0.0003 0.0004 0.0001 0.0009 0 0.0005 0.0001 0.0001 0.0001
Nucleus 0.0046 0.012 0.0064 0.0255 0.0335 0.0215 0.0088 0.0044 0.0069 0.0163 0.0035 0.0075 0.0055 0.0025 0.0057 0.0302 0.0027 0.0101
Peroxisomes 0.003 0.0001 0.0085 0.0005 0 0.0005 0.0064 0.0022 0.0021 0.0005 0.0278 0.0006 0.0305 0 0.0011 0.0001 0.0072 0.0069
Punctate Nuclear 0.0023 0.0058 0.0155 0.0118 0.0013 0.0077 0.0086 0.0023 0.0021 0.0013 0.0046 0.0026 0.0126 0.0006 0.0034 0.0013 0.0016 0.003
Vacuole 0.002 0.0015 0.0013 0.0025 0.0009 0.0012 0.0018 0.0008 0.001 0.0087 0.0053 0.001 0.002 0.0007 0.0013 0.0002 0.0008 0.0004
Vacuole Periphery 0.0011 0.0002 0.0003 0.0009 0.0001 0.0004 0.0007 0.0001 0.0003 0.0072 0.0028 0.0005 0.001 0.0001 0.0002 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.9361 5.007 7.4308 16.1025 9.6827 20.5258 10.547 13.0981 14.8632 15.7823
Translational Efficiency 2.0245 4.6612 3.015 1.5148 2.0291 1.1027 2.3367 1.5556 1.6001 1.5984

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2144 1433 1382 158 1943 2013 1887 1520 4087 3446 3269 1678

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 729.14 832.78 930.51 938.65 705.10 786.25 873.37 971.53 717.71 805.60 897.53 968.43
Standard Deviation 77.20 231.38 104.36 135.97 82.43 92.73 104.74 141.67 80.62 166.77 108.32 141.47
Intensity Change Log 2 — 0.191740 0.351826 0.364391 — 0.157160 0.308765 0.462431 — 0.174843 0.330817 0.413422

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000072 0.001491 0.000432 0.000501 0.000066 0.000157 0.000161 0.001021 0.000069 0.000712 0.000276 0.000972
Bud Neck 0.008389 0.012602 0.003317 0.009923 0.004987 0.015246 0.001680 0.009553 0.006772 0.014146 0.002372 0.009588
Bud Site 0.002032 0.051519 0.002898 0.008835 0.001645 0.010756 0.003189 0.024117 0.001848 0.027707 0.003066 0.022678
Cell Periphery 0.000082 0.012554 0.000091 0.000168 0.000081 0.000248 0.000078 0.000154 0.000081 0.005365 0.000084 0.000156
Cytoplasm 0.473736* 0.468559* 0.342929* 0.343114* 0.580007* 0.475056* 0.536082* 0.393167* 0.524259* 0.472354* 0.454425* 0.388454*
Cytoplasmic Foci 0.197688* 0.182503* 0.005297 0.010693 0.137165 0.128636 0.008147 0.022518 0.168915 0.151036 0.006942 0.021405
Eisosomes 0.000102 0.000202 0.000023 0.000054 0.000055 0.000092 0.000019 0.000033 0.000080 0.000138 0.000021 0.000035
Endoplasmic Reticulum 0.004047 0.007318 0.004810 0.011306 0.002251 0.002227 0.003101 0.007149 0.003193 0.004344 0.003824 0.007541
Endosome 0.007457 0.013962 0.001950 0.008630 0.004989 0.013025 0.001392 0.010801 0.006284 0.013415 0.001628 0.010597
Golgi 0.001640 0.001816 0.000081 0.000747 0.000876 0.001463 0.000121 0.004081 0.001276 0.001610 0.000104 0.003767
Lipid Particles 0.022202 0.013320 0.001150 0.004113 0.010086 0.009916 0.000976 0.002637 0.016442 0.011331 0.001049 0.002776
Mitochondria 0.004397 0.002370 0.000872 0.016833 0.001311 0.003987 0.000422 0.004920 0.002930 0.003315 0.000612 0.006042
Mitotic Spindle 0.002354 0.002136 0.004514 0.064979 0.002409 0.003758 0.007118 0.124398* 0.002380 0.003083 0.006017 0.118804*
None 0.003515 0.006262 0.005255 0.008215 0.006893 0.009875 0.006771 0.009285 0.005121 0.008372 0.006130 0.009185
Nuclear Periphery 0.003004 0.002530 0.003589 0.010471 0.001491 0.002082 0.003142 0.015050 0.002284 0.002268 0.003331 0.014618
Nuclear Periphery Foci 0.002243 0.005772 0.003219 0.072482 0.002584 0.001022 0.003847 0.027074 0.002405 0.002997 0.003581 0.031349
Nucleolus 0.001467 0.002187 0.000616 0.003189 0.001770 0.003139 0.000309 0.002275 0.001611 0.002743 0.000439 0.002361
Nucleus 0.230426* 0.162725* 0.585073* 0.291345* 0.219065* 0.256699* 0.381570* 0.265931* 0.225025* 0.217620* 0.467603* 0.268324*
Peroxisomes 0.003731 0.003874 0.000276 0.000529 0.001394 0.004200 0.000160 0.002657 0.002620 0.004065 0.000209 0.002457
Vacuole 0.030716 0.044956 0.033456 0.132423 0.020443 0.056661 0.041641 0.070457 0.025832 0.051793 0.038181 0.076291
Vacuole Periphery 0.000701 0.001344 0.000150 0.001449 0.000432 0.001755 0.000074 0.002720 0.000573 0.001584 0.000106 0.002600

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.11 -4.02 -6.94 1.12 -2.94 -3.25 -9.53 -8.57 -7.18 -6.37 -3.37 -6.19 -9.59 -0.37 -5.12
Bud Neck -2.74 4.53 -2.80 -1.77 -3.82 -7.66 2.89 -7.53 1.54 -12.44 -7.34 5.80 -7.16 0.27 -11.94
Bud Site -10.53 -1.85 -3.51 6.23 -3.14 -6.42 -1.95 -7.21 -1.16 -6.09 -12.03 -2.58 -7.75 5.51 -6.72
Cell Periphery -5.17 -3.60 -5.46 5.13 -4.20 -5.23 -0.00 -5.79 3.62 -5.68 -5.24 -2.33 -7.25 5.19 -5.75
Cytoplasm 0.56 21.10 13.31 12.61 4.34 12.87 15.40 33.45 20.22 17.92 8.45 22.46 32.24 23.75 13.02
Cytoplasmic Foci 2.41 44.72 43.26 33.35 -2.83 1.65 34.83 32.10 30.69 -4.02 4.36 55.80 50.69 43.08 -5.36
Eisosomes -2.33 16.79 0.85 2.48 -7.37 -7.71 11.78 3.19 9.78 -9.86 -3.20 20.52 10.26 4.81 -11.24
Endoplasmic Reticulum -4.36 -6.58 -6.67 -4.46 -4.71 0.18 -8.07 -16.01 -16.12 -10.15 -3.07 -8.91 -14.72 -10.83 -9.00
Endosome -5.56 10.35 -0.73 3.88 -5.81 -7.69 9.19 -2.60 5.51 -8.73 -9.07 14.03 -0.99 7.26 -9.19
Golgi -0.74 9.56 5.64 6.11 -3.00 -2.02 5.50 -0.89 0.57 -3.10 -1.70 10.83 0.35 1.25 -3.22
Lipid Particles 6.08 18.07 14.60 9.07 -5.43 0.19 11.68 9.17 8.21 -10.90 5.50 21.34 18.46 13.07 -12.00
Mitochondria 3.84 7.14 -0.04 -0.63 -1.05 -5.29 5.49 -3.76 2.35 -6.49 -0.97 8.43 0.26 0.99 -4.82
Mitotic Spindle 0.08 -2.53 -4.83 -4.84 -4.56 -1.13 -3.31 -17.57 -17.37 -16.46 -0.97 -4.19 -18.27 -18.16 -17.31
None -2.69 -2.57 -3.16 -1.75 -2.53 -2.24 0.66 -2.11 0.79 -3.70 -3.78 -1.44 -5.49 -0.68 -5.23
Nuclear Periphery 1.16 -6.23 -4.60 -4.79 -3.67 -1.67 -11.67 -15.49 -14.66 -12.76 0.09 -11.64 -15.44 -15.28 -13.20
Nuclear Periphery Foci -4.65 -3.55 -4.52 -4.15 -4.27 3.25 -3.74 -11.06 -12.09 -9.66 -1.28 -5.31 -12.15 -11.90 -10.81
Nucleolus -1.89 3.50 -3.04 -2.68 -3.31 -2.28 3.04 -4.73 -2.33 -9.78 -3.20 4.39 -7.06 -4.21 -9.98
Nucleus 10.44 -44.45 -4.03 -6.75 10.76 -5.62 -27.77 -10.89 -5.65 12.95 1.50 -47.90 -11.57 -11.93 23.38
Peroxisomes -0.27 10.54 9.12 8.35 -3.48 -4.78 8.15 1.66 5.36 -4.82 -3.53 12.96 6.67 7.69 -4.83
Vacuole -6.26 -9.21 -9.35 -8.61 -7.70 -14.19 -18.17 -24.05 -16.48 -11.99 -14.42 -19.34 -24.90 -19.53 -15.27
Vacuole Periphery -4.48 7.58 0.64 2.87 -1.46 -5.60 8.27 -1.78 2.47 -3.17 -6.68 10.91 -1.21 2.61 -3.18
Standard name
Human Ortholog
Description Component of the spindle-assembly checkpoint complex; delays onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
Localization
Cell Percentages cytoplasm (24%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mad2

Mad2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mad2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available