Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress; SVL3 has a paralog, PAM1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 54 | 60 | 12 | – | 14 | 14 | 29 | 17 | 15 | 22 | 12 | 14 | 37 | 35 | 20 | 25 | 23 | 21 | 36 | 38 | 13 | 36 | 65 | 84 |
Bud | 29 | 43 | 2 | – | 8 | 6 | 21 | 15 | 25 | 21 | 15 | 13 | 12 | 6 | 7 | 21 | 10 | 18 | 83 | 103 | 25 | 54 | 53 | 47 |
Bud Neck | 1 | 1 | 1 | – | 0 | 0 | 0 | 4 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 28 | 40 | 15 | 39 | 36 | 36 |
Bud Site | 12 | 11 | 5 | – | 3 | 9 | 12 | 3 | 7 | 2 | 2 | 6 | 3 | 1 | 1 | 2 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 21 | 19 | 2 | – | 5 | 3 | 9 | 19 | 21 | 26 | 17 | 11 | 24 | 97 | 87 | 10 | 11 | 4 | 10 | 10 | 2 | 9 | 31 | 38 |
Cytoplasm | 33 | 56 | 13 | – | 25 | 17 | 52 | 81 | 66 | 75 | 50 | 59 | 59 | 92 | 114 | 37 | 25 | 29 | 9 | 23 | 17 | 18 | 23 | 55 |
Endoplasmic Reticulum | 30 | 31 | 3 | – | 6 | 3 | 8 | 17 | 9 | 7 | 5 | 6 | 46 | 106 | 65 | 1 | 4 | 1 | 20 | 15 | 2 | 12 | 12 | 25 |
Endosome | 1 | 6 | 0 | – | 0 | 1 | 1 | 0 | 0 | 1 | 2 | 1 | 1 | 1 | 2 | 4 | 0 | 2 | 1 | 5 | 0 | 2 | 1 | 1 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 14 | 3 | 0 | – | 2 | 7 | 25 | 14 | 46 | 51 | 49 | 34 | 0 | 1 | 1 | 6 | 0 | 1 | 5 | 4 | 4 | 4 | 2 | 5 |
Nucleus | 13 | 6 | 2 | – | 4 | 2 | 5 | 10 | 11 | 7 | 6 | 10 | 6 | 2 | 9 | 1 | 2 | 1 | 5 | 3 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 4 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 |
Nucleolus | 3 | 5 | 2 | – | 2 | 2 | 1 | 0 | 4 | 11 | 4 | 5 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | – | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 5 | 2 | 2 | – | 1 | 0 | 2 | 4 | 0 | 5 | 3 | 1 | 0 | 1 | 1 | 1 | 5 | 0 | 1 | 6 | 2 | 0 | 0 | 0 |
Vac/Vac Membrane | 18 | 40 | 9 | – | 0 | 1 | 1 | 16 | 13 | 15 | 10 | 9 | 1 | 11 | 25 | 20 | 12 | 14 | 13 | 17 | 4 | 3 | 5 | 5 |
Unique Cell Count | 169 | 207 | 42 | 58 | 46 | 117 | 154 | 153 | 177 | 121 | 128 | 152 | 263 | 250 | 99 | 74 | 70 | 221 | 274 | 94 | 188 | 235 | 310 | |
Labelled Cell Count | 235 | 283 | 53 | 71 | 65 | 166 | 202 | 220 | 249 | 177 | 170 | 190 | 354 | 336 | 132 | 97 | 91 | 221 | 274 | 94 | 188 | 235 | 310 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.5 | 9.5 | 8.4 | – | 8.4 | 6.3 | 7.0 | 7.6 | 6.3 | 6.3 | 6.1 | 6.2 | 9.6 | 9.5 | 9.2 | 12.3 | 12.8 | 11.6 | 9.0 | 8.4 | 8.4 |
Std Deviation (1e-4) | 2.9 | 2.7 | 1.9 | – | 1.9 | 1.2 | 1.6 | 1.9 | 1.9 | 1.7 | 1.5 | 1.5 | 1.9 | 1.5 | 1.6 | 3.6 | 3.6 | 3.1 | 1.7 | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | – | 0.01 | -0.42 | -0.26 | -0.14 | -0.4 | -0.4 | -0.47 | -0.44 | 0.21 | 0.19 | 0.14 | 0.56 | 0.62 | 0.47 | 0.1 | -0.0 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | – | -0.5 | 0.2 | -0.5 | -2.8 | -3.1 | -2.6 | -2.9 | -2.8 | -0.6 | -2.5 | 0 | -0.4 | 0.3 | 0.2 |
Bud | – | 0 | 0 | 2.1 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 | 2.8 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | – | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 0 | 1.9 | 4.1 | 3.9 | 0 | 0 | 0 |
Cytoplasm | – | 1.2 | 0.6 | 1.5 | 2.5 | 1.4 | 1.4 | 1.2 | 1.7 | 0.9 | 0.5 | 1.8 | 0.7 | 0.3 | 1.1 |
Endoplasmic Reticulum | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 4.2 | 2.7 | 0 | 0 | 0 |
Endosome | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | – | 0 | 0 | 3.3 | 0 | 4.1 | 4.0 | 4.9 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | – | 0 | 0 | 0 | -1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.2 | -0.7 | -0.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.4512 | 7.4362 | 8.1643 | 8.3679 | 7.0856 | 9.1246 | 7.4433 | 6.6224 | 6.9732 | 8.9765 | 7.2048 | 7.9815 | 6.913 | 6.4205 | 6.1773 | 7.2118 | 6.1406 | 7.2727 |
Actin | 0.0943 | 0.014 | 0.0599 | 0.0127 | 0.1032 | 0.0449 | 0.1101 | 0.0325 | 0.014 | 0.0066 | 0.016 | 0.0667 | 0.1157 | 0.0428 | 0.0161 | 0.0294 | 0.0891 | 0.0431 |
Bud | 0.0469 | 0.0454 | 0.3164 | 0.1374 | 0.1077 | 0.0132 | 0.0369 | 0.0785 | 0.2706 | 0.113 | 0.1212 | 0.0188 | 0.0221 | 0.0328 | 0.3123 | 0.1764 | 0.1127 | 0.0244 |
Bud Neck | 0.0667 | 0.0339 | 0.0064 | 0.0027 | 0.0692 | 0.6344 | 0.066 | 0.0181 | 0.0083 | 0.0047 | 0.0137 | 0.5588 | 0.0976 | 0.0235 | 0.0136 | 0.0044 | 0.001 | 0.568 |
Bud Periphery | 0.1375 | 0.1051 | 0.4377 | 0.7202 | 0.6293 | 0.0741 | 0.1387 | 0.1152 | 0.5016 | 0.7383 | 0.6648 | 0.1132 | 0.0623 | 0.0674 | 0.4587 | 0.5669 | 0.6547 | 0.1392 |
Bud Site | 0.2049 | 0.5414 | 0.0958 | 0.0177 | 0.0112 | 0.0132 | 0.1618 | 0.5409 | 0.123 | 0.0076 | 0.0168 | 0.0153 | 0.2103 | 0.5346 | 0.086 | 0.0378 | 0.0134 | 0.0221 |
Cell Periphery | 0.1648 | 0.0467 | 0.0124 | 0.0237 | 0.0193 | 0.0532 | 0.1616 | 0.029 | 0.0105 | 0.0602 | 0.0533 | 0.0607 | 0.1534 | 0.0316 | 0.0164 | 0.0192 | 0.0168 | 0.0479 |
Cytoplasm | 0.1517 | 0.1486 | 0.0127 | 0.0049 | 0.0013 | 0.1245 | 0.1757 | 0.0868 | 0.0107 | 0.0103 | 0.007 | 0.0991 | 0.1463 | 0.1845 | 0.032 | 0.0239 | 0.0015 | 0.1046 |
Cytoplasmic Foci | 0.0128 | 0.0009 | 0.0056 | 0.0171 | 0.0066 | 0.0021 | 0.0265 | 0.002 | 0.0025 | 0.0002 | 0.0023 | 0.0031 | 0.0232 | 0.0042 | 0.0015 | 0.0352 | 0.0008 | 0.0071 |
Eisosomes | 0.0022 | 0.0005 | 0.0021 | 0.0003 | 0.0005 | 0.0002 | 0.002 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0004 | 0.0024 | 0.0006 | 0.0001 | 0.0001 | 0.0005 | 0.0003 |
Endoplasmic Reticulum | 0.0213 | 0.0044 | 0.0054 | 0.0079 | 0.0014 | 0.0083 | 0.0216 | 0.0056 | 0.0028 | 0.0034 | 0.0024 | 0.0098 | 0.0281 | 0.0059 | 0.0055 | 0.0041 | 0.0018 | 0.0099 |
Endosome | 0.0165 | 0.0005 | 0.0067 | 0.0014 | 0.0056 | 0.0025 | 0.0239 | 0.0008 | 0.0082 | 0.0007 | 0.0148 | 0.0068 | 0.0341 | 0.0012 | 0.0022 | 0.0053 | 0.0057 | 0.0012 |
Golgi | 0.006 | 0.0003 | 0.0056 | 0.0013 | 0.0104 | 0.0012 | 0.0132 | 0.0007 | 0.0061 | 0.0001 | 0.0074 | 0.0051 | 0.0227 | 0.0006 | 0.0025 | 0.0013 | 0.002 | 0.001 |
Lipid Particles | 0.0089 | 0.0003 | 0.006 | 0.0098 | 0.0097 | 0.0015 | 0.0147 | 0.0003 | 0.0014 | 0.0001 | 0.003 | 0.0036 | 0.0115 | 0.0014 | 0.009 | 0.0009 | 0.0007 | 0.0016 |
Mitochondria | 0.0028 | 0.0004 | 0.0062 | 0.0064 | 0.0012 | 0.0006 | 0.0047 | 0.0075 | 0.0104 | 0.0005 | 0.0128 | 0.0054 | 0.0047 | 0.0009 | 0.0124 | 0.0014 | 0.078 | 0.0004 |
None | 0.0092 | 0.0011 | 0.0044 | 0.0011 | 0.0006 | 0.0009 | 0.0024 | 0.0013 | 0.0006 | 0.0001 | 0.0003 | 0.001 | 0.0166 | 0.0127 | 0.0004 | 0.0002 | 0.0018 | 0.0098 |
Nuclear Periphery | 0.013 | 0.0027 | 0.0023 | 0.0024 | 0.0014 | 0.0053 | 0.0058 | 0.0042 | 0.0071 | 0.0023 | 0.0014 | 0.0058 | 0.0088 | 0.0036 | 0.0017 | 0.0042 | 0.0049 | 0.0032 |
Nucleolus | 0.0044 | 0.0024 | 0.0007 | 0.0003 | 0.0002 | 0.0006 | 0.0038 | 0.0018 | 0.0009 | 0.0013 | 0.002 | 0.0061 | 0.0049 | 0.0009 | 0.0009 | 0.0005 | 0.0012 | 0.0006 |
Nucleus | 0.0089 | 0.0395 | 0.0034 | 0.004 | 0.0005 | 0.0112 | 0.0034 | 0.0595 | 0.0115 | 0.0152 | 0.0529 | 0.0066 | 0.0064 | 0.0404 | 0.0228 | 0.0432 | 0.0058 | 0.0055 |
Peroxisomes | 0.0032 | 0.0001 | 0.0041 | 0.0187 | 0.0141 | 0.0004 | 0.0097 | 0.0003 | 0.0008 | 0 | 0.0009 | 0.0008 | 0.0036 | 0.0007 | 0.001 | 0.0134 | 0.0014 | 0.0007 |
Punctate Nuclear | 0.0148 | 0.0018 | 0.0019 | 0.0016 | 0.0037 | 0.0016 | 0.0075 | 0.0091 | 0.0017 | 0.0001 | 0.0003 | 0.002 | 0.0121 | 0.0047 | 0.0005 | 0.003 | 0.0006 | 0.0045 |
Vacuole | 0.0085 | 0.0094 | 0.0037 | 0.0064 | 0.0024 | 0.0055 | 0.0089 | 0.0053 | 0.0053 | 0.0328 | 0.0054 | 0.0099 | 0.0117 | 0.0045 | 0.0043 | 0.0247 | 0.0031 | 0.0046 |
Vacuole Periphery | 0.0008 | 0.0005 | 0.0007 | 0.0023 | 0.0004 | 0.0006 | 0.001 | 0.0003 | 0.0016 | 0.0025 | 0.0009 | 0.0007 | 0.0013 | 0.0004 | 0.0004 | 0.0046 | 0.0023 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 63.2298 | 58.9119 | 23.6298 | 30.1234 | 58.3099 | 49.3987 | 55.038 | 41.2894 | 23.1834 | 62.5686 |
Translational Efficiency | 1.1634 | 1.2137 | 0.9865 | 0.574 | 0.8545 | 1.1558 | 0.8444 | 0.7048 | 0.9221 | 0.779 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
833 | 1528 | 691 | 1127 | 1204 | 1784 | 1621 | 2724 | 2037 | 3312 | 2312 | 3851 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 877.66 | 966.49 | 1370.16 | 1251.16 | 872.83 | 1020.19 | 1243.77 | 1347.73 | 874.81 | 995.42 | 1281.54 | 1319.47 |
Standard Deviation | 125.17 | 183.91 | 203.59 | 203.71 | 171.61 | 162.12 | 217.36 | 232.78 | 154.34 | 174.58 | 221.05 | 228.92 |
Intensity Change Log 2 | — | 0.139093 | 0.642610 | 0.511532 | — | 0.225065 | 0.510947 | 0.626759 | — | 0.182601 | 0.578462 | 0.570137 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.016063 | 0.015315 | 0.024982 | 0.021035 | 0.016165 | 0.012953 | 0.008237 | 0.007495 | 0.016123 | 0.014042 | 0.013242 | 0.011458 |
Bud Neck | 0.169225 | 0.122147 | 0.195906* | 0.134382 | 0.128163 | 0.174688 | 0.139031 | 0.160886 | 0.144955 | 0.150448 | 0.156030 | 0.153130 |
Bud Site | 0.562819* | 0.595013* | 0.626889* | 0.584636* | 0.524946* | 0.613774* | 0.547786* | 0.477809* | 0.540434* | 0.605118* | 0.571428* | 0.509072* |
Cell Periphery | 0.048160 | 0.026974 | 0.042838 | 0.030913 | 0.072402 | 0.084302 | 0.029159 | 0.046081 | 0.062489 | 0.057854 | 0.033247 | 0.041642 |
Cytoplasm | 0.112433* | 0.107151* | 0.012247 | 0.073363 | 0.109477* | 0.054389 | 0.098571 | 0.140927* | 0.110686* | 0.078731 | 0.072771 | 0.121154* |
Cytoplasmic Foci | 0.013734 | 0.016184 | 0.001374 | 0.003667 | 0.026198 | 0.006370 | 0.006178 | 0.000986 | 0.021101 | 0.010898 | 0.004742 | 0.001771 |
Eisosomes | 0.001925 | 0.000965 | 0.003274 | 0.001854 | 0.002716 | 0.001702 | 0.001148 | 0.001177 | 0.002392 | 0.001362 | 0.001784 | 0.001375 |
Endoplasmic Reticulum | 0.009362 | 0.016260 | 0.019019 | 0.014577 | 0.019665 | 0.005971 | 0.012455 | 0.005132 | 0.015452 | 0.010718 | 0.014417 | 0.007896 |
Endosome | 0.002187 | 0.005067 | 0.000966 | 0.001200 | 0.005668 | 0.002067 | 0.001468 | 0.000600 | 0.004244 | 0.003451 | 0.001318 | 0.000776 |
Golgi | 0.001574 | 0.002265 | 0.000254 | 0.000869 | 0.002652 | 0.001218 | 0.000325 | 0.000123 | 0.002211 | 0.001701 | 0.000304 | 0.000341 |
Lipid Particles | 0.002268 | 0.001986 | 0.000820 | 0.000860 | 0.003854 | 0.000608 | 0.001517 | 0.000346 | 0.003205 | 0.001244 | 0.001308 | 0.000497 |
Mitochondria | 0.003889 | 0.004844 | 0.001880 | 0.002523 | 0.003908 | 0.002711 | 0.003391 | 0.005485 | 0.003900 | 0.003695 | 0.002940 | 0.004618 |
Mitotic Spindle | 0.000751 | 0.001320 | 0.000907 | 0.003248 | 0.001086 | 0.001245 | 0.002624 | 0.006909 | 0.000949 | 0.001280 | 0.002111 | 0.005837 |
None | 0.000496 | 0.001132 | 0.000126 | 0.000527 | 0.003595 | 0.000534 | 0.002563 | 0.001759 | 0.002328 | 0.000810 | 0.001834 | 0.001398 |
Nuclear Periphery | 0.000105 | 0.000083 | 0.000149 | 0.000195 | 0.000122 | 0.000043 | 0.000275 | 0.000225 | 0.000115 | 0.000062 | 0.000237 | 0.000216 |
Nuclear Periphery Foci | 0.000693 | 0.000932 | 0.000339 | 0.000877 | 0.001760 | 0.000234 | 0.000585 | 0.000052 | 0.001324 | 0.000556 | 0.000512 | 0.000293 |
Nucleolus | 0.000365 | 0.000467 | 0.000113 | 0.000077 | 0.000541 | 0.000283 | 0.000475 | 0.000225 | 0.000469 | 0.000368 | 0.000367 | 0.000182 |
Nucleus | 0.026718 | 0.044582 | 0.039429 | 0.060871 | 0.046711 | 0.019181 | 0.090252 | 0.094054 | 0.038535 | 0.030900 | 0.075062 | 0.084343 |
Peroxisomes | 0.002308 | 0.002622 | 0.000257 | 0.000347 | 0.002679 | 0.001249 | 0.000564 | 0.000381 | 0.002528 | 0.001882 | 0.000472 | 0.000371 |
Vacuole | 0.024532 | 0.033383 | 0.027913 | 0.063707 | 0.026824 | 0.016128 | 0.052942 | 0.049015 | 0.025887 | 0.024089 | 0.045462 | 0.053315 |
Vacuole Periphery | 0.000393 | 0.001310 | 0.000317 | 0.000270 | 0.000868 | 0.000350 | 0.000452 | 0.000331 | 0.000674 | 0.000793 | 0.000412 | 0.000313 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.30 | -2.66 | -0.37 | -0.82 | 2.63 | 1.74 | 5.15 | 6.16 | 4.88 | 2.14 | 1.37 | 2.19 | 4.58 | 3.90 | 3.40 |
Bud Neck | 4.75 | -1.28 | 6.08 | 1.86 | 8.52 | -5.48 | 0.24 | -1.44 | 5.17 | -2.06 | -0.82 | 0.24 | 2.55 | 4.09 | 2.73 |
Bud Site | -1.83 | -1.06 | 8.17 | 11.42 | 9.53 | -7.26 | 2.65 | 11.28 | 22.60* | 9.05 | -6.65 | 2.31 | 14.57 | 25.20* | 12.62 |
Cell Periphery | 4.38 | 1.69 | 5.47 | 1.81 | 3.98 | -2.00 | 9.27 | 7.22 | 10.82 | -3.54 | 1.13 | 8.82 | 8.49 | 9.07 | -0.75 |
Cytoplasm | 0.46 | 13.79 | 1.68 | 1.46 | -13.98 | 8.51 | 0.66 | -7.66 | -19.41 | -9.05 | 6.04 | 5.89 | -6.06 | -14.01 | -13.23 |
Cytoplasmic Foci | -1.24 | 6.79 | 4.95 | 7.97 | -3.64 | 8.19 | 8.40 | 10.83 | 6.44 | 7.10 | 5.99 | 10.11 | 12.16 | 11.24 | 4.78 |
Eisosomes | 3.53 | -1.58 | 1.91 | -2.07 | 2.55 | 2.58 | 4.86 | 5.29 | 3.27 | 0.96 | 4.07 | 2.66 | 5.80 | 1.90 | 2.73 |
Endoplasmic Reticulum | -3.19 | -4.18 | -2.94 | 0.52 | 2.17 | 6.79 | 2.30 | 7.16 | 0.53 | 8.89 | 3.02 | -0.70 | 4.94 | 2.63 | 7.77 |
Endosome | -4.41 | 2.86 | 2.04 | 6.05 | -0.78 | 4.61 | 5.78 | 7.23 | 3.71 | 4.86 | 1.47 | 6.24 | 7.69 | 7.50 | 3.98 |
Golgi | -1.23 | 3.79 | 1.99 | 2.95 | -0.93 | 2.65 | 5.64 | 6.37 | 2.96 | 1.43 | 1.29 | 6.67 | 6.66 | 4.74 | 0.24 |
Lipid Particles | 0.29 | 1.09 | 0.44 | 0.29 | -2.14 | 5.03 | 3.09 | 5.09 | -0.04 | 3.80 | 3.82 | 2.99 | 4.51 | 1.50 | 2.91 |
Mitochondria | -0.64 | 1.34 | 1.31 | 2.63 | 0.19 | 1.25 | 0.61 | -1.29 | -3.71 | -2.50 | 0.25 | 1.14 | -0.48 | -1.01 | -2.33 |
Mitotic Spindle | -1.55 | -1.28 | -3.97 | -3.46 | -3.58 | -0.34 | -3.21 | -5.63 | -5.41 | -2.47 | -1.13 | -3.45 | -6.89 | -6.40 | -3.67 |
None | -3.07 | 2.89 | -2.27 | 1.53 | -5.90 | 2.59 | 0.92 | 1.42 | -4.08 | 0.77 | 2.16 | 0.72 | 1.02 | -3.60 | 0.31 |
Nuclear Periphery | 0.33 | -3.59 | -7.65 | -11.33 | -4.90 | 4.57 | -9.96 | -9.93 | -12.00 | -2.51 | 2.21 | -9.28 | -11.86 | -15.33 | -4.36 |
Nuclear Periphery Foci | -1.24 | 1.20 | -2.18 | -1.30 | -3.64 | 5.02 | 3.30 | 5.48 | 2.90 | 7.44 | 3.76 | 3.33 | 4.32 | 1.23 | 2.31 |
Nucleolus | -0.49 | 0.98 | 1.06 | 2.67 | 0.63 | 0.88 | 0.53 | 2.43 | 0.27 | 0.82 | 0.45 | 0.69 | 2.20 | 1.18 | 0.85 |
Nucleus | -4.71 | -6.82 | -15.50 | -11.94 | -5.85 | 6.56 | -11.96 | -19.07 | -28.90 | -4.90 | 2.15 | -14.52 | -25.25 | -29.12 | -7.12 |
Peroxisomes | -0.50 | 5.12 | 4.92 | 4.37 | -1.60 | 3.59 | 6.26 | 6.75 | 3.44 | 1.18 | 1.77 | 7.89 | 8.25 | 5.42 | 0.91 |
Vacuole | -3.18 | -5.35 | -18.43 | -17.08 | -14.15 | 4.61 | -13.17 | -16.26 | -22.22 | 0.45 | 0.63 | -14.32 | -24.32 | -25.76 | -7.71 |
Vacuole Periphery | -3.62 | 0.37 | 1.44 | 4.57 | 1.82 | 4.31 | 3.41 | 4.71 | 0.79 | 1.27 | -0.89 | 2.93 | 4.56 | 4.38 | 1.72 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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